Search Results (51)

Non-communicable diseases (NCDs) such as cardiovascular disease, diabetes, cancer, and chronic respiratory conditions are the leading causes of death globally, largely driven by modifiable lifestyle factors. With growing interest in dietary strategies for NCDs prevention and management, functional foods like watercress (Nasturtium officinale) have attracted attention for their rich phytochemical content and potential health benefits. This narrative review synthesizes 88 sources published between 2019 and March 2025, exploring the effects of watercress bioactive compounds on major NCDs. Watercress is abundant in glucosinolates, isothiocyanates (especially phenethyl isothiocyanate), flavonoids, vitamins, and minerals. These compounds contribute to antioxidant, anti-inflammatory, and metabolic regulatory effects. Preclinical and clinical studies show that watercress supplementation may improve lipid profiles, reduce oxidative stress, and modulate inflammation in cardiovascular and respiratory conditions. It also appears to enhance insulin function and reduce blood glucose levels. In cancer models, watercress extracts exhibit antiproliferative, pro-apoptotic, and chemoprotective properties, with selective toxicity towards cancer cells and protective effects on normal cells. These findings highlight the therapeutic potential of watercress as a dietary adjunct in NCDs prevention and management, supporting the need for further clinical research. Full article

The garden Nasturtium (Tropaeolum majus L.) is increasingly consumed worldwide due to its culinary appeal and perceived health benefits. However, the chemical markers underlying its functional properties remain insufficiently characterized. Building on evidence from a recent human pilot study confirming both high acceptability and dietary safety, we conducted a comprehensive volatilomic and phytochemical analysis of T. majus flowers and their juice. Headspace solid-phase microextraction coupled with gas chromatography–mass spectrometry (HS-SPME/GC-MS) was employed to establish the volatilomic fingerprint of floral tissues and juice. Our analysis revealed a striking dominance of benzyl isothiocyanate and benzonitrile, which together accounted for 88% of the total volatile organic metabolites (VOMs) in the juice, 67% and 21%, respectively. In the floral tissues, benzyl isothiocyanate was even more prevalent, representing 95% of the total volatile profile. Complementary in vitro assays confirmed a substantial total phenolic content and strong antioxidant activity in the flowers. These findings provide a robust chemical rationale for the potential health-promoting attributes of T. majus, while identifying key volatilomic markers that could support future functional and safety claims. In parallel, a benefit–risk assessment framework is discussed in accordance with the European Food Safety Authority (EFSA) guidelines for the Qualified Presumption of Safety (QPS) of edible flowers. Given that both benzyl isothiocyanate and benzonitrile are classified as Cramer Class III substances, a conservative intake threshold of 1.5 μg/kg body weight per day is proposed. To enable quantitative exposure modeling and support the derivation of a tolerable daily intake (TDI), future studies should integrate organic solvent-based extraction methodologies to estimate the total volatile load per gram of floral biomass. This would align risk–benefit assessments with the EFSA’s evolving framework for novel foods and functional ingredients. Full article

This study evaluates the potential water availability in Barka Slough and the effects of changing hydrological conditions on the aquatic habitat of five protected species. Barka Slough is a historically perennial wetland at the downstream western end of the San Antonio Creek Valley watershed (SACVW). A previously published hydrologic model of the SACVW for 1948–2018 was extended to include 2019–2021 and then modified to simulate the future years of 2022–2051. Two models simulating the future years of 2022–2051 were constructed, each with different climate inputs: (1) a repeated historical climate and (2) a 2070-centered Drier Extreme Warming climate (2070 DEW). The model with the 2070 DEW climate had warmer temperatures and an increase in average annual precipitation driven by larger, albeit more infrequent, precipitation events than the model with the historical climate. Simulated groundwater pumpage resulted in cumulative groundwater storage depletion and groundwater-level decline in Barka Slough in both future models. The simulations indicate that Barka Slough may transition from a perennial to an ephemeral wetland. Streamflow, stream disconnection, and depth to groundwater are key habitat metrics for federally listed species in Barka Slough. Future seasonal conditions for each metric are more likely to affect federally listed species’ habitats under 2070 DEW climatic conditions. Future seasonal streamflow volume may negatively impact unarmored threespine stickleback (Gasterosteus aculeatus williamsoni) and tidewater goby (Eucyclogobis newberryi) habitats. Future seasonal stream disconnection may negatively impact the unarmored threespine stickleback habitat. Future groundwater-level decline may negatively impact Gambel’s watercress (Nasturtium gambelii) and La Graciosa thistle (Cirsium scariosum var. loncholepis) habitats and could influence the ability to use Barka Slough as a restoration or reintroduction site for these species. Results from this study can be used to inform water management decisions to sustain future groundwater availability in the SACVW. Full article

