Search Results (109)

Tyrannosaurs were giant predatory dinosaurs that occupied the apex of Late Cretaceous food chains. Little is known about the early life and reproductive ecology of tyrannosaurs due to the extreme rarity of hatchling and juvenile fossils. We report bones of hatchlings (<1 yr) for Tyrannosaurus rex and Gorgosaurus libratus, weighing ~2.5 kg and ~2.4 kg, respectively, i.e., <0.1% of adult mass. Clutches were likely large. We conservatively estimate ~20 eggs in a small adult T. rex versus ~30 eggs in the largest T. rex, and clutches of ~15 eggs in G. libratus; larger clutches of 50 or even 100 eggs are not impossible. This suggests an r-selected reproductive strategy. Synchrotron scans reveal Haversian bone remodeling, suggesting that tyrannosaurs moved soon after hatching. Hatchling tyrannosaurs’ small size and precociality suggest limited parental care; teeth of hatchlings show wear suggesting that they fed on relatively large vertebrates. Tyrannosaurs had proportionately larger offspring than most reptiles, but relatively smaller hatchlings than Eumaniraptora and birds, suggesting retention of a primitive reproductive strategy intermediate between that of basal diapsids and birds. Multiple dinosaur lineages evolved large eggs independently, suggesting an evolutionary trend towards increased parental investment. Full article

Chromosomal rearrangements that lead to the formation of oncogenic gene fusions, such as EML4-ALK, are thought to arise from incorrect repair of double-strand breaks in DNA. However, the mechanisms and factors driving rearrangement formation remain poorly understood, and analysis of these processes is limited by detection methods that are labor-intensive, low-throughput, and not readily quantitative at single-cell resolution. Here, we developed a genetically encoded ALK reporter based on A549 lung adenocarcinoma cells, created by inserting an ALK-P2A-mCherry cassette into the endogenous ALK locus, so that induced EML4-ALK fusion activated mCherry fluorescence. Reporter activation yielded a readily quantifiable mCherry-positive subpopulation that could be measured and enriched by flow cytometry and correlated with EML4-ALK levels. Using this platform, we combined CRISPR-mediated rearrangement induction with knockdown of DNA repair factors using RNA interference. Of the factors involved in base excision repair, homologous recombination-related pathways and canonical non-homologous end joining, knockdown of the APEX1 gene encoding apurinic endonuclease 1 (APE1) selectively increased EML4-ALK levels both in the reporter cell line and in parental A549 cells. Together, this work provides a sensitive, single-cell A549-based ALK reporter platform and a framework for future studies aimed at identifying cellular and environmental factors that modulate oncogenic EML4-ALK rearrangement formation. Full article

Introduction: Longlines are traditional fishing gear and are widely used in marine systems for both quantitative and qualitative sampling of large predators like tuna, swordfish, sailfish, marlin or sharks. This highly selective method has been applied for the sampling of European catfish (Silurus glanis), the largest freshwater fish in Europe. This apex predator is a highly valuable fish species in its native localities of Central and Eastern Europe (commercial fishing, anglers’ trophy fishing, biomanipulation purposes) but a dangerous alien invasive species in South and Western Europe. Objective: The efficiency and selectivity of longlines for European catfish sampling were compared with more traditional fishing methods like gillnets and electrofishing. Methodology: European catfish were sampled in native areas (Římov, Žlutice, Vrchlice, Hubenov, Lipno, Želivka reservoirs; Czech Republic) and areas of invasion (Lakes Campagna, Avigliana Grande, Maggiore; Italy; Belver, Meimoa, Cedillo reservoirs; Portugal; Iznajar, Mequinenza reservoirs; Spain) following the protocols of best catfish (Silurus glanis) capture methodologies in small and large lakes and reservoirs established for the current needs of the European Commission within the LIFE PREDATOR project. Results: The longline efficiency expressed as the proportion of individual hooks catching catfish (live baits exposed overnight) was from low to medium (5–25%; Czech reservoirs, Italian lakes, Meimoa reservoir) to very high (up to over 50%; Portuguese and Spanish reservoirs). In many water bodies, specifically in areas of invasion, catfish represented 100% of the longline catch (Campagna, Maggiore, Cedillo, Iznajar, Mequinenza). In the rest of the water bodies, the by-catch ranged from 2 to 20% with the highest values occurring in its native localities (due to the presence of large individuals of Northern pike, Esox lucius, another predatory fish in these systems). In areas of invasion, the inconsiderable by-catch was composed of other non-native predatory fish species like pikeperch (Sander lucioperca), European perch (Perca fluviatilis) or channel catfish (Ictalurus punctatus). Both the efficiency and selectivity of longlines were higher compared to gillnets and electrofishing, and longlines also caught larger catfish individuals. Conclusions: Longlines represent an efficient and extremely selective method for European catfish sampling, reduction and even eradication, especially in areas of invasion. Their usage, however, requires specific equipment, skills and also baits. Full article

