Search Results (17)

The El Tor biotype of Vibrio cholerae caused the seventh cholera pandemic (7CP). Although V. cholerae variants of this biotype frequently emerge, studies on their microevolution and spatiotemporal transmission in epidemics caused by a single clone are limited. During the cholera outbreak in Sichuan Province, China, in the 1990s, strains belonging to phage type 6 (PT6) but resistant to typing phage VP5 due to a deletion mutation in ompW, which is the gene associated with the VP5 receptor were identified. In this study, we analyzed PT6 strains using genome sequencing to reveal the genomic and transmission characteristics of such a transient phage type in China’s cholera epidemic history. The findings revealed that the PT6 strains formed an independent clone during the four-year epidemic and emerged in wave 2. Most of them carried multiple CTX^classΦ genome copies on chromosome 2 (Chr. 2) and two copies each of RS1^ET and RS1-4** on chromosome 1 (Chr. 1). Frequent cross-regional transmission and local outbreaks within Sichuan Province, China, were revealed for this clone. A variety of spontaneous mutations in the ompW gene, conferring resistance to the VP5 phage, were observed under VP5 infection pressure, showing the incident mutation of OmpW for the survival adaptation of V. cholerae to phage pressure. Therefore, this genomic epidemiological revisit of these distinct phage-resistant phenotype strains reveals their clonal genetic structure, improves our understanding of the spread of V. cholerae by tracking their variation, and assists in epidemic source tracing and disease control. Full article

Early life stress (ELS) is associated with an increased risk for neuropsychiatric disorders, and both neuroinflammation and mitochondrial dysfunction seem to be central to mental health. Herein, using an animal model of ELS, a single episode of maternal deprivation (MD, 24 h on pnd 9) extensively documented to elicit behavioural anomalies in male and female Wistar rats, we investigated its consequences in terms of neuroinflammation and mitochondrial dynamics in the prefrontal cortex (PFC) and the hippocampal formation (HCF). MD differentially affected the brain content of cytokines: MD induced a transient increase in pro-inflammatory cytokines (IL-1β and IL-6) in the PFC, as well as in the levels of the anti-inflammatory cytokine IL-10 in the HCF. MD also induced a significant decrease mitochondria citrate synthase activity, but MD did not exert significant changes in mitochondria Complex IV activity, revealing a generalized decrease in mitochondrial density without any change in mitochondrial respiration. In the present study, we demonstrate that MD induces neuroinflammatory processes in specific brain regions. Additional research is needed to better understand the temporal pattern of such changes, their impact on the developing brain, and their participation in the already well-known behavioural consequences of MD. Full article

The Grombalia aquifer constitutes a complex aquifer system formed by shallow, unconfined, semi-deep, and deep aquifers at different exploitation levels. In this study, we focused on the upper aquifer, the Wadi El Bey coastal aquifer. To assess natural aquifer recharge, we used a novel physiography-based method that uses soil texture-dependent potential infiltration coefficients and monthly rainfall data. The developed transient flow model was then applied to compute the temporal variation in the groundwater level in 34 observation wells from 1973 to 2020, taking into account the time series of spatially variable groundwater recharge, artificial groundwater recharge from 5 surface infiltration basins, pumping rates on 740 wells, and internal prescribed head cells to mimic water exchange between the wadis and aquifer. The quantified deviations in the computed hydraulic heads from measured water levels are acceptable because the database used to construct a scientifically sound and reliable groundwater model was limited. Further work is required to collect field data to quantitatively assess the local inflow and outflow rates between surface water and groundwater. The simulation of 12 climate scenarios highlighted a bi-structured north—south behaviour in the hydraulic heads: an increase in the north and a depletion in the south. A further increase in the pumping rate would, thus, be severe for the southern part of the Wadi El Bey aquifer. Full article

