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Search Results (15,572)

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25 pages, 8312 KiB  
Article
Quantitative Assessment of Woven Fabric Surface Changes During Martindale Abrasion Using Contactless Optical Profilometry
by Małgorzata Matusiak and Gabriela Kosiuk
Materials 2025, 18(15), 3636; https://doi.org/10.3390/ma18153636 (registering DOI) - 1 Aug 2025
Abstract
The abrasion resistance of fabrics is one of the basic properties determining the utility performance and durability. The abrasion resistance of textile materials is measured using the Martindale device according to appropriate standards. The sample breakage method is the most commonly used of [...] Read more.
The abrasion resistance of fabrics is one of the basic properties determining the utility performance and durability. The abrasion resistance of textile materials is measured using the Martindale device according to appropriate standards. The sample breakage method is the most commonly used of the three methods. The method is based on organoleptic assessment of fabric breakage. The method is time-consuming, and results may be subject to error resulting from the subjective nature of the assessment. The aim of the presented work was to check the possibility of the application of contactless 3D surface geometry measurement using an optical profilometer in an assessment of changes in fabrics’ surface due to the abrasion process. The obtained results confirmed that some parameters of the geometric structure of fabric surfaces, such as the highest height of the roughness profile Rz, the height of the highest pick of the roughness profile Rp, the depth of the lowest valley of the roughness profile Rv, the depth of the total height of the roughness profile Rt, and the kurtosis Rku, can be used to assess the abrasion resistance of fabrics. It is also stated that using the non-contact optical measurement of fabric surface geometry allows for an assessment of the directionality of surface texture. For this purpose, the autocorrelation function and angle distribution function can be applied. Full article
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10 pages, 1883 KiB  
Article
In Vitro Biofilm Formation Kinetics of Pseudomonas aeruginosa and Escherichia coli on Medical-Grade Polyether Ether Ketone (PEEK) and Polyamide 12 (PA12) Polymers
by Susana Carbajal-Ocaña, Kristeel Ximena Franco-Gómez, Valeria Atehortúa-Benítez, Daniela Mendoza-Lozano, Luis Vicente Prado-Cervantes, Luis J. Melgoza-Ramírez, Miguel Delgado-Rodríguez, Mariana E. Elizondo-García and Jorge Membrillo-Hernández
Hygiene 2025, 5(3), 32; https://doi.org/10.3390/hygiene5030032 (registering DOI) - 1 Aug 2025
Abstract
Biofilms, structured communities of microorganisms encased in an extracellular matrix, are a major cause of persistent infections, particularly when formed on medical devices. This study investigated the kinetics of biofilm formation by Escherichia coli and Pseudomonas aeruginosa, two clinically significant pathogens, on [...] Read more.
Biofilms, structured communities of microorganisms encased in an extracellular matrix, are a major cause of persistent infections, particularly when formed on medical devices. This study investigated the kinetics of biofilm formation by Escherichia coli and Pseudomonas aeruginosa, two clinically significant pathogens, on two medical-grade polymers: polyether ether ketone (PEEK) and polyamide 12 (PA12). Using a modified crystal violet staining method and spectrophotometric quantification, we evaluated biofilm development over time on polymer granules and catheter segments composed of these materials. Results revealed that PEEK surfaces supported significantly more biofilm formation than PA12, with peak accumulation observed at 24 h for both pathogens. Conversely, PA12 demonstrated reduced bacterial adhesion and lower biofilm biomass, suggesting surface characteristics less conducive to microbial colonization. Additionally, the study validated a reproducible protocol for assessing biofilm formation, providing a foundation for evaluating anti-biofilm strategies. While the assays were performed under static in vitro conditions, the findings highlight the importance of material selection and early prevention strategies in the design of infection-resistant medical devices. This work contributes to the understanding of how surface properties affect microbial adhesion and underscores the critical need for innovative surface modifications or coatings to mitigate biofilm-related healthcare risks. Full article
(This article belongs to the Section Hygiene in Healthcare Facilities)
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31 pages, 711 KiB  
Review
Persistent Threats: A Comprehensive Review of Biofilm Formation, Control, and Economic Implications in Food Processing Environments
by Alexandra Ban-Cucerzan, Kálmán Imre, Adriana Morar, Adela Marcu, Ionela Hotea, Sebastian-Alexandru Popa, Răzvan-Tudor Pătrînjan, Iulia-Maria Bucur, Cristina Gașpar, Ana-Maria Plotuna and Sergiu-Constantin Ban
Microorganisms 2025, 13(8), 1805; https://doi.org/10.3390/microorganisms13081805 (registering DOI) - 1 Aug 2025
Abstract
Biofilms are structured microbial communities that pose significant challenges to food safety and quality within the food-processing industry. Their formation on equipment and surfaces enables persistent contamination, microbial resistance, and recurring outbreaks of foodborne illness. This review provides a comprehensive synthesis of current [...] Read more.
