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29 pages, 11629 KB  
Article
Spatiotemporal Modeling of Mangrove Carbon Stock Along Pakistan’s Coast Using Multi-Sensor Sentinel and Landsat Data
by Junaid Ahmad Qadri, Asif Sajjad and Aqib Hassan Ali Khan
Sensors 2026, 26(13), 4117; https://doi.org/10.3390/s26134117 (registering DOI) - 29 Jun 2026
Abstract
This study quantifies coastal mangrove carbon stocks and their interannual variability along the Pakistan coastline by developing a multi-sensor fusion framework integrated with a process-based light use efficiency (LUE) modeling approach. To ensure high-cadence monitoring and overcome persistent cloud cover over the Indus [...] Read more.
This study quantifies coastal mangrove carbon stocks and their interannual variability along the Pakistan coastline by developing a multi-sensor fusion framework integrated with a process-based light use efficiency (LUE) modeling approach. To ensure high-cadence monitoring and overcome persistent cloud cover over the Indus Delta, data from multiple satellite sensors including Landsat 8/9 and Sentinel-2 within Google Earth Engine were utilized. Sentinel-2-derived Normalized Difference Vegetation Index (NDVI) data composited for the January–March period was processed to estimate vegetation productivity. Field-based validation of biomass estimates was conducted using 57 georeferenced sampling points, cross-compared with Sentinel-2 data. Mangrove extent was delineated through land use and land cover (LULC) classification into water bodies, mangroves, mudflats, land parcels, and sand surfaces. The LUE model incorporated environmental stress scalars, including temperature, vapor pressure deficit (VPD), salinity, and photosynthetically active radiation (PAR) to estimate gross primary productivity and derive total biomass, which was subsequently converted into carbon stocks. Results indicate a mean carbon stock of 31.95 Mg C ha−1 (equivalent to 117.3 Mg CO2 ha−1), with significant interannual variation (coefficient of variation = 19.8%). A significant decline in carbon stocks was observed in 2021 (−11.11%; 3.56 Mg C ha−1), corresponding to a reduction in NDVI value (0.55 compared to 0.58 in other years). Spatial analysis revealed substantial heterogeneity in carbon distribution (20.51 to 55.93 Mg C ha−1), primarily influenced by localized salinity gradients and water stress conditions. This study mapped mangrove extent, quantified environmental stress, and estimated carbon stocks across Pakistan’s coast from 2020 to 2024, delivering a spatially resolved, multi-year baseline for coastal carbon assessment and ecosystem monitoring in arid tidal environments. Full article
(This article belongs to the Special Issue Optical Sensing for Environmental Monitoring—2nd Edition)
20 pages, 4042 KB  
Article
Dynamic Safety Boundary Modeling and Flexibility Assessment of Alkaline Electrolyzers Under Fluctuating Wind and Solar Conditions
by Siyuan Zhang, Yang Li, Xiaoyan Zhao, Ting Tang, Yonghua Chen and Jingang Wang
Appl. Sci. 2026, 16(13), 6477; https://doi.org/10.3390/app16136477 (registering DOI) - 29 Jun 2026
Abstract
Alkaline water electrolysis (ALK) is essential for renewable energy integration, yet traditional models using a fixed minimum operating power often overestimate low-load flexibility by neglecting state-dependent safety boundaries. This study develops an electro-thermal-mass multiphysics dynamic model that treats the transient hydrogen content in [...] Read more.
