Search Results (860)

Regenerative endodontics seeks to restore the vascularized pulp–dentin complex following conventional root canal therapy, yet reliable neovascularization within the constrained root canal remains a key challenge. This study investigates the development of an injectable, dual-curing hydrogel based on methacrylated decellularized amniotic membrane (dAM-MA) and compares its performance to a conventional gelatin methacryloyl (GelMA). The dAM-MA platform was designed for biphasic release, incorporating both free vascular endothelial growth factor (VEGF) for an initial burst and matrix-metalloproteinase-cleavable VEGF conjugates for sustained delivery. The dAM-MA hydrogel achieved shape-fidelity via thermal gelation at 37 °C and possessed tunable stiffness (0.5–7.8 kPa) after visible-light irradiation. While showing high cytocompatibility comparable to GelMA (>125% hDPSC viability), the dAM-MA platform markedly outperformed the control in promoting endothelial tube formation (up to 800 µm total length; 42 branch points at 96 h). The biphasic VEGF release from dAM-MA matched physiological injury kinetics, driving both early chemotaxis and late vessel maturation. These results demonstrate that dAM-MA hydrogels combine native extracellular matrix complexity with practical, dual-curing injectability and programmable VEGF kinetics, offering a promising scaffold for minimally invasive pulp–dentin regeneration. Full article

With the development of wave energy, a promising renewable resource, oscillating water column (OWC) devices, has been extensively studied for its potential in harnessing this energy. However, traditional OWC devices face challenges such as corrosion and damage from prolonged exposure to harsh marine environments, limiting their long-term viability and efficiency. To address these limitations, this paper proposes a novel U-tube type dual chamber OWC wave energy conversion device integrated within a marine vehicle. The research involves the design of a U-tube dual-chamber OWC device, which utilizes the pitch motion of a marine vehicle to drive the oscillation of water columns within the U-tube, generating reciprocating airflow that drives an air turbine. Numerical simulations using computational fluid dynamics (CFD) were conducted to analyze the effects of various structural dimensions, including device length, width, air chamber height, U-tube channel width, and bottom channel height, on the aerodynamic power output. The simulations considered real sea conditions, focusing on low-frequency waves prevalent in China’s sea areas. Simulation results reveal that increasing the device’s length and width substantially boosts aerodynamic power, while air chamber height and U-tube channel width have minor effects. These findings provide valuable insights into the optimal design of U-tube dual-chamber OWC devices for efficient wave energy conversion, laying the foundation for future physical prototype development and experimental validation. Full article

In the attention-driven environment of online video platforms, understanding the factors that influence content selection and channel subscriptions is crucial for creators, marketers, and platform managers. This study investigates how thumbnails, view counts, video length, genre, and the number of advertisements affect user decision-making on YouTube. Grounded in random utility theory and reference-dependent preference theory, this study conducted a choice experiment with 525 respondents and employed a combined model of rank-ordered and binary logit methods to analyze viewing and subscription behaviors. The results indicate a significant preference for thumbnails with subtitles and shorter videos. Notably, we found evidence of reference-dependent effects, whereby a higher-than-expected number of ads decreased viewing probability, while a lower-than-expected number significantly increased subscription probability. This study advances our understanding of the factors that influence user behavior on social media, specifically in terms of viewing and subscribing, and empirically supports prospect theory in the online advertising market. Our findings offer both theoretical and practical insights into optimizing video content and monetization strategies in competitive social media markets. Full article

