Search Results (17)

Wood ash, a byproduct of woody biomass power generation, has potential as an alternative K fertilizer due to its high K content and pH-raising properties. However, concerns remain about heavy metal contaminants like Cr and the limited understanding of element dynamics in soil–solution–crop systems after wood ash’s application. This study examined the effects of 1% (w/w) wood ash on element dynamics and komatsuna (Brassica rapa var. perviridis) uptake in low-K soil through a pot experiment. XRD was used to analyze mineral composition, SEM-EDS to observe surface and elemental properties, and XANES to examine Cr speciation in wood ash. Soil solution analysis covered macro- and micronutrients, heavy metals, anions, pH, and DOC, while crop element concentrations and aboveground dry weight were also quantified. The chemical speciation of Cu and Cr in a soil solution was modeled using Visual MINTEQ. Wood ash significantly increased K concentrations (from 17 mg/L to 650 mg/L) in the soil solution, along with Ca, Mg, P, and Mo, while reducing Ni, Mn, Zn, and Cd levels. Komatsuna K uptake surged from 123 mg/kg to 559 mg/kg, leading to a 3.31-fold biomass increase. Notably, the Cd concentration in the crops dropped significantly from 0.709 to 0.057 mg/kg, well below the Codex standard of 0.2 mg/kg. Although Cu and Cr concentrations rose in the soil solution, crop uptake remained low due to >99% complexation with fulvic acid, as confirmed by Visual MINTEQ modeling. This study confirms that wood ash is an effective K fertilizer, but emphasizes the need for risk mitigation strategies to ensure safe and sustainable agricultural application. Full article

This study investigated the accumulation of nitrate and nitrite, as well as the vitamin C content, in various leafy vegetables, including amaranth greens, katuk, morning glory, squash leaves, vine spinach, leaf lettuce, romaine lettuce, Vietnamese basil, Vietnamese perilla, komatsuna, leeks, and spinach, grown with either organic or synthetic fertilizers. The findings indicate that the type of fertilizer significantly influences nitrate accumulation and vitamin C content in these vegetables. Organic fertilizers were found to reduce nitrate levels and increase vitamin C content in amaranth greens, katuk, morning glory, squash leaves, vine spinach, leaf lettuce, Vietnamese basil, Vietnamese perilla, and spinach compared to the results for synthetic fertilizers. However, high nitrate concentrations in leaf lettuce, komatsuna, and spinach pose potential health risks. The study also identified elevated nitrate levels in vegetables that are not currently regulated. Furthermore, more than half of the samples contained nitrite, for which no maximum permissible level has been established. These findings underscore the importance of organic vegetable cultivation in enhancing both human health and environmental sustainability. Full article

Due to widespread selenium deficiency in food the aim of this study was to evaluate the effectiveness of a new Se(II)-containing organic chemical compound 2-iminoselenazolidin-4-ones (ISeA) in the form of a nanoscale associate (1–5 nm) solution for Swiss chard and komatsuna plants biofortification. Application of the chosen substance as a foliar treatment (2 mg·L⁻¹) and as an additive to a hydroponic nutrient solution (10 mg·L⁻¹) was performed. Both cultures had a high level of Se absorption, distribution and accumulation in leaves two or more times greater than in petioles. Se content in chard petioles (15 mg·L⁻¹) when applying ISeA as a component of the nutrient solution exceeded the accumulation of Se during foliar treatment (9.6 mg·L⁻¹) and the same trend in the komatsuna leaves was observed. When applying ISeA to the nutrient solution, an increase in komatsuna and chard biomass was seen at 36 and 68% and for leaf treatment by 21 and 45%, respectively. For komatsuna and chard an increase in the ratio of dry to fresh weight was also observed to be 27 and 26%, and for foliar treatment—0 and $16\%$, respectively. Treatments led to increase in chard plants height (7–17%), enlargement of leaves (19–42%), a rise in photosynthetic pigments (20–60%) and anthocyanin (2.9 and 2.2 times) concentration, and for komatsuna—the multiplication of leaves number ($28\%$) and their surface area (27–29%) as well as a rise in the concentration of anthocyanin (1.0 and 1.6 times) with foliar treatment and nutrient solution enrichment. Full article

