Search Results (1,042)

Wilson–Turner syndrome (WTS) is an X-linked developmental disorder associated with variants in the LAS1L gene, which plays a role in ribosome biogenesis. We report a 6-year-and-5-month-old boy presenting with growth retardation, early developmental delay, and mild scoliosis. Exome sequencing analysis identified a novel hemizygous LAS1L frameshift variant, c.2082dup (p.Leu697ProfsTer59), inherited from his asymptomatic mother that was absent from population databases. Functional analysis in HEK-293T cells suggested reduced protein expression with a partial loss of function effect, while structural modeling indicated potential alteration of the C-terminal region. The patient lacked classical WTS features, including craniofacial dysmorphism, truncal obesity, hypogonadism, and neuromuscular involvement. This case expands the phenotypic spectrum of LAS1L-related disorders and highlights the consideration of LAS1L variants in children with unexplained growth failure, scoliosis, or developmental delay, even in the absence of classical WTS features. Full article

On background cosmological scales, after subtraction of peculiar velocities and local bound-system motions, observed cosmological signals are redshifted rather than blueshifted. Yet, redshift alone does not distinguish the past lightcone of an expanding Universe from the future lightcone of a contracting one. In practice, the identification of the observed redshifted branch with the observational past is set primarily by electromagnetic radiation, whose retarded character is independently established in controlled physics, albeit over non-cosmological scales. From that perspective, the observed cosmological arrow is not separable from the causal/radiative prescription used to interpret the signals. This effective entanglement between the cosmological and the radiative arrows should nevertheless be distinguished from the notion of arrow used in the present work. Here instead, the relevant arrow is not thermodynamic but kinematic; it is defined by the symmetry or asymmetry of background lightcone observables under $\xi \leftrightarrow -\xi$, where $\xi \equiv ln(1+z)$ and z is the redshift—a criterion motivated directly by the time-reversal-symmetric special-relativistic longitudinal Doppler shift. Equivalently, the arrow considered here is the observed redshift/blueshift asymmetry of cosmological lightcone signals; retarded observations of an expanding FRW Universe are in the redshifted branch, whereas the opposite rapidity orientation would correspond to the blueshifted branch. This naturally suggests using rapidity-reversal symmetry as the redshift-space no-arrow condition when passing from special relativity (SR) to Friedmann–Robertson–Walker (FRW) cosmology, where the empty Milne Universe is a bridging borderline case. In fact, the viewpoint advocated here is that $\xi$-symmetry/asymmetry is practically more fundamental than t-symmetry/asymmetry simply because the former is more readily related to cosmological observables. It is shown here that generic non-empty FRW Universes possess an intrinsic $\xi$-asymmetry already at the background level, independently of entropy, coarse-graining, structure growth, or a Past Hypothesis. Full article

One of the keys to improving feed conversion rates in Precision Livestock Farming (PLF) is the early identification of growth impediments. However, the swine farming data collected by Electronic Feeding Station (EFS) are often disorganized and lack effective labeling. Data from healthy pigs are frequently intermixed with that from sick pigs, leading to label leakage and survivor bias in models, particularly when age is included as a feature. To address these known issues, this study breaks away from traditional modeling methods. First, we clean and classify the time-series data from electronic feeding stations, using age-cohort baselines as one of the criteria for determining high and low productivity, thereby avoiding problems such as label leakage. Next, we construct a high-dimensional feature matrix that captures dynamic derivatives such as feeding acceleration and weight gain acceleration, which together serve as behavioral feature fingerprints. To test the system, we optimized the mixed-model algorithm and evaluated the model based on behavioral deviations among individual pigs after removing all absolute age labels. Our results indicate that the full-feature model achieved an ROC-AUC of 0.778 and an F1-score of 0.4137 at the optimal threshold. Interestingly, SHAP attribution analysis revealed that “intake peer deviation,” “Cumulative Intake and Lifetime Avg Intake,” and “feeding acceleration” served as precursors to low productivity and growth retardation in this dataset, with these factors proving more significant than absolute feed intake or age. Our ablation experiments confirmed that a model based solely on behavioral features (excluding age labels) maintained an ROC-AUC of 0.773, successfully decoupling pig growth performance from growth stage. Our model can detect changes in feeding dynamic signatures at an average of 12.3 days, thereby providing insights for pig growth assessment, health monitoring, or more informed culling decisions. Full article

