Search Results (1,045)

This study explores the use of endophytic fungi for the biocontrol of harmful aflatoxins (AFTs) in maize (Zea mays L.). The main objective of this study was to evaluate the effects of fungal pathogens and biocontrol agents on the corn seed germination and growth of seedlings under controlled conditions. Experiments were conducted under laboratory conditions in a growth chamber and in a greenhouse to assess the influence of environmental factors on seed performance and treatment efficacy. The growth chamber provided uniform conditions for physiological assessment while the greenhouses represented more realistic field conditions. Corn kernels were sown in sterile pots inside the growth chamber at standard conditions or in the greenhouse at controlled conditions and four treatment groups were established: untreated control seeds, seeds treated with non-AFT-producing (non-aflatoxigenic) strains (Trichoderma harzianum, T. asperellum and Aspergillus niger), seeds inoculated with AFT-producing (aflatoxigenic) strains (A. flavus and A. parasiticus), and seeds co-inoculated with both aflatoxigenic and non-aflatoxigenic strains (A. flavus and A. parasiticus with T. harzianum, T. asperellum or A. niger). High-performance liquid chromatography was utilized to detect and analyze the presence of AFTs. Co-culturing of A. flavus with T. harzianum resulted in a significant decrease in AFT levels, achieving a relative reduction of 99.3% compared to aflatoxigenic treatments alone. Among the isolates tested, T. harzianum and T. asperellum were the most effective at lowering AFT production of the aflatoxigenic strains, reducing the 5120 ± 560 µg/kg AFT level produced by A. flavus alone to 50.1 ± 1.10 and 63.1 ± 3.1 µg/kg, respectively. A. flavus negatively affected germination and early growth, whereas T. harzianum significantly enhanced both parameters. This study demonstrates that non-aflatoxigenic Trichoderma isolates can effectively mitigate AFT contamination and improve seedling growth, highlighting their potential as effective. sustainable, and locally adopted biocontrol agents for Pakistan’s chronic AFT problem under diverse environmental conditions—an area with minimal prior research and high national relevance. Full article

A comprehensive assessment of the effect of different light spectra on the growth, development, and nutritional composition of Mentha longifolia L. cv. “Vesenniy Aromat” (mint) and Melissa officinalis L. cv. “Limonnyy Aromat” (lemon balm) grown in hydroponic conditions in closed artificial agroecosystems was conducted. The growing period was 75 days for mint and 87 days for lemon balm. The photon flux density (PFD) in the range of 400–800 nm was ~140 µmol·m⁻²·s⁻¹, and the light period was 16 h. Five lighting options and four spectral color ratios were used in the treatments—blue (B), green (G), red (R), and far red (FR), and 3:66:27:4 (HPL (control)); 16:42:39:3 (White LED); 96:3:1:0 (Blue LED); 1:1:98:0 (Red LED) and 25:3:72:0 (Red + Blue LEDs)—in a growth chamber for cultivation with controlled environmental conditions. Under White LED, M. longifolia L. plants were compact, with a large number of leaves and high plant biomass. The effect of Red + Blue LEDs had a general trend for M. longifolia L. and M. officinalis L. in terms of improving plant morphology (leaf area, number of leaves, and plant biomass), elemental composition (contents of potassium, magnesium, calcium, and phosphorus) and reducing the accumulation of nitrates in the plants. Blue spectrum lighting significantly affected the content of leaf pigments, quercetin, rosmarinic acid, and essential oils of mint and lemon balm. Red spectrum lighting significantly reduced the accumulation of nitrates in the vegetative mass of plants. Precise regulation of metabolic processes, taking into account the spectral quality of light, can contribute to improving the economic efficiency of the growth, development, and productive potential of mint and lemon balm grown under controlled conditions. Full article