Methane fermentation, utilizing food waste (FW), is viewed as a sustainable strategy that leverages garbage and agricultural waste to conserve the environment. However, FW compost encounters growth inhibition issues, which we examine in this study. The aim of this study was to assess the influence of various compost mixtures on seed germination growth and the yield of Komatsuna (Brassica rapa). The experiment employed FW compost mixtures with biochar (BC), clay (CL), weeds (WD), and a control group in a completely randomized design with three replications to monitor germination. The experimental pots, arranged in a complete factorial design, involved three treatment factors: compost type (FW or HM), biochar presence or absence (WB or NB), and three input rates (25 g pot⁻¹, 50 g pot⁻¹, and 100 g pot⁻¹), each in triplicate. The combination of FW and BC exhibited an enhanced germination rate compared to FW alone. Moreover, the inclusion of biochar significantly amplified this effect, particularly at the input rate of 50 g pot⁻¹ and had a substantial impact on the interaction between input rate, compost type, and biochar on variables such as nitrogen (N) uptake, % N, soil carbon, and yield. Homemade BC demonstrates an increasing fertilizer cost performance (FCP) as the input rate rises across all fertilizer combinations, while commercially priced BC exhibits a reverse relationship with FCP. These findings suggest that the addition of biochar enhances the performance of methane fermentation residue compost, thereby promoting plant growth through the processing of environmentally sustainable waste. Full article

The disposal of tea leaves discarded in the tea beverage market and clinker from coal-fired power plants has an impact on the environment; however, there are no reported cases of their combination for composting. Therefore, this study evaluated the effect of adding clinker from a coal-fired power plant to compost based on tea leaves, an organic waste product, on the composting rate and quality. The tea leaves-only compost was designated as Clinker 0%, and composts with 20% (w/w), 40% (w/w), and 60% (w/w) tea leaves supplemented with clinker were designated as Clinker 20, 40, and 60%, respectively. Each mixed material was placed in a 35 L polypropylene container with a lid and allowed to compost for 95 days. The composting rate was evaluated by the chemical oxygen demand (COD) in hot water extract and plant tests using juvenile komatsuna (Brassica rapa var. perviridis). The addition of clinker reduced the COD at the beginning of composting by 52.0, 74.3, and 86.7% in Clinker 20, 40, and 60%, respectively, compared to Clinker 0%. Furthermore, root elongation one month after composting was inhibited by Clinker 0% (60.1% relative to distilled water), but not by the addition of clinker (91.7–102.7% relative to distilled water). This suggests that the addition of clinker to tea leaf compost may accelerate composting. Full article

Mulch has been considered effective for saving water and promoting plant growth. However, little has been investigated about the effect of mulch from recycled shredded paper under different soil moisture conditions on spinach growth. A pot-grown Komatsuna spinach experiment with a factorial design was conducted under two main conditions, with and without shredded paper mulch. Each of these conditions was treated with three different soil moisture levels (SWC) 20%, 25%, and 30%. The smallest evapotranspiration amount was in the mulch-only, no-plant treatment with SWC 20% (92.88 mm), and the largest was in the plant-only, no-mulch treatment (226.19 mm). All biomass yield attributions were negatively influenced by increasing the frequency of irrigation and SWC levels. SWC 20% with shredded mulch resulted in the largest dry matter, although it was statistically non-significant. But it resulted in taller plant heights and a larger leaf area index (LAI) compared to soil with no shredded mulch (p < 0.05). For SWC 25% and 30% treatments, Komatsuna spinach with no shredded mulch resulted in slightly superior plant dry matter and plant height, compared to mulched plants. These results suggest that SWC 20% with shredded paper mulch has the highest potential for saving water among all treatments for growing spinach under limited water availability. Full article

