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Search Results (286)

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22 pages, 51773 KB  
Article
On a Software Framework for Automated Pore Identification and Quantification for SEM Images of Metals
by Michael Mulligan, Oliver Fowler, Joshua Voell, Mark Atwater and Howie Fang
Computers 2025, 14(10), 442; https://doi.org/10.3390/computers14100442 - 16 Oct 2025
Viewed by 92
Abstract
The functional performance of porous metals and alloys is dictated by pore features such as size, connectivity, and morphology. While methods like mercury porosimetry or gas pycnometry provide cumulative information, direct observation via scanning electron microscopy (SEM) offers detailed insights unavailable through other [...] Read more.
The functional performance of porous metals and alloys is dictated by pore features such as size, connectivity, and morphology. While methods like mercury porosimetry or gas pycnometry provide cumulative information, direct observation via scanning electron microscopy (SEM) offers detailed insights unavailable through other means, especially for microscale or nanoscale pores. Each scanned image can contain hundreds or thousands of pores, making efficient identification, classification, and quantification challenging due to the processing time required for pixel-level edge recognition. Traditionally, pore outlines on scanned images were hand-traced and analyzed using image-processing software, a process that is time-consuming and often inconsistent for capturing both large and small pores while accurately removing noise. In this work, a software framework was developed that leverages modern computing tools and methodologies for automated image processing for pore identification, classification, and quantification. Vectorization was implemented as the final step to utilize the direction and magnitude of unconnected endpoints to reconstruct incomplete or broken edges. Combined with other existing pore analysis methods, this automated approach reduces manual effort dramatically, reducing analysis time from multiple hours per image to only minutes, while maintaining acceptable accuracy in quantified pore metrics. Full article
(This article belongs to the Section Human–Computer Interactions)
17 pages, 5623 KB  
Article
Deep Learning-Based Back-Projection Parameter Estimation for Quantitative Defect Assessment in Single-Framed Endoscopic Imaging of Water Pipelines
by Gaon Kwon and Young Hwan Choi
Mathematics 2025, 13(20), 3291; https://doi.org/10.3390/math13203291 - 15 Oct 2025
Viewed by 135
Abstract
Aging water pipelines are increasingly prone to structural failure, leakage, and ground subsidence, creating critical risks to urban infrastructure. Closed-circuit television endoscopy is widely used for internal assessment, but it depends on manual interpretation and lacks reliable quantitative defect information. Traditional vanishing point [...] Read more.
Aging water pipelines are increasingly prone to structural failure, leakage, and ground subsidence, creating critical risks to urban infrastructure. Closed-circuit television endoscopy is widely used for internal assessment, but it depends on manual interpretation and lacks reliable quantitative defect information. Traditional vanishing point detection techniques, such as the Hough Transform, often fail under practical conditions due to irregular lighting, debris, and deformed pipe surfaces, especially when pipes are water-filled. To overcome these challenges, this study introduces a deep learning-based method that estimates inverse projection parameters from monocular endoscopic images. The proposed approach reconstructs a spatially accurate two-dimensional projection of the pipe interior from a single frame, enabling defect quantification for cracks, scaling, and delamination. This eliminates the need for stereo cameras or additional sensors, providing a robust and cost-effective solution compatible with existing inspection systems. By integrating convolutional neural networks with geometric projection estimation, the framework advances computational intelligence applications in pipeline condition monitoring. Experimental validation demonstrates high accuracy in pose estimation and defect size recovery, confirming the potential of the system for automated, non-disruptive pipeline health evaluation. Full article
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17 pages, 3073 KB  
Article
An Open-Source Computer-Vision-Based Method for Spherical Microplastic Settling Velocity Calculation
by Catherine L. Stacy, Md Abdul Baset Sarker, Abul B. M. Baki and Masudul H. Imtiaz
Microplastics 2025, 4(4), 75; https://doi.org/10.3390/microplastics4040075 - 14 Oct 2025
Viewed by 162
Abstract
Microplastics (particles ≤ 5 mm) are ubiquitous and persistent, posing threats to ecosystems and human health. Thus, the development of technologies for evaluating their dynamics is crucial. Settling velocity is a critical parameter for predicting the fate of microplastics in aquatic environments. Current [...] Read more.
