Search Results (337)

Voltage-gated Ca²⁺ channels (VGCCs) are regulated by four CaVβ subunits (CaVβ1–CaVβ4), each showing specific expression patterns in excitable cells. While primarily known for regulating VGCC function, CaVβ proteins also have channel-independent roles, including gene expression modulation. Among these, CaVβ1 is expressed in skeletal muscle as multiple isoforms. The adult isoform, CaVβ1D, localizes at the triad and modulates CaV1 activity during Excitation–Contraction Coupling (ECC). In this study, we investigated the lesser-known embryonic/perinatal CaVβ1 isoforms and their roles in neuromuscular junction (NMJ) formation, maturation, and maintenance. We found that CaVβ1 isoform expression is developmentally regulated through differential promoter activation. Specifically, CaVβ1A is expressed in embryonic muscle and reactivated in denervated adult muscle, alongside the known CaVβ1E isoform. Nerve injury in adult muscle triggers a shift in promoter usage, resulting in re-expression of embryonic/perinatal Cacnb1A and Cacnb1E transcripts. Functional analyses using aneural agrin-induced AChR clustering on primary myotubes demonstrated that these isoforms contribute to NMJ formation. Additionally, their expression during early post-natal development is essential for NMJ maturation and long-term maintenance. These findings reveal previously unrecognized roles of CaVβ1 isoforms beyond VGCC regulation, highlighting their significance in neuromuscular system development and homeostasis. Full article

Background: BDE-47, a pervasive environmental pollutant detected in >90% of human serum samples, is increasingly linked to metabolic disorders. This study investigates the specific impact of BDE-47 exposure on the gut microbiota in prediabetic mice and evaluates the efficacy of therapeutic interventions in mitigating these effects. Objectives: To determine whether BDE-47 exposure induces diabetogenic dysbiosis in prediabetic mice and to assess whether dietary interventions, such as grape exosomes and an antioxidant cocktail, can restore a healthy microbiota composition and mitigate diabetes risk. Methods: In this study, a prediabetic mouse model was established in 54 male SPF-grade C57BL/6J mice through a combination of high-sugar and high-fat diet feeding with streptozotocin injection. Oral glucose tolerance tests (OGTT) were conducted on day 7 and day 21 post-modeling to assess the establishment of the model. The criteria for successful model induction were defined as fasting blood glucose levels below 7.8 mmol/L and 2 h postprandial glucose levels between 7.8 and 11.1 mmol/L. Following confirmation of model success, a 3 × 3 factorial design was applied to allocate the experimental animals into groups based on two independent factors: BDE-47 exposure and exosome intervention. The BDE-47 exposure factor consisted of three dose levels—none, high-dose, and medium-dose—while the exosome intervention factor included three modalities—none, Antioxidant Nutrients Intervention, and Grape Exosomes Intervention. Fresh fecal samples were collected from mice two days prior to sacrifice. Cecal contents and segments of the small intestine were collected and transferred into 1.5 mL cryotubes. All sequences were clustered into operational taxonomic units (OTUs) based on defined similarity thresholds. To compare means across multiple groups, a two-way analysis of variance (ANOVA) was employed. The significance level was predefined at α = 0.05, and p-values < 0.05 were considered statistically significant. Bar charts and line graphs were generated using GraphPad Prism version 9.0 software, while statistical analyses were performed using SPSS version 20.0 software. Results: The results of 16S rDNA sequencing analysis of the microbiome showed that there was no difference in the α diversity of the intestinal microbiota in each group of mice (p > 0.05), but there was a difference in the Beta diversity (p < 0.05). At the gate level, the abundances of Proteobacteria, Campylobacterota, Desulfobacterota, and Fusobacteriota in the medium-dose BDE-7 group were higher than those in the model control group (p < 0.05). The abundance of Patellar bacteria was lower than that of the model control group (p < 0.05). The abundances of Proteobacteria and Campylobacterota in the high-dose BDE-7 group were higher than those in the model control group (p < 0.05). The abundance of Planctomycetota and Patescibacteria was lower than that of the model control group (p < 0.05), while the abundance of Campylobacterota in the grape exosome group was higher than that of the model control group (p < 0.05). The abundance of Patescibacteria was lower than that of the model control group (p < 0.05), while the abundance of Firmicutes and Fusobacteriota in the antioxidant nutrient group was higher than that of the model control group (p < 0.05). However, the abundance of Verrucomicrobiota and Patescibacteria was lower than that of the model control group (p < 0.05). At the genus level, the abundances of Bacteroides and unclassified Lachnospiraceae in the high-dose BDE-7 group were higher than those in the model control group (p < 0.05). The abundance of Lachnospiraceae NK4A136_group and Lactobacillus was lower than that of the model control group (p < 0.05). The abundance of Veillonella and Helicobacter in the medium-dose BDE-7 group was higher than that in the model control group (p < 0.05), while the abundance of Lactobacillus was lower (p < 0.05). The abundance of genera such as Lentilactobacillus and Faecalibacterium in the grape exosome group was higher than that in the model control group (p < 0.05). The abundance of Alloprevotella and Bacteroides was lower than that of the model control group (p < 0.05). In the antioxidant nutrient group, the abundance of Lachnospiraceae and Hydrogenophaga was higher than that in the model control group (p < 0.05). However, the abundance of Akkermansia and Coriobacteriaceae UCG-002 was significantly lower than that of the model control group (p < 0.05). Conclusions: BDE-47 induces diabetogenic dysbiosis in prediabetic mice, which is reversible by dietary interventions. These findings suggest that microbiota-targeted strategies may effectively mitigate the diabetes risk associated with environmental pollutant exposure. Future studies should further explore the mechanisms underlying these microbiota changes and the long-term health benefits of such interventions. Full article

