Search Results (327)

Alfalfa (Medicago sativa L.) is widely recognized as a major forage crop, yet its role as a multifunctional biological input in sustainable horticultural production remains underexplored. This review evaluates alfalfa as a biological nitrogen source, organic fertilization resource, and biofertilizer-supporting crop within vegetable, medicinal, and perennial horticultural systems. Due to its high capacity for biological nitrogen fixation, alfalfa can supply substantial amounts of plant-available nitrogen, reducing dependency on synthetic fertilizers and supporting environmentally sound nutrient management. When used as green manure, cover crop, intercrop, mulch source, compost feedstock, or processed organic fertilizer, alfalfa enhances the soil organic carbon (SOC), improves soil structure, and increases the water-holding capacity properties particularly critical in intensive horticultural production. Higher SOC levels also contribute to the improved tolerance of horticultural crops to drought and heat stress through enhanced soil moisture retention and rhizosphere buffering. Alfalfa-based organic inputs stimulate rhizosphere microbial biomass, enzymatic activity, and functional genes associated with nitrogen cycling, strengthening plant–microbe interactions that underpin biofertilizer effectiveness. Evidence from vegetable and perennial systems indicates that alfalfa-derived amendments and rotations increase soil nitrogen availability, support yield stability, and improve soil health over the long-term. In orchards and vineyards, alfalfa cover cropping contributes to carbon sequestration, erosion control, and enhanced soil biological functioning. Overall, alfalfa emerges as a strategic species for integrating organic fertilization and biofertilizer-based approaches into modern horticultural systems, supporting reduced mineral fertilizer inputs while sustaining productivity, soil health, and environmental quality. Full article

To clarify the effects of biochar application on leaf photosynthesis, dry matter accumulation, and productivity in a maize–soybean intercropping system, a two-year field experiment was conducted in the Yellow River irrigation area of Inner Mongolia from 2024 to 2025. A split-plot design was adopted with two biochar application rates (0 and 5 t ha⁻¹) and three cropping patterns, including maize monoculture, soybean monoculture, and maize–soybean 2:4 intercropping. Leaf SPAD values, photosynthetic characteristics (Pn, Tr, Gs, and Ci), yield components, and land equivalent ratio (LER) were determined. Compared with maize monoculture, intercropping significantly increased maize SPAD values at the V12 and VT stages by 12.80% and 13.39% in 2024 and by 15.41% and 20.58% in 2025, respectively, and enhanced maize Pn, Tr, and Gs at the V12 and R1 stages. Soybean showed greater sensitivity to intercropping, with reduced SPAD values, Pn, Tr, and Gs during the branching, flowering, and pod-setting stages, whereas biochar application partially alleviated these inhibitory effects. Intercropping increased maize kernel number per ear and thousand-kernel weight but reduced soybean effective plant density, grain number per plant, and grain yield. Biochar application improved the grain yield of both intercropped maize and soybean. Under biochar application, the LER values reached 1.04 in 2024 and 1.21 in 2025, indicating a clear advantage in land-use efficiency. Overall, biochar application and maize–soybean intercropping were associated with improved photosynthetic performance, higher land-use efficiency, and increased system productivity. Full article

Intercropping systems are beneficial for resource utilization; however, the spatial proximity of companion species leads to competition for shared resources, particularly light. A walnut–potato intercropping model was established to understand the photosynthetic and physiological mechanisms underlying yield and marketability responses. Three intercropping treatments were established based on the number of potato ridges between walnut tree rows: B1 (three ridges), B2 (five ridges), and B3 (seven ridges). All intercropping and monoculture (CK) plots used an identical double-row planting pattern per ridge. Results showed that ridge density induced significant physiological changes and yield impacts. Compared to CK, B3 significantly reduced soluble protein content, net photosynthesis (Pn), and antioxidant enzyme activities (SOD, CAT), while B1 and B2 showed intermediate, non-significant reductions. Peroxidase (POD) activity increased progressively with ridge number (B3 > B2 > B1 > CK), indicating dose-dependent shade stress. Intercellular CO₂ concentration (Ci) was significantly elevated under all intercropping treatments, suggesting a predominantly non-stomatal, biochemical limitation on photosynthesis rather than water stress. Yield was highest in CK, followed by B1 and B2—which were statistically comparable to CK—while B3 yielded the least due to severe shading. Marketability declined sharply in B3, with fewer than half of tubers reaching commercial grade. Multivariate analysis showed distinct clustering of yield-associated variables (Pn, protein, marketability) separate from shade-stress indicators (POD, Ci) across treatments. These findings provide practical and scientific evidence to optimize walnut–potato intercropping configurations under the arid conditions. Full article

