Search Results (5,308)

Recent studies have revealed a specific relationship between gut bacteria and inflammatory skin profiles. We aimed to perform a species-level comparative metagenomic analysis of the gut microbiome in patients with psoriasis, hidradenitis suppurativa (HS), and pemphigus foliaceus (PF). We included omnivorous nonsmokers and nondrinkers with psoriasis (n = 24), HS (n = 10), and PF (n = 11), as well as healthy controls (n = 10). We collected faecal samples from all patients for classic parasitological analysis. Gut microbiome analysis was conducted using shotgun next-generation sequencing. We used the Deseq2, Limma_voom, LinDA, and MaAMaAsLin 2 bioinformatics tools to evaluate concordance and differential abundance between patients. Thirteen patients (23.64%) were diagnosed with active intestinal parasitosis. The presence of intestinal parasitosis was significantly related to immunosuppression (p = 0.009). The most abundant microorganism species found in the faeces of the patients evaluated was Escherichia coli. Psoriasis patients presented a greater abundance of bacteria from the Veillonellaceae family, whereas PF patients presented a greater abundance of Firmicutes bacteria. Patients with PF showed increased E. coli virulence and antibiotic resistance functional markers. Immunosuppression significantly influenced the presence of intestinal parasitosis as well as increased the virulence of functional markers in patients with PF receiving systemic corticosteroid therapy. Full article

Background/Objectives: Post-COVID-19 pulmonary fibrosis (PCPF) and idiopathic pulmonary fibrosis (IPF) exhibit significant clinical and pathophysiological overlap, suggesting convergent molecular pathways driving fibrosis. This prospective longitudinal study investigates the sustained dysregulation of matrix metalloproteinases (MMP)-2 and MMP-9 and its relationship with evolving systemic inflammation and endothelial dysfunction in convalescent COVID-19 patients, with comparative analysis to IPF. Methods: We conducted a prospective observational study of 86 patients at 6 and 12 months post-SARS-CoV-2 infection, stratified by high-resolution CT evidence of PCPF (FB+ group, n = 32) or absence of fibrosis (FB− group, n = 54). Gene expression of MMP-2 and MMP-9 in peripheral blood leukocytes and circulating levels of MMP-2, MMP-9, pro-inflammatory cytokines (TNF-α, IL-6), and endothelial dysfunction markers (Endothelin-1 [ET-1], adhesion molecules) were quantified via qRT-PCR and ELISA. A pre-pandemic healthy control group (HD, n = 20) and an IPF patient group (n = 10) served as comparators. Results: A significant, sustained elevation of MMP-2 and MMP-9 was observed in all post-COVID-19 patients versus HDs, most pronounced in the FB+ group and qualitatively similar to IPF. A critical divergence emerged: FB− patients showed resolution of systemic inflammation (reduced TNF-α, IL-6), whereas FB+ patients exhibited persistent cytokine elevation. Critically, a delayed, severe endothelial dysfunction, characterized by a profound surge in ET-1 and elevated adhesion molecules, manifested exclusively in the FB+ cohort at 12 months. Positive correlations linked plasma MMP-2/9 levels with ET-1 (r_s = 0.65, p = 0.004; r_s = 0.49, p = 0.009) and ET-1 with sICAM-1 (r_s = 0.68, p = 0.01). Conclusions: The development of PCPF is associated with a distinct pathogenic triad: sustained MMP dysregulation, failure to resolve inflammation, and severe late-phase endothelial dysfunction. The correlative links between these components suggest a self-reinforcing loop. This systemic signature mirrors patterns in IPF, underscoring shared final pathways in fibrotic lung disease and identifying the MMP–inflammation–endothelial axis as a promising target for biomarker development and therapeutic intervention. Full article