Southwestern Peru has an arid climate typical of subtropical west coasts bordering cold ocean currents. Mountain runoff is barely able to sustain urban needs and motivates this research. Using high-resolution satellite reanalysis products, the meso-scale climate and weather variability are explored via point-to-field regression. A time series spanning 1970–2022 of Tacna area (18 S, 70.2 W, 570 m) rainfall, potential evaporation, wind, and weather parameters were evaluated for thermodynamic and kinematic features. Although sea breezes draw marine air inland, they simultaneously generate low-level divergence and subsidence aloft. Potential evaporation in early summer causes water deficits that are rarely offset by late summer runoff from the Andes Mountains. Winter (May–September) showers from passing cold fronts are more frequent during El Niño. Warming of the tropical east Pacific accelerates subtropical westerly winds that lift over the coastal plains. Quasi-stationary Rossby wave patterns amplify transient troughs at 70 W, but the winter showers rarely exceed 4 mm/day due to low-level stability from negative heat fluxes over cool seawater offshore. Two winter wet spells were studied using satellite and surface data (July 2002, July 2009). Light showers were prominent in elevations from 400 to 900 m. An early summer dry spell was considered (November 2020), wherein southeast winds, coastal upwelling, and low dewpoint temperatures coincided with La Niña conditions. A rain-gauge transect showed that summer convection stays east of the Andes escarpment and seldom benefits the coastal plains. Thus, water resources in Tacna are strained beyond the carrying capacity. Full article

Gabal El-Ineigi fluorite-bearing rare-metal granite with A-type affinity, located in the Central Eastern Desert of Egypt, is distinguished by its abundance of large fluorite-quartz veins and mafic enclaves. Plagioclase (labradorite to oligoclase), Mg-rich biotite, and Mg-rich hornblende are the main components of mafic enclaves, with significant amounts of fluorite as essential phases, and titanite and Fe-Ti oxides (Nb-free rutile and ilmenite-rutile solid solution) as the main accessories. These enclaves are monzodioritic in composition, Si-poor, and highly enriched in Ca, Fe, Mg, and F compared to the host alkali feldspar F-poor Si-rich granites. Given the conflicting evidence for a restitic, xenolithic, magma mixing/mingling, cumulate, or bimodal origin for these enclaves, we propose that the mafic enclaves and felsic host granites are two conjugate liquids, with contrasting compositions, of a single parental melt. This is inferred by the normalized REE patterns that are similar. As a result, liquid immiscibility is proposed as a probable explanation for this mafic–felsic rock association. These enclaves can be interpreted as transient melt phases between pure silicate and calcium-fluoride melts that are preserved from the early stages of separation before evolving into a pure fluoride (Ca-F) melt during magma evolution. Due to element partitioning related to melt unmixing, the enclaves are preferentially enriched in Ca, F, Li, Y, and REE and depleted in HFSE (such as Zr, U, Th, Ta, Nb, Hf, and Ga) in comparison to the host granites. Furthermore, mafic enclaves exhibit W-type tetrad effects, while host granites exhibit M-type tetrad effects, implying that the REE partitioning, caused by liquid immiscibility, is complementary. Full article

In photovoltaic (PV) power generation, highly efficient III-V solar cells are promising for emerging mobile applications, such as vehicle-integrated PVs. Although hydride vapor phase epitaxy (HVPE) has received attention due to its lower fabrication costs, realization of high throughput performance while maintaining solar-cell characteristics using this growth method is essential. In this study, the effect of atmospheric-pressure triple-chamber HVPE growth conditions on GaAs solar-cell properties were carefully investigated in conjunction with defect analysis using deep-level transient spectroscopy (DLTS). Based on the analysis on GaAs reaction processes, the suppression of arsine thermal cracking in the HVPE hot-wall reactor was important to achieve fast GaAs growth using a low input V/III ratio. Moreover, the DLTS results revealed that the reduced input V/III ratio was effective in suppressing the generation of EL2 traps, which is a common GaAs midgap complex defect involving arsenic antisites. Although the EL2 trap density increased with the growth rate, the performance of GaAs solar cells that were grown under reduced arsine thermal cracking exhibited almost no considerable cell parameter deterioration at a growth rate of up to 297 μm/h. Consequently, a conversion efficiency of 24% with a high open-circuit voltage of 1.04 V was achieved for the cells that were grown at 200 μm/h. Full article