Microplastics (particles ≤ 5 mm) are ubiquitous and persistent, posing threats to ecosystems and human health. Thus, the development of technologies for evaluating their dynamics is crucial. Settling velocity is a critical parameter for predicting the fate of microplastics in aquatic environments. Current methods for computing this metric are highly subjective and lack a standard. The goal of this research is to develop an objective, automated technique employing the technological advances in computer vision. In the laboratory, a camera recorded the trajectories of microplastics as they sank through a water column. The settling velocity of each microplastic was calculated using a YOLOv12n-based object detection model. The system was tested with three classes of spherical microplastics and three types of water. Ground truth settling times, recorded manually with a stopwatch, allowed for quantification of the system’s accuracy. When comparing the velocities calculated using the computer vision system to the stopwatch ground truth, the average error across all water types was 5.97% for the 3 mm microplastics, 7.14% for the 4 mm microplastics, and 6.15% for the 5 mm microplastics. This new method will enable the research community to predict microplastic distribution and transport patterns, as well as implement more timely strategies for mitigating pollution. Full article
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21 pages, 10338 KB  
Article
Sustainable Mining of Open-Pit Coal Mines: A Study on Intelligent Strip Division Technology Based on Multi-Source Data Fusion
by Shuaikang Lv, Ruixin Zhang, Yabin Tao, Zijie Meng, Sibo Wang and Zhigao Liu
Sustainability 2025, 17(20), 9049; https://doi.org/10.3390/su17209049 - 13 Oct 2025
Viewed by 189
Abstract
The rational delineation of open-pit mining areas constitutes the core foundation for achieving safe, efficient, economical, and sustainable mining operations. The quality of this decision-making directly impacts the economic benefits experienced throughout the mine’s entire lifecycle and the efficiency of resource recovery. Traditional [...] Read more.
The rational delineation of open-pit mining areas constitutes the core foundation for achieving safe, efficient, economical, and sustainable mining operations. The quality of this decision-making directly impacts the economic benefits experienced throughout the mine’s entire lifecycle and the efficiency of resource recovery. Traditional open-pit mining area delineation relies on an experience-driven manual process that is inefficient and incapable of real-time dynamic data optimization. Thus, there is an urgent need to establish an intelligent decision-making model integrating multi-source data and multi-objective optimization. To this end, this study proposes an intelligent mining area division algorithm. First, a geological complexity quantification model is constructed, incorporating innovative adaptive discretisation resolution technology to achieve precise quantification of coal seam distribution. Second, based on the quantified stripping-to-mining ratio within grids, a block-growing algorithm generates block grids, ensuring uniformity of the stripping-to-mining ratio within each block. Subsequently, a matrix of primary directional variations in the stripping-to-mining ratio is constructed to determine the principal orientation for merging blocks into mining areas. Finally, intelligent open-pit mining area delineation is achieved by comprehensively considering factors such as the principal direction of mining areas, geological conditions, boundary shapes, and economic scale. Practical validation was conducted using the Shitoumei No. 1 Open-Pit Coal Mine in Xinjiang as a case study in engineering. Engineering practice demonstrates that adopting this methodology transforms mining area delineation from an experience-driven to a data-driven approach, significantly enhancing delineation efficiency. Manual simulation of a single scheme previously required approximately 15 days. Applying the methodology proposed herein reduces this to just 0.5 days per scheme, representing a 96% increase in efficiency. Design costs were reduced by approximately CNY 190,000 per iteration. Crucially, the intelligently recommended scheme matched the original design, validating the algorithm’s reliability. This research provides crucial support for theoretical and technological innovation in intelligent open-pit coal mining design, offering technical underpinnings for the sustainable development of open-pit coal mines. Full article
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12 pages, 1926 KB  
Article
Tracking False Lumen Remodeling with AI: A Variational Autoencoder Approach After Frozen Elephant Trunk Surgery
by Anja Osswald, Sharaf-Eldin Shehada, Matthias Thielmann, Alan B. Lumsden, Payam Akhyari and Christof Karmonik
J. Pers. Med. 2025, 15(10), 486; https://doi.org/10.3390/jpm15100486 - 11 Oct 2025
Viewed by 214
Abstract
Objective: False lumen (FL) thrombosis plays a key role in aortic remodeling after Frozen Elephant Trunk (FET) surgery, yet current imaging assessments are limited to categorical classifications. This study aimed to evaluate an unsupervised artificial intelligence (AI) algorithm based on a variational autoencoder [...] Read more.
