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Search Results (2,158)

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Keywords = precision agriculture applications

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31 pages, 946 KB  
Article
Multimodal Deep Learning for Pest and Disease Recognition and Crop Growth Assessment in Open-Field Agricultural Environments
by Jiayu Xiang, Jianxiang Pan, Hanwen Zhang, Xuekun Liu, Boxiu Liu, Jieling Tian and Shuo Yan
Agriculture 2026, 16(13), 1414; https://doi.org/10.3390/agriculture16131414 (registering DOI) - 29 Jun 2026
Abstract
Against the backdrop of the rapid development of smart agriculture, pest and disease monitoring and crop growth assessment for large-scale farmlands are of substantial importance for precision management and risk early warning. However, traditional unimodal visual methods are highly susceptible to illumination variation, [...] Read more.
Against the backdrop of the rapid development of smart agriculture, pest and disease monitoring and crop growth assessment for large-scale farmlands are of substantial importance for precision management and risk early warning. However, traditional unimodal visual methods are highly susceptible to illumination variation, canopy occlusion, scale differences, and background interference in real field environments, and thus fail to make full use of environmental sensing information and spatial priors. To address these issues, a multimodal target perception framework for intelligent farmland inspection is proposed in this study. By jointly integrating UAV imagery, time-series data from ground Internet of Things sensors, and spatial positional information, joint modeling of pest and disease recognition and crop growth assessment is achieved through cross-modal alignment and collaborative encoding, multi-scale target perception, and dynamic multimodal fusion and decision-making. Experimental results demonstrate that, in the pest and disease recognition task, the proposed method achieved a Precision of 91.63%, a Recall of 90.27%, an F1-score of 90.94%, and an mAP of 93.15%, significantly outperforming comparison models such as Faster R-CNN with ResNet50 backbone, YOLOv8-m, Swin Transformer-Tiny, and Multimodal Transformer. In the crop growth assessment task, an Accuracy of 89.96%, a Precision of 89.11%, a Recall of 88.74%, and a Macro-F1 of 88.92% were achieved, again clearly exceeding those of ResNet50, EfficientNet-B3, ViT-B/16, and conventional multimodal fusion models. The ablation study further verified the effectiveness of the cross-modal alignment module, the multi-scale target perception module, and the dynamic fusion module, with the complete model reaching 90.94%, 93.15%, and 88.92% in Pest F1, Pest mAP, and Growth Macro-F1, respectively. Furthermore, the net economic return regression experiment at the unit-area level further demonstrates that the proposed method can effectively connect state information with economic outcomes, showing strong application potential in return prediction, performance evaluation, and resource allocation optimization. These findings indicate that the proposed method can effectively improve perception accuracy and robustness in complex farmland environments, thereby providing reliable technical support for intelligent inspection, pest and disease early warning, and precision management in agricultural scenarios. Full article
(This article belongs to the Section Artificial Intelligence and Digital Agriculture)
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29 pages, 1919 KB  
Review
AI and IoT in Sugar Beet Systems: A Review of Monitoring, VOC Sensing, and Post-Harvest Applications
by Bakht Alam Khan and Sulaymon Eshkabilov
Sensors 2026, 26(13), 4072; https://doi.org/10.3390/s26134072 (registering DOI) - 26 Jun 2026
Viewed by 155
Abstract
The global sugar industry is facing increasing challenges due to climate variability, sustainability requirements, and the need for improved operational efficiency. These pressures are driving the search for advanced technological solutions to enhance productivity and resource management. Artificial intelligence (AI) has already demonstrated [...] Read more.
