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Search Results (3,950)

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Keywords = pollution transport

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22 pages, 1814 KiB  
Article
Integrating Environmental Sensing into Cargo Bikes for Pollution-Aware Logistics in Last-Mile Deliveries
by Leonardo Cameli, Margherita Pazzini, Riccardo Ceriani, Valeria Vignali, Andrea Simone and Claudio Lantieri
Sensors 2025, 25(15), 4874; https://doi.org/10.3390/s25154874 (registering DOI) - 7 Aug 2025
Abstract
Cycling represents a significant share of urban transportation, especially in terms of last-mile delivery. It has clear benefits for delivery times, as well as for environmental issues related to freight distribution. Furthermore, the increasing accessibility of low-cost environmental sensors (LCSs) provides an opportunity [...] Read more.
Cycling represents a significant share of urban transportation, especially in terms of last-mile delivery. It has clear benefits for delivery times, as well as for environmental issues related to freight distribution. Furthermore, the increasing accessibility of low-cost environmental sensors (LCSs) provides an opportunity for urban monitoring in any situation. Moving in this direction, this research aims to investigate the use of LCSs to monitor particulate PM2.5 and PM10 levels and map them over delivery ride paths. The calibration process took 49 days of measurements into account, spanning different seasonal conditions (from May 2024 to November 2024). The employment of multiple linear regression and robust regression revealed a strong correlation between pollutant levels from two sources and other factors such as temperature and humidity. Subsequently, a one-month trial was carried out in the city of Faenza (Italy). In this study, a commercially available LCS was mounted on a cargo bike for measurement during delivery processes. This approach was adopted to reveal biker exposure to air pollutants. In this way, the operator’s route would be designed to select the best route in terms of delivery timing and polluting factors in the future. Furthermore, the integration of environmental monitoring to map urban environments has the potential to enhance the accuracy of local pollution mapping, thereby supporting municipal efforts to inform citizens and develop targeted air quality strategies. Full article
(This article belongs to the Section Environmental Sensing)
15 pages, 5141 KiB  
Article
Efficient Copper Biosorption by Rossellomorea sp. ZC255: Strain Characterization, Kinetic–Equilibrium Analysis, and Genomic Perspectives
by Hao-Tong Han, Han-Sheng Zhu, Jin-Tao Zhang, Xin-Yun Tan, Yan-Xin Wu, Chang Liu, Xin-Yu Liu and Meng-Qi Ye
Microorganisms 2025, 13(8), 1839; https://doi.org/10.3390/microorganisms13081839 - 7 Aug 2025
Abstract
Heavy metal pollution, particularly copper contamination, threatens the ecological environment and human survival. In response to this pressing environmental issue, the development of innovative remediation strategies has become imperative. Bioremediation technology is characterized by remarkable advantages, including its ecological friendliness, cost-effectiveness, and operational [...] Read more.
Heavy metal pollution, particularly copper contamination, threatens the ecological environment and human survival. In response to this pressing environmental issue, the development of innovative remediation strategies has become imperative. Bioremediation technology is characterized by remarkable advantages, including its ecological friendliness, cost-effectiveness, and operational efficiency. In our previous research, Rossellomorea sp. ZC255 demonstrated substantial potential for environmental bioremediation applications. This study investigated the removal characteristics and underlying mechanism of strain ZC255 and revealed that the maximum removal capacity was 253.4 mg/g biomass under the optimal conditions (pH 7.0, 28 °C, and 2% inoculum). The assessment of the biosorption process followed pseudo-second-order kinetics, while the adsorption isotherm may fit well with both the Langmuir and Freundlich models. Cell surface alterations on the Cu(II)-treated biomass were observed through scanning electron microscopy (SEM). Cu(II) binding functional groups were determined via Fourier transform infrared spectroscopy (FTIR) analysis. Simultaneously, the genomic analysis of strain ZC255 identified multiple genes potentially involved in heavy metal resistance, transport, and metabolic processes. These studies highlight the significance of strain ZC255 in the context of environmental heavy metal bioremediation research and provide a basis for using strain ZC255 as a copper removal biosorbent. Full article
(This article belongs to the Section Environmental Microbiology)
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17 pages, 780 KiB  
Review
Progress in the Study of Plant Nitrogen and Potassium Nutrition and Their Interaction Mechanisms
by Weiyu Cao, Hai Sun, Cai Shao, Yue Wang, Jiapeng Zhu, Hongjie Long, Xiaomeng Geng and Yayu Zhang
Horticulturae 2025, 11(8), 930; https://doi.org/10.3390/horticulturae11080930 - 7 Aug 2025
Abstract
Nitrogen (N) and potassium (K) are essential macronutrients for plants whose functions and interactions profoundly influence plant physiological metabolism, environmental adaptation, and agricultural production efficiency. This review summarizes research advances in plant N and K nutrition and their interaction mechanisms, elucidating the key [...] Read more.
