Search Results (1,350)

Integrating advanced technologies, such as Connected Autonomous Vehicles (CAVs) and Connected Vehicles (CVs), represents new strategies and solutions in urban mobility, particularly during unexpected urban events. Vehicle connectivity facilitates real-time communication between vehicles and infrastructure, enhancing traffic management by enabling dynamic rerouting to minimize delays and prevent bottlenecks. This study employs the SUMO (Simulation of Urban Mobility) microsimulation to analyze the impact of dynamic rerouting strategies during urban disruptions within the IN2CCAM project’s Turin Living Lab. The Living Lab integrates simulation with real-world testing, including autonomous shuttle operations, to evaluate new mobility solutions. In the initial phase, offline simulations examine street, lane, and intersection closures along shuttle routes to assess how penetration levels of CVs and CAVs influence mobility. The results indicate that higher connectivity penetration improves traffic flow, with the greatest benefits observed at increased levels of autonomous vehicles. These findings highlight the potential of dynamic routing strategies, supported by vehicle connectivity and autonomous driving technologies, to enhance urban mobility and effectively respond to real-time traffic conditions. Additionally, this work demonstrates the capabilities and flexibility of SUMO for simulating complex urban traffic scenarios. Full article

Background/Objectives: Treatment of hard-to-heal wounds remains a challenge in clinical practice. This study aimed to assess the real-world efficacy of a novel porcine placental extracellular matrix (PPECM; InnovaMatrix^® AC, Convatec, Memphis, TN, USA). Methods: This single-center, retrospective study analyzed consecutive electronic medical records of patients receiving wound care between May 2022 and May 2024. Eligible patients had a hard-to-heal wound that failed to achieve sufficient healing after a minimum of 4 weeks of standard of care treatment, and went on to subsequent management with PPECM. Results: Eighty-nine patients (median age, 79 years) were included. The most common etiologies were traumatic wounds (30.3%), venous ulcers (25.8%), surgical wounds (21.3%), and diabetic ulcers (10.1%). Median wound age at first PPECM application was 11.4 weeks (range, 4.0–484.9). The median percent area reduction of the target wound in weeks 4, 12, and 20 was 43.33% (95% CI, 32.92–64.29; n = 75), 100.00% (95% CI, 100.00–100.00; n = 65), and 100.00% (95% CI, 100.00–100.00; n = 58), respectively. Wound closure occurred in 56 (62.9%) patients. The Kaplan–Meier estimate for the median time to complete wound closure was 66 days (95% CI, 55–96; n = 56) and that for the probability of wound closure in weeks 4, 12, and 20 was 0.21 (95% CI, 0.05–0.35), 0.62 (95% CI, 0.40–0.76), and 0.83 (95% CI, 0.31–0.96), respectively. Conclusions: Clinically stalled hard-to-heal wounds displayed a clinically relevant reduction in wound area at 4 weeks following treatment with PPECM, with a high probability of wound closure at 12 and 20 weeks after starting treatment. PPECM may support the closure of hard-to-heal wounds that have failed to respond to standard of care treatment. Full article

Hyperglycemia, which arises in type 1 or 2 diabetes, leads to different complications, such as macrovascular disease, nephropathy, retinopathy, and neuropathy. In addition, different cognitive variations are associated with type 1 diabetes. Long-term changes in glucose metabolism might induce effects on the central nervous system (CNS) such as reduced mental performance and loss of consciousness, which could be implicated in neurotoxicity. The direct impact of hyperglycemia and elevated glucose concentrations on neuronal cells remains to be fully elucidated, primarily due to the multifaceted mechanisms underlying glucose neurotoxicity, including apoptosis, oxidative stress, and alterations in signaling cascades. The multifaceted mechanisms further complicate the study of the relationship between diabetes and neurodegeneration. Research in this field is continually advancing, with the aim of investigating these eventual connections and developing more effective preventive and therapeutic strategies. The present study aims to assess the damage induced by different glucose concentrations (from 25 to 150 mM) in a neuronal model, such as PC12 cells, rat pheochromocytoma cells. In glucose-exposed PC12 cells, we have tested oxidative stress, apoptosis, and cell migration by (a) viability screening, (b) intracellular levels of anion superoxide (O₂⁻), (c) extracellular levels of MDA and nitrites, (d) apoptosis, and (e) the wound healing assay. By the cell viability assay, it has emerged that glucose (25–150 mM) showed a stronger effect at the highest concentrations (100 and 150 mM). The increase in MDA and O₂⁻ levels was determined in PC12 cells treated with high glucose concentrations (6.5–8.8 fold for MDA). High concentrations (100 and 150 mM) significantly reduced the expression of full-length caspase-3 (2.8-fold and 4.2-fold decrease at 24 and 72 h) and caspase-9 (3.4-fold and 2.8-fold decrease at 24 h and 5-fold decrease at 72 h) compared with control conditions. Finally, the wound healing assay showed different scenarios during the several time points. Indeed, the wound closure rate was reduced in a dose-dependent manner (24 h: control 18%, G 50 mM 9%, 100 and 150 mM 8%; 48 h: control 26%, G 50 mM 20%, G 100 mM 13%, 150 mM 11%), following the treatment with three concentrations considered (50, 100, 150 mM). The results obtained in these experimental conditions highlight that glucose, at high concentrations, induced cell damage and corroborate the hypothesis that it could be involved in neurodegenerative diseases. Full article