The potato chip processing (PCP) industry generates huge amounts of wastewater heavily polluted with organic matter and nutrients. The current treatment technology of PCP wastewater uses dissolved air flotation (DAF) and an activated sludge sequential batch reactor (SBR); both consume large amounts of chemicals and represent energy-intensive systems. This study explores the utilization of algae for the sustainable treatment of PCP wastewater, nutrient recovery, and algal biomass production. Conical flasks (1-L) and 6-L transparent plastic bottles were used as lab-scale algae photobioreactors (APBRs). Raw wastewater, an anaerobically pre-treated effluent and a DAF–SBR or shortly SBR effluent were used in the first, second, and third APBR. Three feed volumes from each source (150 mL, 300 mL, and 500 mL for first and second APBR and 400 mL, 600 mL, and 800 mL for third APBR) to a fixed volume of algal seed (200 mL) were tested to select the optimal feed volume and harvest time using a 1-L APBR. System performance and impact of water characteristics on quantity and quality of algal biomass were explored at pre-selected feed volume and harvest time in 6-L APBRs. All experiments were carried out in a growth chamber with continuous light (148.75 μmol.m⁻².S⁻¹). The results showed that 150 mL is the optimal feed volume for the first and second APBR at 10 days and 9 days growth cycles. An amount of 500 mL and 6 days were selected as the optimal feed volume and growth cycle for the third APBR. The average dry biomass yields at the pre-selected optimal conditions were 65.3 ± 11.4, 69.9 ± 12.0, and 100.6 ± 11.7 mg/L.d in the first, second, and third APBR, respectively. The first APBR achieved removals of 99.2 ± 0.4%, 98.7 ± 0.8%, 89.1 ± 4.3%, and 97.5 ± 1.4% for turbidity, COD, TKN, and TP, respectively, on average. Corresponding removal in the second APBR is 97.6 ± 2.6%, 91.6 ± 7.5%, 93.6 ± 4.5%, and 96.1 ± 1.4%, respectively, while the third APBR achieved 98.5%, 76.2%, and 97.0%, respectively. Additionally, the results of protein content and amino acids profiles indicate significant impacts of feed water quality on both parameters. The protein content was 30.64%, 32.53%, and 35.65% in the first, second, and third APBR, respectively. Similarly, the amino acids profile indicated a significant higher percentage of the amino acids in the third reactor compared with the first and second reactor. Full article

Brush seals, as a fundamental dynamic sealing technology in the aerospace and energy propulsion industries, require performance enhancement through instantaneous adjustment of the friction coefficient and force analysis of brush filaments. This paper establishes an instantaneous friction coefficient correction method based on the open volume between bristles and the backing plate. The downstream section of the double-row brush wire (2.6 mm) was quantitatively identified as the maximum leakage point, and it was found that the vortex characteristic length in the downstream area is approximately 1–3 times the bristle gap, with an increasing pressure ratio enhancing downstream turbulence and reducing gas leakage. A cantilever beam structural model was developed to assess the motion, force, and hysteresis properties of a single filament. Additionally, a porous medium model was utilized to elucidate the flow field and temperature distribution within the seal. The results suggest that the lag angle increases linearly over the first one-third of the brush wire’s length from the free end to the fixed end and is directly proportional to the pressure difference ΔP, reaching a maximum of 10.18°. The viscous drag causes the radial force y-component F_xy to increase and then decrease near the free end. The rear baffle contact force, F_b, shows variable peaks at two-thirds of the filament length. The displacement at the brush filament’s free end, the deflection angle, and the bending moment are directly proportional to the pressure differential. As pressure increases, the deformed region propagates toward the fixed end, and the maximum displacement at the free end of the brush wire reaches 13.04 mm. The leakage rate increases nearly linearly with ΔP and its deformation, reaching a maximum of 0.00849 m²/s. The pressure gradient growth rates of 164%, 73%, and 29% at the front baffle corner demonstrate that adding pressure chambers on front and rear baffles is optimal for high-pressure scenarios (ΔP > 0.3 MPa), while the formation of vortices between bristles and rotor reduces tip friction force and front-row turbulent disturbance, providing design guidance for extending seal service life. Full article

