Search Results (44)

Massachusetts Bay in the northeastern United States is highly vulnerable to ocean acidification (OA) due to reduced buffering capacity from significant freshwater inputs. We hypothesize that acidification varies across temporal and spatial scales, with short-term variability driven by seasonal biological respiration, precipitation–evaporation balance, and river discharge, and long-term changes linked to global warming and river flux shifts. These patterns arise from complex nonlinear interactions between physical and biogeochemical processes. To investigate OA variability, we applied the Northeast Biogeochemistry and Ecosystem Model (NeBEM), a fully coupled three-dimensional physical–biogeochemical system, to Massachusetts Bay and Boston Harbor. Numerical simulation was performed for 2016. Assimilating satellite-derived sea surface temperature and sea surface height improved NeBEM’s ability to reproduce observed seasonal and spatial variability in stratification, mixing, and circulation. The model accurately simulated seasonal changes in nutrients, chlorophyll-a, dissolved oxygen, and pH. The model results suggest that nearshore areas were consistently more susceptible to OA, especially during winter and spring. Mechanistic analysis revealed contrasting processes between shallow inner and deeper outer bay waters. In the inner bay, partial pressure of pCO₂ (pCO₂) and aragonite saturation (Ω_a) were influenced by sea temperature, dissolved inorganic carbon (DIC), and total alkalinity (TA). TA variability was driven by nitrification and denitrification, while DIC was shaped by advection and net community production (NCP). In the outer bay, pCO₂ was controlled by temperature and DIC, and Ω_a was primarily determined by DIC variability. TA changes were linked to NCP and nitrification–denitrification, with DIC also influenced by air–sea gas exchange. Full article

The Portuguese coast forms a key biogeographic transition zone where co-occurring kelp species show limited vertical overlap. This study aimed to understand whether temperature and light responses help explain the vertical niche differentiation of Laminaria ochroleuca, Saccorhiza polyschides, and Phyllariopsis brevipes. Results revealed that P. brevipes, despite occupying the southernmost range, showed a low thermal tolerance: 27 °C significantly increased respiration rates, indicating metabolic stress, and exposition at 30 °C caused physiological stress. In contrast, L. ochroleuca and S. polyschides exhibited a greater thermal resilience but displayed high light requirements, with evident stress at 30 °C. These results suggest that light availability may play a key role in shaping vertical zonation in a climate warming scenario, with species adapted to low light occupying deeper subtidal zones. S. polyschides, a high light-requiring species, dominates the shallow subtidal region, while L. ochroleuca, also high light-requiring and temperature-tolerant, is abundant in both intertidal pools and shallow subtidal habitats. These findings raise new hypotheses regarding future distribution patterns under climate change: while L. ochroleuca may continue expanding polewards and potentially replace other Laminaria spp. at shallow depths, low-light-adapted, cold-water species may retain a competitive advantage in deeper zones. Full article

In this study, we hypothesized and tested that physical parameters (flow, transport, and water depth) have a significantly greater influence on phosphorus (P) retention in wetlands than biogeochemical factors. Specifically, we evaluated the null hypothesis (H₀), that no significant difference exists between the influence of physical and biogeochemical parameters on phosphorus retention, against the alternative hypothesis (H₁), that physical parameters are more influential. We investigated two large wetlands (stormwater treatment areas, STAs) in south Florida: STA34C2A, which is dominated by emergent aquatic vegetation (EAV), and STA2C3, which is dominated by submerged aquatic vegetation (SAV). Building on Part 1, which mapped spatial flow resistance (K) as a vegetation-type-independent proxy for hydraulic resistance, this study (Part 2) applied a novel high-frequency (hourly) data approach with time-lagged regression modeling to estimate total phosphorus (TP) outflow concentrations. The key variables included inflow TP concentration, vegetation volume, water depth, nominal hydraulic residence time (HRT), hydraulic loading rate (HLR), phosphorus loading rate (PLR), and time lag (“P-spiral”). Multi-linear regression models for each STA identified inflow TP and water depth, a controllable physical parameter, as the most significant predictors of TP outflow, while the hour of day (a temporal proxy) contributed the least. Optimal model performance occurred with lag times of 8 and 9 days, producing R² values of 0.5788 (STA34C2A) and 0.5354 (STA2C3). In STA34C2A, high TP retention was linked to shallow water depth, dense EAV, and low K values, indicating high hydraulic resistance and reduced short circuiting. In contrast, lower TP retention in STA2C3 was associated with longer flow paths, sparse SAV, and high K values, suggesting less hydraulic control despite similar nominal HRTs. These results provide empirical support for rejecting the null hypothesis (H₀) in favor of the alternative (H₁): physical parameters, especially water depth, hydraulic resistance, and inflow dynamics, consistently exert a stronger influence on P removal than biogeochemical factors such as PLR. The findings highlight the importance of optimizing flow and depth controls in wetland design and management to enhance phosphorus removal efficiency in large, constructed wetland systems. Full article

