Search Results (225)

Storage duration critically shapes the characteristic sweet and mellow quality of ripened pu-erh tea (RPT), yet the underlying chemical mechanisms remain poorly understood. This study investigated the sensory and chemical evolution of a representative commercial RPT product across a nine-year storage gradient (1, 3, 5, 7, and 9 years) by integrating Quantitative Descriptive Analysis (QDA), chromaticity measurement, targeted quantification of 42 non-volatile components, and Headspace Gas Chromatography–Mass Spectrometry (HS-GC-MS) volatilomics with multivariate statistical modeling. Prolonged storage drove systematic sensory maturation: the stale aroma gradually purified, and the taste profile transitioned significantly from heavy and mellow to sweet and mellow (p < 0.05), accompanied by a deepening infusion color with increased redness and yellowness indices. Targeted chemical profiling revealed significant decreases in total polyphenols and astringent esterified catechins, particularly epicatechin gallate (ECG) and epigallocatechin gallate (EGCG) (p < 0.05), while theabrownins remained stable and soluble sugars peaked at intermediate storage stages. Pearson correlation analysis linked these chemical shifts to sensory perception, with enhanced sweetness, mouthfeel thickness strongly associated with reduced monomeric catechins and free amino acids (p < 0.001). Volatilomics combined with K-means clustering and relative odor activity value (ROAV) analysis revealed a dual mechanism of flavor refinement: progressive accumulation and increasing odor activity of aged aroma markers (1,2,3-trimethoxybenzene, β-ionone) coupled with systematic attenuation of pungent acids and grassy aldehydes. These findings, based on a single, standardized commercial product, elucidate the chemical-sensory foundation of the sweet and mellow profile in aged RPT and provide candidate markers and a transferable analytical framework for quality assessment of stored teas. Full article

This review evaluates pretreatment strategies for blending the organic fraction of municipal solid waste (OFMSW) with agricultural residues to recover fermentable sugars. Three mechanistic benefits have been hypothesized for such blends: ash-mineral pH buffering, endogenous protein reduction of non-productive cellulase–lignin binding, and inhibitor dilution. These mechanisms are inferred from analogous lignocellulosic systems rather than measured directly in OFMSW–agricultural residue combinations, and their translation into saccharification gains remains substrate- and pretreatment-specific. A synergy index framework with a four-tier classification (true synergy, additive, substitution, and process complementarity) is applied to reclassify the available evidence, alongside an assessment of pretreatment chemistry, enzymatic hydrolysis outcomes, and techno-economic feasibility. Integrated sequential pretreatment, particularly acid-catalyzed steam explosion and deacetylation with mechanical refining, proved most robust for heterogeneous feeds. The strongest Tier I synergy is found for SO₂-catalyzed steam explosion of hybrid poplar–wheat straw (SI 1.29–1.33; 22% monomeric sugar gain). OFMSW combined with organosolv beechwood cellulose at 35–45% OFMSW reached 58–68% saccharification (44–46 g sugar L⁻¹), a Tier III–IV outcome. Matched-control saccharification data for OFMSW–agricultural residue blends specifically have not been reported. Co-processing corn stover with wet organic waste reduced CO₂ mitigation cost from $236 to $67 per ton CO₂-eq under bio-CNG upgrading. Formal synergy quantification, blend-specific inhibitor profiling, and high-solids process intensification are the central prerequisites for commercial translation. Full article

