Search Results (18)

Piperazic acid is a cyclic nonproteinogenic amino acid that contains a hydrazine N-N bond formed by a piperazate synthase (KtzT-like). This amino acid, found in bioactive natural products synthesized by non-ribosomal peptide synthetases (NRPSs), confers conformational constraint to peptides, an important feature for their biological activities. Genome mining of Streptomyces strains has been revealed as a strategy to identify biosynthetic gene clusters (BGCs) for potentially active compounds. Moreover, the isolation of new strains from underexplored habitats or associated with other organisms has allowed to uncover new BGCs for unknown compounds. The in-house “Carlos Sialer (CS)” strain collection consists of seventy-one Streptomyces strains isolated from the cuticle of leaf-cutting ants of the tribe Attini. Genomes from twelve of these strains have been sequenced and mined using bioinformatics tools, highlighting their potential to encode secondary metabolites. In this work, we have screened in silico those genomes, using KtzT as a hook to identify BGCs encoding piperazic acid-containing compounds. This resulted in uncovering the new BGC dpn in Streptomyces sp. CS113, which encodes the biosynthesis of the hybrid polyketide–depsipeptide diperamycin. Analysis of the diperamycin polyketide synthase (PKS) and NRPS reveals their functional similarity to those from the aurantimycin A biosynthetic pathway. Experimental proof linking the dpn BGC to its encoded compound was achieved by determining the growth conditions for the expression of the cluster and by inactivating the NRPS encoding gene dpnS2 and the piperazate synthase gene dpnZ. The identity of diperamycin was confirmed by High-Resolution Mass Spectrometry (HRMS) and Nuclear Magnetic Resonance (NMR) and by analysis of the domain composition of modules from the DpnP PKS and DpnS NRPS. The identification of the dpn BGC expands the number of BGCs that have been confirmed to encode the relatively scarcely represented BGCs for depsipeptides of the azinothricin family of compounds and will facilitate the generation of new-to-nature analogues by combinatorial biosynthesis. Full article

In recent years, owing to intense urbanization and global change with the consequent extreme climate effects, interest in green roofs, even extensive ones, in the Mediterranean environment has increased. To this end, the choice of plant species is crucial because, owing to the identification of the most suitable plants, it will be possible to expand this type of green infrastructure and increase its ecosystem services in the urban environment. In this context, the objective of the review, through a critical analysis of some of the references on the topic, is to identify suitable criteria for plant species selection that are simple to apply and able to respond to the need to have plants capable of surviving, ensuring a suitable aesthetic effect, and providing essential ecosystem services. We also investigated whether, and to what extent, associations of different species can better adapt to the difficult environmental conditions of Mediterranean green roofs. Two possible strategies to identify the plant idiotype were analyzed: the analysis of plants present in habitat analogues or the identification of morpho-functional characters capable of discriminating the response to abiotic stress, and in particular to drought stress. The use of plant communities, rather than a single species, seems capable of improving aesthetic effects, plant survival, and ecosystem services. Full article

The active development of the problems related to the assessment of the role of the pedosphere in global climate change involves the possibility of application of the quantitative determination of soil organic carbon (SOC) as one of the indicators of a climatic response. Here, the authors have summarized the results of their own research over many years (1985–2023), comprising more than 500 determinations of SOC within the area of the Chernozem zone, in the south of the East European Plain (Moldova and Bessarabia, southern Ukraine, southwestern Russia), in the context of regional climate differentiation using evaluations of climatic energy consumption for soil formation. The data were structured for each of the regions through the creation of series of agrogenic soil transformations (virgin land, modern-day ploughed land (<100 years), continually ploughed land (>100 years), fallow land of the modern era (n·10 years), and post-antique long-term fallow land). It has been established, by means of statistical treatment of the data, that the intraregional differentiation of the bioclimatic conditions is the key factor determining the SOC content in the top horizon of soils in the south of the East European Plain. The comparison of the SOC content within the five variants of land use demonstrated that all the regions under study differed, with statistical significance only found in a single type of ‘continually ploughed land’ (>100 years). This fact reflects the leading role of the duration of agrarian loads in agropedogenesis. If the steppe Chernozems even 145 years ago had a SOC content of up to 4%, then the Chernozems in the forest-steppe zone, which used to have habitats with a SOC content of 4–7%, occupied the largest areas, and have now lost 30–40% of the original values in the 0–50 cm layer. Besides the rates of the SOC degradation, which are known and are comprehensively evaluated in the present work, the phenomenon of progradation was established in certain situations, in particular arising during the rotation regime of land use (from ploughing to fallow fields, and vice versa), which stimulated effective mechanisms of reproduction of organic substances. Thus, in one of the ancient agricultural regions, where in antiquity the land was cultivated by such ancient Greek states as Tauric Chersonesos and the European Bosporos in Crimea, post-antique long-term fallow lands possess higher SOC contents than their virgin analogues. It is not justified to consider virgin lands as absolute references for the evaluation of the humus conditions since the analysis of agrogenic series of Chernozems has corroborated an essential role of the soil organo-mineral matrix in the formation of the carbon protection capacity. Full article

