Search Results (183)

Most of the publications on Anisakis simplex (Rudolphi, 1809) focus upon systematic aspects and zoonotic risk but there is lack of detailed information on infection and cellular immune response of the gonads. The current investigation detected whether gonadal infection by A. simplex affects reproductive development and how Atlantic mackerel, Scomber scombrus (L.), responds immunologically. Thirty-four specimens out of 62 S. scombrus (55%) individuals had the larvae on or in the gonads, and live and calcified dead larvae often co-occurred. Nematode larvae were found in both mature and immature gonads. The intensity of infection varied from 1 to 31 nematodes per gonad (5.68 ± 5.53, mean ± standard deviation). In ovaries that harboured the nematode (inside the organ and in the mesenteries around it), in close vicinity to the larvae, mast cells (MCs), rodlet cells (RCs), macrophages, and macrophage aggregates (MAs) occurred. Fewer of the above cells were observed in the mesenteries of parasitized testes. In the gonads of infected fish, within the granulomas that surrounded the calcified and live larvae, putative epithelioid cells, fibroblasts, MCs, MAs and collagen fibres were present. The absence of a correlation between the intensity of infection and the host gonadosomatic index in female and male gonads might indicate that A. simplex larvae do not affect the production of gametes and gonad development in Atlantic mackerel. Our results suggest that localized encapsulation represents an effective tolerance strategy allowing parasite persistence while preserving host reproductive function. Full article

Alepes djedaba is a coastal fish species of high economic value. However, information on its early developmental biology remains incomplete, which limits its application in large-scale seed production. In this study, fertilized eggs were obtained through hormone-induced spawning. Under controlled conditions, microscopic observation and histological sectioning were used to systematically record and analyze embryonic development, larval growth characteristics, and the gonadal development potential of the artificially bred first-generation (F1) offspring. The results showed that the fertilized eggs of A. djedaba are spherical and pelagic with a single oil globule. The egg diameter is (672.02 ± 10.03) μm, and the oil globule diameter is (189.76 ± 8.82) μm. At a water temperature of 24.50–24.97 °C, embryonic development is divided into 6 periods (20 stages), taking 19 h 28 min to complete hatching with an average hatching rate of 22.9% ± 15.6%. The total length of newly hatched larvae is (1.43 ± 0.09) mm, with an average daily growth of 0.39 mm from 0 to 21 days post-hatching (DPH). Furthermore, the testes of the F1 generation cultured in captivity exhibit complete tissue structures and mature spermatozoa, while the ovaries show orderly development primarily dominated by stage II–III oocytes with some early stage IV oocytes. This confirms at the histocytological level that the artificially bred offspring possess normal gonadal development, providing strong evidence of its reproductive potential. This study clarifies the key stages of early development and the adaptability of A. djedaba in captive environments, providing critical theoretical support and basic data for subsequent large-scale seed production and larval rearing optimization. Full article

The peripartum period in prolific ewes is associated with increased metabolic demands, and dietary lipid supplementation may increase the energy density of the prepartum diet. This study evaluated the effects of prepartum supplementation with alternative lipid matrices on the physicochemical characteristics and comprehensive fatty acid (FA) profiles of mature and transitional sheep milk. Thirty-two pregnant Olkuska ewes were randomly assigned to four cohorts (n = 8/group): Control (basal diet), flaxseed oil (low n-3/high n-6 variety; FO), black soldier fly larvae oil (BSFL oil), and Mix oil (2:1 ratio of FO to BSFL oil). Dietary treatments exerted no significant effects (p > 0.05) on transitional milk composition or lipid quality indices. In contrast, in mature milk, BSFL oil supplementation significantly increased milk fat content (6.09%) compared to the Control group (4.70%; p < 0.01). Furthermore, all oil treatments significantly reduced total saturated FAs (p < 0.01), while the BSFL oil and Mix oil supplementation significantly increased the monounsaturated FA fraction (p < 0.01). The differences, particularly the increase in cis-9, trans-11 CLA concentration and the significant reduction in both the atherogenic and thrombogenic indices (p < 0.01), were observed exclusively in mature milk. These findings suggest that the combination of sustainable insect lipids with high-linoleic plant oils may represent a viable strategy to transform sheep milk into a functional food with potential cardiovascular health benefits for consumers. These findings additionally indicate that prepartum lipid supplementation may exert a persistent carry-over effect into early lactation, influencing the fatty acid composition of mature sheep milk. Full article

