Application of Multi-Omics Analysis to Fish Nutrition, Health and Welfare

A special issue of Animals (ISSN 2076-2615). This special issue belongs to the section "Aquatic Animals".

Deadline for manuscript submissions: 31 August 2026 | Viewed by 566

Special Issue Editor


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Guest Editor
Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China
Interests: aquaculture animal diseases; etiology; pathogenic mechanism; antibiotic; vaccine; prevention; treatment
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Special Issue Information

Dear Colleagues,

Aquaculture serves as a crucial pillar for global food security, yet the industry faces persistent challenges regarding feed optimization, disease outbreaks, and environmental stress. To address these complexities, researchers are increasingly transitioning from single-parameter studies to systems biology approaches. This Special Issue, titled “Application of Multi-Omics Analysis to Fish Nutrition, Health and Welfare”, aims to highlight the latest advances in the application of high-throughput technologies—including genomics, transcriptomics, proteomics, metabolomics, and microbiomics—to aquatic biology.

We invite submissions that utilize these integrated approaches to elucidate the molecular mechanisms underlying fish growth, reproductive physiology, nutrient metabolism, and adaptive responses to environmental stressors. The scope of this issue extends to assessing fish welfare through molecular biomarkers and understanding the complex interactions between the host, its diet, and the microbiome. By bridging the gap between large-scale molecular data and phenotypic traits, this collection aims to provide a robust theoretical basis for precision nutrition, stress management, and improved welfare standards in sustainable aquaculture. We welcome both original research articles and comprehensive reviews.

Dr. Jin Xu
Guest Editor

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Keywords

  • multi-omics
  • aquaculture
  • fish nutrition
  • environmental stress
  • reproduction
  • animal welfare
  • systems biology
  • gut microbiota

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Published Papers (1 paper)

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Research

20 pages, 3857 KB  
Article
Dietary Supplementation with Methionine and Lysine Enhances Antioxidant Function and Muscle Quality of Hefang Crucian Carp (Carassius auratus)
by Xiao Chen, Yiren Wang, Xubing Wang, Minggui Jiang, Hui Li, Xingyu Huang, Hanyuan Wang, Qianhong Gu, Yonghua Zhou and Yamei Xiao
Animals 2026, 16(11), 1636; https://doi.org/10.3390/ani16111636 - 27 May 2026
Viewed by 225
Abstract
Methionine (Met) and lysine (Lys), as primary limiting amino acids, play important roles in regulating muscle quality in aquatic animals. This study investigated the effects of dietary Met and Lys supplementation on the growth performance, antioxidant function, and muscle quality of Hefang crucian [...] Read more.
Methionine (Met) and lysine (Lys), as primary limiting amino acids, play important roles in regulating muscle quality in aquatic animals. This study investigated the effects of dietary Met and Lys supplementation on the growth performance, antioxidant function, and muscle quality of Hefang crucian carp (HCC) using physiological and transcriptomic analyses. Fish were fed three diets for 8 weeks: a basal diet (LA) and two diets supplemented with DL-methionine at 1.7% (MA) and 3.4% (HA), respectively, while L-lysine supplementation was fixed at 3.4%. The results indicated that dietary Met and Lys supplementation had no significant effect on the growth performance of HCC (p > 0.05), but significantly reduced serum triglyceride (TG) and cholesterol levels (T-CHO) (p < 0.05). Additionally, serum total protein (TP) content was significantly increased in the MA group (p < 0.05). Analysis of serum antioxidant enzyme activities indicated that appropriate Met and Lys supplementation improved antioxidant capacity and upregulated the expression of antioxidant-related genes (Nrf2, GPX1a, GSTO1, GSTP1) in the muscle. Moreover, the MA group exhibited superior muscle hardness and gumminess, while the HA group had higher springiness and chewiness (p < 0.05). Muscle fiber density was significantly increased, whereas diameter and area showed opposite trends in fish fed 1.7% Met and 3.4% Lys (p < 0.05). Furthermore, appropriate Met and Lys supplementation significantly affected muscle fiber development genes (MyoD, MyoG, and MRF4), with MyoG highest in the HA group and MyoD and MRF4 highest in the MA group (p < 0.05). Comparative transcriptomic analysis of muscle tissue showed DEGs were mainly enriched in pathways correlated with muscle quality, involving cardiac muscle contraction, actin cytoskeleton regulation, PPAR signaling pathway and ECM–receptor interaction. Taken together, our findings enhance the understanding of the molecular mechanisms underlying the effects of dietary Met and Lys on muscle quality in HCC, providing valuable insights for the development of nutritional strategies in the aquaculture industry. Full article
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