Legumes and nuts are components of high importance in the diet of many countries, mainly those in the Mediterranean region. They are also very versatile and culturally diverse foods found all over the world, acting as a basic protein source in certain countries. Their genetic diversity is needed to sustain the food supply and security for humans and livestock, especially because of the current loss of habitats, species, and genetic diversity worldwide, but also because of the ever present need to feed the increasing human population. Even though both legumes and nuts are considered as high-protein food and environmentally friendly crops, developed countries have lower consumption rates when compared to Asia or Africa. With a view to increasing the consumption of legumes and nuts, the objective of this review is to present the advantages on the use of autochthonous varieties from different countries around the world, thus providing a boost to the local market in the area. The consumption of these varieties could be helped by their use in ready-to-eat foods (RTE), which are now on the rise thanks to today’s fast-paced lifestyles and the search for more nutritious and sustainable foods. The versatility of legumes and nuts covers a wide range of possibilities through their use in plant-based dairy analogues, providing alternative-protein and maximal amounts of nutrients and bioactive compounds, potential plant-based flours for bakery and pasta, and added-value traditional RTE meals. For this reason, information about legume and nut nutrition could possibly increase its acceptance with consumers. Full article

Edible mushrooms are widely appreciated for their appealing flavours, low caloric values and high content of presumably health-protecting metabolites. Their long history of safe use together with the looming worldwide food crisis have revived the idea of generating meat analogues and protein isolates by the controlled fermentation of mycelia of these edible fungi as a dietary option. The occurrence of proteins, polysaccharides, smaller metabolites, metal ions and toxins in mycelia and fruiting bodies is compared among the three most popular species, Agaricus bisporus (button mushroom), Pleurotus ostreatus (oyster mushroom), Lentinus edodes (shiitake) and some closely related species. Large effects of substrate chemistry, strain, developmental stage and ecological interactions result in a wide variation of the concentrations of some metabolites in both mycelial cells and fruiting bodies. This is obviously a result of the high adaptation abilities required to survive in natural habitats. Fungal bioprocesses are decoupled from agricultural production and can be operated anytime, anywhere, and on any scale according to demand. It is concluded that fungal biomass, if produced under food-grade conditions and on an industrial scale, could provide a safe and nutritious meat substitute and protein isolates with a high biological value for future vegan foods. Full article