The pyrolysis of biomass material results in pyroligneous acid (PA) and biochar, among other by-products. In agriculture, PA is recognized as an antimicrobial agent, bio-insecticide, and bio-herbicide due to antioxidant activity provided by a variety of constituent materials. Application of PA to crop plants and soil can result in growth promotion, improved soil health, and reduced reliance on polluting chemical crop inputs. More detailed information regarding chemical compound content within PA and identification of optimal chemical profiles for growth promotion in different crop species is essential for application to yield effective results. Additionally, biochar and PA are often applied in tandem for increased agricultural benefits, but little is known regarding the optimal proportion of each crop input. This work reports on the effect of combined applications of different proportions of PA (200- and 800-fold dilutions) and chemical fertilizer rates (100%, 75%, 50%, and 0%) in the presence or absence of biochar on Komatsuna (Brassica rapa var. perviridis, Japanese mustard spinach) plant growth. To elucidate the chemical composition of the applied PA, four different spectroscopic measurements of fluorescence excitation were utilized for analysis—excitation-emission matrix, ion chromatography, high-performance liquid chromatography, and gas chromatography-mass spectrometry. It was determined that PA originating from pyrolysis of Japanese pine wood contained different classes of biostimulants (e.g., tryptophan, humic acid, and fulvic acid), and application to Komatsuna plants resulted in increased growth when applied alone, and in different combinations with the other two inputs. Additionally, application of biochar and PA at the higher dilution rate increased leaf accumulation of nutrients, calcium, and phosphorus. These effects reveal that PA and biochar are promising materials for sustainable crop production. Full article

Rhodobacter sphaeroides, a purple non-sulfur photosynthetic bacterium (PNSB), was disrupted by sonication and fractionated by centrifugation into the supernatant and pellet. The effects of the supernatant and pellet on plant growth were examined using Brassica rapa var. perviridis (komatsuna) in the pot experiments. Both fractions showed growth-promoting effects: the supernatant at high concentrations (1 × 10⁷ to 4 × 10⁷ cfu-equivalent mL⁻¹) and the pellet at a low concentration of 2 × 10³ cfu-equivalent mL⁻¹). We expected lipopolysaccharide (LPS) to be the active principle of the pellet fraction and examined the effects of LPS on the growth of B. rapa var. perviridis. The growth of the plants was significantly enhanced by the foliar feeding of R. sphaeroides LPS at concentrations ranging from 10 to 100 pg mL⁻¹. The present study is the first report indicating that LPS acts as one of the active principles of the plant-growth-promoting effect of PNSB. Full article

One suitable solution to achieve sustainable development goals (SDGs) is to utilize methane fermentation residue obtained from food waste. However, methane fermentation residue compost is often difficult to use due to its inhibitory properties and pungent smell. To evaluate chemical parameters and plant growth, we examined the use of methane-fermented food waste residue compost (FWM), three types of animal manure, namely, horse manure (HM), cow manure (CWM), and chicken manure (CKM). Our results revealed that mixing food waste compost with cow and horse manure reduced the inhibition of Komatsuna germination, however, chicken manure blending limited inhibition reduction. The improvement of GI in the combination of FWM and animal manure was obtained at a ratio 1:4 with an improvement in GI of 20.8%, 16.8%, and 3.8% for combination FWM + HM, FWM + CWM, and FWM + CKM, respectively. The ratio of the combination of FWM + HM could be increased to a ratio of 2:3 with an improvement of 14.7%. Additionally, for Komatsuna growth, FWM with cow and horse manure mixer considerably enhanced plant growth and yield. Furthermore, the combination of FWM + HM and FWM + CWM could improve the ratio of NUE compost/NUE FWM with the values being 12.0 and 11.1, respectively. Therefore, combining FWM with CWM and HM increases the utility value of methane fermentation residues obtained from food waste as compost for maintaining soil fertility, while reducing the use of chemical fertilizer. Full article