Acute appendicitis remains one of the most common surgical emergencies, with a lifetime incidence of approximately 7–8% in the USA and Europe. Despite the widespread adoption of the laparoscopic approach and advances made in perioperative care, post-operative infections—particularly intra-abdominal abscesses—continue to pose a substantial clinical challenge, with an overall probability that ranges from 5 to 15%. Nowadays, it is essential not only to improve patient outcomes by reducing these complications but also to promote responsible antibiotic use. This review provides an in-depth examination of post-appendectomy infections in adults, synthesizing research from the past decade. It explores the various risks involved, including those related to the patient, the disease itself, and the surgical techniques employed. There is particular emphasis on the impact of surgical approach, closure methods, timing of surgery, and intraoperative decisions such as drain placement, peritoneal lavage, and routine bacterial cultures. Part of the discussion is about emerging data regarding the use of antiseptic solutions and specimen retrieval techniques. Additionally, the review examines current approaches to managing postoperative intra-abdominal abscesses. It assesses when antibiotics are necessary, evaluates image-guided percutaneous drainage, and considers laparoscopic re-intervention as a possible solution. While recent studies offer valuable insights, the heterogeneity of available evidence highlights the pressing need for high-quality, standardized research. Ultimately, a deeper understanding of infection pathways and preventative strategies is vital—not only for reducing morbidity and hospital readmissions, but also for safeguarding the long-term efficacy of antibiotics and delivering safer, more effective surgical care. Full article

Background and Clinical Significance: Atrial septal defects (ASDs), particularly the ostium secundum type, are congenital cardiac anomalies that can lead to serious complications if left untreated. Percutaneous closure using devices like the Amplatzer Septal Occluder (ASO) has become a widely accepted approach, although complications such as device embolization can occur. Case Presentation: We present a unique case of a 28-year-old woman who developed acute hemodynamic instability and arrhythmias following embolization of an Amplatzer device into the right ventricle during an ASD closure. Despite initial treatment with antiarrhythmic medication, the patient required urgent open-heart surgery for device retrieval and ASD closure. The surgery successfully involved pericardial patch closure of the ASD, device removal from the right ventricle, and the performance of the Kay procedure to address significant tricuspid regurgitation. Postoperative recovery was uneventful, with the patient stabilized and discharged in stable condition. Conclusions: This case highlights the critical need for rapid surgical intervention in cases of device embolization, and the importance of multidisciplinary coordination in managing such complex complications. The combination of ASD closure, device retrieval, and tricuspid valve repair led to a successful outcome, underscoring the importance of timely, decisive action in complex cardiovascular emergencies. Full article

Urban tree biodiversity represents a valuable natural resource. However, some fast-growing tree species with limited esthetic value play an important ecological role by colonizing degraded areas, such as closed landfills. Our observations indicate that trees like Betula pendula (Roth), Acer negundo (L.), and Populus tremula (L.) reached the size of adult trees in less than 30 years after the landfill’s closure in the 1990s, forming a nature area similar to a natural forest. A resident survey conducted among the inhabitants of Zgierz confirmed that the lack of space provides opportunities for various forms of recreation. The example analyzed indicates a trend that can be replicated in other cities with minimal human intervention and low financial costs for landfill reclamation. The case study presents an ecological approach to managing degraded sites, where nature determines the quality of the soil environment by eliminating pollutants from the residential surroundings. Furthermore, the research framework provides a basis for developing future models for cleaning up urban landfill sites and promoting placemaking. This pilot study shows a model for old landfills in Europe with well-developed spontaneous vegetation that can be transformed into recreation and sports facilities in the urban areas with industrial past times. Full article