Background and Objectives: The labyrinthine artery (LA) is a small but surgically important vessel encountered during procedures involving the cerebellopontine angle (CPA). Variations in its number, origin, and relationship to the facial–vestibulocochlear nerve complex may increase the risk of vascular injury during CPA surgery. The aim of this cadaveric microdissection study was to evaluate the number, origin, and course of the LA within the CPA and to characterize its relationship to the anterior inferior cerebellar artery (AICA) and the facial–vestibulocochlear nerve complex. Materials and Methods: Microsurgical dissections were performed bilaterally in 45 formalin-fixed adult cadavers (90 CPAs) using an operating microscope and vascular injection. The number, origin, and course of the LA were analyzed together with its relationship to the AICA and the facial–vestibulocochlear nerve complex. Results: The LA was identified in all specimens. A single LA was observed in 57.8% of CPAs, whereas multiple LAs were identified in 42.2%. The artery most commonly originated from the AICA (65.6%), followed by a common trunk shared with the AICA (21.1%) and direct origin from the basilar artery (13.3%). In specimens with bifurcated AICAs forming double loops, multiple LAs frequently arose from both loops. Considerable variability was observed in the course of the LA relative to the facial–vestibulocochlear nerve complex, including superior, inferior, and interposed courses. Bilateral asymmetry was identified in 15.6% of cadavers. Conclusions: The LA demonstrates substantial anatomical variability within the CPA regarding its number, origin, and neurovascular relationships. Although the artery most commonly arises from the apex or medial aspect of the AICA loop, its subsequent course may vary considerably. Careful microsurgical inspection remains essential during CPA surgery, as the configuration of the AICA alone is insufficient to reliably predict the distal trajectory of the LA. Full article

Constructing inverted wedge-shaped hydrophilic channels with a small apex angle on surfaces with wettability patterns is an effective strategy to promote efficient and complete droplet detachment, which is crucial for applications such as condensation heat transfer and self-cleaning. However, a comprehensive understanding of how wedge geometry parameters affect droplet dynamics has not been established. In this study, we systematically investigate the dynamics of droplet formation and detachment within inverted wedge-shaped superhydrophilic channels fabricated by laser etching on hydrophobic or superhydrophobic substrates. Four distinct droplet detachment mechanisms are revealed. Our results indicate that, within the experimental parameters tested, a slender channel geometry—featuring a narrow upper base, a minimized lower base, and sufficient height—combined with a superhydrophobic substrate, promotes high-position droplet formation, extends the droplet sliding distance, and significantly reduces resistance. This synergy leads to the most efficient detachment mechanism: inertia-driven direct shedding. For the tested configurations, the C1.2/0/40 channel achieved the highest recorded detachment frequency of 318 min⁻¹ at a flow rate of 0.5 mL/min. Furthermore, droplet rebound at the channel tip is observed in some configurations, where two to three droplets must form sequentially and coalesce to trigger a single detachment event. This work provides actionable geometric design strategies for engineering surfaces capable of directional and highly efficient droplet detachment. Full article

Pycnospatha is a small and poorly known genus of Araceae distributed in Indochina and currently comprising only two accepted species. During botanical surveys in Si Sa Ket Province, eastern Thailand, an unusual population of Pycnospatha was discovered in a dry dipterocarp forest and found to differ from both P. arietina and P. palmata. Here, we describe this plant as a new species, Pycnospatha phanomdongrakensis. The new species is distinguished by a combination of characters, including a slender habit, shorter petiole and peduncle, a medium-sized spathe, a short and dense spadix, a distinctly curved style directed toward the apex of the spadix, a geophilous and ovoid infructescence, obovate berries, and asymmetrically ovoid seeds. The new taxon is currently known only from a single population in the Phanom Dong Rak mountain range. A preliminary conservation assessment is provided, and the species is treated as Critically Endangered (CR) following IUCN guidelines. An identification key to all species of Pycnospatha is also presented. The discovery of this new species highlights the continuing importance of field-based taxonomy in revealing overlooked aroid diversity in the seasonally dry forests of eastern Thailand. Full article