The study evaluated the effect of intercropping Paulownia (Paulownia spp.) with spring barley (Hordeum vulgare L., cv. KWS Thalis) on selected components of agroecosystem biodiversity under Polish conditions. A field experiment established in 2019 compared an alley cropping system with barley monoculture during the 2025 growing season. Weed infestation, soil microbial communities, mesofauna abundance, and crop yield were assessed. Weed abundance was lower in the intercropping system than in monoculture, reaching 5.6 vs. 15.6 plants m⁻² at BBCH 21 and 21 and 22.8 vs. 35.6 plants m⁻² at BBCH 75. Bacterial alpha diversity was significantly higher under intercropping conditions, with Shannon index values ranging from 5.12 to 5.25, compared with 4.98–5.09 in monoculture. Fungal diversity showed moderate differences between systems, whereas the abundance of Collembola and Acari was influenced mainly by seasonal variation rather than by cultivation system. No significant reduction in barley yield was observed under intercropping conditions. The results suggest that Paulownia-based alley cropping may reduce weed pressure and support selected soil biological properties without negatively affecting crop productivity. However, the observed responses varied depending on the analyzed parameter and sampling period, indicating the preliminary and context-dependent character of the results. Further long-term studies are required to better understand the ecological mechanisms operating in such agroforestry systems. Full article

Nitrogen (N) fertilization is a fundamental agronomic practice that governs crop productivity, yet its effects on the molecular composition and chemical stability of soil organic carbon (SOC) remain poorly understood, especially in cereal–legume intercropping systems. Traditional studies have focused on total SOC stocks rather than molecular-level changes, and the mechanistic pathway linking N addition to SOC functional group transformation remains unclear. This study addressed these critical gaps by investigating how graded N addition (0, 180, 270, and 360 kg N ha⁻¹) reshapes SOC chemistry in a subtropical soybean–maize intercropping system. Soil physicochemical properties, inorganic N pools, N-transformation enzyme activities (urease, nitrate reductase, and glutaminase), microbial biomass indices, labile organic carbon fractions (particulate, mineral-associated, and dissolved organic carbon), and SOC functional groups characterized by Fourier transform infrared (FTIR) spectroscopy were quantified across a two-year field experiment (2024–2025). Results showed that increasing N rates significantly elevated nitrate nitrogen (NO₃⁻-N) accumulation while depressing soil pH. Nitrogen-transformation enzymes, especially nitrate reductase and glutaminase, responded strongly and positively to the N gradient. Microbial biomass carbon (MBC) and nitrogen (MBN) increased with moderate N input but exhibited saturation or decline at 360 kg N ha⁻¹, accompanied by reduced microbial carbon use efficiency (CUE) and a lower MBC/MBN ratio. Among labile carbon fractions, dissolved organic carbon (DOC) was the most responsive pool, increasing markedly with N addition and correlating strongly with NO₃⁻-N. FTIR analysis revealed that N addition shifted SOC functional group composition toward chemically recalcitrant structures: the relative abundances of aromatic C=C and carbonyl C=O groups increased significantly, whereas labile C–O groups declined. Random forest modelling identified C=C, NO₃⁻-N, and DOC as the three most influential predictors of SOC chemical composition. Structural equation modelling (SEM) demonstrated a sequential mechanistic pathway: N fertilization increased NO₃⁻-N, which stimulated glutaminase activity and enhanced DOC, ultimately promoting C=C/C=O stabilization and explaining 91.3% of the variance in SOC aromaticity. These findings reveal that N addition does not merely augment SOC quantity but fundamentally transforms its molecular architecture toward greater chemical stability through a nitrate-mediated, enzyme–labile carbon coupling mechanism. This study provides a novel spectroscopic–mechanistic framework for understanding carbon–nitrogen interactions in intercropping agroecosystems and informs precision N management strategies aimed at simultaneous crop production and long-term soil carbon sequestration. Full article

