Search Results (109)

Climate change can severely impact plant-pollinator interactions and have serious effects on ecosystem services such as pollination. This study was carried out in 2023 and 2024, and it examined the effects of drought on flowering and nectar production in one cultivar of white mustard (Brassica alba cv. Palma), an important entomophilous crop of the temperate zone with several attributes that make it promising for sustainable agricultural practices. Drought-stressed plants delayed the flowering time, shortened the flowering duration, and developed significantly fewer flowers. Nectar production in white mustard depends on soil moisture levels and short-term changes in meteorological conditions (e.g., air humidity, air temperature). At reduced soil moisture, the total sugar yield per plant decreased by 60%, compared to control plants, resulting in lower availability of caloric food resources, which should be considered when developing strategies supporting pollinators. Changes in floral traits resulted in differences in the frequency of insect visits, which may exert a negative impact on white mustard pollination under drought stress and may have indirect consequences for seed yield resulting from increased drought intensity associated with climate change. The results provide important data for the management of the white mustard crop and indicate the need for broader evaluation of cultivars to promote drought-resistant B. alba cultivars. Full article

A bioassay study was conducted to determine the differences in the sensitivity of selected crops to simulated clomazone residues (nine concentrations were used ranging from 5.625 to 1440 μg a.i./kg soil). White mustard was the most susceptible as measured by shoot fresh weight (SFW) and shoot dry weight (SDW) inhibition, with EC₅₀ values of 94.6 and 128.2 μg a.i./kg soil, respectively. Regarding the EC₅₀ values for the inhibition of pigment content (carotenoids, chlorophyll a and chlorophyll b), sugar beet and white mustard showed a high sensitivity, as the EC₅₀ values for all three pigments were in the range of 45.8–47.4 and 57.5–63.3 μg a.i./kg soil, respectively. However, as the SFW and SDW of sugar beet were only reduced at the three highest clomazone concentrations applied, white mustard proved to be the most sensitive crop. Wheat was less sensitive (EC₅₀ = 214.4–243.8 μg a.i./kg soil, for all three pigments), while sunflower and maize were the least sensitive (EC₅₀ = 359.7–417.5 and 456.1–535.8 μg a.i./kg soil, respectively). Field trials were conducted for two years in the Srem region to study the dynamics of clomazone degradation in sandy loam soil. Clomazone was applied pre-plant incorporated (PPI) and post-emergence (POST-EM) in three doses: 480, 720 and 960 g a.i./ha. Soil samples were taken at regular intervals from the day of herbicide application until one year after application and residue concentrations were determined using the white mustard bioassay (based on the measurement of carotenoid content inhibition). The application rate had no consistent effect on the persistence of clomazone. Slower degradation was observed in the PPI treatment than in the POST-EM treatment (8.5 and 15 days longer average half-lives in the first and second year, respectively). Persistence was affected by lower rainfall, resulting in a longer half-life in the second year (12 days on average). Herbicide residues caused no visible injury to white mustard one year after application, while the reduction in carotenoid content ranged from 0.37 to 22.89%, indicating that no injury can occur to any of the tested crops one year after application of clomazone in sandy loam soil. Full article

Microplastic (MP) particles are ubiquitous in the environment and pose a growing threat to ecosystem stability. As concern over their ecological impact increases, biotests and ecotoxicological approaches using plant species have become valuable tools for research. This study aimed to evaluate the effects of varying concentrations of low-density polyethylene (LDPE) MP on seed germination, root development, and shoot growth of white mustard (Sinapis alba L.) under controlled laboratory and pot experiment conditions. For the seven-day laboratory experiment, concentrations of 0.01% and 1% w/w were used, whereas concentrations of 1% and 5% w/w were applied in the ten-day pot experiment. Results indicated no statistically significant effects of LDPE MP on germination rate or germination speed index (GSI) in either setting. However, shoot length data suggest that the 5% LDPE treatment may have a slight stimulatory effect compared to the control, though this trend was marginally significant. These findings highlight the complex and context-dependent nature of MP–plant interactions. Further research is needed to better understand the mechanisms driving these responses and to support the development of mitigation strategies for MP contamination in terrestrial ecosystems. Full article