Biofilms are structured microbial communities that pose significant challenges to food safety and quality within the food-processing industry. Their formation on equipment and surfaces enables persistent contamination, microbial resistance, and recurring outbreaks of foodborne illness. This review provides a comprehensive synthesis of current knowledge on biofilm formation mechanisms, genetic regulation, and the unique behavior of multi-species biofilms. The review evaluates modern detection and monitoring technologies, including PCR, biosensors, and advanced microscopy, and compares their effectiveness in industrial contexts. Real-world outbreak data and a global economic impact analysis underscore the urgency for more effective regulatory frameworks and sanitation innovations. The findings highlight the critical need for integrated, proactive biofilm management approaches to safeguard food safety, reduce public health risks, and minimize economic losses across global food sectors. Full article
24 pages, 11098 KiB  
Article
Fracture Mechanisms of Electrothermally Fatigued 631 Stainless Steel Fine Wires for Probe Spring Applications
by Chien-Te Huang, Fei-Yi Hung and Kai-Chieh Chang
Appl. Sci. 2025, 15(15), 8572; https://doi.org/10.3390/app15158572 (registering DOI) - 1 Aug 2025
Abstract
This study systematically investigates 50 μm-diameter 631 stainless steel fine wires subjected to both sequential and simultaneous electrothermomechanical loading to simulate probe spring conditions in microelectronic test environments. Under cyclic current loading (~104 A/cm2), the 50 μm 631SS wire maintained [...] Read more.
This study systematically investigates 50 μm-diameter 631 stainless steel fine wires subjected to both sequential and simultaneous electrothermomechanical loading to simulate probe spring conditions in microelectronic test environments. Under cyclic current loading (~104 A/cm2), the 50 μm 631SS wire maintained electrical integrity up to 0.30 A for 15,000 cycles. Above 0.35 A, rapid oxide growth and abnormal grain coarsening resulted in surface embrittlement and mechanical degradation. Current-assisted tensile testing revealed a transition from recovery-dominated behavior at ≤0.20 A to significant thermal softening and ductility loss at ≥0.25 A, corresponding to a threshold temperature of approximately 200 °C. These results establish the endurance limit of 631 stainless steel wire under coupled thermal–mechanical–electrical stress and clarify the roles of Joule heating, oxidation, and microstructural evolution in electrical fatigue resistance. A degradation map is proposed to inform design margins and operational constraints for fatigue-tolerant, electrically stable interconnects in high-reliability probe spring applications. Full article
(This article belongs to the Special Issue Application of Fracture Mechanics in Structures)
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19 pages, 1259 KiB  
Article
Influence of Monosodium Glutamate on Astroglia of Rat Habenula
by Aleksandra Krawczyk, Karol Rycerz, Jadwiga Jaworska-Adamu and Marcin B. Arciszewski
Biomolecules 2025, 15(8), 1111; https://doi.org/10.3390/biom15081111 (registering DOI) - 1 Aug 2025
Abstract
The habenula (Hb) of the epithalamus is formed of the medial (MHb) and lateral (LHb) parts. The improper functioning of the Hb may lead to depression and anxiety. The glutamate excitotoxicity is accompanied by astroglia reactivity and leads to the damage of nervous [...] Read more.
