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13 Results Found

  • Article
  • Open Access
25 Citations
9,274 Views
20 Pages

Quantification of Polyfunctional Thiols in Wine by HS-SPME-GC-MS Following Extractive Alkylation

  • Lauren E. Musumeci,
  • Imelda Ryona,
  • Bruce S. Pan,
  • Natalia Loscos,
  • Hui Feng,
  • Michael T. Cleary and
  • Gavin L. Sacks

6 July 2015

Analyses of key odorous polyfunctional volatile thiols in wines (3-mercaptohexanol (3-MH), 3-mercaptohexylacetate (3-MHA), and 4-mercapto-4-methyl-2-pentanone (4-MMP)) are challenging due to their high reactivity and ultra-trace concentrations, espe...

  • Article
  • Open Access
10 Citations
2,977 Views
12 Pages

At present, non-alcoholic and low-alcoholic beers (NABLABs), in addition to their premature sensitivity to oxidation, still suffer from a lack of fruity fermentation aromas. Maltose/maltotriose-negative yeasts offer a highly attractive alternative fo...

  • Article
  • Open Access
4 Citations
1,960 Views
11 Pages

15 January 2025

The prevalence of glutathionylated (G-) precursors of polyfunctional thiols (PFTs) over their free forms has prompted investigating how to optimize the enzymatic breakdown of these precursors with yeast during lager, ale, and non-alcoholic/low-alcoho...

  • Article
  • Open Access
2 Citations
1,095 Views
12 Pages

Two enzymatic assays, based on release of p-nitroaniline and its spectrophotometric detection at 405 nm, were used to screen lager and ale brewing yeasts for carboxypeptidase and γ-glutamyltranspeptidase activity. Both activities were found in...

  • Article
  • Open Access
16 Citations
11,683 Views
15 Pages

The Sensorial and Chemical Changes in Beer Brewed with Yeast Genetically Modified to Release Polyfunctional Thiols from Malt and Hops

  • Richard W. Molitor,
  • Jeremy I. Roop,
  • Charles M. Denby,
  • Charles J. Depew,
  • Daniel S. Liu,
  • Sara E. Stadulis and
  • Thomas H. Shellhammer

The biotransformation of hop aroma, particularly by the cysteine S-conjugate beta-lyase enzyme (CSL), has been a recent topic of tremendous interest among brewing scientists and within the brewing community. During a process often referred to as biot...

  • Article
  • Open Access
1 Citations
1,329 Views
20 Pages

Exploring the Influence of Different Saccharomyces cerevisiae Strains and Hop Varieties on Beer Composition and Sensory Profiles

  • Antonella Costantini,
  • Maurizio Petrozziello,
  • Christos Tsolakis,
  • Andriani Asproudi,
  • Enrico Vaudano,
  • Laura Pulcini,
  • Federica Bonello,
  • Katya Carbone and
  • Maria Carla Cravero

2 July 2025

The influence of different Saccharomyces cerevisiae (Sc) strains and hop varieties on the physical, chemical and sensory properties of beer was investigated. ISE77, an oenological Sc strain screened for the IRC7 gene and β-lyase activity, and a...

  • Review
  • Open Access
8 Citations
3,493 Views
19 Pages

11 July 2022

Volatile polyfunctional thiol compounds, particularly 3-sulfanylhexan-1-ol (3SH) and 3-sulfanylhexyl acetate (3SHA), are key odorants contributing to the aroma profile of many wine styles, generally imparting tropical grapefruit and passionfruit arom...

  • Article
  • Open Access
8 Citations
3,566 Views
17 Pages

22 March 2023

A series of poly(thiourethanes) (PTUs) from biobased monomers have been synthesized. Limonene and squalene were transformed into polyfunctional thiols by thiol-ene reaction with thioacetic acid and further saponification. They were then reacted in di...

  • Feature Paper
  • Article
  • Open Access
12 Citations
5,401 Views
19 Pages

Modulation of Volatile Thiol Release during Fermentation of Red Musts by Wine Yeast

  • Antonio G. Cordente,
  • Christopher D. Curtin,
  • Mark Solomon,
  • Allie C. Kulcsar,
  • Flynn Watson,
  • Lisa Pisaniello,
  • Simon A. Schmidt and
  • Damian Espinase Nandorfy

2 March 2022

During the alcoholic fermentation of grape sugars, wine yeast produces a range of secondary metabolites that play a critical role in the aroma profile of wines. One of the most impactful yeast-modified compound families, particularly in white wines,...

  • Review
  • Open Access
10 Citations
3,956 Views
15 Pages

Microbial β C-S Lyases: Enzymes with Multifaceted Roles in Flavor Generation

  • Mathieu Schwartz,
  • Nicolas Poirier,
  • Jade Moreno,
  • Alena Proskura,
  • Mélanie Lelièvre,
  • Jean-Marie Heydel and
  • Fabrice Neiers

β C-S lyases (β-CSLs; EC 4.4.1.8) are enzymes catalyzing the dissociation of β carbon–sulfur bonds of cysteine S-conjugates to produce odorant metabolites with a free thiol group. These enzymes are increasingly studied for their...

  • Article
  • Open Access
6 Citations
1,746 Views
17 Pages

Even when fresh, non-alcoholic, and low-alcoholic beers (NABLABs) exhibit significant staling defects due to premature oxidation. In this study, the antioxidant power of eleven fresh commercial NABLABs was assessed by means of three different assays:...

  • Review
  • Open Access
22 Citations
12,132 Views
16 Pages

A great deal of research in the alcoholic beverage industry was done on non-Saccharomyces yeast strains in recent years. The increase in research interest could be attributed to the changing of consumer tastes and the search for new beer sensory expe...

  • Article
  • Open Access
1 Citations
2,469 Views
15 Pages

Protease and DNase Activities of a Very Stable High-Molecular-Mass Multiprotein Complex from Sea Cucumber Eupentacta fraudatrix

  • Anna M. Timofeeva,
  • Irina A. Kostrikina,
  • Pavel S. Dmitrenok,
  • Svetlana E. Soboleva and
  • Georgy A. Nevinsky

Only some human organs, including the liver, are capable of very weak self-regeneration. Some marine echinoderms are very useful for studying the self-regeneration processes of organs and tissues. For example, sea cucumbers Eupentacta fraudatrix (hol...