Search Results (6,598)

Introduction: Mediterranean coastal wetlands are highly dynamic ecosystems that support diverse fish communities and are often of high conservation value. The Ebro Delta is one of the most important coastal wetlands in the Western Mediterranean, and knowledge of fish assemblages is essential for its effective conservation and management. Environmental DNA (eDNA) metabarcoding provides a non-invasive approach that can potentially complement conventional surveys for fish biodiversity monitoring. Objective: This study aimed to evaluate the potential of eDNA metabarcoding as a complementary tool to conventional fyke net surveys for fish biodiversity monitoring in the Mediterranean coastal wetlands. Methodology: In 2022, fish assemblages were surveyed across 12 areas of the Ebro Delta using eDNA metabarcoding (12S MiFish) and conventional fyke net sampling. Results were compared with a 22-year historical dataset. Results: A total of 27 fish taxa were detected, 13 of which were exclusive to eDNA, 11 were shared between methods, and three were recorded only by fyke nets. The reliability of eDNA metabarcoding was supported by the detection of endangered species, such as Anguilla anguilla and Apricaphanius iberus; ubiquitous taxa, such as Atherina boyeri and Pomatoschistus microps; and invasive species, such as Gambusia holbrooki and Cyprinus carpio. Detection of invasive species was maximized using eDNA. While eDNA revealed higher species richness than fyke nets, community composition differed significantly between methods, reflecting distinct detection patterns. eDNA preferentially detected non-benthic species, whereas fyke nets were more robust for benthic taxa detection. eDNA recovered most historically recorded species but failed to detect some taxa, such as Misgurnus anguillicaudatus and Sardina pilchardus. Despite richness differences, the two methods provided complementary views of fish assemblages, highlighting method-specific detection limitations and opportunities. Conclusions: eDNA does not fully replace conventional surveys and their combined use improves the detection of threatened and invasive species, better supporting conservation and management. Full article

Longnan, located in Gansu Province, China, at the junction of Shaanxi, Gansu, and Sichuan provinces, represents one of the key corridors of the Northern Tea-Horse Ancient Road. This region preserves abundant traditional timber covered bridges with distinct local characteristics. This study employs ArcGIS spatial analysis and documentary research methods to explore the spatial distribution, spatiotemporal evolution, and influencing factors of these bridges. Spatial analyses (nearest neighbor index, kernel density, and standard deviational ellipse) are based on 71 bridges with traceable coordinates, while the temporal evolution analysis incorporates 80 bridges (64 with definite construction periods and 16 with unknown dates; the latter are handled through a sensitivity analysis as described later in this paper The results indicate that the timber covered bridges in Longnan exhibit a significantly clustered distribution, presenting a pattern of “dense in the southwest and sparse in the northeast”, with Wen County and Kang County as the core clustering areas. Temporally, they follow a unimodal evolution pattern: initiation in the Ming Dynasty, peak in the Qing Dynasty, decline in the Republic of China period, and near stagnation in modern times. The location and distribution of the covered bridges show a strong statistical association with natural conditions (e.g., topography, hydrology) and exhibit spatial coincidence with modern vegetation coverage—the latter treated solely as a contemporary context variable rather than a historical driver. Spatial coincidence with the ancient road is quantified (60.56% within a 2000 m buffer), while settlement proximity is only qualitatively noted as background. Socio-economic factors (e.g., population, transportation, and settlements) are examined qualitatively and display spatial coincidence rather than quantitatively measured influence; these factors cannot be directly compared with natural factors. Full article