This paper presents a pilot project conducted by ISPRA and ARPA Campania to develop a monitoring protocol to detect the presence of genetically modified (GM) oilseed rape (Brassica napus) plants resulting from accidental seed dispersal during transportation from entry points to storage and processing facilities; the project has been implemented in Italy’s Campania region. The unintentional dispersal of GM oilseed rape seeds and the potential establishment of feral populations have been identified as environmental concerns in various countries, even when GM oilseed rape is imported solely for processing and not for cultivation. The project activities were designed, taking into account the characteristics of the Italian environment and infrastructures. Multiple sampling campaigns were conducted in autumn 2018, spring 2019, and autumn 2019 to validate the selected transects and assess the presence of Brassicaceae species, with a particular focus on oilseed rape. These efforts involved direct monitoring and sample collection along transport routes from the port of Salerno to seed companies in the provinces of Benevento and Caserta. Field observations and import data revealed a decrease in oilseed rape movement at the port of Salerno in the years preceding the survey, while seed companies near Benevento remained active sites for white mustard (Sinapis alba). The presence of S. alba and the simultaneous occurrence of oilseed rape and Raphanus raphanistrum—a species with high hybridization potential—support the hypothesis that seed companies may act as hotspots for accidental seed dispersal and that potential interspecific gene flow can occur. The study also validated the adopted sampling and molecular analysis methods, including DNA extraction and PCR, for the detection of the Cruciferin A (CruA) gene in all Brassica species collected. These findings highlight the need to strengthen post-marketing monitoring plans, even when GM rapeseed is imported solely for processing, to mitigate the potential risks associated with unintended gene flow. Full article

Objectives: The aim of this double-blind, clinical trial study was to evaluate the clinical and antibacterial effects of a herbal toothpaste containing white mustard Sinapis alba extract in comparison to a placebo toothpaste. Methods: One hundred and thirteen participants with gingivitis were randomly assigned to the test group (experimental herbal toothpaste) or the control group (placebo toothpaste). The plaque index (PI), approximal plaque index (API), gingival index (GI), and bleeding on probing (BoP) were evaluated, and salivary samples for microbial evaluation of the loads of Streptococcus mutans and Lactobacillus spp. were collected at baseline (T₀) and after 4 weeks (T₁). Comparisons were performed between and within groups. Results: A reduction in all periodontal parameters (PI, API, GI, and BoP) was observed. The experimental toothpaste reduced the PI by 2.43, compared to a 1.95 reduction for the placebo (p = 0.041), and BoP by 30.6%, compared to a 26.8% reduction for the placebo (p = 0.037). Statistically significant reductions in salivary S. mutans and Lactobacillus spp. counts were found in the test group. Among patients who used the experimental toothpaste, 19.2% and 9.6% showed counts of S. mutans and Lactobacillus spp., respectively, below 10⁵ CFU/mL, compared to 44.2% and 40.4% in the placebo group. Conclusions: Toothpaste enhanced with white mustard extract was more effective in reducing the PI and BoP indices and decreasing S. mutans and Lactobacillus spp. counts compared to placebo toothpaste. Full article

The noticeable reduction in plant species abundance near industrial hemp (Cannabis sativa L.) highlights the need to investigate its potential allelopathic effects on selected cultivars’ seed germination and seedling growth. Industrial hemp of the “Helena” variety was used to obtain aqueous extracts by conventional (macerate, hydrolate, and post-distillation residue) and green methods (ultrasonic and microwave extracts) in order to treat thirteen most commonly cultivated plant species, including lettuce, kohlrabi, onion, tomato, carrot, pepper, savoy cabbage, rocket, alfalfa, white mustard, pea, sunflower, and parsley. This is the first time that the allelopathic effects of seven different hemp extracts were tested simultaneously on thirteen different species. The extracts were applied at 10, 25, 50, and 100% concentrations. The seed germination percentage and root/shoot length results for all tested plants, except peas, clearly demonstrated an inhibitory effect of higher concentrations of hemp extracts. This effect was observed regardless of variations in chemical composition (CBD, THC, and total polyphenols), suggesting that different extracts have varying impacts on different species. The weakest inhibitory effect on the germination and seedling length for the majority of the tested plant species was noted for PDR, while the strongest inhibitory effect in terms of seedling length was observed in the case of MAE700. Full article