Fragipans are dense subsurface soil layers that severely restrict root penetration and water movement. The presence of shallow fragipan horizons limits row crop production. We hypothesized that the roots of cover crop might improve soil physiochemical properties and biological activity, facilitating drainage and increasing effective soil depth for greater long-term soil water storage. To evaluate annual ryegrass as one component of a cover crop (CC) mix for promoting the characteristics and distribution of soil water, on-farm studies were conducted at Marion and Springerton in southern Illinois, USA. Soil samples were collected at 15 cm increments to 60 cm (Marion) and 90 cm (Springerton) depths during the fall of 2022. Both sites had low total soil carbon and nitrogen contents and acidic soil pH (≤6.4). A soil water retention curve was fitted using the van Genuchten equation. At Springerton, the CC treatment increased saturated (thetaS) and residual (thetaR) soil water contents above those of the no cover crop (NCC) at the 60–75 cm and 75–90 cm depths. Changes in volumetric soil water content were measured using a multi-depth soil water sensor for the Springerton site during late July to early August of the soybean growing phase of 2022; NCC had higher soil water than CC within the 0–15 cm depth, but CC had higher soil water than NCC at the 30–45 cm depth. These findings indicate that cover crop mix has the potential to improve soil water movement for soils with restrictive subsoil horizon, possibly through reducing the soil hydraulic gradient between the surface and restrictive subsurface soil layers. Full article

Many municipalities around the world place their production wells in shallow alluvial aquifers that are adjacent to streams. Pumping these wells then induces the infiltration of surface water into the aquifer, allowing the greater extraction of water without significantly depleting the aquifer. However, induced infiltration poses a risk of introducing contamination from surface water into groundwater systems. The goal of this study was to quantify the amount of induced infiltration due to municipal pumping at the Four Mile Creek well field in Oxford, Ohio, using stable isotopes of water oxygen (δ¹⁸O) and deuterium (δ²H). In areas of municipal pumping, we sampled water from the production wells, Four Mile Creek, and from monitoring wells that we hypothesized to be both influenced and not influenced by induced infiltration. Samples were collected over 10 months in 2012 and over 12 months in 2021. In 2012, surface water δ¹⁸O values ranged from −3.89 to −8.04‰, and δ²H ranged from −26.55 to −55.65‰ at sampling sites. PW1 δ¹⁸O values ranged from −4.71 to −7.39‰ with a mean of −6.61 and −32.01 to −47.86‰ with a mean of −42.74‰ for δ²H. PW2 δ¹⁸O values ranged from −5.74 to −7.34‰, with a mean of −6.45‰, and δ²H ranged from −36.29 to −47.82‰ with a mean of −42.43‰. PW3 had lower values of both δ¹⁸O and δ²H, ranging from −6.36 to −8.02‰ and −47.7 to −40.35‰, and with means of −7.08 and −45.11, respectively. In 2021/2022, surface water δ¹⁸O values ranged from −5.32 to −7.93‰, and the δ²H ranged from −36.14 to −50.56‰. PW1 δ¹⁸O values ranged from −6.15 to −7.54‰ with a mean of −7.13‰, and δ²H ranged from −43.52 to −49.01‰ with a mean of −45.99‰. PW2 δ¹⁸O values ranged from −5.72 to −7.34‰, with a mean of −6.70‰, and δ²H ranged from −36.69 to −46.14‰, with a mean of −43.61‰. Using the time averaged values of δ¹⁸O of groundwater, production wells and surface water, the percentages of surface water resulting from induced infiltration in 2012 were 57%, 59% and 15% at the three wells, respectively, while in 2021, PW1 had 35% and PW2 91%. The amount of induced infiltration was apparently related to the pumping rates of the production wells, the length of time of pumping and the distance between Four Mile Creek and production wells. Our results indicate that stable isotopes of water provide a reliable method of quantifying groundwater/surface water interaction in alluvial aquifers. Full article