Objective: False lumen (FL) thrombosis plays a key role in aortic remodeling after Frozen Elephant Trunk (FET) surgery, yet current imaging assessments are limited to categorical classifications. This study aimed to evaluate an unsupervised artificial intelligence (AI) algorithm based on a variational autoencoder (VAE) for automated, continuous quantification of FL thrombosis using serial computed tomography angiography (CTA). Methods: In this retrospective study, a VAE model was applied to axial CTA slices from 30 patients with aortic dissection who underwent FET surgery. The model encoded each image into a structured latent space, from which a continuous “thrombus score” was developed and derived to quantify the extent of FL thrombosis. Thrombus scores were compared between postoperative and follow-up scans to assess individual remodeling trajectories. Results: The VAE successfully encoded anatomical features of the false lumen into a structured latent space, enabling unsupervised classification of thrombus states. A continuous thrombus score was derived from this space, allowing slice-by-slice quantification of thrombus burden across the aorta. The algorithm demonstrated robust reconstruction accuracy and consistent separation of fully patent, partially thrombosed, and completely thrombosed lumen states without the need for manual annotation. Across the cohort, 50% of patients demonstrated an increase in thrombus score over time, 40% a decrease, and 10% remained unchanged. Despite these individual differences, no statistically significant change in overall thrombus burden was observed at the group level (p = 0.82), emphasizing the importance of individualized longitudinal assessment. Conclusions: The VAE-based method enables reproducible, annotation-free quantification of FL thrombosis and captures patient-specific remodeling patterns. This approach may enhance post-FET surveillance and supports the integration of AI-driven tools into personalized aortic imaging workflows. Full article
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27 pages, 957 KB  
Review
Deep Learning for Brain MRI Tissue and Structure Segmentation: A Comprehensive Review
by Nedim Šišić and Peter Rogelj
Algorithms 2025, 18(10), 636; https://doi.org/10.3390/a18100636 - 9 Oct 2025
Viewed by 442
Abstract
Brain MRI segmentation plays a crucial role in neuroimaging studies and clinical trials by enabling the precise localization and quantification of brain tissues and structures. The advent of deep learning has transformed the field, offering accurate and fast tools for MRI segmentation. Nevertheless, [...] Read more.
Brain MRI segmentation plays a crucial role in neuroimaging studies and clinical trials by enabling the precise localization and quantification of brain tissues and structures. The advent of deep learning has transformed the field, offering accurate and fast tools for MRI segmentation. Nevertheless, several challenges limit the widespread applicability of these methods in practice. In this systematic review, we provide a comprehensive analysis of developments in deep learning-based segmentation of brain MRI in adults, segmenting the brain into tissues, structures, and regions of interest. We explore the key model factors influencing segmentation performance, including architectural design, choice of input size and model dimensionality, and generalization strategies. Furthermore, we address validation practices, which are particularly important given the scarcity of manual annotations, and identify the limitations of current methodologies. We present an extensive compilation of existing segmentation works and highlight the emerging trends and key results. Finally, we discuss the challenges and potential future directions in the field. Full article
(This article belongs to the Special Issue Machine Learning in Medical Signal and Image Processing (4th Edition))
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22 pages, 2444 KB  
Article
UQ4CFD: An Uncertainty Quantification Platform for CFD Simulation
by Wei Xiao, Jiao Zhao, Luogeng Lv, Jiangtao Chen, Peihong Zhang and Xiaojun Wu
Aerospace 2025, 12(10), 886; https://doi.org/10.3390/aerospace12100886 - 30 Sep 2025
Viewed by 258
Abstract
The credibility of Computational Fluid Dynamics (CFD) has been a topic of debate due to the significant uncertainties inherent in its modeling processes and numerical implementations. Uncertainty Quantification (UQ) offers a scientific framework for quantitatively assessing and mitigating uncertainties in CFD simulations. However, [...] Read more.