The habenula (Hb) of the epithalamus is formed of the medial (MHb) and lateral (LHb) parts. The improper functioning of the Hb may lead to depression and anxiety. The glutamate excitotoxicity is accompanied by astroglia reactivity and leads to the damage of nervous system structures. The aim of the study was to assess the influence of monosodium glutamate (MSG) administrated subcutaneously to rats in doses of 2 g/kg b.w. (I) and 4 g/kg b.w. (II), on astroglia in the MHb and LHb. Based on immunohistochemical reactions, the morphology, number of astrocytes immunoreactive for glial fibrillary acidic protein (GFAP-IR) and S100β protein (S100β-IR), and their surface area, perimeter, number and length of processes, and cytoplasmic-nuclear immunostaining intensity for the studied proteins were assessed. In the MHb of animals receiving MSG, especially at a high dose, hypertrophy and an increase in the number of GFAP-IR and S100β-IR cells were demonstrated. In the LHb, only hypertrophy of processes in S100β-positive glia was observed. The immunostaining intensity increased in GFAP-IR glia and decreased in S100β-IR cells only in animals from group I. The results revealed that astroglia respond to MSG depending on its dose and the Hb part. This different behavior of glia may indicate their different sensitivity and resistance to damaging factors. Full article
12 pages, 1739 KiB  
Article
Tailored Levofloxacin Incorporated Extracellular Matrix Nanoparticles for Pulmonary Infections
by Raahi Patel, Ignacio Moyano, Masahiro Sakagami, Jason D. Kang, Phillip B. Hylemon, Judith A. Voynow and Rebecca L. Heise
Int. J. Mol. Sci. 2025, 26(15), 7453; https://doi.org/10.3390/ijms26157453 (registering DOI) - 1 Aug 2025
Abstract
Cystic fibrosis produces viscous mucus in the lung that increases bacterial invasion, causing persistent infections and subsequent inflammation. Pseudomonas aeruginosa and Staphylococcus aureus are two of the most common infections in cystic fibrosis patients that are resistant to antibiotics. One antibiotic approved to [...] Read more.
Cystic fibrosis produces viscous mucus in the lung that increases bacterial invasion, causing persistent infections and subsequent inflammation. Pseudomonas aeruginosa and Staphylococcus aureus are two of the most common infections in cystic fibrosis patients that are resistant to antibiotics. One antibiotic approved to treat these infections is levofloxacin (LVX), which functions to inhibit bacterial replication but can be further developed into tailorable particles. Nanoparticles are an emerging inhaled therapy due to enhanced targeting and delivery. The extracellular matrix (ECM) has been shown to possess pro-regenerative and non-toxic properties in vitro, making it a promising delivery agent. The combination of LVX and ECM formed into nanoparticles may overcome barriers to lung delivery to effectively treat cystic fibrosis bacterial infections. Our goal is to advance CF care by providing a combined treatment option that has the potential to address both bacterial infections and lung damage. Two hybrid formulations of a 10:1 and 1:1 ratio of LVX to ECM have shown neutral surface charges and an average size of ~525 nm and ~300 nm, respectively. The neutral charge and size of the particles may suggest their ability to attract toward and penetrate through the mucus barrier in order to target the bacteria. The NPs have also been shown to slow the drug dissolution, are non-toxic to human airway epithelial cells, and are effective in inhibiting Pseudomonas aeruginosa and Staphylococcus aureus. LVX-ECM NPs may be an effective treatment for pulmonary CF bacterial treatments. Full article
(This article belongs to the Special Issue The Advances in Antimicrobial Biomaterials)
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16 pages, 24404 KiB  
Article
Oxidation of HfB2-HfO2-SiC Ceramics Modified with Ti2AlC Under Subsonic Dissociated Airflow
by Elizaveta P. Simonenko, Aleksey V. Chaplygin, Nikolay P. Simonenko, Ilya V. Lukomskii, Semen S. Galkin, Anton S. Lysenkov, Ilya A. Nagornov, Artem S. Mokrushin, Tatiana L. Simonenko, Anatoly F. Kolesnikov and Nikolay T. Kuznetsov
Corros. Mater. Degrad. 2025, 6(3), 35; https://doi.org/10.3390/cmd6030035 (registering DOI) - 1 Aug 2025
Abstract
Ultrahigh-temperature ceramic composites based on hafnium diboride have a wide range of applications, including as components for high-speed aircraft and energy generation and storage devices. Consequently, developing methodologies for their fabrication and studying their properties are of paramount importance, in particular in using [...] Read more.