The global sugar industry is facing increasing challenges due to climate variability, sustainability requirements, and the need for improved operational efficiency. These pressures are driving the search for advanced technological solutions to enhance productivity and resource management. Artificial intelligence (AI) has already demonstrated significant potential across various agricultural sectors; however, a comprehensive evaluation of AI applications across the entire sugar industry value chain from crop cultivation to industrial processing and supply chain management remains limited. This review provides a detailed assessment of the current state of AI and internet of things (IoT) implementation in the sugar beet industry. It examines key applications, including precision agriculture for sugarcane and sugar beet cultivation, intelligent monitoring systems for early disease detection, and AI-driven decision support tools for resource optimization. In addition, the study explores the role of AI in sugar manufacturing processes, where machine learning and data-driven models are used to optimize milling operations, improve product quality control, and enable predictive maintenance of industrial equipment. AI technologies are also shown to enhance supply chain efficiency through improved demand forecasting, logistics optimization, and real-time data analytics. Monitoring volatile organic compounds (VOCs) is becoming increasingly important in sugar beet and sugarcane storage. Microbial activity during storage and fermentation can release VOCs such as ethanol, which act as early indicators of crop degradation and spoilage. Detecting these gases using modern gas sensors enables continuous monitoring of storage conditions and crop health. When sensor data is integrated with AI and IoT systems, it can be analyzed in real time to identify early signs of microbial activity, improve storage management, and optimize processing decisions. Such intelligent monitoring systems have the potential to reduce losses and enhance overall efficiency in the sugar production chain. Full article
(This article belongs to the Special Issue AI, IoT and Smart Sensors for Precision Agriculture: 2nd Edition)
46 pages, 1335 KB  
Systematic Review
Applications of Artificial Intelligence in Soil Characterization and Agriculture: A Systematic Review of Techniques, Models, and Applications
by Cesar Augusto Navarro Rubio, Hugo Martínez Ángeles, Mario Trejo Perea, José Luis Reyes Araiza, Guillermo Ronquillo-Lomeli, Ivan Gonzalez-Garcia, Eusebio Ventura Ramos and José Gabriel Ríos Moreno
Agronomy 2026, 16(13), 1241; https://doi.org/10.3390/agronomy16131241 (registering DOI) - 26 Jun 2026
Viewed by 95
Abstract
Artificial Intelligence (AI) has become a key enabler in soil science and agriculture, supporting advanced modeling, monitoring, and decision-making processes. This systematic review synthesizes recent developments in AI-based soil characterization and agricultural applications, with emphasis on soil physicochemical properties, digital soil mapping, irrigation [...] Read more.
Artificial Intelligence (AI) has become a key enabler in soil science and agriculture, supporting advanced modeling, monitoring, and decision-making processes. This systematic review synthesizes recent developments in AI-based soil characterization and agricultural applications, with emphasis on soil physicochemical properties, digital soil mapping, irrigation management, and crop yield prediction. Following the PRISMA 2020 framework, a structured search of the Scopus database identified 196 eligible studies published between 2018 and 2026. The reviewed literature reveals a clear transition toward data-driven approaches, with machine learning and deep learning models dominating recent research. Random Forest, Support Vector Machines, gradient boosting methods, artificial neural networks, Convolutional Neural Networks, and Long Short-Term Memory architectures were the most frequently reported techniques. The primary data sources included in situ sensors, laboratory measurements, remote sensing imagery, and environmental covariates, often integrated through multi-source data fusion frameworks. The results indicate that tree-based ensemble models provide robust performance across diverse soil properties, whereas deep learning models are particularly effective for spatiotemporal prediction and remote sensing applications. AI-driven systems are increasingly used to support precision agriculture through irrigation optimization, crop yield forecasting, digital soil mapping, and soil health monitoring. However, challenges remain regarding data quality and availability, model transferability across regions, and the limited interpretability of complex models. The findings highlight current research trends, methodological challenges, and future opportunities for the development of reliable and scalable AI-driven soil and agricultural systems. Full article
27 pages, 3230 KB  
Review
The Need for Omics Studies in Chronic Kidney Disease of Unknown Etiology (CKDu): A Narrative Review and Perspective
by Carly S. Chesterman, Amy S. Li, Chi-Yun Chen, Matthew Gibb, Richard J. Johnson, Zhoumeng Lin and Jared M. Brown
Int. J. Mol. Sci. 2026, 27(13), 5766; https://doi.org/10.3390/ijms27135766 - 26 Jun 2026
Viewed by 193
Abstract
Chronic Kidney Disease of Unknown Etiology (CKDu) is an ongoing global health concern, particularly affecting agricultural communities in equatorial regions. Unlike traditional chronic kidney disease (CKD), CKDu occurs without common risk factors such as diabetes, hypertension, or kidney stones. Its etiology remains poorly [...] Read more.