Chitosan-based silver nanoparticles (Ch-AgNPs) are emerging as promising antimicrobial materials with potential applications in crop protection. This study evaluated the formulation-dependent antimicrobial activity and plant compatibility of Ch-AgNPs synthesized from chitosan extracted via different routes from shrimp shells. Antibacterial activity was assessed against representative Gram-negative and Gram-positive model bacteria (Escherichia coli and Staphylococcus aureus), as well as phytopathogenic bacteria (Xanthomonas campestris, Pseudomonas syringae), using disk diffusion assays. Antifungal activity was evaluated against Fusarium graminearum in vitro and in a controlled growth chamber. All formulations exhibited concentration-dependent antibacterial activity, with L10 and L20 formulations derived from optimized lactic acid-based extraction routes and DP4 derived from an inorganic deproteinization-based extraction route showing the highest efficacy at 1.0 mg/mL. Strong antifungal activity was observed, particularly for L10 and DP4, achieving mycelial growth inhibition of 92% and 84%, respectively, at 1.0 mg/mL. Seed germination and seedling growth assays confirmed that all formulations were non-phytotoxic at 1.0 mg/mL, with L10 and DP4 significantly enhancing germination parameters and early plant growth. Under controlled conditions, these formulations also reduced the incidence and severity of crown and root rot in spring wheat caused by F. graminearum. These findings demonstrate that optimized Ch-AgNP formulations combine antimicrobial activity with plant compatibility, highlighting their potential for crop protection, pending further environmental safety and agronomic validation under field conditions. Full article

Optimizing plant density and vegetative growth duration is important for improving productivity in controlled-environment medicinal cannabis cultivation. Although both factors strongly influence canopy development and yield, their combined effects under modern high-intensity LED lighting, and particularly their consequences for cannabinoid uniformity across the canopy, remain insufficiently characterized. This study examined how planting density and vegetative duration influence plant growth, yield, and cannabinoid concentration in Cannabis sativa L. (strain ‘Fat Banana’) grown under controlled environment conditions, high-intensity LED lighting and precision fertigation. Two vegetative durations (10 and 28 days) were evaluated in separate but identical controlled-environment chambers under broad-spectrum high-intensity LED lighting and automated precision fertigation on rockwool substrate. The 10-day regime compared 8, 14 and 18 plants m⁻²; the 28-day regime compared 6, 8 and 10 plants m⁻². Each combination was replicated across two independent cultivation cycles, and because density levels differed between regimes, direct between-regime comparisons were restricted to the shared density of 8 plants m⁻². Extending the vegetative phase from 10 to 28 days increased plant height, stem diameter and internodal length. Area-based yield increased strongly with density, reaching 1091 g m⁻² at 18 plants m⁻² under the 10-day regime and 1009 g m⁻² at 10 plants m⁻² under the 28-day regime. Apical biomass exceeded basal biomass, but total THC concentration did not differ significantly among planting densities, vegetative durations or canopy positions. Higher planting densities combined with shorter vegetative periods can therefore increase area-based productivity while maintaining stable THC concentration under high-intensity LED cultivation. Full article

The study assessed the impact of climate change, aphid infestation and drought stress on winter wheat (Triticum aestivum L.) and the performance of English grain aphid (Sitobion avenae) under abiotic stress in controlled environmental conditions. To understand wheat and aphid interactions under different climatic condition, wheat plants were grown in controlled climatic chambers simulating present (400 ppm CO₂, 19.8 °C, RH 69.2%) and future (700 ppm CO₂, 23.4 °C, RH 67.5%) scenarios, combined with biotic stress (aphid) and abiotic stress (drought). Climate change effects combined with other stress factors are expected to alter crop physiology and insect biology. The results showed that aphid performance was significantly enhanced under future climatic conditions, with higher fecundity (56%), and a shortened or faster developmental time. As for wheat structural growth, above-ground biomass improved by up to 80% under future climate. However, its physiological efficiency, water content and photosynthetic efficiency were significantly reduced under the combined biotic and abiotic stresses. The study demonstrates that climate change may increase wheat plant growth under controlled conditions, yet it simultaneously boosts the shift in pest attacks and intensifies stress impacts, which eventually threaten wheat productivity. The findings emphasize the improvement of wheat varieties and pest-resistant strains capable of withstanding future climatic conditions. Full article