Ultrahigh-temperature ceramic composites based on hafnium diboride have a wide range of applications, including as components for high-speed aircraft and energy generation and storage devices. Consequently, developing methodologies for their fabrication and studying their properties are of paramount importance, in particular in using them as an electrode material for energy storage devices with increased oxidation resistance. This study investigates the behavior of ceramic composites based on the HfB2-HfO2-SiC system, obtained using 15 vol% Ti2AlC MAX-phase as a sintering component, under the influence of subsonic flow of dissociated air. It was determined that incorporating the modifying component (Ti2AlC) altered the composition of the silicate melt formed on the surface during ceramic oxidation. This modification led to the observation of a protective antioxidant function. Consequently, liquation was observed in the silicate melt layer, resulting in the formation of spherical phase inhomogeneities in its volume with increased content of titanium, aluminum, and hafnium. It is hypothesized that the increase in the high-temperature viscosity of this melt prevents it from being carried away in the form of drops, even at a surface temperature of ~1900–2000 °C. Despite the established temperature, there is no sharp increase in its values above 2400–2500 °C. This is due to the evaporation of silicate melt from the surface. In addition, the electrochemical behavior of the obtained material in a liquid electrolyte medium (KOH, 3 mol/L) was examined, and it was shown that according to the value of electrical conductivity and specific capacitance, it is a promising electrode material for supercapacitors. Full article
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46 pages, 4006 KiB  
Review
Solvent-Driven Electroless Nickel Coatings on Polymers: Interface Engineering, Microstructure, and Applications
by Chenyao Wang, Heng Zhai, David Lewis, Hugh Gong, Xuqing Liu and Anura Fernando
Coatings 2025, 15(8), 898; https://doi.org/10.3390/coatings15080898 (registering DOI) - 1 Aug 2025
Abstract
Electroless nickel deposition (ELD) is an autocatalytic technique extensively used to impart conductive, protective, and mechanical functionalities to inherently non-conductive synthetic substrates. This review systematically explores the fundamental mechanisms of electroless nickel deposition, emphasising recent advancements in surface activation methods, solvent systems, and [...] Read more.
Electroless nickel deposition (ELD) is an autocatalytic technique extensively used to impart conductive, protective, and mechanical functionalities to inherently non-conductive synthetic substrates. This review systematically explores the fundamental mechanisms of electroless nickel deposition, emphasising recent advancements in surface activation methods, solvent systems, and microstructural control. Critical analysis reveals that bio-inspired activation methods, such as polydopamine (PDA) and tannic acid (TA), significantly enhance coating adhesion and durability compared to traditional chemical etching and plasma treatments. Additionally, solvent engineering, particularly using polar aprotic solvents like dimethyl sulfoxide (DMSO) and ethanol-based systems, emerges as a key strategy for achieving uniform, dense, and flexible coatings, overcoming limitations associated with traditional aqueous baths. The review also highlights that microstructural tailoring, specifically the development of amorphous-nanocrystalline hybrid nickel coatings, effectively balances mechanical robustness (hardness exceeding 800 HV), flexibility, and corrosion resistance, making these coatings particularly suitable for wearable electronic textiles and smart materials. Furthermore, commercial examples demonstrate the real-world applicability and market readiness of nickel-coated synthetic fibres. Despite significant progress, persistent challenges remain, including reliable long-term adhesion, internal stress management, and environmental sustainability. Future research should prioritise environmentally benign plating baths, standardised surface activation protocols, and scalable deposition processes to fully realise the industrial potential of electroless nickel coatings. Full article
(This article belongs to the Section Surface Characterization, Deposition and Modification)
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14 pages, 6773 KiB  
Article
MoTiCo Conversion Coating on 7075 Aluminium Alloy Surface: Preparation, Corrosion Resistance Analysis, and Application in Outdoor Sports Equipment Trekking Poles
by Yiqun Wang, Feng Huang and Xuzheng Qian
Metals 2025, 15(8), 864; https://doi.org/10.3390/met15080864 (registering DOI) - 1 Aug 2025
Abstract
The problem of protecting 7075 Al alloy trekking poles from corrosion in complex outdoor environments was addressed using a composite conversion coating system. This system comprised Na2MoO4, NaF, CoSO4·7H2O, ethylenediaminetetraacetic acid-2Na, and H2(TiF [...] Read more.