Due to shallow root systems, potato is a particularly drought-sensitive crop. To counteract these limitations, the application of plant growth-promoting microorganisms (PGPMs) is discussed as a strategy to improve nutrient acquisition and biotic and abiotic stress resilience. However, initial root colonization by PGPMs, in particular, can be affected by stress factors that negatively impact root growth and activity or the survival of PGPMs in the rhizosphere. In this study, perspectives for the use of commercial silicate-based soil conditioners (SCs) supposed to improve soil water retention were investigated. The SC products were based on combinations with lignocellulose polysaccharides (Sanoplant^® = SP) or polyacrylate (Geohumus^® = GH). It was hypothesized that SC applications would support beneficial plant–inoculant interactions (arbuscular mycorrhiza, AM: Rhizophagus irregularis MUCL41833, and Pseudomonas brassicacearum 3Re2-7) on a silty loam soil–sand mixture under water-deficit conditions (6–12 weeks at 15–20% substrate water-holding capacity, WHC). Although no significant SC effects on WHC and total plant biomass were detectable, the SC-inoculant combinations increased the proportion of leaf biomass not affected by drought stress symptoms (chlorosis, necrosis) by 66% (SP) and 91% (GH). Accordingly, osmotic adjustment (proline, glycine betaine accumulation) and ROS detoxification (ascorbate peroxidase, total antioxidants) were increased. This was associated with elevated levels of phytohormones involved in stress adaptations (abscisic, jasmonic, salicylic acids, IAA) and reduced ROS (H₂O₂) accumulation in the leaf tissue. In contrast to GH, the SP treatments additionally stimulated AM root colonization. Finally, the SP-inoculant combination significantly increased tuber biomass (82%) under well-watered conditions, and a similar trend was observed under drought stress, reaching 81% of the well-watered control. The P status was sufficient for all treatments, and no treatment differences were observed for stress-protective nutrients, such as Zn, Mn, or Si. By contrast, GH treatments had negative effects on tuber biomass, associated with excess accumulation of Mn and Fe in the leaf tissue close to toxicity levels. The findings suggest that inoculation with the PGPMs in combination with SC products (SP) can promote physiological stress adaptations and AM colonization to improve potato tuber yield, independent of effects on soil water retention. However, this does not apply to SC products in general. Full article

Ongoing investments in irrigation technologies highlight the need to accurately estimate the longevity and magnitude of water savings at the watershed level to avoid the paradox of irrigation efficiency. This paradox arises when irrigation pumping exceeds crop water demand, leading to excess water that is not recovered by the watershed. Comprehensive water accounting from farm to watershed scales is challenging due to spatial variability and inadequate socio-hydrological data. We hypothesize that water savings are short term, as prior studies show rapid recharge responses to surface changes. Precise estimation of these time scales and water savings can aid water managers making decisions. In this study, we examined water savings at three 65-hectare sites in Nebraska with diverse soil textures, management practices, and groundwater depths. Surface geophysics effectively identified in-field variability in soil water content and water flux. A one-dimensional model showed an average 80% agreement with chloride mass balance estimates of deep drainage. Our findings indicate that groundwater response times are short and water savings are modest (1–3 years; 50–900 mm over 10 years) following a 120 mm/year reduction in pumping. However, sandy soils with shallow groundwater show minimal potential for water savings, suggesting limited effectiveness of irrigation efficiency programs in such regions. Full article