Time-resolved femtosecond-stimulated Raman spectroscopy (FSRS) provides valuable information on the structural dynamics of biomolecules. However, FSRS has been applied mainly up to the nanoseconds regime and above 700 cm⁻¹, which covers only part of the spectrum of biologically relevant time scales and Raman shifts. Here we report on a broadband (~200–2200 cm⁻¹) dual transient visible absorption (visTA)/FSRS set-up that can accommodate time delays from a few femtoseconds to several hundreds of microseconds after illumination with an actinic pump. The extended time scale and wavenumber range allowed us to monitor the complete excited-state dynamics of the biological chromophore flavin mononucleotide (FMN), both free in solution and embedded in two variants of the bacterial light-oxygen-voltage (LOV) photoreceptor EL222. The observed lifetimes and intermediate states (singlet, triplet, and adduct) are in agreement with previous time-resolved infrared spectroscopy experiments. Importantly, we found evidence for additional dynamical events, particularly upon analysis of the low-frequency Raman region below 1000 cm⁻¹. We show that fs-to-sub-ms visTA/FSRS with a broad wavenumber range is a useful tool to characterize short-lived conformationally excited states in flavoproteins and potentially other light-responsive proteins. Full article

The present study investigates infarct-reducing effects of blocking ischemia-induced opening of connexin43 hemichannels using peptides Gap19, Gap26 or Gap27. Cardioprotection by ischemic preconditioning (IPC) and Gap peptides was compared, and combined treatment was tested in isolated, perfused male rat hearts using function and infarct size after global ischemia, high-resolution respirometry of isolated mitochondrial and peptide binding kinetics as endpoints. The Gap peptides reduced infarct size significantly when given prior to ischemia plus at reperfusion (Gap19 76.2 ± 2.7, Gap26 72.9 ± 5.8 and Gap27 71.9 ± 5.8% of untreated control infarcts, mean ± SEM). Cardioprotection was lost when Gap26, but not Gap27 or Gap19, was combined with triggering IPC (IPC 73.4 ± 5.5, Gap19-IPC 60.9 ± 5.1, Gap26-IPC 109.6 ± 7.8, Gap27-IPC 56.3 ± 8.0% of untreated control infarct). Binding stability of peptide Gap26 to its specific extracellular loop sequence (EL2) of connexin43 was stronger than Gap27 to its corresponding loop EL1 (dissociation rate constant K_d 0.061 ± 0.004 vs. 0.0043 ± 0.0001 s⁻¹, mean ± SD). Mitochondria from IPC hearts showed slightly but significantly reduced respiratory control ratio (RCR). In vitro addition of Gap peptides did not significantly alter respiration. If transient hemichannel activity is part of the IPC triggering event, inhibition of IPC triggering stimuli might limit the use of cardioprotective Gap peptides. Full article

In central Tunisia, anthropic activities, such as groundwater abstraction for irrigation, have resulted in excessive groundwater level declines of the Mio-Plio-Quaternary aquifer hosted in Kairouan Plain. Besides, the two dams El Houareb and Sidi Saad’s impoundment since the 1980s has deeply modified the natural process of aquifer recharge. Hence, some studies claim the dam’s instauration of this groundwater depletion; however, some other studies attribute this critical situation to an issue of groundwater management. A multidisciplinary study was carried out to retrace the groundwater flow dynamics for 48 years before and after the dams’ erection and to understand the main factors causing the groundwater depletion. Hence, a conceptual model was developed based on gathering all available data from 114 borehole logs, 10 seismic lines, and 08 petroleum wells. Based on this reconstructed geometry, the groundwater head was simulated using the numerical code Modflow. The model was calibrated in steady-state with reference to the piezometric levels measured in 1969 and in the transient state for the period 1970–2017 and validated for the period 2007–2017. The outputs of the calibrated model show a relevant finding of the decrease of the inflows coming from the rivers’ beds (Zeroud and Marguellil) from 1990 to 2017 by 48%; yet, the pumping rate has increased by 119%. The simulated scenario without dams and maintaining the same withdrawals has shown a groundwater level rise downstream of the plain; yet, in its upstream, the depletion was less intense compared to the current model. However, the case of doing without dams and raising withdrawals from the aquifer has generated a huge decline reaching 22 m near Draa Affane. Full article

