Search Results (531)

Background/Objectives: Psoriasis vulgaris is a chronic inflammatory skin condition with significant psychosocial burden. While phototherapy remains one of the most widely used treatment regimens, novel modalities like blue light therapy offer UV-free alternatives with potentially more favorable safety profiles, but their systemic immunomodulatory effects remain poorly understood. We aimed to evaluate the impact of full-body blue light irradiation on clinical outcomes and selected systemic biochemical and immunological markers in patients with mild-to-moderate psoriasis vulgaris. Methods: This preliminary study involved 21 patients (13 females, 8 males) with mild-to-moderate psoriasis vulgaris. Participants received ten sessions of full-body blue light therapy (453 nm, 40 mW/cm², 30 min per session). Clinical assessments (PASI, PGA, DLQI, VAS, Pruritus Scale) and serum analyses of inflammatory (TNF-α, IL-13, IL-17, IL-31), metabolic (adiponectin, 25(OH)D3), and neuroimmune markers (serotonin, kynurenic acid, quinolinic acid) were performed pre- and post-treatment. Results: Significant improvements were observed in PASI, PGA, DLQI, and pruritus scores (p < 0.05). 25(OH)D3, serotonin, and kynurenic acid levels increased significantly, while IL-31 and IL-17 levels decreased and IL-13 levels increased; TNF-α, adiponectin, and quinolinic acid levels showed no significant changes. Counterintuitively, correlation analysis demonstrated a moderate positive association between changes in IL-13 and PASI improvement (r = 0.51, p = 0.02), while changes in other biochemical parameters were not significantly associated with clinical outcomes. Conclusions: Full-body blue light therapy resulted in significant clinical improvement accompanied by heterogeneous systemic immunometabolic changes. These findings suggest complex, pathway-specific immunomodulation, but this requires further investigation in larger controlled studies. Full article

Donor–acceptor (D–A) molecular systems are gaining increasing attention in cancer imaging and phototherapy due to their tunable optical properties and high photosensitizing efficiency. Encapsulation of such D–A molecules in nano-sized polymeric carriers can enhance the efficiency of antitumor therapy by passive tumor accumulation and controlled drug release. Here, we synthesized two D–A molecules—TTDCV and TTInd—based on triphenylamine with thiophene π-spacers and electron-withdrawing dicyanovinyl or indene-1,3-dione moieties. These molecules were used to preparate nanoparticles (NPs) via nanoprecipitation with amphiphilic polymers—poly(ethylene glycol)-block polylactide methyl ether (PEG-b-PLA) and polyethylene oxide-polypropylene oxide (PEO-PPO-PEO, Pluronic^® F-127). The resulting NPs had spherical morphology, core–shell structure and a tunable mean size (66–139 nm), depending on the polymer type used. Photothermal and photodynamic properties of the NPs were confirmed by intracellular reactive oxygen species generation and efficient heating even under 530 nm low dose irradiation (1 J/cm²), leading to substantial in vitro cytotoxicity against Sk-Br-3 and MCF-7 human breast cancer cells. Pluronic-encapsulated systems showed the strongest effect, reducing IC₅₀ values down to 0.99 µg/mL and achieving phototoxicity indices up to 22, accompanied by increased intracellular accumulation studied by confocal microscopy and flow cytometry. This study establishes relationships between molecular design, encapsulation approaches, and the biological performance of nanoparticles, enabling the rational engineering of D–A-derived nanotherapeutics for precision cancer treatment. Full article

Background: Psoriasis is a chronic systemic disease affecting over 60 million people. While phototherapy is effective, its demanding schedule imposes a significant treatment burden. This study explores the lived experiences and healthcare expectations of patients in specialized nursing-led phototherapy units. Methods: A descriptive phenomenological study was conducted (2019–2022) with 72 participants at a Spanish tertiary hospital. Data from semi-structured interviews were analyzed using inductive thematic content analysis. Results: Nine subtopics emerged within four main thematic areas: (1) a gap between psychological awareness and low knowledge of systemic comorbidities; (2) psychological stress as the primary disease trigger; (3) an adherence-stress cycle causing biographical disruption; and (4) a collective demand for a permanent nursing referent to overcome systemic barriers. Conclusions: Phototherapy’s clinical efficacy is often undermined by its logistical rigor. Achieving skin clearance is insufficient if biographical and systemic gaps remain. Integrated care models, led by specialized dermatology nurses, are essential to provide clinical navigation and support beyond conventional skin treatment. Full article