The recovery of submerged macrophytes is crucial for lake restoration. However, Myriophyllum spicatum usually shows an overgrowth and inhibits the growth of Vallisneria denseserrulata via light shading in many restored shallow lakes after the plant transplantation. So far, harvesting M. spicatum is the primary method to alleviate these shading effects in post-restoration lakes. Nevertheless, the effects of harvesting on the growth of V. denseserrulata and water quality are poorly elaborated. In this study, we conducted a mesocosm experiment, including both monoculture and polyculture groups, to investigate the response of V. denseserrulata growth, light climate, and nutrient concentrations in the water with M. spicatum harvesting. Moreover, the growth and morphology of M. spicatum were also examined. We hypothesized that M. spicatum harvesting would enhance the growth of V. denseserrulata and improve both the light climate and water quality. Our results showed that harvesting M. spicatum in the polyculture mesocosms substantially enhanced the relative growth rate (RGR) of V. denseserrulata compared to the non-harvesting controls. Moreover, harvesting M. spicatum reduced the light attenuation coefficient at 30 cm depth; however, the concentrations of chlorophyll-a, total nitrogen, and total phosphorus did not change significantly. As for M. spicatum, harvesting inhibited the growth of main stem and root but did not significantly affect the cumulative weight and RGR of M. spicatum. In contrast, the presence of V. denseserrulata decreased cumulative weight and RGR while promoting the root parameters of M. spicatum. Our findings imply that harvesting overgrowth nuisance submerged macrophyte species (e.g., M. spicatum) can improve the light climate and reduce its root growth, thereby enhancing the growth of target macrophyte species like V. denseserrulata without changes in the water quality which provides valuable insights for post-restoration lake management. Full article

There is no doubt that seagrass beds constitute one of the most productive ecosystems in shallow coastal waters. Despite this, picoplankton in seagrass ecosystems has received relatively little attention. The purpose of this study was to compare picoplankton growth and mortality rates between seagrass and unvegetated habitats using chamber incubations. We tested two main hypotheses: (i) incubation with seagrass would result in higher bacterial growth rates due to increased DOM release from seagrass photosynthesis, and (ii) Synechococcus spp. would be lower in the presence of seagrass due to competition for inorganic nutrients. Bacterial growth rates were higher in seagrass chambers (2.44 d^–1) than in non-seagrass chambers (2.31 d⁻¹), respectively, suggesting that organic carbon coming from the seagrass community may support bacterial production. Furthermore, the growth rate of Synechococcus spp. was significantly lower in the seagrass treatment than in the non-seagrass treatment, likely reflecting nutrient competition with the seagrass. Small-scale chambers proved to be a useful tool for studying the factors controlling spatial and temporal patterns of picoplankton across different habitats. Furthermore, future studies should examine picoplankton growth over a wider range of spatial scales in seagrass beds and adjacent unvegetated sediment. Full article

In recent years, floods have occurred frequently in urban and rural areas around the world, causing heavy casualties and property damage. In contrast, some traditional Chinese villages have never flooded. It is hypothesized that these villages, because of their long-term adaptations to their environment, hold necessary ecological knowledge regarding stormwater management so that damaging flooding can be avoided. Previous studies on the traditional ecological knowledge regarding stormwater management in traditional Chinese villages are mostly qualitative studies, and these fail in their evaluation of the functional performance of stormwater management facilities and measures. Therefore, we use the Storm Water Management Model (SWMM) in our quantitative evaluation of stormwater management in Zhuge, a traditional Chinese village, so as to rationally analyze the traditional ecological knowledge regarding stormwater management in traditional Chinese villages. In order to analyze the functions and efficiency of stormwater management facilities such as ponds, canals, and permeable pavement in Zhuge Village, this study sets out four scenarios: the No Pond Scenario (NO-PO), the No Canal Scenario (NO-CO), the No Permeable Pavement Scenario (NO-PP), and the actual Current Scenario (CS). The SWMM is used to simulate and quantitatively analyze the stormwater hydrological processes of the four scenarios in different rainfall return periods. The following conclusions emerged from our evaluation of the approaches used in Zhuge Village: (1) The rainwater regulation system composed of ponds, canals, and permeable pavement can play a dual role in alleviating rainstorm disasters and fully storing rainwater, achieving the flexible allocation of rainwater resources. It can effectively alleviate the problem of uneven time and space of local rainfall in shallow, hilly areas, reflecting the traditional ecological wisdom of residents in adapting to the local natural environment. (2) As a rainwater regulation device, ponds are very effective in storing water and mitigating periods of intense runoff. (3) The main function of canals is to rapidly drain water and balance rainwater resources. (4) The main function of permeable pavement is to increase rainwater infiltration and reduce the peak runoff and runoff. (5) The use of the SWMM proved effective in both quantifying the results as well as elucidating stormwater management strategies. Full article

