Search Results (10,948)

Background/Objectives: The gut microbiota is increasingly recognized as a key determinant of human health, playing a vital role in metabolism, immunity, and disease susceptibility. Dysbiosis, or microbial imbalance, is associated with gastrointestinal disorders such as irritable bowel syndrome (IBS), inflammatory bowel disease (IBD), and Clostridioides difficile infection (CDI), as well as extraintestinal conditions, including obesity, cardiovascular disease, and neuropsychiatric disorders. This review aims to provide an updated overview of emerging therapeutic strategies to modulate the gut microbiota to restore eubiosis and improve health outcomes. Methods: A narrative review of recent literature was conducted, focusing on preclinical and clinical studies investigating microbiota-targeted therapies. The review primarily covers innovative interventional approaches, including fecal microbiota transplantation (FMT), bacterial consortium transplantation (BCT), bacteriophage therapy and outer membrane vesicles (OMVs). Results: Evidence supports the role of probiotics, prebiotics, and synbiotics in remodeling microbial communities and improving host health, although their effects may be strain- and context-dependent. FMT has demonstrated high efficacy in the treatment of recurrent Clostridium difficile infections and is being studied for IBD, IBS and extraintestinal diseases, following the recent Food and Drug Administration approval of the first commercial FMT products. BCT offers a standardized alternative to donor-derived material, with early clinical successes such as FDA-approved SER-109. Phage therapy and OMVs represent promising frontiers, offering targeted microbial modulation and interactions with the immune system, although clinical data remain limited. Conclusions: Emerging gut microbiota modulation strategies offer new perspectives for precision medicine and could transform the prevention and treatment of many diseases, but further studies are needed to ensure their safety, standardization, and clinical application. Full article

Soil management is crucial for addressing soil-borne pathogens, weeds, and pests, ensuring sustainable crop productivity. Traditional chemical fumigants, such as methyl bromide, have been effective but pose serious environmental risks, including ozone depletion and reduced soil biodiversity. Consequently, attention has shifted toward more sustainable alternatives. Techniques like soil solarization, anaerobic soil disinfestation (ASD), biofumigation, and the use of biological control agents (BCAs) offer environmentally friendly options for managing soil-borne diseases. Steam and microwave disinfestation are also promising techniques; however, further development is required to improve their practical efficiency. Integrated management approaches, which combine multiple interventions, have proven particularly effective, offering flexibility and enhancing control through complementary techniques. Additionally, emerging technologies such as artificial intelligence and hyperspectral imaging provide new opportunities for real-time monitoring and decision-support to optimize the timing and targeting of pest management interventions. This review emphasizes the potential of sustainable soil pest control methods to reduce reliance on chemical fumigants, improve crop yield and quality, and support environmentally responsible farming practices. It also examines the challenges associated with scalability, cost, and variable effectiveness, while outlining the strengths, weaknesses, and mechanisms of each method. Further research on regional adaptation, technological integration, and long-term impacts is essential to fully optimize these innovative solutions for food security and sustainable agriculture. Full article

Micro- and nanoplastics (MNPs) are a new type of pollutant that are widely present in terrestrial ecosystems due to agricultural plastics, sludge use, deposition, and litter degradation. Plants can absorb them through the soil and atmosphere, with adverse effects on plant growth and development. Several studies have reported the effects of MNPs on plant physiology, biochemistry, and toxicity. However, the food chain risk of plant uptake of MNPs has not been systematically studied. This review synthesizes current research on plant MNP pollution, focusing on the uptake and transport mechanisms of MNPs by plants, influencing factors, and health hazards. The size, type, and surface charge characteristics of MNPs, as well as environmental conditions, are key factors affecting MNP absorption and accumulation in plants. Furthermore, when MNP-enriched plants are consumed by humans and animals, the accumulated MNPs can diffuse through the bloodstream to various organs, impairing physiological functions and causing a range of health problems. While a comprehensive, traceable investigation of the transmission of MNPs through the terrestrial food chain remains unconfirmed, health risk signals are unequivocal—dietary intake is the primary route of human exposure to MNPs, with direct evidence of their bioaccumulation in human tissues. Addressing this critical research gap, i.e., systematically verifying the full terrestrial food chain translocation of MNPs, is therefore pivotal for conducting robust and comprehensive assessments of the food safety and health risks posed by MNPs. This study analyzed a total of 154 literature sources, providing important theoretical insights into the absorption, transport, and accumulation of MNPs in plants, as well as the health risks associated with their transfer to humans through the food chain. It is expected to provide valuable reference for the research on the transfer of MNPs in the “soil-plant-human” chain. Full article