Background: The visual system plays critical roles in fish feeding, predator avoidance, and reproduction, with the phototransduction pathway serving as the core molecular mechanism of visual signal transduction. This study represents the first systematic analysis of phototransduction-related genes in large yellow croaker (Larimichthys crocea). Methods: Bioinformatics, transcriptomic analysis, and RT-qPCR were used to identify phototransduction-related genes in large yellow croaker and analyze their developmental expression patterns and responses to different light spectra. Results: Thirty phototransduction-related genes were identified, including genes involved in light perception, signal amplification, and signal termination and recovery. Structural analyses indicated that these genes were highly conserved in teleosts. Most genes showed low expression during 1–7 days post-hatching (dph), increased expression during 7–24 dph, and stable expression during 24–35 dph. Collectively, these results identify 15–24 dph as a critical developmental window for visual system maturation in large yellow croaker larvae. Under different spectral treatments at 15 dph, phototransduction-related genes exhibited wavelength-dependent expression patterns. Under the present experimental conditions, relatively higher expression levels of most phototransduction-related genes were observed under blue- and green-light treatments, whereas relatively lower expression levels were observed under the red-light treatment. Key downstream genes, including gucy2f, guca1d, calml4a, and rgs9b, were significantly affected by different light spectra. Conclusions: This study revealed the developmental dynamics and spectral response characteristics of phototransduction-related genes in the large yellow croaker. These findings provide new insights into teleost visual development and light adaptation and provide a molecular basis for future studies aimed at optimizing light conditions during larval rearing in large yellow croaker aquaculture. Full article

Understanding the early life history of the endangered largemouth bronze gudgeon (C. guichenoti) is crucial for optimizing artificial rearing and conservation strategies. To provide a unified scientific basis for these efforts, this study characterized the ontogeny of C. guichenoti larvae from hatching to 30 days post-hatch (dph) by integrating analyses of allometric growth, digestive histology, and enzyme activities. Morphometric analysis revealed that total length increased exponentially with age. Positive allometric growth was observed in swimming organs (pectoral, dorsal, and caudal fins) and eye diameter, indicating a priority in developing locomotion and sensory capabilities for survival in lotic environments. Histological examination showed that the digestive tract became patent by 4 dph, and functional structures were established by 5 dph, coinciding with yolk sac absorption and the initiation of exogenous feeding. The digestive system reached near-adult maturity by 20 dph. Enzyme activity profiles showed that trypsin activity increased continuously, peaking at 30 dph, while amylase and alkaline phosphatase peaked at 20 dph. Lipase activity remained low until a slight increase at 30 dph. These results indicate that C. guichenoti larvae adopt a “locomotion-first” strategy, with the digestive system becoming functional at 5 dph and maturing by 20 dph. These findings collectively define the critical windows for artificial propagation, specifically guiding the timing of initial feeding and feed formulation. Full article