Background: Attention-deficit/hyperactivity disorder (ADHD) is a prevalent neurodevelopmental condition with psychosocial impact. Dietary carbohydrates, particularly added sugars, refined starches, and high-glycemic index/load (GI/GL) patterns, have been proposed as modifiable exposures that may relate to attention and behavioral regulation. This systematic review synthesized evidence linking carbohydrate quantity and quality to ADHD-related outcomes. Methods: Following PRISMA 2020, PubMed, Scopus, and Web of Science were searched for English-language studies published from January 2015 to December 2025. Eligible studies included observational and interventional designs in children, adolescents, or adults with a clinical ADHD diagnosis or validated symptom assessment. Risk of bias was assessed using NOS/NOS-adapted criteria, RoB 2, and ROBINS-I. Findings were synthesized narratively by exposure domain. Results: Of 1174 records identified, 48 studies were included: 38 observational and 10 interventional. Sugar-related exposures showed the most consistent pattern: 15 of 16 studies examining added sugars, sugar-sweetened beverages, sweets, candy, or sweet dietary patterns reported positive associations with ADHD diagnosis, symptom severity, hyperactivity, or less favorable ADHD-related outcomes. Findings for total carbohydrate intake were inconsistent. GI/GL-specific evidence was limited but generally adverse in direction. Among intervention studies, symptom improvement after modification was reported in 6 of 10 studies, whereas 4 studies showed mixed, preliminary, non-significant, or non-superior findings. Most observational studies showed moderate to high risk of bias, while interventional studies showed variable risk across domains. Conclusions: Poorer carbohydrate quality may be associated with greater ADHD-related symptom burden, whereas total carbohydrate intake showed inconsistent associations. Certainty remains limited by heterogeneity, residual confounding, risk of bias, and limited carbohydrate-specific intervention evidence. Full article

Preharvest selenium (Se) biofortification is a promising strategy to enhance both the nutritional value and postharvest performance of vegetables. However, its effects on cherry tomato quality during storage, particularly in relation to ripening stage at harvest, remain poorly understood. This study evaluated the impact of foliar Se application (0.5 mM, as Na₂SeO₄) on carpometric, compositional, and functional traits of cherry tomatoes harvested at two ripening stages (orange-red and deep red) and stored for 0, 10, and 20 days at 11.0 ± 0.5 °C. The Se application increased fruit Se concentration (∼30-fold) and improved dry matter (+8.1%) and firmness (+8.3%) throughout storage. At the end of storage, all fruits showed reduced firmness (up to −44%) and increased fresh weight loss (up to 8.5%), although Se-biofortified fruits consistently maintained a higher dry matter content. The effects of Se on compositional traits were ripening stage-dependent, as it enhanced glucose (+8.2%), fructose (+10.0%), and total sugars (+9.4%) in fully ripe fruits, while increasing titratable acidity in less mature ones (+8.2%). Moreover, Se reduced total carotenoids in fully ripe fruits (−13.2%) but increased ascorbic acid during storage (+19.4%), irrespective of ripening stage. Overall, Se biofortification effectively enriched cherry tomatoes and modulated their postharvest behavior. However, the contrasting, stage-dependent effects of Se biofortification on the functional compounds of cherry tomatoes emphasize the need to refine the biofortification strategy in order to achieve a more consistent and comprehensive improvement in fruit quality. Full article

This review comprehensively examines the structural architecture, catalytic mechanisms, and targeted molecular engineering of α-amylase (primarily the GH13 family), a pivotal biocatalyst in the food industry. We highlight diverse microbial sources of α-amylases and their cost-effective heterologous expression in well-characterized hosts like Bacillus subtilis and Escherichia coli. To overcome extreme operational bottlenecks—such as elevated temperatures and acidic environments—recent advances in protein engineering are critically evaluated. These strategies, including directed evolution, semi-rational design, and advanced immobilization on nanomaterials, synergistically enhance the enzyme’s thermostability, catalytic efficiency, and reusability. Furthermore, this paper synthesizes the state-of-the-art applications of engineered α-amylases across key food processing sectors, including baking, sugar refining, and brewing. By integrating structural biology with advanced material science, this review provides a targeted roadmap for developing next-generation, high-performance α-amylases to address current and future challenges in sustainable food processing. Full article

