Search Results (823)

The Lhalu Wetland National Nature Reserve, the largest natural urban wetland on the Qinghai–Tibet Plateau, plays a critical role in maintaining regional ecological balance and biodiversity. However, the baseline biodiversity of this reserve remains unclear because of the extensive temporal span of historical records, shifts in taxonomic systems, and inconsistent survey methodologies, which impedes a robust scientific understanding of its ecological dynamics. This study systematically compiled and taxonomically verified species records from over 50 sources spanning the 1950s to the present. The records cover plants, fish, birds, and amphibians/reptiles, thereby resolving issues of synonyms, homonyms, and misidentifications. Each species record is annotated with its original survey time, allowing users to distinguish historically reported occurrences from those recorded in recent surveys. Species accumulation curves were constructed for major taxa and compared with 45-year climatic trends (1979–2023) and socioeconomic indicators for Lhasa City. A total of 438 vascular plant species (82 families, 251 genera) and 311 animal species (39 orders, 98 families), including 30 fishes, 174 birds, and 11 amphibians/reptiles, were documented. Invasive species comprised 55 alien plants and 13 alien fishes, while 4 plant and 46 animal species are under national protection. Temporal synchrony between increases in alien taxa and anthropogenic pressures (gross domestic product (GDP) and population growth, infrastructure development) suggests that human activities may be a potential driver of biodiversity change, but formal causal inference is precluded by heterogeneity in survey methods and sampling effort. This work provides a structured dataset of the biodiversity baseline of the Lhalu Wetland and offers a descriptive assessment of its temporal patterns in relation to climate and human disturbance, while explicitly acknowledging data limitations. It provides essential data and theoretical support for the scientific management and targeted conservation of plateau urban wetlands. Full article

The knowledge base for many small vertebrate species remains limited, largely because traditional manual data collection methods often overlook less charismatic species, such as reptiles. To address this, our pilot study harnesses open-source deep learning and markerless pose estimation technologies to evaluate the technical feasibility of tracking the spatial use and activity profiles of captive ectotherms. Specifically, we tracked these patterns over two months in a dynamically modified environment for Australian barking geckos (Underwoodisaurus milii). Our findings reveal descriptive changes in spatial occupancy and proximity across varying structural layouts. The system achieved a high raw detection accuracy (96.4%) and spatial categorization accuracy (91.7%) when validated against manual ground-truth data, confirming its robust technical performance and precision. Additionally, we automatically evaluated spatial proxies such as activity time budget, velocity, acceleration, and height usage, standardizing the analysis of extensive video recordings for nocturnal species. This pilot test introduces a simple, cost-effective method for rapid data extraction, offering a reliable, scalable monitoring solution for the management of understudied species. Full article

Fecal glucocorticoid metabolites (FGM) are widely used to assess hypothalamic–pituitary–adrenal (HPA) activity in vertebrates, but their applicability to reptiles, and more specifically to snakes, remains poorly explored. The objective of the present study was to determine how changes in housing conditions, from a standard racking system to enriched terraria, affect fecal glucocorticoid metabolite concentrations as a proxy potentially related to endocrine activity and stress response. A total of 58 fecal samples were collected across three different phases from 12 snakes representing four species: Boa constrictor (BC), Lampropeltis polyzona (LP), Pantherophis guttatus (PG), and Python regius (PR). FGM concentrations ranged from 54.9 to 832.2 ng/g, with a mean value of 298.4 ± 171.6 ng/g (mean ± SD). Data showed marked inter-individual variability, while within-individual concentrations remained relatively stable across experimental phases. PG exhibited the highest FGM concentrations during the study, with LP showing intermediate levels and PR and BC the lowest. The results showed that housing conditions did not have a significant effect on FGM concentrations in the studied population. In conclusion, the present study showed that fecal hormone analysis provides an integrated measure of glucocorticoid metabolites concentrations over time, potentially reflecting physiological differences among snake species. Full article