To address the severe multiple-seed pickup problem during the seed-filling process of an air-suction seed metering device for small-seed crops with multiple seeds per hill, a combined seed-cleaning mechanism consisting of an upper seven-tooth seed-cleaning device and a lower seed-cleaning blade was developed based on an analysis of the causes of multiple pickup. Mathematical models of seed motion and force were established to describe the interaction between the seven-tooth seed-cleaning device and the seed population during the cleaning process. The installation position and adjustment mechanism of the device on the seed chamber housing were determined, and its tooth-profile parameters and major operating positions were theoretically analyzed. Accordingly, the design method and calculation models for the key parameters of the seven-tooth seed-cleaning device were established. A quadratic regression orthogonal rotational combination experiment was conducted using three factors affecting cleaning performance: the distance between the apex of the first tooth and the corresponding suction hole, the operating speed of the seed metering device, and the negative pressure. Regression equations were established and response surface analysis was performed. With the seed-cleaning qualification rate as the optimization objective, the optimal parameter combinations were obtained as follows: for millet, 3.36 mm, 3.59 km/h, and 1.43 kPa; for broomcorn millet, 3.49 mm, 4.22 km/h, and 2.11 kPa; and for rapeseed, 3.15 mm, 3.73 km/h, and 1.52 kPa. To reduce the influence of random error, 200 repeated bench tests were conducted for each seed type under its corresponding optimal parameter combination at operating speeds of 2.0–5.0 km/h. The seed-cleaning qualification rates for millet, broomcorn millet, and rapeseed were all above 90%, meeting the design requirements of the seed-cleaning mechanism. This study provides a theoretical basis and technical reference for seed-cleaning mechanisms for air-suction precision seed metering devices for small-seed crops with multiple seeds per hill. Full article

The investigation aimed to determine if live feedback of team- and player-specific global positioning system (GPS) running performance data between bouts of hurling small-sided games (SSGs) altered the physical and physiological responses during subsequent bouts of SSGs during a 6-week hurling pre-season period. Twenty-four (n = 24) hurling players (age 25.5 ± 3.2 years; height 177.9 ± 3.2 cm; body mass 83.5 ± 4.5 kg) received either feedback or no feedback during hurling-specific SSGs across a 6-week pre-season period. Teams were assigned to two specific groups, a) GPS live feedback or b) no GPS live feedback (control) for each session, with feedback provided during the SSG rest interval. Running performance (10-Hz, STATSports, Apex, Northern Ireland), heart rate (Polar T31 coded, Polar Electro, Finland), and rating of perceived exertion (RPE) were measured. Data was analyzed using linear mixed-effect models with the effect size (Cohen’s d) used to determine the size of the effect between feedback and non-feedback conditions. Trivial-o-small differences at all time points were observed in heart rate and RPE measures during SSGs, respectively. Trivial-to-moderate effects were observed between feedback and non-feedback conditions for total distance (p = 0.04; ES = 0.25; small) high-speed running (p = 0.043; ES = 0.59; moderate), maximal speed (p = 0.345; ES = 0.11; trivial) and accelerations (p = 0.03; ES = 0.55; moderate). The current data suggests that coaches and applied practitioners may use live GPS feedback to alter the running and physiological performance within hurling-specific SSGs during a pre-season period. Full article

Accurate spatial information on individual tree locations is essential for precision forestry, the integration of field and remote sensing data, and tree-level forest analyses. This study compared the positional accuracy and tree identification performance of four tree-mapping approaches: legacy paper maps, a pseudolite-based field positioning system (TerraHärp), drone-based laser scanning, and mobile laser scanning (MLS). The analysis was conducted in five long-term experimental forest sites in Lithuania, comprising pine- and spruce-dominated stands with varying stand densities. Tree locations derived from legacy maps and the TerraHärp system were compared to assess systematic and random positional discrepancies. TerraHärp-derived tree positions were subsequently used as a reference to evaluate the laser scanning-based methods. Positional accuracy was assessed using Hotelling’s T² test, root-mean-square error, and the National Standard for Spatial Data Accuracy (NSSDA), while spatial autocorrelation of deviations was examined using Moran’s I. The results indicated that discrepancies between TerraHärp and legacy maps were dominated by systematic horizontal shifts in the historical maps, whereas random positional variability was relatively small and consistent across stand types. Drone-based laser scanning showed a strong dependence of tree identification accuracy on stand density and mean tree diameter. Overall, CHM-based segmentation yielded more accurate tree identification than 3D point cloud segmentation, with mean F1-scores of 0.78 and 0.72, respectively. Positional accuracy varied by method, with the largest errors from CHM apexes and highest 3D point cloud points (mean NSSDA ≈ 1.8–2.0 m), improved accuracy using the lowest 3D cluster points (1.45–1.72 m), and the highest accuracy achieved using mobile laser scanning (mean NSSDA 0.76–0.90 m; >95% of trees within 1 m). These results demonstrate that pseudolite-based field mapping provides a reliable reference for high-precision tree location and for integrating field and laser scanning data in managed conifer stands. Full article