Leaf chlorophyll content (LCC), leaf area index (LAI), and above-ground biomass (AGB) are important growth parameters for characterizing potato growth and predicting yield. While deep learning has demonstrated remarkable advancements in estimating crop growth parameters, the limited availability of field data often compromises model accuracy and generalizability, impeding large-scale regional applications. This study proposes a novel deep learning model that integrates multi-task learning and few-shot learning to address the challenge of low data in growth parameter prediction. Two multi-task learning architectures, MTL-DCNN and MTL-MMOE, were designed based on deep convolutional neural networks (DCNNs) and multi-gate mixture-of-experts (MMOE) for the simultaneous estimation of LCC, LAI, and AGB from Sentinel-2 imagery. Building on this, a few-shot learning framework for growth prediction (FSLGP) was developed by integrating simulated spectral generation, model-agnostic meta-learning (MAML), and meta-transfer learning strategies, enabling accurate prediction of multiple growth parameters under limited data availability. The results demonstrated that the incorporation of calibrated simulated spectral data significantly improved the estimation accuracy of LCC, LAI, and AGB (R² = 0.62~0.73). Under scenarios with limited field measurement data, the multi-task deep learning model based on few-shot learning outperformed traditional mixed inversion methods in predicting potato growth parameters (R² = 0.69~0.73; rRMSE = 16.68%~28.13%). Among the two architectures, the MTL-MMOE model exhibited superior stability and robustness in multi-task learning. Independent spatiotemporal validation further confirmed the potential of MTL-MMOE in estimating LAI and AGB across different years and locations (R² = 0.37~0.52). These results collectively demonstrated that the proposed FSLGP framework could achieve reliable estimation of crop growth parameters using only a very limited number of in-field samples (approximately 80 samples). This study can provide a valuable technical reference for monitoring and predicting growth parameters in other crops. Full article