Addressing the challenges of bulky, low-efficiency sound-insulation materials at low frequencies, this work proposes an acoustic metamaterial based on curve fractal channels. Each unit cell comprises a concentric circular-ring channel recursively iterated: as the fractal order increases, the channel path length grows exponentially, enabling outstanding sound-insulation performance within a deep-subwavelength thickness. Finite-element and transfer-matrix analyses show that increasing the fractal order from one to three raises the number of bandgaps from three to five and expands total stop-band coverage from 17% to over 40% within a deep-subwavelength thickness. Four-microphone impedance-tube measurements on the third-order sample validate a peak transmission loss of 75 dB at 495 Hz, in excellent agreement with simulations. Compared to conventional zigzag and Hilbert-maze designs, this curve fractal architecture delivers enhanced low-frequency broadband insulation, structural lightweighting, and ease of fabrication, making it a promising solution for noise control in machine rooms, ducting systems, and traffic environments. The method proposed in this paper can be applied to noise reduction of transmission parts for ceramic automation production. Full article

One of the key elements in the analysis of gene expression and its post-translational regulation is miRNAs. Degradome-seq analyses are performed to analyze the cleavage of target RNAs in the transcriptome. This work presents the first degradome-seq library preparation protocol that enables successful construction of libraries, even from highly degraded RNA samples with RIN below 3, thus significantly expanding the possibilities for research when working with low-quality material. The developed protocol improves the efficiency of library preparation in degradome-seq analysis used to identify miRNA targets, reduces library preparation time, and lowers the cost of purchasing reagents by using reagents from the RNA-seq library preparation kit and proprietary-designed primers. A crucial feature of this new protocol is optimizing the purification step for short library fragments, which increases the yield of correctly sized fragments compared to previously used methods. This is achieved by implementing an original method involving tube-spin purification with gauze and precipitation using sodium acetate with glycogen, greatly enhancing recovery efficiency—a factor especially critical when working with degraded RNA. Cloning to a plasmid and sequencing of the inserted fragment verified the correctness of the library preparation using the developed protocol. This protocol represents a groundbreaking tool for degradome research, enabling the construction and sequencing of degradome libraries, even from degraded samples previously considered unsuitable for such analyses. This is due to the use of residues from the sRNA-seq library kit. It noticeably reduces the cost of library construction. The precision of the excised fragment after electrophoresis was performed during the procedure to isolate fragments of the correct length, which was improved using additional size markers. Compared to previously used methods, optimizing the purification method of degradome-seq libraries allowed an increase in the yield of fragments obtained. Full article

The innovation in thermal storage systems for solar thermal power generation is crucial for achieving efficient utilization of new energy sources. Molten salt has been extensively studied as a phase change material (PCM) for latent heat thermal energy storage systems. In this study, a two-dimensional model of a vertical shell-and-tube heat exchanger is developed, utilizing water-steam as the heat transfer fluid (HTF) and phase change material for heat transfer analysis. Through numerical simulations, we explore the interplay between PCM solidification and HTF boiling. The transient results show that tube length affects water boiling duration and PCM solidification thickness. Higher heat transfer fluid flow rates lower solidified PCM temperatures, while lower heat transfer fluid inlet temperatures delay boiling and shorten durations, forming thicker PCM solidification layers. Adding fins to the tube wall boosts heat transfer efficiency by increasing contact area with the phase change material. This extension of boiling time facilitates greater PCM solidification, although it may not always optimize the alignment of bundles within the thermal energy storage system. Full article

The utilization of thermal energy from co-produced water during natural gas production offers a promising pathway to enhance energy efficiency and reduce carbon emissions. This study proposes a techno-economic evaluation model to assess the feasibility and profitability of geothermal energy recovery from co-produced water in marginal gas wells. A wellbore fluid flow and heat transfer model is developed and validated against field data, with deviations in calculated wellhead temperature and pressure within 10%, demonstrating the model’s reliability. Sensitivity analyses are conducted to investigate the influence of key technical and economic parameters on project performance. The results show that electricity price, heat price, and especially government one-off subsidies have a significant impact on the net present value (NPV), whereas the effects of insulation length and annular fluid thermal conductivity are comparatively limited. Under optimal conditions—including 2048 m of insulated tubing, annular protection fluid with a thermal conductivity of 0.4 W/(m·°C), a 30% increase in heat and electricity prices, and a 30% government capital subsidy—the project breaks even in the 14th year, with the 50-year NPV reaching 0.896 M$. This study provides a practical framework for evaluating and optimizing geothermal energy recovery from co-produced water, offering guidance for future sustainable energy development. Full article