To address the “3R” issues (resistance, resurgence, and residue) associated with chemical control of the sea buckthorn fruit fly (R. batava obscuriosa), this study proposes a novel physical barrier technology aimed at reducing pesticide application intensity, mitigating environmental pollution, and enhancing fruit quality. Yellow sticky traps were deployed to monitor adult occurrence dynamics and delineate the critical control window, while black polyethylene (PE) ground cover was installed on the orchard floor around the base of sea buckthorn trunks to prevent adult emergence from the soil. Control efficacy was evaluated by comparing adult trap catches and fruit infestation rates between the black PE ground cover treatment and the untreated control. Monitoring results revealed that adult emergence commenced on 29 June, entered the peak period on 9 July, attained maximum trap catch on 24 July, and persisted into the late emergence phase through mid-to-late August. Control data demonstrated that mean trap catches in the black PE ground cover treatment were lower than those in the control. From 2024 to 2025, fruit infestation rates declined from 74.5% and 62.3% in the control plot to 19.0~22.0% and 16.2~19.3% in the treatment plots, respectively, with control efficacy consistently exceeding 65%. This study demonstrates that black PE ground cover reduces adult abundance and fruit infestation rates of R. batava obscuriosa, with control efficacy consistently exceeding 65%. The observed effects are consistent with a soil-surface barrier effect and likely attributed to dual physical mechanisms: it may reduce adult emergence from the soil into the canopy and may obstruct mature larvae from entering the soil to pupate. This technology represents an environmentally sound, sustainable green control option suitable for integration into IPM programs for the sea buckthorn industry. Full article

The chemical control of Leguminivora glycinivorella (Mats.) (Lepidoptera: Tortricidae) has been a major threat to the soybean industry in China over the years. Therefore, we need to develop green, safe, and environmentally friendly alternatives for pest control. Amine hormones, such as melatonin, represent an adjunct strategy for the green control of L. glycinivorella. We aimed to investigate the effects of exogenous melatonin on the development, survival, reproduction, nutrient accumulation, and cold tolerance of L. glycinivorella and to provide insights into the role of melatonin in insect adaptive regulation. Newly hatched L. glycinivorella larvae were fed on soybean pods immersed in solutions with different concentrations of exogenous melatonin. The developmental duration of larvae and pupae, survival rate, pupation rate, adult eclosion rate, body weight and length of larvae and pupae, nutrient composition of mature larvae, adult reproductive parameters, and supercooling and freezing points were measured. With increasing melatonin concentrations, the developmental durations of larvae and pupae were significantly prolonged, while the larval survival rate, pupation rate, and adult eclosion rate significantly decreased. The body weight and length of both larvae and pupae declined with increasing melatonin concentrations, reaching the lowest values in the 200 mg/L group. Melatonin treatment significantly reduced protein and lipid contents in mature larvae but significantly increased glycogen content. Reproductive parameters, including pre-oviposition period, oviposition period, fecundity, and female adult longevity, all decreased significantly with increasing melatonin concentrations. Additionally, melatonin treatment significantly lowered the supercooling point and freezing point of mature larvae. Exogenous melatonin significantly inhibits the growth, development, survival, reproduction, and nutrient metabolism of L. glycinivorella, yet enhances its low-temperature tolerance, suggesting that melatonin may serve as a potential tool for population management of L. glycinivorella through its dual regulatory role in insect physiology. Full article

The immature stages and biology of Neoplinthus tigratus porculus (Fabricius, 1801) (Coleoptera: Curculionidae: Molytinae) associated with common hop (Humulus lupulus L.) are described for the first time. Biological observations show that the species develops mainly within the root collar and roots of Humulus lupulus, where larvae feed internally and older instars overwinter. Infested plants are characterized by swollen and weakened roots, often containing multiple larvae. The species should be considered a potential pest of common hop, an economically important crop; however, the current observations indicate that its populations are generally very low, consistent with the status of several related Molytinae and Cleonini taxa, which are predominantly regarded as rare or locally occurring under contemporary agricultural conditions. Nevertheless, changes in agroecosystem management may significantly alter its abundance, as documented in other weevil taxa, where reductions in plant protection measures have led to local pest outbreak. The morphology and diagnostic characters of mature larvae and pupae are documented and compared with related Molytinae and selected Cleonini (Lixinae). The mature larva generally fits the diagnostic characters of Molytinae larvae but differs in several traits, particularly the very short endocranial line and the relative length of frontal setae (fs_1–5), with fs₄ distinctly shorter than fs₅. Full article