Planting green manure between rows is an excellent green orchard cultivation practice. However, there is a lack of research on the application of such measures in vineyards. In this study, the ‘Beibinghong’ grape was used as experimental material, and clear tillage was used as a control. The effects of intercropping rape and pea between rows for two consecutive years on soil microecology and grape and wine quality were studied. The main results were as follows: intercropping green manure increased the berry pH. Intercropping green manure differentially modulated phenolic profiles in grape berries: pea intercropping significantly increased total phenolic and tannin contents relative to clean tillage across both years, whereas rapeseed intercropping showed variable effects depending on phenolic class and vintage. Green manure treatments also altered the accumulation of aldehydes, alcohols, and terpenoids. The intercropping of green manure could effectively reduce soil temperature and maintain soil moisture in the surface soil layer, reduce soil pH and electrical conductivity, and increase soil microbial biomass, aggregate amount, enzyme activity and soil fertility. Intercropping green manure changed soil microbial diversity and community structure. At the phylum level, the relative abundances of Chloroflexi (bacteria) and Mortierellomycota (fungi) were significantly increased. At the genus level, the genera Plectosphaerella and Alternaria—both dominant saprotrophic fungi—were also significantly enriched. The results of a comprehensive evaluation of principal component and membership function and sensory evaluation showed that intercropping peas was the best strategy. Soil pH, electrical conductivity, nitrate content, LAP activity, and the phyla Acidobacteriota, Chloroflexi, and Mortierellomycota were significantly correlated with the acid content of wine, while soil enzyme activity was significantly correlated with the phenolic content of wine. These results indicated that intercropping green manure could drive the quality of grapefruits and wine by regulating soil nutrients, enzyme activities, basic physical and chemical properties, and microbial communities. Full article

The early detection and spatial characterization of crop damage are critical for improving decision-making in precision agriculture, particularly in regions where traditional monitoring methods are limited in scalability and objectivity. This study presents an integrated information processing framework that couples UAV-based image acquisition, instance segmentation, slicing-aided inference of large orthomosaics, and georeferenced spatial analysis into a single reproducible pipeline for the detection and mapping of crop damage. The framework is applied to maize cultivated under traditional milpa systems in Yucatán, Mexico, a region characterized by intercropping, irregular plant spacing, and complex backgrounds rarely represented in mainstream agricultural deep learning benchmarks. High-resolution RGB images were systematically acquired over maize fields in Yucatán, Mexico, and curated into specialized datasets representing parcels, individual plants, and damaged vegetation. Instance segmentation models based on the YOLOv11 architecture were trained and evaluated to extract visual information related to crop condition, while the Slicing-Aided Hyper Inference (SAHI) method was integrated to enable efficient processing of large orthomosaic images. The proposed framework achieved high performance in detecting maize plants, with a precision of 92.9% and an mAP50 of 94.2%, and demonstrated reliable identification of damage patterns associated with Spodoptera frugiperda, reaching a precision of 79.2% and an mAP50 of 71.7%. The resulting georeferenced outputs provide spatially explicit information that supports quantitative analysis of crop health and damage distribution. The results indicate that the proposed framework constitutes a scalable and reproducible approach for UAV-based visual information extraction, with potential applicability to broader agricultural monitoring and data-driven decision support systems. Full article