Land-use change from natural vegetation to agricultural systems significantly affects watershed hydrology and water quality. This study assesses the long-term effects of historical land-use change on hydrologic processes and nitrogen transport in the Straight River watershed, Minnesota, USA, using the Soil and Water Assessment Tool Plus (SWAT+) model. Three land-use scenarios were created to assess changes in water balance and nitrate levels. These scenarios represent the reconstructed pre-settlement conditions from 1855, established agricultural development from 2006, and current conditions from 2022. Results show a significant increase in water percolation and groundwater recharge. Percolation more than doubled, increasing from about 118 mm under reconstructed pre-colonial conditions to over 256 mm in 2022. Streamflow increased to 2.1 m³s⁻¹ in 2022, indicating improved hydrologic connectivity and groundwater contributions. Nitrate leaching increased from about 1.14 kg N ha⁻¹ to more than 32 kg N ha⁻¹ (1850s–2022), and nitrate export increased by >2000%, indicating strong nitrate loading. The significant increase in nitrate compared to water fluxes points to agriculture as the primary source of groundwater pollution and downstream nutrient loading. These findings highlight the importance of land-use change in affecting water balance and nutrient behavior. They also point out the need to include a historical baseline in watershed assessments. The results show the importance of better land and nutrient management strategies to reduce nitrate losses and protect water resources in intensively managed agricultural areas. Full article

Façades play a decisive role in shaping the visual and symbolic character of historic urban environments. Recent European funding schemes promoting energy-efficient retrofitting have accelerated interventions on building envelopes. Although aligned with decarbonization objectives, these processes are generating significant chromatic and material transformations that risk eroding the visual coherence and cultural sustainability of consolidated urban areas. In the historic Ensanches of San Sebastián, the replacement of traditional envelope systems with new cladding solutions is leading to the loss of the architectural style of some facades and altering their materials, textures, and colors. A progressive “contagion effect” has been identified, whereby dissonant chromatic schemes—often associated with the proliferation of so-called “zebra blocks”, residential buildings with façades clad in alternating black and white stripes that have proliferated in recent urban developments—are replicated across adjacent buildings, gradually weakening spatial continuity and the genius loci of the neighborhood. In response to this phenomenon, this research develops a systematic methodology to analyze, quantify, and anticipate chromatic transformation in consolidated urban fabrics. The study combines historical morphological analysis, classification of architectural periods, and chromatic mapping of recent façade interventions. Based on this framework, a CARI, Chromatic Alteration Risk Index is proposed to evaluate the potential impact of façade alterations on urban chromatic coherence. Drawing on an epidemiological framework, the methodology enables the identification of critical transformation clusters, the assessment of contagion dynamics, and the definition of regulatory thresholds for color and material interventions. By integrating perceptual criteria, urban morphology, and spatial distribution patterns, the study moves beyond descriptive diagnosis and offers a transferable tool for municipal planning. The proposed approach supports the proactive regulation of façade rehabilitation processes, balancing energy efficiency objectives with the preservation of collective memory, material identity, and urban sensory quality. This study proposes a quantitative model of “urban chromatic contagion” to assess how façade color interventions propagate within a neighborhood. We define the Chromatic Integration Percentage (CIP) and the Chromatic Alteration Risk Index (CARI) of the analyzed area. Results indicate that poorly regulated façades show higher chromatic dissonance (low CIP) and act as contagion hotspots, while a clear risk gradient emerges: highly protected buildings present lower risk, whereas mixed typologies and recent rehabilitations concentrate higher CARI values. The model supports preventive urban color management by identifying areas at risk before visible alteration. Full article

Currently, in Catalonia, Spanish toothcarp (Apricaphanius iberus) has a discontinuous distribution, with its main populations located in the Ebro Delta and the Empordà wetlands. Along the Catalan coast, there are some small, isolated populations, most of which have been recently introduced. In the Empordà area, this species still maintains three isolated populations within two natural parks, where it currently occupies the northernmost site of its global distribution. Between 2017 and 2025, several fish sampling campaigns were carried out in the Empordà wetlands (Northeast Catalonia), gradually covering the entire area of potential distribution of this species in the area. In total, 228 points have been sampled, at least in one occasion, in all types of water bodies (rivers, canals, lagoons, marshes). In 106 points, more than two sampling events have been performed. Furthermore, in 2025, a specific diagnosis was carried out to better understand the current situation of the species in these protected spaces after a prolonged and severe drought. Sampling was carried out everywhere with fykenets, adding the use of nets and electrofishing in some stations located in large bodies of water. The known historical evolution of Spanish toothcarp distribution in the Empordà has been presented since the first data was made available at the beginning of the 1980s of the 20th century until now. Today, this fish only occupies approximately 10% of its potential distribution in the area. Over the last five decades, its distribution has expanded and contracted several times, but in 2025, it was once again in the worst possible situation of the analysis period. Therefore, the implementation of protected areas, or the execution of several large ecological restoration projects, have only succeeded in maintaining existing populations, but not in the expansion of its potential distribution to unoccupied areas. The main factors that explain this general situation are mainly (1) the proliferation of exotic species, (2) the loss of ecological status, and (3) hydrological alterations and increasingly severe droughts. Thus, the recovery and long-term conservation of Spanish toothcarp in the Empordà area inevitably requires the implementation of more extensive and decisive measures to reverse the incidence of these factors. Full article