Silver pomfret (Pampus argenteus, family Stromateidae) is a highly valuable marine fish species with significant commercial demand; however, its aquaculture remains undeveloped due to limited knowledge of captive breeding and seed production. To our knowledge, this is the first successful report on the induction of maturation, natural spawning, and larval rearing of silver pomfret under captive conditions in Korea. Wild broodstock (33 individuals in 2020; 250 individuals in 2021) were collected from the southern coastal waters of Korea using set nets. In the first year, water temperature management alone successfully induced gonadal maturation, as evidenced by a significant increase in the gonadosomatic index (GSI) and the presence of vitellogenic oocytes (400–500 μm) in April. In the second year, natural spawning was observed on fifteen occasions from May to September 2022, yielding a total number of 157,050 eggs. Fertilized eggs were spherical, transparent, and pelagic, with diameters ranging from 1.29 to 1.37 mm. Hatched larvae (total length: 4.85 ± 0.22 mm) exhibited poor feeding responses to rotifers and high early mortality within two weeks post-hatching, with the maximum rearing period reaching 24 days post-hatching. These findings demonstrate that water temperature management alone is sufficient to induce maturation and natural spawning of silver pomfret, and highlight the critical need for optimizing larval feeds, improving broodstock nutritional management, and conducting endocrine profiling during reproduction to establish a complete aquaculture protocol for this species. Full article

Remote sensing advancements have enhanced defoliation monitoring in forests, but distinguishing insect-specific damage from general canopy stress remains challenging due to overlapping spectral signatures. This study addresses this gap by analyzing multispectral reflectance changes in Eucalyptus dunnii caused by Gonipterus sp. n. 2 larval feeding and artificial defoliation (AD). A randomized complete block design with five replicates tested four treatments: No Damage, Medium (100 larvae/tree) and High (200 larvae/tree) larval inoculation, and AD (80% leaf removal). Twenty potted E. dunnii trees were monitored over 16 days using UAV-based multispectral 10-band imagery. Five multispectral flights were conducted during the experiment. The reduction in visible and near-infrared (NIR) reflectance likely reflects structural changes in canopy composition, namely an increased proportion of mature foliage. Both larval feeding and AD treatments decreased reflectance in these spectral regions, probably due to the removal of young leaves and exposure of older, darker leaves. This explanation is inferred from morphological observations; further biochemical measurements would be required to confirm the underlying mechanisms. Larval feeding and AD reduced chlorophyll-related vegetation indices (CVI, NDRE), decreased anthocyanin-related vegetation indices (mARI, ARI), and also caused a drop in relative carotene content (MTVI, CTRI/RE). The effects were strongest in the AD and peaked soon after the treatment, indicating that these pigment effects can mostly and also be attributed to the older leaves becoming more exposed. Statistically significant interactions between date and treatment were found for the pigment-sensitive indices, the Anthocyanin Reflectance Index (ARI) and the Chlorophyll Vegetation Index (CVI). They displayed opposite reflectance trends—CVI increased while ARI decreased—but followed a consistent pattern aligned with insect feeding. EVI values also exhibited a distinguishable pattern that matched this trend. Due to the inherent difficulty of studying insect feeding in natural settings, AD trials may serve as a practical proxy for assessing the impact of pest-induced damage on vegetation reflectance and physiological indices. Full article

Caffeine (CAF) is one of the most widely consumed psychoactive substances worldwide. It is primarily metabolized to paraxanthine (PAR), theobromine (THR), and theophylline (THY). While CAF metabolism has been extensively characterized in humans and rodent models, corresponding data in zebrafish (Danio rerio) larvae remain limited. This study provides a comprehensive characterization of CAF biotransformation in zebrafish larvae using validated LC-QQQ-MS for quantitative metabolite profiling and FTIR imaging for spatially resolved tissue distribution mapping. Zebrafish larvae at 4 days post fertilization (dpf) were exposed to CAF at concentrations of 15, 25, and 50 mg/L for 18 h. The LC–MS/MS analysis demonstrated dose-dependent conversion of CAF to PAR (up to 4.54%), THR (up to 1.32%), and THY (up to 0.27%). The PAR/CAF and THR/CAF ratios increased as CAF concentration rose, while the THY/CAF ratio declined. In parallel, FTIR imaging was applied to visualize the spatial distribution of CAF and selected metabolites in larval tissue sections, confirming the presence of CAF, PAR, and THR and revealing their localization within the tissue. However, THY was not detected by this method. Metabolite localization differed across larval tissues, indicating compartmentalized metabolic processes. This study provides the first comprehensive characterization of CAF metabolism in zebrafish larvae, revealing low but detectable CYP450-mediated metabolic activity prior to full hepatic maturation. The findings support the utility of zebrafish larvae as a model for developmental pharmacokinetic studies. Full article

