Search Results (232)

The two-stage proportional valve is a key control component in heavy-duty equipment, where its signal-flow characteristics critically influence operational performance. This study proposes an innovative flow characteristic regulation method using variable-gain feedback grooves. Unlike conventional throttling notch optimization, the core mechanism actively adjusts pilot–main valve mapping through feedback groove shape and area gain adjustments to achieve the desired flow curves. This approach avoids complex throttling notch issues while retaining the valve’s high dynamics and flow capacity. Mathematical modeling elucidated the underlying mechanism. Subsequently, trapezoidal and composite feedback grooves are designed and investigated via simulation. Finally, composite feedback groove spools tailored to construction machinery operating conditions are developed. Comparative experiments demonstrate the following: (1) Pilot–main mapping inversely correlates with area gain; increasing gain enhances micro-motion control, while decreasing gain boosts flow gain for rapid actuation. (2) This method does not significantly increase pressure loss or energy consumption (measured loss: 0.88 MPa). (3) The composite groove provides segmented characteristics; its micro-motion flow gain (2.04 L/min/0.1 V) is 61.9% lower than conventional valves, significantly improving fine control. (4) Adjusting groove area gain and transition point flexibly modifies flow gain and micro-motion zone length. This method offers a new approach for high-performance valve flow regulation. Full article

The experimental tests needed for the estimation of the fatigue limit generally require extensive time and many specimens. A valid but not standardized alternative is the thermographic analysis of the self-heating phenomenon. The present work is aimed at using Infrared thermography to determine the fatigue limit in two kinds of Ti-6Al-4V samples obtained by hot rolling: (1) with the standard dog-bone shape (unnotched specimen) and (2) with two opposed semicircular notches at the sides (notched specimen). Uniaxial tensile experiments are performed on unnotched samples, and the surface temperature variation during loading is monitored. The stress corresponding to the end of the thermoelastic stage gives a rough indication of the fatigue limit. Then, fatigue tests at different sinusoidal loads are performed, and the thermographic signal is monitored and processed. The results obtained using lock-in thermography in dissipative mode, e.g., analyzing the second harmonic, showed a sudden change in slope when the applied stress exceeded a certain limit. This slope change is related to the fatigue limit. In addition, the ratio between the fatigue limits obtained for notched and unnotched specimens, e.g., the fatigue strength reduction factor, is consistent with literature values based on the selected geometry. Full article

The control of following motion under mesoscale gap flow fields has important applications. The flexible characteristics of the plant, wideband time-varying disturbances caused by the flow field, and requirements of high precision and low overshoot make achieving submicron level accuracy a significant challenge for traditional control methods. This study adopts the control concept of Disturbance Observer Control (DOBC) and uses H_∞ mixed-sensitivity shaping technology to design a Q-filter. Simultaneously, multiple control techniques, such as high-order reference trajectory planning, Proportional-Integral-Derivative (PID) control, low-pass filtering, notch filtering, lead lag correction, and disturbance rejection filtering, are applied to obtain a control system with a high open-loop gain, sufficient phase margin, and stable closed-loop system. Compared to traditional control methods, the new method can increase the open-loop gain by 15 times and the open-loop bandwidth by 8%. We even observed a 150-time increase of the open-loop gain at the peak frequency. Ultimately, the method achieves submicron level accuracy, making important advances in solving the control problem of semiconductor equipment. Full article

