Search Results (212)

Metabolic syndrome (MetS) is defined as a group of metabolic defects that include hypertension, insulin resistance, visceral obesity, fatty liver disease, and atherosclerotic cardiovascular disease (CVD). The first step in controlling the progression of MetS is lifestyle changes, including dietary modification. Regular consumption of fruits, vegetables, whole grains and other plant foods negatively correlates with the risk of developing chronic diseases. Green leafy vegetables (GLVs) are a key element of healthy eating habits and an important source of vitamins C and E, carotenoids—mainly β-carotene and lutein—and minerals. This review discusses and summarizes the current knowledge on the health benefits of consuming GLVs in the prevention and treatment of MetS to provide a compendium for other researchers investigating new natural products. Full article

The effect of white and additional red and blue LED lighting at night (Blue-NLL, Red-NLL) and during the pre-harvest period (Blue-P-hLL, Red-P-hLL) on morphological and physiological parameters, elemental composition, content of polyphenols, and essential oils of purple basil cultivars ‘Ararat’ and green basil ‘Tonus’ grown in the hydroponic conditions of the climatic chamber was studied. The height of the plants was determined by the variety and the LED irradiation period. The highest purple basil plants were obtained in the variant with Blue-NLL illumination; the highest green basil plants were obtained under Blue-P-hLL and Red-P-hLL. The red spectrum, regardless of the lighting period and variety, increased the area and number of leaves, biomass, and vegetative productivity. Significant changes in the elemental composition of the vegetative mass of basil varieties were determined by the period of exposure to the red spectrum. Red-P-hLL stimulated the absorption and accumulation of Mg, Ca, S, and P from the nutrient solution, and Red-P-hLL reduced the nitrate content by more than 30.00%. Blue-NLL lighting increased the content of quercetin, rosmarinic acid, and essential oil and reduced the nitrate content in the vegetative mass by more than 40.00%. The effectiveness of the white LED was observed in increasing the vegetative mass of ‘Tonus’. The results of this study will be in demand in the real sector of the economy when improving resource-saving technologies for growing environmentally friendly leafy vegetable crops with improved chemical composition and high vegetative productivity. Full article

Based on the single-crank linkage mechanism and the double-pendulum rod mechanism, herein, a reciprocating swing single-blade cutting device is appropriately designed for the needs of GLVs’ planting and cutting operations. It can effectively solve the existing double-blade cutting device’s in-soil operation issue, where clods of soil and stones adhere to the upper and lower cutting blades, resulting in problems such as excessive wear and blade fracture. Using ADAMS, a virtual model of the cutting device is established, and a kinematic analysis of the cutting process is performed to accurately determine the cutting trajectory curve and the mathematical model of the cutting rate. The single-factor test and quadratic regression orthogonal combination test are designed to investigate the influence of the test factors, including crank length, crank rotation speed, and forward speed on the repeated-cutting rate and the miss-cutting rate. Comprehensively considering the stability and reliability of cutting, power consumption, device design, and processing difficulty, the optimal operating parameters of the cutting device are obtained as crank length 19 mm, crank rotation speed 650 r/min, and forward speed 0.5 km/h. On this basis, the fitted regression equations of the repeated-cutting rate and the miss-cutting rate are established, the miss-cutting rate and the repeated-cutting rate under the optimal parameters are 1.519% and 28.503%, and the corresponding errors with the simulation values are obtained as 11.36% and −0.45%, respectively, which verified the validity of the fitted regression equations. In the present investigation, the motion behavior of the single-blade cutting device is methodically examined for the first time, and the cutting motion rules of the cutter are illustrated. The research results aim to provide a fairly solid theoretical basis and practical reference for the optimization design of the in-soil cutting device of GLVs’ orderly harvester. Full article

