Search Results (3,727)

Despite recent advances in artificial intelligence for agriculture, reliable crop recommendation remains constrained by limited access to soil diagnostics, insufficient integration of environmental context, and the absence of transparent, quantitative evaluation frameworks. This study addresses the research question: How can we integrate multiple indicators to generate accurate, explainable, and context-sensitive crop recommendations? To this end, we propose a multimodal decision-support framework that combines image-based soil texture classification with geospatial, and climatic information. A convolutional neural network was trained on a curated dataset of 3250 soil images aggregated from four publicly available sources, covering four primary soil texture classes, alongside tabular soil and nutrient data. The model was evaluated using 5-fold stratified cross-validation, achieving an average classification accuracy of 99.30% (standard deviation ≈ 0.66), and was further validated on an independent hold-out test set to assess generalization performance. To enhance practical applicability, the framework incorporates elevation, rainfall, temperature, and major soil nutrients, and employs a large language model to generate user-oriented, interpretable justifications for each recommendation. Crop recommendations were quantitatively evaluated using a novel Agronomic Suitability Score (ASS), which measures alignment across soil compatibility, climatic suitability, seasonal alignment, and elevation tolerance. Across six geographically diverse case studies, the framework achieved mean ASS values ranging from 3.76 to 4.96, with five regions exceeding 4.45, demonstrating strong agronomic validity, robustness, and scalability. A Streamlit-based application further illustrates the system’s ability to deliver accessible, location-aware, and explainable agronomic guidance. The results indicate that the proposed approach constitutes a scalable decision-support tool with significant potential for sustainable agriculture and food security initiatives. Full article

High-temperature (HT) stress during flowering causes male sterility and yield loss in soybean. MYB transcription factors are key regulators under abiotic stress, yet their function and mechanism in regulating male fertility under HT stress in soybean are not fully understood. In this study, a MYB transcription factor GmMYB21a in soybean was identified. GmMYB21a was induced by HT stress in soybean restorer line and was specifically expressed in pollen. Through overexpression and knockout experiments, we demonstrated that GmMYB21a positively regulated pollen viability and germination under HT stress. Overexpression of GmMYB21a significantly enhanced these traits in restorer line, whereas knockout plants exhibited the opposite effect. Transcriptome sequencing revealed that GmMYB21a overexpression upregulated numerous stress-responsive genes, particularly those involved in flavonoid biosynthesis and sugar metabolism. In addition, molecular experiments confirmed that GmMYB21a bound to the promoter of flavonoid synthesis gene GmCHI2-A and promoted its expression. In summary, our research indicated GmMYB21a enhanced the HT-tolerance of male fertility in soybean restorer line through reactive oxygen species scavenging and flavonoid synthesis. This study aims to elucidate the thermotolerance mechanism in soybean male fertility and identify genetic resources for breeding HT-tolerant restorer lines. Full article

For the western Patagonia region (Argentina), climate change models predict a 1–3 °C increase in temperature and a 10%–30% reduction in precipitation. Patagonia native forests are home to a variety of soil fungi, including ectomycorrhizal fungi (EcM), which play a crucial role in drought-tolerant trees. However, the responses of soil fungi to changes in rainfall remain poorly understood. To evaluate shifts in soil fungal communities’ response under reduced precipitation scenarios and identify potentially drought-tolerant EcM species, we took 144 composite soil samples associated with Nothofagus forests along an east–west rainfall gradient. We used environmental DNA to estimate alpha and beta diversity of soil fungi and EcM. Soil fungal richness did not differ across precipitation treatments, whereas EcM richness declined with decreasing precipitation. Shannon and inverse Simpson indices of EcM decreased under reduced precipitation, whereas no significant effects were observed on soil fungi, highlighting the EcM vulnerability to water limitation. Soil fungi community composition changes along the rainfall gradient due to species replacement. Tarzetta sp., Cortinarius sp., and Russula sp. were found in drier plots, indicating a potential association with drought tolerance. Selecting native drought-tolerant EcM for forest management and restoration can improve seedling establishment and ecosystem resilience under climate change. Full article