This study addresses the challenge of non-uniform fracture propagation in multi-cluster staged fracturing of horizontal wells by proposing a three-dimensional dynamic simulation method for temporary plugging fracturing, grounded in a fully coupled fluid–solid damage theory framework. A Tubing-CZM (cohesive zone model) coupling model was developed to enable real-time interaction computation of flow distribution and fracture propagation. Focusing on the Xinjiang X Block reservoir, this research systematically investigates the influence mechanisms of reservoir properties, engineering parameters (fracture spacing, number of perforation clusters, perforation friction), and temporary plugging parameters on fracture propagation morphology and fluid allocation. Our key findings include the following. (1) Increasing fracture spacing from 10 m to 20 m enhances intermediate fracture length by 38.2% and improves fracture width uniformity by 21.5%; (2) temporary plugging reduces the fluid intake heterogeneity coefficient by 76% and increases stimulated reservoir volume (SRV) by 32%; (3) high perforation friction (7.5 MPa) significantly optimizes fracture uniformity compared to low-friction (2.5 MPa) scenarios, balancing flow allocation ratios between edge and central fractures. The proposed dynamic flow diversion control criteria and quantified temporary plugging design standards provide critical theoretical foundations and operational guidelines for optimizing unconventional reservoir fracturing. Full article

This paper primarily investigates the response and failure behavior of 6063-T5 aluminum alloy square tubes with varying outer side lengths under symmetric curvature-controlled cyclic bending in different bending directions. The response is characterized by the moment–curvature relationship and the variation in the outer side length with respect to curvature, whereas failure is characterized by the relationship between the controlled curvature and the number of cycles required to initiate buckling. The outer side lengths studied are 20 mm, 30 mm, 40 mm, and 50 mm, and the bending directions considered are 0°, 22.5°, and 45°. The moment–curvature curves exhibited cyclic hardening, and stable loops were formed for all outer side lengths and bending directions. An increase in the outer side length resulted in a higher peak bending moment, while a greater bending direction led to a slight increase in the peak bending moment. For a fixed bending direction, the curves representing the variation of the outer side length (defined as the change in length divided by the original length) with respect to curvature displayed symmetry, serrated features, and an overall increasing trend as the number of cycles increased, irrespective of the specific outer side length. In addition, increasing either the outer side length or altering the bending direction led to a larger variation in the outer side length. As for the relationship between curvature and the number of cycles required to initiate buckling, the data for each bending direction and each of the four outer side lengths formed distinct straight lines on a double-logarithmic plot. Based on the experimental observations, empirical equations were developed to characterize these relationships. These equations were then used to predict the experimental data and showed excellent agreement with the measured results. Full article

Background: Pneumothorax (PTX) is the most common complication of CT-guided lung biopsies. New technical advances, namely the optimization of needle approach angles within an a priori defined “safe zone,” are intended to reduce this risk. This study evaluates whether PTX incidence and chest tube placement decreased significantly after these technical advances were implemented. Methods: We retrospectively analyzed 118 consecutive patients who had undergone CT-guided lung biopsy between 9 January 2020, and 4 April 2025. The study was divided into three periods of increasingly growing institutional procedural experience: Pre-Knowledge (January 2020–March 2022; n = 45), Partial Knowledge (April–December 2022; n = 18), and Full Knowledge (January 2023–April 2025; n = 55). PTX incidence and chest tube use were compared across periods using chi-square and Fisher’s exact tests, while Kaplan–Meier survival analysis was used to evaluate PTX-free survival over time. Results: Overall PTX incidence significantly declined from 71.1% in the Pre-Knowledge Period to 21.8% in the Full Knowledge Period (p < 0.000001). Rates of chest tube placements also decreased from 17.8% to 9.1%, although this difference was not statistically significant (p = 0.372). Kaplan–Meier analysis showed a statistically significant improvement in PTX-free survival over time (indicating improvement in the timing of complication onset; p = 0.0042). Procedural optimization was also fostered by a large median intrapulmonary needle length and consistent needle angulation within the safe zone. Conclusions: Formal implementation of needle angle optimization and procedural protocol standardization has effectively reduced the frequency and severity of PTX following CT-guided lung biopsies. These results highlight the benefit of continuous education and technique standardization in improving patient safety and clinical outcomes. Full article

