Search Results (520)

The aim of this study was to assess whether edible insects reared on substrates containing food allergens can carry these allergens into the final product, and to evaluate the effectiveness of a pre-harvest fasting period in reducing this risk to provide consumer protection. Hermetia illucens larvae, chosen as the model species, were grown on substrates containing 10% of each of the following food allergens: peanut, almond, soy, celery, and gluten. At the end of the feeding period, larvae were sampled at T0 (end of feeding), T1 (24 h fasting), T2 (48 h fasting) and tested by real-time PCR and ELISA to detect allergen residues. Positive results were observed by real-time PCR for soy (mean Ct: 28.84 at T0, 29.4 at T1, 30.95 at T2), celery (mean Ct: 26.74 at T0, 26.90 at T1, 29.77 at T2) and almond (Ct 33.96 at T0 and mean Ct: 34.01 at T1). Soy presence was also confirmed by ELISA test. Insects may represent an alternative food source; however, their use requires careful evaluation due to the potential presence of allergens. Our results showed that insects may contain allergens originating from their feeding substrates, potentially triggering a response in allergic consumers. Full article

Increased demands for protein have led to a search for alternatives to traditional protein sources like soy and animal protein. Black Soldier Fly Larvae can be reared on many feedstocks to produce a high-quality nutrient source for livestock and pets. These insects contain biologically meaningful compounds like antimicrobial peptides, lauric acid, and chitin. This combination of compounds highlights the need to investigate BSFL as a functional ingredient in broilers. This study examined the impact of BSFL inclusion on broiler performance with and without a subclinical Necrotic Enteritis (NE) challenge over two experiments. In both experiments, diets included 0%, 2.5%, or 5.0% BSFL from 0–42 d. During Experiment 2, birds were given a live coccidiosis vaccine at hatch and challenged with C. perfringens at 19, 20, and 21 d. Primary variables include growth performance, lesion scores, and NE-specific mortality. A BSFL inclusion of 2.5% and 5.0% significantly improved feed conversion ratio in both experiments (p < 0.001); 5% BSFL inclusion also significantly improved body weight gain in both experiments (p = 0.014, p = 0.023, respectively). Overall, results indicate that BSFL is an effective and safe feed ingredient option for commercial broiler production, providing biologically relevant improvements in performance with and without disease pressure. Full article

Insect bioconversion can transform agri-food side-streams into insect biomass while returning nutrients in the form of frass. This study evaluated Tenebrio molitor reared on wheat bran and on substrates in which bran was partially replaced with agri-food side-streams produced in Kosovo, including brewer’s spent grain, brewer's spent yeast, apple and grape pomace, and surplus vegetables. Growth performance, larval composition, and frass nutrient composition were assessed across seven treatments. Larvae reared on wheat bran combined with brewer’s spent grain and melon as wet feed achieved the highest larval weight. Substrates containing wheat bran, apple pomace, and brewer spent yeast also supported high larval weights, but with a higher feed conversion ratio. In contrast, larvae reared on wheat bran, grape pomace, and brewer's spent yeast showed the lowest larval weights. The use of potatoes as wet feed was associated with a longer development period. Larval proximate composition remained similar across treatments, with crude protein contents of 52–56% DM and only limited variation in fat and ash content. Overall, several tested side-streams supported larval growth comparable to wheat bran while generating nutrient-containing frass. Full article

Insect farming generates frass as a co-product alongside insect biomass, creating interest in its valorization within circular bioeconomy strategies and in its use as a fertilizer, soil improver, or plant biostimulant. This review adopts a claim-led framework linking product classification, composition, post-treatment, microbiological safety, environmental risks, and the evidence required to support specific agronomic claims, with particular emphasis on the EU regulatory context. Evidence from incubation, pot, greenhouse, and field studies, together with regulatory and technical sources, show that frass is a heterogeneous material whose performance depends on insect species, rearing substrate, product fraction, soil conditions, application rate, and processing history. Its relevance is increasing, particularly in regions where insect farming is expanding under established regulatory and industrial frameworks, including the European Union, North America, and parts of Asia. Across the reviewed evidence, the most scientifically and regulatorily defensible current positioning of frass is as a product-specific fertilizer or soil improver, whereas broader biostimulant or plant-protection claims require stronger product-level evidence. The review further concludes that safe and credible deployment depends on transparent characterization, appropriate hygienization and storage, contaminant screening where relevant, and claim-specific alignment with the applicable regulatory route. Full article