Alkaline water electrolysis (ALK) is essential for renewable energy integration, yet traditional models using a fixed minimum operating power often overestimate low-load flexibility by neglecting state-dependent safety boundaries. This study develops an electro-thermal-mass multiphysics dynamic model that treats the transient hydrogen content in oxygen (H2-in-O2) concentration as a first-principles state variable. Based on a quasi-steady-state safety balance, a dynamic minimum operating power constraint is derived to replace empirical static limits. A key feature of this model is the explicit coupling of Arrhenius thermal diffusion and pressure-differential-driven permeation during load transients, allowing the safety threshold to respond to real-time system states. Year-round simulations of a 30 MW industrial system under a wind–solar time series reveal that thermal inertia, with a time constant of approximately 4.2 h, induces a sustained mismatch between low-power operation and high system temperature. Under high-temperature and rapid-ramp conditions, the dynamic safety lower bound reaches 28.2% of the rated capacity, exceeding the conventional 20% static threshold by 8.2 percentage points. This deviation results in 378.3 MWh of implicit curtailment and 60.5 h of additional downtime annually, leading to a green hydrogen production deficit of approximately 42.2 t/year. This research provides a theoretical foundation and technical reference for the optimal control and flexibility assessment of industrial-scale green hydrogen systems under fluctuating power supply conditions. Full article
(This article belongs to the Section Energy Science and Technology)
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30 pages, 1483 KB  
Article
Impact of Biochar and Its Modification on Heavy Metals and Drought in Rice: Knowns, Unknowns, and Research Directions
by Bilal Zulfiqar, Rui Chen, Qiufen Feng, Chao He, Yuxiao Sun, Yang Zhang, Yanan Wang, Xibai Zeng, Cuixia Wu and Nan Zhang
Agronomy 2026, 16(13), 1254; https://doi.org/10.3390/agronomy16131254 (registering DOI) - 29 Jun 2026
Abstract
Rice, a staple food for over half of the global population, faces significant threats from environmental stressors such as heavy metal (HMs) contamination, notably cadmium (Cd) and arsenic (As), and increasing drought severity, exacerbated by climate change. These challenges not only compromise rice [...] Read more.
Rice, a staple food for over half of the global population, faces significant threats from environmental stressors such as heavy metal (HMs) contamination, notably cadmium (Cd) and arsenic (As), and increasing drought severity, exacerbated by climate change. These challenges not only compromise rice yield and quality but also pose serious food safety risks due to HM accumulation in grains, endangering human health. Modified biochar (MBC), a carbon-rich material derived from the pyrolysis of organic matter with post-treatment enhancements, has emerged as a strategy to address these dual stressors. MBC application (typically 5–20 t ha−1) reduces Cd and As bioavailability in paddy soils by 40–60% and decreases metal accumulation in rice grains by 20–85% compared to the control. Under drought conditions, MBC improves soil water-holding capacity by 11–45% and enhances crop water use efficiency by 15–24%, leading to yield improvements of 20–50% under moderate water deficit. Furthermore, MBC supports nutrient availability, fosters robust root systems, and enhances soil aeration, collectively improving rice growth under adverse conditions. Beyond its agronomic benefits, MBC provides a framework for addressing multiple challenges by integrating scientific innovation, policy alignment, and community participation. This approach not only reduces heavy metal toxicity and strengthens plant resilience but also enhances food security and advances Sustainable Development Goals (SDGs 2, 3, 4, 12, 13, 15, 17). By promoting environmentally sustainable agriculture and contributing to climate change mitigation, MBC represents a transformative tool for ensuring sustainable rice production in the face of global challenges. Full article
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17 pages, 4415 KB  
Article
Sea-Level Fall over Rainfall: Mask-Applied Satellite Reassessment of Gulf of Carpentaria Mangrove Dieback
by Seung-Jun Lee, Jisung Kim, In-Seok Heo and Hong-Sik Yun
Sustainability 2026, 18(13), 6562; https://doi.org/10.3390/su18136562 (registering DOI) - 29 Jun 2026
Abstract
Mangrove forests deliver globally significant climate-mitigation and coastal-protection benefits, yet their resilience to climate extremes remains poorly quantified—a key uncertainty for sustainable coastal management. We reassess the unprecedented 2015–2016 mangrove dieback along ~1000 km of the Gulf of Carpentaria, northern Australia, to determine [...] Read more.