Background/Objectives: Current data-driven dietary pattern methods have limitations in identifying disease-specific dietary structures. We developed network-derived dietary scores based on type 2 diabetes (T2D)-differential food co-consumption networks and examined their associations with incident T2D risk. Methods: Using the Korean Genome and Epidemiology Study-CArdioVascular disease Association Study (KoGES-CAVAS, n = 16,665), we constructed food co-consumption networks from cumulative average intakes stratified by incident T2D status. The network centrality scores from edges appearing exclusively in either T2D or non-T2D networks were used to generate a differential co-consumption network-derived (D_CCN) score, with higher scores indicating a greater alignment with diabetes-specific structures. CAVAS-derived scores were applied to the Health Examinee Study (KoGES-HEXA, n = 51,206) for cross-cohort validation. Incidence rate ratios (IRRs) were estimated using modified Poisson regression with robust error estimation. Results: During follow-up, 953 and 2190 new cases of T2D were identified in CAVAS and HEXA, respectively. Rice and vegetable dishes were primary hub foods in both networks, with rice showing exclusively negative correlations. Non-T2D networks were more complex, whereas T2D networks were simpler and centered on refined flour-based foods. The D_CCN score was associated with a higher T2D risk in CAVAS (IRR = 1.45, 95% CI: 1.21–1.74), and this association was validated in HEXA (IRR = 1.58, 95% CI: 1.40–1.78), with consistent dose–response relationships (both p-trend < 0.0001). Conclusions: Differential network analysis identified T2D-specific co-consumption structures, and the D_CCN score consistently predicted T2D risk across cohorts. This approach highlights the utility of network-based methods for capturing disease-relevant dietary structures beyond traditional approaches. Full article

Background/Objectives: Few studies have simultaneously evaluated spatial accessibility to both food destinations and spaces for physical activity in relation to body weight in children. The aim of this study was to determine whether spatial accessibility to food destinations and places for physical activity is associated with body weight among children, differentiating between boys and girls. Neighborhood socio-economic status and walkability were incorporated as covariates. Methods: This cross-sectional study was conducted in Valencia, Spain. The initial sample comprised a sample of 808 children. GIS-based procedures were used to evaluate access to food outlets, walkability and socio-economic status (i.e., household income) among home neighborhoods. Access to different types of food destinations and destinations where children can engage in physical activity was assessed using the Neighborhood Environment Walkability Scale for Youth (NEWS-Y-IPEN). Weight and height were objectively assessed. The body mass index (BMI) percentile was calculated. Mixed-model regression analyses were performed. Results: Being a boy was positively associated with BMI percentile (p = 0.003), whereas physical activity was negatively related to this outcome (p = 0.028) in the whole sample. None of the built environment attributes were significantly associated with BMI percentile in boys. Access to healthy restaurants (p = 0.035), as well as neighborhood income (p = 0.049), were negatively associated with BMI percentile among girls. Conclusions: The relationship between built environmental attributes and BMI varies significantly between boys and girls. Understanding these differences is key for designing effective public health interventions with the aim of reducing childhood obesity. Full article

The main characteristics of the large number of coffee species are differences in aroma and caffeine content. Labeled blends of Coffea arabica (C. arabica) and Coffea canephora (C. canephora) are common to broaden the flavor profile or enhance the stimulating effect of the beverage. New emerging species such as Coffea liberica (C. liberica) further increase the variability in blends. However, significant price differences between coffee species increase the risk of unlabeled blends and thus influence food quality and safety for consumers. In this study, a prototypic hyphenation of trapped headspace-gas chromatography-ion mobility spectrometry-quadrupole mass spectrometry (THS-GC-IMS-QMS) was used for the detection of characteristic compounds of C. arabica, C. canephora, and C. liberica in green and roasted coffee samples. For the discrimination of coffee species with IMS data, multivariate resolution with multivariate curve resolution–alternating least squares (MCR-ALS) prior to partial least squares–discriminant analysis (PLS-DA) was evaluated. With this approach, the classification accuracy, as well as sensitivity and specificity, of the PLS-DA model was significantly improved from an overall accuracy of 87% without prior feature selection to 92%. As MCR-ALS preserves the physical and chemical properties of the original data, characteristic features were determined for subsequent substance identification. The simultaneously generated QMS data allowed for partial annotation of the characteristic volatile organic compounds (VOC) of roasted coffee. Full article

Liver fibrosis is a significant consequence of severe liver injury resulting from viral hepatitis, alcohol, and metabolic dysfunction. Progressive fibrosis and ultimate cirrhosis are leading causes of morbidity and mortality worldwide, generally irreversible and poorly targeted by current therapies. Traditional in vitro models and animal models mostly fail to fully recapitulate human multicellular crosstalk, extracellular matrix (ECM) remodeling, and the chronic, immune modulated nature of the disease. Recent advances in three-dimensional (3D) cell culture models including organoids, spheroids, bioprinted constructs, and organ-on-a-chip systems are advantageous for reconstructing cellular diversity and mechanical microenvironments to understand pathophysiology and aid in drug discovery. Emerging multi-organ models are capable of incorporating microbiome derived cues and using multi-omics readouts and imaging-enabled mechanistic dissection for more predictive anti-fibrotic screening. These technologies align well with the recent Modernization 3.0 regulation and New Approach Methodologies by the Food and Drug Administration (FDA) and recent EU Pharmaceutical Reform. This review summarizes the pathophysiology of liver fibrosis, the current landscape of 3D human liver models, and examines how microbiome interfaces modulate fibrogenesis. Full article