The populations, species, and communities in high elevation mountainous regions at or above tree line are being impacted by the changing climate. Mountain systems have been recognized as both resilient and extremely threatened by climate change, requiring a more nuanced understanding of potential trajectories of the biotic communities. For high elevation systems in particular, we need to consider how the interactions among climate drivers and topography currently structure the diversity, species composition, and life-history strategies of these communities. Further, predicting biotic responses to changing climate requires knowledge of intra- and inter-specific climate associations within the context of topographically heterogenous landscapes. Changes in temperature, snow, and rain characteristics at regional scales are amplified or attenuated by slope, aspect, and wind patterns occurring at local scales that are often under a hectare or even a meter in extent. Community assemblages are structured by the soil moisture and growing season duration at these local sites, and directional climate change has the potential to alter these two drivers together, independently, or in opposition to one another due to local, intervening variables. Changes threaten species whose water and growing season duration requirements are locally extirpated or species who may be outcompeted by nearby faster-growing, warmer/drier adapted species. However, barring non-analogue climate conditions, species may also be able to more easily track required resource regimes in topographically heterogenous landscapes. New species arrivals composed of competitors, predators and pathogens can further mediate the direct impacts of the changing climate. Plants are moving uphill, demonstrating primary succession with the emergence of new habitats from snow and rock, but these shifts are constrained over the short term by soil limitations and microbes and ultimately by the lack of colonizable terrestrial surfaces. Meanwhile, both subalpine herbaceous and woody species pose threats to more cold-adapted species. Overall, the multiple interacting direct and indirect effects of the changing climate on high elevation systems may lead to multiple potential trajectories for these systems. Full article

Terrestrial plants evolution occurred in the presence of microbes, the phytomicrobiome. The rhizosphere microbial community is the most abundant and diverse subset of the phytomicrobiome and can include both beneficial and parasitic/pathogenic microbes. Prokaryotes of the phytomicrobiome have evolved relationships with plants that range from non-dependent interactions to dependent endosymbionts. The most extreme endosymbiotic examples are the chloroplasts and mitochondria, which have become organelles and integral parts of the plant, leading to some similarity in DNA sequence between plant tissues and cyanobacteria, the prokaryotic symbiont of ancestral plants. Microbes were associated with the precursors of land plants, green algae, and helped algae transition from aquatic to terrestrial environments. In the terrestrial setting the phytomicrobiome contributes to plant growth and development by (1) establishing symbiotic relationships between plant growth-promoting microbes, including rhizobacteria and mycorrhizal fungi, (2) conferring biotic stress resistance by producing antibiotic compounds, and (3) secreting microbe-to-plant signal compounds, such as phytohormones or their analogues, that regulate aspects of plant physiology, including stress resistance. As plants have evolved, they recruited microbes to assist in the adaptation to available growing environments. Microbes serve themselves by promoting plant growth, which in turn provides microbes with nutrition (root exudates, a source of reduced carbon) and a desirable habitat (the rhizosphere or within plant tissues). The outcome of this coevolution is the diverse and metabolically rich microbial community that now exists in the rhizosphere of terrestrial plants. The holobiont, the unit made up of the phytomicrobiome and the plant host, results from this wide range of coevolved relationships. We are just beginning to appreciate the many ways in which this complex and subtle coevolution acts in agricultural systems. Full article

The history of mycological research and current activities in the Polish part of the Białowieża Primeval Forest are presented. The review of literature-derived and unpublished data on species of non-lichenized fungi and protozoan and chromistan fungal analogues indicates a minimum of 3504 species observed in this area. The gaps in the exploration of fungi: unstudied taxa, plant communities, habitats, hosts, and substrates, as well as the limitations of former studies, are discussed. Our estimates show that a total of 8000 fungal species possibly occur in the Białowieża National Park alone, and more than 10,000 are expected to be found in the Polish part of the Białowieża Primeval Forest. Despite more than a centennial history of mycological research, the majority of data come from only a few older scientific projects and several more recent citizen-science-related activities, emphasizing the need for a modern, interdisciplinary study on the diversity and ecology of fungi in this area. Full article

Beaver dam analogues (BDAs) are becoming an increasingly popular stream restoration technique. One ecological function BDAs might help restore is suitable habitat conditions for fish in streams where loss of beaver dams and channel incision has led to their decline. A critical physical characteristic for fish is stream temperature. We examined the thermal regime of a spring-fed Canadian Rocky Mountain stream in relation to different numbers of BDAs installed in series over three study periods (April–October; 2017–2019). While all BDA configurations significantly influenced stream and pond temperatures, single- and double-configuration BDAs incrementally increased stream temperatures. Single and double configuration BDAs warmed the downstream waters of mean maxima of 9.9, 9.3 °C by respective mean maxima of 0.9 and 1.0 °C. Higher pond and stream temperatures occurred when ponding and discharge decreased, and vice versa. In 2019, variation in stream temperature below double-configuration BDAs was lower than the single-configuration BDA. The triple-configuration BDA, in contrast, cooled the stream, although the mean maximum stream temperature was the highest below these structures. Ponding upstream of BDAs increased discharge and resulted in cooling of the stream. Rainfall events sharply and transiently reduced stream temperatures, leading to a three-way interaction between BDA configuration, rainfall and stream discharge as factors co-influencing the stream temperature regime. Our results have implications for optimal growth of regionally important and threatened bull and cutthroat trout fish species. Full article