Mangrove forests deliver globally significant climate-mitigation and coastal-protection benefits, yet their resilience to climate extremes remains poorly quantified—a key uncertainty for sustainable coastal management. We reassess the unprecedented 2015–2016 mangrove dieback along ~1000 km of the Gulf of Carpentaria, northern Australia, to determine its driver and whether the collapse was structurally abrupt. Combining a mangrove-extent mask, an 11-year radar backscatter series, satellite precipitation, the modeled sea level, the reanalysis temperature and atmospheric dryness, and an El Niño index, we show that an apparent abrupt radar decline during the event was an artifact of non-vegetated tidal-flat and open-water pixels: once analysis was restricted to mangrove pixels, the signal remained stable throughout. Independent spaceborne lidar confirmed that canopy structure concentrates within the mapped mangrove zones, validating the mask. The dieback coincided with a strong sea-level fall, with anomalies reaching about −15 cm, under near-to-above-average rainfall and low atmospheric dryness, indicating that sea-level fall, not rainfall deficit, was the proximate stressor. These findings advance sustainable, mask-applied satellite monitoring of blue-carbon ecosystems and provide an evidence base for climate-adaptive coastal-resilience planning under intensifying climate variability. Full article
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27 pages, 9178 KB  
Article
Mulberroside A Alleviates Scopolamine-Induced Cognitive Deficits by Suppressing Neuroinflammation and Oxidative Stress via the Dubosiella-Associated Microbiota–Gut–Brain Axis
by Jin Li, Shirui Cheng, Wenqi Zhang, Shourong Qiao, Luzhi Zhang, Mengxu Yao, Yunxia Zhang, Biao Wang and Changjing Wu
Biology 2026, 15(13), 1030; https://doi.org/10.3390/biology15131030 (registering DOI) - 28 Jun 2026
Abstract
Mulberroside A (MsA) possesses neuroprotective effects, but whether it alleviates Alzheimer’s disease (AD)-like cognitive impairment through the microbiota–gut–brain axis remains unclear. Using a scopolamine-induced mouse model of acute cognitive impairment (male ICR mice, n = 10/group), we demonstrated that daily administration of MsA [...] Read more.
Mulberroside A (MsA) possesses neuroprotective effects, but whether it alleviates Alzheimer’s disease (AD)-like cognitive impairment through the microbiota–gut–brain axis remains unclear. Using a scopolamine-induced mouse model of acute cognitive impairment (male ICR mice, n = 10/group), we demonstrated that daily administration of MsA (10, 20, and 30 mg/kg/day) for 5 weeks significantly ameliorated cognitive performance in novel object recognition and Morris water maze tests. At the optimal dose (30 mg/kg/day), MsA suppressed hippocampal microglial activation, reduced pro-inflammatory cytokines (IL-6, IL-1β, TNF-α), and attenuated oxidative stress by decreasing malondialdehyde (MDA) while restoring superoxide dismutase (SOD) and glutathione (GSH) levels. MsA also strengthened intestinal barrier integrity (ZO-1, occludin) and significantly altered the gut microbiota, notably increasing the beneficial genus Dubosiella. Brain metabolomics indicated that MsA reversed scopolamine-induced metabolic disturbances, mainly restoring phospholipid balance. Correlation analysis demonstrated a strong gut–brain connection, with Dubosiella abundance positively associated with neuroprotective phospholipids and negatively with stress markers. Furthermore, fecal microbiota transplantation from MsA-treated donors successfully replicated these behavioral improvements in recipient mice, underscoring the functional involvement of the reshaped microbiome rather than a simple autonomous recovery. These results suggest that MsA alleviates AD-like cognitive impairment by reducing neuroinflammation and oxidative stress through microbiota remodeling, enhancing the intestinal barrier, and modulating the Dubosiella-associated gut–metabolite–brain axis, making MsA a promising multi-target nutraceutical for ameliorating AD-like cognitive deficits. Full article
(This article belongs to the Section Neuroscience)
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16 pages, 778 KB  
Systematic Review
Research Advances and Emerging Challenges in Various Types of Drought Monitoring: An Integrative Review
by Haichao Yu, Sien Li, Yang Zhang, Jiaming Zhang, Jiajin Ding and Shengwen Liu
Agronomy 2026, 16(13), 1248; https://doi.org/10.3390/agronomy16131248 (registering DOI) - 27 Jun 2026
Viewed by 96
Abstract
Drought is one of the most complex and impactful natural hazards under global climate change, exerting profound effects on water resources, agricultural productivity, ecosystem stability, and socio-economic systems. Despite extensive research, current drought studies remain fragmented due to inconsistent definitions, index-specific monitoring approaches, [...] Read more.