Insect populations are increasingly exposed to concurrent climate warming and agrochemical contamination, yet how these stressors interact to influence reproductive performance remains poorly understood. Because fertility can constrain population growth before survival declines, understanding how environmental stress affects reproduction is essential for predicting demographic responses. Here, we investigated how elevated temperatures and sublethal imidacloprid exposure during development and early-life interact with the insecticide resistance locus Cyp6g1 to influence male reproductive performance in Drosophila melanogaster. Males were reared from embryo to adulthood under factorial combinations of temperature and insecticide exposure, and mating behaviour and fertilisation success were subsequently quantified under benign assay conditions. Early-life heat reduced fertilisation success in a genotype-dependent manner, with a pronounced collapse observed in insecticide-susceptible males. Sublethal insecticide exposure modified this thermal response, restoring fertilisation success in susceptible males and producing non-additive interactions between thermal and agrochemical stress. In contrast, although mating frequency varied across treatments, it did not show the pronounced decline observed in fertilisation success, indicating that behavioural engagement does not necessarily predict functional reproductive output. These results suggest that environmental stress experienced during early-life can reshape reproductive performance, potentially through genotype-dependent shifts in physiological investment. Considering developmental stress history and genetic variation will therefore be important for predicting insect population responses to climate warming and environmental contamination. Full article

The rapid expansion of edible insect production has focused primarily on rearing, processing efficiency, safety, and nutritional composition, while the slaughter of insects has received comparatively little scientific and ethical scrutiny. This narrative review examines insect slaughter as a critical control point linking bioethics, physiology, and ingredient quality. The review synthesizes evidence from neurobiology, food science, and processing studies to evaluate how commonly used slaughter methods interact with biological aspects of insects. Existing literature shows that slaughter techniques influence protein stability and hydrolysis, lipid oxidation, antioxidant retention, techno-functional properties such as emulsification and gelation, as well as sensory attributes and consumer acceptance. Available evidence suggests that methods designed to rapidly suppress metabolic activity may be associated with improved preservation of certain nutritional and functional parameters, although findings remain species- and context-dependent. The review further highlights major knowledge gaps, including the lack of species- and life-stage-specific welfare indicators and standardized assessment protocols. Overall, the findings support the need to reconceptualize insect slaughter as a strategic upstream decision rather than a neutral processing step. Integrating ethical considerations with nutritional, functional, and regulatory perspectives is essential for establishing science-based standards and ensuring the responsible development of edible insect-based food and feed systems. Full article

