Search Results (837)

Human rhinoviruses (HRVs) are the primary cause of the common cold, a highly contagious upper respiratory tract infection characterized by nasal congestion, sneezing, and sore throat. HRV replication depends on its 3C protease (HRV-3Cpro), a key enzyme that cleaves the viral polyprotein into functional proteins essential for viral maturation. Currently, no FDA-approved inhibitors specifically target HRV-3Cpro. While rupintrivir, a synthetic inhibitor, advanced to clinical trials, it ultimately failed due to limited efficacy. This study investigated the potential of Vitex negundo (or lagundi)—a medicinal plant traditionally used in the Philippines for treating colds and respiratory ailments—as a source of natural HRV-3Cpro inhibitors through in silico molecular docking and pharmacokinetic (ADMET) evaluation. Fifteen phytochemicals were screened, with five compounds exhibiting strong binding affinities exceeding that of the reference inhibitor rupintrivir (−6.1 kcal/mol): agnuside (−6.9 kcal/mol), luteolin 7-O-glucoside (−6.7 kcal/mol), 2′-p-hydroxybenzoyl mussaenosidic acid (−6.5 kcal/mol), 6′-(p-hydroxybenzoyl) mussaenosidic acid (−6.5 kcal/mol), and luteolin (−6.2 kcal/mol). Among these, luteolin emerged as a particularly promising lead compound, forming stable hydrogen bonding and hydrophobic interactions with HRV-3Cpro. Luteolin also demonstrates a favorable ADMET and safety profile, predicted to be non-mutagenic and non-hepatotoxic. These findings position luteolin as a potential plant-based HRV-3Cpro inhibitor, warranting further in vitro and in vivo studies to validate its antiviral efficacy and pharmacokinetic properties. Full article

OAS1 (2′–5′-oligoadenylate synthetase 1) and OAS3 have been identified through a genome-wide association study as major loci associated with COVID-19. The rs10774671A>G variant affects alternative splicing and generates two distinct mRNA and protein isoforms. The A allele produces the shorter p42 isoform, which has been associated with increased susceptibility, greater disease severity, and higher mortality from COVID-19, whereas the G allele produces the longer p46 isoform, which has been associated with a protective effect. In addition, the functional variants OAS1 rs4767027C>T, OAS1 rs1131454A>G, and OAS3 rs10735079A>G have also been associated with susceptibility to and/or severity of COVID-19. Therefore, the aim of this study was to determine whether four variants in the OAS1 and OAS3 genes are associated with susceptibility to COVID-19 and with the clinical signs and symptoms of the disease. We included 305 patients with COVID-19 and 288 healthy controls. We genotyped the OAS1 rs10774671A>G, rs4767027C>T, rs1131454A>G, and OAS3 rs10735079A>G variants using TaqMan^® assays. The association between OAS1 and OAS3 variants and disease susceptibility or severity was assessed using binary logistic regression adjusted for age and sex. The Hardy–Weinberg equilibrium was evaluated using SNPStats, whereas haplotypes and linkage disequilibrium were analyzed with Haploview. Statistical power was calculated using Quanto. Logistic regression analysis adjusted for age and sex revealed an association between the OAS1 rs10774671A risk allele and susceptibility to COVID-19 (G vs. A: OR = 1.9, p = 0.007). In contrast, no associations with COVID-19 susceptibility were observed for the rs4767027C>T, rs1131454A>G, or rs10735079A>G variants. However, the rs1131454A>G and rs10735079A>G variants showed associations with sore throat. Overall, our findings suggest that OAS1 acts as a susceptibility factor for COVID-19 and the rs1131454A>G and rs10735079A>G SNVs are associated with sore throat in the Mexican population. Full article