Drought is one of the most complex and impactful natural hazards under global climate change, exerting profound effects on water resources, agricultural productivity, ecosystem stability, and socio-economic systems. Despite extensive research, current drought studies remain fragmented due to inconsistent definitions, index-specific monitoring approaches, and limited understanding of cross-variable and cross-scale interactions. The objective of this review is to synthesize recent advances in drought monitoring and to establish an integrated understanding of drought as a coupled, multiscale process. We revisit traditional drought typologies, including meteorological, agricultural, hydrological, groundwater drought, and socio-economic drought, and critically evaluate their commonly used monitoring indices and data sources. We highlight that no single indicator can adequately capture the full dynamics of drought evolution, emphasizing the need for multi-index integration and process-based monitoring frameworks. Moreover, we examine the mechanisms of drought propagation, demonstrating that drought evolves through nonlinear and scale-dependent pathways linking atmospheric conditions, soil moisture, hydrological processes, and human water use. In particular, the emergence of flash drought reveals a shift from conventional water-deficit-driven processes to multi-process coupled dynamics, posing new challenges for early warning and prediction. Furthermore, we discuss how climate change and human activities jointly reshape drought characteristics by altering hydrological cycles, land–atmosphere interactions, and water resource management systems. The review reveals three major findings. First, drought monitoring is progressively shifting from single-index assessments toward integrated, multi-source monitoring frameworks. Second, drought propagation is inherently nonlinear and scale-dependent, involving complex interactions among climatic, hydrological, ecological, and human systems. Third, flash drought and groundwater drought have emerged as critical research frontiers due to their rapid evolution, monitoring challenges, and increasing impacts under climate change. Finally, we identify key challenges in drought research, including methodological uncertainties, data limitations, and the lack of a unified theoretical framework. These findings support a paradigm shift from traditional drought classification toward an integrated process-based perspective and provide guidance for the development of next-generation drought monitoring and early-warning systems. Full article
16 pages, 1224 KB  
Article
Association Between Contrasting Water Regimes and Telomere Length Variation in Field-Grown Grapevines: An Integrated Physiological, Metabolomic and Molecular Approach
by Alessandra Iannuzzi, Ramona Pistucci, Arturo Erbaggio, Rossella Albrizio, Andrea Vitale, Filippo Accomando, Maurizio Buonanno, Antonio Dario Troise, Sabrina De Pascale and Antonello Bonfante
Plants 2026, 15(13), 1988; https://doi.org/10.3390/plants15131988 (registering DOI) - 26 Jun 2026
Viewed by 114
Abstract
Climate change is increasing the exposure of crops to drought stress, highlighting the need for integrative approaches to assess plant responses under field conditions. In this study, telomere length (TL) was evaluated in field-grown grapevine (Vitis vinifera L., cv. Aglianico) subjected to [...] Read more.
Climate change is increasing the exposure of crops to drought stress, highlighting the need for integrative approaches to assess plant responses under field conditions. In this study, telomere length (TL) was evaluated in field-grown grapevine (Vitis vinifera L., cv. Aglianico) subjected to rainfed (RF) and controlled deficit irrigation (CDI) regimes. A qPCR-based protocol was applied together with physiological measurements, UAV-derived vegetation indices, and berry metabolomic profiling to investigate plant responses to different water regimes. Physiological and metabolomic analyses confirmed distinct responses between treatments, with rainfed vines showing more negative leaf water potential, lower stomatal conductance, and increased accumulation of stress-associated metabolites, including anthocyanins and abscisic acid. Linear mixed-effects modeling showed no significant difference in TL between treatments at the beginning of the experimental period (p = 0.198), whereas rainfed vines displayed significantly lower TL values than irrigated vines at the end of the growing season (p = 0.0009). TL decreased significantly over time in both treatments. The treatment × time interaction suggested a greater TL reduction in rainfed vines in the primary model (p = 0.064), and this effect was significant in a complete-pair sensitivity analysis (p = 0.036). These findings indicate an association between irrigation regime and telomere length variation under field conditions. The study provides preliminary evidence supporting the potential application of TL measurements for investigating plant responses to environmental stress in grapevine. Full article
(This article belongs to the Special Issue Grapevine Response to Abiotic Stress, 2nd Edition)
18 pages, 1186 KB  
Article
Potato Tuberisation Responses to Drought and a Film-Forming Antitranspirant
by Oluwatoyin Favour Olu-Olusegun, Aidan Farrell, James Monaghan and Peter Kettlewell
Plants 2026, 15(13), 1971; https://doi.org/10.3390/plants15131971 - 26 Jun 2026
Viewed by 130
Abstract
Film-forming antitranspirants may help potatoes tolerate moderate drought, but their effects on early tuberisation and tuber size distribution remain unclear. Two pot experiments were conducted in a polytunnel (late summer) and a glasshouse (winter–spring), with moderate drought imposed during tuber initiation and early [...] Read more.