This paper investigates the knowledge, attitudes, and practical adherence to the Mediterranean Diet (MD) among students of the Teacher Education Study Program in Split. Recent trends indicate a decline in adherence within Mediterranean regions, a phenomenon known as the Mediterranean paradox. Studying the relationship between students’ knowledge and practice is critical within the context of SDG 3 and SDG 4, as it highlights the role of future educators in promoting healthy communities. A quantitative approach was employed using the MDNK methodology, supplemented with the MEDAS test, to assess adherence to the Mediterranean Diet. Statistical analysis included p-values and effect size measures to assess practical relevance. Students averaged 13.39/18 on the MDNK test, with most showing moderate MEDAS adherence and only 5 reaching high adherence. The year of study or employment status has not been shown as an influential factor. While most students possess a high level of knowledge on the MD’s key components and express a positive attitude toward this dietary pattern, a significant knowledge-practice gap exists, confirming the Mediterranean paradox among future teachers. The need for nutritional and food education within the university curriculum is essential to move beyond theoretical instruction and actively promote food literacy and practical skills. Full article

Cognitive reserve (CR) is widely recognized as a protective factor that supports cognitive functioning across the lifespan. Recent research suggests a reciprocal relationship between CR and creative thinking—particularly divergent thinking (DT)—with DT potentially contributing to and benefiting from CR and remaining relatively preserved in older adulthood. This cross-sectional study, conducted in Italy between April and July 2025 using convenience sampling, examined whether CR predicts verbal and conceptual creativity in healthy older adults. One hundred participants (aged 65–92 years; M = 68.45, SD = 8.12) completed the Cognitive Reserve Index questionnaire (CRIq), the Test di Intelligenza Breve (TIB; Short Intelligence Test), and two creativity tasks. Data were analyzed using IBM SPSS Statistics (version 25.0; IBM Corp., Armonk, NY, USA). Multiple regression analyses showed that overall CR significantly predicted all creativity outcomes, including verbal fluency (β = 0.316, p = 0.011) and flexibility (β = 0.336, p = 0.007), as well as conceptual fluency (β = 0.371, p = 0.003), flexibility (β = 0.381, p = 0.002), and originality (β = 0.338, p = 0.006). Education and leisure activities more strongly predicted verbal creativity, whereas occupational experience and leisure activities predominantly predicted conceptual creativity. These findings indicate that CR supports creative thinking in later life and highlight the importance of cognitively and socially enriched experiences across the lifespan. Full article

Background: Serum C–C motif chemokine ligand 18 (seCCL18) in systemic sclerosis (SSc) has been primarily associated with progressive interstitial lung disease (SSc-ILD) and mortality. However, its relationship with non-pulmonary organ involvement, disease activity, and long-term outcome has not been comprehensively evaluated. We therefore examined the clinical relevance of seCCL18 in a single-center SSc cohort. Methods: A total of 151 patients with SSc (83 diffuse cutaneous (dcSSc), 68 limited cutaneous SSc (lcSSc); median (IQR) disease duration: 9 (4;16) years) and 47 age- and sex-matched healthy controls (HCs) were enrolled. Serum CCL18 concentrations were measured by enzyme-linked immunosorbent assay. Elevated seCCL18 was defined as >130 ng/mL (mean + 2 SD of the healthy control group). Organ involvement and disease activity (EUSTAR Activity Index, EUSTAR-AI) were assessed at baseline, while survival was analysed longitudinally. Results: Patients with SSc had significantly higher seCCL18 levels than HCs (mean ± SD: 99.9 ± 43.2 vs. 75.0 ± 27.5 ng/mL, p < 0.01). Elevated seCCL18 was associated with SSc-ILD (81.1% vs. 60.5%, p = 0.022), reduced forced vital capacity (FVC < 70%: 16.2% vs. 3.5%, p = 0.006), and reduced diffusing capacity for carbon monoxide (DLCO < 70%: 80.6% vs. 54.4%, p = 0.005). Higher seCCL18 levels were observed in patients with myocardial disease (104.8 ± 41.8 vs. 83.8 ± 44.2 ng/mL, p = 0.008), left ventricular diastolic dysfunction (107.1 ± 40.5 vs. 84.5 ± 45.0 ng/mL, p < 0.001), and oesophageal involvement (110.7 ± 38.3 vs. 93.3 ± 43.1 ng/mL, p = 0.009). SeCCL18 levels above the cut-off were more frequently associated with tendon friction rubs (51.4% vs. 27.4%, p = 0.007), active disease (EUSTAR-AI ≥ 2.5: 73% vs. 44%, p = 0.002), and elevated inflammatory markers (CRP > 5 mg/L: 51.4% vs. 19.3%, p < 0.001; ESR > 28 mm/h: 37.8% vs. 18.4%, p = 0.015). During a median follow-up of 87 months, 22 patients (15%) died. Elevated baseline seCCL18 predicted poorer survival in univariate analysis (log-rank p = 0.013) and remained an independent predictor of mortality in multivariable Cox regression (HR 1.789; 95% CI 1.133–2.824; p = 0.013), together with declining DLCO and reduced six-minute walk test performance. Conclusions: Elevated seCCL18 may identify patients with systemic sclerosis who exhibit a more severe multisystem phenotype, including cardiopulmonary, gastrointestinal, and musculoskeletal involvement, increased inflammatory activity, and reduced long-term survival. These findings suggest that seCCL18 may have some clinical utility as a prognostic biomarker reflecting widespread disease involvement beyond the lungs, even in patients with long-standing disease; however, the lack of an established cut-off value requires further validation in prospective, multicentre studies. Full article