Ginger supplementation is proposed as a natural ergogenic aid due to its anti-inflammatory and antioxidant properties, but its effects on endurance performance remain unclear. Methods: In this randomized, double-blind, placebo-controlled crossover trial, 30 trained cyclists (27 male, 3 females, aged 36 ± 11 yr) completed three visits: a baseline 75 km time trial, a post-supplementation time trial, and a second post-supplementation trial under the alternate condition. Participants consumed either ginger or placebo for four weeks. Statistics: Performance outcomes (i.e., 75 km time, VO₂, power output, heart rate, and RPE) were analyzed using repeated-measures ANOVA, with repeated-measures ANCOVA to assess dietary and age influences (p < 0.05). Results: Energy and carbohydrate intake were consistent across trials and unrelated to performance. Protein intake per kg body mass predicted performance time in the placebo trial and average VO₂ in the ginger trial; other macronutrients were not associated with outcomes. No significant differences were observed between ginger and placebo conditions for time to completion, VO₂, power output, heart rate, or perceived muscle soreness. Conclusions: Four weeks of ginger supplementation does not improve prolonged cycling performance, recovery, or muscle soreness in trained cyclists when dietary intake is controlled. Future research should explore cellular mechanisms to determine whether ginger supplementation could influence performance or recovery in endurance athletes. Full article

Arcanobacterium haemolyticum is a facultative anaerobic, Gram-positive bacillus that has garnered attention due to its role in human infections, particularly among adolescents and young adults. Traditionally associated with pharyngitis, this organism is increasingly recognized for its involvement in systemic infections, including bacteremia, central nervous system abscesses, and Lemierre’s syndrome. The pathogenicity of A. haemolyticum is attributed to its production of hemolysins and neuraminidase, facilitating tissue invasion and immune evasion. Clinically, infections often present with sore throat, fever, and a characteristic scarlatiniform rash, which can lead to their misdiagnosis as streptococcal pharyngitis. Severe manifestations, though rare, have been documented, particularly in immunocompromised individuals. Diagnosis is challenging due to the organism’s slow growth and potential misidentification as diphtheroids in cultures. Accurate identification necessitates specific culture conditions and biochemical testing. Treatment typically involves beta-lactam antibiotics; however, the emergence of resistance patterns necessitate susceptibility testing to guide therapy. This review aims to consolidate current knowledge on A. haemolyticum, emphasizing its clinical presentations, diagnostic challenges, and management strategies, thereby enhancing recognition and treatment of infections caused by this emerging pathogen. Full article

Background: The root of Schisandra propinqua (Wall.) Baill. var. sinensis Oliv is a traditional ethnomedicine in China; it was widely used to treat abscesses, sores, carbuncles, rheumatism, and so on. The purpose of this study was to elucidate the bacteriostatic mechanism of the ethyl acetate extract from the root of Schisandra propinqua (Wall.) Baill. var. Sinensis Oliv (Xiao Xue Teng) against Staphylococcus aureus ATCC 25923 (S. aureus ATCC 25923). Methods: Bioactive bacteriostatic constituents in Xiao Xue Teng were identified through Hybrid Quadrupole-TOF LC/MS/MS. The minimum inhibitory concentration (MIC) of Xiao Xue Teng against S. aureus ATCC 25923 was determined using the microbroth dilution method. A time–kill curve analysis was used to evaluate the bacteriostatic effects. SDS-PAGE coupled with nano-liquid NanoLC-ESI-MS/MS, real-time PCR, and scanning electron microscopy (SEM) was used to study the bacteriostatic mechanism of Xiao Xue Teng against S. aureus ATCC 25923. Results: The MIC of Xiao Xue Teng against S. aureus ATCC 25923 was determined to be 15.625 µg/mL. The translation initiation factor (IF-2) and elongation factor (EF-Tu) were significantly decreased in S. aureus ATCC 25923 after treatment with Xiao Xue Teng, while the proteins SodA and AhpC were obviously increased. The intracellular levels of total reactive oxygen species (ROS) and hydrogen peroxide (H₂O₂) were significantly increased (p < 0.01) after the treatment with Xiao Xue Teng. Concurrently, the activities of SOD, CAT and GSH-Px were significantly increased (p < 0.01). Moreover, cellular swelling and shrinkage were observed using SEM. Conclusions: The bacteriostatic mechanism of Xiao Xue Teng against S. aureus ATCC 25923 was related to eliciting oxidative stress, inhibiting protein synthesis and enhancing cytoplasmic membrane permeability. Full article