Background: Multiple Chemical Sensitivity (MCS) is a complex, disabling condition marked by non-specific symptoms in response to low-level chemical exposures. It often leads to substantial impairments in quality of life, psychological health, and daily functioning. Although non-pharmacological approaches—such as lifestyle and psychological interventions—are widely used, their clinical effectiveness remains unclear. Objective: We aim to evaluate the effectiveness of lifestyle-based approaches in improving clinical and psychosocial outcomes in adults with Multiple Chemical Sensitivity. Methods: A systematic review was conducted in accordance with PRISMA guidelines (PROSPERO: CRD420251013537). Literature searches were carried out in MEDLINE (PubMed), CINAHL, Google Scholar, and ResearchGate between March and April 2025. Eligible studies included adults (≥18 years) with a confirmed diagnosis of MCS and reported outcomes such as perceived stress, anxiety, depressive symptoms, or quality of life. Methodological quality and risk of bias were independently assessed using the PEDro scale, NIH Quality Assessment Tool, CEBMa checklist, and Cochrane RoB 2.0. Results: Twelve studies (N = 378) met the inclusion criteria. Cognitive and behavioral therapies demonstrated the most consistent evidence of efficacy, with reductions in symptom severity, maladaptive cognitive patterns, and functional limitations. Mindfulness-based stress reduction showed favorable outcomes, while other mindfulness-based interventions yielded mixed results. Exposure-based therapies contributed to increased chemical tolerance and reduced avoidance behavior. Electromagnetic and biomedical approaches demonstrated preliminary but limited effectiveness. Aromatherapy was well tolerated and perceived as relaxing, though its clinical impact was modest. Conclusions: Cognitive and behavioral therapies appear to be most effective among lifestyle-based interventions for MCS/IEI. However, study heterogeneity limits the generalizability of findings, underscoring the need for more rigorous research. Full article

In response to the challenges associated with weld treatment during the on-site corrosion protection of hydraulic steel gates, this paper proposes a method utilizing a magnetic adsorption climbing robot to perform corrosion protection operations. Firstly, a magnetic adsorption climbing robot with a multi-wheel independent drive configuration is proposed as a mobile platform. The robot body consists of six joint modules, with the two middle joints featuring adjustable suspension. The joints are connected in series via an EtherCAT bus communication system. Secondly, the kinematic model of the climbing robot is analyzed and a PID trajectory tracking control method is designed, based on the kinematic model and trajectory deviation information collected by the vision system. Subsequently, the proposed kinematic model and trajectory tracking control method are validated through Python3 simulation and actual operation tests on a curved trajectory, demonstrating the rationality of the designed PID controller and control parameters. Finally, an intelligent software system for weld defect detection based on computer vision is developed. This system is demonstrated to conduct defect detection on images of the current weld position using a trained model. Full article

Introduction: Huntington’s disease (HD) disrupts cortico-striato-thalamocortical circuits decades before clinical onset. Electroencephalography (EEG) offers millisecond temporal resolution, low cost, and broad accessibility, yet its mechanistic and biomarker potential in HD remains underexplored. We conducted a mechanistic review to synthesize half a century of EEG findings, identify reproducible electrophysiological signatures, and outline translational next steps. Methods: Two independent reviewers searched PubMed, Scopus, Google Scholar, ResearchGate, and the Cochrane Library (January 1970–April 2025) using the terms “EEG” OR “electroencephalography” AND “Huntington’s disease”. Clinical trials published in English that reported raw EEG (not ERP-only) in human HD gene carriers were eligible. Abstract/title screening, full-text appraisal, and cross-reference mining yielded 22 studies (~700 HD recordings, ~600 controls). We extracted sample characteristics, acquisition protocols, spectral/connectivity metrics, and neuroclinical correlations. Results: Across diverse platforms, a consistent spectral trajectory emerged: (i) presymptomatic carriers show a focal 7–9 Hz (low-alpha) power loss that scales with CAG repeat length; (ii) early-manifest patients exhibit widespread alpha attenuation, delta–theta excess, and a flattened anterior-posterior gradient; (iii) advanced disease is characterized by global slow-wave dominance and low-voltage tracings. Source-resolved studies reveal early alpha hypocoherence and progressive delta/high-beta hypersynchrony, microstate shifts (A/B ↑, C/D ↓), and rising omega complexity. These electrophysiological changes correlate with motor burden, cognitive slowing, sleep fragmentation, and neurovascular uncoupling, and achieve 80–90% diagnostic accuracy in shallow machine-learning pipelines. Conclusions: EEG offers a coherent, stage-sensitive window on HD pathophysiology—from early thalamocortical disinhibition to late network fragmentation—and fulfills key biomarker criteria. Translation now depends on large, longitudinal, multi-center cohorts with harmonized high-density protocols, rigorous artifact control, and linkage to clinical milestones. Such infrastructure will enable the qualification of alpha-band restoration, delta-band hypersynchrony, and neurovascular coupling as pharmacodynamic readouts, fostering precision monitoring and network-targeted therapy in Huntington’s disease. Full article