The credibility of Computational Fluid Dynamics (CFD) has been a topic of debate due to the significant uncertainties inherent in its modeling processes and numerical implementations. Uncertainty Quantification (UQ) offers a scientific framework for quantitatively assessing and mitigating uncertainties in CFD simulations. However, this procedure typically requires numerous CFD simulations and considerable manual effort for both simulation management and data analysis. To overcome these challenges, this work develops a platform called UQ4CFD, a browser–server software that provides automated and customized uncertainty quantification capabilities for CFD studies. The UQ4CFD platform integrates different kinds of methodologies to perform comprehensive uncertainty analysis, including uncertainty propagation, sensitivity analysis, surrogate modeling, numerical discretization uncertainty analysis, model validation, model calibration, etc. A tightly coupled CFD-UQ workflow is built to automate the complete analytical process, encompassing parameter sampling, simulation execution, and results analysis, which significantly improves computational efficiency while reducing risks associated with data processing errors. Comprehensive validation employing both analytical benchmark functions and practical CFD cases has been conducted to demonstrate the platform’s effectiveness and adaptability in diverse UQ scenarios. Full article
(This article belongs to the Section Aeronautics)
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18 pages, 6231 KB  
Article
Optical Coherence Imaging Hybridized Deep Learning Framework for Automated Plant Bud Classification in Emasculation Processes: A Pilot Study
by Dasun Tharaka, Abisheka Withanage, Nipun Shantha Kahatapitiya, Ruvini Abhayapala, Udaya Wijenayake, Akila Wijethunge, Naresh Kumar Ravichandran, Bhagya Nathali Silva, Mansik Jeon, Jeehyun Kim, Udayagee Kumarasinghe and Ruchire Eranga Wijesinghe
Photonics 2025, 12(10), 966; https://doi.org/10.3390/photonics12100966 - 29 Sep 2025
Viewed by 280
Abstract
A vision-based autonomous system for emasculating okra enhances agriculture by enabling precise flower bud identification, overcoming the labor-intensive, error-prone challenges of traditional manual methods with improved accuracy and efficiency. This study presents a framework for an adaptive, automated bud identification method to assist [...] Read more.
A vision-based autonomous system for emasculating okra enhances agriculture by enabling precise flower bud identification, overcoming the labor-intensive, error-prone challenges of traditional manual methods with improved accuracy and efficiency. This study presents a framework for an adaptive, automated bud identification method to assist the emasculation process, hybridized optical coherence tomography (OCT). Three YOLOv8 variants were evaluated for accuracy, detection speed, and frame rate to identify the most efficient model. To strengthen the findings, YOLO was hybridized with OCT, enabling non-invasive sub-surface verification and precise quantification of the emasculated depth of both sepal and petal layers of the flower bud. To establish a solid benchmark, gold standard color histograms and a digital imaging-based method under optimal lighting conditions with confidence scoring were also employed. The results demonstrated that the proposed method significantly outperformed these conventional frameworks, providing superior accuracy and layer differentiation during emasculation. Hence, the developed YOLOv8 hybridized OCT method for flower bud identification and emasculation offers a powerful tool to significantly improve both the precision and efficiency of crop breeding practices. This framework sets the stage for implementing scalable, artificial intelligence (AI)-driven strategies that can modernize and optimize traditional crop breeding workflows. Full article
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14 pages, 4724 KB  
Article
Uncertainty-Guided Active Learning for Access Route Segmentation and Planning in Transcatheter Aortic Valve Implantation
by Mahdi Islam, Musarrat Tabassum, Agnes Mayr, Christian Kremser, Markus Haltmeier and Enrique Almar-Munoz
J. Imaging 2025, 11(9), 318; https://doi.org/10.3390/jimaging11090318 - 17 Sep 2025
Viewed by 562
Abstract
Transcatheter aortic valve implantation (TAVI) is a minimally invasive procedure for treating severe aortic stenosis, where optimal vascular access route selection is critical to reduce complications. It requires careful selection of the iliac artery with the most favourable anatomy, specifically, one with the [...] Read more.
