Search Results (2,343)

The persistent challenge of fracture-driven interference (FDI) during large-scale hydraulic fracturing in the southern Sichuan Basin has severely compromised shale gas productivity, while the existing research has inadequately addressed both FDI risk reductions and the optimization of reservoir stimulation. To bridge this gap, this study developed a mechanistic model of the competitive multi-cluster fracture propagation under non-uniform perforation conditions and established a perforation-based design methodology for the mitigation of horizontal well interference. The results demonstrate that spindle-shaped perforations enhance the uniformity of fracture propagation by 20.3% and 35.1% compared to that under uniform and trapezoidal perforations, respectively, with the perforation quantity (48) and diameter (10 mm) identified as the dominant control parameters for balancing multi-cluster growth. Through a systematic evaluation of the fracture communication mechanisms, three distinct inter-well types of FDI were identified: Type I (natural fracture–stress anisotropy synergy), Type II (natural-fracture-dominated), and Type III (stress-anisotropy-dominated). To mitigate these, customized perforation schemes coupled with geometry-optimized fracture layouts were developed. The surveillance data for the offset well show that the pressure interference decreased from 14.95 MPa and 6.23 MPa before its application to 0.7 MPa and 0 MPa, achieving an approximately 95.3% reduction in the pressure interference in the application wells. The expansion morphology of the inter-well fractures confirmed effective fluid redistribution across clusters and containment of the overextension of planar fractures, demonstrating this methodology’s dual capability to enhance the effectiveness of stimulation while resolving FDI challenges in deep shale reservoirs, thereby advancing both productivity and operational sustainability in complex fracturing operations. Full article

Via a One Health approach, this study concomitantly assessed the susceptibility of humans and dogs to Trypanosoma cruzi infections on three islands and in two mainland seashore areas of southern Brazil. Human serum samples were tested using an enzyme-linked immunosorbent assay (ELISA) to detect anti-T. cruzi antibodies, while dog serum samples were tested using indirect fluorescent antibodies in an immunofluorescence assay (IFA). Seropositive human and dog individuals were also tested using quantitative polymerase chain reaction (qPCR) in corresponding blood samples. Overall, 2/304 (0.6%) human and 1/292 dog samples tested seropositive for T. cruzi by ELISA and IFA, respectively, and these cases were also molecularly positive for T. cruzi by qPCR. Although a relatively low positivity rate was observed herein, these cases were likely autochthonous, and the individuals may have been infected as a consequence of isolated events of disturbance in the natural peridomicile areas nearby. Such a disturbance could come in the form of a fire or deforestation event, which can cause stress and parasitemia in wild reservoirs and, consequently, lead to positive triatomines. In conclusion, T. cruzi monitoring should always be conducted in suspicious areas to ensure a Chagas disease-free status over time. Further studies should also consider entomological and wildlife surveillance to fully capture the transmission and spread of T. cruzi on islands and in seashore mainland areas of Brazil and other endemic countries. Full article

Leptospirosis is a globally significant zoonosis affecting animal health, productivity, and the environment. While typically associated with tropical climates, its persistence in semi-arid regions such as La Laguna, Mexico—characterized by low humidity, high temperatures, and limited water sources—remains poorly understood. Although these adverse environmental conditions theoretically limit the survival of Leptospira, high livestock density and synanthropic reservoirs (e.g., rodents) may compensate, facilitating transmission. In this cross-sectional study, blood sera from 445 dairy cows (28 herds: 12 intensive [MI], 16 semi-intensive [MSI] systems) were analyzed via microscopic agglutination testing (MAT) against 10 pathogenic serovars. Urine samples were cultured for active Leptospira detection. Risk factors were assessed through epidemiological surveys and multivariable analysis. This study revealed an overall apparent seroprevalence of 27.0% (95% CI: 22.8–31.1), with significantly higher rates in MSI (54.1%) versus MI (12.2%) herds (p < 0.001) and an estimated true seroprevalence of 56.3% (95% CI: 50.2–62.1) in MSI and 13.1% (95% CI: 8.5–18.7) in MI herds (p < 0.001). The Sejroe serogroup was isolated from urine in both systems, confirming active circulation. In MI herds, rodent presence (OR: 3.6; 95% CI: 1.6–7.9) was identified as a risk factor for Leptospira seropositivity, while first-trimester abortions (OR:10.1; 95% CI: 4.2–24.2) were significantly associated with infection. In MSI herds, risk factors associated with Leptospira seropositivity included co-occurrence with hens (OR: 2.8; 95% CI: 1.5–5.3) and natural breeding (OR: 2.0; 95% CI: 1.1–3.9), whereas mastitis/agalactiae (OR: 2.8; 95% CI: 1.5–5.2) represented a clinical outcome associated with seropositivity. Despite semi-arid conditions, Leptospira maintains transmission in La Laguna, particularly in semi-intensive systems. The coexistence of adapted (Sejroe) and incidental serogroups underscores the need for targeted interventions, such as rodent control in MI systems and poultry management in MSI systems, to mitigate both zoonotic and economic impacts. Full article