Baseline information on terrestrial wildlife communities and their activity patterns is essential for protected-area management, but such information remains limited for Hunan Zhangjiajie Giant Salamander National Nature Reserve, where conservation attention has historically focused on the Chinese giant salamander and associated aquatic ecosystems. From March 2024 to August 2025, we conducted a camera-trap survey in broad-leaved and coniferous forest habitats of the reserve to document terrestrial mammals and ground-dwelling birds, evaluate taxonomic completeness, and describe diel and seasonal activity patterns. Across 43 camera-trap stations and 16,314 effective camera-trap days, we recorded 59 wildlife species, including 18 mammals and 41 ground-dwelling birds. The assemblage included nationally protected, threatened, and Chinese endemic species, indicating that the reserve’s forest habitats support important terrestrial biodiversity in addition to its aquatic conservation target. Taxonomic completeness curves suggested that the current survey captured most camera-detectable mammal and ground-dwelling bird taxa under the present sampling design, although the results should not be interpreted as a complete inventory of the reserve’s total vertebrate diversity. Annual diel activity analysis of 11 focal species showed clear temporal differentiation among ecological groups: small and medium-sized carnivores were mainly nocturnal, ground-dwelling birds, and red-hipped squirrel were primarily diurnal, and ungulates showed mixed or crepuscular-to-nocturnal tendencies. Seasonal analyses based on bioclimatic periods showed interspecific differences in activity-density distributions between the cool-dry and warm-wet seasons. However, peak-shift reliability analysis indicated that most focal species retained broadly similar main activity peaks across seasons; masked palm civet was the only species showing reliable seasonal displacement of its main activity peak. Pairwise temporal overlap analyses described temporal co-occurrence patterns among selected sympatric species but should not be interpreted as evidence of direct interaction or niche differentiation. Overall, this study provides baseline data on camera-detected terrestrial vertebrates in the reserve and supports long-term monitoring, forest habitat management, and disturbance control for terrestrial mammals and ground-dwelling birds. Full article

Flooding triggered by intense precipitation is a significant natural hazard affecting Mediterranean regions, where complex terrain, rapid hydrological response and increasing urbanization can amplify flood impacts. This study assesses flood susceptibility in two representative Mediterranean River catchments: the Koiliaris in Crete, Greece, and the Pediaios in Cyprus. A compact Flood Hazard Index (FHI) was developed by integrating the Topographic Wetness Index (TWI), Curve Number (CN), and R20 heavy rain frequency index, representing the principal geomorphological, hydrological and climatological controls of flood generation. Spatial datasets including EU-DEM elevation data, CORINE land cover, European soil databases, and Copernicus CERRA precipitation reanalysis were combined within a GIS-based multi-criteria framework using Analytic Hierarchy Process weighting. The resulting FHI maps identify high flood susceptibility along river corridors, low-lying accumulation zones, and urbanized areas. In the Koiliaris basin, 34% of the area fell within the high and very high susceptibility classes, mainly in downstream alluvial zones, whereas in the Pediaios basin, 29% of the area fell within the high and very high susceptibility classes, concentrated around the urbanized Nicosia corridor. The analysis of historical flood events provided a qualitative consistency assessment of the FHI patterns, acknowledging that the absence of spatially explicit flood-inundation footprints limits quantitative validation. Full article