Nitrogen (N) and potassium (K) are essential macronutrients for plants whose functions and interactions profoundly influence plant physiological metabolism, environmental adaptation, and agricultural production efficiency. This review summarizes research advances in plant N and K nutrition and their interaction mechanisms, elucidating the key physiological functions of N and K individually and their respective absorption and transport mechanisms involving transporters such as NRTs and HAKs/KUPs. The review discusses the types of nutrient interactions (synergism and antagonism), with a primary focus on the physiological basis of N–K interactions and their interplay in root absorption and transport (e.g., K+-NO3 co-transport; NH4+ inhibition of K+ uptake), photosynthesis (jointly optimizing CO2 conductance, mesophyll conductance, and N allocation within photosynthetic machinery to enhance photosynthetic N use efficiency, PNUE), as well as sensing, signaling, co-regulation, and metabolism. This review emphasizes that N–K balance is crucial for improving crop yield and quality, enhancing fertilizer use efficiency (NUE/KUE), and reducing environmental pollution. Consequently, developing effective N–K management strategies based on these interaction mechanisms and implementing Balanced Fertilization Techniques (BFT) to optimize N–K ratios and application strategies in agricultural production represent vital pathways for ensuring food security, addressing resource constraints, and advancing green, low-carbon agriculture, including through coordinated management of greenhouse gas emissions. Full article
(This article belongs to the Section Plant Nutrition)
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28 pages, 1145 KiB  
Article
Uncovering Hidden Risks: Non-Targeted Screening and Health Risk Assessment of Aromatic Compounds in Summer Metro Carriages
by Han Wang, Guangming Li, Cuifen Dong, Youyan Chi, Kwok Wai Tham, Mengsi Deng and Chunhui Li
Buildings 2025, 15(15), 2761; https://doi.org/10.3390/buildings15152761 - 5 Aug 2025
Abstract
Metro carriages, as enclosed transport microenvironments, have been understudied regarding pollution characteristics and health risks from ACs, especially during high-temperature summers that amplify exposure. This study applied NTS techniques for the first time across three major Chengdu metro lines, systematically identifying sixteen ACs, [...] Read more.