In celebration of 50 years of the endoreversible Carnot-like heat engine, this work aims to link the thermodynamic success of the irreversible Carnot-like heat engine with the stability dynamics of the engine. This region of success is defined by two extreme configurations in the interaction between heat reservoirs and the working fluid. The first corresponds to a fully reversible limit, and the second one is the fully dissipative limit; in between both limits, the heat exchange between reservoirs and working fluid produces irreversibilities and entropy generation. The distance between these two extremal configurations is minimized, independently of the chosen metric, in the state where the efficiency is half the Carnot efficiency. This boundary encloses the region where irreversibilities dominate or the reversible behavior dominates (region of success). A general stability dynamics is proposed based on the endoreversible nature of the model and the operation parameter in charge of defining the operation regime. For this purpose, the maximum ecological and maximum Omega regimes are considered. The results show that for single perturbations, the dynamics rapidly directs the system towards the success region, and under random perturbations producing stochastic trajectories, the system remains always in this region. The results are contrasted with the case in which no restitution dynamics exist. It is shown that stability allows the system to depart from the original steady state to other states that enhance the system’s performance, which could favor the evolution and specialization of systems in nature and in artificial devices. Full article

This study investigates ground deformations in the southeastern Po Plain (northern Italy), focusing on the Bologna area—a densely populated region affected by natural and anthropogenic subsidence. Ground deformations in the area result from geological processes (e.g., sediment compaction and tectonic activity) and human activities (e.g., ground water production and underground gas storage—UGS). We apply a multidisciplinary approach integrating subsurface geology, ground water production, advanced differential interferometry synthetic aperture radar—DInSAR, gas storage data, and land use information to characterize and analyze the spatial and temporal variations in vertical ground deformations. Seasonal and trend decomposition using loess (STL) and cluster analysis techniques are applied to historical DInSAR vertical time series, targeting three representatives areas close to the city of Bologna. The main contribution of the study is the attempt to correlate the lateral extension of ground water bodies with seasonal ground deformations and water production data; the results are validated via knowledge of the geological characteristics of the uppermost part of the Po Plain area. Distinct seasonal patterns are identified and correlated with ground water production withdrawal and UGS operations. The results highlight the influence of superficial aquifer characteristics—particularly the geometry, lateral extent, and hydraulic properties of sedimentary bodies—on the ground movements behavior. This case study outlines an effective multidisciplinary approach for subsidence characterization providing critical insights for risk assessment and mitigation strategies, relevant for the future development of CO₂ and hydrogen storage in depleted reservoirs and saline aquifers. Full article