A study was conducted to evaluate zooplankton species composition, abundance, and diversity in both natural and artificial lakes with varying trophic levels and to determine the relationship between zooplankton community structure and lake environmental conditions. This study hypothesized that correlations exist between zooplankton community structures and environmental parameters associated with eutrophication in natural and artificial lakes. Sampling was conducted across 16 distinct freshwater lentic ecosystems in Malaysia, including natural lakes/swamps, reservoirs, constructed lakes/ponds, and old mining lakes, spanning a range of trophic levels from mesotrophic to hypereutrophic conditions. Physicochemical parameters were measured in situ, while water and zooplankton samples were collected for nutrient analyses, as well as for zooplankton identification and enumeration. Throughout this study, a total of 58 zooplankton species, consisting of 36 species of rotifers, 12 species of cladocerans, and 10 species of copepods, were recorded. The highest zooplankton density (365.7 ± 13.7 ind L⁻¹) was recorded in constructed lakes/ponds while the lowest density was recorded in natural shallow lakes/swamps (200.5 ± 25.5 ind L⁻¹). On the other hand, significantly higher (p < 0.05) mean species diversity was observed in natural lakes/swamps (H’ = 2.2 ± 0.0); whereas, the lowest diversity was in old mining lakes (H’ = 1.5 ± 0.1). The canonical correspondence analysis (CCA) scores indicated that Polyarthra vulgaris and Chydorus ventricosus were the discriminating species in natural shallow lakes/swamps associated with high water transparency. Meanwhile, the small-sized cladocerans (Ceriodaphnia cornuta) and rotifers (Keratella spp., Brachionus spp., and Trichocerca spp.) were the most discriminating species in lakes with high turbidity, nutrients, and chlorophyll a concentrations, which are the main features of reservoirs and constructed lakes/ponds. Low density and diversity in old mining lakes were due to a low species number and the dominance of two species, Lophocharis curvata (38.8%) and Ptygura libera (39.7%). Overall, the high dominance of a specific zooplankton species resulted in lower biodiversity in artificial ecosystems compared to natural ecosystems. This study elucidated that zooplankton community structure in lakes was significantly influenced by the environmental conditions related to the lake trophic status. Full article

Microbial life present in the marine environment has to be able to adapt to rapidly changing and often extreme conditions. This makes these organisms a putative source of commercially interesting compounds since adaptation provides different biochemical routes from those found in their terrestrial counterparts. In this work, the goal was the identification of a marine bacterium isolated from a sample taken at a shallow water hydrothermal vent and of its red product. Genomic, lipidomic, and biochemical approaches were used simultaneously, and the bacterium was identified as Serratia rubidaea. A high-throughput screening strategy was used to assess the best physico-chemical conditions permitting both cell growth and production of the red product. The fatty acid composition of the microbial cells was studied to assess adaptation at the lipid level under stressful conditions, whilst several state-of-the-art techniques, such as DSC, FTIR, NMR, and Ultra-High Resolution Qq-Time-of-Flight mass spectrometry, were used to characterize the structure of the pigment. We hypothesize that the pigment, which could be produced by the cells up to 62 °C, is prodigiosin linked to an aliphatic compound that acts as an anchor to keep it close to the cells in the marine environment. Full article