Rhizoctonia solani is a necrotrophic phytopathogenic fungus that causes disease in various crops. In agriculture, many crops suffer from root or seedling rot caused by this soil-borne pathogen, whereas cabbage and rice develop lesion-like symptoms on aboveground tissues. Diseases caused by R. solani are generally controlled using chemical fungicides; however, environmentally friendly alternatives are needed for sustainable agriculture. In this study, we evaluated the efficacy of lecithin, a mixture of phospholipids previously registered in Japan as an agrochemical for controlling cucumber powdery mildew, against Rhizoctonia diseases. In cabbage, foliar spraying of 0.2–1.0% soybean lecithin effectively suppressed leaf symptoms caused by R. solani isolate RhiCa-2, which was identified as AG-1 IB. In rice and Brachypodium distachyon, 0.2–1.0% lecithin significantly suppressed leaf symptoms induced by R. solani AG-1 IA. Hyphal staining of inoculated leaves revealed reduced hyphal density on lecithin-treated leaves. Consistently, hyphal growth of R. solani on cellophane placed on water agar was retarded by lecithin treatment. However, 5.0% lecithin induced phytotoxicity in B. distachyon. Egg yolk-derived lecithin also exhibited disease-suppressive activity in cabbage and B. distachyon, with efficacy comparable to that of soybean lecithin under the conditions tested. These results suggest that lecithin suppresses foliar infection by R. solani, at least in part, through direct inhibitory effects on fungal hyphae, and may serve as a potential alternative material for disease control in sustainable crop production. Full article

In recent years, synergistic effects between inorganic clay minerals (e.g., montmorillonite, sepiolite, kaolinite) and bio-based flame retardants (e.g., chitosan-based, lignin-based, phytate-based) have achieved certain progress in the area of polymer flame retardancy. The effects of bio-based flame retardants are exerted through mechanisms such as catalytic char generation and vapour-phase hindrance. However, they have limitations when used alone, including insufficient thermal stability and the need for a high dosage. Inorganic clays form physical barriers through their layered or tubular structures. The high thermal stability of these structures suppresses heat and mass transfer, thereby offsetting the shortcomings of bio-based flame retardants. This synergistic combination greatly improves the flame retardancy of polymer composites, often strengthening their mechanical performance in the process. It therefore offers great potential for the design of multifunctional, eco-friendly flame-retardant polymer composites. Nevertheless, a systematic review of the synergistic mechanisms, fabrication approaches and application progress of different inorganic clay minerals when combined with various bio-based flame retardants is still lacking. Therefore, this article offers a comprehensive review of the current developments of synergistic systems that incorporate various primary clays, such as sepiolite and montmorillonite, with bio-based flame retardants for usage in polymers. Before this, the synergistic flame-retardant mechanism and the key preparation techniques of the composite system were explained in detail. Finally, this article puts forward solutions to the current challenges and sets out prospects for innovation in the designing of flame-retardant materials and the optimisation of processes. The aim is to promote the sustainable growth of efficient, eco-friendly flame-retardant materials. Full article

The microsporidian parasite Enterocytozoon hepatopenaei (EHP) is a major pathogen causing severe growth retardation in shrimp, leading to substantial economic losses in global aquaculture. To address the urgent need for accurate, rapid, and field-deployable diagnostic tools for EHP, this study developed a novel one-pot detection platform by integrating asymmetric Enzymatic Recombinase Amplification (aERA) with a PAM-independent CRISPR/Cas12a system (AYERA-Cas12a) based on ssDNA activation. This design circumvents the compatibility challenge between isothermal amplification and CRISPR activity in a single tube by generating single-stranded DNA amplicons that activate Cas12a without requiring a PAM sequence. The assay operates at a constant temperature of 46 °C and completes detection within 15 min. It achieves a sensitivity of 10 copies/μL, equivalent to qPCR, and shows no cross-reactivity with six other prevalent shrimp pathogens. Validation using 56 clinical shrimp (Litopenaeus vannamei, L. vannamei) samples demonstrated complete agreement with qPCR results. With its simple procedure, isothermal conditions, and clear endpoint fluorescence readout under blue light, the AYERA-Cas12a platform is suitable for point-of-care testing (POCT). This work provides a user-friendly tool for the on-site surveillance and early diagnosis of EHP, offering significant potential for improving disease management in shrimp farming. Full article