Background: The Mediterranean diet (MD) represents a nutritionally balanced eating pattern characterized by high consumption of fruits, vegetables, legumes, nuts, whole grains, olive oil, fish, and extra-virgin olive oil as the principal fat source and limited intake of red meat and refined sugars. Emerging evidence indicates that the MD’s anti-inflammatory and antioxidant properties extend beyond systemic health, potentially reducing the risk and severity of periodontitis. This narrative review aimed to synthesize current evidence on the relationship between adherence to the MD and periodontal health outcomes. Methods: A comprehensive electronic literature search was conducted in PubMed without restrictions on publication date. Fourteen studies, ranging from 2019 to 2025, were included, encompassing human, clinical, experimental, and review designs that examined MD adherence and its effects on periodontal parameters. Eligible studies included cross-sectional, cohort, randomized controlled trials; systematic reviews; and animal models assessing clinical periodontal indices, inflammatory biomarkers, or microbial composition. Extracted data included study design, population characteristics, dietary assessment methods, and primary periodontal findings. Results: Most studies demonstrated that greater adherence to the MD was associated with improved periodontal parameters, including reduced probing pocket depth, clinical attachment loss, and bleeding on probing. Interventional trials showed significant reductions in systemic inflammatory markers such as IL-1β, TNF-α, and CRP, along with decreased counts of periodontopathogenic bacteria. Experimental studies further revealed the protective role of oleic acid and polyphenols in regulating macrophage activity, suppressing osteoclastogenesis, and enhancing IL-10 expression via epigenetic modulation. However, heterogeneity in dietary scoring systems, sample characteristics, and follow-up duration limited direct comparison, and not all associations reached statistical significance. Conclusions: Current evidence supports a beneficial association between MD adherence and periodontal health, mediated through anti-inflammatory, antioxidant, and microbiome-stabilizing mechanisms. Further standardized longitudinal and interventional studies are needed to confirm causality and refine nutritional strategies for periodontal disease prevention and management. Full article

Background: Mechanistic evidence increasingly implicates fructose exposures as contributors to the development and exacerbation of asthma and other atopic diseases. Proposed mechanisms include gut dysbiosis, impaired epithelial barrier integrity in the gut and airways, metabolic endotoxemia, and amplification of type 2 immune responses. However, epidemiologic findings linking fructose intake with asthma and atopic disorders remain heterogeneous. Objective: To conduct a review of epidemiologic studies evaluating associations between dietary fructose-containing exposures and atopic outcomes in pediatric populations. Methods: A systematic search of PubMed and Embase identified cohort, case-control, cross-sectional, and randomized feeding studies assessing fructose exposure in relation to asthma and atopic outcomes in pediatric populations. Eligibility screening, data extraction, and risk-of-bias assessment were conducted by one reviewer and confirmed by the other. Results: Seventeen epidemiologic studies met criteria. Multiple cohorts (e.g., BRISA, PIAMA) reported modest to moderate associations between higher sugar-sweetened beverage (SSB) intake and pediatric asthma or “asthma traits.” Cross-sectional analyses from NHANES and the National Children’s Study showed stronger associations, with greater fructose exposures linked to two- to five-fold higher odds of asthma. High fructose beverage consumption demonstrated the most consistent positive associations. Large ISAAC-based studies reported largely null findings, reflecting broad dietary exposure categories and limited specificity for fructose-rich beverages. Evidence for rhinitis, eczema, and sensitization was directionally consistent. Conclusions: Despite heterogeneity, the convergence of mechanistic plausibility with epidemiologic signals supports a potential contributory role of high fructose exposure in pediatric atopic disease. More rigorous longitudinal studies with biomarker-based exposure assessment are needed to refine causal inference. Full article