Phytostabilization of metals involves the inactivation of metals in the soil through the use of various materials as soil amendments, which reduces the bioavailability of metals, and then the introduction of vegetation. There are limited data comparing the effectiveness of different phytostabilization amendments under the same soil and environmental conditions. Therefore, the aim of this research was to compare the effectiveness of a range of soil amendments on reducing the extractability of metals, metal uptake by plants, microbial activity in soil and nutrient availability to plants. Eight materials potentially limiting metal availability were used in a pot experiment: two composts (CG, CM), municipal biosolids (SB), bentonite (BEN), phosphorus fertilizer (PF), amorphous iron oxide (FE), waste rock material (WR), calcium carbonate (LM); and these materials were compared with typical fertilization (NPK) and an untreated soil as the control (CTL). The following trace metal-contaminated soils were used in the pot experiment: soil taken from the area of strong dust fall from the zinc and lead smelter (soil P); soil taken from an outcrop of ore-bearing rocks near a smelter waste heap (soil H); soil artificially polluted through smelter dust spill in the 1990s (soil S). In general, the greatest yields of plants (oat and white mustard) were recorded for compost-treated soils. Changes in the solubility of zinc (Zn) and cadmium (Cd) after the application of various amendments largely reflected changes in soil pH. Biosolids caused a significant increase in extractable Zn and Cd, which was related to the decrease in soil pH, while a significant reduction in Cd extractability was observed across soils after the application of both composts, especially the compost characterized by alkaline pH. Interestingly, low extractability of Cd in the soil with the addition of another compost was observed, despite the pH decrease, as compared to the control pots. This fact proves the high sorption capacity of the compost towards Cd. The microbiological analyses revealed the highly beneficial effect of composts for dehydrogenases and nitrification activities, and for soil respiration, whereas soil amendment with iron oxide caused an increase in respiration activity across soils. Full article

The aim of this experiment was to investigate the differences in the uptake and accumulation of rare earth elements (REEs) between selected plant species and the substrates used (soil with increased REE content, ash, and smelter waste). Eight plant species were included in the study: common yarrow (Achillea millefolium), false mayweed (Triplerosperum maritimum), tall fescue (Festuca arundinacea), marigold (Tagetes sp.), maize (Zea mays), white mustard (Sinapis alba), red clover (Trifolium pratense L.), and autumn fern (Dryopteris erythrosora). The study focused on the following REE representatives: lanthanum (La), cerium (Ce), europium (Eu), and gadolinium (Gd). Plant samples, divided into roots and shoots, were analyzed by ICP-MS. The obtained REE concentrations in plant tissues ranged from 9 to 697 µg kg⁻¹ (La), 10 to 1518 µg kg⁻¹ (Ce), 9 to 69 µg kg⁻¹ (Eu), and 9 to 189 µg kg⁻¹ (Gd). To determine the ability of plants to phytoextract REE, two factors were calculated: the translocation factor (TF) and the bioconcentration factor (BCF). The highest TF value was obtained for D. erythrosora growing on a substrate consisting of soil with increased REE content (Gd, TF = 4.03). Additionally, TF > 1 was obtained for all REEs in T. pratense L. In the experiment, the BCF was lower than 1 for all the plants tested. The study provided insight into the varying ability of plants to accumulate REEs, depending on both the plant species and the chemical properties of the substrate. Full article

Light intensity and spectral composition are the main parameters that may be modulated to further affect plant nutritional value and shelf life. The current study aimed to assess how variations in spectral composition and light intensity affect sugar accumulation during the storage of two popular microgreens cultivated in a greenhouse under controlled conditions. Thus, in this study, amaranth (Amaranthus tricolor) and mustard (Brassica juncea) microgreens were grown in a greenhouse at 17/20 ± 3 °C and a 16 h photoperiod was maintained. (I) Four LED light intensities were set: 100, 150, 200, and 250 µmol m⁻² s⁻¹ while using 4000 K white LED lighting. (II) Maintaining 250 µmol m⁻² s⁻¹ the effect of spectrac composition: B_75.6%:R_24.2%:W_0.02%/R_88.9%:B_11.1%/and R_77.6%:W_9.9%:B_3.5% was evaluated. After 10 days from germination, microgreens were harvested and stored in the dark or under white LED light at +4 °C. Samples were collected on D₀, D₁, D₃, and D₅ days of postharvest storage. The results revealed that a wide spectrum of 250 µmol m⁻² s⁻¹ PPFD and R_88.9%:B_11.1% growing conditions produced the highest sugar content, achieving a balance between increased sugar accumulation and reduced deterioration during storage, ultimately extending shelf life. Full article