Background/Objectives: Adolescent idiopathic scoliosis (AIS) is a three-dimensional spinal deformity commonly managed with conservative strategies, including bracing and physiotherapeutic scoliosis-specific exercises (PSSEs). The Canali Postural Method^® (CPM) is an individualized kinesiological approach aimed at postural reprogramming, while the Canali Orthopedic Brace is an intermittent, non-rigid device intended to facilitate active postural control rather than continuous passive correction. Case Presentation: We retrospectively report two adolescent females with thoracolumbar rotoscoliosis (Risser grade 4). Case 1 (15 years) presented with a left-convex thoracolumbar curve (apex T12–L1) with a Cobb angle of 19.4° and a derotation angle ratio (DAR) of 1.9. Case 2 (16 years) presented with a right-convex thoracolumbar curve (apex T10) with a Cobb angle of 41.14° and a DAR of 3.7. Both patients underwent supervised CPM-based exercise sessions combined with intermittent use of the Canali Orthopedic Brace. Discussion and Conclusions: Follow-up radiographs showed a marked reduction in curve magnitude and rotational parameters: in Case 1, the Cobb angle decreased from 19.4° to 4.1° and DAR from 1.9 to 0.4; in Case 2, the Cobb angle decreased from 41.14° to 15.17° and DAR from 3.7 to 1.36. Pelvic asymmetry was also reduced, and no worsening of sagittal alignment was observed. Given the retrospective design, the small sample size, heterogeneity in intervention duration, and the lack of clinical outcomes and formal measurement reliability testing, these findings should be interpreted with caution and warrant confirmation in prospective controlled studies. Full article

Dicer is crucial for the generation of microRNAs (miRNAs), which are essential for regulating gene expression and keeping neuronal health. Dicer’s conditional deletion cuts all spiral ganglion neurons but spares a small fraction of vestibular ganglion neurons, innervating the utricle and part of the saccule. Hair cells develop in the utricle, saccule, posterior crista, and the cochlea in Pax2^Cre; Dicer^f/f. Cochlear hair cells develop at the base and expand the OHC and IHC in the middle, or split into a base/middle and the apex. In contrast, Foxg1^Cre; Dicer^f/f cuts all canal cristae and cochlea hair cells, leaving a reduced utricle and an exceedingly small saccule. Likewise, Foxg1^Cre; Gata3^f/f shows no cochlear hair cells and is absent in the horizontal and reduced in the posterior crista. In contrast, the utricle, saccule, and anterior crista are nearly normal, underscoring the intricate regulatory networks involved in hair cell and neuronal development. The central projections have been described as the topology of various null deletions. Still, without spiral ganglion neurons, fibers from Dicer null mice navigate to the cochlear nuclei and expand into the vestibular nuclei to innervate the caudal brainstem. Beyond a ramification around the CN, no fibers expand to reach the cerebellum, likely due to Pax2 and Foxg1 that cut these neurons. Genetic alterations, such as Dicer deletion, can lead to hearing loss and impairments in auditory signal processing, illustrating the critical role of microRNAs in the development and function of auditory and vestibular neurons. Further studies on this topic could help in understanding potential therapeutic targets for hearing loss associated with neuronal degradation of miRNA. Full article