The large-scale production of microbial bioplastics remains limited by high production costs, reliance on refined substrates, and inefficient utilization of agro-industrial residues. Although sugarcane bagasse has been explored as a carbon source for polyhydroxyalkanoate production, studies have predominantly focused on poly (3-hydroxybutyrate) (PHB), with limited reports on copolymer synthesis from pentose-rich lignocellulosic streams. In this study, a newly isolated Bacillus sp. HLI02 was employed for the biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), using pentosan-rich sugarcane bagasse hydrolysate as an inexpensive and sustainable carbon source. Fermentation parameters were systematically optimized at different pH and temperature, and the strain demonstrated efficient conversion of xylose-rich hydrolysate into PHBV without the requirement for external nutrient supplementation. Under optimized conditions (pH 7.0, 37 °C, and C/N ratio of 40), a maximum PHBV yield of 2 g/L, corresponding to 59.5% of cell dry weight, was achieved. Structural and compositional analyses using Fourier transform infrared spectroscopy (FTIR), gel permeation chromatography (GPC), and ¹H and ¹³C nuclear magnetic resonance (NMR) spectroscopy confirmed successful PHBV copolymer formation with well-defined structural characteristics. Thermal analysis revealed a decomposition temperature of 166 °C, indicating good thermal stability. The produced PHBV further exhibited favourable biocompatibility and biodegradability, supporting its potential applicability in sustainable packaging and related sectors. This work demonstrates the effective conversion of hemicellulosic sugarcane bagasse hydrolysate into PHBV using a newly isolated Bacillus strain, highlighting an underexplored route for copolymer production from agro-waste–derived C5 sugars. By integrating low-cost feedstock utilization with process optimization and comprehensive polymer characterization, this study contributes to the development of economically viable and sustainable bio-based polymer production strategies. Full article

Sustainable xanthan gum (XG) production is increasingly prioritized as global demand rises, and conventional processes face economic and environmental constraints. Traditional manufacturing depends heavily on refined sugars, intensive fermentation control, and solvent-based purification, which elevate production costs and ecological impact. This review highlights recent advancements designed to improve sustainability across the XG value chain, focusing on alternative substrates, optimized fermentation, and greener extraction methods. Agricultural residues, food-processing waste, lignocellulosic biomass, and industrial effluents have emerged as promising low-cost substrates that reduce reliance on refined sugar sources while supporting waste valorization. Pretreatment strategies, such as acid hydrolysis, enzymatic processing, and integrated biological–chemical methods, significantly enhance the accessibility of complex biomass for microbial fermentation. Concurrently, improvements in strain selection, metabolic engineering, and process control have increased XG yield, molecular weight, and rheological performance. Environmentally friendly extraction technologies, including ultrasound-assisted extraction, pulsed electric fields, membrane filtration, and electro-dewatering, further reduce solvent consumption and energy demand in downstream processing. However, challenges persist, including substrate variability, formation of inhibitory compounds, strain instability, and regulatory considerations for waste-derived substrates or genetically modified strains. Future progress will rely on integrating bioprocess intensification, genetic engineering, and techno-economic assessment to build scalable, low-impact, and circular XG production systems. Full article

Background/Aim: Dental caries is a multifactorial disease influenced by dietary habits, lifestyle factors, and host biochemical processes. Oxidative stress and advanced glycation end products (AGEs) have been implicated in oral and systemic pathophysiology, but their combined association with caries experience remains unclear. This study aimed to evaluate the relationships between caries indices, diet, smoking, oxidative stress markers, and AGEs in adults. Materials and Methods: A cross-sectional study was conducted on adults enrolled in the SALIVAGES project (2018–2020). Dental status was assessed using the DMFT index. Dietary habits and smoking status were recorded using a validated questionnaire. Oxidative stress parameters (TAC, TOS, OSI, NO, MDA, total thiols) and AGEs (FruLys, MG-H1, CML, CEL, Pyr, Arg, Lys) were quantified in saliva and plasma. Associations were analyzed using correlation tests and multivariable regression models (α = 0.05). Results: The mean DMFT was 21.89 ± 7.13, with missing teeth predominating. Caries experience was significantly associated with oxidative stress, AGEs, diet, and lifestyle. Higher decay scores were associated with increased NO and total thiols and reduced antioxidant capacity. Several salivary AGE-related biomarkers (FruLys, MG-H1, CML, and CEL) were negatively associated with the decay index. Sugary beverages, refined carbohydrates, pastries, and donuts were strongly positively associated with the decay index, whereas wholemeal bread showed an inverse association with caries indices. Smoking was independently associated with higher decay and DMFT values, corresponding to an approximately three-unit higher DMFT score. Conclusions: Caries experience in adults is associated with dietary, lifestyle, and biochemical factors. Sugar intake and smoking showed the strongest associations with caries indices, while oxidative stress parameters and selected salivary AGE-related biomarkers showed weaker but significant inverse associations with decay. These findings support preventive strategies targeting diet quality, smoking cessation, and redox balance to reduce oral disease burden. Full article