In this study, a thermally crosslinking clay-free weak gel water-based drilling fluid based on salt-responsive polymers and crosslinking agents was investigated as a promising and feasible strategy. Firstly, a salt-tolerant polymer was synthesized using N,N-dimethylacrylamide (DMAA), [2-(methacryloyloxy)ethyl]dimethyl-(3-sulfonopropyl)ammonium hydroxide (DMAPS), and acrylamide (AM). BPEI_10,000 was selected as the thermal crosslinking agent. The optimal crosslinking was achieved at 180 °C and 36% NaCl, with RMFL at 2.0% and BPEI_10,000 at 0.1%. Performance evaluation demonstrated that the crosslinking between RMFL and BPEI_10,000 could enhance the AV, PV, and YP of the RMFL(BPEI_10,000)/CF-WBDFs after aging at 180 °C for 16 h and reduce FL_API. The RMFL(BPEI_10,000)/CF-WBDFs exhibited appropriate shear-thinning behavior, viscoelasticity, thixotropy, and recoverable viscosity under high-temperature, high-salinity, and high-pressure conditions. Mechanism analysis revealed that RMFL and BPEI_10,000 could form a predominantly negatively charged, three-dimensional crosslinking weak gel at high temperatures. The crosslinking weak gel could form dense filter cakes, improving rheological properties and reducing filtration loss of CFWBDFs in high-temperature, high-salinity environments. This paper proposed a novel method to address the technical challenge of rheological performance failure of CFWBDFs, offering valuable insights for subsequent investigations. Full article

Flexible conductive hydrogels hold great promise in wearable electronics and biomonitoring applications, yet their practical use is constrained by issues such as poor low-temperature tolerance, susceptibility to dehydration, and limited multifunctional sensing capabilities. This study successfully synthesized a dual-conductive lithium-ion and calcium-ion hydrogel based on acrylamide/gelatin via a simplified low-temperature one-pot polymerization method. At 60 °C, mixing acrylamide, gelatin, lithium chloride, and calcium chloride within 40 min constructed a network structure featuring covalent bonds, ionic bonds, and hydrogen bonds. The resulting material exhibited exceptional extensibility with a break elongation of 1408.5% and tensile strength of 134.2 kPa while maintaining strong adhesion to nine different substrates. It retained flexibility at −20 °C and demonstrated minimal mass loss (3% of initial value) after 10 days of aging. As a sensor, this hydrogel reliably responds to pressure, temperature, large-amplitude body movements, and subtle physiological signals like pulse and vocal cord vibrations. In animal simulation monitoring, its electrical resistance signals increased linearly with model body weight and remained stable between −20 °C and 20 °C. These results demonstrate the hydrogel’s broad application potential in wearable sensing, ecological monitoring, and smart agriculture. Full article

Salmonella enterica remains a major food safety concern in poultry, and processing-related stress can influence its survival and persistence. This study evaluated the phenotypic expression of S. enterica serotypes Kentucky (SK), Infantis (SI), Schwarzengrund (SS), and Typhimurium (ST) following antimicrobial and temperature stressors. A pre-harvest isolate of each serotype was gradually exposed to increasing concentrations of peracetic acid (PAA) and quaternary ammonium compounds (QACs), starting at 40 ppm and 1 ppm, respectively, until minimum inhibitory and bactericidal concentrations (MICs/MBCs) were established. Stressed cells were then subjected to cold (4 °C, 60 min) and heat (55 °C, 6 min) shock and assessed for sanitizer tolerance, biofilm formation and recovery, and antibiotic resistance. Sanitizer tolerance after daily conditioning varied among S. enterica serotypes, with ST and SK showing the highest tolerance to PAA and QACs, respectively. The tolerance of PAA variants increased by 10–20 ppm and QAC variants by 2–8 ppm following stress exposure. The double-stressed variants of ST significantly (p < 0.05) formed more biofilm than the control after PAA, whereas no significant differences were observed among the variants for other serotypes. Biofilm recovery was higher for the stressed variants of SI and SS (p < 0.05) following PAA stress but remained the same across all serotypes after QAC stress. QAC-stressed variants showed more phenotypic changes across the antibiotics tested. Notably, the stressed variants of SK, SS, and ST displayed increased MICs, including a 2- to 4-fold rise in azithromycin for the SK and ST variants. There was an increase in the MICs of ceftriaxone and nalidixic acid for some SK and SS variants. These findings suggest that environmental stress can significantly enhance the tolerance, survival, and persistence of S. enterica. Full article