Lentils constitute a strategically important crop within sustainable agricultural systems, particularly in the context of rising global demand for plant-based protein sources. In Switzerland, approximately 95% of lentil seeds are imported, underscoring the untapped potential for domestic production. This study systematically evaluated the performance of multiple lentil genotypes, alongside optimal seeding densities and growing seasons, through a series of field experiments conducted over five years. In addition, a sensory evaluation was performed on 12 selected genotypes to assess consumer-relevant quality traits. The findings revealed substantial variability in yield among genotypes, ranging from 0.9 to 1.6 t/ha; however, interannual variation exerted a more pronounced influence, with yields fluctuating between 0.1 and 2.0 t/ha. Notably, autumn-sown lentils achieved yields of up to 2.7 t/ha in three out of four growing seasons, even among genotypes lacking full winter-hardiness, indicating significant production potential under appropriate management conditions. Optimal plant densities were identified within the range of 180–240 plants/m². From an economic standpoint, higher seeding densities appear justifiable, as the increased seed costs are offset by corresponding gains in yield. Since intercropping of lentils with oats did not negatively affect grain yield nor the thousand kernel weight, the benefits of this cropping system are highlighted. Sensory analysis demonstrated statistically significant differences in attributes such as mealiness and juiciness, leading to the classification of genotypes into three distinct sensory clusters. Despite these differences, overall sensory variation was relatively limited, suggesting that genotype selection may be guided primarily by agronomic performance, climatic adaptability, and winter-hardiness, as well as by market preferences for seed colour and size. Collectively, these results highlight the potential of autumn sowing as a viable strategy to enhance lentil production and reduce the risk of crop failure in Swiss agricultural systems. Full article

Damage caused by Frankliniella intonsa to sunflower seeds results in the emergence of rusty speckling on the seedcoat, severely compromising seed quality in recent years. Although chemical control has remained the primary management strategy, its application during the flowering period—when F. intonsa is the most active—poses significant risks to pollinating insects and natural enemies, highlighting the urgent need for effective and environmentally sustainable control alternatives. Previous studies have shown that F. intonsa is attracted by buckwheat and that it could be a promising trap crop for F. intonsa. Thus, the attractiveness of Fagopyrum esculentum and F. tataricum to F. intonsa was compared, and the preference of F. intonsa between two buckwheat varieties was examined. Furthermore, the behavioral responses of F. intonsa to volatiles emitted by these plants in different developmental stages were assessed. The study results indicated that F. intonsa had a clear preference for F. tataricum over F. esculentum. In cage trials, the selection rates of 2nd instar nymphs and adults of F. intonsa for F. tataricum were 61.63% and 60.19% at the seedling stage, and 60.74% and 62.50% at the full-bloom stage, all significantly surpassing those of F. esculentum. Olfactory bioassays further confirmed that flowers of F. tataricum were notably more appealing to both 2nd instar nymphs and adults of F. intonsa, with selection rates of 64.17% and 61.67%, respectively. Twenty distinct floral volatiles of two buckwheat varieties were detected through the phytochemical analysis. Orthogonal partial least squares-discriminant analysis (OPLS-DA) identified seven key compounds that accounted for the observed behavioral differences. Both 2nd instar nymphs and adults of F. intonsa demonstrated a significant selection for Δ-Cadinene, with the highest selection rates of 75.00% and 76.67% recorded at a concentration of 0.1 μg/μL. Furthermore, F. intonsa exhibited a marked attraction to higher concentrations of Verbenone, which was unique to F. tataricum, and (S)-2-Methyl-1-butanol, which was unique to F. esculentum. Field intercropping experiments confirmed that F. tataricum outperformed F. esculentum in trapping F. intonsa within sunflower plots. In conclusion, the results indicated that F. tataricum possessed considerable potential as a trap crop for the integrated management of F. intonsa in sunflower cultivation systems. Full article

