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Keywords = greenhouse studies

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24 pages, 777 KB  
Article
Effect of Bioeconomy Integration on the Transition from Traditional Livestock Farming to Circular Farming Models in Greece
by Stavros Kalogiannidis, Konstantinos Spinthiropoulos, Fotios Chatzitheodoridis, Dimitrios Parris and Angel Valsamopoulos
Conservation 2026, 6(2), 74; https://doi.org/10.3390/conservation6020074 (registering DOI) - 15 Jun 2026
Abstract
This study investigates the integration of bioeconomy principles in the Greek livestock sector, framing the transition from conventional farming toward a circular bioeconomy as a strategy for resource conservation and reduced environmental pressure. It assesses farmers’ awareness of bioeconomy principles, the adoption of [...] Read more.
This study investigates the integration of bioeconomy principles in the Greek livestock sector, framing the transition from conventional farming toward a circular bioeconomy as a strategy for resource conservation and reduced environmental pressure. It assesses farmers’ awareness of bioeconomy principles, the adoption of circular practices, and the associated economic and conservation-related performance. Data were collected through a structured questionnaire administered to 383 livestock farmers across the main livestock-producing regions of Greece and analyzed using descriptive statistics and multiple regression. Although respondents show substantial awareness, adoption remains incomplete, mainly because of high initial capital costs and insufficient financial incentives. Farmers implementing circular strategies reported gains in resource-use efficiency, waste minimization, and the conservation of soil, water, and biodiversity, particularly reduced greenhouse-gas emissions, while public subsidies and fiscal incentives emerged as the principal drivers of adoption. In applied terms, support should be prioritized for capital-intensive investments such as anaerobic digestion, manure and nutrient recovery, and water reuse, and the awareness–adoption gap is best closed through targeted subsidies and training. The findings offer concrete guidance for conservation-oriented agri-environmental policy supporting the green transition of livestock farming in Greece. Full article
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19 pages, 2621 KB  
Article
Assessment of Sustainable Mobility Planning in Lithuanian Cities: A Comparative Content Analysis of Sustainable Urban Mobility Plans
by Renata Činčikaitė
Urban Sci. 2026, 10(6), 328; https://doi.org/10.3390/urbansci10060328 (registering DOI) - 15 Jun 2026
Abstract
Road transport is one of the most significant sources of environmental pollution and greenhouse gas emissions; therefore, the development of sustainable mobility is becoming an important direction of urban transport policy. The objectives of the European Union’s transport policy encourage cities to plan [...] Read more.
Road transport is one of the most significant sources of environmental pollution and greenhouse gas emissions; therefore, the development of sustainable mobility is becoming an important direction of urban transport policy. The objectives of the European Union’s transport policy encourage cities to plan and implement measures that reduce the environmental impact of transport, improve transport conditions, and increase the availability of mobility alternatives. The aim of this study is to evaluate the planning of sustainable mobility development in Lithuanian cities by analysing sustainable urban mobility plans, the measures proposed in them, and their links to the needs of urban transport systems. The study applied descriptive statistics, comparative analysis, and document content analysis methods. The urban plans of Lithuanian cities were evaluated according to the following criteria: the time scope and relevance of the plan, the completeness of the analysis of the existing transport system, the assessment of the environment and quality of life in cities, and the compliance of the planned sustainable mobility measures with the needs of the city. The results of the study show that only a portion of Lithuanian cities have prepared sustainable urban mobility plans, and their contents and analytical bases differ. Some of the plans do not provide a sufficiently detailed and relevant analysis of the current situation; therefore, the need for the selected measures is not always clearly justified. The cities analysed generally envisage or apply measures to improve public transport, develop pedestrian and bicycle infrastructure, regulate traffic, create electric vehicle infrastructure, and promote multimodality. It was concluded that sustainable mobility planning in Lithuanian cities is uneven, and its assessment depends not only on the diversity of the envisaged measures but also on the analytical quality of planning documents, the justification of measures, and the consistency of envisaged implementation measures. The study highlights the need to strengthen data-based sustainable mobility planning and to more clearly link the measures envisaged in the plans with the specific challenges of urban transport systems. Full article
(This article belongs to the Special Issue Moving Towards Sustainable Transport in Urban Environments)
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27 pages, 3060 KB  
Review
Upcycling Spent Coffee Grounds: Approaches, Emerging Concepts and Applications
by Sreehitha Pilli, Jeyan Arthur Moses, Senthilkumar Thiruppathi, Sinija Vadakkepulppara Ramachandran Nair and Loganathan Manickam
Foods 2026, 15(12), 2155; https://doi.org/10.3390/foods15122155 (registering DOI) - 15 Jun 2026
Abstract
Spent coffee grounds (SCG) are generated in millions of tonnes annually due to rising global coffee consumption, posing significant challenges, including greenhouse gas emissions, waste-disposal problems, and the loss of valuable compounds like caffeine, dietary fibre, phenolics, antioxidants, proteins, and lipids, offering prospects [...] Read more.