Film-forming antitranspirants may help potatoes tolerate moderate drought, but their effects on early tuberisation and tuber size distribution remain unclear. Two pot experiments were conducted in a polytunnel (late summer) and a glasshouse (winter–spring), with moderate drought imposed during tuber initiation and early bulking, alone (DT) or combined with an antitranspirant (di-1-p-menthene; VGDT). Leaf relative water content (RWC), stolon traits, and tuber yield and size distribution were measured. Moderate drought reduced RWC, stolon number, and tuber set, which indicates the sensitivity of early tuber development to water deficit. VGDT increased leaf RWC under drought from 55% to 71% in Experiment 1 and from 62% to 73% in Experiment 2, while the total tuber number under moderate drought increased from 5.2 to 11.7 tubers plant−1 in Experiment 1 and from 6.1 to 10.7 tubers plant−1 in Experiment 2. VGDT also increased the number of large (≥9 cm) tubers, shifting size distribution towards marketable classes. Although Vapor Gard improved plant water status and tuber number under drought, it did not restore performance to irrigated levels. These findings indicate its value as a complementary tool to mitigate drought-related losses during tuberisation, not a substitute for irrigation. Full article
(This article belongs to the Section Plant Response to Abiotic Stress and Climate Change)
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28 pages, 3896 KB  
Article
Investment Gap and Environmental Goals in Spain’s Urban Water Cycle
by César Sánchez-Pérez, María-Inmaculada López-Ortiz and Patricia Fernández-Aracil
Water 2026, 18(13), 1566; https://doi.org/10.3390/w18131566 - 26 Jun 2026
Viewed by 356
Abstract
Spain is among the European countries facing the highest water stress, notably along the Mediterranean arc. Despite this, it possesses unique capabilities in water management; it leads Europe in water reuse and desalination technologies, and has the continent’s highest number of dams per [...] Read more.
Spain is among the European countries facing the highest water stress, notably along the Mediterranean arc. Despite this, it possesses unique capabilities in water management; it leads Europe in water reuse and desalination technologies, and has the continent’s highest number of dams per capita, securing most of its urban water supply. Nonetheless, the investment gap in the urban water cycle challenges Spain’s ability to meet European Union environmental targets and ensure the sustainability of public health and economic activities. Therefore, this study analyzes the causes of Spain’s urban water investment deficit, arguing that these challenges stem from institutional factors rather than a lack of resources or technological development. The research identifies three primary governance failures: the lack of a national governing body to harmonize water policies, a fragmented pricing system that drives the infrastructure gap, and a regulatory framework that restricts private sector involvement in managing non-conventional water resources. Consequently, this study highlights the urgent need for adaptive governance to deploy all available tools to respond to the specific needs of each territory in scenarios of uncertainty and climate change. Full article
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18 pages, 4191 KB  
Article
Deficit Irrigation Strategies Modulate Grape Maturation and Volatile Aroma Composition in Pinot Gris Wines
by Mirko Sodini, Alessandro Pichierri, Lara Tat, Piergiorgio Comuzzo, Amelia Caffarra, Selena Tomada, Klemen Lisjak, Andreja Vanzo and Paolo Sivilotti
Agronomy 2026, 16(13), 1232; https://doi.org/10.3390/agronomy16131232 - 25 Jun 2026
Viewed by 183
Abstract
Climate change is increasing the frequency of droughts, raising the need for sustainable irrigation management in viticulture. This study evaluated the effects of three deficit irrigation regimes—well-watered (WW), mild deficit (MiD), and moderate deficit (MoD)—implemented through the decision support system Vintel® in [...] Read more.