In the context of global warming, the resulting persistent thermal stress has become a critical environmental factor influencing the structural and functional homeostasis of gut microbiota in reptiles. In this study, Eremias roborowskii, a desert lizard endemic to the extreme heat conditions of the Turpan Basin, was selected as an ideal model for evaluating the ecological impacts of global warming. Meanwhile, a 60-day controlled laboratory experiment was conducted, exposing the lizards to normal (30 °C ± 1 °C), elevated (37 °C ± 1 °C), and high (42 °C ± 1 °C) temperatures to reflect future climate scenarios. Using shotgun metagenomic sequencing, the gut microbiota was characterized to investigate the dynamics of the antibiotic resistance genes (ARGs) and carbohydrate-active enzymes (CAZymes) under heat stress. The results reveal that elevated temperature selectively promotes heat-tolerant gut microbiota, such as Tetragenococcus and Faecalicatena, by altering host energy metabolism and modulating heat stress adaptation to maintain intestinal homeostasis. Moreover, the observed increase in resistome diversity and richness under elevated temperature may be attributed to temperature-induced shifts in gut microbial composition, particularly the enrichment of heat-tolerant ARG-carrying bacterial taxa. Metabolic changes in CAZymes were caused by gut microbiota remodeling, which optimized carbon utilization and preferentially allocated cell wall synthesis and repair. Furthermore, the pentose phosphate pathway and amino acid biosynthesis pathways were upregulated, providing NADPH for antioxidant defense and precursors for protein synthesis, respectively, thereby contributing to the maintenance of microbial cellular homeostasis. Our study provides a theoretical basis for understanding functional gene adaptation strategies in wildlife microbiomes due to climate change. Full article

Background: The Interleukin-6 (IL6)–Interleukin-6 receptor (IL6R)–Janus kinase (JAK)–signal transducer and activator of transcription (STAT) signaling pathway plays a crucial role in innate immunity by inducing the production of various immune effectors. However, its stepwise evolutionary assembly across metazoans remains incompletely characterized. Methods: This study systematically screened 18 metazoan phyla or subphyla for the presence of IL6 family cytokines, their receptors, JAKs, and STATs. Phylogenetic relationships were constructed using Neighbor-Joining methods, and domain architectures were predicted via SMART. Results: IL6 was found only in vertebrates, whereas IL6R could be traced to Mollusca. JAK and STAT appeared early in Porifera. IL6R, JAK1/2, STAT1 and STAT5A/B first co-existed in Mollusca. The IL-6 co-receptor gp130 was detected in fish, but was not reliably detected in available reptiles’ and birds’ genomes. Conclusions: The IL6-IL6R-JAK-STAT pathway was assembled incrementally: JAK and STAT were first detected in Porifera, IL6R was added in Mollusca to form the IL6R-JAK-STAT module, and finally, most IL6 family cytokines were present in vertebrates (fish). These findings might provide a pre-existing molecular framework that facilitated the subsequent evolution of the complete pathway in vertebrates. Collectively, this study provides a comprehensive evolutionary framework for the stepwise assembly of the IL6-IL6R-JAK-STAT signaling pathway across metazoans. Full article