Chronic Kidney Disease of Unknown Etiology (CKDu) is an ongoing global health concern, particularly affecting agricultural communities in equatorial regions. Unlike traditional chronic kidney disease (CKD), CKDu occurs without common risk factors such as diabetes, hypertension, or kidney stones. Its etiology remains poorly understood, with environmental exposures, occupational hazards, and genetic susceptibility proposed as contributing factors. Omic technologies including genomics, transcriptomics, proteomics, metabolomics, and exposomics offer promising avenues to elucidate CKDu pathogenesis by enabling comprehensive molecular profiling and identification of biomarkers. Recent genomic studies have explored single nucleotide polymorphisms (SNPs) linked to kidney injury susceptibility, while transcriptomic analyses have identified differential expression of genes involved in oxidative stress and tubular injury pathways. Proteomic investigations have revealed candidate urinary biomarkers such as heat shock proteins and inflammatory mediators, and metabolomic profiling has highlighted alterations in amino acid and energy metabolism in affected individuals. Exposomic approaches are beginning to characterize cumulative chemical exposures, including pesticides and heavy metals, in endemic regions. This narrative review synthesizes current evidence on the application of omics approaches in CKDu research, highlights knowledge gaps, and proposes future directions for integrating multi-omics studies with machine learning and artificial intelligence approaches. Advancing omics-based investigations may provide critical insights into disease mechanisms, improve diagnostic precision, and inform targeted interventions for vulnerable populations. Full article
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23 pages, 2788 KB  
Review
Volume Estimation of Agricultural Products Using 2D Images: From Laboratory to Orchard
by Quan Wei, Danying Lei, Ziwei Song, Wei Zhao, Fakun Wei and Hua Yin
Horticulturae 2026, 12(7), 776; https://doi.org/10.3390/horticulturae12070776 - 25 Jun 2026
Viewed by 371
Abstract
Accurate and non-destructive volume estimation of agricultural products is essential for precision agriculture, yet remains challenging when transitioning from controlled laboratory conditions to complex orchard environments. Although 2D image-based volume estimation methods provide a cost-effective and scalable solution, existing studies are fragmented and [...] Read more.
Accurate and non-destructive volume estimation of agricultural products is essential for precision agriculture, yet remains challenging when transitioning from controlled laboratory conditions to complex orchard environments. Although 2D image-based volume estimation methods provide a cost-effective and scalable solution, existing studies are fragmented and lack a unified perspective on their real-world applicability. This review presents a systematic synthesis of 2D image-based volume estimation methods, explicitly framed through the laboratory-to-orchard transition. We categorized existing volume estimation approaches according to the sensing modality into monocular RGB-based approaches and depth-assisted methods, and further reviewed them based on the image processing methods. A key finding is that high-precision geometric estimation can be achieved in laboratory environments, whereas deep learning and RGB-D fusion have driven a shift from conventional geometric modeling toward data-driven and hybrid learning frameworks in orchard settings. However, 2D image-based volume estimation remains fundamentally limited by scale ambiguity, severe occlusion, and sensitivity to illumination and background variability in real orchard environment. Overall, this review provides a unified perspective for understanding volume estimation methodology across environments and offers guidance for developing robust, scalable, and field-deployable volume estimation systems for real-world agricultural applications. Full article
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38 pages, 1879 KB  
Systematic Review
Precision Livestock Farming and Biomedical Engineering: pAssessing Feed Quality, Animal Health, and Behavior Using Machine Learning for Sensor Data
by Nikolay Kiktev, Danylo Hradoboiev, Mykola Pravilov, Ievgen Antypov, Yuliia Meish, Liliia Stroianovska, Pawel Kielbasa and Taras Hutsol
Sensors 2026, 26(13), 4015; https://doi.org/10.3390/s26134015 - 24 Jun 2026
Viewed by 213
Abstract
This review analyses and logically structures modern intelligent sensor technologies in the context of animal husbandry, feed production, and veterinary medicine. The main research discussed in the article focuses on machine learning based on modern neural network models, computer vision, and sensor systems [...] Read more.
