Search Results (29)

Coded aperture imaging (CAI) is a powerful imaging technology that has rapidly developed during the past decade. CAI technology and its integration with incoherent holography have led to the development of several cutting-edge imaging tools, devices, and techniques with widespread interdisciplinary applications, such as in astronomy, biomedical sciences, and computational imaging. In this review, we provide a comprehensive overview of the recently developed CAI techniques in the framework of incoherent digital holography. The review starts with an overview of the milestones in modern CAI technology, such as interferenceless coded aperture correlation holography, followed by a detailed survey of recently developed CAI techniques and system designs in subsequent sections. Each section provides a general description, principles, potential applications, and associated challenges. We believe that this review will act as a reference point for further advancements in CAI technologies. Full article

A portable incoherent digital holography system without a polarization filter or a refractive lens was developed. Phase-shifted self-interference incoherent holograms of light diffracted from an object were generated without attenuation due to a polarization filter using two polarization-sensitive phase-only spatial light modulators (TPP-SLMs). The number of optical elements in filter-free lens-free incoherent digital holography was reduced to make the system compact and portable. Experiments were conducted using the developed digital holography system set on a tripod stand and objects illuminated by a light-emitting diode. Full article

Coded aperture imaging (CAI) is a well-established computational imaging technique consisting of two steps, namely the optical recording of an object using a coded mask, followed by a computational reconstruction using a computational algorithm using a pre-recorded point spread function (PSF). In this tutorial, we introduce a simple yet elegant technique called spatial ensemble mapping (SEM) for CAI that allows us to tune the axial resolution post-recording from a single camera shot recorded using an image sensor. The theory, simulation studies, and proof-of-concept experimental studies of SEM-CAI are presented. We believe that the developed approach will benefit microscopy, holography, and smartphone imaging systems. Full article

Incoherent digital holography is a promising solution for acquiring three-dimensional information in the form of interference without a laser. Self-interference is the principal phenomenon of incoherent holography which splits the incident light wave into two waves and interferes with each one. The characteristics of incoherent holograms are defined by the optical configuration of wavefront dividing units. In this paper, we propose the synthetic aperture method to achieve super-resolution of geometric phase lens-based self-interference incoherent digital holography. We analyze the resolution of incoherent holograms acquired from geometric phase lenses and experimentally demonstrate the resolution enhancement via the synthetic-aperture method. Moreover, the sparse synthetic-aperture method is proposed to ensure effective data capturing and the numerical optimization method is also proposed to fill the gap between the empty space of sparsely acquired holograms. Full article

This work studies enhancing the capabilities of compact laser spectroscopes integrated into space-exploration rovers by adding 3D topography measurement techniques. Laser spectroscopy enables the in situ analysis of sample composition, aiding in the understanding of the geological history of extraterrestrial bodies. To complement spectroscopic data, the inclusion of 3D imaging is proposed to provide unprecedented contextual information. The morphological information aids material characterization and hence the constraining of rock and mineral histories. Assigning height information to lateral pixels creates topographies, which offer a more complete spatial dataset than contextual 2D imaging. To aid the integration of 3D measurement into future proposals for rover-based laser spectrometers, the relevant scientific, rover, and sample constraints are outlined. The candidate 3D technologies are discussed, and estimates of performance, weight, and power consumptions guide the down-selection process in three application examples. Technology choice is discussed from different perspectives. Inline microscopic fringe-projection profilometry, incoherent digital holography, and multiwavelength digital holography are found to be promising candidates for further development. Full article

Holographic techniques are indispensable tools for modern optical engineering. Over the past two decades, research about incoherent digital holography has continued to attract attention. Optical scanning holography (OSH) can obtain incoherent holograms using single-pixel detection and structured illumination with Fresnel zone patterns (FZPs). Particularly by changing the size of a detector, OSH can also obtain holograms under coherently illuminated conditions. Since 1979, OSH has continuously evolved. According to the evolution of semiconductor technology, spatial light modulators (SLMs) come to be useful for various imaging fields. By using SLM techniques for OSH, the practicality of OSH is improved. These SLM-based OSH methods are termed computational OSH (COSH). In this review, the configurations, recording and reconstruction methods, and proposed applications of COSH are reviewed. Full article