During the sudden closure of gates in long-distance gravity flow water supply projects, intense water hammer waves are generated. These waves can cause severe damage to the water supply tunnel structure, posing a significant threat to project safety. To develop an economical and effective hydraulic safety control strategy, this study uses the example of a specific gravity flow water supply project with long-distance and multi-fluctuation pressure tunnels in Zhejiang Province. A novel combined protection strategy was investigated, involving the conversion of construction branch tunnels into branch tunnel surge tanks combined with an overflow surge tank. Numerical simulations of gate closure-induced water hammer pressures were conducted using the method of characteristics. Additionally, the effectiveness of the overflow surge tank on controlling the surge water level in the branch tunnels was analyzed with respect to variations in its height, diameter, and impedance hole diameter. The results indicate that a 300 s linear gate closure without any protective measures induces severe water hammer pressure. Extending the closure time to 1200 s still results in pressures far exceeding the safety threshold. Converting construction branch tunnels into surge tanks effectively controlled the water hammer pressure; however, overflow issues emerged in some branch tunnels. The subsequent addition of an overflow surge tank at the end of the water supply system successfully eliminated the risk of overflow in the branch tunnels. Building upon this, multi-parameter optimization analysis was used to determine the optimal configuration for the overflow surge tank. This solution ensures hydraulic safety while maintaining cost-effectiveness. Both the maximum pressure and the minimum pressure along the water supply tunnel, as well as the surge water levels in all branch tunnels, meet the code requirements. Furthermore, the reduced size of the surge tank significantly lowered construction costs. The findings of this research provide theoretical foundations and technical support for similar long-distance gravity flow water supply projects. Full article

Background and Objectives: Diabetes mellitus (DM) is associated with impaired wound healing and increased oxidative stress, posing a significant challenge in dental wound healing. Low-level laser therapy (LLLT) has emerged as a potential regenerative treatment to enhance tissue repair. This study aims to investigate the effects of LLLT on oral mucosal wound healing and oxidative stress markers in rats with DM. Materials and Methods: Male Wistar rats (n = 108) were divided into six equal groups (healthy and diabetic, with or without mucosal ulcers, with or without LLLT). DM was induced with alloxan, and standardized mucosal ulcers were created. Every other day for 10 days, LLLT (6 J/cm²) was applied, and tissue samples were collected after 3, 7, and 10 days. Wound healing was assessed by planimetry, while systemic biochemical analyses included total oxidative status (TOS), total antioxidant capacity (TAC), superoxide dismutase (SOD) activity, and oxidative stress index (OSI). Results: LLLT significantly accelerated oral ulcer closure and showed between-group differences in redox markers. In healthy rats, LLLT increased wound closure on day 7 (p = 0.018). In diabetic rats, LLLT improved closure on day 3 (p = 0.035) and on day 7 (p = 0.001). Across groups, oxidative markers differed significantly (e.g., TOS on day 10 overall, p = 0.011; OSI on day 10 overall, p = 0.047; SOD p < 0.001 at all time points). In diabetic rats, on day 10, median TOS was lower with LLLT (p = 0.004). Conclusions: LLLT enhances oral wound healing and restores redox balance in diabetic rats, which supports the potential usage of LLLT as an adjunctive therapy for managing oral lesions in diabetic patients. Full article