This review analyses and logically structures modern intelligent sensor technologies in the context of animal husbandry, feed production, and veterinary medicine. The main research discussed in the article focuses on machine learning based on modern neural network models, computer vision, and sensor systems that are transforming the methods for assessing the health, behavior, and nutrition of farm animals. The first part examines modern approaches to quality control and optimization of mineral and vitamin premixes, including visual inspection using visual sensors and neural networks. Key roles are played by precise dosing, component stability (minerals, vitamins), and the transition to more bioefficient organic forms of micronutrients to reduce environmental impact. Improvements in feed and premix production are analyzed, including automation, energy management, and the use of machine learning for non-destructive quality control, defect detection, mixing homogeneity assessment, and vitamin stability prediction. The second part analyzes methods for animal location and behavior detection. This article presents computer vision-based systems, including modifications of YOLO, for automatically tracking and classifying key behavioral patterns (lying down, standing, feeding, and aggression) in cattle and pigs, even in crowded conditions. It also discusses the use of ultra-wideband (UWB) systems and accelerometers combined with machine learning for high-precision positioning and detection of specific behavioral anomalies, such as lameness and playfulness. The third section focuses on the application of machine learning in veterinary diagnostics, including the automated interpretation of medical images (X-ray, ultrasound, and MRI) as sensor data streams for the diagnosis of cardiovascular, oncological, and orthopedic diseases in farm and small animals. Furthermore, the article examines the use of machine learning models for proactive disease diagnosis in farm animals and poultry based on multimodal data and image analysis. Considerable attention is given to methods and tools for radiometric diagnosis of animal diseases at an early stage using microwave sensors, as well as laser therapy and surgery in veterinary medicine. The review concludes that the integration of intelligent systems enables a transition to data-driven livestock management, significantly improving animal welfare and, consequently, the efficiency and sustainability of agricultural production. Full article
(This article belongs to the Section Smart Agriculture)
19 pages, 3974 KB  
Systematic Review
Impact of Organic Fertilizer Substitution on Greenhouse Gas Emissions from Vegetable Production Systems: A Global Meta-Analysis
by Lusheng Li, Xiangjie Chen, Lili Zhao, Ling Zhong, Lixia Guo, Yuan Wang, Hongbo Xue, Haixia Qin, Minggui Zhang and Guanghua Yao
Agronomy 2026, 16(12), 1205; https://doi.org/10.3390/agronomy16121205 - 21 Jun 2026
Viewed by 227
Abstract
Controversy persists on a global scale regarding the trade-offs between greenhouse gas (GHG) emissions, yield, the global warming potential (GWP), and GHG intensity (GHGI) following organic fertilizer substitution within vegetable cropping systems. This study aimed to quantify these effects under diverse conditions and [...] Read more.
Controversy persists on a global scale regarding the trade-offs between greenhouse gas (GHG) emissions, yield, the global warming potential (GWP), and GHG intensity (GHGI) following organic fertilizer substitution within vegetable cropping systems. This study aimed to quantify these effects under diverse conditions and elucidate the direct and indirect drivers governing these outcomes through a meta-analysis and structural equation modeling (SEM). We synthesized 655 paired observations from 69 published studies using random-effects meta-analysis, finding that organic fertilizer substitution significantly increased CH4 emissions and GWP compared to inorganic fertilizer controls. Although this was the general trend, organic fertilizer could reduce GWP under specific climatic and soil conditions by reducing N2O emissions, such as mean annual precipitation <400 mm or soil total nitrogen ≥3 g kg−1. These conditions were also associated with substantially higher yield and lower GHGI. Furthermore, SEM demonstrated that field management practices exerted significant direct effects on N2O emissions, GWP, and GHGI. Reductions in N2O emissions, GWP, and GHGI could be achieved with fertilizer application duration ≥10 years, total N application rate ≥300 kg ha−1, and field cultivation or plowing. GHGI was also reduced through yield enhancement under a moderate organic substitution rate (33–66%) or irrigation ≥300 mm. Our study provides a scientific basis for moving beyond universal recommendations towards precision organic management, which is essential for optimizing fertilization strategies to mitigate agricultural GHG emissions. Full article
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27 pages, 9358 KB  
Review
Selenium in Plants from Mechanisms to Research Frontiers: A Mini-Review and Bibliometric Analysis from 2000 to 2025
by Haibo Wang, Zhikang Guo, Fang Chen, Yunan Liu and Mu Peng
Agronomy 2026, 16(12), 1204; https://doi.org/10.3390/agronomy16121204 - 21 Jun 2026
Viewed by 317
Abstract
Selenium (Se) is a beneficial element involved in plant growth, metabolism, stress adaptation, and crop quality improvement, but its effects are strongly influenced by chemical form, application dose, plant species, growth stage, and environmental conditions. To integrate mechanistic understanding with global research trends, [...] Read more.