Insectary plants, such as sweet alyssum, coriander, and white mustard, are well known for their traits that attract beneficial insects, allowing them to protect crops from pests. The aim of the study was to analyze the compounds that are important in the antioxidant response, such as malondialdehyde, ascorbic acid, proline, total phenolics, and total flavonoids, as well as the content of elements, including macroelements (K, Mg, Na, Ca, P, and S) and heavy metals (Cd, Cu, Zn, Pb, Ni, Mn, and Fe) in broad bean plants. These plants were grown in field conditions as the main protected plant alongside a mixture of three insectary plants at different proportions of the individual components. The soil was analyzed in terms of the above-mentioned elements, as well as in terms of its enzymatic activity (arylsulfatase, β-glucosidase, dehydrogenase, FDA (fluorescein diacetate), and acid phosphatase). The introduction of insectary plant mixtures did not cause major changes in the content of the elements in the soil. The changes in the content of elements in broad bean leaves depended on the type of element and the proportion of individual components in the companion plant mixture. However, a general trend of increasing macronutrient content was observed, influenced by the presence of companion plants. All types of companion plant mixtures used enhanced the activity of FDA, while the mixture with 50% sweet alyssum additionally caused an increase in arylsulfatase activity (more than 2 fold). The companion plants improved the physiological condition of the protected plant, which was reflected in the reduced content of proline and total flavonoids. Considering the response of the protected plant to the proposed intercropped plant mixtures and their effect on broad bean growth, it appears that the most suitable mixtures are those with an equal share of all three plant species or a mixture with a predominance of sweet alyssum. Full article

The present investigation deals with comparisons drawn among three types of different mustard seed coat colors, namely, Black (Brassica nigra), Brown (Brassica juncea), and White (Sinapis alba), with respect to protein’s bio-availability through pepsin digestibility, with and without the involvement of major anti-nutritional factors (glucosinolate type AITC, Allylisothiothiocyanate) and relative food functions. These are validated by means of crude protein determination, precipitated protein isolate preparation for evaluating the fat absorption capacity (FAC), emulsifying activity (EA), emulsion stability (ES), whippability, foam stability (FS), the nitrogen solubility index (NSI), and the protein dispersibility index (PDI). The results indicate that the partial removal of glucosinolates from brown mustard (0.91 to 0.31%), black mustard (0.74 to 0.31%), and white mustard (0.58 to 0.30%) improved protein’s digestibility, as analyzed through a pepsin assay, with values of 12.84, 12.60, and 4.53% in brown, black, and white mustard, respectively. Among functional properties, the highest FAC, whippability, foam stability, and NSI values were noted in the brown mustard seed meal, derived from precipitated protein isolates, while EA and PDI were the highest in white mustard seeds, and black seeds possessed the highest ES value. Interestingly, these mustard seed meals are limited in their consumption, albeit by virtue of the different phytochemical and food functional parameters that are being elucidated here. The present research shows the relevance of different food functional properties and the presence of anti-nutritional factors, and uses protein-digestibility tests, which are important deciding parameters for enhanced food consumption in animal diets. Additionally, targeted molecular and protein–protein docking results revealed how and why the mustard seed meals are limited in their consumption by virtue of various metabolite interactions. This thereby opens the gateways to many required in vivo and in silico future research insights among AITC–pepsin, AITC–myrosinase, pepsin–myrosinase, and cruciferin–myrosinase complexes. Consequently, the metabolic pathways governing AITC involved in the glucosinolate–myrosinase system need to be studied in depth for a better understanding of in vivo AITC metabolism. This knowledge can guide future studies in improving the health benefits of mustard seeds and seed meals while addressing their consumption limitations. Full article

Honey is a complex natural nutrient with well-established therapeutic properties recognized in traditional medicine. The purpose of the current work was to compare, in vitro, the bioactive compounds, antioxidants, and antimicrobial properties of 37 honey samples collected from the western region of Algeria and to identify the best sample for potential therapeutic purposes. Estimation of bioactive compounds was carried out by determining the total phenolic and flavonoid contents. Large variability among the samples was observed regarding the total phenolic content (from 24.17 ± 1.38 to 122.15 ± 3.55 mg GAE/100 g honey) and total flavonoid content (from 0.07 ± 0.01 to 33.49 ± 4.90 mg QE/100 g honey). Additionally, antioxidant activity, evaluated by four spectrophotometric assays, displayed fluctuating results among the samples. High positive correlations were observed between β-carotene and DPPH (0.766) and between β-carotene and ABTS (0.600), while inverse correlations were observed between bioactive compounds and antioxidant activity, except for the FRAP method. The antimicrobial activity, determined by well-diffusion assays, exhibited a dose-dependent antibacterial effect, with significant inhibition toward methicillin-resistant Staphylococcus aureus and Pseudomonas aeruginosa reference strains. However, no activity was observed against Candida albicans strains. The MIC and MBC values were identical in most samples (range: 60 to 80% w/v) and predominantly exhibited bactericidal effects. The content of bioactive compounds played a significant role in the antibacterial properties. To summarize, the best honey sample for potential therapeutic purposes corresponded to mild white mustard (S6) and might be used as an alternative in therapeutic applications. Full article