Transcatheter aortic valve implantation (TAVI) is a minimally invasive procedure for treating severe aortic stenosis, where optimal vascular access route selection is critical to reduce complications. It requires careful selection of the iliac artery with the most favourable anatomy, specifically, one with the largest diameters and no segments narrower than 5 mm. This process is time-consuming when carried out manually. We present an active learning-based segmentation framework for contrast-enhanced Cardiac Magnetic Resonance (CMR) data, guided by probabilistic uncertainty and pseudo-labelling, enabling efficient segmentation with minimal manual annotation. The segmentations are then fed into an automated pipeline for diameter quantification, achieving a Dice score of 0.912 and a mean absolute percentage error (MAPE) of 4.92%. An ablation study using pre- and post-contrast CMR showed superior performance with post-contrast data only. Overall, the pipeline provides accurate segmentation and detailed diameter profiles of the aorto-iliac route, helping the assessment of the access route. Full article
(This article belongs to the Special Issue Emerging Technologies for Less Invasive Diagnostic Imaging)
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11 pages, 839 KB  
Article
Quantification of Thoracic Volume and Spinal Length of Pediatric Scoliosis Patients on Chest MRI Using a 3D U-Net Segmentation
by Romy E. Buijs, Dingina M. Cornelissen, Dimo Devetzis, Peter P. G. Lafranca, Daniel Le, Jiaxin Zhang, Mitko Veta, Koen L. Vincken and Tom P. C. Schlösser
Healthcare 2025, 13(18), 2327; https://doi.org/10.3390/healthcare13182327 - 17 Sep 2025
Viewed by 567
Abstract
Background/Objectives: Adolescent idiopathic scoliosis (AIS) can lead to significant chest deformations. The quantification of chest deformity and spinal length could provide additional insights for monitoring during follow-up and treatment. This study proposes a 3D U-Net convolutional neural network (CNN) for automatic thoracic and [...] Read more.
Background/Objectives: Adolescent idiopathic scoliosis (AIS) can lead to significant chest deformations. The quantification of chest deformity and spinal length could provide additional insights for monitoring during follow-up and treatment. This study proposes a 3D U-Net convolutional neural network (CNN) for automatic thoracic and spinal segmentations of chest MRI scans. Methods: In this proof-of-concept study, axial chest MRI scans from 19 girls aged 8–10 years at risk for AIS development and 19 asymptomatic young adults were acquired (n = 38). The thoracic volume and spine were manually segmented as the ground truth (GT). A 3D U-Net CNN was trained on 31 MRI scans. The seven remaining MRI scans were used for validation, reported by the Dice similarity coefficient (DSC), the Hausdorff distance (HD), precision, and recall. From these segmentations, the thoracic volume and 3D spinal length were calculated. Results: Automatic chest segmentation was possible for all chest MRIs. For the chest volume segmentations, the average DSC was 0.91, HD was 51.89, precision was 0.90, and recall 0.99. For the spinal segmentation, the average DSC was 0.85, HD was 25.98, precision was 0.74, and recall 0.99. Chest volumes and 3D spinal lengths differed by on average 11% and 12% between automatic and GT, respectively. Qualitative analysis showed agreement between the automatic and manual segmentations in most cases. Conclusions: The proposed 3D U-Net CNN shows a high accuracy and good predictions in terms of HD, DSC, precision, and recall. This suggested 3D U-Net CNN could potentially be used to monitor the progression of chest deformation in scoliosis patients in a radiation-free manner. Improvement can be made by training the 3D U-net with more data and improving the GT data. Full article
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15 pages, 473 KB  
Article
Every Step Counts—How Can We Accurately Count Steps with Wearable Sensors During Activities of Daily Living in Individuals with Neurological Conditions?
by Florence Crozat, Johannes Pohl, Chris Easthope Awai, Christoph Michael Bauer and Roman Peter Kuster
Sensors 2025, 25(18), 5657; https://doi.org/10.3390/s25185657 - 11 Sep 2025
Viewed by 745
Abstract
Wearable sensors provide objective, continuous, and non-invasive quantification of physical activity, with step count serving as one of the most intuitive measures. However, significant gait alterations in individuals with neurological conditions limit the accuracy of step-counting algorithms trained on able-bodied individuals. Therefore, this [...] Read more.