Metro carriages, as enclosed transport microenvironments, have been understudied regarding pollution characteristics and health risks from ACs, especially during high-temperature summers that amplify exposure. This study applied NTS techniques for the first time across three major Chengdu metro lines, systematically identifying sixteen ACs, including hazardous species such as acetophenone, benzonitrile, and benzoic acid that are often overlooked in conventional BTEX-focused monitoring. The TAC concentration reached 41.40 ± 5.20 µg/m3, with half of the compounds exhibiting significant increases during peak commuting periods. Source apportionment using diagnostic ratios and PMF identified five major contributors: carriage material emissions (36.62%), human sources (22.50%), traffic exhaust infiltration (16.67%), organic solvents (16.55%), and industrial emissions (7.66%). Although both non-cancer (HI) and cancer (TCR) risks for all population groups were below international thresholds, summer tourists experienced higher exposure than daily commuters. Notably, child tourists showed the greatest vulnerability, with a TCR of 5.83 × 10−7, far exceeding that of commuting children (1.88 × 10−7). Benzene was the dominant contributor, accounting for over 50% of HI and 70% of TCR. This study presents the first integrated NTS and quantitative risk assessment to characterise ACs in summer metro environments, revealing a broader range of hazardous compounds beyond BTEX. It quantifies population-specific risks, highlights children’s heightened vulnerability. The findings fill critical gaps in ACs exposure and provide a scientific basis for improved air quality management and pollution mitigation strategies in urban rail transit systems. Full article
(This article belongs to the Section Building Energy, Physics, Environment, and Systems)
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22 pages, 1247 KiB  
Article
Evaluating and Predicting Urban Greenness for Sustainable Environmental Development
by Chun-Che Huang, Wen-Yau Liang, Tzu-Liang (Bill) Tseng and Chia-Ying Chan
Processes 2025, 13(8), 2465; https://doi.org/10.3390/pr13082465 - 4 Aug 2025
Viewed by 205
Abstract
With the rapid pace of urbanization, cities are increasingly facing severe challenges related to environmental pollution, ecological degradation, and climate change. Extreme climate events—such as heatwaves, droughts, heavy rainfall, and wildfires—have intensified public concern about sustainability, environmental protection, and low-carbon development. Ensuring environmental [...] Read more.
With the rapid pace of urbanization, cities are increasingly facing severe challenges related to environmental pollution, ecological degradation, and climate change. Extreme climate events—such as heatwaves, droughts, heavy rainfall, and wildfires—have intensified public concern about sustainability, environmental protection, and low-carbon development. Ensuring environmental preservation while maintaining residents’ quality of life has become a central focus of urban governance. In this context, evaluating green indicators and predicting urban greenness is both necessary and urgent. This study incorporates international frameworks such as the EU Green City Index, the European Green Capital Award, and the United Nations Sustainable Development Goals to assess urban sustainability. The Extreme Gradient Boosting (XGBoost) algorithm is employed to predict the green level of cities and to develop multiple optimized models. Comparative analysis with traditional models demonstrates that XGBoost achieves superior performance, with an accuracy of 0.84 and an F1-score of 0.81. Case study findings identify “Greenhouse Gas Emissions per Person” and “Per Capita Emissions from Transport” as the most critical indicators. These results provide practical guidance for policymakers, suggesting that targeted regulations based on these key factors can effectively support emission reduction and urban sustainability goals. Full article
(This article belongs to the Section Environmental and Green Processes)
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18 pages, 2835 KiB  
Article
Numerical Modeling of Gentamicin Transport in Agricultural Soils: Implications for Environmental Pollution
by Nami Morales-Durán, Sebastián Fuentes, Jesús García-Gallego, José Treviño-Reséndez, Josué D. García-Espinoza, Rubén Morones-Ramírez and Carlos Chávez
Antibiotics 2025, 14(8), 786; https://doi.org/10.3390/antibiotics14080786 - 2 Aug 2025
Viewed by 394
Abstract
Background/Objectives: In recent years, the discharge of antibiotics into rivers and irrigation canals has increased. However, few studies have addressed the impact of these compounds on agricultural fields that use such water to meet crop demands. Methods: In this study, the transport of [...] Read more.