Spent coffee grounds (SCG) are generated in millions of tonnes annually due to rising global coffee consumption, posing significant challenges, including greenhouse gas emissions, waste-disposal problems, and the loss of valuable compounds like caffeine, dietary fibre, phenolics, antioxidants, proteins, and lipids, offering prospects for potential valorization. Its composition is influenced by several factors. This review focuses on recent advancements in the valorization of SCG across sectors such as food, nutraceuticals, bioenergy, and packaging. The emphasis is on pretreatment, extraction, and bioconversion methods, as well as current research gaps, limitations, and future directions. SCG valorization is oriented toward integrated, multi-product biorefinery systems based on green extraction and bioconversion technologies to recover high-value compounds in both the food and non-food sectors. Nonetheless, industrial scalability is limited by composition variability, energy-intensive processing, techno-economic constraints, and safety and regulatory issues that remain unresolved. The shortcomings, such as inadequate standardized characterization, toxicological validation, and pilot-scale studies, are critical gaps. Scalable, energy-efficient processes, AI-assisted optimization, and regulatory alignment development should be a priority in future research, so that sustainable and commercial deployment is possible. Full article
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12 pages, 1462 KB  
Article
Application of SilicoDArT Markers for the Analysis of Genetic Diversity and Population Structure in Moringa oleifera Lam. Cultivated in Mexico
by Rafael Ruiz-Hernández, Martha Hernández-Rodríguez, Arturo Pérez-Vázquez, Emmanuel de Jesús Ramírez-Rivera, Gustavo López-Romero, José Roberto Bautista-Aguilar, Mario Alejandro Hernández-Chontal and Oliver Salas-Valdez
Horticulturae 2026, 12(6), 729; https://doi.org/10.3390/horticulturae12060729 (registering DOI) - 15 Jun 2026
Abstract
The objective of this study was to determine the diversity and genetic structure of M. oleifera populations cultivated in Mexico through SilicoDArT markers. Seeds were collected from 14 populations in various states of the country. The seeds were germinated in greenhouse conditions. DNA [...] Read more.
The objective of this study was to determine the diversity and genetic structure of M. oleifera populations cultivated in Mexico through SilicoDArT markers. Seeds were collected from 14 populations in various states of the country. The seeds were germinated in greenhouse conditions. DNA was extracted from young leaves using a CTAB-based protocol. The extracted DNA was used to genotype each population with DArtseqTM technology. A total of 11,156 SilicoDArT markers were obtained, all of which were polymorphic. On average, the expected heterozygosity of the populations was 0.46, the number of effective alleles was 1.84 and the rareness was 280.53. Principal coordinate analysis and cluster analysis identified three clusters, with no clear association according to cultivation site. Genetic structure analysis determined three original populations (K = 3). Seven populations were assigned to the same ancestral group, six populations exhibited shared ancestries and one population displayed a distinct genetic background, suggesting anthropogenic management and exchange plant material among leaf producers. The identified genetic diversity and population structure constitute a basis for the conservation and management of Mexican moringa germplasm. Full article
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48 pages, 6365 KB  
Article
Physiological Responses of Highbush Blueberry (Vaccinium corymbosum L.) to Combined Water Deficit and Aluminum Stress: The Role of Methyl Jasmonate in Enhancing Stress Resistance
by Jhonny Quintana, Cristina Cáceres, Jéssica Costa, Cledir Santos, Jorge Gonzalez-Villagra, Claudio Inostroza-Blancheteau, Adriano Nunes-Nesi and Marjorie Reyes-Díaz
Horticulturae 2026, 12(6), 728; https://doi.org/10.3390/horticulturae12060728 (registering DOI) - 15 Jun 2026
Abstract
Highbush blueberry (Vaccinium corymbosum L.) is widely cultivated in southern Chile on acidic Andisols, where aluminum (Al3+) toxicity and water deficit frequently occur simultaneously and limit plant performance. However, the integrated physiological responses to these stresses and the potential protective [...] Read more.