Non-synchronous blade vibrations have been observed in an experimental multi-stage high-speed compressor setup at part-speed conditions. A detailed numerical study has been carried out to understand the observed phenomenon by performing unsteady full-annulus Reynolds-Averaged Navier–Stokes (RANS) simulations of the whole setup using the solver elsA. Several operating conditions have been simulated to observe this kind of phenomena along a speedline of interest. Based on the simulation results, the physical source of the non-synchronous blade vibration is identified: An aerodynamic disturbance appears in a highly loaded downstream rotor and excites a spinning acoustic mode. A “lock-in” phenomenon occurs between the blade boundary layer oscillations and the spinning acoustic mode. The establishment of axially propagating acoustic waves can lead to a complex coupling mechanism and this phenomenon is highly relevant in understanding the multi-physical interactions appearing in modern compressors. It is shown that aerodynamic disturbances occurring downstream can lead to critical excitation of rotor blades in upstream stages due to an axially propagating acoustic wave. The paper includes the analysis of a relevant transient test and a detailed analysis of the numerical results. The study shows the capability and necessity of a full-annulus multistage simulation to understand the phenomenon. Full article

Constructive interference of lightning-generated signals in the extremely low frequency (ELF) below 100 Hz is the source of a global electromagnetic phenomenon in the Earth’s atmosphere known as Schumann Resonances (SR). SR are excited at frequencies of 7.8, 14, 20, 26, … Hz, and their diurnal and seasonal intensity variations are largely dependent on changes in the location and magnitude of the major lightning centres in Southeast Asia, Africa, and South America. In the last five decades, extensive research has focused on reconstructing the spatial and temporal evolution in global lighting activity using SR measurements, and more recently on analysing the links to climate change, transient luminous events (TLE), and biological systems. In this study, a quasi-electrostatic antenna, primarily designed as a thunderstorm warning system, is for the first time applied to measure background variability in the SR band at an urban site in Southwest England. Data collected continuously from June 2015 for a 5-year period are suitably filtered and analysed showing that SR is the dominant contribution to the fair-weather displacement current measured by the sensor in the band 10–45 Hz. Diurnal and seasonal signal amplitude variations have been found to be consistent with previous studies and show the African-European lightning centre to prevail due to the shorter source-observer distance. Also, it is shown that long-term global changes in the ocean and land temperature, and the subsequent effect on the major lightning hotspots, may be responsible for the inter-annual variability of SR intensity, indicating that the largest increase occurred during the 2015–2016 super El-Niño episode. Full article

The storage of charges in organic light-emitting diodes (OLEDs) has drawn much attention for its damage to device performance as well as the loss to carriers. Thus, it is essential to address the issue and do further investigation. The traditional approach to storage analysis is mainly based on transient measurement since it is sensitive to transient instead of steady signal. In this paper, we proposed a new benchmark to investigate the single-layer OLEDs capable of stored charges with poly (methyl methacrylate) (PMMA), which is just based on electrical and optical characteristics. Since the stored charges contribute both to luminance and current of the devices with PMMA, the area between them can be taken as a benchmark and evaluated the storage of charges. In our experiment, the areas of 4 nm, 6 nm, 8 nm, and 10 nm PMMA devices are 0.348, 0.554, 0.808, and 0.894, respectively, indicating a higher capability of storage in thicker PMMA. It is exactly in line with the results taken from transient electroluminescence (EL) measurement. Thus, this new benchmark is practical and provides a more accessible approach to investigate the storage of charges in OLEDs. Full article