Salinity represents a significant challenge for agriculture, especially in semi-arid regions, affecting the growth and productivity of plants such as nasturtium (Tropaeolum majus), which is valued for its ornamental, medicinal, and food uses. Salt stress disrupts biochemical, physiological, and anatomical processes, limiting plant development. This study investigated the application of attenuators, including salicylic acid, nicotinamide, and proline, to mitigate the effects of salt stress on nasturtium cultivated in a hydroponic system. The treatments involved different combinations of these compounds under saline conditions (40 mM NaCl). The attenuators reduced the negative impacts of salt stress, promoting improvements in gas exchange, such as increased net photosynthesis, water-use efficiency, and stomatal conductance. Additionally, the treatments enhanced vegetative and reproductive growth, increasing the dry biomass of leaves, stems, and flowers, as well as the number of flowers and flower buds. The combination of salicylic acid, nicotinamide, and proline stood out by providing greater efficiency in carbon assimilation, stability of photosynthetic pigments, and higher tolerance to salt stress. These findings reinforce the potential of using attenuators to optimize the cultivation of nasturtium in saline environments, promoting higher productivity and plant quality. Full article

Enterococcus faecalis is strongly associated with secondary/persistent root canal infections, being the most prevalent bacterium in cases of apical periodontitis in previously treated teeth. This study was elaborated to evaluate the anti-planktonic, antibiofilm, and synergistic effects of Nasturtium officinale and Psidium guajava hydroethanolic extracts against standard and clinical strains of E. faecalis. Firstly, the N. officinale extract was prepared from watercress leaves, and P. guajava extract was prepared from guava tree leaf shoots. Then, the content of soluble solids was quantified in both. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of the isolated N. officinale and P. guajava extracts for each bacterial strain were determined using the broth microdilution method, following the Clinical and Laboratory Standards Institute (CLSI) guideline M7-A9. The MTT assay was used to evaluate the antibiofilm activity, and the fractional bactericidal concentration index (FBCI) was utilized to evaluate the synergistic effect of the N. officinale and P. guajava extracts using the checkerboard technique. Again, the MTT assay was used to evaluate the antibiofilm activity of the combined extracts this time. The data were subjected to statistical analysis using ANOVA and Tukey’s test, with a significance level of p ≤ 0.05. It was found that the soluble solid content of N. officinale was 50 mg/mL, and of P. guajava was 33.5 mg/mL. The MBC value of N. officinale was 12.5 mg/mL, and of P. guajava was 0.52 mg/mL against all the tested strains of E. faecalis. The combined 0.1 mg/mL N. officinale + 0.1 mg/mL P. guajava, and 0.1 mg/mL N. officinale + 0.5 mg/mL P. guajava hydroethanolic extracts effectively reduced the biofilm formation of the standard and clinical strain 4 of E. faecalis. Therefore, these combined extracts may be considered as endodontic irrigants in future studies. Full article

Nasturtium officinale R. Br. (watercress) is an endangered species with valuable pharmaceutical, cosmetic, and nutritional properties. The purpose of this work was to evaluate the phytochemical profile and biological activity of extracts from microshoot cultures grown in PlantForm bioreactors and the parent plant material. After 20 days of cultivation, the cultures achieved the best results both in terms of key active ingredient content and biological activity. The glucosinolates (GSL) profile by the UHPLC-DAD-MS/MS method showed that the dominant compounds were glucobrassicin (493.00 mg/100 g DW, 10 days) and gluconasturtiin (268.04 mg/100 g DW, 20 days). The highest total polyphenol content (TPC) was obtained after a 20-day growth period (2690 mg GAE/100 g DW). Among polyphenols, the dominant compounds in the extracts from in vitro cultures were sinapinic acid (114.83 mg/100 g DW, 10 days) and ferulic acid (87.78 mg/100 g DW, 20 days). The highest antioxidant potential assessed by ABTS and DPPH assays was observed for ethanol extracts. The best results for inhibiting hyperpigmentation (18.12%) were obtained for ethanol extracts and anti-elastase activity (79.78%) for aqueous extract from N. officinale microshoot cultures. The extracts from microshoot cultures inhibited the growth of bacteria, including Cutibacterium acnes (MIC = 0.625 mg/mL). Antioxidant tests and the chelating capacity of iron ions Fe²⁺ of the face emulsion with N. officinale extracts showed higher results than the control. Full article