Ultraviolet (UV) light is emerging as an important tool for biosensing, biomedical signal readout, and dose monitoring because of its strong and selective interactions with nucleic acids, proteins, and other biological components. This review summarizes recent progress in UV sensing-guided biomedical systems, with emphasis on three interconnected directions: label-free and surface-weighted imaging, wearable and embedded UV dosimetry, and sensor-assisted therapeutic guidance. Representative examples include ultraviolet photoacoustic microscopy (UV-PAM) for label-free nuclear imaging, microscopy with ultraviolet surface excitation (MUSE) for rapid slide-free histology-like readout, epidermal and flexible UV dosimeters for skin-level exposure quantification, and UV therapeutic platforms that are increasingly supported by sensing, dosimetry, and feedback for safer dose delivery. Across these applications, we emphasize the shared biosensing principles of signal generation, optical or acoustic transduction, quantitative readout, calibration, and feedback-informed decision support. We also discuss the role of artificial intelligence in virtual staining, image enhancement, domain correction, dose prediction, and decision support. The review concludes with key translational challenges in standardization, uncertainty quantification, multimodal integration, and feedback-driven system design. Overall, this sensing-centered perspective helps define the role of UV technologies more clearly within biosensors-oriented biomedical engineering. Full article

Cutaneous hematologic neoplasms in children are relatively rare and encompass a wide range of lymphoproliferative and myeloproliferative disorders. This review explores and updates the classification, clinical presentation, diagnostic challenges, histopathology, and management of pediatric lymphomas, lymphoproliferations, and leukemias that may be seen in the skin. The most frequent of them are lymphomatoid papulosis (LyP) and mycosis fungoides (MF), and are discussed first, with a particular focus on differential diagnosis and overlaps with benign lesions—mainly pityriasis lichenoides—which raises questions regarding the delineation of these entities and their potential interconnection. It is important to underline that most cutaneous lymphoproliferations are indolent in children: primary cutaneous CD4+ small/medium T-cell lymphoproliferative disorder, subcutaneous panniculitis-like T-cell lymphoproliferation (non-associated with HAVCR2 mutations), primary cutaneous marginal zone lymphoproliferative disorder, and EBV-related lymphoproliferative disorders. However, aggressive hematologic malignancies, although rarer, must not be missed; these are mostly leukemias (but not all forms) and blastic plasmacytoid dendritic cell neoplasm. We emphasize the importance of clinical–pathological correlation, with clonality studies playing a crucial role in some cases. Management strategies are briefly reviewed, ranging from skin-directed therapies like phototherapy and corticosteroids to systemic treatments for more aggressive forms of leukemia cutis and lymphomas. Full article

Background/Objectives: Endometriosis is a chronic condition affecting women of reproductive age. Its symptoms have a negative impact on the quality of life and fertility of many women in the population. The aim of this literature review was to examine the use of phototherapy, heating and acupuncture in the treatment of endometriosis. Methods: A structured review of the available literature using the PubMed, Embase and Scopus databases, including studies from the last 10 years and with full free access, was applied. The literature search was conducted using the keywords: “endometriosis”, “phototherapy”, “heating” and “acupuncture”. Results: Phototherapy, including photothermal (PTT) and photodynamic therapy (PDT), demonstrated promising results in preclinical animal models, suggesting a potential for reducing endometrial lesions, primarily through mechanisms involving apoptosis, necrosis, and oxidative stress. However, most studies were limited to animal models. Thermal interventions, including magnetic hyperthermia and perioperative heating strategies, were associated with pain reduction, although improper use may lead to adverse effects such as erythema. Acupuncture showed effectiveness in reducing pain and improving quality of life, although its effects may be temporary and supported mainly by small-scale studies and case reports. Conclusions: Studies available in the literature demonstrate the effectiveness of the phototherapy effect, utilizing the mechanism of apoptosis or necrosis, in eliminating endometrial tissue and reducing pain. Acupuncture, derived from Traditional Chinese Medicine, also reduces pain. Non-pharmacological interventions may provide supportive benefits in the management of endometriosis, particularly in pain reduction and lesion control. Full article

