Search Results (1,356)

Return-type cable suspension bridges transfer the main-cable force to the anchorage predominantly in the horizontal direction, which may induce coupled sliding–overturning instability of the anchorage–foundation system. This study examines the stability of return-type cable gravity anchorage using the composite anchorage of the Jixin Expressway Yellow River Three Gorges Bridge as the prototype. A 1:100 laboratory specimen was designed based on similarity theory and tested under incremental loading until failure. Four configurations were considered by combining two embedment ratios (1/4 and 1/2) with two base types (flat-base and shear-keyed). Horizontal displacement, overturning angle, interface contact stress, and foundation strain were monitored throughout loading. Because the return-type cable transmits a predominantly horizontal force, the anchorage–foundation contact stress exhibits pronounced asymmetry between the toe and heel regions, and this stress asymmetry governs the coupled sliding–overturning instability mode. The shallow flat-base case exhibited a distinct displacement and contact stress jump at high load levels, followed by rapid rotation, indicating slip–tilt coupled instability. Increasing embedment improved confinement and delayed the onset of nonlinear deformation, but the flat-base configuration still showed pronounced toe stress concentration. By contrast, the shear-keyed base mobilized cooperative bearing of the surrounding foundation, producing smoother stress–strain evolution and higher ultimate capacity. Moreover, the shear-keyed base mitigates the stress asymmetry at the anchorage–foundation interface, leading to a more symmetric distribution of contact pressure and improved overall stability. Three-dimensional finite-element simulations reproduced the measured trends in displacement, stress concentration near the toe, and strain development, providing independent verification. The results clarify the dominant instability mechanism of return-type cable gravity anchors and offer design implications for embedment depth and shear-keyed base detailing. Full article

The activated sludge process, along with its modifications, is currently the most widely used wastewater treatment method to achieve desired environmental outcomes. However, it is also characterized by operational instability resulting from changing conditions, a wide range of quantitative and qualitative wastewater parameters, and technical and technological factors. Multi-parameter analysis of biological processes enables more comprehensive control through the use of chemometric techniques, modeling, artificial neural networks, and AI in the decision-making process. This article presents the results of a multivariate data analysis of parameters of activated sludge suspension held under anaerobic conditions. Several correlations were identified between parameters characterizing activated sludge and sludge liquid. PCA and HCA analyses enabled the extraction of three sets of parametric clusters. They reflect specific stages of sludge transformation under anaerobic conditions: initial high biological activity (cluster I), degradation and nutrient release (cluster II), and stabilization with minimal sludge activity (cluster III). These clusters indicate characteristic qualitative changes in sludge and sludge liquid, which can serve as effective control and optimization tools for biological wastewater treatment processes. Statistical and chemometric analyses demonstrate the potential to rapidly assess the condition of activated sludge or the stage of anaerobic transformation by correlating individual parameters. This is an example of how these tools can be used to control wastewater treatment processes more effectively, including in anaerobic conditions. Such control may improve treatment quality and the energy efficiency of the process. It will also help reduce the impact of treatment plants on the aquatic environment and enable the reuse of wastewater that is more effectively treated, which is undoubtedly an important element of sustainable development. Full article