Background/Objectives: Periprosthetic joint infection (PJI) is a challenging problem in orthopedic surgery and is often associated with high morbidity. The treatment becomes even more challenging whenever the microorganism is virulent and/or not widely known as a causative organism on these occasions. This study aims to report on the clinical outcomes of hip hemiarthroplasty prosthetic hip joint infection by an atypical, rare microorganism, Morganella morganii (M. morganii), focusing on morbidity, revisions, and mortality. Methods: This is a retrospective series of four cases of prosthetic joint infections with Morganella morganii, a rare Gram-negative opportunistic facultative anaerobic pathogen, in four patients who received hip hemiarthroplasty for displaced femoral neck fractures at a level 1 trauma center. Clinical notes, laboratory findings, and radiographs were reviewed to extract relevant information regarding the history and outcomes. Results: The patients were four females, with a mean age of 84.27 years at the time of surgery. Two cases (50%) underwent surgical debridement and implant retention, followed by lifelong antibiotic suppression for symptomatic control of persistent wound drainage, and the other two underwent implant removal and resection arthroplasty (one patient) or received an antibiotic spacer (one patient), followed by chronic antibiotic therapy until wound closure. Conclusions: Periprosthetic hemiarthroplasty infection secondary to M. morganii was associated with overall poor outcomes. Antibiotic suppression could be a reasonable option after the surgical debridement or implant removal in M. morganii PJI to control the symptoms. Full article

Mathematical modeling is indispensable in oncology for unraveling the interplay between tumor growth, vascular remodeling, and therapeutic resistance. We present a hybrid modeling framework (continuum-discrete) and present its hybrid mathematical formulation as a coupled partial differential equation–agent-based (PDE-ABM) system. It couples reaction–diffusion fields for oxygen, drug, and tumor angiogenic factor (TAF) with discrete vessel agents and stochastic phenotype transitions in tumor cells. Stochastic phenotype switching is handled with an exact Gillespie algorithm (a Monte Carlo method that simulates random phenotype flips and their timing), while moment-closure methods (techniques that approximate higher-order statistical moments to obtain a closed, tractable PDE description) are used to derive mean-field PDE limits that connect microscale randomness to macroscopic dynamics. We provide existence/uniqueness results for the coupled PDE-ABM system, perform numerical analysis of discretization schemes, and derive analytically tractable continuum limits. By linking stochastic microdynamics and deterministic macrodynamics, this hybrid mathematical formulation—i.e., the coupled PDE-ABM system—captures bidirectional feedback between hypoxia-driven angiogenesis and resistance evolution and provides a rigorous foundation for predictive, multiscale oncology models. Full article

Growing concern over cetacean welfare has highlighted the need for rigorous, science-based assessment methods. Within this context, epidermal cortisol (ECC) and oxytocin (EOC) concentrations have emerged as potentially valuable physiological indicators. In this study, we first validated the analytical measurement of ECC and EOC in bottlenose dolphins (Tursiops truncatus) using AlphaLISA assays. Subsequently, weekly ECC and EOC levels were measured over an extended period in five managed dolphins and analyzed alongside aggregated environmental and welfare-related variables, using various time lags to account for delays between physiological activity and hormone deposition in the epidermis. ECC was negatively associated with mild weight loss and diazepam administration, exhibiting seasonal variability. In contrast, EOC was negatively associated with negative welfare indicators and COVID-19 park closures but positively associated with diazepam administration and peak visitor seasons, also showing seasonal variability. However, the interpretation of EOC remains complex due to a limited understanding of the cetacean oxytocin system and its dual role in positive and negative affective states. Overall, ECC and EOC show promise as non-invasive biomarkers for monitoring long-term welfare changes in cetaceans, although further research is necessary to validate these biomarkers across broader populations and contexts and to clarify their temporal dynamics in the epidermis. Full article

Over the past 15 years, Nodularia spumigena blooms in the Gulf of Gdańsk (southern Baltic Sea) have reached exceptional intensity, accounting for up to 90% of phytoplankton biomass during peak summer periods. Our long-term observations revealed oscillations in blooms intensity, with peak nodularin concentrations (up to 45,000 μg/L) recorded in 2012, 2015, and 2018—the highest levels of this toxin documented to date in both the Baltic Sea and worldwide. An extreme cyanobacterial bloom in 2018, caused by unusually high air and water temperatures, covered almost the whole surface of the Gulf of Gdańsk, causing multi-day closures of bathing areas. During this bloom, high levels of microcystins (up to 6640 μg/L MC-LR) were also detected, as well as the presence of 42 cyanopeptides, mainly anabaenopeptides and spumigins, which were present at concentrations 5–10 times higher than hepatotoxins. Full article