Selenium (Se) is a beneficial element involved in plant growth, metabolism, stress adaptation, and crop quality improvement, but its effects are strongly influenced by chemical form, application dose, plant species, growth stage, and environmental conditions. To integrate mechanistic understanding with global research trends, this study combines a concise mini-review with a bibliometric analysis of Se research in plants from 2000 to 2025. The mini-review summarizes Se speciation and bioavailability in the soil–plant–microbe system, root uptake and long-distance transport, metabolic assimilation and detoxification, physiological regulation, stress tolerance, biofortification, and nano-Se applications. Bibliographic data were retrieved from the Web of Science Core Collection and analyzed using CiteSpace, VOSviewer, and Scimago Graphica. A total of 3451 valid publications were identified, showing a sustained increase in annual output, especially after 2018. The field has expanded from early studies on Se speciation, uptake, assimilation, and antioxidant responses toward broader themes involving crop biofortification, molecular regulation, stress physiology, foliar application, nano-Se applications, green synthesis, and phytoremediation. Overall, plant Se research has evolved into an interdisciplinary field linking mechanistic studies with safe agricultural application. Future work should emphasize standardized experimental frameworks, causal mechanism validation, precise biofortification, field-based evaluation, and safety assessment of emerging Se-based technologies. Full article
(This article belongs to the Special Issue Nutrient Enrichment and Crop Quality in Sustainable Agriculture)
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33 pages, 4922 KB  
Review
Agricultural Variable-Rate Nozzles: A Review of Technologies and Control Approaches
by Mengmeng Niu, Qingyi Zhang, Peng Qi, Xinzhong Wang, Rodrigo Quintana, Huimin Fang, Zhiming Wei, Zhihao Gong and Shicheng Wang
Agronomy 2026, 16(12), 1203; https://doi.org/10.3390/agronomy16121203 - 20 Jun 2026
Viewed by 179
Abstract
As the core actuation component of intelligent precision spraying systems, the variable-rate nozzle is essential for achieving on-demand agricultural spraying; improving the use efficiency of water, fertilizers and pesticides; and reducing environmental pollution. This paper systematically reviews the development of agricultural variable-rate nozzles, [...] Read more.
As the core actuation component of intelligent precision spraying systems, the variable-rate nozzle is essential for achieving on-demand agricultural spraying; improving the use efficiency of water, fertilizers and pesticides; and reducing environmental pollution. This paper systematically reviews the development of agricultural variable-rate nozzles, from early mechanical profiling structures to modern intelligent control technologies based on Pulse Width Modulation (PWM). First, the existing variable-rate nozzles are classified into three major categories: electromagnetic-integrated type, centrifugal type, and variable-diameter type. A comparative analysis is conducted from three dimensions of working principle, performance characteristics and application scenarios, to delineate the respective advantages and limitations of each nozzle category. Second, the paper examines key technological advances in three areas: high-frequency solenoid valves, PWM control, and pressure and flow stabilization. It identifies the nonlinear response of solenoid valves, flow distortion under low duty cycles, and water hammer pressure fluctuation induced by high-speed switching as the three core technical bottlenecks at the current stage. Subsequently, the latest achievements and typical methodologies of variable-rate nozzles in structural design, simulation and experimental analysis are systematically reviewed, and their application performance in scenarios including field crops, orchards, protected agriculture and beyond are summarized. Finally, the remaining open issues in this field are put forward. It is suggested that future research should focus on key breakthroughs in the development of corrosion and wear-resistant high-frequency solenoid valves, the formation mechanism and suppression methods of pressure fluctuation, as well as adaptive algorithms based on machine learning or Model Predictive Control (MPC), to promote the leapfrog development of agricultural variable-rate nozzle technology from single variable control to multi-factor coupling optimization. All references cited in this paper are from articles published after the year 2000. Among them, the literature published in the last decade accounts for 86.6%, and literature published in the last five years accounts for 58.9%. Full article
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19 pages, 1663 KB  
Review
Challenges and Development Trends of Crop–Hydro Digital Twin Technology
by Shihan Wang, Jiaqing He, Aidi Huo, Yapeng Li, Yibing Cao, Salah Elsayed and Jahangir Muhammad Ilyas
Water 2026, 18(12), 1516; https://doi.org/10.3390/w18121516 - 19 Jun 2026
Viewed by 489
Abstract
Under the dual constraints of global food security and ecological protection, conventional agriculture is hampered by low resource efficiency and sluggish environmental response. Crop digital twin technology establishes a dynamic virtual reality system that integrates crops, environment, and water to enable real-time interaction [...] Read more.