The Shenhu sea area is rich in unconsolidated hydrate reserves, but the formation mineral particles are small, the rock cementation is weak, and the coupling mechanism of hydrate phase change, fluid seepage, and formation deformation is complex, resulting in unclear productivity change law under depressurization exploitation. Therefore, a thermal–fluid–solid–chemical coupling model for natural gas hydrate depressurization exploitation in the Shenhu sea area was constructed to analyze the variation law of reservoir parameters and productivity. The results show that within 0–30 days, rapid near-well pressure drop (13.83→9.8 MPa, 36.37%) drives peak gas production (25,000 m³/d) via hydrate dissociation, with porosity (0.41→0.52) and permeability (75→100 mD) increasing. Within 30–60 days, slower pressure decline (9.8→8.6 MPa, 12.24%) and fines migration cause permeability fluctuations (120→90 mD), reducing gas production to 20,000 m³/d. Within 60–120 days, pressure stabilizes (~7.6 MPa) with residual hydrate saturation < 0.1, leading to stable low permeability (60 mD) and gas production (15,000 m³/d), with cumulative production reaching 2.2 × 10⁶ m³. This study clarifies that productivity is governed by coupled “pressure-driven dissociation–heat limitation–fines migration” mechanisms, providing key insights for optimizing depressurization strategies (e.g., timed heat supplementation, anti-clogging measures) to enhance commercial viability of unconsolidated hydrate reservoirs. Full article

The Three Gorges Reservoir Area (TGRA) is an important ecological barrier and cultural intermingling zone in the upper reaches of the Yangtze River, and its traditional villages carry unique information about natural changes and civilisational development, but face the challenges of conservation and development under the impact of modernisation and ecological pressure. This study takes 112 traditional villages in the TGRA that have been included in the protection list as the research objects, aiming to construct a cultural landscape identification framework for the traditional villages in the TGRA. Through field surveys, landscape feature assessments, GIS spatial analysis, and multi-source data analysis, we systematically analyse their cultural landscape type systems and spatial differentiation characteristics, and then reveal their cultural landscape types and spatial differentiation patterns. (1) The results of the study show that the spatial distribution of traditional villages exhibits significant altitude gradient differentiation—the low-altitude area is dominated by traffic and trade villages, the middle-altitude area is dominated by patriarchal manor villages and mountain farming villages, and the high-altitude area is dominated by ethno-cultural and ecologically dependent villages. (2) Slope and direction analyses further reveal that the gently sloping areas are conducive to the development of commercial and agricultural settlements, while the steeply sloping areas strengthen the function of ethnic and cultural defence. The results indicate that topographic conditions drive the synergistic evolution of the human–land system in traditional villages through the mechanisms of agricultural optimisation, trade networks, cultural defence, and ecological adaptation. The study provides a paradigm of “nature–humanities” interaction analysis for the conservation and development of traditional villages in mountainous areas, which is of practical value in coordinating the construction of ecological barriers and the revitalisation of villages in the reservoir area. Full article

Using common corn stalks as raw materials, a functional dense-structured carbon microsphere with good elastic deformation and certain rigid support was modified from biomass through a step-by-step hydrothermal method. The composition, thermal stability, fluid-loss reduction performance, and reservoir protection performance of the modified carbon microspheres were studied. Research indicates that after hydrothermal treatment, under the multi-level structural action of a small amount of proteins in corn stalks, the naturally occurring cellulose, polysaccharide organic compounds, and part of the ash in the stalks are adsorbed and encapsulated within the long-chain network structure formed by proteins and cellulose. By attaching silicate nanoparticles with certain rigidity from the ash to the relatively stable chair-type structure in cellulose, functional dense-structured carbon microspheres were ultimately prepared. These carbon microspheres could still effectively reduce fluid loss at 200 °C. The permeability recovery value of the cores treated with modified biomass carbon microspheres during flowback reached as high as 88%, which was much higher than that of the biomass itself. With the dense network-like chain structure supplemented by small-molecule aldehydes and silicate ash, the subsequent invasion of drilling fluid was successfully prevented, and a good sealing effect was maintained even under high-temperature and high-pressure conditions. Moreover, since this functional dense-structured carbon microsphere achieved sealing through a physical mechanism, it did not cause damage to the formation, showing a promising application prospect. Full article