Spatial-structured longitudinal light beams are optical fields sculpted in three-dimensional (3D) space by diffractive optical elements. These beams have been recently suggested for use in improving several imaging capabilities, such as 3D imaging, enhancing image resolution, engineering the depth of field, and sectioning 3D scenes. All these imaging tasks are performed using coded aperture correlation holography systems. Each system designed for a specific application is characterized by a point spread function of a different spatial-structured longitudinal light beam. This article reviews the topic of applying certain structured light beams for optical imaging. Full article

Light sheet microscopy in live cells requires minimal excitation intensity and resolves three-dimensional (3D) information rapidly. Lattice light sheet microscopy (LLSM) works similarly but uses a lattice configuration of Bessel beams to generate a flatter, diffraction-limited z-axis sheet suitable for investigating subcellular compartments, with better tissue penetration. We developed a LLSM method for investigating cellular properties of tissue in situ. Neural structures provide an important target. Neurons are complex 3D structures, and signaling between cells and subcellular structures requires high resolution imaging. We developed an LLSM configuration based on the Janelia Research Campus design or in situ recording that allows simultaneous electrophysiological recording. We give examples of using LLSM to assess synaptic function in situ. In presynapses, evoked Ca²⁺ entry causes vesicle fusion and neurotransmitter release. We demonstrate the use of LLSM to measure stimulus-evoked localized presynaptic Ca²⁺ entry and track synaptic vesicle recycling. We also demonstrate the resolution of postsynaptic Ca²⁺ signaling in single synapses. A challenge in 3D imaging is the need to move the emission objective to maintain focus. We have developed an incoherent holographic lattice light-sheet (IHLLS) technique to replace the LLS tube lens with a dual diffractive lens to obtain 3D images of spatially incoherent light diffracted from an object as incoherent holograms. The 3D structure is reproduced within the scanned volume without moving the emission objective. This eliminates mechanical artifacts and improves temporal resolution. We focus on LLS and IHLLS applications and data obtained in neuroscience and emphasize increases in temporal and spatial resolution using these approaches. Full article

Pattern recognition techniques form the heart of most, if not all, incoherent linear shift-invariant systems. When an object is recorded using a camera, the object information is sampled by the point spread function (PSF) of the system, replacing every object point with the PSF in the sensor. The PSF is a sharp Kronecker Delta-like function when the numerical aperture (NA) is large with no aberrations. When the NA is small, and the system has aberrations, the PSF appears blurred. In the case of aberrations, if the PSF is known, then the blurred object image can be deblurred by scanning the PSF over the recorded object intensity pattern and looking for pattern matching conditions through a mathematical process called correlation. Deep learning-based image classification for computer vision applications gained attention in recent years. The classification probability is highly dependent on the quality of images as even a minor blur can significantly alter the image classification results. In this study, a recently developed deblurring method, the Lucy-Richardson-Rosen algorithm (LR²A), was implemented to computationally refocus images recorded in the presence of spatio-spectral aberrations. The performance of LR²A was compared against the parent techniques: Lucy-Richardson algorithm and non-linear reconstruction. LR²A exhibited a superior deblurring capability even in extreme cases of spatio-spectral aberrations. Experimental results of deblurring a picture recorded using high-resolution smartphone cameras are presented. LR²A was implemented to significantly improve the performances of the widely used deep convolutional neural networks for image classification. Full article