Wireless Sensor Networks (WSNs) consist of distributed sensor nodes that collect and transmit environmental data, often in resource-constrained and unsecured environments. These characteristics make WSNs highly vulnerable to various security threats. To address this, the objective of this research is to design and evaluate a deep learning-based Intrusion Detection System (IDS) that is both accurate and efficient for real-time threat detection in WSNs. This study proposes a hybrid IDS model combining one-dimensional Convolutional Neural Networks (Conv1Ds), Gated Recurrent Units (GRUs), and Self-Attention mechanisms. A Conv1D extracts spatial features from network traffic, GRU captures temporal dependencies, and Self-Attention emphasizes critical sequence components, collectively enhancing detection of subtle and complex intrusion patterns. The model was evaluated using the WSN-DS dataset and demonstrated superior performance compared to traditional machine learning and simpler deep learning models. It achieved an accuracy of 98.6%, precision of 98.63%, recall of 98.6%, F1-score of 98.6%, and an ROC-AUC of 0.9994, indicating strong predictive capability even with imbalanced data. In addition to centralized training, the model was tested under cooperative, node-based learning conditions, where each node independently detects anomalies and contributes to a collective decision-making framework. This distributed approach improves detection efficiency and robustness. The proposed IDS offers a scalable and resilient solution tailored to the unique challenges of WSN security. Full article

Air pollution in urban areas has increased significantly over the past few years due to industrialization and population increase. Therefore, accurate predictions are needed to minimize their impact. This paper presents a neural network-based examination for forecasting Air Quality Index (AQI) values, employing two different models: a variable-depth neural network (NN) called slideNN, and a Gated Recurrent Unit (GRU) model. Both models used past particulate matter measurements alongside local meteorological data as inputs. The slideNN variable-depth architecture consists of a set of independent neural network models, referred to as strands. Similarly, the GRU model comprises a set of independent GRU models with varying numbers of cells. Finally, both models were combined to provide a hybrid cloud-based model. This research examined the practical application of multi-strand neural networks and multi-cell recurrent neural networks in air quality forecasting, offering a hands-on case study and model evaluation for the city of Ioannina, Greece. Experimental results show that the GRU model consistently outperforms the slideNN model in terms of forecasting losses. In contrast, the hybrid GRU-NN model outperforms both GRU and slideNN, capturing additional localized information that can be exploited by combining particle concentration and microclimate monitoring services. Full article

Common-mode analysis (CMA) is a qualitative analytical method used to support the evaluation of independence in the system safety assessment of civil aircraft. In traditional CMA, independence requirements are usually identified by evaluating the combination of events using the fault tree AND-gates. This approach is cumbersome and highly dependent on the skills and experiences of system safety engineers. An Architecture Analysis and Design Language (AADL)-based methodology is proposed to derive independence requirements for CMA. Error propagation data in AADL is extracted to develop a fault propagation model. Subsequently, potential factors contributing to common-mode failures (CMFs) are identified using the fault propagation model. A Primary Flight Computer (PFC) of an aircraft is used as a case study to illustrate the effectiveness of our proposed method. Full article

In today’s business environment, emails are essential across all sectors, including finance and academia. There are two main types of emails: ham (legitimate) and spam (unsolicited). Spam wastes consumers’ time and resources and poses risks to sensitive data, with volumes doubling daily. Current spam identification methods, such as Blocklist approaches and content-based techniques, have limitations, highlighting the need for more effective solutions. These constraints call for detailed and more accurate approaches, such as machine learning (ML) and deep learning (DL), for realistic detection of new scams. Emphasis has since been placed on the possibility that ML and DL technologies are present in detecting email spam. In this work, we have succeeded in developing a hybrid deep learning model, where Long Short-Term Memory (LSTM) and the Gated Recurrent Unit (GRU) are applied distinctly to identify spam email. Despite the fact that the other models have been applied independently (CNNs, LSTM, GRU, or ensemble machine learning classifier) in previous studies, the given research has provided a contribution to the existing body of literature since it has managed to combine the advantage of LSTM in capturing the long-term dependency and the effectiveness of GRU in terms of computational efficiency. In this hybridization, we have addressed key issues such as the vanishing gradient problem and outrageous resource consumption that are usually encountered in applying standalone deep learning. Moreover, our proposed model is superior regarding the detection accuracy (90%) and AUC (98.99%). Though Transformer-based models are significantly lighter and can be used in real-time applications, they require extensive computation resources. The proposed work presents a substantive and scalable foundation to spam detection that is technically and practically dissimilar to the familiar approaches due to the powerful preprocessing steps, including particular stop-word removal, TF-IDF vectorization, and model testing on large, real-world size dataset (Enron-Spam). Additionally, delays in the feature comparison technique within the model minimize false positives and false negatives. Full article