This study describes the development of an electrochemical sensor for quantitatively measuring acetaminophen (ACOP) in drug tablets. The sensor design is based on the modification of glassy carbon electrode (GCE) using zinc oxide nanoparticles (ZnONPs) embedded in a naturally occurring clay matrix (Sa) functionalized with phenylalanine (Phe). To ensure that the ZnONPs are homogeneously dispersed on the clay surface, the nanocomposite was synthesized using an impregnation approach and low-temperature heat treatment. The amino acid promotes specific interactions with ACOP through hydrogen bonding and π-π stacking, acting as both a stabilizing agent and a molecular recognition moiety. FTIR, UV-Vis, XRD, and FESEM/EDX mapping were employed to fully characterize the developed material (ZnONPs-Sa/Phe). Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) were used for the electrochemical determination of ACOP using the modified electrode GCE/ZnONPs-Sa/Phe. Parameters susceptible to affecting the sensitivity of the developed sensor were optimized, revealing that 5 µL of the suspension ZnONPs-Sa/Phe immobilized on GCE was ideal for the sensing of ACOP in a phosphate buffer solution at pH 2.0. The calibration curve obtained by plotting peak current intensity against ACOP concentration exhibited linear behavior within the concentration range between 0.02 µM and 0.28 µM, enabling determination of the limits of detection (LOD) and quantitation (LOQ) at 8.54 × 10⁻⁹ M and 2.84 × 10⁻⁸ M, respectively. The reproducibility, stability, and selectivity of the sensor were evaluated, followed by its application to the nano-sensing of ACOP in Africure and Doliprane tablets, yielding satisfactory results. The simplicity, affordability, and high analytical sensitivity of the developed sensor make this sensing platform a promising tool for pharmaceutical quality control applications. Full article

Graphene possesses exceptional mechanical strength, high electrical conductivity, and a stable lattice structure, making it an ideal material for sensors in advanced manufacturing. However, these sensors face stability challenges due to complex electromagnetic interference (EMI) environments generated by electrical equipment. Therefore, investigating the influence of EMI on sensor performance is of significant importance. In this study, simulations were performed to analyze electrical parameter perturbations of intrinsic graphene films under EMI conditions. The Magnetic Fields, Solid Mechanics, and Electrostatics modules in COMSOL Multiphysics were employed to construct a coupled model of a three-phase power transformer and a graphene-based pressure sensor. The results indicate that EMI can induce baseline drift on the order of ~5% full scale (FS) in the graphene current density, accompanied by degradation in signal-to-noise ratio (SNR) exceeding ~15 dB under typical simulation conditions. Graphene in direct contact with metal electrodes shows enhanced sensitivity to EMI, with more pronounced noise amplification due to interfacial coupling effects. In contrast, cavity-suspended graphene configurations exhibit relatively improved robustness, suggesting that suspended membrane architectures can mitigate EMI by reducing parasitic coupling and enhancing mechanical isolation. Compared with previous studies, this work highlights the role of multiphysics coupling and membrane suspension in influencing EMI-induced perturbations, providing theoretical guidance for the design of graphene-based sensors in power system and industrial Internet of Things (IoT) applications. Full article

Luminescent organic–inorganic Cu(I) halide hybrid molecular crystals exhibit remarkable structural diversity and photophysical properties, but their application in aqueous environments is often limited by insufficient stability. Herein, we report portable and reusable photoluminescent sensors based on Cu(I)–I triethylenediamine derivatives [Cu₄I₆(pr-ted)₂] and [Cu₃I₅(bz-ted)₂] (pr-ted = 1-propyl-1,4-diazabicyclo[2.2.2]octan-1-ium; bz-ted = 1-benzyl-1,4-diazabicyclo[2.2.2]octan-1-ium). Their submicrometric particles exhibit intense UV-excited emissions and high photoluminescence quantum yields but limited water stability. To address this limitation, ultrasound sonication was employed to control particle size and produce stable suspensions that can be incorporated into polymeric matrices via 3D printing with photocurable resins or polylactic acid (PLA) films by drop-casting, yielding mechanically robust composites that retain their structural and optical properties. The devices used act as selective turn-off luminescent sensors for Fe³⁺ in aqueous media, with nanomolar detection limits (1.33–1.58 nM) below regulatory thresholds for drinking water. Moreover, [Cu₃I₅(bz-ted)₂] enables tetracycline detection in river water with a limit of detection of 0.038 nM. Mechanistic studies indicate that reversible photoinduced electron transfer is the primary quenching pathway, while composites maintain sensing performance over multiple reuse cycles. Full article