Dental fluorosis (DF) remains a global public health challenge traditionally attributed to elevated water fluoride F⁻. However, the Halo Effect and environmental factors now complicate this dose–response relationship. Following PRISMA 2020 guidelines, this systematic review identified 20 observational studies (n = 21,780) via PubMed, Scopus, and Web of Science. Inclusion logic utilized the PICOS framework, specifically selecting human studies that reported quantitative water F⁻ levels alongside environmental or dietary confounders. Quality was assessed via the Newcastle–Ottawa Scale. Synthesis revealed that in optimal fluoridated areas (0.7 mg/L), mild DF prevalence reached 15–20% in cohorts with high “Halo Effect” exposure (infant formula, processed beverages) a twofold increase over historical benchmarks. High altitude (>2000 m) and arid climates further exacerbated toxicity by altering renal clearance. These factors sustain systemic fluoride levels that inhibit protease activity (MMP-20/KLK4) and induce endoplasmic reticulum stress during enamel maturation, causing hypomineralization. Current water-centric monitoring is insufficient for modern risk assessment. A transition toward Total Daily Intake (TDI) models and context-specific standards accounting for altitude and dietary diffusion is essential to balance caries prevention with systemic safety. Full article

Introduction: Freshwater fish communities in Mediterranean basins have undergone profound changes over recent decades due to biological invasions, habitat alteration, and hydrological regulation. The Segura River basin (SE Spain), particularly in the Region of Murcia, represents a paradigmatic case of these transformations, with increasing pressures on native ichthyofauna and growing relevance for recreational fisheries. Objective: This study aims to provide an updated inventory of fish communities in the rivers and reservoirs of the Region of Murcia, assessing current composition, relative abundance, and conservation status. Methodology: Fish assemblages were surveyed during sampling campaigns conducted in autumn 2023, 2024 and 2025. Sampling sites included representative reservoirs and river reaches within the middle sector of the Segura River basin, focusing on areas of interest for recreational fishing. Passive fishing gears were used in reservoirs, while electrofishing was conducted in riverine habitats. Presence and relative abundance data were recorded for all detected taxa. Results: A total of 15 taxa were identified (8 in reservoirs and 15 in river reaches). Reservoir communities were almost entirely dominated by non-native invasive species, including Cyprinus carpio, Alburnus alburnus, Sander lucioperca, Micropterus salmoides, Lepomis gibbosus, and Gambusia holbrooki, with Luciobarbus sclateri as the only native species. Riverine sections of the main channel also showed a strong dominance of exotic taxa, with additional species such as Esox lucius, Oncorhynchus mykiss, Gobio lozanoi, and Pseudochondrostoma polylepis. Native species detected included L. sclateri (dominant), Anguilla anguilla, and Salariopsis fluviatilis, the latter showing a recent expansion likely linked to human-mediated introduction. Isolated tributaries hosted the most valuable assemblages, including populations of L. sclateri, Squalius tartessicus, and the endangered Valencia hispanica (recently unauthorized translocations into the Segura river basin). Conclusions: Current fish communities in the Region of Murcia reflect a marked ecological degradation compared to historical conditions, driven by the proliferation of non-native species and habitat alteration. Changes over the last 30 years have been particularly pronounced in reservoirs and regulated sections of the main river channel, where local extinction of the native S. tartessicus has also been detected. Only the headwaters of certain tributaries retain fish assemblages of notable conservation interest, highlighting their priority for management and protection. Full article