Background/Objectives: In recent years, the discharge of antibiotics into rivers and irrigation canals has increased. However, few studies have addressed the impact of these compounds on agricultural fields that use such water to meet crop demands. Methods: In this study, the transport of two types of gentamicin (pure gentamicin and gentamicin sulfate) was modeled at concentrations of 150 and 300 μL/L, respectively, in a soil with more than 60 years of agricultural use. Infiltration tests under constant head conditions and gentamicin transport experiments were conducted in acrylic columns measuring 14 cm in length and 12.7 cm in diameter. The scaling parameters for the Richards equation were obtained from experimental data, while those for the advection–dispersion equation were estimated using inverse methods through a nonlinear optimization algorithm. In addition, a fractal-based model for saturated hydraulic conductivity was employed. Results: It was found that the dispersivity of gentamicin sulfate is 3.1 times higher than that of pure gentamicin. Based on the estimated parameters, two simulation scenarios were conducted: continuous application of gentamicin and soil flushing after antibiotic discharge. The results show that the transport velocity of gentamicin sulfate in the soil may have short-term consequences for the emergence of resistant microorganisms due to the destination of wastewater containing antibiotic residues. Conclusions: Finally, further research is needed to evaluate the impact of antibiotics on soil physical properties, as well as their effects on irrigated crops, animals that consume such water, and the soil microbiota. Full article
(This article belongs to the Special Issue Impact of Antibiotic Residues in Wastewater)
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28 pages, 2266 KiB  
Review
Uncovering Plastic Pollution: A Scoping Review of Urban Waterways, Technologies, and Interdisciplinary Approaches
by Peter Cleveland, Donna Cleveland, Ann Morrison, Khoi Hoang Dinh, An Nguyen Pham Hai, Luca Freitas Ribeiro and Khanh Tran Duy
Sustainability 2025, 17(15), 7009; https://doi.org/10.3390/su17157009 - 1 Aug 2025
Viewed by 264
Abstract
Plastic pollution is a growing environmental and social concern, particularly in Southeast Asia, where urban rivers serve as key pathways for transporting waste to marine environments. This scoping review examines 110 peer-reviewed studies to understand how plastic pollution in waterways is being researched, [...] Read more.
Plastic pollution is a growing environmental and social concern, particularly in Southeast Asia, where urban rivers serve as key pathways for transporting waste to marine environments. This scoping review examines 110 peer-reviewed studies to understand how plastic pollution in waterways is being researched, addressed, and reconceptualized. Drawing from the literature across environmental science, technology, and social studies, we identify four interconnected areas of focus: urban pollution pathways, innovations in monitoring and methods, community-based interventions, and interdisciplinary perspectives. Our analysis combines qualitative synthesis with visual mapping techniques, including keyword co-occurrence networks, to explore how real-time tools, such as IoT sensors, multi-sensor systems, and geospatial technologies, are transforming the ways plastic waste is tracked and analyzed. The review also considers the growing use of novel theoretical frameworks, such as post-phenomenology and ecological materialism, to better understand the role of plastics as both pollutants and ecological agents. Despite progress, the literature reveals persistent gaps in longitudinal studies, regional representation, and policy translation, particularly across the Global South. We emphasize the value of participatory models and community-led research in bridging these gaps and advancing more inclusive and responsive solutions. These insights inform the development of plastic tracker technologies currently being piloted in Vietnam and contribute to broader sustainability goals, including SDG 6 (Clean Water and Sanitation), SDG 12 (Responsible Consumption and Production), and SDG 14 (Life Below Water). Full article
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19 pages, 440 KiB  
Article
Cost-Benefit Analysis of Diesel vs. Electric Buses in Low-Density Areas: A Case Study City of Jastrebarsko
by Marko Šoštarić, Marijan Jakovljević, Marko Švajda and Juraj Leonard Vertlberg
World Electr. Veh. J. 2025, 16(8), 431; https://doi.org/10.3390/wevj16080431 - 1 Aug 2025
Viewed by 178
Abstract
This paper presents a comprehensive analysis comparing the implementation of electric and diesel buses for public transport services in the low-density area of the City of Jastrebarsko in Croatia. It utilizes a multidimensional approach and incorporates direct and indirect costs, such as vehicle [...] Read more.