Background/Objectives: Non-tuberculous mycobacteria (NTM) are increasingly recognized as important opportunistic pathogens at the human–animal–environment interface. Their growing relevance is driven by increasing disease burden, environmental persistence, occurrence in multiple animal hosts and complex antimicrobial resistance (AMR) patterns. Unlike classical zoonotic pathogens, most NTM are primarily acquired from shared environmental reservoirs rather than through sustained host-to-host transmission. This review examines NTM from a One Health perspective, focusing on AMR, ecology, animal occurrence, cross-species exposure and public health relevance. Methods: A narrative review of the current literature was conducted to synthesize evidence on the ecology, environmental reservoirs, occurrence in animals, transmission patterns and AMR mechanisms of NTM. Particular attention was given to studies addressing the human–animal–environment interface and the implications of NTM for One Health surveillance and risk assessment. Results: The reviewed literature shows that NTM are widely distributed in water, soil, sediments and biofilms, creating repeated opportunities for exposure in both animals and humans. They have been reported in livestock, wildlife, companion animals, reptiles and aquatic organisms, where they may act as colonizers, opportunistic pathogens, or sources of diagnostic interference. Evidence for direct animal-to-human transmission remains limited, but animal and environmental findings are important for understanding ecological overlap, host range and circulation of resistant strains. AMR in NTM is shaped by intrinsic resistance, acquired mutations, efflux activity, and biofilm-associated tolerance, which together complicate treatment and resistance prediction. Conclusions: NTM should be considered environmentally maintained, multi-host organisms of increasing One Health importance rather than conventional zoonotic pathogens. Improved interdisciplinary surveillance, diagnostics and research are needed to clarify exposure pathways, resistance development and public health risk. Full article

Kenya has a long history of both legal and illegal wildlife trade, functioning as a source, consumer, and transit hub within global wildlife markets. Yet, despite its increasing prominence, the scale and composition of Kenya’s captive-bred and ranched wildlife trade sectors remain poorly characterized, particularly following the enactment of the Wildlife Conservation and Management Act (WCMA) in 2013. This study addresses this gap by analyzing Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) annual report data from 2013 to 2023 to: (1) identify trends in the volume and taxonomic composition of CITES-listed species exported as captive-bred or ranched from Kenya; (2) quantify the number of specimens and individuals traded; (3) assess their conservation status and legal classification; and (4) identify key export destinations. Between 2013 and 2023, Kenya reported 886 CITES export records involving captive-bred and ranched specimens from 28 vertebrate taxa across nine orders. Reptiles dominated exports (81% of records), followed by birds (15%) and mammals (4%). Live animals accounted for 80% of records (with reptiles comprising 96.1% of all live animal export records), totaling more than 870,000 individuals traded over the study period. The annual number of export records more than doubled across the decade, while exports of individual live reptiles increased more than tenfold, from 8551 individuals in 2013 to 86,330 in 2023. Most exports were commercial (93%) with the United States, Germany, Spain, Taiwan, and the Republic of Korea identified as major importers. 77% of exported species have unknown or declining wild population trends, and seven species are internationally threatened, including the Critically Endangered pancake tortoise (Malacochersus tornieri). We highlight the animal welfare, conservation, and biosecurity implications of this rapidly expanding trade, as well as consistent discrepancies between exporter- and importer-reported quantities that indicate substantial monitoring and regulatory challenges. The findings provide timely evidence to inform national wildlife management and protection measures, and ongoing policy discussions including those surrounding the forthcoming Wildlife Conservation and Management Bill (2025) and the role of captive breeding in Kenya’s wildlife trade. Full article

Reptiles have varied lung anatomy with three main lung types: unicameral, transitional, and multicameral. Crocodilians have a well-developed bronchial tree, while all other species have either a limited number of bronchi or no bronchi at all. The primary gas exchange structures are the faveoli and ediculae, which can have either homogeneous or heterogeneous distribution within the lung parenchyma. The physiology of reptile respiration is also complex, varied, and at times contradictory across species. A basic understanding of anatomy and physiology is essential for proper diagnostic and therapeutic approaches to reptile patients. Therefore, a paradigm shift is needed to start thinking of the anatomy and physiology of individual species rather than believing that a single concept applies to all reptiles equally. As such, future research should aim to duplicate concepts across the more common species encountered in clinical practice. Full article