This paper presents a numerical simulation study evaluating carbon dioxide capture and storage (CCS) feasibility for the Turceni Power Plant in Oltenia, Romania, using the nearby depleted Bibești-Bulbuceni gas reservoir. A comprehensive reservoir model was developed using Petrel software, integrating geological and reservoir engineering data for the formations of the Bibești-Bulbuceni structure, which is part of the western Moesian Platform. The static model incorporated realistic petrophysical inputs for the Meotian reservoirs. Dynamic simulations were performed using Eclipse compositional simulator with Peng–Robinson equation of state for a CH4-CO₂ system. The model was initialized with natural gas initially in place at 149 bar reservoir pressure, then produced through depletion to 20.85 bar final pressure, achieving 80% recovery factor. CO₂ injection simulations modeled a phased 19-well injection program over 25 years, with individual well constraints of 100 bar bottom-hole pressure and 200,000 Sm³/day injection rates. Results demonstrate successful injection of a 60 Mt CO₂, with final reservoir pressure reaching 101 bar. The modeling framework validates the technical feasibility of transforming Turceni’s power generation into a net-zero process through CCS implementation. Key limitations include simplified geochemical interactions and relying on historical data with associated uncertainties. This study provides quantitative evidence for CCS viability in depleted hydrocarbon reservoirs, supporting industrial decarbonization strategies. The strategy not only aligns with the EU’s climate-neutral policy but also enhances local energy security by repurposing existing geological resources. The findings highlight the potential of CCS to bridge the gap between current energy systems and a sustainable, climate-neutral future. Full article

The establishment and management of nature reserves play a crucial role in protecting biodiversity and supporting sustainable agriculture. This study focuses on 2538 nature reserves in 22 provinces, 5 autonomous regions and 4 municipalities directly under the central government in mainland China. Integrating GIS spatial statistics, imbalance index, and geodetector models, we reveal critical insights: (1) Pronounced spatial inequity is observed, where a small number of eastern provinces dominate the total reserve count, highlighting significant regional disparities in ecological resource allocation. The sparse kernel density in western regions, indicating sparse reserve coverage. The Standard Deviation Ellipse highlights directional dispersion and human-ecological conflicts in high-density zones. (2) Key sustainability indicators driving reserve distribution include: total water resources, water resources per capita, forest area. (3) The spatial distribution of China’s nature reserves, along with factors such as altitude, river distribution, and transportation infrastructure, plays a crucial role in their development. This research provides theoretical support for the scientific planning and policy-making of nature reserves in China and offers practical guidance for optimizing and adjusting sustainable agricultural development. The study emphasizes the vital functions of nature reserves in maintaining ecosystem balance, enhancing regional climate resilience, and serving as biodiversity reservoirs. This research offers strategic insights for integrating nature reserve spatial planning with sustainable agricultural development policies, providing a scientific basis for optimizing the eco-agricultural interface in China. Full article

The inlet operating conditions of centrifugal compressors in natural gas storage reservoirs, as well as the natural gas composition, continuously vary over time, significantly impacting compressor performance. To analyze the influence of these factors on centrifugal compressors, a method for converting the performance curves of centrifugal compressors under actual operating conditions has been established. This performance conversion process is implemented through a custom-developed program, which incorporates the polytropic index and exhaust temperature calculations. Verification results show that the conversion error of this method is within 2%. Based on the proposed performance prediction method for non-similar operating conditions, the effects of varying inlet temperatures, pressures, and natural gas compositions on compressor performance are investigated. It is observed that an increase in inlet temperature results in a decrease in compressor power and pressure ratio; an increase in inlet pressure leads to higher power consumption, while the pressure ratio varies with the flow rate at the operating point; and as the average molar mass of natural gas decreases, both the pressure ratio and power exhibit a certain degree of reduction. Full article