This paper presents a comprehensive analysis comparing the implementation of electric and diesel buses for public transport services in the low-density area of the City of Jastrebarsko in Croatia. It utilizes a multidimensional approach and incorporates direct and indirect costs, such as vehicle acquisition, operation, charging, maintenance, and environmental impact costs during the lifecycle of the buses. The results show that, despite the higher initial investment in electric buses, these vehicles offer savings, especially when coupled with significantly reduced emissions of pollutants, which decreases indirect costs. However, local contexts differ, leading to a need to revise whether or not a municipality can finance the procurement and operations of such a fleet. The paper utilizes a robust methodological framework, integrating a proposal based on real-world data and demand and combining it with predictive analytics to forecast long-term benefits. The findings of the paper support the introduction of buses as a sustainable solution for Jastrebarsko, which provides insights for public transport planners, urban planners, and policymakers, with a discussion about the specific issues regarding the introduction, procurement, and operations of buses of different propulsion in a low-density area. Full article
(This article belongs to the Special Issue Zero Emission Buses for Public Transport)
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15 pages, 1071 KiB  
Article
A Synthetic Difference-in-Differences Approach to Assess the Impact of Shanghai’s 2022 Lockdown on Ozone Levels
by Yumin Li, Jun Wang, Yuntong Fan, Chuchu Chen, Jaime Campos Gutiérrez, Ling Huang, Zhenxing Lin, Siyuan Li and Yu Lei
Sustainability 2025, 17(15), 6997; https://doi.org/10.3390/su17156997 - 1 Aug 2025
Viewed by 242
Abstract
Promoting sustainable development requires a clear understanding of how short-term fluctuations in anthropogenic emissions affect urban environmental quality. This is especially relevant for cities experiencing rapid industrial changes or emergency policy interventions. Among key environmental concerns, variations in ambient pollutants like ozone (O [...] Read more.
Promoting sustainable development requires a clear understanding of how short-term fluctuations in anthropogenic emissions affect urban environmental quality. This is especially relevant for cities experiencing rapid industrial changes or emergency policy interventions. Among key environmental concerns, variations in ambient pollutants like ozone (O3) are closely tied to both public health and long-term sustainability goals. However, traditional chemical transport models often face challenges in accurately estimating emission changes and providing timely assessments. In contrast, statistical approaches such as the difference-in-differences (DID) model utilize observational data to improve evaluation accuracy and efficiency. This study leverages the synthetic difference-in-differences (SDID) approach, which integrates the strengths of both DID and the synthetic control method (SCM), to provide a more reliable and accurate analysis of the impacts of interventions on city-level air quality. Using Shanghai’s 2022 lockdown as a case study, we compare the deweathered ozone (O3) concentration in Shanghai to a counterfactual constructed from a weighted average of cities in the Yangtze River Delta (YRD) that did not undergo lockdown. The quasi-natural experiment reveals an average increase of 4.4 μg/m3 (95% CI: 0.24–8.56) in Shanghai’s maximum daily 8 h O3 concentration attributable to the lockdown. The SDID method reduces reliance on the parallel trends assumption and improves the estimate stability through unit- and time-specific weights. Multiple robustness checks confirm the reliability of these findings, underscoring the efficacy of the SDID approach in quantitatively evaluating the causal impact of emission perturbations on air quality. This study provides credible causal evidence of the environmental impact of short-term policy interventions, highlighting the utility of SDID in informing adaptive air quality management. The findings support the development of timely, evidence-based strategies for sustainable urban governance and environmental policy design. Full article
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15 pages, 2879 KiB  
Article
Study on the Eye Movement Transfer Characteristics of Drivers Under Different Road Conditions
by Zhenxiang Hao, Jianping Hu, Xiaohui Sun, Jin Ran, Yuhang Zheng, Binhe Yang and Junyao Tang
Appl. Sci. 2025, 15(15), 8559; https://doi.org/10.3390/app15158559 - 1 Aug 2025
Viewed by 166
Abstract
Given the severe global traffic safety challenges—including threats to human lives and socioeconomic impacts—this study analyzes visual behavior to promote sustainable transportation, improve road safety, and reduce resource waste and pollution caused by accidents. Four typical road sections, namely, turning, straight ahead, uphill, [...] Read more.