Highbush blueberry (Vaccinium corymbosum L.) is widely cultivated in southern Chile on acidic Andisols, where aluminum (Al3+) toxicity and water deficit frequently occur simultaneously and limit plant performance. However, the integrated physiological responses to these stresses and the potential protective role of methyl jasmonate (MeJA) remain poorly understood. This study evaluated the physiological, biochemical, and hormonal responses of two cultivars with contrasting resistance, Legacy (Al-resistant) and Star (Al-sensitive), exposed to Al3+ stress, water deficit, and their combination, with or without MeJA application. Plants were grown in Andisol soil under greenhouse conditions and subjected to eight treatments, with measurements performed at 7, 14, and 21 days. Exposure to stress conditions resulted in decreased growth, reduced leaf water status, diminished photosynthetic performance, lower pigment stability, and decreased auxin concentration as estimated by Salkowski-reactive indolic compounds. Conversely, stress conditions led to increased aluminum (Al) accumulation, elevated proline levels, enhanced lipid peroxidation, and heightened antioxidant responses. Water deficit produced the strongest reductions in photosynthesis, about 48% in Legacy and 65% in Star, whereas Al accumulated mainly in the roots of Star (14-fold). The combined stress intensified physiological limitations and oxidative damage, particularly in the Star cultivar (4-fold), which showed stronger reductions in photosynthetic parameters, higher Al accumulation, and greater lipid peroxidation. In contrast, Legacy maintained more stable physiological performance. Exogenous MeJA mitigated stress effects by reducing Al accumulation (30–35%) and oxidative damage, improving photosynthetic performance (40–60%) and water status, and partially restoring auxin levels and growth in both cultivars, being more evident in the resistant cultivar Legacy. These results indicate that MeJA contributes to the regulation of physiological and antioxidant responses associated with resistance to combined Al toxicity and water deficit in highbush blueberry. Full article
(This article belongs to the Section Biotic and Abiotic Stress)
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37 pages, 14939 KB  
Article
Experimental Assessment and Modeling of Solar Irradiance for an Agrivoltaic Greenhouse for Watermelon Production in Southern Spain
by Anna Kujawa, Natalie Hanrieder, Sergio González Rodríguez, Lyubomir Hristov, Manuel Jesus Blanco, Leontina Berzosa Álvarez, Ana Martínez Gallardo, Adoración Amate González, Marina Casas Fernandez, Francisco Javier Palmero Luque, Manuel López Godoy, María del Carmen Alonso-García, José Antonio Carballo, Luis Fernando Zarzalejo Tirado, Cristina Cornaro and Robert Pitz-Paal
AgriEngineering 2026, 8(6), 245; https://doi.org/10.3390/agriengineering8060245 (registering DOI) - 14 Jun 2026
Abstract
Watermelons account for 7% of the world’s fruit vegetable production. In the European market, Spain contributes around 35% of total watermelon supply, with the majority grown in greenhouses in Almería, Southern Spain. This study presents experimental results from the first agrivoltaic watermelon trial [...] Read more.