Indoor farming presents a sustainable response to urbanization and climate change, yet optimizing light use efficiency (LUE) remains vital for maximizing crop yield and minimizing energy use. This study introduces an IoT-based framework for adaptive light management in controlled environments, using lettuce (Lactuca sativa L.) as a model crop due to its rapid growth and sensitivity to light spectra. The system integrates advanced LED lighting, real-time sensors, and cloud-based analytics to enhance light distribution and automate adjustments based on growth stages. The key findings indicate a 20% increase in energy efficiency and a 15% improvement in lettuce growth compared to traditional static models. Novel metrics—Light Use Efficiency at Growth stage Canopy Level (LUEP) and Lamp Level (LUEL)—were developed to assess system performance comprehensively. Simulations identified optimal growth conditions, including a light intensity of 350–400 µmol/m²/s and photoperiods of 16–17 h/day. Spectral optimization showed that a balanced blue-red light mix benefits vegetative growth, while higher red content supports flowering. The framework’s feedback control ensures rapid (<2 s) and accurate (>97%) adjustments to environmental deviations, maintaining ideal conditions throughout growth stages. Comparative analysis confirms the adaptive system’s superiority over static models in responding to dynamic environmental conditions and improving performance metrics like LUEP and LUEL. Practical recommendations include stage-specific guidelines for light spectrum, intensity, and duration to enhance both energy efficiency and crop productivity. While tailored to lettuce, the modular system design allows for adaptation to a variety of leafy greens and other crops with species-specific calibration. This research demonstrates the potential of IoT-driven adaptive lighting systems to advance precision agriculture in indoor environments, offering scalable, energy-efficient solutions for sustainable food production. Full article

Background: Equally with other indigenous green leafy vegetables, Solunum nigrum L. has been widely consumed by the VhaVenda tribe found in the Limpopo Province of South Africa since ancient times as a source of food diversification due to its higher-quality nutritional value, sustainability, food security, and medicinal benefits. It is mostly cultivated from seeds in seedling trays and transplanted in the open field, and at the maturity stage, marketing and distribution are mainly conducting through informal markets (i.e., street vendors). However, recently, it can be found in selected supermarkets and commercial grocery stores in South Africa. The leaves and young shoots of S. nigrum are cooked solely and/or as a supplementary vegetable with Brassica rapa L. subsp. chinensis (Chinese cabbage), Spinacia oleracea L. (spinach), Amaranthus graecizans L. (green amaranth), Solanum lycopersicum L. (tomato), and/or cooking oil for flavor. Objective: Contrary to other green leafy vegetables, few studies have been conducted on the metabolites released by S. nigrum and the influence of growing conditions on the metabolites thereof. Method: A ¹H-nuclear magnetic resonance tool was used to identify the untargeted metabolites released by S. nigrum, and spectra were phase-corrected and binned with MestReNova and statistically analyzed with SIMCA 18.0.2. Results: The findings showed that a total of 12 metabolites were detected between the growing conditions. Eleven similar metabolites, such as glycocholate, chlorogenate (human health benefits), caffeine for its bitter taste, choline, 3-Chlorotyrosine (antidiabetic, blood pressure), etc., and a few vital soluble sugars, were detected in S. nigrum samples grown in the open field and greenhouse-cultivated. Glucose was exclusively detected in the S. nigrum grown under greenhouse conditions. Full article

Scallions are a highly valued leafy vegetable and are enjoyed worldwide due to their appealing taste and nutritional benefits. A combination of short cultivation cycles and high market demand not only enhances food security but also offers a profitable opportunity for growers. In our study, we aim to evaluate the effect of increasing boron (B) applications, specifically 0, 0.2, 0.4, 0.8, 1.2, and 1.6 mM B supplied as boric acid (H₃BO₃) in the nutrient solution, on several key physiological and agronomic parameters in scallions. Results showed that the effects of increasing B levels on biomass production were insignificant, but the root fresh weight (FW) significantly decreased with all B levels. Higher B levels (1.2 and 1.6 mM) caused decreases of 22.9% and 29.6%, respectively. The effects of all B levels on photosynthetic pigment contents [chlorophyll (Chl) a, b, a + b, and carotenoid (Car)], root and shoot membrane permeability (MP), and root, shoot, and leaf nutritional status [phosphorus (P), potassium (K), calcium (Ca), and sodium (Na) concentrations] were found insignificantly. However, all B levels caused a significant increase in the B concentrations of the root, shoot, and leaf of scallions and plants translocated the majority of applied B into their leaves. The translocation factor (TF) of B from the root to the leaf was found to be 138.2%, 133.3%, and 107.3% with 0.8, 1.2, and 1.6 mM B levels, respectively. Moreover, plants exposed to high levels of B showed no significant response or toxicity symptoms. We concluded that B is a phloem mobile element in onion, a non-graminaceous monocotyledonous plant, and therefore accumulates in the upper organs but illustrates partial toxicity symptoms in leaves. Studies with higher B concentrations could be recommended to determine critical B levels for green onion production in B-contaminated areas. Full article