Lithium-ion batteries (LIBs) have become the dominant energy storage technology due to their versatility and superior performance across diverse applications. Silicon (Si) stands out as a particularly promising high-capacity anode material for next-generation LIBs, offering a theoretical capacity nearly ten times greater than conventional graphite anodes. However, its practical implementation faces a critical challenge: the material undergoes a ~300% volume expansion during lithiation/delithiation, which causes severe mechanical stress, electrode pulverization, and rapid capacity decay. In addressing these limitations, advanced polymer binders serve as essential components for preserving the structural integrity of Si-based anodes. Notably, self-healing polymeric binders have emerged as a groundbreaking solution, capable of autonomously repairing cycle-induced damage and significantly enhancing electrode durability. The evaluation of self-healing performance is generally based on mechanical characterization methods while morphological observations by scanning electron microscopy provide direct evidence of crack closure; for electrochemically active materials, electrochemical techniques including GCD, EIS, and CV are employed to monitor recovery of functionality. In this study, a novel self-healing copolymer (PHX-23) was synthesized for Si anodes using a combination of octadecyl acrylate (ODA), methacrylic acid (MA), 2-hydroxyethyl methacrylate (HEMA), and polyethylene glycol methyl ether methacrylate (PEGMA). The copolymer was thoroughly characterized using NMR, FTIR, TGA, SEM, and EDX to confirm its chemical structure, thermal stability, and morphology. Electrochemical evaluation revealed that the PHX-23 binder markedly improves cycling stability, sustaining a reversible capacity of 427 mAh g⁻¹ after 1000 cycles at 1C. During long-term cycling, the Coulombic efficiency of the PHX-23 polymer is 99.7%, and similar functional binders in the literature have shown similar results at lower C-rates. Comparative analysis with conventional binders (e.g., PVDF and CMC/SBR) demonstrated PHX-23’s exceptional performance, exhibiting higher capacity retention and improved rate capability. These results position PHX-23 as a transformative binder for silicon anodes in next-generation lithium-ion batteries. Full article

In cemented endoprosthetics, closed prepacked mixing systems represent the most advanced generation of cementing technology. (1) Background: The purpose of the present study is to evaluate four approved prepacked systems—Palacos^® R+G pro, SmartMix™ Cemvac GHV, Optipac^® Refobacin and Cemex^® System Genta—with a focus on practical handling and intraoperative hygiene. (2) Method: The systems were evaluated according to established standard test methods for bone cements (ISO 5833), including dough time, setting time, additional mechanical tests and the level of system closure. (3) Results: The results show that all systems are safe to use and meet the general requirements, but there are relevant differences in terms of intraoperative hygiene. The Palacos R+G pro system shows significantly shorter doughing and setting times, which helps to minimize wound exposure during surgery and thus significantly reduces the overall operating time and the risk of bacterial contamination. Two of the systems cannot be classified as completely closed “pre-packaged systems.” In two cases, the system must be temporarily opened before mixing to insert the mixing element, which may result in a temporary but clinically relevant impairment of sterility and a corresponding potential risk of contamination. (4) Conclusion: From a hygienic point of view, systems that remain completely closed throughout the entire preparation process can offer advantages in terms of infection prevention. This was the case for all systems tested. Short handling times, reduced exposure of the surgical site and a shorter overall duration of the procedure could further improve intraoperative safety and reduce the risk of contamination. In terms of intraoperative hygiene, the Palacos R+G pro system achieved the best results compared to the three other systems tested due to its rapid readiness for use and comparatively short setting time (according to ISO 5833). Cemex System Genta performed worst in this respect due to its late doughing time and setting time. Full article

Floyd–Warshall’s algorithm is a widely-known procedure for computing all-pairs shortest paths in a graph of n vertices in $\Theta \left(n^3\right)$ time complexity. A simplified version of the same algorithm computes the transitive closure of the graph with the same time complexity. The algorithm operates on an $n\times n$ matrix, performing n inspections and no more than n updates of each matrix cell, until the final matrix is computed. In this paper, we apply a technique called SmartForce, originally devised as a performance enhancement for solving the traveling salesman problem, to avoid the inspection and checking of cells that do not need to be updated, thus reducing the overall computation time when the number, u, of cell updates is substantially smaller than $n^3$. When the ratio $u/n^3$ is not small enough, the performance of the proposed procedure might be worse than that of the Floyd–Warshall algorithm. To speed up the algorithm independently of the input instance type, we introduce an effective hybrid approach. Finally, a similar procedure, which exploits suitable fast data structures, can be used to achieve a speedup over the Floyd–Warshall simplified algorithm that computes the transitive closure of a graph. Full article