Wearable sensors provide objective, continuous, and non-invasive quantification of physical activity, with step count serving as one of the most intuitive measures. However, significant gait alterations in individuals with neurological conditions limit the accuracy of step-counting algorithms trained on able-bodied individuals. Therefore, this study investigates the accuracy of step counting during activities of daily living (ADL) in a neurological population. Seven individuals with neurological conditions wore seven accelerometers while performing ADL for 30 min. Step events manually annotated from video served as ground truth. An optimal sensing and analysis configuration for machine learning algorithm development (sensor location, filter range, window length, and regressor type) was identified and compared to existing algorithms developed for able-bodied individuals. The most accurate configuration includes a waist-worn sensor, a 0.5–3 Hz bandpass filter, a 5 s window, and gradient boosting regression. The corresponding algorithm showed a significantly lower error rate compared to existing algorithms trained on able-bodied data. Notably, all algorithms undercounted steps. This study identified an optimal sensing and analysis configuration for machine learning-based step counting in a neurological population and highlights the limitations of applying able-bodied-trained algorithms. Future research should focus on developing accurate and robust step-counting algorithms tailored to individuals with neurological conditions. Full article
(This article belongs to the Section Wearables)
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18 pages, 4559 KB  
Article
Automating Leaf Area Measurement in Citrus: The Development and Validation of a Python-Based Tool
by Emilio Suarez, Manuel Blaser and Mary Sutton
Appl. Sci. 2025, 15(17), 9750; https://doi.org/10.3390/app15179750 - 5 Sep 2025
Viewed by 992
Abstract
Leaf area is a critical trait in plant physiology and agronomy, yet conventional measurement approaches such as those using ImageJ remain labor-intensive, user-dependent, and difficult to scale for high-throughput phenotyping. To address these limitations, we developed a fully automated, open-source Python tool for [...] Read more.
Leaf area is a critical trait in plant physiology and agronomy, yet conventional measurement approaches such as those using ImageJ remain labor-intensive, user-dependent, and difficult to scale for high-throughput phenotyping. To address these limitations, we developed a fully automated, open-source Python tool for quantifying citrus leaf area from scanned images using multi-mask HSV segmentation, contour-hierarchy filtering, and batch calibration. The tool was validated against ImageJ across 11 citrus cultivars (n = 412 leaves), representing a broad range of leaf sizes and morphologies. Agreement between methods was near perfect, with correlation coefficients exceeding 0.997, mean bias within ±0.14 cm2, and error rates below 2.5%. Bland–Altman analysis confirmed narrow limits of agreement (±0.3 cm2) while scatter plots showed robust performance across both small and large leaves. Importantly, the Python tool successfully handled challenging imaging conditions, including low-contrast leaves and edge-aligned specimens, where ImageJ required manual intervention. Processing efficiency was markedly improved, with the full dataset analyzed in 7 s compared with over 3 h using ImageJ, representing a >1600-fold speed increase. By eliminating manual thresholding and reducing user variability, this tool provides a reliable, efficient, and accessible framework for high-throughput leaf area quantification, advancing reproducibility and scalability in digital phenotyping. Full article
(This article belongs to the Special Issue Artificial Intelligence Applications in Precision Agriculture)
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20 pages, 3873 KB  
Article
Stability Evaluation of Rock Slope–Anchoring Systems Based on Catastrophe Theory
by Peng Xia, Bowen Zeng, Jie Liu, Yiheng Pan and Xiaofeng Ye
Appl. Sci. 2025, 15(17), 9438; https://doi.org/10.3390/app15179438 - 28 Aug 2025
Viewed by 500
Abstract
With the rapid development of China’s economy, the number and scale of infrastructure projects in energy, water conservancy, and transportation have expanded significantly. Anchoring technology has been widely applied, resulting in the formation of numerous rock slope–anchoring systems. This study proposes a novel [...] Read more.
With the rapid development of China’s economy, the number and scale of infrastructure projects in energy, water conservancy, and transportation have expanded significantly. Anchoring technology has been widely applied, resulting in the formation of numerous rock slope–anchoring systems. This study proposes a novel method for evaluating the stability of rock slope–anchoring systems by introducing catastrophe theory into the stability assessment framework. Based on the characteristics of the rock slope–anchoring system and its stability-influencing factors, a hierarchical analytic structure for catastrophe-level evaluation is constructed, and relevant indicator data are collected. Catastrophe models are selected according to the identified state and control variables, and catastrophe levels are computed to establish a sample dataset. The relationship between catastrophe levels and the stability coefficients of rock slope–anchoring systems is verified to define stability grade intervals. Stability evaluation is then performed by calculating the catastrophe level of each system. The results indicate that: (1) the proposed method effectively considers the influence of multiple factors on the stability of rock slope–anchoring systems, ensuring high accuracy in the evaluation. (2) The method allows for the automatic quantification of the relative importance of indicators within the same hierarchy, reducing subjectivity caused by manual weighting. (3) By standardizing state variables and computing catastrophe levels, the method couples qualitative descriptions with mechanical parameters, enhancing the objectivity of the assessment. (4) The stability evaluation method for rock slope–anchorage systems based on mathematical catastrophe theory determines system stability through catastrophe-order analysis, featuring a concise process and clear results. It enables rapid evaluation of the stability of similar rock slope–anchorage systems and offers high efficiency for cluster assessments. Full article
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21 pages, 4640 KB  
Article
Postpartum Uterine Involution in Cows: Quantitative Assessment of Structural Remodeling and Immune Cell Infiltration
by Karine V. Aires, Ana Paula da Silva, Leonardo G. de Andrade, Alexandre Boyer, Gustavo Zamberlam, Valerio M. Portela, Alfredo Q. Antoniazzi and Guillaume St-Jean
Animals 2025, 15(17), 2520; https://doi.org/10.3390/ani15172520 - 27 Aug 2025
Viewed by 1071
Abstract
Postpartum uterine involution in cattle involves complex morphological and immunological changes essential for restoring uterine health and fertility. This study evaluated endometrial biopsies collected at four postpartum time points to characterize tissue remodeling and immune cell dynamics during involution. Histology revealed intact luminal [...] Read more.