Climate change is increasing the frequency of droughts, raising the need for sustainable irrigation management in viticulture. This study evaluated the effects of three deficit irrigation regimes—well-watered (WW), mild deficit (MiD), and moderate deficit (MoD)—implemented through the decision support system Vintel® in a Pinot gris vineyard in Friuli Venezia Giulia (Italy). Vine physiology, grape ripening, and wine aroma profile were assessed across two seasons. The water deficit treatments modulated yield parameters (specifically, cluster weight was reduced by 12% and 10% for Mid and Mod as compared to WW) and delayed sugar accumulation, particularly under MoD (9% Brix reduction as compared to WW). While basic wine composition largely reflected grape maturity, volatile aroma compounds showed variable responses to irrigation and were strongly modulated by seasonal conditions. MiD had a minimal impact on the aroma profile, whereas MoD led to reduced sugar and altered volatile composition, especially under hot and dry conditions. DSS-based mild-deficit irrigation can be adopted to reduce vineyard water consumption without compromising Pinot gris wine quality. Full article
(This article belongs to the Section Water Use and Irrigation)
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23 pages, 7989 KB  
Article
Second-Year Effects of Biochar, Biosolids, and Greenwaste on Tall Fescue Under Deficit Irrigation: Part II
by Jaime Barros Silva Filho, Jonathan Montgomery, Ray G. Anderson and Milton E. McGiffen
Agronomy 2026, 16(13), 1230; https://doi.org/10.3390/agronomy16131230 - 25 Jun 2026
Viewed by 192
Abstract
Soil amendments are widely applied for water conservation in urban turfgrass, yet whether establishment-phase benefits persist into a mature-stand remains unclear. This study evaluated biochar, biosolids, and greenwaste on tall fescue (Schedonorus arundinaceus) over a 108-day mature-stand trial under deficit (50% [...] Read more.
Soil amendments are widely applied for water conservation in urban turfgrass, yet whether establishment-phase benefits persist into a mature-stand remains unclear. This study evaluated biochar, biosolids, and greenwaste on tall fescue (Schedonorus arundinaceus) over a 108-day mature-stand trial under deficit (50% ET0) and moderate (85% ET0) irrigation, both below full replacement. Canopy performance was assessed by visual quality and NDVI, with van Genuchten soil-water retention modeling. Unlike the establishment-phase advantages reported for the organic amendments in Part I, the second-year results reversed sharply: moderate biochar (12.36 t ha−1) was most hydraulically stable, holding the highest plant-available water (PAW ≈ 0.18 cm3 cm−3, above the control and organic amendments) and the most stable canopy. High-rate biochar (24.71 t ha−1) underperformed the control under deficit irrigation, indicating constraints beyond water retention at the highest rate. Greenwaste and biosolids raised volumetric water content but provided lower PAW than moderate biochar. For greenwaste, a reduced field capacity offset this; for biosolids, an elevated permanent wilting point limited the extractable fraction. Biosolids failed to maintain acceptable quality even under the 85% ET0. Because first-year success does not guarantee mature-stand resilience, amendment stability and rate optimization, rather than application volume, emerge as long-term management priorities under water-limited conditions. Full article
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24 pages, 1939 KB  
Article
The Wheat Nitro-Proteome: Protein Nitration Profiles During Drought and Rehydration
by Marta Gietler, Justyna Fidler-Jarkowska and Małgorzata Nykiel
Plants 2026, 15(13), 1951; https://doi.org/10.3390/plants15131951 - 24 Jun 2026
Viewed by 177
Abstract
Protein nitration within the nitro-proteome is a dynamic component of drought and recovery responses in wheat (Triticum aestivum L.), yet its role in stress adaptation remains unclear. Young wheat seedlings demonstrate a degree of drought resistance, characterized by physiological and morphological adaptations, [...] Read more.
