Search Results (74)

Small and Medium-sized Enterprises (SMEs) are central to the industrial fabric of Sub-Saharan Africa (SSA). Yet, they confront increasing demands to implement sustainability practices originating from institutional contexts markedly different from their own. Existing research has tended to neglect the cultural and institutional negotiations inherent in this process, often framing sustainability adoption as a technical or compliance-oriented exercise rather than as a multifaceted cultural adaptation. This study proposes and empirically examines the concept of sustainability acculturation—the process by which firms align global sustainability norms with local business cultures. Drawing on Institutional Theory, the Resource-Based View, and Berry’s Acculturation Model, we present a context-specific framework, tested using a sequential explanatory mixed-methods approach: survey data from 284 manufacturing SMEs across six SSA countries, followed by 24 semi-structured interviews. Structural equation modeling reveals that international market pressure and owner–manager values are direct drivers, whereas local regulatory pressure exhibits only a weak association with deep cultural integration. Managerial commitment and organizational learning mediate these relationships, while Ubuntu values enhance social sustainability integration, and institutional voids diminish regulatory effectiveness. The model accounts for 57% of the variance in sustainability acculturation. Findings show that SSA SMEs employ distinct acculturation strategies—Integration, Assimilation, Resilient Adaptation, and Decoupling—shaped by the interplay of external pressures, internal capabilities, and contextual conditions. The study underscores the importance of culturally attuned, context-specific interventions for sustainable industrial development in SSA. Full article

The current debate on digital education is at a crossroads between the need for technological innovation and the growing concern about the impact of passive screen use. In this context, identifying sustainable pedagogical models that integrate Information and Communication Technologies (ICT) in a meaningful and inclusive way is an urgent need. This article presents a case study of the Amara Berri System (ABS), aiming to analyze how inclusive and sustainable digital innovation is operationalized within the system and whether teachers’ length of service is associated with the implementation and perceived impact of inclusive ICT practices. The investigation is based on a mixed-methods sequential design. A questionnaire was administered to a sample of 292 teachers to collect data on their practices and perceptions. Subsequently, a focus group with eight teachers was conducted to further explore the meaning of their practices. Quantitative results show that the implementation and positive evaluation of inclusive ICT practices correlate significantly with teachers’ seniority within the system, which suggests that the model is formative in itself. Qualitative analysis shows that ICTs are not an end in themselves within the ABS, but an empowering tool for the students. The “Audiovisual Media Room”, managed by students, functions as a space for social and creative production that gives technology a pedagogical purpose. The study concludes that the sustainability of digital innovation requires coherence with the pedagogical project. Findings offer valuable implications for the design of teacher training contexts that foster the integration of technology within a framework of truly inclusive education. Full article

Mixed dementia, most often caused by the coexistence of Alzheimer’s disease and vascular dementia pathologies, presents unique preventive and therapeutic challenges that may be addressed through dietary and nutraceutical interventions. Current evidence demonstrates that diets emphasizing polyphenol-rich foods like olive oil, berries, and leafy greens exert neuroprotective effects by reducing oxidative stress, inflammation, and amyloid pathology while improving cerebrovascular function. Specific bioactive compounds, including resveratrol, curcumin, quercetin, epigallocatechin gallate, N-acetylcysteine, and Huperzine A, among some others, have demonstrated therapeutic potential through their multimodal mechanisms targeting the pathogenic pathways of Alzheimer’s disease and vascular dementia, including Aβ and tau pathology, neuroinflammation, oxidative stress, and neurovascular dysfunction. However, our limited appreciation of the pharmacokinetics and pharmacodynamics of natural compounds and the inadequate extent of clinical studies underscore the need for further research. This review synthesizes current knowledge on diet and nutraceutical compounds that may be of value in the prevention and treatment of mixed Alzheimer’s disease and vascular dementia. We focus on their molecular mechanisms of action relevant to the dual pathophysiological basis of mixed Alzheimer’s disease and vascular dementia. Full article