Originating in East Asia, the adzuki bean (Vigna angularis) is a diploid crop mainly grown in this region that belongs to the Fabaceae family. As a potential functional food resource with both medicinal and nutritional value, it offers various health benefits. However, research on its chemical constituents, particularly key bioactive components such as triterpenoid saponins and flavonoids, remains relatively limited. In this study, a new triterpenoid saponin, adzukisaponin A (1), along with eleven known compounds (2–12), were isolated from adzuki bean (V. angularis). Among them, compounds 3 (yunganoside B1), 6 (3β-acetyl oleanolic acid), and 7 (β-amyrin) are reported from this source for the first time. Saponins 1–3 and flavonoid 8 exhibited significant dual inhibitory activity. Notably, saponins 1–3 inhibited pancreatic lipase (IC₅₀ = 0.11 ± 0.18 to 0.40 ± 0.21 mM) more strongly than the positive control orlistat, while also acting against α-glucosidase (IC₅₀ = 0.14 ± 0.11 to 0.23 ± 0.17 mM). Molecular docking supported their binding to the enzymes’ active sites. This study identifies 1–3 and 8 as dual α-glucosidase/pancreatic lipase inhibitors, underscoring the potential of adzuki bean as a source of bioactive functional food ingredients. Full article

This study aimed to develop natural, safe, and effective antimicrobial packaging materials for extending the shelf life of chilled pork during refrigeration. Emodin-chitosan (Em-Cs) composite films with varied concentrations were developed by combining the casting method with photodynamic inactivation technology, utilizing chitosan as the matrix and emodin as the functional photosensitizer for active packaging. The optical, mechanical, and barrier properties of the composite films were examined. The inhibitory effects of the samples on Escherichia coli, Salmonella Derby, Staphylococcus aureus, and Pseudomonas fragi under 450 nm blue light irradiation were evaluated. The results demonstrated that the Em-Cs composite film exhibited excellent transparency, mechanical strength, and water barrier properties, with good compatibility between emodin and chitosan. Under light irradiation, the composite film generates reactive oxygen species (ROS), whose bactericidal efficacy depends on the concentration of emodin and the duration of light exposure. When applied to chilled pork packaging, this composite film inhibited bacterial growth within the meat for 10 days, effectively retarding pH increase, lipid oxidation, and volatile basic nitrogen accumulation. The present study proposes a novel methodology for the application of photodynamic technology in the context of food preservation, and it presents a new type of natural antimicrobial packaging material for the preservation of chilled pork. Full article

Background/Objectives: Sodium-glucose co-transporter 2 (SGLT2) inhibitors are essential antidiabetic medications. However, their side effects warrant careful consideration. The search for novel SGLT2 inhibitors with high affinity remains an ongoing endeavor. Medicine food homology (MFH) herbs show promise for drug development due to their nutritional and medicinal value. Methods: This study aims to address the shortcomings of existing virtual screening models for SGLT2 inhibitors by optimizing feature selection and integrating multidimensional molecular fingerprints. Subsequently, an integrated virtual screening pipeline is constructed to identify potential SGLT2 inhibitors from eight selected MFH herbs. Results: The results indicate that the optimal model (LightGBM and RF) achieved an accuracy of 0.97 and an AUC of 0.98. Following rigorous filtering, a total of 44 potential SGLT2 inhibitors were identified, among which, Isoononin (from Gancao) and Ononin (from Huangqi, Gegen, and Gancao) exhibit favorable drug likeness and safety. Molecular docking demonstrate that both compounds can effectively bind to the SGLT2 active site, establishing stable hydrophobic interactions with critical residues such as Phe98 and Phe453. Furthermore, molecular dynamics simulations confirm the stability of the interactions between the two compounds and SGLT2. Conclusions: This study significantly enhances the accuracy and stability of SGLT2 inhibitor virtual screening models by addressing deficiencies in structural characterization and feature selection. It provides candidate molecules for the development of novel SGLT2 inhibitors and offers new scientific evidence for the application of MFH herbs in the prevention and treatment of chronic metabolic diseases. Full article