Protein nitration within the nitro-proteome is a dynamic component of drought and recovery responses in wheat (Triticum aestivum L.), yet its role in stress adaptation remains unclear. Young wheat seedlings demonstrate a degree of drought resistance, characterized by physiological and morphological adaptations, during the initial growth phases. However, this tolerance begins to diminish significantly in 5-day-old seedlings. The mechanisms behind this phenomenon are unclear. Our results indicate that it may be related to protein nitration. This study compared the physiological and nitrosative responses of 4-day-old drought-tolerant and 6-day-old sensitive wheat seedlings subjected to drought followed by rehydration. In tolerant seedlings, in contrast to sensitive ones, the water saturation deficit after rehydration returned to the control levels, confirming their drought tolerance. Moreover, NO2 accumulation in the recovery group was significantly higher in sensitive seedlings than in the control group. Results indicate that drought resistance correlates with protein nitration during the recovery phase. Nitro-proteomic analysis revealed that in tolerant seedlings, protein nitration is limited. The most significant differences are observed in the recovery group, with predominant downregulation of protein nitration in tolerant seedlings and significant upregulation of numerous proteins in sensitive seedlings. Upregulated nitration of vital proteins involved in energy production, photosynthesis (such as the Rubisco large subunit), ATP synthases, and cytosolic malate dehydrogenase may lead to disturbances in energy metabolism and thus prevent an effective response to changing environmental conditions. These findings suggest that regulation of protein nitration during recovery may contribute to drought resilience in wheat and could represent a potential target for improving stress tolerance. Full article
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24 pages, 5266 KB  
Article
Prediction of Groundwater-Level Fluctuations Under Climate Change Conditions in the Berrechid Plain (Morocco) Using a Hybrid Physical–Machine Learning Approach
by Adil Zerouali, Mohamed Jalal El Hamidi, Abdelkader Larabi, Mohamed Faouzi and Omar Chafik
Hydrology 2026, 13(7), 166; https://doi.org/10.3390/hydrology13070166 - 24 Jun 2026
Viewed by 132
Abstract
The issue of water resources in a semi-arid country such as Morocco has been present for many years and is becoming increasingly critical. The droughts experienced over recent decades have demonstrated the country’s extreme vulnerability to any water deficit. In this context, the [...] Read more.
The issue of water resources in a semi-arid country such as Morocco has been present for many years and is becoming increasingly critical. The droughts experienced over recent decades have demonstrated the country’s extreme vulnerability to any water deficit. In this context, the Berrechid plain represents a relevant case study illustrating both the practical and theoretical challenges of groundwater governance. The aquifer is heavily exploited to satisfy agricultural, industrial, and domestic needs. This study develops a hybrid “grey-box” modeling approach for predicting groundwater depth (GWD) fluctuations under climate change (CC). Unlike conventional black-box machine learning models, our framework combines a deterministic physical engine with a stochastic machine learning corrector. The physical component simulates aquifer mass balance using the Hargreaves method for evapotranspiration, linear drainage, climate memory via exponential decay, and an anthropogenic trend parameter (xi). The machine learning component—XGBoost with quantile regression—is trained exclusively on physical model residuals and predicts the 5th, 50th, and 95th percentiles, providing explicit 90% confidence intervals. Hydrological states (dry, normal, wet) are identified via K-means clustering for context-aware correction. The model is calibrated using historical data (1972–2019) and validated using blocked time-series cross-validation. Climate projections under the RCP 4.5 and RCP 8.5 scenarios were used to forecast GWD up to 2100. At piezometer 3933/20, the best performance was achieved, with an RMSE of 0.347 m and a KGE of 0.742 during the validation period. The proposed approach is suitable for seasonal GWD forecasting and offers practical value for water managers and decision-makers in the Berrechid region. Full article
15 pages, 1243 KB  
Article
The Bioactive Compounds and Antioxidant Capacity of Nopal Cladodes (Opuntia spp.) as Influenced by Irrigation
by Edén A. Luna-Zapién, Jorge A. Zegbe, Andrea de J. Campos-Badillo, Jolanta E. Marszalek, Juan R. Esparza-Rivera and Jorge A. Meza-Velázquez
Antioxidants 2026, 15(7), 787; https://doi.org/10.3390/antiox15070787 - 24 Jun 2026
Viewed by 207
Abstract
The prickly pear is a crop of socioeconomic relevance in arid regions, and its productivity and chemical composition depend on water availability. The effect of irrigation on the crop’s biochemical quality was evaluated. Cladodes of cultivars: ‘Amarilla Olorosa’, ‘Cristalina’, ‘Dalia Roja’, and ‘Roja [...] Read more.