In China, the substantial gap between domestic soybean supply and growing consumption necessitates large-scale soybean imports. The use of cultivated soybean (Glycine max) leaves as feed for the edible insect Clanis bilineata tsingtauica reduces crop yield, posing a threat to national soybean production security. To address this issue, this study evaluated wild soybean (Glycine soja) as a potential alternative feed source. Comparative analyses examined the nutritional and anti-nutritional properties of G. max (cv. Qihuang34) and a laboratory-preserved G. soja germplasm, together with their effects on larval growth performance, nutritional composition, and associated microbiota. G. soja leaves exhibited significantly higher crude fat (5.61% vs. 2.17%), ash (11.07% vs. 9.62%), neutral detergent fiber (23.75% vs. 21.00%), calcium (4.05 g/kg vs. 3.41 g/kg), and phosphorus (2.52 g/kg vs. 2.38 g/kg) than G. max leaves, along with lower trypsin inhibitor levels (p < 0.01) despite higher phytic acid content (p < 0.05). Fifth-instar larvae reared on G. soja leaves showed a 12.9% greater body weight (6.846 g vs. 6.066 g), higher crude protein (672.14 g/kg vs. 555.02 g/kg), total soluble sugar (21.27 mg/g vs. 8.96 mg/g), and soluble protein (26.35 mg/g vs. 24.71 mg/g), but lower crude fat (187.44 g/kg vs. 205.82 g/kg, p < 0.05). 16S rRNA sequencing revealed distinct phyllosphere microbial communities, with G. soja enriched in diverse taxa (e.g., Bacteroidota, Proteobacteria) and G. max dominated by Firmicutes. Corresponding differences were observed in larval gut microbiota, and positive correlations suggested potential microbial transfer from G. soja leaves to larval guts. Overall, G. soja represents a promising alternative feed source for C. bilineata, reducing competition with soybean grain production and supporting sustainable insect farming. Full article

Food waste poses significant environmental, economic and public health challenges. The black soldier fly (Hermetia illucens) larvae (BSFL) represent a promising solution for organic waste valorisation, converting substrates into protein-rich biomass for animal feed and organic fertiliser. However, the use of food waste as an insect substrate remains prohibited in the European Union due to regulatory and safety concerns. This study evaluated the suitability of heterogeneous food waste for BSFL rearing under industrial conditions by comparing larval performance on a standard Gainesville diet (control) and a blend derived from local restaurant waste (test). The 14-day bioconversion assay assessed bioconversion rate (BCR), feed conversion ratio (FCR), survival rate, average growth rate, and nutritional composition. Compared with the control, the test group showed significantly improved (p < 0.001) BCR (18.34% vs. 11.02%), FCR (5.48 vs. 9.09 kg/kg), survival (69.29% vs. 51.30%), and growth (8.38 vs. 6.59 mg/day). Larvae reared on food waste also exhibited significantly higher protein (19.70% vs. 16.80%), fat (13.70% vs. 7.20%), ash (6.97% vs. 3.51%), carbohydrates (7.00% vs. 3.60%), and fibre (5.20% vs. 2.90%). Overall, heterogeneous food waste is a suitable substrate for BSFL, supporting agrifood sustainability; however, future research should focus on standardisation of these substrates. Full article

The escalating demand for sustainable, nutrient-dense feeds underscores the need to valorize the agro-industrial byproducts utilizing innovative bioconversion strategies. In this context, we have studied the feasibility of incorporating tomato (Solanum lycopersicum) cultivation residues into Black Soldier Fly (BSF) larvae diets to produce high-protein insect meals. These residues are known to contain the naturally occurring toxic steroidal alkaloids tomatidine and α-tomatine, prohibiting their incorporation into human and animal diets. Herein, the tomato cultivation biomass was dried and mill-ground, and its varying volumes were incorporated into standard poultry feed (seven diet levels with 0–100% biomass inclusion) and tested in BSF-larvae-rearing trials to produce insect meals. The optimal results with respect to larvae growth, protein accumulation (highest value = 30.61%), lipid–fiber content, and antioxidant capacity were determined for insect meals obtained from BSF larvae reared with a ration composed of 40% tomato plant biomass. In addition, the toxicity of this insect meal was substantially low, as a consequence of the observed groundbreaking reduction in the contained toxic steroidal alkaloids α-tomatine and its aglycone tomatidine. The results herein reveal the efficacy of the BSF-larvae-rearing process in acting as a biological filter for the bioconversion of the toxic tomato cultivation waste into a functional, safe, and protein-rich livestock feed, supporting the principles of a circular economy. Full article