Wikidata provides extensive coverage of tourism-related Points of Interest (POIs), yet its heterogeneous type system and uneven metadata limit its direct use in smart tourism applications. This paper presents an end-to-end pipeline that transforms Wikidata POIs into a compact and interpretable tourism-oriented representation supporting multi-category assignments. We collect POIs from six countries—Greece, Italy, Spain, Norway, Sweden, and Denmark—and construct a dataset that integrates core identifiers with textual descriptions, type information, heritage indicators, geographic coordinates, and Wikipedia sitelinks. We introduce an eight-category tourism taxonomy capturing key themes, including cultural venues, archaeological and historic sites, monuments, fortifications, religious sites, protected areas, natural features, and coastal or water locations. As a reproducible baseline, category likelihoods are estimated using sentence embeddings and similarity to category anchor descriptions, producing a probability vector for each POI. Building on this baseline, we propose a fuzzy inference layer that integrates embedding-based probabilities with structured Wikidata signals to generate interpretable membership degrees across categories and enable principled multi-category classification. This fusion is particularly valuable for smart tourism applications, as it supports robust faceted exploration and personalized recommendations (e.g., “historic + coastal”), while providing evidence-based explanations that enhance user trust and facilitate curator oversight when POI metadata is sparse or ambiguous. The resulting pipeline produces ranked POI catalogs by country and category, country-level tourism profiles, and diagnostic views for examining uncertain cases. The approach is fully reproducible and readily adaptable to other geographic regions or domain taxonomies. Full article

Historic blue bricks are fundamental to Beijing’s architectural heritage, yet cross-site compositional data for guiding material-compatible restoration remain scarce. This study applies WD-XRF, XRD, SEM, thermal expansion measurement, and physical property testing to 21 blue brick specimens from four Beijing-area sites spanning the Tang through Qing dynasties, with PCA and K-means clustering used to explore compositional grouping structures. Within this exploratory dataset, a compositional distinction separates the Ming and Qing Great Wall bricks: CaO falls from 7.7 to 1.5 wt.% as anorthite gives way to albite, while Qing specimens are denser (1.79 vs. 1.65 g·cm⁻³) with lower water absorption (15.9% vs. 20.9%). Two Wanping City bricks are strongly sulfate-enriched (SO₃ up to 9.8%), and WP-SE3 additionally carries a heavy chloride load (Cl 2.1%), masking their original clay signatures and illustrating how unrecognized weathering can distort compositional grouping and source-related interpretation from bulk chemistry. K-means clustering yields compositional types that overlap only partially with site boundaries, capturing raw material variation rather than site-specific manufacturing fingerprints. Despite constraints in sample size and physical property coverage, the integrated dataset offers preliminary compositional benchmarks and limited performance data to inform period-specific brick replacement at these heritage sites. Full article

The Chishui River Basin, a core production area for Chinese sauce-aroma Baijiu (exemplified by Moutai), supports sorghum cultivation critical to the liquor’s distinctive quality. The soil environment quality within this region, therefore, directly impacts the safety and quality of both raw material and the final distilled spirit. To underpin the safe production and sustainable development of this iconic beverage, it is essential to assess soil heavy metal contamination in the soils and quantify the contributions from various sources. In this study, 172 surface soil samples were collected from typical sorghum planting bases in the Renhuai area. Concentrations of eight heavy metals (loids) (As, Cd, Cr, Cu, Hg, Ni, Pb, and Zn) were determined. The contamination status was evaluated using the geostatistical inverse distance weighting interpolation, the Nemerow pollution index (P_N), and the potential ecological risk index (RI). Source identification and quantification were performed using the positive matrix factorization receptor model (PMF). Results revealed significant enrichment of Cd and Hg in the soil, with mean concentrations 2.07 times and 2.54 times the soil background values for Guizhou Province, respectively. Pollution index results (P_i, P_N) indicated that soil Cd contamination is relatively severe, whereas contamination from other elements is minimal. Overall, approximately 86.5% of the study area was classified as clean or only slightly polluted. Cd poses a moderate ecological risk and was the primary contributor to the total ecological hazard. Other elements exhibited lower risk, resulting in a slight overall potential ecological risk. The soil environmental quality in certified organic sorghum bases was generally favorable. PMF analysis identified three principal sources: historic industrial emissions and traffic-related sources (contributing 46%), weathering of carbonate rocks combined with agricultural activities (37%), and natural background coupled with organic fertilizer application (17%). In conclusion, while the overall soil heavy metal pollution level in the sorghum planting areas is low, the notable enrichment and higher ecological risk of Cd necessitate enhanced dynamic monitoring and targeted risk control measures to ensure long-term soil health and product safety. Full article