Flowering time is important for Brassica rapa vegetables because premature bolting before harvest can lower yield and quality. FLOWERING LOCUS C (FLC) acts as a key repressor of flowering. In this study, we identified a nonsynonymous mutation at the 58th nucleotide of exon1 in BrFLC1 (named as Pe1+58 (A/C)) by screening resequencing data of 199 B. rapa accessions and verified this mutation as being related to flowering time variation. Strong linkage inheritance was detected between this locus and a previously reported splicing site mutation at intron 6 of BrFLC1 (Pi6+1 (G/A)), showing as co-occurrence of BrFLC1Pe1+58(A) and BrFLC1Pi6+1(G), named as haplotype H1: AG, or co-occurrence of BrFLC1Pe1+58(C) and BrFLC1Pi6+1(A), named as haplotype H2: CA. The frequency distribution of BrFLC1 haplotypes skewed to the haplotype H1 in turnip, broccoletto, mizuna, komatsuna, and taicai, while it was skewed to the haplotype H2 in caixin, pak choi, zicaitai, and wutacai. The frequencies of the two haplotypes were comparable in Chinese cabbage. This indicated that BrFLC1 haplotypes were related to B. rapa intraspecific diversification. Further analysis of a Chinese cabbage collection revealed that accessions from the spring ecotype preferred to keep H1: AG and almost all accessions from the summer ecotype were H2: CA. The early flowering haplotype of BrFLC1 was purified in summer Chinese cabbage, indicating that BrFLC1 had been strongly selected during genetic improvement of summer Chinese cabbages. A significant difference in flowering time of F₂ individuals with the homologous BrFLC1Pi6+1(G) allele but different BrFLC1Pe1+58 (A/C) alleles, indicated that this locus had independent genetic effects on flowering time. The newly identified allelic diversity of BrFLC1 can be used for breeding of resistance to premature bolting in B. rapa vegetables. Full article

In vegetables of Brassica rapa L., Fusarium oxysporum f. sp. rapae (For) or F. oxysporum f. sp. conglutinans (Foc) cause Fusarium yellows. A resistance gene against Foc (FocBr1) has been identified, and deletion of this gene results in susceptibility (focbr1-1). In contrast, a resistance gene against For has not been identified. Inoculation tests showed that lines resistant to Foc were also resistant to For, and lines susceptible to Foc were susceptible to For. However, prediction of disease resistance by a dominant DNA marker on FocBr1 (Bra012688m) was not associated with disease resistance of For in some komatsuna lines using an inoculation test. QTL-seq using four F₂ populations derived from For susceptible and resistant lines showed one causative locus on chromosome A03, which covers FocBr1. Comparison of the amino acid sequence of FocBr1 between susceptible and resistant alleles (FocBr1 and FocBo1) showed that six amino acid differences were specific to susceptible lines. The presence and absence of FocBr1 is consistent with For resistance in F₂ populations. These results indicate that FocBr1 is essential for For resistance, and changed amino acid sequences result in susceptibility to For. This susceptible allele is termed focbr1-2, and a new DNA marker (focbr1-2m) for detection of the focbr1-2 allele was developed. Full article

Microgreens constitute novel gastronomic ingredients that combine visual, kinesthetic and bioactive qualities. The definition of the optimal developmental stage for harvesting microgreens remains fluid. Their superior phytochemical content against mature leaves underpins the current hypothesis of significant changes in compositional profile during the brief interval of ontogeny from the appearance of the first (S1) to the second true leaf (S2). Microgreens of four brassicaceous genotypes (Komatsuna, Mibuna, Mizuna and Pak Choi) grown under controlled conditions and harvested at S1 and S2 were appraised for fresh and dry yield traits. They were further analyzed for macro- and micromineral content using inductively coupled plasma optical emission spectrometry (ICP-OES), carotenoid content using high-performance liquid chromatography with a diode-array detector (HPLC-DAD), volatile organic compounds using solid-phase microextraction followed by gas chromatography-mass spectrometry (SPME-GC/MS), anthocyanins and polyphenols using liquid chromatography-high resolution-tandem mass spectrometry (LC-MS/MS) with Orbitrap technology and for chlorophyll and ascorbate concentrations, well as antioxidant capacity by spectrophotometry. Analysis of compositional profiles revealed genotype as the principal source of variation for all constituents. The response of mineral and phytochemical composition and of antioxidant capacity to the growth stage was limited and largely genotype-dependent. It is, therefore, questionable whether delaying harvest from S1 to S2 would significantly improve the bioactive value of microgreens while the cost-benefit analysis for this decision must be genotype-specific. Finally, the lower-yielding genotypes (Mizuna and Pak Choi) registered higher relative increase in fresh yield between S1 and S2, compared to the faster-growing and higher-yielding genotypes. Although the optimal harvest stage for specific genotypes must be determined considering the increase in yield against reduction in crop turnover, harvesting at S2 seems advisable for the lower-yielding genotypes. Full article