Objective: The present study aimed to verify two key hypotheses: (1) whether the session rating of perceived exertion (sRPE) can serve as a reliable indicator for workload monitoring in cricket and (2) to compare the injury risk sensitivity of sRPE-derived indicators—including the coupled and uncoupled acute:chronic workload ratio (ACWR), exponentially weighted moving average (EWMA), and robust exponential decreasing index (REDI)—across three pre-specified latency periods (no latency, 7-day latency, and 14-day latency), and to identify the optimal indicator and latency period for cricket injury risk assessment. Material: Twenty-four elite female cricket athletes from the Chinese National Women’s Cricket Team were monitored during daily training throughout the Los Angeles Olympic Games preparation period. Methods: Correlation analysis, Kendall’s tau correlation coefficients, and Bland–Altman plots were employed to assess the relationships and consistency between sRPE and various workload indicators. ROC curves were constructed to compare the performance of sRPE-derived indicators for injury risk across the three pre-specified latency conditions. Results: sRPE and its derived indicators exhibited significant correlations with health status indicators (sleep, fatigue, delayed onset muscle soreness (DOMS), stress, mood, and resting heart rate (RHR)) and physiological and biochemical indicators (testosterone, cortisol), with the majority of these correlations reaching the 0.01 significance level (p < 0.01). Kendall’s tau correlation coefficients and Bland–Altman plots revealed that sRPE and ACWR based on EWMA (hereafter referred to as EWMA) had moderate correlations with health status indicators, while ACWR based on REDI (hereafter referred to as REDI) showed a strong correlation with such indicators. sRPE and its derived indicators were strongly correlated with physiological and biochemical indicators (Kendall’s tau > 0.8) with good consistency, as the majority of scattered points fell within the limits of agreement (mean difference ± 1.96 × standard deviation, MD ± 1.96 × SD). Analysis of injury risk sensitivity indicated that the 7-day latency model yielded the highest average area under the curve (AUC = 0.85). Among all indicators, REDI and EWMA achieved the highest AUC values (AUC = 0.665 and 0.667, respectively; p < 0.001). Notably, EWMA exhibited optimal performance in the 7-day latency time series (AUC = 0.859, >0.80), followed by REDI under the 7-day latency condition (AUC = 0.857). Conclusion: EWMA with 7-day latency is a more sensitive indicator for detecting injury risk. Full article

Background/Objectives: Inappropriate antibiotic use in children is a major driver of antimicrobial resistance (AMR), especially in low- and middle-income countries. In Georgia, little is known about parental behaviors related to pediatric antibiotic use. This cross-sectional study assessed knowledge, attitudes, and practices (KAP) related to antibiotic use and AMR among parents of preschoolers in Tbilisi kindergartens, identifying factors associated with inappropriate practices to inform stewardship interventions. Methods: During March–May 2025, we conducted a two-stage cluster-sampled cross-sectional survey of parents of children aged 2–6 years attending public kindergartens in Tbilisi, Georgia. We assessed knowledge, attitudes, and practices concerning antibiotics and AMR. Multivariable Poisson regression was utilized to identify factors associated with inappropriate antibiotic use, defined as any of the following: early discontinuation, use without a prescription, or saving leftovers. Results: In total, 64.3% (95% CI 60.3–68.1%) reported antibiotic use in the past year, with 27.9% of respondents engaging in ≥1 inappropriate practice. Common reasons for use included bronchitis (35.9%) and sore throat (20.0%); Most antibiotics were prescribed by physicians (77.5%) or administered directly in a clinical setting (16.8%); 18.6% were obtained without a prescription. Forty percent believed antibiotics kill viruses. High knowledge (aPR 0.66, 95% CI 0.45–0.97) was independently associated with lower inappropriate practice; other sociodemographic factors were not significantly associated. Conclusions: Inappropriate pediatric antibiotic use in Tbilisi remains widespread and is linked to parental knowledge gaps rather than demographic factors. Interventions should prioritize correcting key misconceptions among caregivers, while addressing prescribing practices within outpatient settings. Strengthening both public and provider awareness may be essential for effective AMR containment in Georgia. Full article