This paper presents a formulation for the dynamic analysis of dam–reservoir–foundation systems, employing a coupled finite element model that integrates displacements and reservoir pressures. An innovative coupled approach, without separating the solid and fluid equations, is proposed to directly solve the single non-symmetrical governing equation for the whole system with non-proportional damping. For the modal analysis, a state–space method is adopted to solve the coupled eigenproblem, and complex eigenvalues and eigenvectors are computed, corresponding to non-stationary vibration modes. For the seismic analysis, a time-stepping method is applied to the coupled dynamic equation, and the stress–transfer method is introduced to simulate the nonlinear behavior, innovatively combining a constitutive joint model and a concrete damage model with softening and two independent scalar damage variables (tension and compression). This formulation is implemented in the computer program DamDySSA5.0, developed by the authors. To validate the formulation, this paper provides the experimental and numerical results in the case of the Cahora Bassa dam, instrumented in 2010 with a continuous vibration monitoring system designed by the authors. The good comparison achieved between the monitoring data and the dam–reservoir–foundation model shows that the formulation is suitable for simulating the modal response (natural frequencies and mode shapes) for different reservoir water levels and the seismic response under low-intensity earthquakes, using accelerograms measured at the dam base as input. Additionally, the dam’s nonlinear seismic response is simulated under an artificial accelerogram of increasing intensity, showing the structural effects due to vertical joint movements (release of arch tensions near the crest) and the concrete damage evolution. Full article

Natural products, characterized by their structural novelty, multi-target capabilities, and favorable toxicity profiles, represent a prominent reservoir for the discovery of innovative anticancer therapeutics. In the current investigation, we identified ajuforrestin A, a diterpenoid compound extracted from Ajuga lupulina Maxim, as a potent agent against lung cancer. In vitro, this compound markedly curtailed the proliferation of A549 cells. Mechanistic explorations revealed that ajuforrestin A could arrest A549 cells in the G0/G1 phase of the cell cycle, provoke apoptosis in cancer cells, and impede their migration by modulating the STAT3 and FAK signaling cascades. Angiogenesis is indispensable for tumor formation, progression, and metastatic dissemination. Vascular endothelial growth factor (VEGF) and its receptor VEGFR-2 are established as crucial mediators in tumor neovascularization, a process fundamental to both the expansion of tumor cells and the development of new blood vessels within the tumor milieu. Through the combined application of a Tg(fli1:EGFP) zebrafish model and SPR experimentation, we furnished strong evidence for the ability of ajuforrestin A to obstruct tumor angiogenesis via selective engagement with VEGFR-2. Finally, a zebrafish xenograft tumor model demonstrated that ajuforrestin A could effectively restrain tumor growth and metastasis in vivo. Ajuforrestin A therefore shows considerable promise as a lead compound for the future development of therapies against non-small cell lung cancer (NSCLC). Full article

Peptides play a crucial role in the development of pharmaceuticals and functional foods. Multiple studies have shown that natural bioactive peptides possess antioxidant, antihypertensive, anti-tumor, and anti-inflammatory activities. Marine bioactive peptides, especially those sourced from fish, constitute a substantial reservoir of these molecules. Although considerable research has been undertaken on fish-derived peptides, studies specifically concerning those from tuna are limited. Tuna, a marine fish of high nutritional value, generates substantial by-product waste during fishing and processing. Therefore, it is essential to conduct an evaluation of the advancements in study on tuna-derived active peptides and to offer a perspective on the direction of future investigations. This review integrates prospective bioactive peptides derived from tuna and reports contemporary strategies for their investigation, including extraction, purification, screening, identification, and activity evaluation procedures, including Yeast Surface Display (YSD) and molecular docking. This review seeks to promote the continued investigation and application of bioactive peptides derived from tuna. Full article

Nipah virus (NiV) and Hendra virus (HeV), which both belong to the genus henipavirus, are zoonotic pathogens that cause severe systemic, neurological, and/or respiratory disease in humans and a variety of mammals. Therefore, monitoring viral prevalence in natural reservoirs and rapidly diagnosing cases of henipavirus infection are critical to limiting the spread of these viruses. Current laboratory methods for detecting NiV and HeV include virus isolation, reverse transcription quantitative real-time PCR (RT-qPCR), and antigen detection via an enzyme-linked immunosorbent assay (ELISA), all of which require highly trained personnel and specialized equipment. Here, we describe the development of a point-of-care customized immunochromatographic lateral flow (ILF) assay that uses recombinant human ephrin B2 as a capture ligand on the test line and a NiV-specific monoclonal antibody (mAb) on the conjugate pad to detect NiV and HeV. The ILF assay detects NiV and HeV with a diagnostic specificity of 94.4% and has no cross-reactivity with other viruses. This rapid test may be suitable for field testing and in countries with limited laboratory resources. Full article