The yellow peach moth, Conogethes punctiferalis, is a destructive polyphagous pest and poses a severe threat to the fruit industry and field crops worldwide with its continuously increasing population and expanding host range in recent years. Despite the severe damage caused by C. punctiferalis larvae, their antennae and mouthparts, equipped with abundant sensilla responsible for feeding behavior, have not been investigated in detail. In our study, the antennae, mouthparts, and associated sensilla of first-instar and mature larvae of C. punctiferalis were examined with light and scanning electron microscopy. Our results revealed no obvious morphological differences between the two instars in the basic composition of the antennae and mouthparts, or in the types, distribution, and numbers of sensilla. The antenna is three-segmented, with no sensilla on the scape, three sensilla basiconica and two sensilla chaetica on the pedicel, and three sensilla basiconica and one sensillum styloconicum on the flagellum. The mouthparts of C. punctiferalis are typically mandibulate and consist of a labrum-epipharynx, paired mandibles, a pair of maxillae, a labium, and a hypopharynx. Six types of sensilla were primarily concentrated on the labrum-epipharynx, maxilla, and labial palp, including sensilla chaetica, sensilla basiconica, sensilla styloconica, sensilla digitiformia, sensilla epipharyngea, and sensilla placodea. We conducted a systematic analysis of the characteristics of sensilla and discussed their variation in the context of Lepidoptera phylogeny. The potential functions of the sensilla have also been inferred. The study could advance our understanding of the behavioral ecology of C. punctiferalis and provide potentially useful information on the development of pest control technologies. Full article

Cuticular proteins (CPs)—key components of the insect exoskeleton—not only regulate development but also serve as structural barriers that enhance resistance against environmental stressors. This study identified CP gene families in Apis mellifera and analyzed their expression patterns during the worker capped brood development stages from mature larva to pre-eclosion. Using a comprehensive genome-wide bioinformatic approach, we identified 85 CP genes in A. mellifera which comprise six families: CPR (n = 43), CPAPs (n = 27), CPF (n = 2), Tweedle (n = 2), CPLCP (n = 8) and Apidermin (n = 3). Analysis of CP gene evolutionary relationship revealed that each CP family forms a distinct, relatively independent clade. Domain and motif analyses confirmed that all CPR members harbor a conserved Chitin_Bind_4 domain, consistent with CPR family structures in other taxa. Additionally, CPAP members possess one or three Chitin-binding Peritrophin-A domain (CBM_14), CPF members possess a conserved Pupal cuticle protein C1 domain (Cuticle_3), and Tweedle members contain a conserved domain of unknown function (DUF243). In addition, the analysis found no conserved domain within the CPLCP and Apidermin families. RNA-seq data revealed dynamic expression patterns of AmCPs during pupal development, with each gene family displaying a relatively characteristic temporal profile. Quantitative PCR validation of eight highly expressed CPR genes at 9 days post-capping confirmed the RNA-seq results. This work provides a comprehensive bioinformatic characterization and transcriptional analysis of CP genes in A. mellifera, offering a foundation for future functional studies on cuticle formation and identifying candidate genes potentially involved in cuticle development in honeybees. This work relies on transcriptomic data and in silico analyses. All proposed biological roles are hypothetical and require experimental validation. Full article

