Search Results (150)

Background: Postmortem DNA identification of highly decomposed human remains is often limited by the availability and quality of conventional biological samples. Keratinized tissues, such as fingernails, represent a potentially valuable alternative due to their anatomical resistance to environmental degradation, however, their use as primary biological material for DNA profiling remains underreported in forensic practice. Case presentation: We report a case involving the recovery of a highly decomposed body of a missing woman, in which DNA samples were collected from a fingernail and a tooth. DNA extraction was performed using the PrepFiler Forensic DNA Extraction Kit for the fingernail sample and PrepFiler BTA Forensic DNA Extraction Kit for the tooth sample. No usable DNA profile was obtained from the tooth sample; however, the fingernail sample yielded a complete and high-quality STR profile with successful amplification across all 24 loci (GlobalFiler PCR Amplification Kit). Reference buccal swabs from the presumed biological parents were collected for subsequent kinship analysis. Discussion: Kinship analysis based on allele frequencies in the Croatian population resulted in a combined paternity index (CPI) corresponding to a probability of paternity of 99.99999812%, providing strong genetic support for the proposed identity of the deceased. Notably, this is the first documented forensic case in Croatia in which nail material served as the primary—and ultimately successful—biological sample for postmortem identification. Conclusions: This case highlights the evidentiary value of fingernails as a robust, accessible, and forensically valid DNA source in postmortem identification, particularly in cases of advanced decomposition where conventional biological materials are unavailable or degraded. Further studies involving larger sample sets and diverse postmortem conditions are needed to support the broader implementation of nail material in routine forensic identification workflows, particularly within the Croatian medico-legal context. Full article

This study investigates the configurational pathways enabling women in Oman to translate entrepreneurial intentions into technology venture creation. By integrating institutional theory and resource-based view, we develop a novel framework examining how formal institutional support (FIS), informal institutional support (IIS), and digital self-efficacy (DSE) interact in Oman’s conservative context. We emphasize the significant enabling role of work–life balance resources (WLBR) and the cultural legitimacy of spousal endorsement. Our mixed-methods design utilizes survey data from 418 female IT graduates and 20 semi-structured interviews, analyzed through fuzzy-set Qualitative Comparative Analysis (fsQCA). The findings indicate that FIS predicts entrepreneurial intention (β = 0.34, p < 0.001) but not venture creation (OR = 0.85, p = 0.298), revealing a visibility gap in policy implementation. IIS predicts venture creation (OR = 1.43, p = 0.033), with spousal endorsement acting as a cultural legitimacy signal. DSE alone fails to predict venture creation but is vital when combined with WLBR. FsQCA identifies a sufficient configuration pathway characterized by the combination of spousal endorsement, domestic support, DSE, and WLBR with solution consistency of 0.93 and coverage of 0.78. WLBR is a necessary condition with necessity consistency of 0.96, demonstrating that venture creation is improbable without it. Qualitative evidence shows founders reposition conservative norms as legitimacy signals, while non-founders emphasize funding barriers despite policy awareness. We recommend that policymakers subsidize care infrastructure, leverage women-led community networks for targeted outreach, and formalize state-backed legitimacy programs that reduce kinship dependency while building autonomy-focused alternatives. Full article

Genetic diversity and kinship information of cotton germplasm resources are fundamental to breeding, providing a theoretical basis for the rational selection of hybrid parents and further breeding of new varieties with high yield, high quality, and multi-resistance. This study utilized cotton varieties that have been used for variety improvement or are widely planted in the Northern Xinjiang cotton region as materials. Genotyping was performed using the ZJU CottonSNP40K chip to analyze genetic diversity and kinship relationships. A total of 26,852 high-quality SNP markers were obtained, including 15,222 SNPs in subgenome A and 11,630 SNPs in subgenome D. The number of SNPs per chromosome ranged from 547 (A04) to 2168 (A08). Based on phylogenetic tree and principal component analysis, the 83 materials were clustered into 3 major subgroups. Group I contained varieties introduced from the former Soviet Union and the United States, which have become important parents for cotton breeding in Northern Xinjiang. Among them, as many as 27 varieties were derived and selected from the introduced US variety ‘Beiersinuo’ as a parent. While playing an important role in cotton breeding in Northern Xinjiang, this has also led to the current situation where the genetic base of Northern Xinjiang varieties is relatively narrow (average kinship coefficient 0.72). It clarifies the significant role of introduced American variety ‘Beiersinuo’ in the breeding of Northern Xinjiang cultivars and provides theoretical guidance for broadening the genetic base of Northern Xinjiang cotton varieties. Full article