Postpartum uterine involution in cattle involves complex morphological and immunological changes essential for restoring uterine health and fertility. This study evaluated endometrial biopsies collected at four postpartum time points to characterize tissue remodeling and immune cell dynamics during involution. Histology revealed intact luminal columnar epithelium in 92.98% of samples, with stable stromal architecture. Stromal edema decreased by Day 7 but increased again by Day 35, while endometrial gland numbers significantly rose at Day 35, suggesting glandular recovery linked to resumed cyclicity. Subepithelial collagen deposition peaked on Day 21, indicating active extracellular matrix remodeling. Immunologically, early postpartum was marked by increased PMNs and macrophages, whereas Day 21 showed peak infiltration of natural killer (NK) cells and T and B lymphocytes, sometimes forming lymphoid aggregates. Manual and automated immune cell quantifications correlated well. These findings demonstrate a dynamic shift from acute neutrophil-dominated inflammation to a lymphocyte-rich environment during uterine involution. This immune modulation may contribute to the earlier diagnosis of subclinical endometritis, typically identified at later stages of postpartum period. Overall, this study provides insight into the temporal immunomorphological events supporting uterine recovery, with potential implications for reproductive management in dairy cattle. Full article
(This article belongs to the Special Issue Uterine Homeostasis and Disease in Dairy Cows)
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12 pages, 962 KB  
Article
Automated Single-Cell Analysis in the Liquid Biopsy of Breast Cancer
by Stephanie N. Shishido, George Courcoubetis, Peter Kuhn and Jeremy Mason
Cancers 2025, 17(17), 2779; https://doi.org/10.3390/cancers17172779 - 26 Aug 2025
Viewed by 714
Abstract
Background/Objectives: Breast cancer (BC) is the most prevalent cancer worldwide, with approximately 40% of early-stage BC patients developing recurrence despite initial treatments. Current diagnostic methods, such as mammography and solid tissue biopsies, face limitations in sensitivity, accessibility, and the ability to characterize [...] Read more.
Background/Objectives: Breast cancer (BC) is the most prevalent cancer worldwide, with approximately 40% of early-stage BC patients developing recurrence despite initial treatments. Current diagnostic methods, such as mammography and solid tissue biopsies, face limitations in sensitivity, accessibility, and the ability to characterize tumor heterogeneity or monitor systemic disease progression. Methods: To address these gaps, this study investigates a fully automated analysis workflow using data derived from fluorescent Whole-Slide Imaging (fWSI) for detecting and classifying rare cells (circulating tumor and tumor microenvironment cells) in peripheral blood samples. Our methodology integrates supervised machine learning algorithms for rare event detection, immunofluorescence-based classification, and statistical quantification of cellular features. Results: Using a fWSI dataset of 534 cancer and non-cancer peripheral blood samples, the automated model demonstrated high concordance with manual annotation, achieving up to 98.9% accuracy and a precision-sensitivity AUC of 83.2%. Morphometric analysis of rare cells identified significant differences between normal donors, early-stage BC, and late-stage BC cohorts, with distinct clusters emerging in late-stage BC. Conclusions: These findings highlight the potential of liquid biopsy and algorithmic approaches for improving BC diagnostics and staging, offering a scalable, minimally invasive solution to enhance clinical decision-making. Future work aims to refine the automated framework to minimize errors and improve the robustness across diverse cohorts. Full article
(This article belongs to the Section Cancer Causes, Screening and Diagnosis)
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