The current study investigated the relationship between match-play running demands and years of elite hurling experience across the 2021–2023 seasons. Sixty-eight (mean ± SD; Age: 25.5 ± 3.6 years, Mass: 87.5 ± 5.0 kg, Height: 184.2 ± 4.9 cm, Years Elite Experience: 5.3 ± 3.5 years) male elite intercounty hurlers participated. Each participant wore a global positioning system (GPS) unit sampling at 10 Hz (STATSports, Apex, Northern Ireland), and if they played ≥70 min, they were included in the analyses. Distance metrics analysed in metres were total distance (TD), high-speed running (HSR) (≥19.8 km·h⁻¹), sprint distance (≥25.2 km·h⁻¹), and high metabolic load distance (HMLD) (≥25.5 W·kg⁻¹). Participants were split into observational groups based on their years of elite experience: Emerging: 1–3 years, Established: 4–6 years, Seasoned: 7+ years. Emerging players covered less HSR (p = 0.039, ES = 0.25, small) and sprint distance (p = 0.019, ES = 0.28, small) compared to Established players. Seasoned players covered fewer TD and HMLD compared to Emerging players (TD: p < 0.001, ES = 0.31, small; HMLD: p < 0.001, ES = 0.34, small) and Established players (TD: p < 0.001, ES = 0.51, small; HMLD: p < 0.001, ES = 0.32, small). The results identified differences in match-play running demands based on years of elite experience. These findings may guide experience-specific conditioning strategies. Full article

The wolf (Canis lupus) is an apex predator with high mobility and trophic plasticity, serving as a valuable indicator of helminth transmission at the wildlife–livestock interface. Given the ecological overlap between wolves and both wild and domestic ungulates in Romania, we aimed to identify and molecularly characterize cestodes from wolves’ small intestines. Between November 2022 and June 2025, small intestines from nine wolves were collected across four Romanian counties, frozen, and examined using classical parasitology (macroscopic and microscopic) and molecular methods (PCR amplification and Sanger sequencing of mitochondrial cox1, nad1, and 12S rRNA fragments). Taeniids were detected in three (33.33%) out of nine tested individuals. Genetic analyses confirmed the presence of Taenia krabbei and Taenia hydatigena—species not previously reported in wolves from Romania. This study provides the first molecular evidence of T. krabbei and T. hydatigena in wolves from Romania, and likely Eastern Europe, indicating active transmission and underscoring the need for broader surveillance of hosts to clarify their ecology and regional dynamics within a One Health context. Full article

The operation of microgravity research missions, such as sounding rockets, CubeSats, and small landers, typically relies on proprietary mission control infrastructures, which limit reproducibility, portability, and interdisciplinary use. In this work, we present an open-source blueprint for a distributed ground-segment architecture designed to support telemetry, telecommand, and mission operations across institutional and geographic boundaries. The system integrates containerized services, broker bridging for publish–subscribe communication, CCSDS-compliant telemetry and telecommand handling, and secure virtual private networks with two-factor authentication. A modular mission control system based on Yamcs was extended with custom plug-ins for CRC verification, packet reassembly, and command sequencing. The platform was validated during the MAPHEUS-10 sounding rocket mission, where it enabled uninterrupted remote commanding between Sweden and Germany and achieved end-to-end command–response latencies of ~550 ms under flight conditions. To the best of our knowledge, this represents the first open-source ground-segment framework deployed in a space mission. By combining elements from computer science, aerospace engineering, and systems engineering, this work demonstrates how interdisciplinary integration enables resilient, reproducible, and portable mission operations. The blueprint offers a practical foundation for future interdisciplinary research missions, extending beyond sounding rockets to CubeSats, ISS experiments, and planetary landers. This study is part two of a three-part series describing the apex Mk.2/Mk.3 experiments, open-source ground segment, and service module simulator. Full article

After the successful flight of the first Advanced Processors, Encryption, and Security Experiment (apex) Commercial Off-the-Shelf (COTS) On-Board Computer (OBC) during the Propulsion Technologies and Components of Launcher Stages (ATEK)/Material Physics Experiments Under Microgravity (MAPHEUS)-8 sounding rocket campaign, a second generation of COTS OBCs were built, leveraging the knowledge gained. This new concept and improvements are provided. The Mk.2 Science Camera Platform (SCP) has an instrumented high-definition science camera to research the behavior of small organisms such as Trichoplax adhaerens under challenging gravity conditions, while the Mk.3 Student Experiment Sensorboard (SES) represents an Arduino-like board that directly interfaces with the MAPHEUS Service Module and allows for rapid development of new sensor solutions on sounding rocket systems. Both experiments were flown successfully on MAPHEUS-10, including a biological system as a proof of concept, and paved the way for an even more capable third generation of apex OBCs. This study is part one of a three-part series describing the apex Mk.2/Mk.3 experiments, open-source ground segment, and service module simulator. Full article