This study investigated the accumulation of nitrate and nitrite, as well as the vitamin C content, in various leafy vegetables, including amaranth greens, katuk, morning glory, squash leaves, vine spinach, leaf lettuce, romaine lettuce, Vietnamese basil, Vietnamese perilla, komatsuna, leeks, and spinach, grown with either organic or synthetic fertilizers. The findings indicate that the type of fertilizer significantly influences nitrate accumulation and vitamin C content in these vegetables. Organic fertilizers were found to reduce nitrate levels and increase vitamin C content in amaranth greens, katuk, morning glory, squash leaves, vine spinach, leaf lettuce, Vietnamese basil, Vietnamese perilla, and spinach compared to the results for synthetic fertilizers. However, high nitrate concentrations in leaf lettuce, komatsuna, and spinach pose potential health risks. The study also identified elevated nitrate levels in vegetables that are not currently regulated. Furthermore, more than half of the samples contained nitrite, for which no maximum permissible level has been established. These findings underscore the importance of organic vegetable cultivation in enhancing both human health and environmental sustainability. Full article

Leafy greens injuries occur from farm to table, causing leakage of cellular contents that promote the multiplication of foodborne pathogens and impose oxidative stress. Fresh beverages made from blended uncooked fruit and vegetables have become a popular food. The effect of cellular contents of different leafy greens on the multiplication of the important pathogen Escherichia coli O157:H7 (EcO157) under temperature abuse was investigated. Leafy greens consisted of spinach and different lettuce types (romaine, iceberg, butterhead, green leaf, and red leaf). Fructose, glucose, and sucrose concentrations in the leaves were quantified by HPLC. H₂O₂ concentration was measured via a peroxidase-based assay. Young leaves of iceberg, romaine, and green leaf lettuce held significantly greater total amounts of the three carbohydrates than middle-aged leaves. Except for iceberg and red leaf lettuce, all middle-aged leaves contained greater H₂O₂ than young leaves. EcO157 density change in leaf contents over 5 h incubation related neither to individual nor total carbohydrate concentration but was negatively associated with H₂O₂ concentration (regression analysis; p < 0.05). Given the common use of antioxidants to maintain the organoleptic aspects of homogenized produce beverages and certain fresh-cut produce, the antimicrobial effect of reactive oxygen species may be important to preserve in ensuring their microbial safety. Full article

Light is a key factor influencing the growth and quality of crops in plant factories. To explore the optimal light quality for pakchoi production, five light formulations were applied to ‘Youguan NO.3’ pakchoi: white LEDs (W; CK); white/red = 4:1 (WR); white/blue = 4:1 (WB); white/red/blue = 3:1:1 (WRB); and white/green = 4:1 (WG), all with a light intensity of 250 ± 10 µmol·m⁻²·s⁻¹. The results showed significant variations in growth indices, nutritional quality, enzyme activity, and other parameters under different light qualities. The best growth results were observed under the WRB treatment. Chloroplasts under WRB treatment appeared well-developed, with clear grana lamellae. The thylakoids in the chloroplast grana of the WRB plants were densely stacked, and a large number of starch grains were detected. The contents of total sugar, soluble sugar, soluble protein, and protein nitrogen were significantly higher under the WB, WRB, and WR treatments compared to the CK treatment, along with a significant reduction in nitrate content. Among all the treatments, WRB treatment resulted in the highest levels of total sugar, starch, free amino acids, soluble protein, total nitrogen, protein nitrogen, and ascorbic acid (AsA). Enzyme activity assays revealed that the activities of sucrose phosphate synthetase (SPS), nitrate reductase (NR), glutamine synthetase (GS), glutamate synthetase (GOGAT), and glutamate dehydrogenase (GDH) were highest under WRB treatment. Therefore, supplemental red-blue mixed light can effectively improve the growth and nutritional properties of pakchoi grown under white light. This supplementary lighting strategy provides a new way to enhance the nutritional value of leafy vegetables in plant factories. Full article