Under the dual constraints of global food security and ecological protection, conventional agriculture is hampered by low resource efficiency and sluggish environmental response. Crop digital twin technology establishes a dynamic virtual reality system that integrates crops, environment, and water to enable real-time interaction and optimization. Based on the existing literature, this paper reviews the concept, architecture, and core modules of this technology and summarizes its applications in precision irrigation and crop monitoring. There are three major bottlenecks that persist, including limited high-frequency multi-source sensing and spatiotemporal fusion, insufficient parameter calibration and dynamic updating, and weak cross-scale integration from plant to watershed. Water is increasingly recognized as the key constraint and control variable and acting as both the central physiological driver of crop growth and the mass-flow link that connects the soil–plant–atmosphere continuum. The spatiotemporal dynamics of crop water deficit, compensatory root water uptake, evapotranspiration feedback, and the hydraulic behavior of irrigation-district canal systems constitute the core hydrological processes that must be simulated within the digital twin. Synchronizing crop water demand, soil moisture dynamics, atmospheric evapotranspiration, and irrigation scheduling within a unified spatiotemporal framework establishes a complete sensing, diagnosis, prediction and regulation technical chain. This chain offers a core pathway for alleviating agricultural water scarcity, improving irrigation efficiency, and ensuring food security. Full article
(This article belongs to the Special Issue Application of Water-Saving Irrigation in Agricultural Development)
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30 pages, 86356 KB  
Article
Geometric Principles of Stereo Vision: A Quantitative Evaluation and Physical Validation of the Classical Pipeline
by Angel Fernando Ceballos-Espinoza, David Balderas-Silva, Alfredo Diaz-Lara and Rita Q. Fuentes-Aguilar
Appl. Sci. 2026, 16(12), 6212; https://doi.org/10.3390/app16126212 - 19 Jun 2026
Viewed by 150
Abstract
Stereo vision is essential for passive three-dimensional perception in resource-constrained applications that require low power consumption, predictable latency, and explainable geometry. Although deep learning architectures dominate recent benchmarks, the classical block-matching pipeline remains a foundational approach. Optimizing this pipeline involves navigating complex trade-offs [...] Read more.
Stereo vision is essential for passive three-dimensional perception in resource-constrained applications that require low power consumption, predictable latency, and explainable geometry. Although deep learning architectures dominate recent benchmarks, the classical block-matching pipeline remains a foundational approach. Optimizing this pipeline involves navigating complex trade-offs among matching robustness, map density, and computational efficiency. This study systematically surveys and physically validates the classical stereo framework. After revisiting geometric first principles, three matching costs (SAD, NCC, ZNCC) are benchmarked alongside Sobel preprocessing and structural refinements, with subsequent validation using a calibrated consumer webcam rig. Middlebury benchmarks (2001–2021) indicate that while SAD fails under complex radiometric distortion, NCC consistently achieves superior quantitative metrics, incurring only a 1.2-fold computational overhead. Extending the disparity search range improves foreground localization, while block size imposes a trade-off between resolving the aperture problem and preserving fine geometric detail. To bridge theoretical analysis and practical deployment, the pipeline is validated using a custom-calibrated consumer stereo rig. The optimized Sobel-NCC architecture is then evaluated for real-time edge deployment on constrained hardware (NVIDIA Jetson Nano) and narrow-baseline sensors (OAK-D SR) in the context of agricultural robotic manipulation. By prioritizing metric precision over dense prediction, the classical pipeline reconstructs target surfaces with approximately 1 cm depth accuracy at 21 frames per second. These results demonstrate that optimized local algorithms offer deterministic and reliable geometric foundations for real-time edge-computed robotics. Although neural networks are essential for dense reconstructions in ill-posed regions, the foundational principles established here remain indispensable for advanced stereo vision system deployment. Full article
(This article belongs to the Section Robotics and Automation)
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23 pages, 10456 KB  
Article
An Attention-Based Deep Learning Framework for Detecting Water Stress in Basil (Ocimum basilicum L.) Plants
by Oğuzhan Kilim, Tuncay Yiğit and Hamit Armağan
Appl. Sci. 2026, 16(12), 6192; https://doi.org/10.3390/app16126192 - 18 Jun 2026
Viewed by 182
Abstract
With the occurrence of global climate change and the depletion of agricultural water resources, there is a growing need to develop rapid, non-destructive, and autonomous plant health monitoring systems. As an economically valuable crop, Ocimum basilicum L. (basil) is sensitive to changes in [...] Read more.