Background/Objectives: Broccoli-derived glucoraphanin (a sulforaphane precursor that activates Nrf2 defenses) may aid repair; however, its short-term effects in humans remain unknown. This study aimed to evaluate whether short-term supplementation with broccoli-derived glucoraphanin improves recovery from exercise-induced muscle damage. We hypothesized that short-term supplementation with broccoli-derived glucoraphanin would attenuate exercise-induced muscle damage and accelerate recovery. Methods: In a randomized, double-blind, placebo-controlled crossover design, fifteen participants consumed either high-glucoraphanin broccoli powder (320 μg) or placebo for two weeks, followed by elbow flexor eccentric exercise. Strength, soreness, creatine kinase (CK), range of motion (ROM), arm girths, and ultrasound-assessed muscle and tendon morphology were measured at baseline, immediately post-exercise, and at 48 and 96 h post-exercise. Results: Significant main effects of time were observed for isometric and isokinetic torque (p < 0.05), CK (p < 0.05), soreness (p < 0.05), and structural swelling markers (p < 0.05), confirming exercise-induced muscle damage. However, there were no significant Time × Supplement interactions for any variable (p > 0.05), indicating that glucoraphanin did not influence recovery dynamics. Conclusions: These findings suggest that short-term high-dose broccoli supplementation reconstituted with hot water does not modulate recovery following eccentric muscle damage under the conditions tested, including the chosen preparation method and experimental context. Full article

Background: Adults on maintenance hemodialysis experience multiple physical and psychological symptoms that can affect confidence in self-management and perception of care received from healthcare providers. Understanding the interplay between symptom burden, self-management self-efficacy, and perceptions about care received is essential to inform patient-centered nephrology nursing. Aim: This cross-sectional study aimed to describe dialysis symptom burden, self-efficacy to manage chronic disease, and satisfaction with nursing care, and to examine associations among these variables in adults undergoing maintenance hemodialysis in Oman. Methods: A cross-sectional study using consecutive sampling was conducted among 232 adults on maintenance hemodialysis at two dialysis units in Muscat, Oman. Data were collected using the Dialysis Symptom Index, the nursing care satisfaction questionnaire, and the self-efficacy scale. Descriptive, correlation, and multivariable linear regression analysis were used to summarize the findings. Results: The mean age was 55.9 years and the most common comorbidities were diabetes (58.2%) and hypertension (74.1%). Symptom burden was substantial, with over half reporting muscle soreness, anxiety, sleep disturbance, dry mouth, pruritus, appetite loss, and dyspnea, although severity was generally mild–moderate (1.1–1.6/4). Satisfaction with nursing care was high (90.2%), while self-efficacy was moderate (mean 30.52/44). Patient satisfaction correlated positively with self-efficacy (r = 0.25, p < 0.001), but not with symptom burden (r = 0.08, p = 0.24); Self-efficacy showed a small positive correlation with dialysis symptom burden (r = 0.14, p = 0.03), suggesting that patients who were more aware of and reported more symptoms also perceived themselves as more actively engaged in managing their illness. In multivariable analysis, higher satisfaction and more favorable laboratory indicators independently predicted higher self-efficacy. Conclusions: Adults on hemodialysis reported high satisfaction with nursing care but continued to experience multiple physical and psychological symptoms and had only moderate self-efficacy to manage their condition. There is a need to integrate structured symptom assessment and targeted, nurse-led self-management support intervention into routine dialysis care to reduce symptom burden and enhance patients’ confidence in managing their illness. Full article