The problem of protecting 7075 Al alloy trekking poles from corrosion in complex outdoor environments was addressed using a composite conversion coating system. This system comprised Na2MoO4, NaF, CoSO4·7H2O, ethylenediaminetetraacetic acid-2Na, and H2(TiF6). The influences of this system on the properties of the coating layer were systematically studied by adjusting the pH of the coating solution. The conversion temperature and pH were the pivotal parameters influencing the formation of the conversion coating. The pH substantially influenced the compactness of the coating layer, acting as a regulatory agent of the coating kinetics. When the conversion temperature and pH were set to 40 °C and 3.8, respectively, the prepared coating layer displayed optimal performance in terms of compactness and protective properties. Therefore, this parameter combination favours the synthesis of high-performance conversion coatings. Microscopy confirmed the formation of a continuous, dense composite oxide film structure under these conditions, effectively blocking erosion in corrosive media. Furthermore, the optimised process led to substantial enhancements in the environmental adaptabilities and service lives of the components of trekking poles, thus establishing a theoretical foundation and technical reference for use in the surface protection of outdoor equipment. Full article
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16 pages, 3282 KiB  
Article
First-Principles Study on Periodic Pt2Fe Alloy Surface Models for Highly Efficient CO Poisoning Resistance
by Junmei Wang, Qingkun Tian, Harry E. Ruda, Li Chen, Maoyou Yang and Yujun Song
Nanomaterials 2025, 15(15), 1185; https://doi.org/10.3390/nano15151185 (registering DOI) - 1 Aug 2025
Abstract
Surface and sub-surface atomic configurations are critical for catalysis as they host the active sites governing electrochemical processes. This study employs density functional theory (DFT) calculations and Monte Carlo simulations combined with the cluster-expansion approach to investigate atom distribution and Pt segregation in [...] Read more.
Surface and sub-surface atomic configurations are critical for catalysis as they host the active sites governing electrochemical processes. This study employs density functional theory (DFT) calculations and Monte Carlo simulations combined with the cluster-expansion approach to investigate atom distribution and Pt segregation in Pt-Fe alloys across varying Pt/Fe ratios. Our simulations reveal a strong tendency for Pt atoms to segregate to the surface layer while Fe atoms enrich the sub-surface region. Crucially, the calculations predict the stability of a periodic Pt2Fe alloy surface model, characterized by specific defect structures, at low platinum content and low annealing temperatures. Electronic structure analysis indicates that forming this Pt2Fe surface alloy lowers the d-band center of Pt atoms, weakening CO adsorption and thereby enhancing resistance to CO poisoning. Although defect-induced strains can modulate the d-band center, crystal orbital Hamilton population (COHP) analysis confirms that such strains generally strengthen Pt-CO interactions. Therefore, the theoretical design of Pt2Fe alloy surfaces and controlling defect density are predicted to be effective strategies for enhancing catalyst resistance to CO poisoning. This work highlights the advantages of periodic Pt2Fe surface models for anti-CO poisoning and provides computational guidance for designing efficient Pt-based electrocatalysts. Full article
(This article belongs to the Section Theory and Simulation of Nanostructures)
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21 pages, 3814 KiB  
Article
Features of the Structure of Layered Epoxy Composite Coatings Formed on a Metal-Ceramic-Coated Aluminum Base
by Volodymyr Korzhyk, Volodymyr Kopei, Petro Stukhliak, Olena Berdnikova, Olga Kushnarova, Oleg Kolisnichenko, Oleg Totosko, Danylo Stukhliak and Liubomyr Ropyak
Materials 2025, 18(15), 3620; https://doi.org/10.3390/ma18153620 (registering DOI) - 1 Aug 2025
Abstract
Difficult, extreme operating conditions of parabolic antennas under precipitation and sub-zero temperatures require the creation of effective heating systems. The purpose of the research is to develop a multilayer coating containing two metal-ceramic layers, epoxy composite layers, carbon fabric, and an outer layer [...] Read more.