The prickly pear is a crop of socioeconomic relevance in arid regions, and its productivity and chemical composition depend on water availability. The effect of irrigation on the crop’s biochemical quality was evaluated. Cladodes of cultivars: ‘Amarilla Olorosa’, ‘Cristalina’, ‘Dalia Roja’, and ‘Roja Lisa’, were subjected to three treatments: no irrigation (NI), supplemental irrigation (SI), equivalent to 50% of the crop’s evapotranspiration, and full irrigation (FI). Subsequently, cladodes were collected, and total polyphenols and flavonoids, polyphenol profile, and antioxidant capacity were determined. Cladodes under NI had the highest concentrations of flavonoids, although the lowest values of total polyphenols. In the cladode extracts, myricetin, rutin, catechin, as well as caffeic, chlorogenic, dihydroxybenzoic, and vanillic acids were identified. Overall, cladodes grown under FI and SI showed higher levels of phenolic acids (caffeic, chlorogenic, and vanillic), while concentrations of catechin, myricetin, and rutin were higher under SI and NI. Antioxidant capacity was higher in NI cladodes assessed by ABTS and DPPH, while the FRAP assay showed higher values under SI. Among the cultivars, ‘Amarilla Olorosa’ stood out for its high content of bioactive compounds, confirming the potential of nopal cladodes as a source of antioxidant metabolites with agro-industrial applications. Full article
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16 pages, 2372 KB  
Article
Selenium Biofortification Improves Grain Quality and Reduces Arsenic Accumulation in Rice Under Alternate Wetting and Drying Irrigation
by María J. Poblaciones, Luis Vicente, Damián Fernández-Rodríguez, Ángel Albarrán, David Peña and Antonio López-Piñeiro
Agronomy 2026, 16(13), 1220; https://doi.org/10.3390/agronomy16131220 - 24 Jun 2026
Viewed by 184
Abstract
Rice production is under increasing threat from adverse climatic trends that exacerbate water scarcity and compromise food safety. The need to transition toward water-saving irrigation is urgent, as is the requirement of addressing the dual burden of selenium (Se) deficiency and arsenic (As) [...] Read more.
Rice production is under increasing threat from adverse climatic trends that exacerbate water scarcity and compromise food safety. The need to transition toward water-saving irrigation is urgent, as is the requirement of addressing the dual burden of selenium (Se) deficiency and arsenic (As) toxicity. This 3-year field study (2020–2022) is the first to evaluate the effects of integrated water-saving irrigation. Permanent flood irrigation (Flood) or alternate wetting and drying was used, in which fields were reflooded when the soil matric potential reached −20 kPa (Reflood-20) and −70 kPa (Reflood-70); the effects of foliar Se biofortification at 15 g Se ha−1 with sodium selenate (15-Se) or no Se (No-Se) on rice production and Se and As accumulation were also investigated. The results identified the Reflood-20 regime as the optimal strategy, achieving 36% water savings without significant grain yield penalties while enhancing grain quality. Foliar Se application successfully increased the dehulled grain Se content by 10.7-fold, effectively meeting human dietary requirements. The As contents were decreased by 27.6% due to water restriction, and an additional 10% loss was observed because of Se supplementation. Analysis of the straw also showed a 23.5% decrease in As and a 5.7-fold increase in Se. Consequently, the synergy between moderate deficit irrigation and Se biofortification provides a robust, cost-effective framework for the large-scale production of safer, nutrient-dense rice, reconciling resource efficiency with food security. Full article
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