Objective: The oral cavity is the beginning of the digestive tract and the composition of saliva could indicate immune events in the gut and joints. The objective of this research was to evaluate the diagnostic accuracy of salivary interleukin (IL)-17A for temporomandibular joint (TMJ) internal derangements (IDs) in patients with spondyloarthritis (SpA). Methods: SpA disease activity was assessed using the Bath Ankylosing Disease Activity Index (BASDAI), Ankylosing Spondylitis Disease Activity Score (ASDAS) and Disease Activity Index for Psoriatic Arthritis (DAPSA). Salivary cytokines were analyzed using enzyme-linked immunosorbent assay. TMJ conditions were evaluated using The Diagnostic Criteria for Temporomandibular Disorder (DC/TMD) protocol. A symptomatic TMJ-ID group with intracapsular arthralgia (n = 64) and asymptomatic TMJ-ID group without intracapsular arthralgia (n = 50), regardless of joint sounds, were compared with controls (healthy TMJs, n = 86). Results: Women were more prevalent and salivary IL-17A concentration was higher in both ID groups than in controls. Salivary IL-17A levels positively correlated with erythrocyte sedimentation rate, anti-streptolysin-O titer, salivary IL-12/23 p40 and matrix metalloproteinase-3 levels, sore and swollen joint counts, BASDAI, chronic TMJ pain and anxiety. IL-17A demonstrated diagnostic accuracy for currently symptomatic (cutoff, 11 pg/mL) and asymptomatic (cutoff, 11.6 pg/mL) TMJ-ID vs. controls. Patients with IL-17A levels above these cutoffs more frequently exhibited disc displacement with reduction and degenerative TMJ disease, higher self-reported spinal pain and higher SpA activity, as assessed by ASDAS, than patients with IL-17A levels ≤ cutoffs. TMJ-related headache and somatization contributed to greater TMJ pain in those with IL-17A > cutoffs, when compared with dichotomous controls. Conclusions: Salivary IL-17A concentration provides an accurate laboratory marker of SpA activity and enables the diagnosis of both currently symptomatic and asymptomatic TMJ-IDs in patients with SpA. Full article

The dried leaves of Turpinia arguta (Lindl.) Seem, a traditional Chinese medicinal herb, have been used for the treatment of tonsillitis, sore throat, throat arthralgia, and novel coronavirus pneumonia. This plant possesses significant medicinal, economic, and ecological values. Assessing its distribution patterns and its response to global climate change is critical for the conservation and sustainable use of its resources. This study used GIS technology and ENMTools v1.3 to select 247 distribution records of T. arguta and employed the kuenm R package (running on R v4.4.3, package version 2.0.1) to optimize the MaxEnt model parameters. Based on current and future climate data, this study predicted the current and future potential suitable areas of T. arguta in China during the periods of the 2050s (2041–2060), 2070s (2061–2080), and 2090s (2081–2100) under three SSP emission scenarios (SSP126, SSP245, and SSP585). Additionally, it identified the key environmental variables driving its distribution patterns and conducted a quality suitability regionalization analysis using sample chemical content data. The results show that under current climatic conditions, the highly suitable areas for T. arguta are mainly distributed across five provinces: Jiangxi, Guangdong, Guangxi, Fujian, and Hunan. The distribution of T. arguta is primarily influenced by precipitation and temperature. The suitable ranges of key environmental variables are as follows: average temperature in September > 26 °C (optimal range: 28–32 °C), precipitation in April 175–250 mm, precipitation in September 100–160 mm, annual mean temperature 20–30 °C (optimal range > 22.5 °C), and annual precipitation 1500–2000 mm (peak value: 1750 mm). Quality analysis reveals a positive correlation between ligustroflavone content and the mean diurnal temperature range, as well as between rhoifolin content and soil sand content. Compared with current suitable areas, the total suitable areas of T. arguta are projected to contract by varying degrees across all scenarios in the future. This study will provide a robust scientific basis for guiding the sustainable development/utilization of its resources and optimizing artificial cultivation practices. Full article