Difficult, extreme operating conditions of parabolic antennas under precipitation and sub-zero temperatures require the creation of effective heating systems. The purpose of the research is to develop a multilayer coating containing two metal-ceramic layers, epoxy composite layers, carbon fabric, and an outer layer of basalt fabric, which allows for effective heating of the antenna, and to study the properties of this coating. The multilayer coating was formed on an aluminum base that was subjected to abrasive jet processing. The first and second metal-ceramic layers, Al2O3 + 5% Al, which were applied by high-speed multi-chamber cumulative detonation spraying (CDS), respectively, provide maximum adhesion strength to the aluminum base and high adhesion strength to the third layer of the epoxy composite containing Al2O3. On this not-yet-polymerized layer of epoxy composite containing Al2O3, a layer of carbon fabric (impregnated with epoxy resin) was formed, which serves as a resistive heating element. On top of this carbon fabric, a layer of epoxy composite containing Cr2O3 and SiO2 was applied. Next, basalt fabric was applied to this still-not-yet-polymerized layer. Then, the resulting layered coating was compacted and dried. To study this multilayer coating, X-ray analysis, light and raster scanning microscopy, and transmission electron microscopy were used. The thickness of the coating layers and microhardness were measured on transverse microsections. The adhesion strength of the metal-ceramic coating layers to the aluminum base was determined by both bending testing and peeling using the adhesive method. It was established that CDS provides the formation of metal-ceramic layers with a maximum fraction of lamellae and a microhardness of 7900–10,520 MPa. In these metal-ceramic layers, a dispersed subgrain structure, a uniform distribution of nanoparticles, and a gradient-free level of dislocation density are observed. Such a structure prevents the formation of local concentrators of internal stresses, thereby increasing the level of dispersion and substructural strengthening of the metal-ceramic layers’ material. The formation of materials with a nanostructure increases their strength and crack resistance. The effectiveness of using aluminum, chromium, and silicon oxides as nanofillers in epoxy composite layers was demonstrated. The presence of structures near the surface of these nanofillers, which differ from the properties of the epoxy matrix in the coating, was established. Such zones, specifically the outer surface layers (OSL), significantly affect the properties of the epoxy composite. The results of industrial tests showed the high performance of the multilayer coating during antenna heating. Full article
(This article belongs to the Section Metals and Alloys)
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25 pages, 9193 KiB  
Article
Antibiotic-Loaded Bioglass 45S5 for the Treatment and Prevention of Staphylococcus aureus Infections in Orthopaedic Surgery: A Novel Strategy Against Antimicrobial Resistance
by Humera Sarwar, Richard A. Martin, Heather M. Coleman, Aaron Courtenay and Deborah Lowry
Pathogens 2025, 14(8), 760; https://doi.org/10.3390/pathogens14080760 (registering DOI) - 1 Aug 2025
Abstract
This study explores the potential of biodegradable Bioglass 45S5 formulations as a dual-function approach for preventing and treating Staphylococcus aureus infections in orthopaedic surgery while addressing the growing concern of antimicrobial resistance (AMR). The research focuses on the development and characterisation of antibiotic-loaded [...] Read more.