Salmonella spp. is a major zoonotic pathogen. Although reptiles are mostly considered subclinical carriers, clinical disease may develop following immunosuppression. Clinical salmonellosis in reptiles has been extensively reported; however, the condition has been rarely described in bearded dragons (Pogona vitticeps). We retrospectively analyzed six cases of salmonellosis in bearded dragons and characterized the pathological, immunohistochemical, and bacteriological findings. Clinical signs and gross findings were mostly non-specific. Histological findings mainly consisted of fibrinonecrotizing enterocolitis (83.3%); necrotizing or granulomatous hepatitis (66.7%); pneumonia including bronchopneumonia or interstitial pneumonia in one case each (33.3%); tubulointerstitial nephritis with tubular necrosis (16.7%); and coelomitis (16.7%). Salmonella enterica subsp. houtenae was cultured in three cases (33.3%), whereas S. enterica subsp. enterica serovar Rissen, S. enterica subsp. enterica serovar Cotham, and S. enterica subsp. diarizonae were cultured in one case each. Intralesional bacteria were detected via immunohistochemistry in kidneys and colon in two cases (33.3%). The predominance of lesions in the intestines and liver likely reflects initial intestinal colonization followed by hematogenous dissemination to the liver. Hepatic lesions are thought to represent different stages along a continuum, progressing from acute necrosis to discrete granuloma formation. Renal and respiratory involvement was infrequent, as reported in other reptile species. Some of the isolated Salmonella subspecies (S. diarizonae and S. houtenae) are well-recognized causes of clinical disease in other reptile species but not previously identified in bearded dragons. This study provides a comprehensive pathological, immunohistochemical, and bacteriological characterization of salmonellosis in bearded dragons, thus raising awareness and assisting in the identification of this condition. Full article

The Chinese giant salamander (Andrias davidianus) is a nationally protected species in China, and its respiratory behavior serves as a key indicator of its physiological state, health status, and biological rhythm. However, research on intelligent monitoring of its respiratory behavior remains limited due to several challenges, including the species’ nocturnal habits, resulting in low image contrast and poor quality in dark environments; extremely subtle breathing movements; and high-cost manual annotation, leading to a scarcity of high-quality annotated visual data. These factors severely constrain the application of deep learning techniques in this field. To support research on respiratory behavior monitoring in the Chinese giant salamander, this study constructs and releases the CGS-BR dataset, which is the first vision-based dataset dedicated specifically to respiratory behavior detection in this species. The dataset was collected under controlled simulated breeding conditions and consists of 1732 images extracted from 215 high-definition video clips. Following a standardized procedure, each complete respiratory cycle is manually annotated into four stages: head-up, diving, exhalation, and inhalation. To validate the effectiveness of this dataset, this study selects YOLOv8n as the baseline model, which balances detection accuracy, speed, and parameter count, enabling efficient giant salamander respiratory detection under limited resources. By comparing it with several representative models, we provide a reliable evaluation of the dataset’s applicability. CGS-BR aims to provide fundamental data support for research on respiratory monitoring in the Chinese giant salamander, laying the foundation for subsequent applications in conservation management, captive breeding, health monitoring, and early disease warning. Full article

Solitary living is an evolutionarily widespread yet comparatively under-theorized social system, despite its occurrence across diverse animal taxa. Shrikes (family Laniidae) are small predatory passerines that combine raptorial behavior, strong territoriality, and predominantly solitary space use, making them a powerful model for examining the ecology and evolution of solitary living. Here, I synthesize published work on shrike behavioral ecology and explicitly link these traits to the costs and benefits of a solitary lifestyle. I argue that shrikes exemplify how solitary species can offset the absence of social buffering through cognitive innovation, finetuned habitat selection, and flexible yet tightly bounded sociality. I then compare shrike ecology to solitary mammals and reptiles, highlighting convergent patterns in resource dispersion, spatial memory, risk management, and juvenile dispersal. I further examine how anthropogenic pressures, such as habitat fragmentation, climatic instability, and urbanization, interact with solitary life histories and review evidence from management interventions in both European farmland and North American systems that demographic recovery is achievable but remains contingent on addressing broader land-use conflicts and sources of adult mortality. Finally, I outline five interconnected research priorities—spanning cognitive ecology, trophic interactions, movement ecology, genomics, and formal comparative analyses—that would move shrike research from its current observational foundation toward a more experimental, mechanistic, and phylogenetically informed programme. By reframing shrikes as a model taxon for solitary living, this review aims to integrate avian behavioral ecology into broader comparative frameworks of social organization, cognition, and resilience under global change. Full article