Social–emotional development in early childhood lays the groundwork for school readiness, healthy relationships, and long-term well-being. Parents play a pivotal role in this process, shaping children’s emotional awareness, regulation, and social competence through everyday interactions. This literature review synthesizes evidence from 74 peer-reviewed studies to evaluate nine evidence-based parenting interventions targeting parents of preschool-aged children. The programs were analyzed with respect to their objectives, theoretical foundations, components, and the resulting outcomes for both parents and children. Across interventions, consistent benefits emerged in children, including improved emotion recognition, regulation, empathy, and prosocial behavior, as well as reductions in internalizing problems. Parents also gained in confidence and positive discipline practices. Key elements linked to effectiveness included active parent skill-building (such as modeling, role play, and guided practice), structured parent–child interactions, multi-component designs integrating home and school contexts, and flexible delivery formats that adapt to family needs. These findings underscore the critical role of parenting interventions as an evidence-based method to enhance preschoolers’ social–emotional development. Full article

The healthy evolution of an entrepreneurial ecosystem relies on the symbiotic relationships among its diverse internal actors. This study addresses a gap in entrepreneurial ecosystem research, which has predominantly focused on two-agent models, by constructing a tripartite symbiotic evolution model that incorporates entrepreneurial ventures, incubation chains, and customers. Based on the Logistic and Lotka-Volterra models, the research identifies the system’s equilibrium points and their stability conditions. Simulations reveal evolutionary paths from parasitism and commensalism to mutualism. A comparative case study of SenseTime (Shanghai, China) and Lanma Technology (Shanghai, China) validates these findings. The comparison shows that an influx of multiple agents, coupled with the core venture’s ability to strengthen key symbiotic coefficients, drives the ecosystem towards a dynamic multi-agent symbiosis in the post-optimization phase. Conversely, the failure to establish these robust reciprocal value flows leads to ecosystem fragility. The results indicate that: (1) Multi-agent entrepreneurial ecosystems are complex systems where symbiotic units form adaptive relationships for value creation, adhering to market laws. (2) The system’s equilibrium depends on symbiotic coefficients, leading to four modes—independent coexistence, parasitism, commensalism, and mutualism—with mutualism being the optimal state. (3) The contrasting cases further demonstrate that the evolution towards mutualism is not automatic but hinges on the core venture’s strategic agency in constructing and strengthening synergistic pathways with forward and backward linkages. This study provides a theoretical model for understanding the evolutionary mechanisms of entrepreneurial ecosystems and offers practical insights for optimizing ecosystem governance. Full article