Quinones, one of the oldest organic compounds, are of increasing interest due to their abundant presence in a wide range of natural sources and their remarkable biological activity. These compounds occur naturally in green leafy vegetables, fruits, herbs, animal and marine sources, and fermented products, and have demonstrated promising potential for use in health interventions, particularly in the prevention and management of type 2 diabetes (T2DM). This review aims to investigate the potential of quinones as a health intervention for T2DM from the multidimensional perspective of their sources, types, structure–activity relationship, glucose-lowering mechanism, toxicity reduction, and bioavailability enhancement. Emerging research highlights the hypoglycemic activities of quinones, mainly driven by their redox properties, which lead to covalent binding, and their structural substituent specificity, which leads to their non-covalent binding to biocomplexes. Quinones can improve insulin resistance and regulate glucose homeostasis by modulating mitochondrial function, inflammation, lipid profile, gastrointestinal absorption, and by acting as insulin mimetics. Meanwhile, increasing attention is being given to research focused on mitigating the toxicity of quinones during administration and enhancing their bioavailability. This review offers a critical foundation for the development of quinone-based health therapies and functional foods aimed at diabetes management. Full article

The bunching onion is an important leafy vegetable, prized for its distinctive flavor and color. It is consumed year-round in Japan, where a stable supply is essential. However, in recent years, the challenges posed by climate change and global warming have resulted in adverse effects on bunching onions, including stunted growth, discoloration, and the development of leaf tipburn, threatening both crop quality and yield. Furthermore, as bunching onion belongs to the Allium genus, which includes globally significant vegetables such as onion and garlic, studying the impact of climate change on bunching onion serves as an ideal model. The insights gained can also be applied to other crops and regions. This study investigates the effects of different summer growth conditions on the metabolite profile of heat-tolerant bunching onions with dark green leaf blade coloration and examines their association with leaf tipburn. Pigment compound quantification, functional component analysis, leaf tipburn rate assessment, and widely targeted metabolome profiling were performed across two commercial ${\mathrm{F}}_1$ varieties, one purebred variety, and six Yamaguchi Prefecture-bred ${\mathrm{F}}_1$ lines under different growing conditions. The results obtained were subjected to comparative analyses based on the varieties and groups classified by high and low leaf tipburn rates. The results revealed that $\beta$-carotene accumulation peaked with May sowing and July harvest, while the highest accumulation of other pigment compounds was observed with May sowing and September harvest. Additionally, metabolome analysis related to leaf tipburn rates identified several organosulfur compounds, with gamma-glutamyl-propenyl cysteine sulfoxide emerging as one of the key compounds. Based on the intensity data, the fold change of this metabolite was calculated to be 1.66, indicating an increase in the leaf tipburn group compared to the control group. In the control groups, organosulfur compounds appeared to undergo turnover in preparation for stress response. In contrast, in the leaf tipburn groups, it is hypothesized that organosulfur compounds were converted into precursors of pungency, resulting in inadequate responses to stress. This study aims to elucidate the mechanisms through which organosulfur compounds transition into pungent compounds and to develop varieties with improved resistance to leaf tipburn. Full article