Given the severe global traffic safety challenges—including threats to human lives and socioeconomic impacts—this study analyzes visual behavior to promote sustainable transportation, improve road safety, and reduce resource waste and pollution caused by accidents. Four typical road sections, namely, turning, straight ahead, uphill, and downhill, were selected, and the eye movement data of 23 drivers in different driving stages were collected by aSee Glasses eye-tracking device to analyze the visual gaze characteristics of the drivers and their transfer patterns in each road section. Using Markov chain theory, the probability of staying at each gaze point and the transfer probability distribution between gaze points were investigated. The results of the study showed that drivers’ visual behaviors in different road sections showed significant differences: drivers in the turning section had the largest percentage of fixation on the near front, with a fixation duration and frequency of 29.99% and 28.80%, respectively; the straight ahead section, on the other hand, mainly focused on the right side of the road, with 31.57% of fixation duration and 19.45% of frequency of fixation; on the uphill section, drivers’ fixation duration on the left and right roads was more balanced, with 24.36% of fixation duration on the left side of the road and 25.51% on the right side of the road; drivers on the downhill section looked more frequently at the distance ahead, with a total fixation frequency of 23.20%, while paying higher attention to the right side of the road environment, with a fixation duration of 27.09%. In terms of visual fixation, the fixation shift in the turning road section was mainly concentrated between the near and distant parts of the road ahead and frequently turned to the left and right sides; the straight road section mainly showed a shift between the distant parts of the road ahead and the dashboard; the uphill road section was concentrated on the shift between the near parts of the road ahead and the two sides of the road, while the downhill road section mainly occurred between the distant parts of the road ahead and the rearview mirror. Although drivers’ fixations on the front of the road were most concentrated under the four road sections, with an overall fixation stability probability exceeding 67%, there were significant differences in fixation smoothness between different road sections. Through this study, this paper not only reveals the laws of drivers’ visual behavior under different driving environments but also provides theoretical support for behavior-based traffic safety improvement strategies. Full article
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19 pages, 950 KiB  
Article
How the Adoption of EVs in Developing Countries Can Be Effective: Indonesia’s Case
by Ida Nyoman Basmantra, Ngurah Keshawa Satya Santiarsa, Regina Dinanti Widodo and Caren Angellina Mimaki
World Electr. Veh. J. 2025, 16(8), 428; https://doi.org/10.3390/wevj16080428 - 1 Aug 2025
Viewed by 213
Abstract
Indonesia’s worsening air pollution and traffic emissions have thrust electric vehicles (EVs) into the spotlight, but what really drives Indonesians to make the switch? This study integrates Protection Motivation Theory with green branding and policy frameworks to explain electric vehicle (EV) adoption in [...] Read more.
Indonesia’s worsening air pollution and traffic emissions have thrust electric vehicles (EVs) into the spotlight, but what really drives Indonesians to make the switch? This study integrates Protection Motivation Theory with green branding and policy frameworks to explain electric vehicle (EV) adoption in Indonesia. Using a nationwide survey (n = 986) and partial-least-squares structural-equation modeling, we test how environmental awareness, consumer expectancy, threat appraisal, and coping appraisal shape adoption both directly and through green brand image (GBI), while perceived policy incentives moderate the GBI–adoption link. The model accounts for 54% of the variance in adoption intention. These findings highlight that combining public awareness campaigns, compelling green brand messaging, and carefully calibrated policy incentives is essential for accelerating Indonesia’s transition to cleaner transport. Full article
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27 pages, 1832 KiB  
Review
Breaking the Traffic Code: How MaaS Is Shaping Sustainable Mobility Ecosystems
by Tanweer Alam
Future Transp. 2025, 5(3), 94; https://doi.org/10.3390/futuretransp5030094 - 1 Aug 2025
Viewed by 184
Abstract
Urban areas are facing increasing traffic congestion, pollution, and infrastructure strain. Traditional urban transportation systems are often fragmented. They require users to plan, pay, and travel across multiple disconnected services. Mobility-as-a-Service (MaaS) integrates these services into a single digital platform, simplifying access and [...] Read more.