Immune checkpoint inhibitors (ICIs) have substantially improved outcomes in advanced melanoma but are frequently linked to immune-related adverse events (irAEs). Vitiligo is a common cutaneous irAE and has been consistently associated with improved patient outcome, including prolonged progression-free and overall survival. It also represents significant visual stigma, particularly when the face is involved. Traditional treatment comprises topical steroids, calcineurin inhibitors, laser, and phototherapy which often have insufficient effects. Since 2023, the first approved drug for non-segmental vitiligo (NSV) with facial involvement, the topical Janus kinase inhibitor ruxolitinib, has been available. However, experience with its use in ICI-induced vitiligo remains limited. In this exploratory analysis, three patients who developed facial vitiligo following ICI therapy applied 1.5% ruxolitinib cream to affected facial areas twice daily. After six (two patients), and twelve months (one patient), extensive repigmentation was observed, quantified at 95.7%, 78.9%, and 99.1% using a novel semi-automatic tool. Quality-of-life questionnaires showed mean reductions of 57.6% (Vitiligo DLQI) and 68.2% (Vitiligo-specific Quality of Life) in disease burden. Treatment was associated with substantial repigmentation without observed side effects. Further evaluation in larger, prospective cohorts is warranted to better define treatment effects, clinical applicability, and long-term safety. Full article

Background: The widespread prevalence of neck pain (NP) is a serious healthcare and social problem, and the question of which components of physiotherapy are most effective is still valid. Objectives: The objective of this study was to assess the effect of the type of electrotherapy applied on the outcomes of complex physiotherapy administered to individuals with NP. Methods: In line with the study protocol, 100 individuals with NP were enrolled and randomly divided into four groups. All groups received kinesiotherapy and phototherapy. Additionally, each group also received electrotherapy treatment, which was a differentiating factor. Participants were assessed at baseline, post-intervention, and after six months. The examination involved evaluation of pain using VAS and measurement of the cervical range of motion (ROM). Overall, seven parameters were assessed during each examination. Results: Pain intensity decreased in all individuals across the three study periods. A large effect size and changes exceeding the Minimal Clinically Important Difference (MCID) were observed only in the electrotherapy groups. The improvement in cervical spine ROM was comparable in the HF and LF TENS groups in the short- and long-term perspectives; however, a greater number of effects (p < 0.05) was observed in the HF TENS group. TC resulted only in large and moderate short-term effects reflected by improvements in cervical spine ROM. In the PLACEBO group, moderate long-term effects were observed. Conclusions: Low-frequency currents appear to improve the analgesic effectiveness of complex physiotherapy implemented in individuals with NP. TC may provide better short-term effects compared to long-term effects reflected by improvements in cervical spinal ROM. The effects in the PLACEBO group may suggest that phototherapy and kinesiotherapy are more effective due to the continuation of exercise and the education in ergonomics of work. Full article

Cutaneous leishmaniasis is a protozoan disease which is responsible for significant morbidity in humans. Currently, there is no clinically approved vaccine to prevent infections, and, therefore, treatments to cure skin lesions are required. Ideally, a treatment that can be self-administered to affected areas is desirable. In this study, the effect of violet-blue light, of wavelength in the region of 405 nm, on the survival of Leishmania major and Leishmania mexicana was determined using in vitro and in vivo models. Light treatment caused significant killing of both promastigotes and intracellular amastigotes (p < 0.001) of both species in vitro, and L. mexicana intracellular amastigotes were more resistant to light treatment than L. major intracellular amastigotes. Treatment with violet-blue light at a dose of 45 J/cm² (0.15 W/cm² for 5 min) per day on days 3–7 post-infection in an in vivo footpad model caused a significant reduction in L. major parasite burdens on day 5 post-infection (p < 0.05) in one of two experiments, though by day 10 post-infection, parasite numbers had recovered to those of controls. The results of this study clearly demonstrate that violet-blue light can kill both L. major and L. mexicana parasites, but application to infected cutaneous tissues requires refinement. Full article