In magnetron sputtering-based gas aggregation sources, nanoparticle formation and yield are strongly influenced by the flow-regulated transport and residence time of particles within the condensation chamber. However, the effect of internal geometric parameters on flow structure and nanoparticle growth is not well understood. In this study, computational fluid dynamics (CFD) coupled with a discrete phase model (DPM) is employed to investigate how magnetron radius affects flow characteristics, particle transport, and their implications for nanoparticle formation. The results show that increasing the magnetron radius significantly enhances axial flow uniformity and suppresses vortex structures near the inlet. This shift from radial diffusion-dominated to primarily axial transport effectively reduces particle trapping and wall deposition. Furthermore, the regulation of flow structure modifies particle residence time distributions, which is considered a key factor associated with nanoparticle growth potential and size evolution in gas-phase synthesis. Larger magnetron radii promote more stable transport pathways and improve particle transmission efficiency, thereby improving particle transmission efficiency and providing more favorable conditions for nanoparticle formation. These findings indicate that geometric optimization can simultaneously enhance transport efficiency and influence the conditions potentially favorable for particle growth, providing valuable guidance for the design of high-yield nanoparticle synthesis systems. Overall, this work provides insight into how flow field characteristics influence nanoparticle transport and potential growth behavior, offering a foundation for optimizing magnetron sputtering-based nanoparticle synthesis. Full article

Growth chambers are vital in controlled environment agriculture (CEA), enabling precise regulation of environmental conditions for year-round crop production, especially in urban areas with limited arable land. This study retrofitted a nonfunctional cold room into a plant growth chamber with controlled temperature, humidity, and CO₂ levels to evaluate lettuce (Lactuca sativa) growth under three LED treatments: broad-spectrum white, combined white and far-red, and narrow amber (598 nm). Environmental parameters were controlled at 21 °C during the day and 19 °C at night, with 65% relative humidity, and 800 ppm CO₂. After 40 days, plants under combined white and far-red LEDs produced the tallest shoots (21.8 ± 0.3 cm) and highest leaf count (23.7 ± 0.9). No significant differences were observed among treatments for fresh mass, dry mass, head diameter, or relative chlorophyll content. The findings demonstrated the feasibility of retrofitting a nonfunctional cold room into a controlled environment growth chamber capable of supporting lettuce cultivation under the tested conditions. Full article

To investigate the effects of water-saving irrigation and different straw retention methods on soil CH₄ and N₂O emissions from paddy fields in cold regions and their potential underlying mechanisms, a field experiment was conducted in Qing’an City, Heilongjiang Province. Two water management regimes were set, combined with four straw retention treatments. The static chamber-gas chromatography method was used to monitor CH₄ and N₂O emission fluxes during the entire rice growth period. Meanwhile, soil pH, oxidation–reduction potential (Eh), dissolved oxygen (DO), and dynamic changes in carbon and nitrogen substrates were measured, and the global warming potential (GWP) and greenhouse gas emission intensity (GHGI) were comprehensively evaluated. The results showed that controlled irrigation significantly increased soil dissolved oxygen content and oxidation–reduction potential. Compared with conventional flooding irrigation, total CH₄ emission decreased by more than 50%, while N₂O emission increased by 1.5–2.5 times, exhibiting an obvious divergent correlation with the two gas emission fluxes. Among different straw retention methods, organic fertilizer returning and direct straw returning significantly promoted CH₄ emission by supplying easily decomposable organic carbon. In contrast, biochar, due to its stable carbon structure and favorable pore properties, inhibited CH₄ emission without significantly stimulating N₂O emission. The treatment of controlled irrigation combined with biochar returning (CB) achieved the lowest global warming potential and greenhouse gas emission intensity at 7230.82 kg CO₂-eq/hm² and 0.8054 kg CO₂-eq/kg, respectively, while maintaining high rice yield. Path analysis based on soil physicochemical properties and emission fluxes further revealed that Eh and DO were significantly negatively correlated with CH₄ emission but positively correlated with N₂O emission. Path inference from flux and substrate data indicated that carbon and nitrogen availability were the key factors limiting the denitrification process. In conclusion, the combined application of controlled irrigation and biochar returning can realize the synergistic effect of stable yield and emission reduction in cold-region paddy fields by improving soil aeration and regulating the transformation of carbon and nitrogen substrates, providing a scientific basis for establishing a green and low-carbon rice production technology system for black soil in cold regions. Full article