Urban areas are facing increasing traffic congestion, pollution, and infrastructure strain. Traditional urban transportation systems are often fragmented. They require users to plan, pay, and travel across multiple disconnected services. Mobility-as-a-Service (MaaS) integrates these services into a single digital platform, simplifying access and improving the user experience. This review critically examines the role of MaaS in fostering sustainable mobility ecosystems. MaaS aims to enhance user-friendliness, service variety, and sustainability by adopting a customer-centric approach to transportation. The findings reveal that successful MaaS systems consistently align with multimodal transport infrastructure, equitable access policies, and strong public-private partnerships. MaaS enhances the management of routes and traffic, effectively mitigating delays and congestion while concurrently reducing energy consumption and fuel usage. In this study, the authors examine MaaS as a new mobility paradigm for a sustainable transportation system in smart cities, observing the challenges and opportunities associated with its implementation. To assess the environmental impact, a sustainability index is calculated based on the use of different modes of transportation. Significant findings indicate that MaaS systems are proliferating in both quantity and complexity, increasingly integrating capabilities such as real-time multimodal planning, dynamic pricing, and personalized user profiles. Full article
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24 pages, 650 KiB  
Article
Investigating Users’ Acceptance of Autonomous Buses by Examining Their Willingness to Use and Willingness to Pay: The Case of the City of Trikala, Greece
by Spyros Niavis, Nikolaos Gavanas, Konstantina Anastasiadou and Paschalis Arvanitidis
Urban Sci. 2025, 9(8), 298; https://doi.org/10.3390/urbansci9080298 - 1 Aug 2025
Viewed by 318
Abstract
Autonomous vehicles (AVs) have emerged as a promising sustainable urban mobility solution, expected to lead to enhanced road safety, smoother traffic flows, less traffic congestion, improved accessibility, better energy utilization and environmental performance, as well as more efficient passenger and freight transportation, in [...] Read more.
Autonomous vehicles (AVs) have emerged as a promising sustainable urban mobility solution, expected to lead to enhanced road safety, smoother traffic flows, less traffic congestion, improved accessibility, better energy utilization and environmental performance, as well as more efficient passenger and freight transportation, in terms of time and cost, due to better fleet management and platooning. However, challenges also arise, mostly related to data privacy, security and cyber-security, high acquisition and infrastructure costs, accident liability, even possible increased traffic congestion and air pollution due to induced travel demand. This paper presents the results of a survey conducted among 654 residents who experienced an autonomous bus (AB) service in the city of Trikala, Greece, in order to assess their willingness to use (WTU) and willingness to pay (WTP) for ABs, through testing a range of factors based on a literature review. Results useful to policy-makers were extracted, such as that the intention to use ABs was mostly shaped by psychological factors (e.g., users’ perceptions of usefulness and safety, and trust in the service provider), while WTU seemed to be positively affected by previous experience in using ABs. In contrast, sociodemographic factors were found to have very little effect on the intention to use ABs, while apart from personal utility, users’ perceptions of how autonomous driving will improve the overall life standards in the study area also mattered. Full article
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18 pages, 1085 KiB  
Article
Composition and Structure of Gut Microbiota of Wild and Captive Epinephelus morio via 16S rRNA Analysis and Functional Prediction
by Grecia Montalvo-Fernández, Joanna M. Ortiz-Alcantara, Claudia Durruty-Lagunes, Laura Espinosa-Asuar, Mariela Beatriz Reyes-Sosa and María Leticia Arena-Ortiz
Microorganisms 2025, 13(8), 1792; https://doi.org/10.3390/microorganisms13081792 - 31 Jul 2025
Viewed by 208
Abstract
The gut microbiota plays an essential role in the host’s metabolism. Its composition and structure depend on biological and environmental factors. This work was designed to identify the composition and structure of the wild and captive red grouper (Epinephelus morio) microbiota [...] Read more.