This work investigates the mechanisms of information transfer underlying causal relationships between brain regions during resting-state conditions in patients with schizophrenia (SCZ). A large fMRI dataset including healthy controls and SCZ patients was analyzed to estimate directed information flow using local Transfer Entropy (TE). Four functional interaction patterns—referred to as rules—were identified between brain regions: activation in the same state (ActS), activation in the opposite state (ActO), turn-off in the same state (TfS), and turn-off in the opposite state (TfO), indicating a dynamics toward converging (Acts/Tfs = S) and diverging (ActO/TfO = O) states of brain regions. These interactions were integrated within a multiplex network framework, in which each rule was represented as a directed network layer. Our results reveal widespread alterations in the functional architecture of SCZ brain networks, particularly affecting schizophrenia-related systems such as bottom-up sensory pathways and associative cortical dynamics. An imbalance between S and O rules was observed, leading to reduced network stability. This shift results in a more randomized functional network organization. These findings provide a mechanistic link between excitation/inhibition (E/I) imbalance and mesoscopic network dysconnectivity, in agreement with previous dynamic functional connectivity and Dynamic Causal Modeling (DCM) studies. Overall, our approach offers an integrated framework for characterizing directed brain communication patterns and psychiatric phenotypes. Future work will focus on systematic comparisons with DCM and other functional connectivity methods. Full article

Bilateral morphological asymmetry of the upper limbs may influence grip strength even in semi-active young adults. Understanding this relationship is important for identifying early neuromuscular imbalances with implications for ergonomics and rehabilitation. This study aimed to examine associations between upper limb anthropometric characteristics and grip strength in non-athlete students, considering gender and manual dominance. The sample included 192 healthy university students (110 females, 82 males; mean age 19.92 ± 1.4 years) without prior sports training. Thirteen bilateral anthropometric parameters of the upper limbs were assessed, including hand and palm dimensions, segmental lengths, and arm and forearm circumferences, along with grip strength measured by dynamometry in two positions: arm extended and arm flexed at 90°. Statistical analysis revealed significant differences in forearm length, arm and forearm circumferences, and grip strength (p < 0.001). The dominant limb consistently demonstrated higher grip strength, with mean differences of approximately 2 kg. Male participants showed higher absolute values for all morphological and functional variables, whereas stronger correlations between distal upper-limb morphology and grip strength were observed in females. These findings indicate that, despite largely symmetric skeletal dimensions, moderate functional asymmetries exist and grip strength is influenced primarily by local muscular development rather than overall limb size. Full article

Background/Objectives: Equol, a gut microbial metabolite of the soy isoflavone, daidzein, is associated with estrogenic activity and potential benefits for women’s health. While equol production depends on individual gut microbial composition, its dietary and clinical correlates in young women remain incompletely characterized. This study explored the relationship between urinary equol production, dietary habits, and premenstrual symptom severity in healthy university-aged women. Methods: We conducted a cross-sectional study of 41 Japanese women, aged 19–20 years. Urinary equol was measured using a validated liquid chromatography–tandem mass spectrometry (LC–MS/MS) method, following enzymatic hydrolysis. Participants were classified as either equol producers or non-producers, based on urinary concentration thresholds. Dietary intake was evaluated using a dietary questionnaire focused on soy products and dietary fiber sources. Premenstrual symptoms were assessed using a standardized Japanese questionnaire for premenstrual syndrome and premenstrual dysphoric disorder. Results: Twelve percent of participants were classified as equol producers. Compared with non-producers, equol producers reported higher consumption of pumpkin, soybean sprouts, and green tea. Among non-producers, higher consumption of certain vegetables and fiber-rich foods, including broccoli, pickled radish, konjac, and konjac jelly, was associated with greater premenstrual symptom severity, whereas such associations were not observed among equol producers. The analytical method demonstrated high sensitivity and reproducibility for urinary equol measurement. Conclusions: These findings suggest that equol production status may be associated with distinct dietary patterns and with differences in the relationship between food intake and premenstrual symptom severity in young women. Although the cross-sectional design and limited sample size preclude causal inference, these findings suggest that urinary equol is a promising candidate biomarker for future research on diet-related modulation of premenstrual symptoms. Full article