This study explores the potential of biodegradable Bioglass 45S5 formulations as a dual-function approach for preventing and treating Staphylococcus aureus infections in orthopaedic surgery while addressing the growing concern of antimicrobial resistance (AMR). The research focuses on the development and characterisation of antibiotic-loaded BG45S5 formulations, assessing parameters such as drug loading efficiency, release kinetics, antimicrobial efficacy, and dissolution behaviour. Key findings indicate that the F2l-BG45S5-T-T-1.5 and F2l-BG45S5-T-V-1.5 formulations demonstrated controlled antibiotic release for up to seven days, with size distributions of D(10): 7.11 ± 0.806 µm, 4.96 ± 0.007 µm; D(50): 25.34 ± 1.730 µm, 25.20.7 ± 0.425 µm; and D(90): 53.7 ± 7.95 µm, 56.10 ± 0.579 µm, respectively. These formulations facilitated hydroxyapatite formation on their surfaces, indicative of osteogenic potential. The antimicrobial assessments revealed zones of inhibition against methicillin-susceptible Staphylococcus aureus (MSSA, ATCC-6538) measuring 20.3 ± 1.44 mm and 24.6 ± 1.32 mm, while for methicillin-resistant Staphylococcus aureus (MRSA, ATCC-43300), the inhibition zones were 21.6 ± 1.89 mm and 22 ± 0.28 mm, respectively. Time-kill assay results showed complete bacterial eradication within eight hours. Additionally, biocompatibility testing via MTT assay confirmed cell viability of >75%. In conclusion, these findings highlight the promise of antibiotic-loaded BG45S5 as a multifunctional biomaterial capable of both combating bone infections and supporting bone regeneration. These promising results suggest that in vivo studies should be undertaken to expedite these materials into clinical applications. Full article
(This article belongs to the Special Issue Antimicrobial Resistance in the Post-COVID Era: A Silent Pandemic)
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16 pages, 1365 KiB  
Article
Immobilization of Cd Through Biosorption by Bacillus altitudinis C10-4 and Remediation of Cd-Contaminated Soil
by Tianyu Gao, Chenlu Zhang, Xueqiang Hu, Tianqi Wang, Zhitang Lyu and Lei Sun
Microorganisms 2025, 13(8), 1798; https://doi.org/10.3390/microorganisms13081798 - 1 Aug 2025
Abstract
In this study, a highly cadmium (II)-resistant bacterium strain, C10-4, identified as Bacillus altitudinis, was isolated from a sediment sample collected from Baiyangdian Lake, China. The minimum inhibitory concentration (MIC) of Cd(II) for strain C10-4 was 1600 mg/L. Factors such as the [...] Read more.
In this study, a highly cadmium (II)-resistant bacterium strain, C10-4, identified as Bacillus altitudinis, was isolated from a sediment sample collected from Baiyangdian Lake, China. The minimum inhibitory concentration (MIC) of Cd(II) for strain C10-4 was 1600 mg/L. Factors such as the contact time, pH, Cd(II) concentration, and biomass dosage affected the adsorption of Cd(II) by strain C10-4. The adsorption process fit well to the Langmuir adsorption isotherm model and the pseudo-second-order kinetics model, based on the Cd(II) adsorption data obtained from the cells of strain C10-4. This suggests that Cd(II) is adsorbed by strain C10-4 cells via a single-layer homogeneous chemical adsorption process. According to the Langmuir model, the maximum biosorption capacity was 3.31 mg/g for fresh-strain C10-4 biomass. Cd(II) was shown to adhere to the bacterial cell wall through SEM-EDS analysis. FTIR spectroscopy further indicated that the main functional sites for the binding of Cd(II) ions on the cell surface of strain C10-4 were functional groups such as N-H, -OH, -CH-, C=O, C-O, P=O, sulfate, and phosphate. After the inoculation of strain C10-4 into Cd(II)-contaminated soils, there was a significant reduction (p < 0.01) in the exchangeable fraction of Cd and an increase (p < 0.01) in the sum of the reducible, oxidizable, and residual fractions of Cd. The results show that Bacillus altitudinis C10-4 has good potential for use in the remediation of Cd(II)-contaminated soils. Full article
(This article belongs to the Section Environmental Microbiology)
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28 pages, 7617 KiB  
Article
Using Circuit Theory to Identify Important Ecological Corridors for Large Mammals Between Wildlife Refuges
by Büşra Kalleci and Özkan Evcin
Diversity 2025, 17(8), 542; https://doi.org/10.3390/d17080542 (registering DOI) - 1 Aug 2025
Abstract
Habitat fragmentation restricts the movement of large mammals across broad landscapes, leading to isolation of individuals or groups, reduced interaction with other species, and limited access to vital resources in surrounding habitats. In this study, we aimed to determine the wildlife ecological corridors [...] Read more.