In the context of sustainable agriculture and the need to reduce mineral nitrogen inputs, intercropping cereals with legumes is increasingly considered a promising strategy to enhance nutrient use efficiency and improve feed quality. However, the effects of such systems, combined with varying nitrogen fertilization levels, on the dynamics of micronutrients in soil and plant biomass remain insufficiently explored. Field research was conducted in central Poland, in Ciechanów, from 2021 to 2023, during the months of April through July each year. The aim of the study was to analyze the impact of intercropping spring barley and spring triticale with narrowleaf lupin and varying mineral nitrogen fertilization (0–60 kg N ha⁻¹) on the concentration and uptake of Mn, Cu, Zn, and Fe in the soil and green matter intended for fodder. It was shown that both the sowing pattern and the level of N fertilization significantly differentiated the concentration of microelements in the soil and their concentration and uptake with the yield. As the proportion of lupine in the mixture increased, the post-harvest soil showed higher concentrations of Mn (2–8%), Cu (2–9%), Zn (9–33%), and Fe (4–10%), accompanied by a marked increase in their levels in green matter, ranging from 6% to 94% depending on the micronutrient. The highest uptake of micronutrients was obtained in intercropping systems with a predominance of legumes, especially with moderate fertilization (40–60 kg N ha⁻¹), where the growth ranged from 16% to as much as 139%. Compared to single-species crops, the intercropping system was characterized by higher efficiency of soil resource use and better mineral quality of the feed. The results indicate that the integration of legumes with cereals can be an effective tool for improving feed security while reducing the intensity of mineral fertilization, in line with the principles of sustainable agriculture. Full article

Although silvopastoral systems are widely recognized to improve ecosystem services, empirical evidence regarding their long-term effects on herbaceous diversity during succession remains scarce. In 2011, we established a silvopastoral experiment in the low hilly area of western Henan Province, China. Four pure forest plots—poplar (Populus simonii, PS), oriental thuja (Platycladus orientalis, PO), Chinese cork oak (Quercus variabilis, QV) and black locust (Robinia pseudoacacia, RP)—were planted on natural wasteland, with perennial alfalfa (Medicago sativa, MS) intercropped to form silvopastoral systems (PS-MS, PO-MS, QV-MS, RP-MS), with a natural wasteland plot serving as the control (CK). In July 2024, we investigated the species density, richness, and aboveground biomass of herbaceous communities across all plots. Species composition differed significantly between MS and CK, PS-MS and PS, and PO-MS and PO. Silvopastoral systems and MS generally exhibited higher density, richness, and biomass than pure forests and CK. Furthermore, MS and silvopastoral systems showed uniform density and biomass across slope positions, whereas CK and pure forests had higher values downslope, along with shifts in relative biomass. Except for MS and PS-MS, all plots had higher species richness downslope. Soil nutrient properties were closely correlated with the biomass, richness, density, and functional groups. These findings indicate that silvopastoral systems can significantly increase herbaceous density, richness, and biomass and alter species composition, with the effects varying by the dominant tree species. Full article

Intercropping systems can enhance land-use efficiency and generate early income in young orchards during the non-productive phase of perennial trees. This paper evaluates the agroeconomic performance of an organic blackcurrant–walnut intercropping system implemented under two planting configurations: (i) planting blackcurrant on walnut rows (782 plants/ha) and (ii) planting blackcurrant between walnut rows (1250 plants/ha). The study was conducted as a comparative case study in young walnut orchards in South-West Oltenia, Romania. Results indicate that, although the between-row configuration involves higher establishment and maintenance costs, it achieves higher yields and revenue, leading to improved economic efficiency and return on investment. These findings support intercropping as a viable strategy for enhancing the economic performance of young walnut plantations. Full article

Pea–cereal intercropping may combine ecological disease regulation with improved land-use efficiency, but field evidence for pea powdery mildew responses on the pea component under temperate continental conditions remains limited. A two-year field experiment (2017/2018 and 2018/2019) was conducted in Novi Sad, Serbia, to evaluate the effects of intercropping on pea powdery mildew disease index (DI%), pea grain yield, seed physical quality traits, and land-use efficiency. Winter pea cv. Kosmaj was grown as a sole crop or in mixed intercropping (70% pea + 30% cereal seeding rates) with wheat, triticale, rye, or oat in a randomized complete block design with four replicates. Powdery mildew DI% was assessed at BBCH 71–75, while pea grain yield, thousand-seed weight (TSW), hectoliter weight (HLW), and yield-based land equivalent ratio (LER) were determined at harvest. Under the low natural disease pressure recorded in the study, intercropping was associated with lower DI% than sole cropping (approximately 2.8-fold lower on seasonal means; p < 0.001), but DI% did not show a significant independent effect on pea grain yield, TSW, or HLW after accounting for year and cultivation system. Pea grain yield was generally lower under intercropping, although the magnitude of reduction depended on the cereal companion; pea–triticale maintained pea yield closest to the sole crop, whereas pea–oat showed the lowest pea yield. TSW and HLW were generally higher under intercropping, but additional analyses indicated that these traits reflected different response patterns. All intercrops achieved LER > 1, with the highest values recorded for pea–triticale. Full article