Sustainable expansion of global aquaculture relies on innovative alternative diets that reduce dependence on marine-derived ingredients. Poultry by-product meal (PBM) and insect meal have emerged as promising protein sources, yet their combined use under commercial farming conditions remains poorly explored. This study evaluated a plant-based finishing diet low in marine proteins and supplemented with 10% Hermetia illucens larvae meal (HIM) and 30% PBM (H10P30) and compared it with a conventional commercial diet (COM) in gilthead sea bream (Sparus aurata) reared on a land-based farm for 65 days. Health and welfare indicators, product safety, fillet quality, fatty acid profile, oxidative status, and consumer acceptance were assessed. Fish fed the H10P30 diet showed a significantly higher body weight and specific growth rate and a lowered feed conversion ratio than COM-fed fish. No external or internal lesions or liver histopathological alterations related to the H10P30 diet were observed. While the diet influenced the fatty acid profile of raw fillets, differences disappeared after cooking, except for a higher C22:6n-3 content in cooked H10P30 fillets. Sensory analysis penalised COM fillets due to the perceived hard texture and low juiciness. In summary, incorporating both PBM and HIM into a plant-based finishing diet serves as a viable feeding strategy for gilthead sea bream, contributing to improved feed sustainability. Full article

The black soldier fly, Hermetia illucens (Linnaeus, 1758), is a globally recognized resource insect for waste bioconversion and sustainable resource provision. Understanding its larval sensory system is key to explaining feeding and environmental perception behaviors. This study used scanning and transmission electron microscopy to analyze the types, distribution, ultrastructure, and developmental consistency of sensilla on larval cephalic appendages. Five sensilla types were found on antennae, sensilla basiconica I–II, sensilla twig basiconica I–II, and sensilla campaniformia, each type comprising 2–6 sensilla. Mouthparts harbored ten types, including sensilla twig basiconica III–V, sensilla placodea, sensilla ligulate, sensilla digitiformia, sensilla trichodea, and sensilla chaetica I–III, with 2–9 sensilla per type. All sensilla showed constant numbers and positions throughout larval development. Ten sensillum types with cuticle pores were innervated by 2–6 sensory neurons, primarily suggesting chemoreceptors; these were concentrated at the tips of antennae and maxillary palps. Sensilla digitiformia on the palps possessed a non-porous cuticle and a single sensory cell, indicative of a thermo-/hygroreceptive role. Sensilla chaetica and trichodea, with non-porous cuticles and no dendrites, were mechanosensory. These results reveal the morphofunctional basis of larval sensation, supporting chemoreception studies and optimizing rearing via behavioral modulation. Full article

The bollworm/corn earworm, Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae), is one of the most economically damaging crop pests in North America. Colonies of H. zea are notoriously difficult to maintain and frequently collapse in laboratory rearing. The persistent difficulty in maintaining healthy H. zea colonies has become a major obstacle to performing many research activities on the insect. To optimize colony maintenance, six populations were evaluated across three trials and six tests examining pupal maturity at diet removal, adult emergence synchrony, and cohort size at mating and reproduction. Females emerging from mature pupae produced more eggs than those from mid-aged (5–7 d) or young pupae (0–2 d). Synchronizing male and female emergence within one day yielded higher mating frequency, spermatophore transfer, and progeny, whereas a two-day difference reduced these metrics by 45–67%. Adult cohort size also influenced the outcomes, with ≥10 males and ≥10 females per cage enhancing reproductive success. Most matings occurred on nights 2–3, peaking within 2.5 h after lights off. Positive correlations were observed among mating frequency, spermatophore transfer, and progeny production. Overall, optimal performance was achieved by removing pupae only at maturity, synchronizing adult emergence within one day, and maintaining larger adult cohorts. These findings should establish key conditions to improve the mating success, reproduction, and laboratory rearing of H. zea. Full article