Phenotyping involves the quantitative assessment of the anatomical, biochemical, and physiological plant traits. Natural plant growth cycles can be extremely slow, hindering the experimental processes of phenotyping. Deep learning offers a great deal of support for automating and addressing key plant phenotyping research issues. Machine learning-based high-throughput phenotyping is a potential solution to the phenotyping bottleneck, promising to accelerate the experimental cycles within phenomic research. This research presents a study of deep networks’ potential to predict plants’ expected growth, by generating segmentation masks of root and shoot systems into the future. We adapt an existing generative adversarial predictive network into this new domain. The results show an efficient plant leaf and root segmentation network that provides predictive segmentation of what a leaf and root system will look like at a future time, based on time-series data of plant growth. We present benchmark results on two public datasets of Arabidopsis (A. thaliana) and Brassica rapa (Komatsuna) plants. The experimental results show strong performance, and the capability of proposed methods to match expert annotation. The proposed method is highly adaptable, trainable (transfer learning/domain adaptation) on different plant species and mutations. Full article

Prohydrojasmon (PDJ) can improve the polyphenol and anthocyanin content and antioxidant activity of some crop plants, but it also shows a suppressive effect on the plant growth. This study aimed to investigate the inhibitory effect of PDJ on the growth of two crop plants: komatsuna (Brassica rapa var. periviridis) and eggplant (Solanum melongena). We applied various concentrations of PDJ drip-wise or by spraying to eggplant and komatsuna seedlings and made detailed observations of growth. In general, no significant suppressive effect of PDJ was observed in the aerial parts in both plants. However, a significant inhibitory effect was found in roots treated with PDJ at concentrations of 600 and 1000 ppm. Interestingly, komatsuna treated with PDJ at a concentration of 200 ppm in both approaches resulted in a significant increase in root weight up to 37%. At a concentration range of 200–400 ppm, PDJ showed no inhibitory effects, and in some cases slightly promoted root growth. Therefore, this could be the recommended concentration range. We conclude that application of PDJ can still be beneficial to the vegetable crops without causing serious inhibition or suppression effects on the growth, as long as it is kept at rather low concentrations. Full article

The residue generated by the black soldier fly (Hermetia illucens, BSF) during the processing of organic waste is considered a suitable crop fertilizer. However, no detailed studies have investigated the fertilizer value of the residue obtained from processing household organic waste. In this study, experimental household organic waste (EHOW) was processed by BSF at 200 mg of EHOW per head for 15 days at 27 °C. To evaluate the fertilizer value of the obtained BSF larvae production residue (BSFR), the chemical composition and microbiota were analyzed, and Komatsuna (Brassica rapa var. perviridis) cultivation tests were conducted. BSFR results demonstrated higher ammonium nitrogen and lower nitrate nitrogen, and the highest above-ground dry matter weight of Komatsuna. Although the relative abundance of Escherichia was low, the relative abundance of Xanthomonadaceae, which contains a genus that causes disease in plants, was high. Therefore, the presence of plant pathogens in the BSFR microbiota should be considered. Finally, the effects of BSFR on the external environment requires more detailed investigation. Full article