The increasing use of recycled polypropylene (rPP) in technical and outdoor applications requires strategies to limit photo-oxidative degradation while maintaining adequate performance after reprocessing. In this work, the photo-oxidative stability of rPP films was investigated under accelerated weathering conditions, focusing on the effect of a commercially available additive, Nexamite^® R201 (NEX), previously shown to partially restore PP molecular weight after reprocessing. Films of rPP and rPP containing 5 wt.% NEX were produced by cast extrusion and exposed to cyclic UVA irradiation and water condensation in a QUV chamber, and the evolution of the functional and structural degradation of the materials was monitored as a function of aging time. Spectroscopical analyses showed progressive oxidation in both systems, with carbonyl growth starting after an induction period of about 200 h. A faster increase in the carbonyl index was observed for rPP containing NEX, indicating that the additive does not improve chemical oxidative resistance under the adopted conditions. However, NEX significantly enhanced the retention of mechanical properties during aging, with higher elongation and stress at break compared with unmodified rPP, thus delaying embrittlement. Overall, the results show that the investigated additive effectively mitigates the loss of mechanical integrity during photo-aging, likely as a consequence of the macromolecular restructuring induced during reprocessing. Full article

Background: Glufosinate ammonium (GLA) is a widely used herbicide, yet potential neurodevelopmental risks related to paternal exposure before conception remain insufficiently defined. Methods: In this study, adult male C57BL/6J mice received GLA at 0.2 mg/kg·day for 10 consecutive weeks and were then mated with unexposed females to generate F1 offspring. Offspring growth was monitored, and neurobehavior was assessed at 5 weeks of age. Results: In behavioral tests, female offspring showed reduced social novelty preference in the three-chamber test and impaired spatial learning and memory in the Morris water maze test, while open field, elevated plus maze, and rotarod performance were not altered. Male offspring showed no clear group differences in these memory-related endpoints. Golgi staining revealed reduced dendritic complexity and spine density in the hippocampus and prefrontal cortex. Glial markers were elevated, and neuronal marker changes showed region-dependent shifts. TUNEL staining indicated increased apoptosis during embryonic development and persistent apoptotic signals in the juvenile prefrontal cortex, accompanied by cytokine imbalance with increased IL-1β and decreased IL-10 in the hippocampus. Conclusion: These results suggest that paternal preconception GLA exposure is associated with selective memory-related behavioral deficits in juvenile offspring and with convergent glial, inflammatory, and apoptosis-related brain changes. These findings support the consideration of paternal exposure in developmental risk assessment frameworks. Full article

This study evaluated the effects of light spectral quality on shoot yield and essential oil of Tagetes erecta L. cultivated in controlled growth chambers under three LED lighting treatments with different red, blue, and white wavelength ratios and a constant 16 h photoperiod for up to 101 days. The F2 treatment (5 red:1 blue) produced yields of fresh shoots, early blooming flowers, and oils of 1586 ± 164 g/m², 569.77 ± 76.81 g/m², and 307 ± 31.7 mg/m², respectively. These values were significantly higher (p < 0.05) than those of the F1 treatment (white:red-phosphor), and represented increases of 1.37-, 1.26-, and 1.38-fold, respectively. Gas chromatography identified 30–31 compounds in the oil with three major constituents—(E)-β-ocimene (22.9–28.8%, highest under F3), (E)-myroxide (13.9–20.6%, highest under F1), and piperitone (7.3–9.6%, highest under F3). Essential oils inhibited from four to five of the seven tested microbial strains, with the notable activity against Escherichia coli and Candida albicans recorded in F2 and F1, respectively. These findings confirm that light spectral quality is a critical factor regulating flower, essential oil, and antimicrobial efficacy in T. erecta, demonstrating that optimized LED spectra offer a practical strategy to improve plant yield and phytochemical quality. Full article