To face the intensive use of natural gas and other fossil fuels to generate hydrogen, water electrolysis based on renewable energy sources (RES) seems to be a viable solution. Due to their fast response times, and high efficiency, proton exchange membrane electrolyzer (PEM EL) is the most suitable technology for long-term energy storage, combined with RES. Like fuel cells, the development of fit DC-DC converters is mandatory to interface the EL to the DC grid. Given that PEM EL operating voltages are quite low and to meet requirements in terms of output current ripples, new emerging interleaved DC-DC converter topologies seem to be the best candidates. In this work, a three-level interleaved DC-DC buck converter has been chosen to supply a PEM EL from a DC grid. Therefore, the main objective of this paper is to develop a suitable control strategy of this interleaved topology connected to a PEM EL emulator. To design the control strategy, investigations have been carried out on energy efficiency, hydrogen flow rate, and specific energy consumption. The obtained experimental results validate the performance of the converter in protecting the PEM EL during transient operations while guaranteeing correct specific energy consumption. Full article

Nicotinic acid adenine dinucleotide phosphate (NAADP) gates two-pore channels 1 and 2 (TPC1 and TPC2) to elicit endo-lysosomal (EL) Ca²⁺ release. NAADP-induced EL Ca²⁺ signals may be amplified by the endoplasmic reticulum (ER) through the Ca²⁺-induced Ca²⁺ release mechanism (CICR). Herein, we aimed at assessing for the first time the role of EL Ca²⁺ signaling in primary cultures of human metastatic colorectal carcinoma (mCRC) by exploiting Ca²⁺ imaging and molecular biology techniques. The lysosomotropic agent, Gly-Phe β-naphthylamide (GPN), and nigericin, which dissipates the ΔpH which drives Ca²⁺ refilling of acidic organelles, caused massive Ca²⁺ release in the presence of a functional inositol-1,4,5-trisphosphate (InsP₃)-sensitive ER Ca²⁺ store. Liposomal delivery of NAADP induced a transient Ca²⁺ release that was reduced by GPN and NED-19, a selective TPC antagonist. Pharmacological and genetic manipulations revealed that the Ca²⁺ response to NAADP was triggered by TPC1, the most expressed TPC isoform in mCRC cells, and required ER-embedded InsP₃ receptors. Finally, NED-19 and genetic silencing of TPC1 reduced fetal calf serum-induced Ca²⁺ signals, proliferation, and extracellular signal-regulated kinase and Akt phoshorylation in mCRC cells. These data demonstrate that NAADP-gated TPC1 could be regarded as a novel target for alternative therapies to treat mCRC. Full article

The acidic vesicles of the endolysosomal (EL) system are emerging as an intracellular Ca²⁺ store implicated in the regulation of multiple cellular functions. The EL Ca²⁺ store releases Ca²⁺ through a variety of Ca²⁺-permeable channels, including Transient Receptor Potential (TRP) Mucolipin 1-3 (TRPML1-3) and two-pore channels 1-2 (TPC1-2), whereas EL Ca²⁺ refilling is sustained by the proton gradient across the EL membrane and/or by the endoplasmic reticulum (ER). EL Ca²⁺ signals may be either spatially restricted to control vesicle trafficking, autophagy and membrane repair or may be amplified into a global Ca²⁺ signal through the Ca²⁺-dependent recruitment of ER-embedded channels. Emerging evidence suggested that nicotinic acid adenine dinucleotide phosphate (NAADP)-gated TPCs sustain multiple cancer hallmarks, such as migration, invasiveness and angiogenesis. Herein, we first survey the EL Ca²⁺ refilling and release mechanisms and then focus on the oncogenic role of EL Ca²⁺ signaling. While the evidence in favor of TRPML1 involvement in neoplastic transformation is yet to be clearly provided, TPCs are emerging as an alternative target for anticancer therapies. Full article