The natural history of focal non-infected lesions of the abdominal aorta (fl-AA) remains unclear and largely depends on their aetiology. These lesions often involve a focal “tear” or partial disruption of the arterial wall. Penetrating aortic ulcers (PAUs) and intramural hematomas (IMHs) are examples of focal tears in the aortic wall that can either progress to dilatation (saccular aneurysm) or fail to fully propagate through the medial layers, potentially leading to aortic dissection. These conditions typically exhibit a morphology consistent with eccentric saccular aneurysms. The management of focal non-infected pathologies of the abdominal aorta remains a subject of debate. Unlike fusiform abdominal aortic aneurysms, the inconsistent definitions and limited information regarding the natural history of saccular aneurysms (sa-AAAs) have prevented the establishment of universally accepted practice guidelines for their management. As emphasized in the latest 2024 ESVS guidelines, the focal nature of these diseases makes them ideal candidates for endovascular repair (class of evidence IIa—level C). Moreover, the Society for Vascular Surgery just referred to aneurysm diameter as an indication for treatment suggesting using a smaller diameter compared to fusiform aneurysms. Consequently, the management of saccular aneurysms is likely heterogeneous amongst different centres and different operators. Endovascular repair using tube stent grafts offers benefits like reduced recovery times but carries risks of migration and endoleak due to graft rigidity. These complications can influence long-term success. In this context, the use of endovascular bifurcated grafts may provide a more effective solution for treating these focal aortic pathologies. It is essential to achieve optimal sealing regions through anatomical studies of aortic morphology. Additionally, understanding the anatomical characteristics of focal lesions in challenging necks or para-visceral locations is indeed crucial in device choice. Off-the-shelf devices are favoured for their time and cost efficiency, but new endovascular technologies like fenestrated endovascular aneurysm repair (FEVAR) and custom-made devices enhance treatment success and patient safety. These innovations provide stent grafts in various lengths and diameters, accommodating different aortic anatomies and reducing the risk of type III endoleaks. Although complicated PAUs and focal saccular aneurysms rarely arise in the para-visceral aorta, the consequences of rupture in this segment might be extremely severe. Experience borrowed from complex abdominal and thoracoabdominal aneurysm repair demonstrates that fenestrated and branched devices can be deployed safely when anatomical criteria are respected. Elective patients derive the greatest benefit from a fenestrated graft, while urgent cases can be treated confidently with off-the-shelf multibranch systems, reserving other types of repairs for emergent or bail-out cases. While early outcomes of these interventions are promising, it is crucial to acknowledge that limited aortic coverage can still impede effective symptom relief and lead to complications such as aneurysm expansion or rupture. Therefore, further long-term studies are essential to consolidate the technical results and evaluate the durability of various graft options. Full article