Background: Antibiotic misuse and overuse remain a critical driver of antimicrobial resistance (AMR), a global health threat associated with increased morbidity, mortality, and healthcare costs. In Greece, where antibiotic consumption and resistance rates are among the highest in Europe, community pharmacists are well-positioned to contribute to antimicrobial stewardship efforts. Objective: This study aimed to assess the knowledge and attitudes of community pharmacists in Achaia, Western Greece, regarding antibiotic use and AMR, in order to identify knowledge gaps and inform future educational interventions. Methods: A cross-sectional survey was conducted between May and July 2023 among 207 pharmacists using a structured, self-administered questionnaire. The survey assessed demographics, knowledge of antibiotic indications, dispensing practices, and awareness of AMR. Statistical analysis included Chi-square tests and multivariate logistic regression. Results: Pharmacists demonstrated high levels of knowledge regarding appropriate antibiotic use in conditions such as sore throat (95%), bronchitis (76%), influenza (77.5%), and diarrhea (95%). However, knowledge was lower for rhinitis (60%) and sinusitis (56%). Almost all pharmacists (99%) were aware of AMR, and 86% perceived it as a significant public health issue in Greece. Logistic regression showed that pharmacists with 5–10 years of experience were significantly less likely to believe that antibiotics are always effective (OR = 0.08, p = 0.042). Conclusion: Pharmacists in Western Greece are generally well-informed about antibiotic use and AMR, yet misconceptions persist, especially for viral infections. Targeted educational interventions, interprofessional collaboration, and stricter enforcement of prescription regulations are needed to strengthen the role of pharmacists in combating AMR at the community level. Full article

Background/Objectives: Transcranial direct current stimulation (tDCS) is increasingly used by athletes, yet sport-performance-enhancement findings are mixed and often small, with outcomes depending on stimulation target, timing, and task demands. Aim: This scoping review mapped and synthesized the soccer-specific trial evidence to identify (i) which tDCS targets and application schedules have been tested in soccer players, (ii) which soccer-relevant outcomes show the most consistent immediate (minutes–hours) or training-mediated benefits, and (iii) where evidence gaps persist. Methods: We conducted a scoping review of clinical trials in footballers, following review best-practice guidance (PRISMA-informed) and a preregistered protocol. Searches (August 2025) spanned PubMed/MEDLINE, ResearchGate, Google Scholar, and Cochrane, using combinations of “football/soccer” and “tDCS/transcranial direct current stimulation,” with inclusion restricted to trials from 2008–2025. Dual independent screening was applied. Of 47 records identified, 21 studies met the criteria. Across these, the total N was 593 (predominantly male adolescents/young adults; wide range of levels). Results: Prefrontal protocols—most commonly left-dominant dorsolateral prefrontal cortex (DLPFC) (+F3/−F4, ~2 mA, ~20 min)—most consistently improved post-match recovery status/well-being (e.g., fatigue, sleep quality, muscle soreness, stress, mood), and when repeated and/or paired with practice, shortened decision times and promoted more efficient visual search. Effects on classic executive tests were inconsistent, and bilateral anodal DLPFC under fatigue increased risk-tolerant choices. Motor-cortex targeting (C3/C4/Cz) rarely changed rapid force–power performance after a single session—e.g., multiple well-controlled trials found no immediate CMJ gains—but when paired with multi-week training (core/lumbar stability, plyometrics, HIIT, sling), it augmented strength, jump height, sprint/agility, aerobic capacity, and task-relevant EMG. Autonomic markers (exercise HR, early HR recovery) showed time-dependent normalization without specific tDCS effects in single-session, randomized designs. In contrast, a season-long applied program that added prefrontal stimulation to standard recovery reported significantly reduced creatine kinase. Across studies, protocols and masking were athlete-friendly and rigorous (~2 mA for ~20 min; robust sham/blinding), with only mild, transient sensations reported and no serious adverse events. Conclusions: In soccer players, tDCS shows a qualified pattern of benefits that follows a specificity model: prefrontal stimulation can support post-match recovery status/well-being and decision efficiency, while M1-centered stimulation is most effective when coupled with structured training to bias neuromuscular adaptation. Effects are generally modest and heterogeneous; practitioners should treat tDCS as an adjunct, not a stand-alone enhancer, and align montage × task × timing while monitoring individual responses. Full article