Background and Objectives: There is a lack of information on the dietary intake of long-term combined oral contraceptive users (COC-users) in relation to their body composition. The aim of the pilot study was to determine the food consumption of young women using COCs for >6 months and its relationship to body composition compared to non-users. Materials and Methods: A total of 45 healthy women (21.8 ± 1.7 years) were divided into COC non-users (n = 19) and users of a low (≤20 μg) ethinylestradiol (EE) dose (n = 18) and a medium (30–35 μg) EE dose (n = 8). Anthropometric data, body composition, physical activity levels, three-consecutive-days of dietary records, and serum concentrations of calcium, magnesium, and vitamin D were assessed. Statistical methods included chi-squared, Fisher’s exact test, t-test, Mann–Whitney U test, Spearman’s correlation, and multiple linear regression. Results: There were no differences between COC-users and non-users in terms of physical activity, intake of micronutrients, body mass index, body fat percentage (BF%), or bone mineral density (BMD). COC-users consumed a higher percentage of energy from fats (p = 0.02) and had higher serum vitamin D levels (p < 0.01). BF% was negatively associated with BMD (coefficient −0.008; p = 0.027). Conclusions: The use of COCs for ˃6 months is not associated with FM or BMD, regardless of dietary differences. Further attention should be paid to possible associations between COCs use, dietary habits, and body composition (including BMD). Full article

Objective: This study investigates the association between consumers’ perceived readability of Multiple Traffic Light (MTL) label print size—a theoretical structural gatekeeper for visual salience—and self-reported food consumption frequency in the United Kingdom. We aimed to disentangle the effect of label readability from label content. Using non-equivalent dependent variables (NEDVs), we tested whether the association is specific to unhealthy convenience foods and absent for healthy or unlabeled foods, while also examining heterogeneity across consumer subgroups. Methods: Data from 8948 adults across four waves (2012–2018) of the UK Food and You Survey were analyzed. Cumulative link ordinal logistic regressions were employed to model the association between self-reported print size readability and the consumption frequency of four product types: pre-packaged sandwiches and pre-cooked meat (unhealthy, labeled targets), dairy (nutritionally advisable, labeled control), and fresh meat (unlabeled control). Models were adjusted for sociodemographic covariates, health behaviors, and survey wave fixed effects. Results: The findings reveal a content-specific and significant dynamic relationship exclusively for pre-packaged sandwiches. In 2012, a one-unit increase in readability was associated with a 9% decrease in the odds of frequent consumption ($OR=0.91$), consistent with a warning effect. However, by 2018, this relationship reversed to a 4% increase ($OR=1.04$), indicating that higher readability became associated with more frequent consumption. In contrast, a persistent null association was observed for pre-cooked meat, dairy, and fresh meat. Subgroup analyses for sandwiches indicated that the association with readability was strongest among less-engaged consumers. Conclusions: Empirical evidence challenges the utility of a standardized approach to food labelling. The results suggest that the effectiveness of label salience is contingent not just on the consumer but on the product’s context and the content of its message, highlighting the need for adaptive rather than uniform policy standards. Full article

The growth and development of breast cancer are accompanied by an increase in oxidative stress. A close relationship is known to exist between the biological activity of several antioxidant enzymes and the regulation of estrogen-mediated signaling in breast cancer. The aim of this study was to study the activity of salivary antioxidant enzymes and the level of lipid peroxidation products in breast cancer before and after surgical treatment. The study included 115 patients with breast cancer (58.7 ± 10.9 years) and 60 healthy volunteers (51.8 ± 12.1 years). Saliva samples were obtained again from 53 patients 4 weeks after surgery. The content of lipid peroxidation products, catalase activity, total antioxidant activity (AOA) and total peroxidase activity (TPA) in saliva were analyzed before and after breast cancer surgery. An increase in lipid peroxidation products in saliva was observed with positive estrogen receptor expression. For the first time, it was shown that in patients with breast cancer, the levels of salivary TPA and AOA increased, which is likely due to the important role of the salivary glands in antioxidant protection. It can be speculated that the effectiveness of antioxidant defense was associated with estrogen and progesterone receptor expression and was reduced in prognostically unfavorable breast cancer phenotypes (non-luminal and triple-negative breast cancer). Full article