Cancer continues to present a profound challenge due to high mortality and the inherent limitations of conventional treatments, including suboptimal targeting, systemic toxicity, and difficulty in overcoming physiological barriers. Micro/nanorobots (MNRs) offer a promising enhanced precision and efficacy in cancer therapy. This review systematically analyzes recent advancements in MNR applications, establishing a consistent framework that interlinks their diverse material compositions, propulsion strategies, and therapeutic functions. We critically compare various materials (inorganic, organic/polymeric, and biological/hybrid materials), elucidating their respective trade-offs in biocompatibility, biodegradability, and stimulus responsiveness. This paper further examines both internal (chemical and biological) and external (magnetic, light, and ultrasound) propulsion mechanisms, highlighting their strengths in overcoming biological barriers and enabling complex in vivo navigation, while also discussing their inherent limitations in control, fuel dependency, and tissue penetration. We then synthesize the therapeutic capabilities of MNRs across targeted drug delivery, phototherapy, radiotherapy, and immunotherapy, emphasizing common advantages like enhanced tumor specificity and reduced systemic side effects. A forward-looking perspective was also provided on the remaining challenges, particularly focusing on in vivo controllability, long-term biosafety, manufacturing scalability, and the significant hurdles in clinical translation. By offering a more critical and integrated analysis, this review underscores the immense potential of MNRs to revolutionize personalized precision cancer treatment, while candidly addressing the complex obstacles that must be surmounted for their successful clinical adoption. Full article

Background: Foreign citizenship and low socioeconomic status are key determinants of health inequalities and may influence maternal and neonatal outcomes. This study aimed to assess maternal health during pregnancy and the main adverse maternal and neonatal outcomes related to labour and childbirth among women living in Trentino (Northern Italy), comparing women with Italian and foreign citizenship. Methods: A retrospective epidemiological study was conducted using data from the Birth Assistance Certificate (CedAP) database of the Autonomous Province of Trento. This study included all women who gave birth in Trentino between 2012 and 2016. Associations between citizenship and adverse outcomes were assessed using multivariable logistic regression models adjusted for potential confounders. Results: The analysis included 23,165 women, of whom 25.9% had foreign citizenship. Women with foreign citizenship showed a significantly higher risk of gestational diabetes mellitus compared with Italian women and an increased risk of extremely preterm birth (<28 weeks of gestation), particularly among women from Central and South America, Asia and Eastern Europe. Regarding labour and mode of delivery, women with foreign citizenship had a higher risk of caesarean section, especially among women from Central and South America and Africa. In terms of neonatal outcomes, infants born to women with foreign citizenship showed a higher likelihood of requiring phototherapy and admission to the neonatal intensive care unit. Conclusions: Significant differences were observed between immigrant and Italian women in both social determinants and maternal and neonatal perinatal outcomes. Identifying factors associated with adverse outcomes during pregnancy may help improve targeted maternal care and reduce health inequalities for both mothers and newborns. Full article

Recent studies have reported skin microbiome dysbiosis in patients with photodermatoses, featuring enriched Staphylococcus aureus colonization and decreased microbiome diversity. We propose that ultraviolet radiation (UVR), along with atypical antimicrobial peptides, may exert selective pressure on the skin microbiome, while cytokine dysregulation and a reduction in commensal bacteria amplify microbial dysbiosis. Dysbiotic microorganisms further release pathogen-associated patterns and virulence factors, and activate tissue-resident memory T cells, which collectively contribute to local inflammation. These mechanisms establish the skin microbiome as a potential target for early intervention. Potential therapeutic strategies may include antibiotics, phototherapy, bleach baths, phage therapy, and microbiota-based therapies. This review integrates current findings from microbial ecology, molecular biology, and host immunology to outline a conceptual framework linking UVR exposure, microbiome alterations, and cutaneous immune responses, while emphasizing the current limitations and evidence gaps in this field. Full article