During high-speed flight, intense friction on the aircraft surface always occurs due to atmospheric fluid medium. The resultant high frictional drag will trigger a significant aerothermal effect, and thus raise the surface temperature sharply to 1000–3000 °C. This extreme heat not only remarkably reduces the aerodynamic efficiency but probably also causes thermal failure of the structural integrity and damage of internal components. Therefore, robust heat-resistant materials are the preferred choice for designing high-speed aircraft due to their benign tolerance to high temperature, oxidation and ablation as well as large strength and durability. This work systematically unveils the generation mechanism of frictional drag in high-speed flight and introduces the characteristics and applications of typical thermal insulation materials (TIMs). After that, the recent progress in a thermally protected material system including metal-based alloys and metal-doped compound materials, ultra-high-temperature ceramics (UHTCs), carbon (C)/carbon (C) and C/SiC composites, ceramic matrix composites (CMCs), UHTCs-modified C/C and C/SiC composites is conducted. Finally, the current technical bottlenecks are discussed, simultaneously proposing the development direction of novel TIMs for the potential applications for high-speed aircrafts. Full article

Extreme weather events such as higher temperatures, droughts, and soil salinization are projected to increase as atmospheric CO₂ concentrations rise and climate change progresses. These factors have a negative impact on global food security, the water supply, and ecosystem productivity. The focus of this review is on modern concepts, comparative studies, and our data on the mechanisms of adaptation of halophytes and glycophytes with different types of photosynthetic metabolism (C₃, C₄) to the individual and combined effects of climatic factors. The analysis revealed that C₃ and C₄ species and C₄-NAD-ME and C₄-NADP-ME species differ in terms of stability and photosynthetic plasticity. Under drought conditions, both individually and in combination with other factors, C₄ halophytes demonstrate the advantages of efficient photosynthesis and salt tolerance. Halophytes with C₄-NADP-ME are characterized by uniquely high levels of plasticity and variability in photosynthetic metabolism. This is reflected in their ability to mitigate the negative effects of elevated temperatures and drought through the use of elevated CO₂ (eCO₂). The mitigating effect of eCO₂ on photosynthesis at elevated temperatures was not detected in halophytes, regardless of photosynthesis type. Halophytes possess an augmented capacity for heat tolerance. Integrating fundamental scientific knowledge with urgent practical needs will enable us to predict changes in ecosystems and create new, sustainable agricultural systems. Full article

In this work, we propose an incremental pulse-width erase (IPWE) scheme for fast and variation-tolerant gate-induced drain leakage (GIDL) erase of 3D NAND flash. For the GIDL erase operation, GIDL-generated hole accumulation is required to raise the channel potential. This requirement leads to a transient state that degrades erase speed and broadens distribution of the erased Vth. In addition, the degradation becomes more pronounced with critical-dimension (CD) variation and temperature variation. The proposed IPWE scheme increases erase pulse width progressively, rather than increasing erase voltage as in the conventional incremental step pulse erase (ISPE) scheme. Sentaurus TCAD simulations of a 3D NAND flash with a surrounded BL PAD structure demonstrate that the IPWE scheme achieves a 1.18 V larger Vth shift compared to the ISPE scheme for the same total erase time of 6.6 ms. The IPWE scheme also effectively narrows the erase Vth shift distribution, reducing it by 40 mV under a 55 nm CD variation, 0.26 V for a 10 nm CD variation between channel strings, and 2 V across a 50 K temperature variation, all within a total erase time of 6.6 ms. Full article

Botswana’s semi-arid climate makes it vulnerable to climate change, particularly drought, which threatens agricultural productivity. This study assesses drought impact on Botswana’s agricultural sector using Climate Hazards Center Infrared Precipitation with Station (CHIRPS) rainfall data and Climate Hazards Center Infrared Temperature with Station (CHIRTS) temperature data (25 km) to compute the Standardized Precipitation Index (SPI), Standardized Temperature Condition Index (STCI) and Standardized Precipitation Evapotranspiration Index (SPEI) at seasonal/annual time scales (1, 3, 6 and 12 months). The indices are used to assess their ability to predict crop yields using national data during Botswana’s rainy season, while employing univariate and multivariate statistical models. Statistical models also linked historical drought patterns to yield variability with the Percentage Area Affected (PAA) by drought, identifying key predictors. A majority of the crops (sunflower, maize, sorghum and pulses) showed variability which was best explained by SPEI 6 more particularly under the PAA multivariate models, with the highest and moderate explanatory power (R²) found in sunflower (0.48) and maize (0.43). However, variability in millet was best explained by SPI-3, although the R² was low (0.26). Other crops displayed positive coefficients within the models, which may be attributed to the varieties grown being drought tolerant. Nevertheless, the impacts from drought, which resulted in low yields, were shown by the negative coefficients across most crops. For a more holistic approach, the study also employed questionnaire data to capture first-hand local knowledge. The results showed drought to be among the indicators of climate change that were mostly perceived as well as its effects, in which yield decline, crop damage and crop pests and diseases were among the most perceived effects. Overall, this highlighted the sector’s vulnerability to the changes in climate. The study therefore underscores the need for integrated drought early warning systems, adaptive agricultural/water management and insights for policymakers to enhance drought resilience in Botswana, aligning with global sustainability goals. Full article