Introduction: Skates (Rajidae) are cornerstone elasmobranchs, yet their intrinsic biological constraints, like slow growth, late maturation, and low fecundity, render them exceptionally susceptible to anthropogenic pressure. Despite their ecological and economic importance, tracking their population trajectories is historically hindered by “taxonomic blurring” and aggregated reporting in commercial fisheries. Objective: This study evaluates long-term conservation trends and exploitation dynamics of Rajidae species in the Northeast Atlantic and the Mediterranean Sea. Methodology: We analyzed 31 Rajidae species across the Northeast Atlantic and the Mediterranean Sea (FAO Areas 27 and 37) by integrating IUCN Red List assessments, species-specific life-history traits (maximum body size and depth distribution), and FAO fisheries landing data from 1992 to 2023. Descriptive analyses and Spearman correlations were used to assess temporal trends in conservation status and exploitation patterns. Results: Our synthesis reveals that some species show improvements in IUCN Red List category assessments, likely driven by recent management interventions such as species-specific reporting, catch quotas, and targeted retention bans. However, we also identify a critical mismatch between policy and biology: current Total Allowable Catches (TACs) and minimum landing sizes often do not explicitly incorporate species-specific life-history traits, inadvertently favoring smaller, less-marketable taxa while leaving larger, vulnerable species at risk. While FAO landings offer a valuable broad-scale overview of exploitation, the results highlight the limitations of aggregated fisheries statistics for species-level conservation assessments. Conclusions: These findings underline the need to adopt more precise and species-specific fisheries management approaches for Rajidae, including expanded regional monitoring programs, the use of data collected by on-board observers or electronic monitoring tools, and improved control of data reporting procedures, to prevent continued aggregation of species-level data. Full article

In the context of China’s stock-based urban renewal, public space regeneration in old urban areas increasingly requires attention to everyday use, inclusive access, local memory, and collaborative governance alongside physical upgrading. Drawing on catalyst theory, this study builds an analytical framework linking catalyst classification, potential element identification, effectiveness evaluation, actor collaboration, and renewal strategy transformation. The Guibeicheng area of Wuxi, China, is examined using semi-structured interviews, cognitive maps, qualitative coding, space syntax, the analytic hierarchy process, and actor collaboration analysis. The analysis indicates that behavioral and narrative catalysts are closely associated with residents’ everyday use and place identity. Event catalysts may generate phased amplification effects under specific conditions, while organizational and rule-based governance catalysts mainly provide support conditions for sustaining catalytic effects. Comparing space syntax results with cognitive-map and interview evidence further points to mismatches between configurational potential and perceived everyday activation. These include high-integration spaces with limited evidence of repeated everyday use, high-choice nodes mainly associated with pass-through use, weak everyday connections to historical resources, and limited independent organizational support for high-priority catalysts. On this basis, the study proposes a renewal pathway that combines everyday behavior guidance, event transformation, local narrative embedding, and organizational governance coordination. The findings provide a case-based reference for catalyst-oriented public space regeneration in historic urban areas and suggest potential implications for social sustainability, cultural continuity, and community resilience through spatial activation and long-term collaborative governance. Full article

Quasi-Industrial Districts (QIDs) in Japan allow the coexistence of industrial, residential, and commercial functions. However, under pressures such as deindustrialization, demographic decline, and urban restructuring, their functional balance has been increasingly disrupted. This study investigates the spatiotemporal evolution of QIDs in Kitakyushu and develops a GIS-based framework to quantify changes in land-use structure. Using historical zoning and building floor-area data from 1986 to 2024, ternary diagram analysis is applied to examine relationships among the three functional types and identify transformation trajectories. Results show that while the total QID area expanded by 38.8%, internal structures changed significantly. Industry-dominant districts declined, commerce-oriented districts increased, and residential–industrial mixed types largely disappeared, indicating a shift toward commercial and residential functions. These findings reveal a growing mismatch between zoning designations and actual land use. To address this, the study proposes combining industrial concentration with clearer residential zoning, supported by periodic evaluation based on functional deviation thresholds. The framework provides a quantitative tool for adaptive land-use governance in shrinking industrial cities. Full article