Background/Objectives: Meals eaten at movie theaters may have acute, negative health effects due to high refined sugar and moderate sodium content. We aimed to characterize the cardiometabolic response to movie-theater-style meals independently (fasting) and after high-fat meal consumption. Methods: Participants (N = 10; 5M/5F; 18–45 y) completed two meal trials (randomized). At both trials, participants ate a movie-theater-style meal (popcorn, candy, and soda; 884 kcal, 150 g sugar, and 700 mg sodium). At one trial, the movie theater meal was consumed while fasting (Fasting Trial). At the other trial, a high-fat meal (820 kcal; 56 g fat) was consumed 3.5 h prior to the movie theater meal (Fed Trial). Blood was collected (0, 0.5, 1, 2, 3, and 4 h) and endothelial function (i.e., flow-mediated dilation or FMD) was assessed (0, 2, and 4 h) at both trials. Serum metabolic markers (glucose, insulin, triglycerides, and HDL-C) and biomarkers of intestinal permeability (sCD14 and LBP) were measured. Mixed-model ANOVAs (meal × time) and change scores (Δ) were used to compare responses between trials. Results: At both trials, glucose, insulin, and triglycerides increased, while HDL-C decreased (p_time’s ≤ 0.05). ΔInsulin (p = 0.02), but not Δglucose, was higher at Fasting versus Fed. Peak glucose (range = 86–178 mg/dL) and insulin (range = 28.3–307.6 mU/L) were highly variable between participants across trials. Absolute and percent FMD tended to decrease, regardless of trial (p_time’s ≥ 0.08). Conclusions: Overall, the movie theater meal impacted a number of cardiometabolic factors when consumed independently and after a high-fat meal, although there was notable inter-individual variability. Full article

The prevalence of diet-related noncommunicable diseases (NCDs; e.g., obesity, type 2 diabetes, cardiovascular disease, and certain cancers) is increasing globally, while food systems are also driving climate change and biodiversity loss. Transitioning to predominantly plant-based (“plant-forward”) dietary patterns can improve health and lower environmental impacts. We present the Slovenian Nutrition Guidelines 2025 (SNG2025)—their methodology, development, and core recommendations. Developed as adult food-based dietary guidelines, the SNG2025 are evidence-informed, drawing on the scientific literature, national nutritional data, and expert consensus. We set quantitative daily intake targets by integrating evidence on primary NCD outcomes with environmental metrics (greenhouse gas emissions, land use, and water use), which led to upper limits for animal-based foods. The recommended plant-forward dietary pattern, aligned with the EAT–Lancet planetary health diet, emphasises vegetables, fruits, legumes, whole grains, nuts, seeds, and unsaturated oils; allows low-to-moderate amounts of seafood, poultry, dairy, and eggs; and keeps red and processed meat, free sugars, refined grains, saturated fat, salt, ultra-processed foods (UPFs), and alcohol to a minimum. For the first time, we operationalise health, environmental sustainability, and equity (cultural diversity and accessibility) through a three-tier, plant-forward food plate model (Mediterranean, vegetarian [lacto-ovo], and whole food, plant-based [vegan]). The SNG2025 aim to reduce the risk of NCDs, lower the dietary environmental footprint, and improve fair access to healthy food. They signal a shift from disease management to a prevention-oriented, systems approach that aligns health and ecological goals. With robust implementation, supportive policies, and multisector collaboration, the SNG2025 can strengthen population health, foster more resilient food systems, and advance equity and long-term sustainability. Full article