Primula vulgaris possesses considerable edible, medicinal, and ornamental value. It is widely applied in food and pharmaceutical development and, as an early-spring flowering plant, is used in landscaping. However, its range of applications and scope are significantly limited due to its inability to withstand high temperatures. This study aimed to investigate the heat tolerance of P. vulgaris under natural high temperatures during summer, identify the most heat-resistant varieties, and determine the optimal conditions for summer outdoor cultivation. Eight P. vulgaris varieties were selected and placed under forest shade with three different shading rates during the summer high-temperature period. Additionally, the heat damage index and the following six physiological indicators were measured: malondialdehyde (MDA) content, superoxide dismutase (SOD) activity, peroxidase (POD) activity, soluble sugar content, soluble protein content, and relative conductivity. Furthermore, a correlation analysis of the physiological indicators was conducted, and a heat tolerance evaluation was performed using the membership function method. Simultaneously, qRT-PCR was employed to analyze the expression patterns of three heat stress-related genes (PvHSP70, PvNCED6, and PvHSF24) across the different cultivars and experimental sites. Under heat stress conditions, leaf area was found to be positively and highly significantly correlated with stomatal density (p < 0.01). The heat damage index, MDA content, and relative conductivity increased significantly with prolonged stress, and they showed highly significant positive correlations. SOD activity, soluble sugar content, and soluble protein content increased to resist heat damage, while POD activity exhibited no consistent trend. Highly significant positive correlations were observed among protective enzyme activities and osmotic regulatory substances. After a comprehensive evaluation, the eight varieties were ranked according to heat tolerance as follows: “Early Punas Yellow” > “Danova Red” > “Middle Punas Rose Red” > “Middle Punas Blue” > “Middle Punas Red” > “Danova Rose White” > “Middle Punas Crimson” > “Middle Punas Scarlet”. Conclusions: “Early Punas Yellow”, “Danova Red”, and “Middle Punas Rose Red” demonstrated strong heat tolerance. In addition, the expression of PvHSP70 and PvHSF24 was significantly upregulated in heat-tolerant cultivars, while that of PvNCED6 showed a sustained increasing trend with rising temperatures. The results of a three-way ANOVA suggested that P. vulgaris exhibited different regulatory patterns among various traits under natural high-temperature stress. Morphological and integrative damage-related indicators, including leaf area, stomatal density, and the heat damage index, all presented significant “site × time” interaction effects. Meanwhile, some physiological regulatory indicators displayed more complex and inconsistent response patterns. These findings further confirm that a dense forest understory grassland is an ideal environment for the summer outdoor cultivation of P. vulgaris. Full article

Zonal ship central cooling systems, which are primarily implemented in naval platforms and advanced specialized vessels to ensure high survivability, exhibit complex fluid–thermal interactions and multi-level valve networks, challenging conventional resilience analysis, especially under large-scale fault scenarios and dynamic topology reconfiguration. This paper presents a physics-informed dynamic resilience assessment and reconfiguration optimization method tailored for such systems. To address the high-dimensional reconfiguration search space, a physics-informed pruning mechanism combining topological reachability filtering and nodal continuity-based feasible-flow verification is introduced, eliminating 42.6% of invalid topologies and reducing optimization time by approximately 38%. Additionally, a cumulative thermal severity (CTS) metric is developed to capture transient thermal shock risks, quantitatively assessing deviation from the 50 °C system safety boundary at the most critical node. Simulation results for a main seawater pump failure scenario demonstrate that the proposed reconfiguration strategy, which coordinates cross-zone tie valves and leverages healthy zones’ pressure margins, shortens recovery time by 47%, suppresses peak temperature from 51.5 °C to 50.2 °C, reduces maximum over-temperature from 1.5 °C to 0.2 °C, and decreases CTS from 8.5 °C·s to 0.1 °C·s (a 98.8% reduction). These findings demonstrate that physics-informed pruning substantially reduces the computational burden of high-dimensional reconfiguration, while the proposed CTS metric enables quantitative assessment of transient thermal-shock risk. Together, they offer robust methodological guidance for resilience-oriented decision support and fault-tolerant design in complex shipboard fluid–thermal systems. Full article