Watermelons account for 7% of the world’s fruit vegetable production. In the European market, Spain contributes around 35% of total watermelon supply, with the majority grown in greenhouses in Almería, Southern Spain. This study presents experimental results from the first agrivoltaic watermelon trial conducted in a raspa-y-amagado greenhouse during the 2024 growing season in Almería, Spain. Watermelons were cultivated under two shading treatments with 30% and 50% of the roof area covered with PV modules and compared against an unshaded control group. Throughout the experiment, temperature values in the 30% and 50% zones were 2.2C and 4.3C lower than in the control zone, respectively. The unshaded control zone and the 30% shading treatment maintained DLI conditions within the optimal range between 21/m/day and 32/m/day for most of the crop cycle, while the 50% shading zone remained largely above the minimum threshold of 15/m/day required for adequate crop growth. No statistically significant differences were observed in fruit weight, rind width, fruit firmness, or soluble solids content at harvest. In addition, the experimentally measured irradiance data from this study were compared with simulations from a previously established irradiance model. The model was applied to the raspa-y-amagado greenhouse, and the experimental data were used to perform a long-term comparison between simulated and measured irradiance for 265 days of data. The irradiance model accurately reproduced shading effects from both the PV modules and greenhouse structure, achieving nRMSE values of 0.09, 0.18, and 0.27 for the control, 30% shading, and 50% shading zones, respectively. Full article
23 pages, 3777 KB  
Article
Pre-Treated Gasification Biochar from Tomato Crop Residues as a Component of Soilless Seedling Substrates
by Omer Faruk Tastan, Elif Celik, Murat Dogru, Bahar Yildiz Kutman and Umit Baris Kutman
Horticulturae 2026, 12(6), 727; https://doi.org/10.3390/horticulturae12060727 (registering DOI) - 14 Jun 2026
Abstract
Tomato crop residues (TCR) from soilless greenhouses are treated as waste, causing greenhouse gas emissions and biomass loss. Within a circular economy framework, gasification converts TCR into renewable energy and biochar; however, its high pH and electrical conductivity (EC) limit its use as [...] Read more.
Tomato crop residues (TCR) from soilless greenhouses are treated as waste, causing greenhouse gas emissions and biomass loss. Within a circular economy framework, gasification converts TCR into renewable energy and biochar; however, its high pH and electrical conductivity (EC) limit its use as a substrate. This study evaluated whether pre-treatment could enable TCR biochar to act as a substrate component and nutrient source in tomato and pepper seedlings. Biochar was produced by gasification and pre-treated by water incubation plus nitric acid, reducing EC from 27 to 8.7 dS m−1 and pH from 10.4 to 8.2 while achieving nitrate loading without leaching. Pristine biochar severely restricted growth. Subsequent experiments evaluated pre-treated biochar mixed with perlite or cocopeat, with or without external N and K. The 15/85% (w/w) pre-treated biochar/cocopeat mixture (PTB/C) showed the best overall performance. In the absence of additional N/K, PTB/C produced shoot biomass and shoot N concentrations comparable to N-/K-supplemented cocopeat; shoot K was comparable in tomato and higher in pepper. With N and K supplementation, PTB/C exceeded supplemented cocopeat biomass by 1.41- and 1.95-fold in tomato and pepper, respectively. These results indicate that pre-treated TCR biochar can reduce dependence on imported cocopeat and external N/K supply. Full article
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22 pages, 2917 KB  
Article
Estimation and Trend Analysis of Emissions from Ships Registered in Republic of Korea
by So-Hyun Park, Siljung Yeo, Jae-Hyuk Choi and Won-Ju Lee
Energies 2026, 19(12), 2835; https://doi.org/10.3390/en19122835 (registering DOI) - 14 Jun 2026
Abstract
To address the climate impact of maritime transport, the International Maritime Organization (IMO) has implemented regulations targeting ship emissions, particularly greenhouse gases (GHGs), to achieve net-zero emissions by 2050. Meeting these goals requires accurate estimates of air pollutant emissions and a clear understanding [...] Read more.