Background/Objectives: The global prevalence of overweight and obesity among young adults has doubled since 1975, primarily due to unhealthy dietary habits and sedentary lifestyles. Understanding dietary patterns (DPs) in this population is essential for designing effective prevention strategies. This study aimed to characterize the dietary patterns and diet quality of university students and to examine their physical activity and associated health risks. Methods: A convenience sample of 136 participants (77.9% females, 22.1% males) was recruited. Data on clinical history, lifestyle behaviors, and physical activity were collected using a structured questionnaire. Dietary intake was assessed using a food frequency questionnaire and three 24-h dietary recalls. Intake was analyzed by food groups, total energy, and macronutrient and fiber composition. Principal component analysis was applied to identify DPs. Results: Three major DPs were identified: Ultra-Processed Foods, Variety Foods, and Traditional Mixed Mexican. Overall, participants showed low consumption of fiber, legumes, and nuts, coupled with high intake of animal-based foods. The mean daily energy intake was 2278 kcal for men and 2008 kcal for women. Although participants demonstrated higher adherence to the Traditional Mixed Mexican pattern, a strong tendency toward the Ultra-Processed Foods pattern was observed, which is linked to an increased risk of chronic diseases and poor nutritional outcomes. Conclusions: The findings highlight the urgent need for targeted dietary interventions among university students. Strategies should emphasize increased intake of fiber-rich plant foods, moderation of protein consumption, and reduction in refined carbohydrates and added sugars to promote healthier dietary habits and prevent chronic disease development. Full article

Although plant-based convenience foods have gained significant market share, many are high in fat, salt, and sugar while low in nutrients. The current study aimed to develop a vegan oyster mushroom soup powder enriched with moringa, mung bean, and pumpkin. These ingredients were chosen for their high nutritional value and availability. Four soup formulas, each containing varying amounts of moringa (0%, 1%, 2%, and 3%), were prepared, and a sensory evaluation, proximate analysis, and total aerobic plate count were carried out. The 1% moringa formulation showed the highest consumer acceptance. In this formula, moisture, ash, protein, fat, fiber, carbohydrate, and energy content were reported as 13.6%, 7.6%, 16.3%, 2.2%, 9.8%, 50.5%, and 287 kcal/100 g, respectively. The novel powdered soup product had higher amounts of phenolic compounds, total antioxidants, and iron compared to local, commercially available equivalents. Total aerobic plate counts remained below 10⁵ CFU/g; a common acceptability limit for dried soups, throughout the 4-month storage study under ambient conditions. Overall, the developed soup powder demonstrated superior nutritional quality and could support consumers in meeting their daily nutrient requirements. With further refinement, particularly by optimizing the drying process to better retain heat-sensitive nutrients, this product shows potential as an affordable and nutritious option to address inadequate protein intake and iron deficiency in Sri Lanka. Full article

Archaeological research on Santiago Island (Cape Verde) offers a strategic framework for investigating ceramic material culture shaped by Iberian and African interactions during the early modern period. This study presents first-stage results from a non-destructive archaeometric analysis of pottery fragments recovered from early colonial sites and curated at the Museu de Arqueologia in Praia. Using portable X-ray fluorescence spectroscopy (pXRF), low-fired, handmade vessels associated with African technological traditions were analysed to determine their elemental composition and potential provenance. The work also focused on sugar moulds, containers used in the refining of this product, one of the most important in Atlantic colonisation. The resulting geochemical data is compared with established reference groups from the Iberian Peninsula, Atlantic Africa, and Macaronesia. Elemental variability indicates the use of diverse clay sources and production techniques, reflecting hybrid technological practices shaped by cultural interaction and provisioning constraints. These results contribute to ongoing research within the CERIBAM (Iberian Atlantic Expansion in North Africa and Macaronesia) and Palarq-funded projects, which aim to reconstruct early colonial ceramic networks and sociotechnical dynamics. By integrating archaeometric data with archaeological and historical perspectives, this study aims to demonstrate the utility of non-invasive analytical protocols for understanding ceramic technology, intercultural exchange, and Atlantic material connectivity in early Creole formations while preserving the integrity of the collections. Full article