Background: Adiponectin is an adipokine with insulin-sensitizing, anti-inflammatory, and cytoprotective properties that also plays a key role in metabolic adaptation to exercise. Although its regulation after resistance exercise has been extensively documented, less is known about its short-term modulation and its correlation with muscle damage markers following resistance training. Methods: Nine resistance-trained young men completed two sessions of total-body resistance exercise: (1) high time under tension (TUT) (5-1-2-1 cadence, to failure; ETS1) and (2) moderate TUT (2-1-2-1 cadence, two repetitions in reserve; ETS2). Plasma and saliva samples were collected before exercise and at 15 min, 24 h, and 48 h after exercise to assess total adiponectin by ELISA. Plasma creatine kinase (CK) and a Visual Analog Scale (VAS) were also measured for muscle soreness. Results: Plasma adiponectin significantly decreased from baseline to 48 h post-exercise in both sessions (p < 0.001), with no differences between the TUT conditions. Salivary adiponectin remained unchanged. Although a significant increase in CK and a decrease in adiponectin were observed at the group level, correlation analysis revealed no significant linear relationship between the magnitude of CK elevation and adiponectin reduction. Conclusions: Overall, these findings support the role of adiponectin as a marker of acute metabolic adaptation to resistance exercise. Acute resistance exercise elicited a time-dependent decrease in circulating adiponectin, irrespective of TUT. The temporal pattern of adiponectin decrease coincided with the rise in muscle damage markers, yet the lack of direct correlation suggests distinct regulatory mechanisms, while the lack of salivary changes underscores the complexity of adipokine regulation in vivo and suggests that saliva is not a reliable indicator of changes in circulating adiponectin. Full article

This study investigated the effects of regulated deficit irrigation on quality and postharvest characteristics of ‘Soreli’ kiwifruit (Actinidia chinensis Planch.). Plants were irrigated at 100% (control), 80%, and 60% of the standard water supply. Fruit quality was monitored by assessing weight loss (WL), firmness, soluble solids content (SSC), and color stability. Bioactive compounds, such as polyphenols (POL), flavonoids (FLAV), ascorbic acid (AA), β-carotene (Car), and chlorophyll (Chl) content and antioxidant enzyme activities, including ascorbate peroxidase (APX), superoxide dismutase (SOD), and catalase (CAT), and the 2,2-azinobis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assay were also evaluated. Results indicated that reduced irrigation at 60% of water supply enhanced antioxidant enzyme levels, without negatively affecting fruit quality parameters: greater resistance to firmness loss, higher soluble solids accumulation, and better color stability. In the early stages of cold storage, fruits under the 60% irrigation treatment showed higher POL, FLAV, and ABTS values, with polyphenols exceeding 200 mg GAE 100 g⁻¹ FW and FLAV content ranging from 4.69 to 5.53 mg CE 100 g⁻¹ FW. The 80% irrigation treatment showed a moderate biochemical response without altering quality. Controlled water deficit can enhance antioxidant activity and bioactive compounds, improving fruit quality and the environmental and commercial value of ‘Soreli’ kiwifruit. Full article