Pollen identification is critical for melissopalynology (honey authentication), ecological monitoring, and allergen tracking, yet manual microscopic analysis remains labor-intensive, subjective, and error-prone when multiple grains overlap in realistic samples. Existing automated approaches often fail to address multi-grain scenarios or lack systematic comparison across classical and deep learning paradigms, limiting their practical deployment. This study proposes a subinstance-based classification framework combining YOLOv12n object detection for grain isolation, independent classification via classical machine learning (ML), convolutional neural networks (CNNs), or Vision Transformers (ViTs), and majority voting aggregation. Five classical classifiers with systematic feature selection, three CNN architectures (ResNet50, EfficientNet-B0, ConvNeXt-Tiny), and three ViT variants (ViT-B/16, ViT-B/32, ViT-L/16) are evaluated on four datasets (full images vs. isolated grains; raw vs. CLAHE-preprocessed) for four berry pollen species (Ribes nigrum, Ribes uva-crispa, Lonicera caerulea, and Amelanchier alnifolia). Stratified image-level splits ensure no data leakage, and explainable AI techniques (SHAP, Grad-CAM++, and gradient saliency) validate biological interpretability across all paradigms. Results demonstrate that grain isolation substantially improves classical ML performance (F1 from 0.83 to 0.91 on full images to 0.96–0.99 on isolated grains, +8–13 percentage points), while deep learning excels on both levels (CNNs: F1 = 1.000 on full images with CLAHE; ViTs: F1 = 0.99). At the instance level, all paradigms converge to near-perfect discrimination (F1 ≥ 0.96), indicating sufficient capture of morphological information. Majority voting aggregation provides +3–5% gains for classical methods but only +0.3–4.8% for deep models already near saturation. Explainable AI analysis confirms that models rely on biologically meaningful cues: blue channel moments and texture features for classical ML (SHAP), grain boundaries and exine ornamentation for CNNs (Grad-CAM++), and distributed attention across grain structures for ViTs (gradient saliency). Qualitative validation on 211 mixed-pollen images confirms robust generalization to realistic multi-species samples. The proposed framework (YOLOv12n + SVC/ResNet50 + majority voting) is practical for deployment in honey authentication, ecological surveys, and fine-grained biological image analysis. Full article

Background/Objectives: The study investigated the potential of mixed fruits and berries (MFB) as a dietary intervention to mitigate cafeteria (CAF) diet-induced gut microbiome dysbiosis and hepatic dysfunction associated with metabolic syndrome and steatohepatitis (MASH) in an adolescent rat model. Methods: Forty-eight adolescent male Sprague-Dawley rats (n = 3 cages per group (two rats per cage)) were divided into eight experimental groups, where NC received the normal AIN-93G basal diet, PC received the CAF diet and normal AIN-93G basal diet, T₁ and T₂ received MFB supplementation (3% and 6% levels) without CAF exposure, P₁ and P₂ received a MFB (3% and 6% levels) supplementation initiated at the onset of CAF feeding, and I₁ and I₂ received MFB supplementation initiated 2 weeks after CAF feeding. After 6 weeks, cecal 16S rRNA, hepatic histopathology, Oil Red O staining, and metabolic dysfunction-associated steatotic liver disease (MASLD)-related biomarkers (liver enzymes, alanine aminotransferase (ALT), and aspartate aminotransferase (AST)) were analyzed. Results: AST: ALT ratio was the highest in the PC group (3.63, p < 0.05) compared to the MFB groups. Oil Red O staining showed lower hepatic lipid accumulation, and histological analysis demonstrated a marked reduction in portal inflammatory cell infiltration in MFB. Alpha diversity (Simpson Index) decreased in PC (Kruskal–Wallis, p = 0.043). CAF increased Lactobacillus johnsonii (+75%, p < 0.05), while reducing L. murinus and L. intestinalis (~90%, p < 0.05). MFB supplementation restored Bifidobacterium Pseudolongum and increased Akkermansia muciniphila levels in the P₂, I₁, and I₂ groups (~20-fold, p < 0.05). Bacteroides dorei was present in all groups except the PC group. These bacteria presented a positive correlation with key SCFAs. Conclusions: The results from this study indicated that MFB supplementation modulated gut microbiota composition and enhanced SCFA production, thereby strengthening intestinal barrier integrity and reducing gut-derived inflammation. Collectively, these effects attenuated hepatic lipid accumulation and inflammation, highlighting the potential of MFB to restore gut–liver axis homeostasis disrupted by CAF-induced dysbiosis in adolescent rats. Full article