Pine plantations on volcanic slopes in Indonesia are considered to be forests and are managed for wood production and slope protection. Logging practices followed by replanting may affect soil health. Existing agroforestry management contracts allow farmers to intercrop with vegetables in young plantations and grow fodder grasses in older ones. However, critical data on hydrological functions in such systems are scarce, while concerns over heavy rainfall and floods increase. We explored the relationships between soil cover, soil carbon, earthworms, soil porosity and infiltration rates in relation to slope class in second-rotation pine plantations around two years of age (intercropped) and at ten-year old pine-grass stages. Five slope classes (0%–8%, 8%–15%, 15%–25%, 25%–45%, and >45%) were compared with three measurement points each. Basic soil chemical and physical characteristics were measured for the 0–10, 10–20 and 20–30 cm layers. Remnant natural forest was available as a historical reference only on the steepest slope class. Organic soil carbon (C_Org) divided by a texture-based reference level was 1.12, 0.32 and 0.49 for natural forest, young and old agroforestry on very steep slopes, respectively. Within pine-based agroforestry relative decline with slope class (1–5) was pronounced in earthworms (biomass −3.46, population −4.18) and infiltration rates (−2.35) while bulk density increased (0.49); for soil carbon (C_Org), nitrogen, available phosphorus and exchangeable Mg effects in the −1.26 to −1.68 range indicated a loss of functional topsoil. Differences with age of the agroforestry systems were much smaller but included a decreasing earthworm population but an increase in mean earthworm weight and partial recovery of the C_Org/C_Ref ratio. Pine-based agroforestry on very steep soils had only 10%–14% of the earthworm biomass and 35% of the infiltration rate of reference natural forest. Understory vegetation biomass and litter layer necromass were more than five-fold higher in the natural forest. Across all samples a higher C_Org and higher earthworm biomass were associated with complementary positive changes in infiltration rates and soil porosity. Regression analysis suggests equal skill of tree cover, soil C_Org, porosity, aggregate stability and earthworms to predict infiltration rates while explanatory variables were strongly correlated. Management of the pine plantations may have to achieve a closer approximation of the conditions in natural forests to effectively protect upper watersheds. Full article

A two-year field experiment was conducted in Tengchong, Yunnan, to evaluate the effects of tobacco monoculture (TM) and maize relay intercropping with tobacco (TIM) on subsequent tobacco growth and the rhizosphere microenvironment. Results showed that TIM significantly increased plant height by 11.8% and maximum leaf length by 12.4% at the vigorous growth stage without reducing yield. Although leaf chloride content increased and the potassium-to-chloride ratio decreased, both remained within high-quality ranges. Relay-cropped silage maize yielded 4.86 t·hm⁻², adding 1.70 × 10⁴ CNY·hm⁻². TIM reduced nitrogen accumulation in aboveground tobacco and temporarily lowered soil organic matter and available potassium, while increasing acid phosphatase, peroxidase, and urease activities. Soil bacterial α-diversity increased, with enrichment of beneficial genera, including Candidatus Solibacter, Talaromyces, and Penicillium. Metabolomics identified 1043 metabolites, with upregulation of galactinol, N-acetyl-L-tryptophan, and 3-dehydroshikimic acid, enriched in cyanogenic amino acid and cysteine–methionine pathways. PLS-PM and Mantel analyses indicated that relay-cropped maize indirectly regulates nutrient availability via microbial and metabolic pathways. These results show that maize relay intercropping creates a soil “legacy effect,” shifting the system from direct nutrient competition to microbially mediated nutrient buffering. Full article