Habitat fragmentation restricts the movement of large mammals across broad landscapes, leading to isolation of individuals or groups, reduced interaction with other species, and limited access to vital resources in surrounding habitats. In this study, we aimed to determine the wildlife ecological corridors for five large mammals (Ursus arctos, Cervus elaphus, Capreolus capreolus, Sus scrofa, and Canis lupus) between Kastamonu Ilgaz Mountain Wildlife Refuge and Gavurdağı Wildlife Refuge. In the field studies, we used the transect, indirect observation, and camera-trap methods to collect presence data. Maximum Entropy (MaxEnt) (v. 3.4.1) software was used to create habitat suitability models of the target species, which are based on the presence-only data approach. The results indicated that AUC values varied between 0.808 and 0.835, with water sources, stand type, and slope contributing most significantly to model performance. In order to determine wildlife ecological corridors, resistance surface maps were created using the species distribution models (SDMs), and bottleneck areas were determined. The Circuit Theory approach was used to model the connections between ecological corridors. As a result of this study, we developed connectivity models for five large mammals based on Circuit Theory, identified priority wildlife ecological corridors, and evaluated critical connection points between two protected areas, Ilgaz Mountain Wildlife Refuge and Gavurdağı Wildlife Refuge. These findings highlight the essential role of ecological corridors in sustaining landscape-level connectivity and supporting the long-term conservation of wide-ranging species. Full article
(This article belongs to the Special Issue Habitat Assessment and Conservation Strategies)
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12 pages, 1839 KiB  
Article
A Knowledge–Data Dual-Driven Groundwater Condition Prediction Method for Tunnel Construction
by Yong Huang, Wei Fu and Xiewen Hu
Information 2025, 16(8), 659; https://doi.org/10.3390/info16080659 (registering DOI) - 1 Aug 2025
Abstract
This paper introduces a knowledge–data dual-driven method for predicting groundwater conditions during tunnel construction. Unlike existing methods, our approach effectively integrates trend characteristics of apparent resistivity from detection results with geological distribution characteristics and expert insights. This dual-driven strategy significantly enhances the accuracy [...] Read more.
This paper introduces a knowledge–data dual-driven method for predicting groundwater conditions during tunnel construction. Unlike existing methods, our approach effectively integrates trend characteristics of apparent resistivity from detection results with geological distribution characteristics and expert insights. This dual-driven strategy significantly enhances the accuracy of the prediction model. The intelligent prediction process for tunnel groundwater conditions proceeds in the following steps: First, the apparent resistivity data matrix is obtained from transient electromagnetic detection results and standardized. Second, to improve data quality, trend characteristics are extracted from the apparent resistivity data, and outliers are eliminated. Third, expert insights are systematically integrated to fully utilize prior information on groundwater conditions at the construction face, leading to the establishment of robust predictive models tailored to data from various construction surfaces. Finally, the relevant prediction segment is extracted to complete the groundwater condition forecast. Full article
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