Exosomes, small extracellular vesicles secreted by various cell types, have gained significant attention in cancer investigations. Isolation and characterization of exosomes derived from DOK (dysplastic oral keratinocyte), SCC (squamous cell carcinoma) and HaCaT (normal skin keratinocyte) cell lines and microRNA profiling were conducted. Magnetic sorting was applied to obtain pure exosomes. Morphology and size were characterized by transmission electron microscopy and nanoparticle tracking analysis. Validation of membrane exosomal markers (CD9, CD63) was performed via Western blotting. MiR-21, miR-31, and miR-133 levels were analyzed in exosomes and parent cells by qPCR. Biological effects of the exosomes were tested by adding them to fibroblast cultures and determining the expression of relevant carcinogenesis markers by qPCR. Exosomes appeared as cup-shaped nano-sized particles, and there was no difference regarding particle diameter and concentration between the three types of exosomes. The oncogenic miR-21 was significantly upregulated both in SCC and SCC-derived exosomes compared to DOK and HaCaT cells and their respective exosomes. However, miR-31 unexpectedly showed the highest expression in normal cells and the lowest in HaCaT exosomes. MiR-133, the tumor suppressor miRNA, was downregulated in both SCC and DOK cells compared to normal (HaCaT) cells, while the opposite situation was observed in exosomes, with HaCaT cells showing the lowest levels of miR-133. The differences in exosome content were reflected in signaling pathway activation in exosome-treated fibroblasts, with SCC exosomes exerting the most potent effect on several cancer-related pathways, notably PIK3/AKT, PTEN, and NOTCH signaling cascades. Full article

Vermipsyllid parasitize the body surface of sheep, feeding on blood and transmitting diseases, causing severe economic losses to the livestock industry. An outbreak of sheep Vermipsyllid fleas in the mountain pastures of Xinjiang’s Altai region showed that several commonly used commercial anthelmintic drugs had poor therapeutic effects on the prevalent flea species. This study first conducted species identification of Vermipsyllid through morphological analysis of 200 female and 40 male specimens collected from the Altai region, followed by molecular biological identification of 6 randomly selected fleas (3 females and 3 males). Finally, pharmacodynamic experiments were performed to screen for highly effective anthelmintic drugs. Ninety Altai multiparous ewes infected with Vermipsyllid were divided into six groups (fifteen sheep per group): an untreated control group (Ctr), avermectin injection group (Group I), ivermectin injection group (Group II), moxidectin pour-on group (Group III), cypermethrin pour-on group (Group IV), and dichlorvos pour-on group (Group V), with a 14-day trial period. On Day 0, each group received a single treatment according to drug instructions and specified dosages. The number of fleas, flea population reduction rates, and cure rates were measured on Day 0, Day 3, Day 7, and Day 14 to screen for effective anthelmintic drugs. Results showed that unfed female and male fleas were grayish-brown. Engorged females reached 14.15 mm in size, appearing pale white or yellow, with their sterna maintaining the original size and shape despite abdominal distension. Female fleas had 20–21 segments on the labium, while males had 16–19 segments. The hind tibiae featured four notches, and each side of the tergite had 13 ± 1 cup-like indentations. Molecular biological identification indicated that the identified fleas belonged to D. ioffi (Vermipsyllidae, Dorcadia Ioff), showing 99.13% sequence similarity with D. ioffi from Xinjiang, China. In pharmacodynamic experiments, the number of D. ioffi in Groups IV and V decreased to zero on both Day 7 and Day 14, which was extremely significantly lower than other groups (p < 0.01). The flea population reduction rates in Groups IV and V reached 100% on both days. By Day 14, the cure rates of Groups I, II, and III were 0%, while those of Groups IV and V were 100%. Avermectin injection, ivermectin injection, and moxidectin pour-on showed poor anthelmintic effects, whereas cypermethrin and dichlorvos pour-on exhibited high anthelmintic activity against this flea species. Full article