Background and Clinical Significance: Prader–Willi syndrome (PWS) is a rare genetic disease caused by imprinted gene dysfunction, typically involving deletion of the chromosome 15q11.2-q13 region, balanced translocation, or related gene mutations in this region. PWS presents with complex and varied clinical manifestations. Abnormalities can be observed from the fetal stage and change with age, resulting in growth, developmental, and metabolic issues throughout different life stages. Case Presentation: We report the prenatal characteristics observed from the second to third trimester of pregnancy in a neonate with PWS. Prenatal ultrasound findings included a single umbilical artery, poor abdominal circumference growth from 26 weeks, normal head circumference and femur length growth, increased amniotic fluid volume after 30 weeks, undescended fetal testicles in the third trimester, small kidneys, and reduced fetal movement. The male infant was born at 38 weeks of gestation with a birth weight of 2580 g. He had a weak cry; severe hypotonia; small eyelid clefts; bilateral cryptorchidism; low responsiveness to medical procedures such as blood drawing; and poor sucking, necessitating tube feeding. Blood methylation-specific multiple ligation-dependent probe amplification (MS-MLPA) showed paternal deletion PWS. Notably, this case revealed two previously unreported prenatal features in PWS: a single umbilical artery and small kidneys. Conclusions: Through literature review and our case presentation, we suggest that a combination of specific sonographic features, including these newly identified markers, may aid clinicians in the early diagnosis of PWS. Full article

As solid-state devices continue to advance, vacuum electron devices maintain critical importance due to their superior high-frequency power handling, long-term reliability, and operational efficiency. Among these, traveling-wave tubes (TWTs) excel in high-power microwave (HPM) applications, offering exceptional bandwidth and gain. However, developing THz-range TWT slow-wave structures (SWSs) presents significant design challenges. This work systematically outlines the SWS design methodology while addressing key obstacles and their solutions. As a demonstration, a staggered double vane (SDV) SWS operating at 1 THz (980–1080 GHz) achieves 650 mW output power, 23.35 dB gain, 0.14% electronic efficiency, and compact 21 mm length. Comparative analysis with deformed quasi-sine waveguide (D-QSWG) SWS confirms the SDV design’s superior performance for THz applications. Full article

In this study, we investigated how to design a tube furnace for the oxygen annealing of a REBa₂Cu₃O_7−x (REBCO, where RE = rare earth) superconducting joint. We confirmed the annealing temperature threshold of REBCO tape I_c degradation, which was 175C. A heat exchange model that included REBCO tape and a tube furnace was established by using this temperature as the boundary condition. At the same time, the temperature distribution of the REBCO tape in a commercial tube furnace was measured for the calibration of the heat exchange model. The feasibility and accuracy of the model were confirmed by comparing the real measurements and the simulation results. We then optimized the furnace design based on the model according to two criteria: a 20 mm length of REBCO tape should be kept at high temperatures for the oxygen annealing of REBCO joints and the length of tape at temperatures over the I_c degradation temperature should be as short as possible. The results of this furnace design investigation could help fabricate shorter REBCO superconducting joints, making the magnet more compact and decreasing the length of the Cu stabilizer layer to be removed. Full article

This study innovatively proposes a pipeline-type pneumatic lift sediment removal device for cleaning pollutants at the bottom of fish breeding tanks and conducts hydrodynamic characteristic analysis on its core component, the pneumatic lift pipeline structure, which consists of a horizontal circular tube with multiple micro-orifices at the bottom and an upward-inclined circular tube. The pipeline has an inner diameter of 20 mm and a vertical length of 1.2 m, with the orifice at one end of the horizontal tube connected to the gas supply line. During operation, compressed gas enters the horizontal tube, generating negative liquid pressure that draws solid–liquid mixtures from the tank bottom into the pipeline, while buoyant forces propel the gas–liquid–solid mixture upward for discharge through the outlet. Under a constant gas flow rate, numerical simulations investigated efficiency variations through three operational scenarios: ① different pipeline orifice diameters, ② varying orifice quantities and spacings, and ③ adjustable pipeline bottom clearance heights. The results indicate that in scenario ①, an orifice diameter of 4 mm demonstrated optimal efficiency; in scenario ②, the eight-orifice configuration achieved peak efficiency; and scenario ③ showed that the proper adjustment of the bottom clearance height enhances pneumatic efficiency, with maximum efficiency observed at a clearance of 10 mm between sediment suction pipe and tank bottom. Full article