Cigarette butts (CBs) are emerging soil contaminants, releasing chemicals upon contact with moisture. This study examined heavy metal concentrations leached from smoked and unsmoked CBs (Pall Mall, Philip Morris, and Marlboro) into OECD artificial soil and Vertisol soil and their accumulation in white mustard (Sinapis alba L.). Key physiological parameters, including germination rate, plant height, fresh weight, and dry weight, were analyzed, along with the uptake of heavy metals (Al, Fe, Mn, Zn, Ba, Ti, and Cu) and essential elements (Ca, Mg, Na, and K). Results showed that Mn had the highest bioaccumulation index (BAI = 1.10) in OECD soil, while Zn uptake was consistently high across soil types. Soil type significantly influenced plant height (χ² = 41.269, p < 0.01) and elemental composition, with Vertisol soil facilitating greater overall growth and heavy metal uptake than OECD soil. MANOVA revealed no three-way interaction among soil type, CB use, and CB brand on elemental uptake. However, two-way interactions, particularly between soil type and CB use (F (4, 39) = 40.233, p < 0.001, Wilk’Λ = 0.195), showed significant effects on heavy metal uptake. These findings highlight the complex interactions influencing plant contamination, underlining the ecological risks of CB pollution in soils. Full article

Microplants are vegetables, grains and aromatic herbs that are consumed in the stage of young plants, without roots, developed after the germination stage, in the stage of cotyledons and which have a high content of nutrients (antioxidants, vitamins, minerals, fatty acids, lutein, β-carotene, proteins and fibers, etc.), which makes them functional, concentrated foods capable of feeding the world’s ever-growing population. The significant amounts of antioxidants in microgreens have the role of neutralizing free radicals and reducing their harmful impact on human health. The microgreens studied were spinach (Spinacia oleracea) cultivar ‘Lorelay’, mustard (Sinapis alba) cultivar ‘White’ and radish (Raphanus sativus) cultivar ‘Red Rambo’, tested on hemp and coconut substrates and under the influence of the organic biostimulator Biohumussol, based on humic acids. The antioxidant content of the plants was determined by analyzing total carotenoids, lycopene, chlorophyll, β-carotene, polyphenols and flavonoids, as well as the antioxidant activity by ABTS and DPPH methods. The obtained results indicated that the reaction of the plant material depends on the composition of the substrate and the presence of the applied biostimulator. The highest contents of substances with an antioxidant role were obtained from the microgreens on the hemp substrate, especially mustard and radishes, and the biostimulator proved to be compatible with the spinach microgreens. Full article

Microgreens are vegetable greens that are harvested early while they are still immature and have just developed cotyledons. One of the disadvantages and a challenge in production is that they exhibit a short shelf life and may be damaged easily. In seeking to prolong the shelf life, some pre- and postharvest interventions have been investigated. Here, kale and mustard microgreens were grown in a controlled-environment walk-in chamber at +21/17 °C, with ~65% relative air humidity, while maintaining the spectral composition of deep red 61%, blue 20%, white 15%, and far red 4% (150, 200, and 250 µmol m⁻² s⁻¹ photosynthetic photon flux density (PPFD)). Both microgreens seemed to exhibit specific and species-dependent responses. Higher PPFD during growth and storage in light conditions resulted in increased contents of TPC in both microgreens on D₅. Additionally, 150 and 250 PPFD irradiation affected the α-tocopherol content by increasing it during postharvest storage in kale. On D₀ 150 for kale and 200 PPFD for mustard microgreens, β-carotene content increased. D₅ for kale showed insignificant differences, while mustard responded with the highest β-carotene content, under 150 PPFD. Our findings suggest that both microgreens show beneficial outcomes when stored in light compared to dark and that mild photostress is a promising tool for nutritional value improvement and shelf-life prolongation. Full article