This study investigates the performance of temporal deep learning models with attention mechanisms for crop classification using Sentinel-1 C-band synthetic aperture radar (C-SAR) data. A time series of 16 scenes, acquired at 12-day intervals from 25 April to 22 October 2024, was used to classify six crop types: beans, beetroot, grassland, maize, potato, and winter wheat. Three temporal models—long short-term memory (LSTM), bidirectional gated recurrent unit (Bi-GRU), and temporal convolutional network (TCN)—were evaluated with and without an attention mechanism. All model configurations achieved accuracies above 83%, demonstrating the potential of Sentinel-1 SAR data for reliable, weather-independent crop classification. The TCN with attention model achieved the highest accuracy of 85.7%, significantly outperforming the baseline. LSTM also showed improved accuracy when combined with attention, whereas Bi-GRU did not benefit from the attention mechanism. These results highlight the effectiveness of combining temporal deep learning models with attention mechanisms to enhance crop classification using Sentinel-1 SAR time-series data. This study further confirms that freely available, regularly acquired Sentinel-1 observations are well-suited for robust crop mapping under diverse environmental conditions. Full article

Accurate traffic forecasting is challenging due to the complex spatial-temporal interdependencies of large road networks and sudden speed changes caused by unexpected events. Traditional models often struggle with the non-stationary and volatile characteristics of traffic time series. While existing sequence decomposition methods can capture stable long-term trends and periodic information, they fail to address complex fluctuation patterns. To tackle this issue, we propose the Spatial-Temporal traffic flow prediction with residual and trend Decomposition Transformer (STDformer), which decomposes time series into different components, thus enabling more accurate modeling of both short-term and long-term dependencies. Our method processes the time series in parallel using the Trend Decomposition Block and the Spatial-Temporal Relation Attention. The Spatial-Temporal Relation Attention captures dynamic spatial correlations across the road network, while the Trend Decomposition Block decomposes the series into trend, seasonal, and residual components. Each component is then independently modeled by the Temporal Modeling Block to capture its unique temporal dynamics. Finally, the outputs from the Temporal Modeling Block are fused through a selective gating mechanism, combined with the Spatial-Temporal Relation Attention output to produce the final prediction. Extensive experiments on PEMS traffic datasets demonstrate that STDformer consistently outperforms state-of-the-art traffic flow prediction methods, particularly under volatile conditions. These results validate STDformer’s practical utility in real-world traffic management, highlighting its potential to assist traffic managers in making informed decisions and optimizing traffic efficiency. Full article