Recent advancements in neuroproteomics have enabled detailed analysis of protein expression in the human brain, yet resolving synaptic dysfunction—a central feature of many neurological and psychiatric disorders—requires careful methodological consideration. Leveraging the high sensitivity of modern liquid chromatography-tandem mass spectrometry (LC-MS/MS), we evaluated the utility of whole-tissue lysates versus enriched synaptosome preparations for detecting synaptic protein signatures. First, we optimized and standardized a sample preparation protocol for frozen human gray matter (GM) by refining the suspension trapping (sTRAP) digestion method using thin human tissue sections. We accomplished low technical variation by minimizing sample handling and achieved a highly reproducible sample preparation workflow by rigorously applying standardization and randomization across dissection, processing, and LC-MS/MS runs. Second, comparative LC-MS/MS analysis showed that while whole-tissue lysates provide a high-throughput survey of the synaptic proteome, synaptosome isolation is required to investigate synapse-specific proteins to detect alterations at the terminal that are obscured in the soma. Because these methods offer distinct but synergistic levels of information, we recommend a tiered neuroproteomics strategy. This approach utilizes whole-tissue lysates for broad disease-associated screening and consistent quantification in large cohorts, followed by targeted synaptosome proteomics to provide a unique window of insight into synaptic composition and stability. This integrated workflow respects the biological necessity of spatial resolution while maintaining the reproducibility required for robust human brain proteomics. Furthermore, initial tissue-level analysis provides the necessary context to correctly interpret synaptosome data in cases of global synapse loss or gain. Full article

Soil salinity is a major abiotic stressor that constrains plant growth and development, yet the coordinated regulatory mechanisms underlying salt stress impacts on plant cell mechanical properties and the cytoskeleton remain elusive. In this study, tobacco suspension cells were employed as a model system. Combining mechanical measurements, fluorescence microscopy imaging, and bright-field morphological observation, we systematically characterized the dynamic response patterns of cell-generated surface stress (ΔS), cell viscoelastic index (CVI), microfilament cytoskeleton structure, as well as cell morphology and plasmolysis under NaCl stress ranging from 50 to 150 mmol/L. The results revealed three distinct response thresholds: 50 mmol/L NaCl treatment induced only transient ΔS fluctuations and mild plasmolysis, with no significant changes in CVI or microfilament fluorescence intensity, suggesting a safe tolerance threshold. The 75–100 mmol/L NaCl treatments triggered reversible “rise–recovery” mechanical responses in ΔS and CVI. The microfilament cytoskeleton showed minor structural adjustments, and plasmolysis increased gradually but remained reversible, defining this range as a reversible acclimation phase. The 125–150 mmol/L NaCl treatment caused an irreversible decline in ΔS (with a sharp instantaneous drop at 150 mmol/L). CVI variations diminished and stabilized after 6 h. The microfilament cytoskeleton suffered progressive disruption, as fluorescence intensity dropped to 1% of the control group at 150 mmol/L, accompanied by severe plasmolysis and protoplast shrinkage, indicating irreversible cellular damage. These findings demonstrate a concentration-dependent gradient effect of NaCl stress, highlighting tight coordination between mechanical properties, cytoskeletal integrity, and morphological adaptation. This work provides critical cytological insights into the molecular regulation of plant salt stress responses. Full article

To address the pronounced self-aggregation of highly loaded silica in the aqueous phase and the substantial filler loss occurring during the flocculation stage of latex compounding, this study introduces disaggregated and activated sepiolite possessing a spatial confinement effect as both a suspension stabilizer and a synergistic reinforcing component. On this basis, a multiscale natural rubber (NR)/silica/sepiolite composite system was constructed via a latex compounding route. Rheological characterization combined with static sedimentation observations revealed that the percolation threshold of the sepiolite is approximately 0.8 wt%. When the sepiolite content exceeds 1.0 wt%, its fibrous morphology enables the formation of a continuous three-dimensional network, which physically constrains silica particles and effectively suppresses their sedimentation and self-aggregation in the aqueous medium. Guided by this percolation behavior, a stable silica/sepiolite hybrid slurry was subsequently wet-mixed with natural rubber latex, and the influence of sepiolite loading on silica retention during flocculation, as well as on the resulting composite properties, was systematically examined. The results demonstrate that incorporation of sepiolite reduces filler loss during flocculation, with the loss rate decreasing from 4.7% to 1.1%. The Payne effect, SEM, dynamic and static mechanical analyses indicate that an appropriate sepiolite dosage promotes dispersion of silica within the rubber matrix while simultaneously strengthening filler–rubber interfacial interactions. Accordingly, tensile and tear strengths are increased from 32.1 to 35.5 MPa and from 92.3 to 133.4 N·mm⁻¹, respectively, while wet skid resistance is preserved and both rolling resistance and wear resistance are further improved. The findings of this work establish a practical and efficient strategy for the wet preparation of high-performance NR/silica composites. Full article