Global warming-triggered heat stress severely restricts plant growth and crop productivity. Peanut (Arachis hypogaea L.), a vital oilseed and cash crop that is susceptible to high temperatures throughout its growth cycle, exhibits inhibited peg and pod development, growth retardation, and premature leaf senescence under heat stress, which ultimately causes substantial yield losses. Heat shock factors (Hsfs) serve as core regulatory modulators of plant abiotic stress tolerance, among which the HsfB subfamily exerts a critical function in thermotolerance modulation. Nevertheless, the biological functions of peanut HsfB genes remain largely uncharacterized. In the present study, a total of 16 HsfB subfamily members were identified from the peanut genome, possessing highly conserved gene structures and protein motifs. Phylogenetic analysis revealed that the peanut AhHsfB genes are classified into four distinct subfamilies. Chromosomal localization analysis indicated that these 16 AhHsfB genes are unevenly distributed across nine peanut chromosomes. Transcriptomic profiling demonstrated that the transcript levels of AhHsfB genes were significantly upregulated by 6- to 120-fold upon heat stress exposure. Subcellular localization and transcriptional activity assays further validated that AhHsfB1-5A is a nucleus-localized protein with intrinsic transcriptional activation activity. Ectopic overexpression of AhHsfB1-5A in Arabidopsis thaliana remarkably enhanced seed germination ability and antioxidant capacity under heat stress conditions, with a maximum 18.84% increase in green seedling rate. This study systematically characterizes the HsfB subfamily in peanut and elucidates the positive regulatory role of AhHsfB1-5A in plant thermotolerance. These findings deepen our understanding of the role of HsfB and provide valuable genetic resources for molecular breeding of heat-resistant peanut varieties. Full article

Magnesium hydroxide is attracting growing interest as a versatile, halogen-free flame retardant, and this review surveys its production routes, structure–property relationships and use in polymer systems from commodity polyolefins to advanced bio-based materials. Industrial Mg(OH)₂ is still predominantly obtained from mining or hydration of MgO, but increasing attention is being devoted to recovery from seawater and saltwork brines, where precipitation from Mg²⁺-rich streams followed by controlled rehydration or direct precipitation yields fine, high-purity powders suitable for flame retardant use and simultaneously valorizes saline wastes. In parallel, hydrothermal synthesis has been extensively explored to tailor particle size and morphology by adjusting the precursor, solvent, temperature and time, enabling high-surface-area Mg(OH)₂ or MgO with narrow size distributions that are attractive for high-performance composites also evaluated via ball milling, crushing and refining. More recently, process intensification strategies such as microwaves and ultrasounds have been proposed to shorten reaction times, lower temperatures and better control nucleation and growth, opening paths toward energy efficient production of structured Mg(OH)₂ from both conventional and brine-derived precursors. The second part of the review analyzes how the intrinsic endothermic decomposition and basic character of Mg(OH)₂ can be utilized across a broad range of polymer matrices and how surface functionalization strategies extend its applicability. In addition to “as received” powders, stearic acid and other fatty acids, metal soaps and various organic coupling agents are widely used to render the surface more hydrophobic, enhance dispersion and interfacial adhesion, and in some cases introduce additional char-forming or barrier functionality. In terms of the application, the review methodically synthesizes and contrasts fire and mechanical data for Mg(OH)₂-containing polyolefins (HDPE, LLDPE, PP and EVA) utilized in cables and building products, expandable polymers and foams, biopolymers (PLA and PBS), and elastomers. The review places particular emphasis on the balance between loading level, processability, flame performance and mechanical integrity. This review aims to provide a comprehensive framework for designing next-generation Mg(OH)₂-based flame-retardant systems for both conventional and emerging polymer technologies. To this end, it integrates advances in sustainable feedstocks, controlled synthesis and surface engineering with the rapidly expanding application space. Full article

Ether lipids in varying amounts are membrane constituents and storage material in the protist and animal kingdoms, but are largely absent from fungi and plants. Their biosynthesis pathway starts in the peroxisome and involves a set of well-conserved enzymes. Only one step, the reduction of alkyl-dihydroxyacetone-phosphate to alkyl-glycerol-3-phosphate, is mediated by so-called short-chain dehydrogenases/reductases, which are members of huge protein families. Here, using GFP fusions, we identify a peroxisomal enzyme in Dictyostelium, as well as a highly related protein residing in the endoplasmic reticulum. Single-gene knockouts indicate that these enzymes largely compensate for one another, suggesting a flexible redistribution of lipid metabolites between these organelles. The double knockout, however, is severely affected in ether lipid composition and displays a clear growth retardation. The defects can also be reverted by expression of the cognate yeast enzyme, demonstrating conservation of this metabolic step across kingdoms of life. Full article