Background: The intercondylar notch (IN) houses the central ligaments of the knee joint, namely the anterior and posterior cruciate ligaments (ACL and PCL) as well as the anterior and posterior meniscofemoral ligaments (aMFL and pMFL). As not only the available intercondylar space directly influences the encased ligaments, but also the ligaments themselves may influence each other, the purpose of this study was to evaluate the influence of osteoarthritis on central ligament morphology. Methods: Imaging data from the osteoarthritis initiative was used to assess 415 randomly selected patients, equally distributed across five groups based on osteoarthritis severity using the Kellgren and Lawrence classification. MRI scans were used to measure ligament structures in the coronal, axial and sagittal planes. The ACL was evaluated and classified into healthy, pathologic and ruptured. The relationship between osteoarthritis severity and the shape of the IN (A-shape, inverse-U-shape and Ω-shape) was analyzed in relation to ligament morphometrics and ACL condition. Results: The morphology of the ligaments is directly influenced by the development of osteoarthritis. In particular, the Ω-shape, which is associated with severe-grade osteoarthritis, is a risk factor for the development of ACL rupture (p < 0.001). But also, the condition of the ACL influenced the morphometrics of the posterior ligaments, and the PCL as well as the MFLs influenced each other. Conclusions: Statistically significant morphological changes to the encased ligaments in the intercondylar space in osteoarthritis were reported. In particular, the ACL shows a higher risk for pathological changes during ongoing joint degeneration due to osteoarthritis. The other evaluated ligaments—MFLs and PCL—are influenced by the condition of the osseous structures and the shape of the IN as well as by the condition and continuity of the ACL. Full article

Fatigue failure, generated by local multi-axial random state stress, frequently occurs in many engineering fields. Therefore, it is customary to perform experimental vibration tests for a structural durability assessment. Over the years, a number of testing methodologies, which differ in terms of the testing machines, specimen geometry, and type of excitation, have been proposed. The aim of this paper is to describe a new testing procedure for random multi-axial fatigue testing. In particular, the paper presents the experimental set-up, the testing procedure, and the data analysis procedure to obtain the multi-axial random fatigue life estimation. The originality of the proposed methodology consists in the experimental set-up, which allows performing multi-axial fatigue tests with different normal-to-shear stress ratios, by choosing the proper frequency range, using a single-axis exciter. The system is composed of a special designed specimen, clamped on a uni-axial shaker. On the specimen tip, a T-shaped mass is placed, which generates a tunable multi-axial stress state. Furthermore, by means of a finite element model, the system dynamic response and the stress on the notched specimen section are estimated. The model is validated through a harmonic acceleration base test. The experimental tests validate the numerical simulations and confirm the presence of bending–torsion coupled loading. Full article

To investigate the mechanical behavior and damage mechanism of notch–stud connectors in timber–concrete composites under fatigue loading, fifteen push-out specimens in five groups were designed with load cycles as the key variable. Fatigue failure modes and mechanisms were analyzed to examine fatigue life, stiffness degradation, and cumulative damage laws of connectors. Numerical simulations with up to 100 load cycles explored timber/concrete damage effects on stud fatigue performance. Based on the results, an S-N curve was established, a fatigue damage model developed, and a fatigue design method proposed for such connectors. Primary failure modes were stud fracture and local concrete crushing in notches. Stiffness degradation followed an inverted “S”-shaped “fast–slow–fast” pattern. Using residual slip as the damage variable, a two-stage fatigue damage evolution model was constructed from the damage–cycle ratio relationship, offering a new method for shear connector fatigue damage calculation in timber–concrete composites and enabling remaining life prediction for similar composite beam connectors. Finite element simulations of push-out specimens showed high consistency between calculated and experimental fatigue life/damage results, validating the conclusions. Full article

The origin and evolution of genes are central themes in evolutionary biology and genomics, shedding light on how molecular innovations shape biological complexity and adaptation. This review explores the principal mechanisms underlying gene emergence in eukaryotes, including gene duplication, de novo gene birth, horizontal gene transfer, viral gene domestication, and exon shuffling. We examine the population dynamics that govern the fixation of new genes, their functional integration, and the selective forces acting upon them—from purifying selection to adaptive innovation. Examples such as NOTCH2NL and SRGAP2C, which originated through recent segmental duplications followed by neofunctionalization, illustrate how duplicate-derived de novo genes can play a key role in human brain development. In addition, we highlight the emerging relevance of nuclear architecture in determining the evolutionary fate of new genes, offering a spatial dimension to gene innovation. We also discuss methodological approaches for detecting new genes and inferring selection, and finally, we highlight the emerging role of the human pangenome in revealing hidden gene diversity and its implications for evolutionary and biomedical research. Understanding gene innovation not only enhances our grasp of evolutionary processes but also informs clinical studies on disease susceptibility and human uniqueness. Full article