Atopic dermatitis (AD) is a chronic, relapsing inflammatory dermatosis characterized by pruritus, eczematous lesions, and a fluctuating course. It imposes substantial quality-of-life and economic burdens through sleep disturbance, pain, psychosocial distress, and frequent healthcare utilization. Recent global estimates suggest AD affects hundreds of millions worldwide, with meaningful prevalence in both children and adults. AD pathogenesis is multifactorial, reflecting the interaction of genetic predisposition, immune dysregulation dominated by type 2 inflammation, epidermal barrier impairment, neuroimmune itch pathways, and microbial dysbiosis. Clinical diagnosis remains primarily clinical, supported by classic criteria emphasizing pruritus, typical morphology, chronicity, and atopic history. Disease severity and treatment response are commonly quantified using validated measures such as EASI and SCORAD, enabling standardized monitoring and evidence-based escalation. Management has shifted from broad immunosuppression to a stepwise, endotype-aware approach integrating barrier repair, anti-inflammatory topical therapy, phototherapy, conventional systemic agents, and rapidly expanding targeted options. Recent guidelines and approvals highlight increasing roles for biologics and JAK pathway inhibition, alongside newer nonsteroidal topicals. This review summarizes current concepts and practical treatment integration, with emphasis on safety, monitoring, and future research directions. Full article

Phototherapy is a physical treatment modality that utilizes natural or artificial light sources and harnesses radiant energy to treat diseases. Dynamic monitoring of the actual light dose received by tissues is crucial to the success of phototherapy. However, most current phototherapy devices feature bulky and complex hardware and depend on fixed parameters or surface measurements for dose estimation, failing to provide precise, real-time monitoring of light dose distribution that is tailored to individual users, specific treatment sessions, and different body regions. Furthermore, most of these devices are incapable of generating tunable and stable LED light. This study presents a preliminary diffusion equation-based proof-of-concept for a wearable, integrated dual-mode sensing system for real-time dynamic monitoring of tissue light dose and temperature change. The system, controlled by a single-chip microcontroller, rapidly extracts key tissue optical parameters via a custom multi-wavelength LED optical probe and provides real-time feedback on light dose distribution through a dynamic tissue optical simulation model. To expand the monitoring dimensions, the system innovatively integrates a thermal sensor. This sensor enables synchronous monitoring of the temperature field in the treatment area, thereby allowing for an estimation of the combined photothermal effect. The system features a compact design, user-friendly operation, fast and stable communication, and repeatable and reliable detection. With promising clinical application prospects, it holds the potential to evolve into a portable, home-use, safe, effective, wearable, and cost-effective phototherapy device. Full article

Comano thermal water (CTW) is a hypotonic, bicarbonate–calcium–magnesium mineral water traditionally used to manage chronic inflammatory and relapsing skin diseases. This review summarises and discusses the available clinical, experimental, and translational evidence on CTW, with a particular focus on dermatological indications. The physicochemical properties of CTW, along with the presence of a stable, non-pathogenic microbial community, are examined in relation to their potential biological activity. Clinical studies indicate that CTW-based balneotherapy, alone or in combination with narrowband Ultraviolet B (UVB) phototherapy, is associated with improvements in disease severity, symptom burden, and quality of life in patients with psoriasis and atopic dermatitis, and has a favourable safety and tolerability profile. Experimental data further suggest that CTW may exert anti-inflammatory and immunomodulatory effects, modulate keratinocyte function, support skin barrier restoration, and influence the cutaneous microenvironment, including microbiome-related pathways. The review also outlines emerging evidence for CTW in skin regeneration and in upper airway inflammatory conditions treated via inhalation-based approaches. Overall, this review suggests that CTW may serve as a biologically active therapeutic resource, warranting further investigation as a complementary approach within integrative management strategies for inflammatory and barrier-related conditions. Full article