Introduction: Combat sport athletes are exposed to repetitive head impacts yet also develop distinct performance-related brain adaptations. Electroencephalography (EEG) provides millisecond-level insight into both processes; however, findings are dispersed across decades of heterogeneous studies. This mechanistic review consolidates and interprets EEG evidence to elucidate how participation in combat sports shapes brain function and to identify research gaps that impede clinical translation. Methods: A structured search was conducted in March 2025 across PubMed/MEDLINE, Scopus, Cochrane Library, ResearchGate, Google Scholar, and related databases for English-language clinical studies published between January 1980 and March 2025. Eligible studies recorded raw resting or task-related EEG in athletes engaged in boxing, wrestling, judo, karate, taekwondo, kickboxing, or mixed martial arts. Titles, abstracts, and full texts were independently screened by two reviewers. Twenty-three studies, encompassing approximately 650 combat sport athletes and 430 controls, met the inclusion criteria and were included in the qualitative synthesis. Results: Early visual EEG and perfusion studies linked prolonged competitive exposure in professional boxers to focal hypoperfusion and low-frequency slowing. More recent quantitative studies refined these findings: across boxing, wrestling, and kickboxing cohorts, chronic participation was associated with reduced alpha and theta power, excess slow-wave activity, and disrupted small-world network topology—alterations that often preceded cognitive or structural impairments. In contrast, elite athletes in karate, fencing, and kickboxing consistently demonstrated neural efficiency patterns, including elevated resting alpha power, reduced task-related event-related desynchronization (ERD), and streamlined cortico-muscular coupling during cognitive and motor tasks. Acute bouts elicited transient increases in frontal–occipital delta and high beta power proportional to head impact count and cortisol elevation, while brief judo chokes triggered short-lived slow-wave bursts without lasting dysfunction. Methodological heterogeneity—including variations in channel count (1 to 64), reference schemes, and frequency band definitions—limited cross-study comparability. Conclusions: EEG effectively captures both the adverse effects of repetitive head trauma and the cortical adaptations associated with high-level combat sport training, underscoring its potential as a rapid, portable tool for brain monitoring. Standardizing acquisition protocols, integrating EEG into longitudinal multimodal studies, and establishing sex- and age-specific normative data are essential for translating these insights into practical applications in concussion management, performance monitoring, and regulatory policy. Full article

Upper limb assistive exoskeletons help stroke patients by assisting arm movement in impaired individuals. However, effective control of these systems to help stroke survivors is a complex task. In this paper, a novel approach is proposed to enhance the control of upper limb assistive exoskeletons by using torque estimation and prediction in a proportional–integral–derivative (PID) controller loop to more optimally integrate the torque of the exoskeleton robot, which aims to eliminate system uncertainties. First, a model for torque estimation from Electromyography (EMG) signals and a predictive torque model for the upper limb exoskeleton robot for the elbow are trained. The trained data consisted of two-dimensional high-density surface EMG (HD-sEMG) signals to record myoelectric activity from five upper limb muscles (biceps brachii, triceps brachii, anconeus, brachioradialis, and pronator teres) during voluntary isometric contractions for twelve healthy subjects performing four different isometric tasks (supination/pronation and elbow flexion/extension) for one minute each, which were trained on long short-term memory (LSTM), bidirectional LSTM (BLSTM), and gated recurrent units (GRU) deep neural network models. These models estimate and predict torque requirements. Finally, the estimated and predicted torque from the trained network is used online as input to a PID control loop and robot dynamic, which aims to control the robot optimally. The results showed that using the proposed method creates a strong and innovative approach to greater independence and rehabilitation improvement. Full article

As a novel energy-saving and maneuvering device for ships, the gate rudder system (GRS) functions similarly to an accelerating duct. While providing additional thrust, its independently controllable rudder blades on either side of the propeller also enhance ship maneuverability. The GRS was first fully implemented on a container ship in Japan, demonstrating improved propulsion efficiency, fuel savings, and excellent performance in maneuvering, noise, and vibration reduction. In recent years, extensive research has been conducted on the hydrodynamic performance, acoustic characteristics, and energy-saving effects of the GRS. However, certain gaps remain in the research, such as a lack of systematic studies on optimal GRS design in the publicly available literature. Only Ahmet Yusuf Gurkan has investigated the sensitivity of propulsion performance to parameters such as rudder angle, rudder X-shift, rudder tip skewness, and blade tip chord ratio. Therefore, this study employs the JBC benchmark vessel and adopts a coupled CFD-CAESES approach to develop a matching optimization design for the GRS. The influence of geometric parameters—including GRS airfoil camber, maximum camber position, chord length, thickness, distance from the leading edge to the propeller plane, and the gap between the GRS and propeller blades—on ship propulsion performance is investigated. The sensitivity of these design variables to propulsion performance is analyzed, and the optimal GRS design is selected to predict and evaluate its energy-saving effects. This research establishes a rapid and comprehensive CFD-based optimization methodology for GRS matching design. The findings indicate that the gap between the GRS and propeller, the distance from the GRS to the stern, and the airfoil camber of the GRS significantly contribute to various performance responses. After GRS installation, the viscous pressure resistance of the JBC ship decreases, resulting in an 8.05% energy-saving effect at the designated speed. Full article