The Moon’s environmental conditions present limited opportunities for waste heat dissipation, so internal heat gains (IHG) are a key component of thermal balance in a lunar building. Despite the significant development in energy saving and energy storage technologies of the last thirty years, the issue of IHG in lunar buildings has not been readdressed since the early 1990s. This study is based on an inspection of internal heat sources conducted aboard LUNARES, the first European extraterrestrial analogue habitat. The equipment absent on LUNARES, but indispensable for an actual lunar base, was identified and accounted for, along with additional laboratory and maintenance equipment. Three main groups of internal heat sources were identified and studied in detail. Waste heat generated by electric devices was accounted for, along with occupational heat loads adjusted for lunar partial gravity conditions. Assuming a photovoltaic power source for the studied building, two alternative energy storage systems (ESS) were analysed as another source of waste heat. Depending on the time of lunar day and applied ESS, the nominal IHG were between 73 and 133 W/m². The most significant internal heat sources in a lunar base are life support systems and potentially, regenerative fuel cells; thus, lithium–ion batteries were recommended for ESS. Within assumed parameter range, parametric study exhibited differences in IHG between 41.5 and 163 W/m². Full article

The prokaryotic filamentous marine cyanobacteria are photosynthetic microbes that are found in diverse marine habitats, ranging from epiphytic to endolithic communities. Their successful colonization in nature is largely attributed to genetic diversity as well as the production of ecologically important natural products. These cyanobacterial natural products are also a source of potential drug leads for the development of therapeutic agents used in the treatment of diseases, such as cancer, parasitic infections and inflammation. Major sources of these biomedically important natural compounds are found predominately from marine cyanobacterial orders Oscillatoriales, Nostocales, Chroococcales and Synechococcales. Moreover, technological advances in genomic and metabolomics approaches, such as mass spectrometry and NMR spectroscopy, revealed that marine cyanobacteria are a treasure trove of structurally unique natural products. The high potency of a number of natural products are due to their specific interference with validated drug targets, such as proteasomes, proteases, histone deacetylases, microtubules, actin filaments and membrane receptors/channels. In this review, the chemistry and biology of selected potent cyanobacterial compounds as well as their synthetic analogues are presented based on their molecular targets. These molecules are discussed to reflect current research trends in drug discovery from marine cyanobacterial natural products. Full article

Necessitated by the subzero temperatures and seasonal exposure to ice, various organisms have developed a remarkably effective means to survive the harsh climate of their natural habitats. Their ice-binding (glyco)proteins keep the nucleation and growth of ice crystals in check by recognizing and binding to specific ice crystal faces, which arrests further ice growth and inhibits ice recrystallization (IRI). Inspired by the success of this adaptive strategy, various approaches have been proposed over the past decades to engineer materials that harness these cryoprotective features. In this review we discuss the prospects and challenges associated with these advances focusing in particular on peptidic antifreeze materials both identical and akin to natural ice-binding proteins (IBPs). We address the latest advances in their design, synthesis, characterization and application in preservation of biologics and foods. Particular attention is devoted to insights in structure-activity relations culminating in the synthesis of de novo peptide analogues. These are sequences that resemble but are not identical to naturally occurring IBPs. We also draw attention to impactful developments in solid-phase peptide synthesis and ‘greener’ synthesis routes, which may aid to overcome one of the major bottlenecks in the translation of this technology: unavailability of large quantities of low-cost antifreeze materials with excellent IRI activity at (sub)micromolar concentrations. Full article