The gut microbiota plays an essential role in the host’s metabolism. Its composition and structure depend on biological and environmental factors. This work was designed to identify the composition and structure of the wild and captive red grouper (Epinephelus morio) microbiota and make predictions regarding its metabolic functions. Our hypothesis stated that wild and captive individuals would share the most abundant taxonomic groups, forming a core microbiota, and individuals in captivity might have exclusive taxonomic groups. Metagenomic DNA was extracted from the intestinal contents of wild and captive individuals. The 16S rRNA gene was amplified and sequenced using Illumina pair-end technology. QIIME2 pipeline was used for sequence analysis and alpha and beta diversity assessment. PICRUSt was used to infer metabolic functions. Twenty-nine phyla were identified; the most abundant were Pseudomonadota, Bacillota, Fusobacteriota, and Actinomycetota. The dominant genera were Photobacterium, Vibrio, Cetobacterium, and Escherichia-Shigella. The metabolic prediction analysis suggested that the Epinephelus morio gut microbiota is related to food digestion, the immune system, antioxidant enzymes, antibiotic resistance, and vitamin B12 transport. We concluded that the microbiota of E. morio established in captivity is sensitive to environmental changes such as water pollution, which can cause a decrease in diversity. Full article
(This article belongs to the Special Issue Aquatic Microorganisms and Their Application in Aquaculture)
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24 pages, 1087 KiB  
Review
After-Treatment Technologies for Emissions of Low-Carbon Fuel Internal Combustion Engines: Current Status and Prospects
by Najunzhe Jin, Wuqiang Long, Chunyang Xie and Hua Tian
Energies 2025, 18(15), 4063; https://doi.org/10.3390/en18154063 - 31 Jul 2025
Viewed by 334
Abstract
In response to increasingly stringent emission regulations, low-carbon fuels have received significant attention as sustainable energy sources for internal combustion engines. This study investigates four representative low-carbon fuels, methane, methanol, hydrogen, and ammonia, by systematically summarizing their combustion characteristics and emission profiles, along [...] Read more.
In response to increasingly stringent emission regulations, low-carbon fuels have received significant attention as sustainable energy sources for internal combustion engines. This study investigates four representative low-carbon fuels, methane, methanol, hydrogen, and ammonia, by systematically summarizing their combustion characteristics and emission profiles, along with a review of existing after-treatment technologies tailored to each fuel type. For methane engines, unburned hydrocarbon (UHC) produced during low-temperature combustion exhibits poor oxidation reactivity, necessitating integration of oxidation strategies such as diesel oxidation catalyst (DOC), particulate oxidation catalyst (POC), ozone-assisted oxidation, and zoned catalyst coatings to improve purification efficiency. Methanol combustion under low-temperature conditions tends to produce formaldehyde and other UHCs. Due to the lack of dedicated after-treatment systems, pollutant control currently relies on general-purpose catalysts such as three-way catalyst (TWC), DOC, and POC. Although hydrogen combustion is carbon-free, its high combustion temperature often leads to elevated nitrogen oxide (NOx) emissions, requiring a combination of optimized hydrogen supply strategies and selective catalytic reduction (SCR)-based denitrification systems. Similarly, while ammonia offers carbon-free combustion and benefits from easier storage and transportation, its practical application is hindered by several challenges, including low ignitability, high toxicity, and notable NOx emissions compared to conventional fuels. Current exhaust treatment for ammonia-fueled engines primarily depends on SCR, selective catalytic reduction-coated diesel particulate filter (SDPF). Emerging NOx purification technologies, such as integrated NOx reduction via hydrogen or ammonia fuel utilization, still face challenges of stability and narrow effective temperatures. Full article
(This article belongs to the Special Issue Engine Combustion Characteristics, Performance, and Emission)
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