Heat stress is a major constraint to global egg production, as rising temperatures increasingly challenge the physiological limits of commercial layer chickens. This review integrates current advances in stress physiology to demonstrate that heat stress is not merely a thermoregulatory problem but a multi-systemic disruption involving neuroendocrine overload, metabolic imbalance, oxidative damage, immune suppression, and gastrointestinal barrier breakdown. These interacting pathways collectively impair egg production, shell quality, feed efficiency, and hen welfare. The review also synthesizes emerging mitigation strategies across environmental control, nutritional interventions, genetic and breeding innovations, welfare-oriented housing systems, and precision monitoring technologies. Studies indicate that targeted cooling, antioxidant, and electrolyte supplementation, the selection of heat-tolerant strains, enriched environments, and sensor-based early-warning systems can significantly enhance egg-laying hen resilience. Remaining gaps include a limited understanding of multi-stressor interactions, microbiome-mediated thermal tolerance, and the large-scale implementation of precision tools. The review provides a forward-looking framework for improving heat resilience in modern layer systems. Full article

Walnut (Juglans regia) is an economically significant woody oil tree species widely cultivated in China. However, its production is increasingly threatened by extreme low-temperature events, such as unseasonal frosts and late-spring cold. Salicylic acid (SA) is a key phytohormone known to enhance cold tolerance in plants, yet its underlying mechanism in walnut remains unclear. In this study, we present the first integrated analysis combining physiological measurements, transcriptomics, and metabolomics to investigate how exogenous SA improves cold tolerance in walnut leaves. Our results showed that SA treatment significantly increased the accumulation of soluble sugars, chlorophyll, and proline, enhanced peroxidase (POD) activity, and reduced malondialdehyde (MDA) levels under cold stress. Multi-omics analysis revealed that SA modulated the expression of genes involved in multiple hormone signaling pathways, including those of SA, auxin, jasmonic acid, and abscisic acid, and altered corresponding hormone levels. Notably, carbohydrate metabolism emerged as a central pathway mediating SA-induced cold adaptation. Weighted gene co-expression network analysis (WGCNA) further identified several core candidate genes, such as JrTGA, JrPP2C, JrTPS, and JrBAM, which may play key roles in this process. Collectively, this study provides the first multi-omics perspective on the regulatory network underlying SA-enhanced cold tolerance in walnut. These findings offer both a theoretical and technical foundation for applying SA in cold-resistant walnut cultivation and contribute to the development of stress-resilient production strategies. Full article

Temperature and humidity are critical abiotic factors shaping the survival and adaptation of insect pests. However, the molecular mechanisms underlying high-temperature tolerance under contrasting humidity conditions remain poorly understood, particularly in globally invasive species such as the tomato pinworm, Tuta absoluta. Previous studies have examined individual stressors, leaving interactive thermo-hygrometric effects on gene expression and survival insufficiently resolved. Here, we assessed the contribution of cytochrome P450 genes to thermal adaptation under low- and high-humidity conditions using transcriptome profiling combined with nanocarrier-mediated RNA interference (RNAi). Third-instar larvae were exposed to high temperature under low humidity (HT-LH: 40 °C, 50% RH) or high humidity (HT-HH: 40 °C, 75% RH) for eight hours. Survival declined from 97.5% in the control to 74.16% under HT-LH and 68.33% under HT-HH conditions. Transcriptome analysis revealed extensive differential gene expression, with 464 genes upregulated and 565 downregulated in HT-LH, and 1145 upregulated and 1166 downregulated in HT-HH. Functional annotation highlighted pathways linked to metabolic regulation, proteostasis, and detoxification, including multiple cytochrome P450-associated processes. RT-qPCR confirmed the upregulation (3–5 fold) of four P450 genes (CYP6AB327, CYP6ABF1b, CYP6AE214, and CYP9A306c) under high temperature across both humidity regimes. RNAi-mediated silencing of these genes significantly reduced larval survival, demonstrating their functional role in thermal-hygrometric stress tolerance across. Cytochrome P450 genes underpin the adaptive capacity of the tomato pinworm to high-temperature stress across contrasting humidity conditions, highlighting RNAi-based disruption of P450 function as a promising avenue for sustainable pest management under climate change scenarios. Full article