Background: Inorganic nitrate is abundant in leafy green vegetables and has been shown to exert positive cardiovascular effects through nitric oxide-related pathways. The enteral microbiome is an emerging key player in cardiovascular diseases and depends on dietary habits. Whether dietary inorganic nitrate impacts on the microbiome and atherosclerosis-associated microbiome-dependent metabolites like short chain fatty acids (SCFA) and trimethylamine N-oxide (TMAO) is unknown. Methods: In a double-blind randomized controlled trial, 30 healthy volunteers were included who either received dietary nitrate (0.12 mmol/kg bodyweight) or placebo (equimolar amounts of sodium chloride) for 30 days. The microbiome metabolites TMAO and SCFA were analyzed. The enteral microbiome was analyzed by 16S-rRNA sequencing at baseline and follow-up. Results: Systolic blood pressure decreased after nitrate supplementation (baseline 124.73 mmHg vs. follow up 120 mmHg, p < 0.05) with no change in controls. Dietary nitrate supplementation increased TMAO levels (nitrate baseline 349.28 μ/L vs. nitrate follow-up 481.15 μ/L, p < 0.05), while SCFA levels remained unchanged. The relative abundance of Akkermansia and taxa of Clostridiales were higher in individuals with high compared to normal TMAO levels after nitrate supplementation, while Shannon diversity, richness and evenness did not differ between both groups. Conclusions: Our results indicate that dietary nitrate supplementation is associated with alterations to the enteral microbiome with an impact on proatherogenic metabolites. Further work is warranted to investigate the causal relationship between dietary nutrients, the microbiome and downstream metabolites. Full article

Plant factories with artificial lighting (PFALs) are a notable choice for urban agriculture due to the system’s benefits, where light can be manipulated to enhance the product’s yield and quality. Our objective was to test the effect of light spectra with different red-blue combinations and white light on the growth, physiology, and overall quality of three baby-leaf vegetables (green lettuce, kale, and pak choi) grown in a restaurant’s PFAL. Leaf mass per area was lower under the most blue-containing treatments in all species. The performance indices (PI_abs and PI_tot) of the photosynthetic apparatus were lower under more red light with the exception of PI_abs in pak choi. Total soluble solids accumulation was diminished under most of the blue-containing LEDs, while total phenolics and antioxidant activity were induced by red-blue environments rich in blue light. Moreover, chlorophyll and carotenoid accumulation was also enhanced under blue-rich light treatments. Nitrate content was the lowest under monochromatic blue in all species. Finally, the employees were asked about their views on the PFAL within the restaurant’s compounds and they expressed positive opinions. Overall, a light environment including red and blue wavelengths proved beneficial for baby leafy vegetable production in terms of yield and quality. Full article

Spinach (Spinacia oleracea L.) is a dioecious, wind-pollinated, and important green leafy vegetable that is widely cultivated worldwide. The characteristics of spinach pollen remain unclear. Herein, we investigated the pollen microscopic morphology of nine spinach genotypes by scanning electron microscopy (SEM). Because pollen grains of spinach are isodiametric and spheroidal, the following data for the pollen properties were observed: the maximum diameter of the pollen (27.66–33.05 um), pore diameter max (1.26–2.57 um), the number of visible pores (30–44), the density of pore elements/100 μm² (4.33–11.00), and the density of spinule elements/10 μm² (4.00–5.33). These are useful characteristics in distinguishing the species. Furthermore, by applying the cluster analysis method, based on key components, nine varieties are categorized into two main classes. These results provide a useful reference for the classification and identification of different spinach varieties. Full article

Vitamin D is a conditionally essential fat-soluble vitamin found in foods such as fish; fish oil; egg yolks; animal fats; some mushroom varieties; and fortified foods such as cheese, margarine, milk, infant formula, and some ready-to-eat cereals. Calcium (Ca) is found in milk, cheese, canned fish, ready-to-eat cereals, milk substitutes, dark green leafy vegetables, and sports drinks. There are more than fifty metabolites of vitamin D. Vitamin D participates in immune regulation, apoptosis induction, insulin secretion, inflammation, cell differentiation, calcium balance and regulation, bone mineralization, and phosphorus homeostasis. Ca is an essential macro-mineral involved in bone and teeth matrices, strength, and hardness; muscle and cardiovascular movement; neurological messaging; and in the release of hormones. Peer-reviewed journal articles were accessed from the search engine PubMed. The authors reviewed the references in the peer-reviewed journal articles, websites, and review articles if the authors proposed a new theory or mechanism. Vitamin D and Ca have important relationships; therefore, many factors may impede or interfere with the body’s ability to absorb or utilize vitamin D and or Ca and may result in low or excessive levels of each. Additionally, genetic/medically related absorption issues and low intake may also result in deficiencies. This review discusses the introduction of each, their functions, absorption, somatic transportation, the relationship between vitamin D and Ca, and recommendations and supplementation strategies if available. Full article