Background: Identifying factors associated with cognitive impairment among older adults is critical. This study investigates both concurrent and longitudinal associations between sleep quality, sleep duration, and cognitive performance among older adults in China, with particular emphasis on the moderating role of fruit and/or vegetable intake (FVI), a factor rarely examined in previous research. Methods: We pooled five waves of a specially designed nationwide sample of adults aged 65 years or older (N = 64,690; mean age: 86.3 years; men: 43.5%) in 2005, 2008, 2011, 2014, and 2018 in China. Cognitive impairment was assessed by the Mini-Mental State Examination. Among the sample, 10.7% were cognitively impaired. FVI was dichotomized into frequent (almost daily) versus infrequent (other low frequencies). Sleep hours were grouped into short (≤6 h), normal (7–9 h), and long (≥10 h) durations. Both concurrent and cross-lagged analyses were performed after adjusting for a wide set of covariates (demographics, socioeconomic status, family/social connections, health practices, disability, self-rated health, and chronic conditions). Analyses were further stratified by gender, age group, and urban–rural residence. Results: When all covariates were present (the full model), good sleep quality was associated with 22% lower odds of the prevalence of cognitive impairment, whereas the long sleep duration was associated with 24% higher odds as compared with the normal sleep duration. Although the short sleep duration was not associated with the prevalence of cognitive impairment in the full model, it was associated with 8% higher odds of cognitive impairment when health condition was not controlled for. Interaction analyses revealed that frequent FVI buffered the adverse cognitive effects of poor sleep quality and both short and long sleep durations. Subgroup analyses further show similar patterns across subpopulations, with more pronounced protective associations in older women and the oldest-old. Conclusions: Good sleep quality, normal sleep durations, and frequent FVI jointly contribute to better cognitive functioning at older ages. While the observed relationships are largely concurrent rather than causal, promoting both healthy sleep and dietary habits may be important for cognitive health among older adults. Full article

Background: The COVID-19 virus is a source of both acute and chronic stress for many people. This stress could uniquely impact children and their mental health. Research has shown that children with neurodevelopmental disorders such as Attention-Deficit/Hyperactivity Disorder (ADHD) are at an increased risk of negative mental health symptoms due to stress, but high-quality sleep may be associated with a protective role against these symptoms. We, therefore, aimed to investigate whether the impacts of COVID-19 and sleep problems were independently linked with children’s mental health and to examine whether sleep could mediate the relationship between COVID-19 impact and child mental health. Finally, we sought to compare the degree to which sleep problems could mediate this relationship in children without ADHD and in children with ADHD. Methods: In this cross-sectional study, a total of 304 parents of children were sampled from a larger study investigating the impact of the COVID-19 pandemic on Canadian families and children in the spring of 2021. Parents reported on their children’s mental health, sleep, and the impacts of COVID-19 on their child. Of the total sample, 234 children were reported as having an ADHD diagnosis, and 70 children were reported to not have ADHD. Results: We found that both the impact of COVID-19 and sleep problems independently and positively contributed to the mental health symptoms (p < 0.001) experienced by children with ADHD and without ADHD. Children with ADHD were found to have higher scores for COVID-19 child impact, sleep problems, and negative mental health. However, sleep problems had a greater impact on the mental health of children without ADHD compared to ADHD children. Additionally, the results suggest that sleep problems mediated ~20% of the relationship between COVID-19 impact and child mental health in children with ADHD and ~51% of this relationship in children without ADHD. Conclusions: The findings emphasize the significant role of sleep in mediating child mental health symptoms during periods of stress in children without ADHD and in children with ADHD. We highlight the importance of considering sleep quality and supporting healthy sleep in times of stress to improve child mental health symptoms. Full article