Chronic stress impairs cognition through gut–brain axis dysregulation and metabolic imbalance. This study applied untargeted fecal metabolomics to investigate the cognitive and metabolic effects of Bacopa monnieri (L.) Wettst (Brahmi), mixed Thai berry, and their combined extracts in rats exposed to chronic unpredictable mild stress. Cognitive performance was evaluated using the novel object recognition test. Fecal metabolites were profiled using LC-ESI-QTOF-MS, followed by data preprocessing and multivariate statistical analysis. Orthogonal partial least squares regression was applied to identify metabolites associated with the recognition index, and pathway enrichment analysis was subsequently performed to interpret biological relevance. All interventions were associated with improved recognition performance and treatment-related metabolic modulation. Biosynthesis of unsaturated fatty acids was consistently enriched across treatment groups, indicating a shared involvement of lipid remodeling. Treatment-specific responses were also observed: Brahmi was associated with linoleic and alpha-linolenic acid metabolism; mixed Thai berry extract demonstrated dose-dependent modulation of lipid metabolism, with low-dose supplementation additionally yielding amino-acid-derived metabolites; and bile acid-related signaling was uniquely detected in the low-dose combined extract group. These findings demonstrate that fecal metabolomics can capture distinct metabolic signatures associated with herbal extract-induced cognitive improvement and highlight lipid remodeling as a shared metabolic feature across interventions under chronic stress. Full article

This study establishes explicit Berry–Esseen bounds for residual kernel density estimators in AR(1) models with $\alpha$-mixing errors. Since the true innovations are unobservable, we introduce a residual-based estimator ${\widehat{f}}_n\left(x\right)$ and establish its normal approximation under stationarity. By imposing conditions on the bandwidth, mixing coefficients, and moments, we obtain Kolmogorov distance bounds between the standardized estimator and its Gaussian limit. These bounds explicitly depend on the bandwidth, block parameters, and mixing coefficients. A key corollary quantifies the convergence rate as $O\left(n^{(2c-2b+a)/4}\right)$. Our results generalize prior work, advancing theoretical foundations for nonparametric inference in high-dimensional time series. Full article

Grape harvest time influences the berry composition, which impacts the organoleptic wine characteristics. Winemakers monitor technological, phenolic, and aromatic grape maturity to decide the harvest date. Little is known about the evolution of yeast ecology of grapes during the maturation period. The microbiota involved in the fermentation impacts the wine composition and characteristics; therefore, changes in grape biodiversity could have an impact in fermentation kinetics and aroma compound formation. In this study, the yeast biodiversity of Grenache Noir and Carignan grapes from Montsant DO (Denomination of Origin) were analyzed at different ripening stages to assess how harvest time influences microbiota. The fermentation performance of the yeasts obtained was studied at both laboratory and pilot scales to evaluate the impact of these yeasts, both in pure and mixed cultures, on the wine’s chemical and aromatic composition as well as its sensory impact. The results indicated that early harvest favored a higher diversity of non-Saccharomyces species, whereas in more mature grapes, Saccharomyces cerevisiae species was dominant. The isolated strains of Saccharomyces differed in their fermentation performances, as well as ethanol content and acidity of wine. In general, they produced higher concentration of fermentative volatile compounds than a commercial strain. The non-Saccharomyces yeasts in mixed fermentations with the S. cerevisiae strains also impacted wine composition and characteristics, leading lower ethanol content or enhancing aromatic balance and sensory equilibrium. The results highlight that grape harvest timing influences microbial diversity and fermentative performance and thus should be considered to better determine the optimum harvest date and ensure consistent wine characteristics. Full article

Background: Oxidative stress and inflammation contribute to osteoporosis. Berries provide polyphenols especially anthocyanins that may modulate bone remodeling. This review is the first to synthesize evidence specifically on berries and bone health, integrating human, animal, and in vitro data under the GRADE framework. Methods: We systematically searched PubMed, Embase, Web of Science, Scopus, and the Cochrane Library through 23 April 2025 for human, animal, and in vitro studies on berries or berry-derived compounds and bone outcomes. Risk of bias was assessed with RoB 2.0, ROBINS-I, SYRCLE, and an adapted ToxRTool; certainty of human evidence was appraised with GRADE. Results: Nineteen studies were included (5 human, 9 in vivo, 5 in vitro). Observational cohorts linked higher anthocyanin intake with greater BMD. Small randomized trials suggested modest benefits of blackcurrant and blueberry on whole-body BMD, bone turnover markers, and calcium retention, while results for biomarkers were mixed. Animal models generally showed attenuation of ovariectomy- or age-related bone loss, and in vitro experiments indicated inhibition of osteoclastogenesis with stimulation of osteoblast activity. By GRADE, certainty was low–moderate for BMD, low for biomarkers, and very low for fractures. Conclusions: Berry polyphenols may support skeletal health via antioxidant and anti-resorptive mechanisms, but current clinical evidence is limited by small samples, heterogeneity, and lack of fracture outcomes. Larger, longer, standardized RCTs with exposure profiling are needed before dietary recommendations can be made. Full article