Mucor is widely distributed in nature and extensively applied in industries, food, and other fields. Based on analyses of the internal transcribed spacer (ITS) and the large subunit (LSU) of the ribosomal RNA gene, RNA polymerase II largest subunit gene (RPB1), and their morphological characteristics, six new species of Mucor were identified. Mucor catenatus sp. nov. forms chlamydospores in chains. Mucor jujubinus sp. nov. is characterized by jujube-shaped columellae. Mucor macrosporangium sp. nov. produces larger sporangia. Mucor multiramosus sp. nov. features extensively branched sporangiophores. Mucor oligorhizus sp. nov. shows a rare rhizoid formation. Mucor tumidus sp. nov. usually develops swellings in sporangiophores. This study represents the twelfth contribution in a series exploring early-diverging fungal diversity in China and raises the number of accepted Mucor species to 60. Full article

Grain storage is a critical part of preventing food crises and ensuring global food security. The stored grain is susceptible to infestation by borer pests, which not only degrade grain quality but also pose safety risks such as fungal contamination. Consequently, the control of borer pests represents a highly practical research direction in the food field. This review summarizes advances in stored-grain pest control technologies, covering physical, chemical, biological, and green methods. It highlights the advantages of cold plasma for degrading chemical residues via oxidative stress and nanocarriers for stabilizing pesticides, while exploring the development prospects of smart devices in precision control. Significant references are provided for developing new, highly efficient pest control technologies and systems to guarantee grain quality and safety. Full article

The expansion of the consumption of green agricultural products is a vital direction for transforming and upgrading food consumption for China’s residents. The development of the platform economy, based on the development of electronic information technology, achieved through changing business models, economic form, and, especially, the consumption habits and channels of residents, further improves the consumption potential of green agricultural products. This paper uses micro-questionnaire survey data from 424 green agricultural product consumers nationwide and utilizes the propensity score matching (PSM) model to explore the impact of the platform economy on residents’ consumption of green agricultural products. This study found that the platform economy can significantly promote residents’ consumption of green agricultural products. In particular, the positive effect is more significant for urban residents with higher household incomes. In addition, improving residents’ health awareness and value perception is conducive to motivating residents’ green agricultural product consumption. Therefore, this paper proposes suggestions, such as focusing on strengthening the construction of platform infrastructure in rural areas, promoting the development of platform economy in urban areas through innovative application platform technologies, and cultivating residents’ basic literacy for effective participation in the platform economy, to provide new channels and ideas for expanding the consumption of green agricultural products in China. Full article

From adaptation to building effective resilience to climate change is critical for transforming West and Central Africa (WCA) agricultural system. Climate-Smart Agriculture (CSA) is an approach initiated by leading international organizations to ensure food security, increased adaptation to climate change and mitigation. Its application spans from innovative policies, practices, technologies, innovations and financing. However, CSA initiatives lack scientific-based assessment prior to implementation to ensure their effectiveness. To fill this gap, future interventions should not only be assessed using rigorous methodology but should also be built on lessons learned from previous initiatives. Although there are a lot of climate related agricultural initiatives in WCA, most of them have not been analyzed through a CSA lens and criteria to capitalize on their experiences to improve future interventions. In this study we mapped previous climate-related initiatives in WCA, highlighted their gaps and lessons learned to accelerate the implementation of CSA in the region. The study covered 20 countries in WCA: Benin, Burkina Faso, Cameroon, Cape Verde, Central African Republic, Chad, Côte d’Ivoire, Congo, Gabon, Gambia, Ghana, Guinea, Liberia, Mali, Mauritania, Niger, Nigeria, Senegal, Sierra Leone, Togo. CSA initiatives were reviewed using a three-steps methodology: (i) national data collection, (ii) regional validation of the national database, (iii) data analysis including spatial mapping. Data was collected from the websites of international, regional and national organizations working in the field of agricultural development in the region. Each initiative was analyzed using a multicriteria analysis based on CSA principles. A total of 1629 CSA related initiatives were identified in WCA. Over 75% of them were in the form of projects/programs with more of a focus on the first CSA pillar (productivity and food security), followed by adaptation. The mitigation pillar is less covered by the initiatives. Animal production, fisheries, access to markets, and energy are poorly included. More than half of these initiatives have already been completed, calling for more new initiatives in the region. Women benefit very little from the implementation of the identified CSA initiatives, despite the substantial role they play in agriculture. CSA initiatives mainly received funding from technical and financial partners and development partners (45%), banks (22%), and international climate financing mechanisms (20%). Most of them were implemented by government institutions (48%) and development partners (23%). In total, more than 600 billion EUR have been disbursed to implement 83 of the 1629 initiatives identified. These initiatives contributed to reclaiming and/or rehabilitating almost 2 million ha of agricultural land in all countries between 2015 and 2025. Future initiatives should ensure the consideration of the three CSA pillars right from their formulation to the implementation. These initiatives should consider investing in mixed production systems like crop-animal-fisheries. Activities should be built around CSA innovation platforms to encourage networking among actors for more sustainability. Full article