We previously developed an incoherent holography technique for use in lattice light sheet (LLS) microscopes that represents a specialized adaptation of light sheet microscopy. Light sheet instruments resolve 3D information by illuminating the sample at 90° to the imaging plane with a sheet of laser light that excites fluorophores in the sample only in a narrow plane. Imaging this plane and then moving it in the imaging z-axis allows construction of the sample volume. Among these types of instruments, LLS microscopy gives higher z-axis resolution and tissue depth penetration. It has a similar working principle to light sheet fluorescence microscopy but uses a lattice configuration of Bessel beams instead of Gaussian beams. Our incoherent light detection technique replaces the glass tube lens of the original LLS with a dual diffractive lens system to retrieve the axial depth of the sample. Here, we show that the system is applicable to all light sheet instruments. To make a direct comparison in the same emission light path, we can imitate the nature of non-Bessel light sheet systems by altering the mask annuli used to create Bessel beams in the LLS system. We change the diffractive mask annuli from a higher NA anulus to a smaller NA anulus. This generates a Gaussian excitation beam similar to conventional light sheet systems. Using this approach, we propose an incoherent light detection system for light sheet 3D imaging by choosing a variable NA and moving only the light sheet while keeping the sample stage and detection microscope objective stationary. Full article

Incoherent digital holography (IDH) is a technique used to obtain a three-dimensional (3D) image of spatially incoherent light diffracted from an object as an incoherent hologram. Color holographic 3D motion picture imaging of daily-use light at the frame rate of a color polarization imaging camera can be achieved by the combination of IDH and single-shot phase-shifting interferometry. We show experimental results for color 3D motion picture imaging in this proceedings article. Full article

Optical imaging has been utilized in nature and technology for decades. Recently, new methods of optical imaging assisted by computational imaging techniques have been proposed and demonstrated. We describe several new methods of three-dimensional optical imaging, from Fresnel incoherent correlation holography (FINCH) to interferenceless coded aperture correlation holography (COACH). FINCH and COACH are methods for recording digital holograms of a three-dimensional scene. However, COACH can be used for other incoherent and coherent optical applications. The possible applications for these imaging methods, ranging from a new generation of fluorescence microscopes to noninvasive imaging methods through a scattering medium, are mentioned. Full article

Conventional digital holography uses the technique of combining two coherent light fields and the numerical reconstruction of the recorded hologram leads to the object amplitude and phase information. Despite significant developments in the DH with coherent light, complex field imaging with arbitrary coherent sources is also desired for various reasons. Here, we present a possible experimental approach for holography with incoherent light. In the case of incoherent light, the complex spatial coherence function is a measurable quantity and the incoherent object holograms are recorded as the coherence function. Thus, to record complex spatial coherence a square Sagnac radial shearing interferometer is designed with the phase-shifting approach. The five-step phase-shifting method helps to measure the fringe visibility and the corresponding phase, which jointly represents the complex coherence function. The inverse Fourier transform of the complex coherence function helps to retrieve the object information. Full article

Direct imaging systems that create an image of an object directly on the sensor in a single step are prone to many constraints, as a perfect image is required to be recorded within this step. In designing high resolution direct imaging systems with a diffractive lens, the outermost zone width either reaches the lithography limit or the diffraction limit itself, imposing challenges in fabrication. However, if the imaging mode is switched to an indirect one consisting of multiple steps to complete imaging, then different possibilities open. One such method is the widely used indirect imaging method with Golay configuration telescopes. In this study, a Golay-like configuration has been adapted to realize a large-area diffractive lens with three sub-aperture diffractive lenses. The sub-aperture diffractive lenses are not required to collect light and focus them to a single point as in a direct imaging system, but to focus independently on different points within the sensor area. This approach of a Large-Area Diffractive lens with Integrated Sub-Apertures (LADISA) relaxes the fabrication constraints and allows the sub-aperture diffractive elements to have a larger outermost zone width and a smaller area. The diffractive sub-apertures were manufactured using photolithography. The fabricated diffractive element was implemented in indirect imaging mode using non-linear reconstruction and the Lucy–Richardson–Rosen algorithm with synthesized point spread functions. The computational optical experiments revealed improved optical and computational imaging resolutions compared to previous studies. Full article