The purpose of this paper is to evaluate the nutritional, functional, and technological potential of whey resulting as a by-product in the dairy industry, as such or mixed with berries (blueberries, strawberries, and raspberries) to obtain healthy jellies with added value. In this regard, the following parameters were analyzed: protein content, total amino acids, total mineral substances, macro- and microelements, antioxidant capacity, and total polyphenols. Also, the storage stability, textural and color parameters, FTIR spectra, and microstructures of jellies were analyzed. The results obtained showed that the protein content ranged from 4.18% to 4.51%, with a general increase observed in the variants with added whey and berries. Regarding total mineral substances, a significant increase was noted in jellies with added whey (0.34%) and strawberries (0.35%), compared to the control (0.15%). Whey jellies presented the highest levels of K, Ca, Mg, Zn, and Fe, while samples with added fruits completed the microelement (Mn, Cu, Ni, and Cr) content. The storage stability at 4 °C and the evolution of pH and acidity confirm that the products maintain their structure, while when stored at ambient temperature an acceleration of the decrease in pH and an increase in acidity are observed after 14 days. The jellies with combined additions (whey and berries) presented the most favorable microstructure, which supports the use of synergistic functional ingredients in the development of innovative products with high nutritional and sensory value. The FTIR spectra reflect the composition of the ingredients used. Based on obtained results, it can be concluded that whey represents a versatile and sustainable resource for obtaining functional jellies, offering both nutritional benefits and favorable economic and ecological perspectives. Full article

The manuscript explores the valorization of forest logging residues, collected during forest management operations between summer 2023 and spring 2025 in mixed deciduous and coniferous forests, as a raw material for producing valuable bioactive products. These products offer a sustainable alternative to synthetic pesticides and fertilizers. Seven batches of biomass, comprising understory trees and branches from deciduous (mainly aspen, birch, and grey alder) and coniferous (mainly Scots pine) species, were collected during different seasons, crushed, and extracted using an ethanol–water solution. The yield of hydrophilic extracts containing proanthocyanidins (PACs) ranged from 18 to 25% per dry biomass. The highest PACs concentration (42% of extract dry mass) was found in small branches with a high bark content. The extracts and PACs at concentrations of 6.25–12.50 mg mL⁻¹ showed fungicidal activity against several pathogenic fungi, including Botrytis cinerea Pers., Mycosphaerella sp. Johanson, Heterobasidion annosum (Fr.) Bref., and Heterobasidion parviporum Niemelä & Korhonen. Residual biomass after extraction, enriched with sea buckthorn berry pomace and a siliceous complex, was characterized and evaluated for its impact on the growth of Scots pine seedlings and selected agricultural crops. Results from forest and agricultural field trials in 2023–2025 confirmed a positive effect of the fertilizer on crop yield and quality at a low application rate (40 kg ha⁻¹ per crop). Fertilizer increased the yield of radish, dill, potatoes, and wheat by up to 44% (highest for potatoes and dill) compared to the reference, confirming its agronomic value. Full article

Fruit and sour beers are popular due to their unique sensory characteristics. Owing to changes in physicochemical parameters, mixed culture fermentation is a promising research area. The aim of the study was to evaluate how ethanol and lactic acid fermentation, combined with the addition of berry fruits during the beer production process, influence the physicochemical and sensory characteristics of sour fruit beers. Three worts differing in hopping system were produced: one classic sweet wort and two lacto-fermented. Strawberries or raspberries were added to the young beer. This research showed that acidification of wort, fruit addition, and limiting of hopping time had a positive effect on both technological and sensory characteristics. Despite pH differences, alcohol content in beers was similar (2.52–3.21% v/v). Production method influenced mainly lactic acid (0–2.30 g/L), pH (3.53–4.79), and glycerol (0.83–1.62 g/L) contents. Non-acidified beers had the highest dextrin (17.64–23.13 g/L) and glycerol (1.36–1.62 g/L) levels. The addition of strawberries increased phenolics (205.21–237.03 mg GAE/L), FRAP (0.82–1.17 mmol TE/L), and refreshment sensation, while raspberries mainly enhanced sensory atributes (colour, foam, fruitiness, aroma). Lactic fermentation did not show a clear effect on polyphenol content or antioxidant activity. The research offers practical insights into functional beer development, with its novelty of using mixed fermentation and fruit addition to shape characteristics. Full article