Introduction of species represents today one of the most important problems of nature conservation. Special attention is paid to alien vascular plants and vertebrates. In the Afrotropical Region (sub-Saharan Africa), however, there is a lack of comprehensive review of alien amphibians and reptiles. The presented paper constitutes an attempt to overview the status, distribution and threats posed by introduced herp species to sub-Saharan Africa since the second half of the 18th century. This review includes 21 amphibian (including 10 established) and 57 reptile (including 33 established) species introduced to sub-Saharan Africa. Most species introduced to sub-Saharan Africa which subsequently developed viable populations originated from the Malagasy (32%), Afrotropical (30%), and Oriental (27%) Regions. Most introductions were made in the last two decades, mostly as results of an increase in international trade and herp pet industry, especially in South Africa. Stowaway and pet trade are the most common pathways of introductions. Several factors determine the successful establishment of introduced alien herp species in sub-Saharan Africa, viz. behavioral and morphological traits, propagule pressure, climate and habitat overlap, and presence of potentially competing species. The impact of alien herps in sub-Saharan Africa on the local biodiversity is not well investigated. In comparison with other continents the number of introduced and established herp species in sub-Saharan Africa is relatively low. The Malagasy Region has the highest number of introduced herp species in sub-Saharan Africa. Full article

The primary aim of wildlife rehabilitation centres is the release of treated animals back into the wild after treatment. Data collected during rehabilitation can provide valuable insights into current trends and can help with conservation strategies aiming to reduce human-related negative impact. This review analyses records from past and currently operating wildlife rescue and rehabilitation centres in Lithuania over a 23-year period. Data were compiled for 7847 individual animals representing 216 species of birds, mammals, and reptiles. The study evaluates patterns of seasonal admission, taxonomic composition, known causes of injury or admission, and rehabilitation outcomes. The results showed that 83% of cases involved birds, mammals comprised 16%, and reptiles were recorded only infrequently (0.52%). Admissions peaked in summer, comprising 42% of all cases. Injuries of unknown origin were the most common (55%). Among cases with identified causes, a substantial proportion were associated with human activities, including road accidents (5% of all cases), collisions with anthropogenic structures (4%), and attacks by domestic cats or dogs (3%). The presence of a specialized rehabilitation centre, together with active public involvement in the rescue of injured wildlife, contributes to release rates reaching approximately 30% of admitted animals. Datasets such as those analyzed in this study may contribute to improved preparedness for managing unavoidable human–wildlife interactions in the future. Full article

Accurate sex identification in reptiles with genotypic sex determination is essential for breeding management, veterinary care and evolutionary research, yet commonly used methods are often invasive, stressful or unreliable. This study aimed to evaluate a dosage-based quantitative PCR approach for molecular sex determination in caenophidian snakes, using naturally shed epidermal skin as a non-invasive DNA source. Genomic DNA extracted from shed skin was analysed by qPCR targeting conserved Z-linked genes (ADARB2, ARMC4 and TANC2), together with autosomal and reference genes, to assess sex-specific differences in gene copy number. Sixteen caenophidian snake species were examined, including taxa for which molecular sexing data are currently scarce or unavailable. The autosomal control gene showed dosage ratios close to parity between sexes, supporting DNA quality and reference gene reliability; meanwhile, Z-linked markers generally exhibited reduced dosage in females relative to males, consistent with a ZZ/ZW sex determination system. These results demonstrate that dosage-based qPCR applied to shed epidermal skin provides a promising and non-invasive framework for molecular sex determination in caenophidian snakes, without compromising animal welfare. Full article