Biological control using parasitoid wasps and the sterile insect technique (SIT) are environmentally sustainable strategies that can be integrated into fruit fly management programs. Both eco-friendly techniques have been applied independently against the invasive pest Ceratitis capitata (Wiedemann), commonly known as the Mediterranean fruit fly or medfly, in irrigated fruit-growing areas of San Juan Province, central-western Argentina. At the San Juan Biofactory, both sterile medfly males and the exotic larval parasitoid Diachasmimorpha longicaudata (Ashmead) are mass-reared using the Vienna-8 temperature-sensitive lethal (tsl) genetic sexing strain. The aim of this study was to assess the effectiveness of controlling the medfly by combining releases of D. longicaudata and sterile male flies under field cage (semi-field) conditions. Trials were conducted during the summer, from 31 January to 26 April 2019, at a fruit farm in the Rawson district of San Juan. Each mesh-covered cylindrical iron field cage enclosed five exposure devices, each holding three semi-ripe figs used as oviposition substrates. The experimental treatments were as follows: (1) control (no parasitoid or sterile fly releases), (2) parasitoid release alone (fertile flies from a biparental medfly strain which were released first, followed by parasitoids), (3) sterile medfly release alone (fertile flies and sterile males released simultaneously), and (4) combined techniques (fertile and sterile medflies released first, followed by parasitoids). The resulting dataset includes the number of recovered puparia and non-hatching puparia, adult flies and parasitoids, as well as the benefit proportion and Abbott’s effectiveness for each experimental condition. Combining both methods produced an additive suppression of the pest population, achieving 96% suppression of the medfly population, a value close to a near-eradication effect. These results support the use of both control techniques in an area-wide integrated medfly management approach. Full article

Insect farming for several purposes, which inscribes itself into circular economy, could become an alternative to traditional agriculture in Europe. Insects are a more sustainable and circular alternative source of protein and fat in food and feeds. The aim of this study is to identify legal barriers to the rearing of insects and marketing of insect-based products. The study focuses on the identification of such barriers to insect rearing and to the production of fertilizers from insect frass. The dogmatic legal method, as well as SWOT and PESTEL analyses, are employed in this research. The two latter methods are used to gain insight into the views held by the industry’s stakeholders. Subsequently, issues within the research field, such as the rearing of insects, their welfare, and the requirements imposed on the feeding of farmed insects, are discussed. Finally, solutions to the identified problems are suggested. The most important strengths of insect farming are its innovative edge and the creation of new products at the EU level. Weaknesses include technological and organizational challenges. Stakeholders attribute high importance to external circumstances, especially economic and social ones. As concluded from this study, the current laws are not optimal for insect farming; however, despite this situation, some changes to the law could facilitate the acquisition of feed for insects or the marketing of some insect-based products. The proposed legal changes aim at lifting the identified barriers to insect farming while still meeting safety requirements and supporting circular economy principles. Full article

Chagas disease, caused by Trypanosoma cruzi, is maintained in nature by complex interactions among wild vertebrates and triatomine insect vectors, yet the role of many introduced hosts remains poorly resolved. Here, we assessed natural T. cruzi infection in wild European rabbits (Oryctolagus cuniculus) from central Chile, where introduced rabbits overlap ecologically with the sylvatic vector Mepraia spinolai. Eight free-ranging rabbits captured in Las Chinchillas National Reserve were evaluated using an integrative diagnostic approach combining xenodiagnosis with laboratory-reared, parasite-free M. spinolai nymphs, real-time polymerase chain reaction targeting T. cruzi satellite DNA in blood and 12–14 organs per animal, and histopathology with immunohistochemistry (anti-cruzipain) to identify tissue parasite forms. Blood molecular detection was positive in seven out of eight rabbits, while xenodiagnosis detected viable parasites in two out of seven evaluated individuals. Organ molecular screening detected T. cruzi DNA in at least one organ in all rabbits, with frequent positivity in the diaphragm, reproductive tissues, spleen, and kidney. Histopathology identified parasite forms in four out of eight animals, and immunohistochemistry confirmed hepatic amastigotes in one case. These findings provide multi-method evidence of natural infection in the sampled individuals, including evidence of parasite viability in some individuals, suggesting potential epidemiological relevance within this ecological context and possible utility for surveillance in Chilean sylvatic transmission settings. Full article