This study investigated the effects of six cap management protocols targeting contrasting oxidation-reduction potential (ORP) evolutions during alcoholic fermentation of Pinot noir wines. Treatments included twice-daily punch-downs (PD) and pump-overs (PO), 1 h air or N₂ injections (AirMix, N₂Mix), air injections triggered by ORP ≤ −40 mV (RedoxConAir), and equal N₂ injections concurrent to RedoxConAir wines (RedoxConN₂). AirMix wines maintained ORP values above 0 mV throughout fermentation, showed an oxidatively favored glutathione-to-glutathione disulfide ratio (GSH:GSSG) of 0.3:1, and had 21% lower total phenolics and 24% lower anthocyanins than PD wines. In contrast, N₂Mix wines maintained the lowest ORP, near −100 mV, and showed a reductively favored GSH:GSSG ratio (7:1). PD wines extracted 48% more flavan-3-ols than PO wines, consistent with greater berry integrity disruption and seed submersion. Volatile composition was also impacted: ethyl n-octanoate showed the highest OAV among esters, ranging from 147 in PO wines to 116 in AirMix wines. Results suggest the GSH:GSSG ratio served as an indicator of redox history, with potential implications for color and aroma preservation during aging. Inert gas mixings resulted in equal or greater total phenolic content, while excessive air injections may provide a tool to soften astringency. Full article

This study investigates the roles of grapevine berry inner necrosis virus (GINV) and grapevine yellow speckle viroid 1 (GYSVd1) in regulating the soluble sugar and organic acid metabolism of grape berries and wine. The contents of soluble sugar and organic acid components and the activity and expression levels of critical enzymes of the soluble sugar acid metabolism pathway were measured in ‘Welschriesling’ grape berries and wine carrying the virus GINV, the viroid GYSVd1, and a mixed infection of both GINV and GYSVd1 (GINV + GYSVd1), respectively. The results show that the virus GINV and the viroid GYSVd1 decreased the soluble sugar and increased the organic acid in berries and wine. GINV decreased glucose content and increased malic acid content by regulating AI, NADP-IDH, PEPC, and NAD-MDH activity, as well as VvHT4, VvSWEET10, VvPEPC, and VvMDH expression levels. GYSVd1 decreased glucose content and increased malic acid content by regulating AI and CS activity and VvHT4, VvSWEET15, and VvPEPC expression. The results suggest that the viroid GYSVd1 negatively impacts berries and wine more than the virus GINV. Moreover, in the mixed infection with GINV + GYSVd1, the negative effects of GINV and GYSVd1 on soluble sugars do not seem to be observed. Full article

Consumer demand for low-alcohol acidic beers is driving the use of non-conventional yeasts in the brewing process. In this study, the addition of mixed berries and fermentation with L. thermotolerans L31 are performed in crafting a low-alcohol acidic beer. Four different beers were brewed in the primary stage with either Saccharomyces cerevisiae or L. thermotolerans and with or without added berry mixture. Beer was fermented for 8 days at 20 °C, stored, and bottled. pH, density, alcoholic content, bitterness, and color of final beer were analyzed for all samples using analytical methods. Volatile compounds, anthocyanin content, and antioxidant activity were also evaluated. Sensory analysis was performed and correlated (PCA) with the analytical results. The obtained data indicated that beers brewed with L. thermotolerans were significantly more acidic and less bitter than S. cerevisiae beers. No difference in alcoholic content was found. Fruity aroma-associated compounds were present in L. thermotolerans beers, which correlated with the sensory analysis. Fruit beers were also redder and showed higher anthocyanin content and stronger antioxidant activity due to the presence of anthocyanins such as cyanidin, delphinidin, and malvidin from fruit, and other antioxidant compounds. Full article