Last instar larva and pupa of Hypera libanotidis Reitter, 1896 (Curculionidae: Hyperini) are described for the first time and compared with 44 other hyperine taxa. Larval morphology generally matches the diagnostic characters of Hyperini but shows distinctive traits, including thorn-like setae on conspicuous black protuberances, relatively long body setae, and dense spiculate coverings in larvae and pupae. Several unusual pupal characters, such as an atypical mesocoxal seta and a peculiar spiracular covering, further distinguish this species. These characters indicate close morphological affinity with species within the subgenus Eririnomorphus and also the genus Metadonus, suggesting a potentially close phylogenetic relationship. Biological observations confirm typical hyperine traits, including ectophytic larval feeding, cryptic coloration, and cocoon construction prior to pupation. Larval coloration, especially in early instars, partly corresponds with the host plant Libanotis pyrenaica. Larvae pupate in mesh-like cocoons on host plant remains, and no larval parasitism was observed. The species is associated with xerothermic loess grasslands, with adults apparently overwintering. Full article

Background: Di(2-ethylhexyl) phthalate (DEHP) is a high-production-volume plasticizer and ubiquitous environ-mental contaminant with established endocrine-disrupting potential. While zebrafish transcriptomic studies have typically used high concentrations and long exposure windows, less is known about genome-wide responses during late embryogenesis/early larval maturation under environmentally relevant exposures. Here we profiled whole-organism transcriptomic responses to a short DEHP exposure during a developmentally sensitive transition (96–120) hours post-fertilization, hpf) and interpreted responses using differential expression, enrichment analyses, and endocrine-focused protein–protein interaction (PPI) network modeling. Methods: Wild-type AB zebrafish lar-vae (96 hpf) were exposed to DEHP at [10⁻⁹ M] or solvent control for 24 h. Larvae were pooled per replicate (25 lar-vae/pool) and processed for poly(A)-selected RNA-seq. Reads were quality-controlled, aligned to the Danio rerio reference genome, and quantified at gene- level. Differential expression was performed using DESeq2. Functional enrichment used KEGG over-representation analysis (ORA) and gene set enrichment analysis (GSEA). Zebrafish genes were mapped to human orthologs for GO/KEGG and STRING-based endocrine subnetworks, which were visualized and interrogated using STRINGdb and visNetwork. Results: Low-dose, short-term exposure does not produce large gene-level effects but induces coordinated, pathway-level transcriptional remodeling. KEGG ORA showed significant enrichment of MAPK signaling and regulation of actin cytoskeleton with additional enrichment of axon guidance and neuroactive ligand–receptor interaction. GSEA detected coordinated downregulation of KEGG neurodegeneration collections with negative normalized enrichment scores reflecting shared gene sets re-lated to mitochondrial function, proteostasis, cytoskeletal organization, and stress-response pathways. Endo-crine-focused STRING subnetworks indicated consistent downregulation of CYP19A1 within estrogen metabo-lism/biosynthesis modules and downregulation of upstream androgen biosynthetic enzymes HSD3B2 and CYP17A1, alongside upregulation of HSD17B3 and proteostasis-associated factors including DNAJA1. Endocrine network to-pology highlighted regulatory and cofactor nodes affecting receptor-linked transcription, consistent with indirect endocrine modulation rather than large receptor-transcript changes. Conclusions: In summary, this study demon-strates that exposure to low-dose DEHP during a critical period of zebrafish embryonic development is associated with modest but coordinated transcriptomic changes across multiple biological pathways. Pathway enrichment and network-based analyses highlight estrogen- and androgen-associated processes, along with broader signaling, met-abolic, and structural pathways, as transcriptionally responsive during this window. Importantly, these findings reflect molecular-level associations rather than direct evidence of functional or physiological endocrine disruption. Instead, they identify candidate pathways and regulatory networks that may be sensitive to low-level environmen-tal exposure and warrant further investigation. Collectively, this work underscores the value of systems-level tran-scriptomic approaches for detecting subtle, pathway-wide responses to environmentally relevant exposures during development. Full article