With the occurrence of global climate change and the depletion of agricultural water resources, there is a growing need to develop rapid, non-destructive, and autonomous plant health monitoring systems. As an economically valuable crop, Ocimum basilicum L. (basil) is sensitive to changes in water availability and may exhibit stress-related morphological variations under drought and over-irrigation conditions. However, due to the visual similarity of leaf symptoms under drought stress, waterlogging stress, and optimal irrigation conditions, accurately distinguishing these conditions remains challenging in practical applications. To address this challenge, this paper presents an attention-based dual-branch deep learning framework designed to extract both subtle leaf details and channel-related features from high-resolution plant images. By combining the Convolutional Block Attention Module (CBAM) and Squeeze-and-Excitation (SE) mechanism in a parallel structure, the proposed network improves the analysis of high-resolution images with an input size of 720 × 720 pixels. Under controlled environmental conditions, with ground-truth labels obtained using soil moisture sensor measurements, the proposed model was compared with eight deep learning architectures, including DenseNet121, InceptionV3, and VGG16. The proposed model achieved a hold-out evaluation accuracy of 99.54%, outperforming the second-best model, DenseNet121, which achieved 96.43%. In addition, the proposed model reached a class-specific precision value of 100% for the Drought Stress category and achieved an area under the receiver operating characteristic curve of 1.00 under the controlled experimental setting. Taylor Diagram analysis also indicated that the model closely preserved the variability pattern of the reference data. These results suggest that the proposed application-specific framework may support non-destructive basil water-stress detection under controlled conditions. After further validation with larger datasets, different cultivars, variable environmental conditions, and real-world agricultural scenarios, the proposed approach may contribute to precision irrigation management and sustainable agricultural production. The contribution of this study should be interpreted as an application-specific implementation and evaluation of complementary attention mechanisms for controlled-environment basil water-stress classification, rather than as the introduction of a fundamentally new deep learning methodology. Full article
(This article belongs to the Section Agricultural Science and Technology)
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32 pages, 23726 KB  
Review
Medicinal Plant-Derived Exosome-like Nanoparticles: From Basic Research to Biomedical Applications
by Huan Deng, Yi-Wen Zhang, Qian-Fu Zhao and Zhi-Jun Huang
Pharmaceutics 2026, 18(6), 750; https://doi.org/10.3390/pharmaceutics18060750 (registering DOI) - 18 Jun 2026
Viewed by 318
Abstract
Plant-derived exosome-like nanoparticles (PELNs), a subset of extracellular vesicle (EV) secreted by plant cells, have emerged as revolutionary biomaterial with broad applications in biomedicine, agriculture, and nanotechnology. Structurally, PELNs feature a phospholipid bilayer homologous to plant cell membranes, encapsulating bioactive components such as [...] Read more.
Plant-derived exosome-like nanoparticles (PELNs), a subset of extracellular vesicle (EV) secreted by plant cells, have emerged as revolutionary biomaterial with broad applications in biomedicine, agriculture, and nanotechnology. Structurally, PELNs feature a phospholipid bilayer homologous to plant cell membranes, encapsulating bioactive components such as proteins, nucleic acids, lipids, and secondary metabolites. The native structure of PELNs endows them with enhanced bioavailability, reduced immunogenicity, and improved barrier penetration for precise tissue delivery. Recent studies highlight the cross-kingdom therapeutic potential of PELNs in mammals, including antitumor, anti-inflammatory, tissue repair, immunomodulation and so on. This review comprehensively summarized recent advancements in PELN research, including innovative isolation techniques, molecular characterization, their roles in drug delivery and disease therapy. We also discussed challenges in standardization, scalability, and regulatory frameworks which could provide future perspectives for translating PELNs into clinical and industrial applications. Full article
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24 pages, 4006 KB  
Article
Benchmarking Landsat-8 Collection 2 Level-2 Land Surface Temperature Accuracy Using SURFRAD Stations: Effects of Seasonality and Atmospheric Water Vapor
by Almustafa AbdElkader Ayek, Mohannad Ali Loho, Nasser Ibrahem, Afnan Abdullah Alturki, Youssef M. Youssef and Mayada Abdelkader Abdelaziz
Atmosphere 2026, 17(6), 615; https://doi.org/10.3390/atmos17060615 - 18 Jun 2026
Viewed by 403
Abstract
Land Surface Temperature (LST) is essential for climate monitoring, drought assessment, and urban heat analysis. Despite its importance, the Landsat-8 Collection 2 Level-2 (C2L2) LST product has not been rigorously validated using ground measurements—a critical gap this study addresses. We present the first [...] Read more.