Deep soft-rock tunnels are prone to large deformations and structural damage. This study used the Guanyinping Tunnel as a prototype and conducted 1/50-scale progressive loading model tests under three support configurations: rock-bolt-free, equal-length rock bolts, and mixed long–short rock bolts. Rock stress, radial rock displacement (u), and rock bolt axial force (F_N) at the vault, arch shoulders, sidewalls, and wall feet were monitored to reveal reinforcement mechanisms and mechanical response. The results indicated that stress evolution in the bolt-free case exhibited significant spatial heterogeneity. The vault experienced horizontal stress concentration, while the arch shoulder underwent vertical stress concentration. u underwent a three-stage nonlinear progression: elastic linear growth, plastic linear growth, and plastic-accelerated growth. Displacement at the vault was markedly larger than that at other locations. Equal-length rock bolts substantially improved the rock mass stability by delaying stress concentration and fracture propagation. This reinforcement raised the elastic response threshold to 96 kPa and substantially reduced u. F_N at the vault and shoulder followed linear growth, accelerated growth, and then gradual decline, whereas F_N at the sidewalls and wall feet exhibited a steady linear trend. Combined long and short rock bolts produced a multi-level anchoring effect. Short bolts induced a shallow arching action, while long bolts provided deep suspension. This synergy raised the elastic response threshold to a maximum of 120 kPa and moderated the stress reduction process. Deep residual stresses increased to 74.3–88.4% of peak values. The displacement gradient between shallow and deep rock masses was significantly reduced. The coordinated deformation capacity within the anchoring zone was markedly enhanced. F_N distribution exhibited spatial differentiation: short bolts carried the load initially, followed by the activation of long bolts. Both anchoring schemes increased residual stress and mitigated rock mass deformation. The deformation control effect was stronger in shallow rock mass than in deep rock mass. Improvements at the vault and arch shoulders exceeded those at the sidewalls and wall feet. The mixed short–long bolt configuration was superior because it maximized the self-bearing capacity of the deep rock mass. The findings provide experimental data and theoretical guidance for the design and optimization of rock-bolt support in deep soft-rock tunnels. Full article

Subcutaneous administration has become an increasingly important route for delivering protein therapeutics, driven by patient convenience and the growing use of self-administration devices. However, conventional subcutaneous injection systems are typically limited to injection volumes of approximately 1–2 mL, posing significant formulation challenges for protein drugs requiring high therapeutic doses. Monoclonal antibodies (mAbs), for example, often require concentrations exceeding 100 mg/mL to enable subcutaneous delivery, which introduces challenges related to limited solubility, elevated viscosity, and an increased risk of physical and chemical instability. Therefore, high-concentration protein suspensions have emerged as a promising formulation strategy to overcome these limitations and enable subcutaneous administration of high-dose proteins. In such systems, therapeutic protein solid particles are suspended in vehicles in which they are insoluble, giving rise to unique considerations related to particle properties, protein stability, and suspension behaviors such as viscosity, injectability, and sedimentation. Accordingly, multiple particle production approaches have been explored to enable the development of ultra-high-concentration protein suspensions (>200 mg/mL). This review article aims to provide a comprehensive overview of particle formation techniques and the relationships between key particle properties and suspension performance attributes relevant to the development of high-concentration protein suspensions for injectable applications, as well as future directions in this field. Full article