During the early growth stage, lambs are highly susceptible to pathogenic microbial invasion due to an underdeveloped intestinal structure, unstable microbial colonization, and immature mucosal immune function, leading to diarrhea, growth retardation, and elevated mortality factors that severely constrain the production efficiency and economic viability of the sheep industry. This study aimed to compare the regulatory effects of compound yeast culture (CYC) and yeast polysaccharides (YPs) on intestinal barrier function in Mongolian male lambs and clarify their underlying molecular mechanisms. Eighteen lambs were randomly assigned to three groups (n = 6/group): control group (basal diet), CYC group (40 g/kg), and YP group (3 g/kg). After a 30-day feeding trial, intestinal histomorphology, tight junction proteins, immune signaling pathways, and gut microbiota were analyzed. The results showed that both additives improved intestinal villus morphology, and CYC markedly increased the villus height/crypt depth ratio (p < 0.05). At the mechanical barrier level, CYC upregulated the protein expression of occludin, claudin-1, and ZO-1, whereas YPs increased occludin and ZO-1 expression (p < 0.05). Immunologically, CYC inhibited intestinal inflammation via the TLR4/TRAF6/MyD88/NF-κB pathway, increasing interleukin-10 (IL-10) and secretory immunoglobulin A (sIgA) while decreasing pro-inflammatory cytokines. YPs exerted similar anti-inflammatory effects through the TLR2/MyD88 pathway. Microbial analysis indicated that both additives increased the relative abundance of beneficial bacteria including Eubacterium, Bacillus, and Succinivibrio, while reducing the potential pathogen Mogibacterium. Spearman correlation analysis revealed that Mogibacterium was positively correlated with TNF-α and negatively correlated with occludin expression. In conclusion, CYC and YPs effectively enhance intestinal mechanical, immune, and biological barriers via different TLR-mediated pathways and microbial modulation. Both natural additives have great application potential for improving lamb health, reducing antibiotic dependence, and promoting sustainable green animal husbandry. Full article

Inflammatory bowel disease (IBD) with an onset in childhood is characterized by a more extensive phenotype, a more aggressive clinical course, and a higher risk of long-term complications, including growth retardation, compared to adult-onset disease. While tumor necrosis factor-alpha (TNF-α) inhibitors are the cornerstone of therapy, achieving sustained remission in children is often hindered by unique pharmacokinetic challenges, such as accelerated drug clearance and a higher propensity for immunogenicity. This review explores the evolving role of therapeutic drug monitoring (TDM), specifically the paradigm shift from reactive to proactive strategies. While proactive TDM remains a subject of debate in adult IBD, emerging pediatric data strongly support its routine use to optimize treatment durability and prevent secondary loss of response. Evidence-based target trough concentrations for pediatric patients are critical for achieving mucosal healing: 8–13 µg/mL at week 6 and >5–7 µg/mL during maintenance for infliximab, and >13–14 µg/mL post-induction for adalimumab. Beyond clinical outcomes, this review emphasizes the economic viability of proactive TDM, which has been shown to reduce total healthcare expenditures by 18–30% by minimizing hospitalizations and avoiding premature treatment switches. By integrating pharmacological data with clinical pathways, proactive TDM serves as an essential tool for personalized medicine, ensuring safer and more cost-effective management of pediatric IBD. Full article

The fatigue performance of titanium alloys is a critical determinant of the service life and structural integrity for aerospace and marine engineering components. But within the framework of damage tolerance design, resistance to fatigue crack propagation is regarded as a key indicator governing the fatigue performance of these engineering structures. In previous work, while the general fatigue performance of Ti–6Al–4V-0.55Fe alloy has received systematic study, targeted research focusing on its resistance to fatigue crack propagation remains limited. Therefore, in this work, compared with Ti–6Al–4V ELI alloy, the fatigue crack propagation behavior and fracture mechanism of Ti–6Al–4V-0.55Fe alloy with a duplex microstructure were systematically investigated. The results show that when ∆K < 12.75 MPa⋅m^1/2, Ti-6Al-4V-0.55Fe alloy demonstrates superior resistance to fatigue crack propagation. Fractographic analysis indicates that the primary difference between the two alloys lies in the stage of crack initiation and early propagation. This behavior is attributed to the addition of trace Fe, which enhances α/β boundary resistance and thereby retards crack growth. Moreover, crack propagation of TC4-F alloy is also slowed by the increased path length from bypassing the α_p phase. Full article