Secondary succession is considered a threat to non-forest Natura 2000 habitats. Currently available data and techniques such as airborne laser scanning (ALS) data processing can be used to study this process. Thanks to these techniques, information about the spatial extent and the height of research objects—trees and shrubs—can be obtained. However, only archival aerial photographs can be used to conduct analyses of the stage of succession process that took place in the 1960s or 1970s. On their basis, the extent of trees and shrubs can be determined using photointerpretation, but height information requires stereoscopic measurements. State-of-the-art dense image matching (DIM) algorithms provide the ability to automate this process and create digital surface models (DSMs) that are much more detailed than ones obtained using image matching techniques developed a dozen years ago. This research was part of the HabitARS project on the Ostoja Olsztyńsko-Mirowska Natura 2000 protected site (PLH240015). The source data included archival aerial photographs (analogue and digital) acquired from various phenological periods from 1971–2015, ALS data from 2016, and data from botanical campaigns. First, using the DIM algorithms, point clouds were generated and converted to DSMs. Heights interpolated from the DSMs were compared with stereoscopic measurements (1971–2012) and ALS data (2016). Then, the effectiveness of tree and shrub detection was analysed, considering the relationship between the date and the parameters of aerial images acquisition and DIM effects. The results showed that DIM can be used successfully in tree and shrub detection and monitoring, but the source images must meet certain conditions related to their quality. Based on the extensive material analysed, the detection of small trees and shrubs in aerial photographs must have a scale greater than 1:13,000 or a 25 cm GSD (Ground Sample Distance) at most, an image acquisition date from June–September (the period of full foliage in Poland), and good radiometric quality. Full article

Degradation of streams and associated riparian habitat across the Missouri River Headwaters Basin has motivated several stream restoration projects across the watershed. Many of these projects install a series of beaver dam analogues (BDAs) to aggrade incised streams, elevate local water tables, and create natural surface water storage by reconnecting streams with their floodplains. Satellite imagery can provide a spatially continuous mechanism to monitor the effects of these in-stream structures on stream surface area. However, remote sensing-based approaches to map narrow (e.g., <5 m wide) linear features such as streams have been under-developed relative to efforts to map other types of aquatic systems, such as wetlands or lakes. We mapped pre- and post-restoration (one to three years post-restoration) stream surface area and riparian greenness at four stream restoration sites using Worldview-2 and 3 images as well as a QuickBird-2 image. We found that panchromatic brightness and eCognition-based outputs (0.5 m resolution) provided high-accuracy maps of stream surface area (overall accuracy ranged from 91% to 99%) for streams as narrow as 1.5 m wide. Using image pairs, we were able to document increases in stream surface area immediately upstream of BDAs as well as increases in stream surface area along the restoration reach at Robb Creek, Alkali Creek and Long Creek (South). Although Long Creek (North) did not show a net increase in stream surface area along the restoration reach, we did observe an increase in riparian greenness, suggesting increased water retention adjacent to the stream. As high-resolution imagery becomes more widely collected and available, improvements in our ability to provide spatially continuous monitoring of stream systems can effectively complement more traditional field-based and gage-based datasets to inform watershed management. Full article

Unfortunately, we live in a greedy little world and horizontal areas are often too expensive to leave to nature on them. Therefore, creating gardens on vertical surfaces of urban areas has recently been very important to supporting sustainability because these surfaces are still found to be commercially useless compared with green areas which are generally under pressure from commercial demands and politics. However, these artificial vertical green surfaces are still too far from being common, while too many ordinary walls are spontaneously covered with vegetation already. In this study, we try to address the dynamics of wall vegetation as it has a great potential to make the cities more sustainable. Totally 70 walls (35 in urban and 35 in sub-urban areas) in Trabzon city were examined regarding their ecological, physical and vegetation characteristics. Having identified 1540 plant samples collected from the walls during a year-round intensive field study, we performed statistical analyzes to enumerate the wall vegetation depending on the ecological characteristics; to evaluate if there are different wall vegetation compositions in urban and sub-urban areas; and finally to evaluate growth conditions and basic challenges for the wall vegetation. Full article