The safe release of external stores from aircraft is a complex aerodynamic problem governed by strong interactions between the store and the carrier. During separation, the store is subjected to rapidly varying pressure fields, strong aerodynamic interference, and inertial effects that collectively determine the trajectory and stability of the body in the critical milliseconds following release. This study presents a numerical investigation of the separation of an external store from the high-wing configuration aircraft. Both gravitational and impulse-based release mechanisms are examined across multiple suspension stations and a wide range of flight conditions. Computational fluid dynamics (CFD) methods were employed using a density-based, compressible solver with SST k–ω turbulence modeling, combined with a fully coupled six-degree-of-freedom (6DOF) solver and dynamic mesh deformation techniques. The study considers a wide range of Mach numbers from 0.6 to 0.9 and angles-of-attack between −2° and 4°, and three different suspension stations located at the inner wing pylon, outer wing pylon, and fuselage centerline. These conditions strongly influence the aerodynamic environment around the store and therefore affect its initial motion after release and flight path. The impulse ejection forces used in the analysis come from experimental data and are applied through a user-defined function (UDF) at each time step, allowing the simulation to reproduce the ejection event as realistically as possible. Numerical results confirm that the flight paths of external store are highly non-symmetrical, requiring the employment of complex computational models for their successful resolution, and that they gravely depend on the operating conditions, carrier geometry as well as the suspension location. Full article

The construction of suspension bridges in mountainous expressways often involves tunnel-type anchorages in close proximity to shallow-buried bifurcated tunnels, particularly in soft rock strata with dense overlying structures. This proximity poses significant challenges to construction safety and stability. This study aims to investigate the influence of tunnel-type anchorage construction on the ground surface, surrounding rock, and adjacent bifurcated tunnels under such complex conditions. It was hypothesized that the anchorage load transfer and deformation mechanisms would significantly affect the adjacent tunnel, with potential cumulative effects due to the twin-anchor configuration. To address this, a combined approach of in situ scaled model testing (1:10 scale) and three-dimensional numerical simulation was employed. The model test incorporated monitoring of deformation and stress at key locations (anchor plug, rock mass, and anchor–rock interface) under incremental cable loads. Quantitative results from the model test indicate that at the design load (1P, equivalent to 2016.84 kN per anchor), deformations were minimal (e.g., maximum anchor displacement 0.35 mm). The anchor–rock interface exhibited limited slip (max 0.06 mm at 1P), and contact stresses were highest in the rear part of the anchor plug, indicating a non-uniform load transfer. Under overload conditions, the system reached yield at 7P and peak strength at 10.5P, with measured ground surface cracks up to 5 mm. Numerical simulations, calibrated against the experimental data, revealed that under increasing load (up to 10P), the plastic zones around the two anchors progressively expanded and eventually coalesced, leading to a characteristic “inverted trapezoid” failure pattern propagating to the surface, accompanied by shear failure along the 14° bedding plane. The combined results quantify the progressive interaction between the twin anchorages and the surrounding rock, highlighting the critical role of the anchor–rock interface and the cumulative effect of twin anchors on ground deformation and potential failure mechanisms. This research provides a scientific basis for the design and construction of tunnel-type anchorages in similar challenging geological and spatial settings. Full article