First Nations children remain dramatically over-represented in Australia’s Out-of-Home Care (OOHC) system, particularly in New South Wales (NSW), which continues to report the highest numbers nationally. This narrative review, grounded in a relational First Nations Standpoint Theory and decolonising research paradigms, to critically examine the systemic, structural, and historical factors contributing to these disproportionalities. Drawing on interdisciplinary evidence across law, criminology, education, health, governance studies, and public policy, the analysis centres Indigenous-authored scholarship and contemporary empirical literature, including grey literature, inquiries, and community-led reports. Findings reveal that the OOHC system reproduces the colonial logics that historically drove the Stolen Generations. Macro-level structural drivers—including systemic racism, Indigenous data injustice, entrenched poverty and deprivation, intergenerational trauma, and Westernised governance frameworks—continue to shape child protection policies and practices. Micro-level drivers such as parental supports, mental health distress, substance misuse, family violence, and the criminalisation of children in care (“crossover children”) must be understood as direct consequences of structural inequality rather than as isolated individual risk factors. Current placement and permanency orders in NSW further compound cultural disconnection, with ongoing failures to implement the Aboriginal and Torres Strait Islander Child Placement Principle (ATSICPP). Contemporary cultural rights and Indigenous Cultural and Intellectual Property (ICIP) frameworks highlight the urgency of restoring Indigenous authority in decision-making processes. The literature consistently demonstrates that cultural continuity, kinship networks, and ACCO-led models are sort to produce stronger long-term outcomes for children. The review concludes that genuine transformation requires a systemic shift toward Indigenous-led governance, community-controlled service delivery, data sovereignty, and legislative reform that embeds cultural rights and self-determination. Without acknowledging the structural drivers and redistributing genuine power and authority, the state risks perpetuating a cycle of removal that mirrors earlier assimilationist policies. Strengthening First Peoples governance and cultural authority is therefore essential to creating pathways for First Nations children to live safely, remain connected to family and kin, and thrive in culture. Full article

Winterberry (Ilex verticillata) is a deciduous shrub within the Aquifoliaceae family that holds significant ornamental and medicinal value. However, the lack of systematic research on the genetic background and phylogenetic relationships among its cultivars has hindered germplasm conservation and breeding efforts. This study marks the first application of genotyping-by-sequencing (GBS) technology to analyze winterberry germplasm resources. Sequencing was performed on 79 samples from eight representative cultivars, and 3,411,968 high-quality single-nucleotide polymorphism (SNP) markers were developed using a de novo assembly strategy. Population structure analysis based on STRUCTURE indicated K = 8 as the statistically optimal number of genetic components according to the delta K statistic. However, when STRUCTURE results were interpreted together with principal component analysis (PCA) and phylogenetic reconstruction, the winterberry cultivars were consistently summarized into five major genetic clusters. Ilex verticillata ‘Winter Gold’ and I. verticillata ‘Winter Red’ shared highly consistent genetic backgrounds, indicating extremely close kinship; I. verticillata ‘Citronella’ and I. verticillata ‘Oosterwijk’ clustered closely together; I. verticillata ‘Red Sprite’ (Rizhao) and I. verticillata ‘Red Sprite’ (Dezhou), despite differing geographical origins, clustered together, demonstrating good genetic stability; and I. verticillata ‘Golden Verboom’ and I. verticillata ‘Little Goblin Red’ each formed independent genetic branches, possessing unique genetic backgrounds. This study concludes that GBS effectively reveals the complex genetic structure among winterberry cultivars. The findings not only provide accurate molecular evidence for cultivar identification and intellectual property protection but also lay a solid foundation for future hybrid breeding, including parent selection, identification of superior genes, and advancement of marker-assisted breeding. Full article