Histamine is a significant risk factor in brown fishmeal and negatively affects histamine-sensitive fish species. The present study aimed to explore the potential protective effects of dietary sanguinarine supplementation against the adverse impacts of excessive dietary histamine on growth and intestinal and hepatic health in American eels. Four experimental diets were formulated: the basal diet (control diet), the basal diet supplemented with 500 mg kg⁻¹ of histamine (HH diet), and the HH diet supplemented with 100 mg kg⁻¹ and 200 mg kg⁻¹ of sanguinarine (HH+SAN100 and HH+SAN200 diets, respectively). After a 10-week feeding trial, both sanguinarine supplementation diets alleviated HH-diet-induced growth retardation, reduced feed utilization, serum dyslipidemia, and hepatic and intestinal structural damage; decreased hepatic and intestinal antioxidant capacity; increased intestinal and hepatic malondialdehyde levels; inhibited intestinal lipase activities; elevated intestinal pro-inflammatory-related gene abundance; and lowered intestinal anti-inflammatory-related gene expression. Furthermore, the HH+SAN100 diet partially ameliorated HH-diet-induced gut microbiota dysbiosis and intestinal metabolic disturbances. Collectively, these findings identify sanguinarine as a functional dietary additive that mitigates histamine-associated growth suppression and intestinal and hepatic injury in American eels, promoting the sustainable utilization of brown fishmeal in American eel culture. Full article

Enhancing the freeze–thaw resistance of cement-based materials in a green and efficient manner is crucial for hydraulic structures in cold regions. This study investigated the effects of soybean antifreeze protein (AFP) on the freeze–thaw durability of cement mortar through mechanical testing, low-temperature microscopy, NMR analysis, and frost-heaving stress monitoring. The results show that AFP improves freeze–thaw durability, with 0.5% dosage outperforming 1.0%. Relative to the control, the relative ice content at −20 °C decreased from 62.81% to 40.01%, and frost-heaving stress declined from 321.15 kPa to 123.04 kPa. Microscopy and pore structure analyses revealed that AFP transforms ice crystals from needle-like to fine granular forms, inhibiting ordered growth and retarding pore coarsening. A frost-heaving stress model based on the Gibbs–Thomson effect and ice-crystal fractal characteristics indicated that AFP suppresses stress development by reducing effective ice formation, weakening stress transfer, and increasing ice-crystal boundary complexity. This study offers insights for developing green antifreeze admixtures for cement-based materials in cold regions. Full article

Avibacterium paragallinarum (A. paragallinarum) is the primary causative agent of infectious coryza in chickens. Infection often leads to growth retardation in broilers and a 10% reduction in egg production, reaching over 40% in laying hens. The problem is particularly severe under intensive farming conditions, significantly jeopardizing global poultry health and farming profitability. From a ‘One Health’ perspective, this not only disrupts the stability of the food supply chain, but also increases antibiotic usage due to disease prevention and control needs, thereby aggravating antimicrobial resistance (AMR) and posing a global public health challenge. This review systematically summarizes advances in the pathogenesis of A. paragallinarum and the protective immunity induced by subunit vaccines. It focuses on the infection mechanisms of A. paragallinarum, emphasizing its colonization strategies in the infraorbital sinus and nasal epithelium of chickens, and analyzes the roles of key virulence factors such as hemagglutinin and capsule in adhesion, colonization, and immune evasion. We integrate the tissue-specific pathogenesis of A. paragallinarum with the role of respiratory commensal microbiota in facilitating infection, providing an in-depth analysis of the bacterium’s key immune evasion strategies, thus offering novel insights into host–pathogen-microbiome interactions. Concurrently, to the best of our knowledge, this review provides the first comprehensive overview of current developments in subunit vaccines and their immunoprotective properties, with special attention to limitations in eliciting mucosal immune responses. By delving into the pathogen-host interaction mechanisms, this review aims to inform the optimization of subunit vaccine design and immunization strategies. Ultimately, it seeks to establish a theoretical basis and practical framework for precise control of A. paragallinarum. Full article