Salinity is a significant challenge for agriculture in semi-arid regions, affecting the growth and productivity of plants like Tropaeolum majus (nasturtium), which is valued for its ornamental, medicinal, and food uses. Salt stress disrupts the plant’s biochemical, physiological, and anatomical processes, limiting its development. This study investigates the potential of proline as an osmoprotectant to mitigate the effects of salt stress on nasturtium’s growth and physiology. A completely randomized factorial design was employed, testing five levels of electrical conductivity (0.0, 1.50, 3.00, 4.5, 6.5 dS m⁻¹) and four proline concentrations (0.0, 5.00, 10.0, 15.0 mM) with six replicates. The results showed that proline application, particularly at 15.0 mM, enhanced growth parameters such as leaf number, stem diameter, and root length. At moderate salinity (3.0 dS m⁻¹), proline significantly improved gas exchange, increasing net photosynthesis, transpiration, and stomatal conductance. Additionally, proline reduced the negative impact of salt stress on the fresh mass of leaves, stems, and roots, and increased both the mass and number of flowers. Proline also elevated the levels of total phenolic compounds and vitamin C while reducing soluble sugars, particularly under moderate salt stress (4.75 dS m⁻¹). Overall, applying 15.0 mM proline shows promise for enhancing the biomass accumulation, flower production, and overall quality of nasturtium under saline conditions. Full article

Watercress (Nasturtium officinale), a freshwater aquatic plant in the Brassicaceae family, is characterized by its high content of specialized metabolites, including flavonoids, glucosinolates, and isothiocyanates. Traditionally, commercial cultivation is conducted in submerged beds using river or spring water, often on soil or gravel substrates. However, these methods have significant environmental impacts, such as promoting eutrophication due to excessive fertilizer use and contaminating water sources with pesticides. This study aimed to explore two emerging cultivation strategies, i.e., hydroponics and aquaponics, to grow watercress and evaluate its specialized metabolite content using an untargeted metabolomic approach. The goal was to characterize metabolic profiles, identify component variations, and assess changes in metabolite accumulation at two harvest times. Two culture systems (hydroponic and aquaponic) and two harvest stages (‘baby leaf’ and traditional harvest) were examined. The results revealed 23 key metabolites, predominantly glucosinolates and flavonoids, that significantly influenced the metabolic profile discrimination, with the aquaponic system yielding the highest diversity and relative abundance of metabolites (variable importance in the projection (VIP) > 1). Important condition-related compounds were identified via cross-validation (area under the curve (AUC) > 0.7), including isorhamnetin sophoroside–glucoside and gluconasturtiin at the traditional harvest in the hydroponic system and glucoarabin at the ‘baby leaf’ stage in the aquaponic system. These findings highlight the potential of aquaponic and hydroponic systems as sustainable alternatives for watercress cultivation, offering environmental benefits and enhanced metabolite quality. Full article

Light spectrum and intensity is one of the key factors in the production of microgreens in controlled-environment agriculture and is directly related to plant growth and biomass accumulation. Hence, the objective of this research study was to investigate the biomass, growth, and resource use efficiencies (RUEs) in 14 different species of microgreen grown in two light recipes with 209.5 (OSRAM LED) and 45 µmol m⁻² s⁻¹ (INSTAGREEN LED) with a 16/8 h light/dark photoperiod in a growth chamber. Under both LEDs, fresh biomass accumulation and the SPAD content were highest in sunflower. Nasturtium recorded the maximum hypocotyl length under both LEDs. The leaf area index (LAI) was significantly higher in mungbean under the INSTAGREEN LED compared to other microgreens, while the maximum LAI was measured in lentils under the OSRAM LED. This shows that the two different LEDs had species-specific effects. The RUE of the cheaper INSTAGREEN LED was more efficient in terms of light and energy use efficiency, while OSRAM LED was more efficient in terms of water and surface use efficiencies. Overall, the results showed that different species of microgreens exhibit different responses to fresh biomass accumulation and SPAD contents in the leaves, demonstrating the diversity of their growth responses. Across both LEDs (OSRAM LED and INSTAGREEN LED), the top performing microgreen in terms of biomass accumulation as well as SPAD contents in the leaves was sunflower. Consequently, a high chlorophyll content in sunflower led to a higher biomass production by enhancing photosynthesis. Full article