Hebrus Valles is an outflow channel system in the plain-forming terrains of southeastern Utopia Planitia, Mars. These terrains may have formed through a combination of liquid water and volcanic processes, yet their nature, subsurface structure, and composition remain unclear. We investigate these terrains by mapping subsurface reflectors across 540 Shallow Radar (SHARAD) profiles and applying two complementary loss tangent inversion techniques. We find moderate loss tangent values across some subregions of Granicus Valles and Hyblaeus Fossae (tan δ = 0.0162 ± 0.0004 and tan δ = 0.019 ± 0.002, respectively), suggesting the presence of basaltic lava flows. We interpret non-detections in the other flows in Granicus Valles to be due to the presence of radar-lossy materials formed through aqueous processes, which supports the hypothesized occurrence of lahars in this region. A small area near Hebrus Valles exhibits subsurface reflectors with low to moderate loss tangents (tan δ = 0.010 ± 0.003), suggesting the presence of pristine lava flows or sedimentary materials capped by lava flows. We also find a widespread occurrence of very low-loss tangent materials near Hyblaeus Dorsa (tan δ = 0.0045 ± 0.0002), which may represent a lobe of the Medusae Fossae Formation or similar high-porosity materials buried underneath a lava flow. Together, these findings suggest that volcanic activity played a central role in the formation of terrains across the broader Hebrus Valles region. Full article

We here provide the first comprehensive analysis and discussion on prey consumed by the European cave salamanders of the genus Speleomantes. Our study stems from the need to shed light on the still unknown foraging behavior adopted by Speleomantes cave salamanders. Starting from the published datasets on gut contents from all Speleomantes species (including hybrids), we here discuss additional information (i.e., species ecology, lower taxonomic level), which were systematically omitted from those data sets. We analyzed a data set consisting of 17,630 records from 49 categories of consumed prey recognized from gut contents of 2060 adults and juveniles Speleomantes. Flying prey accounted for more than 58% of the prey items, while elongated crawling prey accounted for no more than 16% of the diet within a single population. Among the total recognized prey items, only three can be surely ascribed to the group of strictly-cave species (i.e., troglobites), meaning that European cave salamanders mostly forage in surface environment, and therefore represent one of the major drivers of allochthonous organic matter in subterranean environments. Some of the consumed prey seemed to be aquatic, allowing us to hypothesize whether Speleomantes are able to catch prey from a shallow body water. Furthermore, European cave salamanders possess the ability to prey upon taxa characterized by particular anti-predator defenses, while morphological constraints seem to be the most important limit to prey consumption. For each specific case, we provide insights and propose hypotheses concerning the foraging behavior that need to be tested to properly understand the foraging behavior of this cryptic salamanders. Full article

Straw, as organic material, contains macronutrients and a wide range of micronutrients. Properly treated straw can become a valuable source for soil improvement and crop nutrition needs. The field experiment was carried out at the Institute of Agriculture, Lithuanian Research Centre for Agriculture and Forestry, in 2014–2021 on Cambisol. On a shallow ploughless tillage background, eight treatments were investigated: chopped straw + ammonium nitrate (CSA), chopped straw + ammonium nitrate + NPK (CSA+F), chopped straw + microorganisms (CSM), chopped straw + microorganisms + NPK (CSM+F), straw removed, not fertilized (SR), straw removed, fertilized (SR+F), chopped straw, not fertilized (CS), chopped straw, fertilized (CS+F). We hypothesized that treatment of straw with microbiological products in combination with mineral NPK fertilizers is a more efficient technology than treatment/non-treatment of straw with ammonium nitrate, either with or without NPK fertilizers. The aim of this work was to investigate the aftereffects of seven years use of mineral NPK fertilizers and bioproducts containing soil bacteria and microscopic fungi (Bacillus megaterium, Acinetobacter calcoaceticus, and Trichoderma reesei) in combination with straw management on soil sustainability (soil C sources, soil water release characteristics, pore-size distribution, aggregate stability, crop yielding capability) and soil vitality (CO₂ exchange rate-NCER). It was revealed that NCER was highest in the treatment CSM+F (Bacillus megaterium, Acinetobacter calcoaceticus, and Trichoderma reesei + NPK). It was 32.95% higher than in CSA (chopped straw without fertilizers) and 45.34% higher than in CSA+F (chopped straw + ammonium nitrate + NPK). Bioproducts applied favored soil vitality in general by exhibiting higher soil microbiological activity. As a result, a healthy and more viable Cambisol produced a higher winter wheat grain yield. Full article