This study aimed to establish a more sensitive and reliable quantitative method for determining rafoxanide (RFX) in ovine plasma. The method was applied in an 84-day long-term comparative pharmacokinetic trial to evaluate the performance of a rafoxanide nanosuspension (RFX-NS) versus its conventional suspension (RFX-S) in sheep. Characterization of the two formulations revealed the following results: RFX-NS had an average particle size of 484.93 ± 43.11 nm, a uniform size distribution (PDI 0.06 ± 0.07), and a Zeta potential of −43.59 ± 0.67 mV, which were significantly superior to those of RFX-S (particle size 2379.67 ± 121.71 nm, PDI 0.93 ± 0.10, Zeta potential −38.10 ± 0.55 mV), demonstrating excellent physical stability. In vitro dissolution tests indicated a higher dissolution rate for RFX-NS (97.6% at 60 min). By integrating formulation characterization, in vitro dissolution assessment, and pharmacokinetic studies, this research provided a comprehensive analysis of the differences between the two formulations. Pharmacokinetic results showed that, compared to RFX-S, RFX-NS had a significantly reduced apparent volume of distribution (Vz/F: 856.02 ± 274.00 vs. 1404.17 ± 285.1 mL/kg, p < 0.01), a shorter elimination half-life (t_1/2: 4.05 ± 1.18 vs. 5.32 ± 0.94 days, p < 0.05), and a relative bioavailability of 128.98%. These findings suggest that RFX-NS is an improved novel formulation superior to the conventional suspension, as it enhances drug efficacy and efficiency by improving drug dissolution and absorption. Full article

Active suspensions in automotive applications are designed to improve vehicle stability and comfort and reduce vibration transmission from the road surface. Active systems often include a dedicated actuator, and, to reduce their mass and energy absorption, it is a typical choice to rely on brushless electric motors with permanent magnets containing Critical Raw Materials such as Neodymium, a Rare Earth Element (REE), offering favorable power density values. Although these systems offer clear advantages in terms of ride quality and performance, their direct and indirect energy requirements, combined with their dependence on resource-intensive materials, raise concerns about life cycle sustainability: in other words, there is a trade-off between production impact (relevant for REE) and use impact (reduced by REE adoption). To address this issue, the research proposes a method to estimate energy consumption during the use phase of a vehicle through a dedicated parametric modeling and simulation framework; the aim is to evaluate the energy performance of active suspension systems under different road and driving conditions. The analysis explores how design parameters and operational choices affect energy consumption and efficiency. The simulation results reveal a marked sensitivity of system performance to road profiles and driving scenarios, highlighting the importance of holistic assessments during the early stages of design. The proposed framework represents a first step toward integrating circular design principles into the development of active suspensions. By combining technical and environmental perspectives, it supports the development of next-generation automotive components that balance comfort, performance, and sustainability. Full article

Poly(glycidyl methacrylate) (PGMA) is a polymer containing epoxy groups in its side chains, which makes it a suitable platform for the development of functional materials. In this study, crosslinked PGMA-based microspheres were synthesized by suspension polymerization using N,N′-methylenebisacrylamide as a crosslinker, and the effect of incorporating inorganic additives (InAds) (NaCl, CaCO₃, and MgO nanoparticles) during synthesis was evaluated. In all cases, solid microspheres were obtained, exhibiting variations in particle size, sphericity, and aggregation depending on the type and amount of InAds. Thermal stability was characteristic of crosslinked PGMA (i.e., a single broad thermal transition in ~80–110 °C), while water absorption remained within a narrow range (80–120% for t = 40 min). In addition, the number of epoxy groups per gram of sample was 4.83 ± 0.02 mmol g⁻¹. Selected microspheres were subsequently functionalized with polyethyleneimine (PEI) to obtain graft polymers (PGMA–PEI) and evaluated for the adsorption of three model contaminants: Acid Red 27 (AR-27), nitrites, and acetaminophen. PGMA–PEI showed high affinity toward AR-27 and nitrites, achieving high removal efficiencies at acidic and neutral pH, with rapid adsorption kinetics consistent with a pseudo-second-order model, attributed to electrostatic interactions between protonated amine groups and anions. At pH 11, anion desorption was promoted, enabling partial material regeneration. The results highlight the potential of PGMA–PEI microspheres for the removal of AR-27 (maximum retention ~0.25 mg of dye/g of polymer) and nitrites (maximum retention ~0.023 mg of ${NO}_2^{-}$/g of polymer), whereas acetaminophen removal was not evidenced. Full article