Increasing research is being directed toward the production of value-added products using plant extracts that are super-fortified with antioxidants. In this study, the extraction parameters for bioactive compounds (such as polyphenols) from Nasturtium officinale leaves and their antioxidant properties were optimized using response surface methodology. The optimization procedure examined the effects of the extraction temperature, time, and solvent composition on conventional magnetic stirring (ST). In addition, the impacts of two green techniques—pulsed electric field (PEF) and ultrasound (US)—were evaluated individually and in combination to assess their potential to enhance the extraction of the compounds. According to our findings, under the proposed extraction conditions (a combination of PEF, US, and ST as a extraction technique, 50% ethanolic solvent, for 30 min at 80 °C). N. officinale leaf extract proved to be an excellent source of bioactive compounds, with extracts containing rosmarinic acid (3.42 mg/g dried weight (dw)), chlorogenic acid (3.13 mg/g dw), total polyphenol content (28.82 mg of gallic acid equivalents (GAE)/g dw), and strong antioxidant properties. The FRAP method measured 57.15 μmol ascorbic acid equivalents (AAE)/g dw, while the DPPH radical scavenging activity method measured 47.55 μmol AAE/g dw. This study was carried out to evaluate and improve the concentration of bioactive compounds in N. officinale leaf extract, resulting in a product with multiple applications across the food, cosmetic, and pharmaceutical industries. Full article

Watercress (Nasturtium officinale R.Br.) is a cruciferous aquatic vegetable that possesses significant nutritional value. The NAC family is a transcription factor family specific to plants that play an important role in regulating plant responses to abiotic stress. In order to investigate the response of NAC genes to flooding stress in watercress, we conducted a study on the NoNAC family. In this study, a total of 119 NoNAC genes were obtained through genome-wide identification. Phylogenetic analysis indicated that the NoNAC family members can be categorized into ten subgroups. The results of gene structure analysis revealed that each branch within the subgroups exhibited similar motif composition and gene structure. The heatmap analysis showed that several NoNAC genes demonstrated tissue-specific expression patterns, suggesting their potential as regulators of associated tissue development. As an aquatic plant, watercress serves as a valuable material for investigating plant resistance to flooding stress. This study found that flooding can significantly increase the watercress plant height, which is a typical escape strategy under flooding. The analysis of the expression of NoNAC genes in the stem transcriptome after flooding indicated that only NoNAC36a consistently exhibited significant differential changes and down-regulated expression at the three time points of flooding treatment. This suggests that NoNAC36a may be involved in regulating watercress plant height increases under flooding stress. The utilization of a virus-induced gene silencing assay to investigate the biological function of NoNAC36a revealed that NoNAC36a silencing caused cell elongation and expansion, thus increasing watercress plant height. The yeast one-hybrid and dual luciferase assays demonstrated that NoNAC36a binds the promoter of NoXTH33 and inhibits its expression. Subsequently, the results of yeast two-hybrid, luciferase complementary, and pull-down assays revealed the interaction between NoMOB1A and NoNAC36a in vivo and in vitro. Sequence alignment indicated that NoMOB1A and AtMOB1A share an identical amino acid sequence. RT-qPCR analysis indicated that flooding prompted the expression of NoMOB1A in stems. Thus, it is speculated that NoMOB1A may exhibit functions similar to AtMOB1A and that the up-regulation of NoMOB1A expression in stems may facilitate an increase in plant height under flooding. In summary, the NoNAC family was analyzed, and revealed a regulatory network centered on NoNAC36a that facilitates watercress resistance to flooding stress. This study enhanced the understanding of the NoNAC genes and established a theoretical foundation for investigating plant flooding tolerance. Full article