To address the climate impact of maritime transport, the International Maritime Organization (IMO) has implemented regulations targeting ship emissions, particularly greenhouse gases (GHGs), to achieve net-zero emissions by 2050. Meeting these goals requires accurate estimates of air pollutant emissions and a clear understanding of emission trends. This study estimated air pollutant emissions from ships registered in Republic of Korea between 2021 and 2023 using a bottom-up approach. The methodology incorporates ship specification data, regression models, and correction factors based on actual fuel consumption. For ships lacking engine power data, power was estimated using a regression of gross tonnage by ship type. Annual fuel consumption was calculated using engine power, fuel type, engine configuration, and ship age, and emission factors were applied to estimate CO2, CH4, N2O, and other air pollutants. The results showed that CO2 accounts for over 98% of GHG emissions, while cargo ships, which represent only 10% of the fleet, contribute more than 60% of total GHG emissions. These findings highlight the importance of prioritizing cargo vessels in CO2 reduction strategies. This study provides baseline data to align policy development with IMO regulations and underscores the need for a continuous national framework for estimating ship emissions. Full article
(This article belongs to the Special Issue Sustainable Combustion Technologies for the Energy Transition)
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12 pages, 6108 KB  
Article
Ultra-Early OCT Changes After Intravitreal Injection: Evidence Consistent with Transient Mechanical Compression
by Yehya Tlaiss, John Warrak and Elias Warrak
Vision 2026, 10(2), 35; https://doi.org/10.3390/vision10020035 (registering DOI) - 14 Jun 2026
Abstract
(1) Background: Ultra-early optical coherence tomography (OCT) changes following intravitreal injection may reflect transient mechanical compression rather than pharmacologic effects; however, this temporal profile has not been rigorously characterised with appropriate statistical methodology. (2) Methods: In this prospective observational study, 40 eyes of [...] Read more.
(1) Background: Ultra-early optical coherence tomography (OCT) changes following intravitreal injection may reflect transient mechanical compression rather than pharmacologic effects; however, this temporal profile has not been rigorously characterised with appropriate statistical methodology. (2) Methods: In this prospective observational study, 40 eyes of 40 consecutive patients (one per patient) with macular edema secondary to neovascular age-related macular degeneration (nAMD), diabetic macular edema (DME), or chronic central serous retinopathy (CSR) underwent intravitreal bevacizumab (n = 35) or triamcinolone acetonide (n = 5). Goldmann applanation tonometry and spectral-domain OCT were performed at baseline, 2–5 min, 15 ± 5 min, 24 h, and 48 h post-injection. Repeated-measures ANOVA with Greenhouse–Geisser correction, linear regression, and Spearman rank correlation were applied. (3) Results: Central subfield thickness (CST) decreased markedly at 15 ± 5 min (mean −24.8 ± 11.5%; 95% CI: −28.5% to −21.1%; p < 0.001; partial η2 = 0.70), with near-complete rebound by 48 h (−1.0%; p = 0.400). Peak intraocular pressure (IOP) elevation correlated with CST reduction (Spearman rs = 0.61; 95% CI: 0.39–0.77; p < 0.001), and baseline CST predicted thinning magnitude (R2 = 0.52; p < 0.001). (4) Conclusions: Ultra-early OCT thinning after intravitreal injection is consistent with transient mechanical compression. Retinal thickness measurements within 48 h post-injection should be interpreted with caution when assessing treatment response, as early anatomic reduction may not reflect pharmacologic efficacy. Full article
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62 pages, 4424 KB  
Review
The Mediterranean Diet as a Sustainable Dietary Pattern: A State-of-the-Art Narrative Review of Health, Environmental and Socioeconomic Dimensions
by Georgios K. Vasios, Maria Gialeli, Georgios Antasouras and Constantinos Giaginis
Nutrients 2026, 18(12), 1925; https://doi.org/10.3390/nu18121925 (registering DOI) - 13 Jun 2026
Abstract
Background/Objectives: The increasing burden of non-communicable diseases, together with accelerating environmental degradation, highlights the urgent need for sustainable dietary patterns that promote both human and planetary health. The Mediterranean diet (MedDiet), traditionally followed in countries bordering the Mediterranean basin, has gained recognition as [...] Read more.