Modern olive breeding points to a plant model characterized by low vigour, high productivity, and resistance to biotic and abiotic stresses, all traits required by the intensive and superhigh-density (SHD) systems of olive tree growing. The Italian Don Carlo and FS-17 Favolosa stand out among the new cultivars that are being tested. They were obtained not by breeding but by mass selection from two seedling populations of the Frantoio cultivar (maternal parent). Here, a multidisciplinary approach was used to determine the paternal parent of Don Carlo and FS-17, and then to investigate the inheritance of interesting traits such as fruit cell dimensions and oil content in these cultivars. Microsatellites were applied in phylogeny and kinship analyses, along with two functional markers previously developed on OeACP1 and OeACP2 genes. Ascolana Tenera cultivar was identified as the paternal parent of both new cultivars. This result was also supported by the analysis of the self-incompatibility group of the new cultivars and their most likely paternal parents. Light and electron microscopy [Cryo Scanning Electronic Microscopy (CRYO-SEM), Electronic Scanning Microscopy (E-SEM), and Transmission Electron Microscope (TEM)] techniques were used to analyze the fruit development concerning oil accumulation. Significant differences in cuticle thickness, size and shape of mesocarp and exocarp cells, and oil content were detected among cultivars. Our results suggested that the rearrangement of the traits studied led to an improved progeny compared to the parents. FS-17 exhibited an oil storage efficiency higher than Frantoio. Don Carlo showed fruit traits and oil content almost intermediate between the parents, making it a dual-purpose cultivar. Full article

Background/Objectives: After perinatal loss, namely stillbirth and neonatal death, many bereaved mothers continue to produce breast milk, facing the decision as to whether to suppress lactation or donate their milk. Our aims were to explore the experiences and views of Muslim mothers who had donated their breast milk following perinatal loss and examine the Islamic legal-ethical perspectives relating to milk donation. This research explores how milk donation serves as a coping mechanism and how Islamic teachings frame its permissibility and ethical considerations. Methods: A qualitative research methodology was employed, using a Interpretative Phenomenological Analysis (IPA). Nine bereaved Muslim mothers who had donated their breast milk and three Islamic religious scholars (an Imam, a Mufti, and a Muslim jurist) participated in in-depth interviews. Thematic analysis identified recurring patterns and insights. Results: Our findings revealed that mothers experienced milk donation as a coping mechanism, allowing them to maintain a symbolic connection with their lost child while contributing to other infants’ survival. Religious scholars who we interviewed agreed that milk donation is permissible in Islam, provided that milk kinship (rida’a) regulations are observed. Mothers reported a strong need for structured support from healthcare providers and religious leaders in order to assist in the informed decision-making process. Conclusions: Breast milk donation after perinatal loss aids in grief management for bereaved mothers while benefiting vulnerable infants. Healthcare providers should offer comprehensive lactation counseling for bereaved mothers, including milk donation options. Milk banks should implement processes in alignment with rida’a guidelines. Improving support systems for bereaved mothers can alleviate their grieving process while ensuring alignment with cultural and religious norms. Full article

Background/Objectives: The further application of high-coverage whole genome sequencing in fields such as paleogenomics, forensic investigations, and conservation genomics is impeded by two major barriers: extremely high costs and stringent sample requirements. Utilizing low-coverage sequencing offers a practical solution to these constraints; however, this approach introduces a primary challenge—the necessity to reconstruct distorted genomic information for downstream analysis. Methods: Analytical experiments conducted on low- to medium-coverage sequencing data confirmed the accuracy of several existing methods for inferring relationships up to the third degree and distinguishing unrelated individuals. Subsequently, efforts were made to evaluate allele-frequency-independent methods within animal genomics, where analyses are likely to encounter challenges such as uncertain allele frequencies, diverse sample types, and suboptimal sample quality. Kinship inference was performed on a total of 33 pairs of animal samples across three species, comprising nine parent–offspring pairs and four full-sibling pairs. Results: The analysis revealed that two efficient algorithm implementations (READ and KIN) successfully identified all unrelated pairs. Notably, among the various algorithms utilized, only KIN exhibited confusion between first- and second-degree relationships when subjected to. Conclusions: This study has filled a critical gap in the existing literature by conducting a comprehensive evaluation of various algorithms on low-coverage sequencing data derived from authentic human and animal samples, accompanied by detailed ground truth—a vital task that has been overlooked. Full article