Land Surface Temperature (LST) is essential for climate monitoring, drought assessment, and urban heat analysis. Despite its importance, the Landsat-8 Collection 2 Level-2 (C2L2) LST product has not been rigorously validated using ground measurements—a critical gap this study addresses. We present the first comprehensive accuracy assessment using 382 coincident satellite–ground observations collected from seven Surface Radiation Budget Network (SURFRAD) stations distributed across diverse climatic regions of the United States during the period 2023–2025. The validation results indicate strong overall agreement between satellite-derived and ground-measured temperatures, yielding an RMSE of 4.20 °C, a coefficient of determination (R2) of 0.91, and a Pearson correlation coefficient (r) of 0.98. These statistics demonstrate the high reliability of the C2L2 LST product across a wide range of environmental conditions. Nevertheless, a systematic warm bias of 1.75 °C was observed, indicating a tendency toward temperature overestimation. Model performance exhibited pronounced seasonal variability. The highest accuracy was achieved during winter conditions (RMSE = 2.17 °C; r = 0.99), whereas performance declined considerably during summer months (RMSE = 5.84 °C; r = 0.91). Analysis of atmospheric water vapor content revealed significant associations with retrieval errors at high-elevation and arid locations, particularly at FPK (r = 0.78) and DRA (r = 0.75), based on 106 matched observations. These relationships provide important insight into the atmospheric factors contributing to seasonal variations in retrieval accuracy. Temperature-dependent analyses further demonstrated that retrieval uncertainty increases with surface temperature. Performance progressively deteriorated from cooler to warmer thermal regimes, with RMSE values increasing from approximately 2.05 °C for temperatures below 20 °C to 5.71 °C for temperatures exceeding 40 °C. Spatial evaluation also revealed substantial differences among stations. Relatively homogeneous, low-elevation sites exhibited superior performance (GWN: RMSE = 2.60 °C; SXF: RMSE = 2.55 °C), whereas stations located in mountainous or topographically complex environments showed reduced accuracy (TBL: RMSE = 5.14 °C; FPK: RMSE = 5.62 °C). These outcomes emphasize the influence of terrain complexity and atmospheric heterogeneity on LST retrieval performance. Overall, this study establishes the first comprehensive benchmark for evaluating the reliability of Landsat-8 C2L2 LST products. The results provide valuable guidance for their application in climate research, precision agriculture, hydrological modeling, and environmental monitoring. Furthermore, the findings identify specific environmental conditions requiring enhanced validation efforts and suggest opportunities for future algorithm refinement through improved atmospheric correction procedures and more accurate surface emissivity characterization. Full article
(This article belongs to the Section Atmospheric Techniques, Instruments, and Modeling)
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30 pages, 43374 KB  
Article
Evaluating the Potential of Unmanned Aerial Vehicle-Derived Data for Evapotranspiration Estimation in Smallholder Farms
by Ameera Yacoob, Shaeden Gokool, Alistair Clulow, Maqsooda Mahomed, Vivek Naiken and Tafadzwanashe Mabhaudhi
Remote Sens. 2026, 18(12), 2027; https://doi.org/10.3390/rs18122027 - 18 Jun 2026
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Abstract
The rising global population has heightened food demand, placing pressure on agricultural systems, particularly in water-scarce regions such as South Africa. Smallholder farmers, essential to the sector, face climatic variability and resource constraints, necessitating innovative solutions to enhance sustainability and productivity. This study [...] Read more.
The rising global population has heightened food demand, placing pressure on agricultural systems, particularly in water-scarce regions such as South Africa. Smallholder farmers, essential to the sector, face climatic variability and resource constraints, necessitating innovative solutions to enhance sustainability and productivity. This study evaluates unmanned aerial vehicles (UAVs) for generating spatially explicit evapotranspiration (ET) estimates in a small-scale sugarcane field, supporting precision water management. Vegetation indices (VIs) derived from UAV-based multispectral imagery were used to predict actual ET (ETa) and validated against eddy covariance measurements. Five models were assessed, including Normalised Difference Vegetation Index (NDVI)-based and Enhanced Vegetation Index (EVI)-based approaches. Machine learning was used to relate crop coefficients (Kc) to NDVI, enabling improved estimation. The two-band EVI (EVI2) model achieved the highest accuracy, with an R2 of 0.63, an RMSE of 0.67, and an MAE of 0.52. ET-VI approaches, particularly EVI2, require lower data and technical complexity, making them suitable for smallholder systems. However, reducing dependence on in situ data remains essential to improve accessibility of remote sensing approaches for agricultural water management in resource-limited environments. These findings demonstrate the potential of UAV-based ETa modelling to support field-scale irrigation decision-making while highlighting the need for further refinement to improve operational applicability across diverse smallholder farming contexts and beyond. Full article
(This article belongs to the Special Issue Near Real-Time (NRT) Agriculture Monitoring)
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