Background: The goal of this study was to evaluate whether the medical recommendation of Angocin^® Anti-Infekt N, compared to standard antibiotic treatment shortly after the diagnosis of a urinary tract infection (UTI) or cystitis, is negatively associated with an early, sporadic, or recurrent UTI, subsequent antibiotic prescriptions, pyelonephritis as a renal complication, or UTI-associated sick leave. Methods: This retrospective cohort study was based on data from the IQVIA^TM Disease Analyzer database and included patients diagnosed with acute UTI or cystitis by physicians in Germany between 2005 and 2021, who were prescribed either Angocin^® or standard antibiotics within 4 days after diagnosis. Patients prescribed antibiotics were matched to those prescribed Angocin^® (5:1) using propensity scores. Univariable logistic and Cox regression models were used to investigate the association between Angocin^® prescription and the defined study outcomes. The effects of Angocin^® were adjusted for age, sex, insurance status, index diagnosis, and physician specialty. Results: A total of 2277 Angocin^® patients and 11,385 antibiotic patients were available for analysis. Compared to antibiotic prescriptions, Angocin^® prescription was associated with significantly lower odds of an early relapse within 1–30 days after the index date (odds ratio (OR): 0.74; 95% confidence interval (CI): 0.62–0.87; p < 0.001), further sporadic UTI within 31–365 days after the index date (OR: 0.68; 95% CI: 0.58–0.78; p < 0.001), and recurrent UTI (OR: 0.63; 95% CI: 0.48–0.82; p < 0.001). This was also accompanied by reduced antibiotic prescriptions (1–30 days: OR: 0.63; 95% CI: 0.53–0.74, p < 0.001; 31–365 days: OR: 0.56; 95% CI: 0.49–0.64, p < 0.001). A strong, but due to the low incidence, not significant, negative association was observed between Angocin^® prescription and the occurrence of pyelonephritis (hazard ratio (HR): 0.67; 95% CI: 0.43–1.06; p = 0.073). Conclusions: The results of this real-world data study demonstrate that Angocin^® can be an effective therapeutic option for managing acute and recurrent UTIs and serves as an alternative therapy to antibiotics. Full article

Edible flowers are becoming increasingly popular as food products, since they give aroma, color, and visual appeal and are also health-promoting compounds. However, they are a highly perishable product, thus post-harvest technologies are needed to extend their marketability. In this study, active (N₂: 100%) and passive modified atmosphere packaging (MAP) technologies were applied to three edible flower species, namely Begonia grandiflora ‘Viking’, Tropaeolum majus, and Viola cornuta, stored at 4 °C. Even if the flowers’ quality decay occurred differently according to the species, active MAP better maintained petal colors and slowed down the edible flowers’ decay than passive MAP by decreasing flower respiration in all three species and sugars consumption in begonia; there was weight loss in nasturtium, and better preserved total phenolic content in begonia and viola. Coupling cold storage with active MAP can be an effective method to extend edible flowers’ post-harvest life. Full article

Nasturtium officinale R.Br. (Betong watercress) contains high levels of secondary metabolites that offer health benefits. However, fresh-cut watercress has a short shelf life. This study aimed to assess the effect of drying methods on the phytochemical contents, antioxidant activity, and anti-diabetic activity of Betong watercress. The watercress was dried using three methods: roasting (R, 50 °C, 40 min); roasting and drying (RD, 40 min roasting at 50 °C and 1 h drying at 80 °C); and blanching, roasting, and drying (BRD, 30 s blanching at 80 °C, 20 min roasting at 50 °C, and 1 h drying at 80 °C). Aqueous extracts from each drying method were analyzed for total phenolic content, total flavonoid content, total glucosinolate content, antioxidant activities (FRAP, DPPH, and ABTS assays), and α-amylase enzyme inhibition. From the results, the R method provided the highest level of total phenolic, total flavonoid, and total glucosionolate content compared to the RD and BRD methods. Similarly, antioxidant activities and α-amylase enzyme inhibition were highest in the R method, followed by the RD and BRD methods. Our results demonstrate that roasting of Betong watercress without the addition of blanching or drying effectively preserves the phytochemical contents, antioxidant activities, and anti-diabetic activity. Full article