Against the backdrop of global rural decline and China’s Rural Revitalization Strategy (2017–2050), this study examines the spatial form evolution and development mechanism of Central Village in Huotong Town, Ningde. To achieve this, it employs field surveys, spatial mapping, and hierarchical element analysis to analyze the village’s scale, architectural texture, street interfaces, and landscape nodes. Results show three evolutionary stages: pre-2017 traffic-guided disorderly expansion, 2017–2020 policy-driven orderly planning, and post-2020 stock optimization, forming an “oval radial” structure constrained by the southern hills and Huotong River. The spatial structure shifted from a “kinship-based agglomeration” to a multi-center model, yet contradictions like historical texture damage and excessive street commercialization emerged. Its development is driven by four linked factors: policy, the market, socio-culture issues, and nature. This study offers references for similar rural settlements’ spatial planning and sustainability. Full article

Hikayat Lonthoir, a rare saga manuscript collection originating from the Banda Archipelago, Maluku, Indonesia, retains significant Indigenous oral history amidst the Western colonial narrative. This study seeks to leverage computational methods to analyze the historic manuscript that constitutes a combination of OCR-supervised transcription, corpus linguistic profiling, semantic clustering (Word2Vec + K-Means), and named entity network analysis. A validation of the dataset is performed on 2793 cleaned word tokens towards Indonesian and Malay dictionaries, showing that 50.3% overlapped with both dictionaries, with strong cross-dictionary agreement (κ = 0.76). The lexical analysis indicates that monarchy/governance, kinship, maritime vocabulary, and extensive morphological productivity (me-, di-, ter-, pe-/per-, -nya, -an), while semantic and network analyses identify two narrative cores, developed into Aarne–Thompson–Uther (ATU) and Stith Thompson’s Motif Index of Folk Literature classification systems. These findings demonstrate how computational methods can extract structural, thematic, and relational patterns from historical manuscripts and contribute evidence-based insights to digital philology and historical linguistics. Full article

Duolang sheep, a meat–fat dual-purpose breed indigenous to Xinjiang, China, has been cultivated traditionally by the local Uyghur people for its prolificacy and precocious sexual maturity, while little research on the population structure and trait inheritance characteristics of Duolang sheep is available. This study employed whole-genome resequencing data from a cohort of 60 Duolang sheep to dissect their genetic population structure and genes related to reproductive traits. A total of 1565 Gb of high-quality data with an average depth of 14.06× was generated. After SNP calling and quality control, 31,300,060 SNPs were identified. Following linkage disequilibrium (LD)-based pruning, a total of 4,479,177 high-quality SNPs were retained for subsequent analyses. Based on these SNPs, the internal genetic structure of the Duolang sheep population was elucidated, with 14 kinship outliers detected through principal component analysis (PCA). Furthermore, LD decay analysis revealed that the r² declined below 0.1 at approximately 10 kb, indicating a relatively low level of selection pressure in the population. Within the population, Tajima’s D and iHS methods detected 517,218 and 82,534 candidate SNPs under selection, respectively, with 24,453 SNPs overlapping between the two methods. By splitting Duolang sheep into single-lamb (n = 29) and multiple-lamb (n = 12) subgroups according to litter size, 267,654 SNPs were identified by XP-CLR, while 184,179 SNPs suffering from selection were detected by F_ST and 62,150 by XP-EHH. Functional enrichment analysis of selected genes reveals the selection directions (domestication, growth, and reproduction) and related candidate genes in the Duolang sheep population, including ESRRA, ESRRB, OXT, FSHR, ESR2, GNRHR, and BMPR1B. This study provides the first comprehensive genomic landscape of Duolang sheep, elucidating genetic signatures of its adaptive traits. Full article

Genealogy is a powerful analytical framework for understanding the cultural evolution of tribes, communities, and societies. This article demonstrates that the recurrent reliance on genealogical structures is a common feature of human societies, serving as a fundamental mechanism for cultural evolution through time, space, and culture. Based on comparative analysis of indigenous tribal societies (e.g., Aboriginal Australian kinship, Polynesian chiefly genealogies), agrarian civilizations (e.g., European feudal lineages, Chinese patriliny), and modern nation-states (e.g., nationalist mythmaking, DNA-based ancestry movements), this study reveals consistent patterns in genealogical functions. Drawing on an interdisciplinary perspective from anthropology, sociology, history, and evolutionary biology, it is argued that genealogical systems are not passive records of descent but dynamic forces of cultural continuity and adaptation. The evidence shows that, despite vast sociocultural differences, genealogy widely operates as a dual-purpose instrument. It preserves cultural memory and legitimizes political authority while simultaneously facilitating social adaptation and innovation in response to new challenges. The paper also critiques contemporary trends like commercial genetic genealogy, highlighting its potential for reconnecting diasporic communities alongside its risks of biological essentialism. Ultimately, the work establishes that the persistent and patterned reliance on genealogy from oral traditions to genetic data offers a critical lens for understanding the deep structures of cultural continuity and transformation in human societies. It further underscores the importance of genealogy in cultural evolution, historical persistence, societal transformation, and the construction of belonging in an increasingly globalized world. Full article