Eddy current testing (ECT) has become a widely adopted technique for non-destructive testing (NDT) due to its effectiveness in detecting surface and near-surface defects in conductive materials. However, traditional methods mainly focus on defect detection and face significant challenges in extracting geometric information such as defect size and shape, which is crucial for structural health monitoring (SHM) and remaining useful life (RUL) assessment. To address these challenges, this study proposes a defect reconstruction approach based on a complex-valued convolutional neural network (CV-CNN), which directly leverages both amplitude and phase information inherent in complex-valued impedance signals. The proposed framework employs convolution, pooling, and activation operations specifically designed within the complex-valued domain to facilitate the high-fidelity reconstruction of defect morphology as well as precise multi-class defect classification. Notably, this approach processes the complete complex-valued signal without relying on prior structural parameters or baseline data, thereby achieving substantial improvements in both defect visualization and classification performance. Moreover, when compared to a complex-valued fully convolutional neural network (CV-FCNN), CV-CNN demonstrates a superior average classification accuracy of 85%, significantly outperforming the CV-FCNN model. Experimental results on carbon steel specimens with standard electrical discharge machining (EDM) notches under multi-frequency excitation confirm these advantages. This contribution provides a promising solution in the field of NDT for intelligent and precise defect detection. Full article

In order to enhance the durability of concrete frame beams, a U-shaped engineered cementitious composites (ECC) protective layer is applied at the end of the frame beams. The bond between the ECC protective layer and the concrete is reinforced by incorporating notches and grooves in the occupancy plate. The development and resistance to cracking of reinforced concrete (RC) frame beams and frame beams with an ECC protective layer were investigated using monotonic loading tests. The test results show that the average value of crack spacing in the negative moment zone of the RC frame beam specimen is in close agreement with the crack spacing calculated according to the GB50010 Code for Design of Concrete Structures. While the dispersion of crack width in the negative moment zone of the RC frame beam specimens is considerable, the distribution pattern of crack width undergoes a gradual change with increasing load. When the maximum crack width calculation method of GB50010 is employed in the negative moment zone of RC frame beams, the crack width should be increased by approximately 1.25 times. Furthermore, the crack spacing and crack width of the ECC protective layer are markedly smaller than those of RC frame beams. Full article

This paper introduces a novel-shaped, compact, multiband monopole antenna sensor incorporating an irregular curved split-ring resonator (SRR) design for non-invasive, continuous monitoring of human blood glucose levels (BGL). The sensor operates at multiple resonance frequencies: 0.94, 1.5, 3, 4.6, and 6.3 GHz, achieving coefficient reflection impedance bandwidths ≤ −10 dB of 4%, 1%, 3.5%, 65%, and 50%, respectively. Additionally, novel shapes of two SRR metamaterial cells create notches at 1.7 GHz and 4.4 GHz. The antenna is fabricated on an economical FR4 substrate with compact dimensions of 35 × 50 × 1.6 mm³. The sensor’s performance is evaluated using 3D electromagnetic software, incorporating a human finger phantom model and applying the Cole–Cole model to mimic the blood layer’s sensitivity to blood glucose variations. The phantom model is positioned at different angles relative to the biosensor to detect frequency shifts corresponding to different glucose levels. Experimental validation involves placing a real human finger around the sensor to measure resonant frequency, magnitude, and phase changes. The fabricated sensor demonstrates a superior sensitivity of 24 MHz/mg/dL effectiveness compared to existing methods. This emphasizes its potential for practical, non-invasive glucose monitoring applications. Full article