Fine particulate matter PM_2.5 continues to pose a critical public health risk in Northern Thailand, particularly in Chiang Mai, where traditional filtration methods often face limitations in cost and efficiency for large-scale applications. This study introduces a novel “Evaporator Condensation Growth Particle Scrubber (ECGP)” designed to enhance the collection efficiency of sub-micron particles by enlarging their physical size through a pressure-driven growth mechanism. The ECGP system utilizes synergistic effects between solid nuclei, high relative humidity, and mechanical pressure modulation. The ECGP system integrates solid nuclei, ~95% relative humidity and mechanical pressure modulation within a single chamber. Using incense smoke as a PM surrogate, the process utilizes controlled adiabatic cycles to induce stable heterogeneous condensation. The results indicate that the integrated process effectively shifts particle size distribution, reducing the PM_2.5/PM₁₀ mass ratio from 1.00 to 0.83. This indicates that approximately 17.5% (with a standard deviation < 1% across 10 trials, p < 0.05) of the fine mass successfully transitioned into the larger, more filterable PM₁₀ fraction and exhibited high physical stability and resistance to re-evaporation, effectively overcoming the low-efficiency threshold (typically <10%) of standard mechanical scrubbers and cyclones for sub-micron dust. This study concludes that ECGP technology offers a promising, cost-effective alternative for improving indoor air quality in large public infrastructures by leveraging particle inertia for enhanced removal, providing a scalable solution to the persistent smog crisis. Full article

Background: Pacemakers enable continuous long-term surveillance of atrial fibrillation detected by implanted devices. Circulating biomarkers reflecting endothelial dysfunction, inflammation, and myocardial stress may help identify patients at risk for atrial fibrillation (AF) progression and higher arrhythmic burden. Methods: This analysis included patients from the prospective ACaSA study (NCT05127720) with a dual chamber pacemaker (Microport^® BOREA DR or TEO DR) and monitored weekly via remote monitoring technology (SMARTVIEW^®). Individuals with permanent AF or single-chamber systems were excluded. Baseline plasma concentrations of angiopoietin-2 (ANGPT2), growth differentiation factor-15 (GDF-15), fibroblast growth factor-23 (FGF-23), bone morphogenetic protein-10 (BMP10), and tumor necrosis factor–related apoptosis-inducing ligand receptor-2 (TRAIL-R2) were quantified using enzyme-linked immunosorbent assays. N-terminal pro-B-type natriuretic peptide (NT-proBNP) was measured using electrochemiluminescence immunoassay. Biomarkers were log₂-transformed, with values below assay detection limits imputed at half the lower limit of detection. Two endpoints were assessed following a 30-day blanking period: (1) progression to persistent AF, defined as ≥7 consecutive days with >99% daily AF burden, analyzed using Cox regression; and (2) AF burden, calculated as total AF time normalized to monitored days and categorized as <25%, 25–75%, or >75%, analyzed using multinomial logistic regression. Multivariable models were adjusted for age, sex, heart failure, diabetes, and prior myocardial infarction; Cox models were limited to age, sex, and heart failure due to fewer events. Results: A total of 223 patients were included (median age 75 years; 37.2% women). During follow-up, 28 patients (13.3%) progressed to persistent AF. Higher baseline ANGPT2 was the strongest predictor of progression (HR per doubling 1.83, 95% CI 1.27–2.66, p = 0.001), followed by GDF-15 (HR 1.52, 95% CI 1.03–2.24, p = 0.036). In the burden analysis, ANGPT2 demonstrated a pronounced graded relationship with arrhythmic load, with markedly increased odds of high (>75%) AF burden (OR 8.31, 95% CI 2.63–26.26, p < 0.001). GDF-15 independently predicted both medium (OR 2.05, p = 0.025) and high burden (OR 2.32, p = 0.037). NT-proBNP displayed a borderline association with high burden (OR 2.02, p = 0.061). No significant associations were observed for FGF-23, BMP10, or TRAIL-R2. Conclusions: In continuously monitored pacemaker patients, ANGPT2 and GDF-15 emerged as key biomarkers associated with AF disease severity. ANGPT2 was strongly linked to both progression to persistent AF and high AF burden, whereas GDF-15 consistently predicted higher AF burden and also contributed to risk of progression. These findings highlight endothelial and inflammatory pathways as potential markers of atrial disease progression. Full article