During the COVID-19 pandemic, migrant workers in Singapore endured one of the longest and most stringent periods of confinement globally. Segregationist policies were intensified as the state imposed strict disciplinary regimes over workers’ mobility and everyday lives, framed as public health interventions but functioning also as labor discipline and social control. This study asks: how did migrant workers experience, narrate, and endure life under such conditions of confinement? Drawing on sixteen in-depth interviews with South Asian male construction workers, conducted in dormitories and makeshift worksites, we adopt a grounded theory approach to elicit contextually grounded accounts of life under lockdown. The analysis highlights three interrelated themes: emotional regulation, migrant masculinity and the gendered politics of endurance, and digital connectivity as an affective infrastructure. These practices enabled workers to carve out agentic spaces within structures designed to render them passive. Our findings reveal that even amid fear, surveillance, overcrowding, and economic precarity, workers combined stoicism, transnational kinship ties, religious routines, and solidarity to sustain resilience. While initially guided by Foucauldian notions of surveillance and biopower, the study advances a counter-Foucauldian insight: that institutional control is never total, and migrant narratives of resilience offer nuanced understandings of agency under constrain. Full article

Local livestock genetic resources are crucial for sustainable agriculture and biodiversity conservation. Dengchuan cattle, a nationally protected dairy breed in China, are esteemed for their high milk fat content and cultural significance. However, they have been threatened by crossbreeding with exotic high-yielding breeds, resulting in a decline in purebred resources. In this study, we evaluated the genetic diversity and structure of a conserved population using 100K SNP microarray data from 74 individuals. After implementing strict quality control measures, 78,460 loci were retained for principal component analysis (PCA), which identified 100 SNPs most associated with PC1. After calculating high-consistency loci using PLINK, based on allelic consistency, we selected 61 high-stability markers to represent 60 individuals for further analysis. Genetic diversity parameters indicated moderate polymorphism, with an effective population size (Ne) of 2.293, observed heterozygosity (Ho) of 0.300, expected heterozygosity (He) of 0.326, and an average polymorphic information content (PIC) of 0.261. A paired t-test confirmed a highly significant difference between Ho and He (p < 0.001). Runs of homozygosity (ROH) revealed a moderate level of inbreeding (F_ROH = 0.0928), with bulls exhibiting slightly higher values than females. Neighbor-joining (NJ) clustering further indicated clear lineage distinctions among bulls, but lower kinship among females. Overall, Dengchuan cattle exhibit moderate genetic diversity but face risks due to a small Ne and an unbalanced family structure. Targeted breeding strategies and genetic monitoring are recommended to ensure sustainable conservation and utilization. Full article

This article illuminates the cosmology of Raa Haqi (often called Dersim Alevism or Kurdish Alevism), a rarely examined strand within Alevi Studies. Existing scholarship’s emphasis on identity politics and sparse ethnography has left Raa Haqi’s mythological and cosmological dimensions underexplored. This paper approaches Raa Haqi through a dual authority framework: (1) Ocak lineages and Ocak–talip relations—sustained by kinship institutions like kirvelik, musahiplik, and communal rites such as the cem—and (2) jiares, non-human agents from the Batın realm that manifest in Zahir as sacred places, objects, and animals. Methodologically, I conduct a close, motif-based reading of folktales compiled by Caner Canerik (2019, Dersim Masalları I), treating them as ethnographic windows into living theology. The analysis shows that tales encode core principles—rızalık (mutual consent), ikrar (vow), sır (the secret knowledge), fasting and calendrical rites, ritual kinship, and moral economies involving humans, animals, and Batın beings. Dreams, metamorphosis, and jiare-centered orientations structure time–space, ethics, and authority beyond the Ocak, including in individual re-sacralizations of objects and sites. I conclude that these narratives do not merely reflect belief; they actively transmit, test, and renew Raa Haqi’s cosmological order, offering Alevi Studies a theory-grounded, source-proximate account of Kurdish Alevi mythic thought. Full article