This paper aims to investigate the effectiveness of cutting fluid during the high-speed face-turning of superalloy Inconel 718 using chamfered whisker-reinforced ceramic inserts. It addresses this topic by providing a comprehensive understanding of the machinability of Inconel 718 under both dry and wet conditions through analytical friction modeling and a detailed analysis of the chip formation process. Two new indexes, named the Area Function (AF) and the Shape Function (SF), were derived to assess the serration intensity of the chips. Particular attention was paid to the interaction between the cutting speed and the cutting fluid. The results showed that wet conditions promote uniform chip formation, more stable forces, a lower coefficient of friction, and the absence of notch wear. At low cutting speed (60 m/min) and dry machining results in high serration intensity (SF = 0.7) and segmentation frequency ($f_{seg}$ = 22.08 kHz) compared to the SF of 0.4 and $f_{seg}$ = 19.69 kHz in wet conditions. The segmentation frequency increases significantly with cutting speed, reaching 71.03 kHz and 63.32 kHz at a cutting speed of 225 m/min for dry and wet conditions, respectively. It was also found that the rate of increase in the tangential force was lower (20.49 N/s) when using cutting fluid at a high cutting speed (225 m/min) compared to dry conditions (27.37 N/s). Full article

This paper presents the results of experimental and numerical analysis of fracture mechanics testing of ductile metallic materials using a non-standard procedure with PRNT (pipe ring notched tensile) ring-shaped specimens, introduced in previous publications through analysis of 3D-printed polymer rings. The main focus of this research is the determination of the values of the plastic geometry factor η_pl since the specimen is not a standard one. Toward this aim, the finite element software package Simulia Abaqus was applied to evaluate the J-integral (by using the domain integral method) and the F-CMOD curve so that the plastic geometry factor η_pl can be evaluated for different values of the ratio of crack length to specimen width (a₀/W = 0.45 ÷ 0.55). In this way, a procedure and the possibility of practical implementation on the thin-walled pipelines are established. Full article

Ovarian cancer (OC) is a highly heterogeneous malignancy, often characterized by complex genomic alterations that drive tumor progression and therapy resistance. In this paper, we report a novel de novo BRCA2 germline variant NM_000059.3:c.(8693_8695delinsGT) associated with early-onset OC that featured two regions with differential MMR (Mismatch Repair) gene expression. To date, only six cases of de novo BRCA2 variants have been reported, none of which were associated with early-onset high-grade serous OC. The immunohistochemical analysis of MMR genes revealed two distinct tumor areas, separated by a clear topographic boundary, with the heterogeneous expression of MLH1 and PMS2 proteins. Seventy-five percent of the tumor tissue showed positivity, while the remaining 25% exhibited a complete absence of expression, underscoring the spatial variability in MMR gene expression within the tumor. Integrated comparative spatial genomic profiling identified several tumor features associated with the genetic variant as regions of loss of heterozygosity (LOH) that involved BRCA2 and MLH1 genes, along with a significantly higher mutational tumor burden in the tumor area that lacked MLH1 and PMS2 expression, indicating its further molecular evolution. The following variants were acquired: c.6572C>T in NOTCH2, c.1852C>T in BCL6, c.191A>T in INHBA, c.749C>T in CUX1, c.898C>A in FANCG, and c.1712G>C in KDM6A. Integrated comparative spatial proteomic profiles revealed defects in the DNA repair pathways, as well as significant alterations in the extracellular matrix (ECM). The differential expression of proteins involved in DNA repair, particularly those associated with MMR and Base Excision Repair (BER), highlights the critical role of defective repair mechanisms in driving genomic instability. Furthermore, ECM components, such as collagen isoforms, Fibrillin-1, EMILIN-1, Prolargin, and Lumican, were found to be highly expressed in the MLH1/PMS2-deficient tumor area, suggesting a connection between DNA repair deficiencies, ECM remodeling, and tumor progression. Thus, the identification of the BRCA2 variant sheds light on the poorly understood interplay between DNA repair deficiencies and ECM remodeling in OC, providing new insights into their dual role in shaping tumor evolution and suggesting potential targets for novel therapeutic strategies. Full article