Background/Objectives: The increasing burden of non-communicable diseases, together with accelerating environmental degradation, highlights the urgent need for sustainable dietary patterns that promote both human and planetary health. The Mediterranean diet (MedDiet), traditionally followed in countries bordering the Mediterranean basin, has gained recognition as a model of sustainable nutrition due to its well-documented health benefits and relatively low environmental impact. However, its broader role within sustainable food systems requires comprehensive and interdisciplinary evaluation. The aim of this review is to provide a state-of-the-art synthesis of the evidence on the MedDiet as a sustainable dietary pattern, integrating its health, environmental, economic, and socio-cultural dimensions. Methods: This state-of-the-art narrative review synthesizes evidence from peer-reviewed literature on the MedDiet and sustainability. Relevant studies were identified through major scientific databases, focusing on publications addressing nutritional, environmental, economic, and socio-cultural dimensions. Both observational and interventional studies, as well as modeling and life cycle assessment analyses, were included. Additional sources from international organizations and policy reports were incorporated to contextualize global trends and challenges. Results: High adherence to the MedDiet is consistently associated with a reduced risk of cardiovascular disease, type 2 diabetes, cancer, and all-cause mortality. From an environmental perspective, the MedDiet is associated with lower greenhouse gas emissions, reduced land and water use, and enhanced biodiversity conservation compared with Western dietary patterns. Economically, it may represent a cost-effective dietary model and support local food systems when grounded in traditional practices, although affordability varies across contexts. Socio-culturally, the MedDiet promotes food heritage, culinary skills, and social cohesion. Nevertheless, globalization, urbanization, and the increasing consumption of ultra-processed foods have contributed to declining adherence, posing significant challenges to its sustainability and scalability. Moreover, the sustainability benefits of the MedDiet seem to be context-dependent rather than intrinsic, raising several challenges and limitations for its adoption. Conclusions: The MedDiet should be viewed not as a definitive solution to global food-system challenges but as a valuable reference model that illustrates how dietary practices can contribute simultaneously to human health, environmental sustainability, and cultural continuity. Modern sustainable dietary strategies should build upon the strengths of the MedDiet while recognizing its limitations, embracing contextual adaptation, and addressing the structural determinants that shape food choices. Full article
(This article belongs to the Section Nutritional Policies and Education for Health Promotion)
23 pages, 1272 KB  
Article
Dynamic Optimization of Incoming Quality Control Policies for Cost, Carbon, and Energy Reduction Using Bayesian Reinforcement Learning
by David Massetti, Mehdi Raoofi, Tiziano Miroglio, Marco Mosca and Flavio Tonelli
Sustainability 2026, 18(12), 6094; https://doi.org/10.3390/su18126094 (registering DOI) - 13 Jun 2026
Abstract
The transition towards sustainable manufacturing necessitates complex optimization that integrates economic goals with environmental factors, such as energy consumption and greenhouse gas emissions. This research addresses the critical challenge of optimizing the Incoming Quality Control (IQC) policy for raw material batches. The primary [...] Read more.
The transition towards sustainable manufacturing necessitates complex optimization that integrates economic goals with environmental factors, such as energy consumption and greenhouse gas emissions. This research addresses the critical challenge of optimizing the Incoming Quality Control (IQC) policy for raw material batches. The primary objective is formulated as a multi-criteria control problem that jointly minimizes the weekly final product cost, carbon footprint, and energy consumption. To handle sequential decision making under uncertainty, we adopt a scalarized reinforcement learning (RL) reward that combines these objectives into a single value function and explores different trade-offs through alternative weight configurations. To effectively handle the uncertainty in incoming quality and the sequential decision making required for dynamic control, the optimization problem is modeled as a Bayesian Adaptive Markov Decision Process (BAMDP). To maintain computational tractability despite the continuous belief space inherent in the BAMDP formulation, we employ a Deep Q-Network (DQN) architecture acting as an approximate dynamic programming solver. The Bayesian framework represents model uncertainty explicitly, updates beliefs as new inspection evidence becomes available, and allows prior domain knowledge on supplier quality to be incorporated into the learning process. The BAMDP formulation is used to learn a set of adaptive inspection policies that adjust the IQC strategy over time to achieve conflicting goals: reducing inspection costs while maintaining standard quality, minimizing energy consumption, and lowering CO2-equivalent emissions. The goal is to find robust policies that balance these trade-offs under different quality and demand conditions. This methodology aligns with the principles of Industry 5.0 by leveraging advanced artificial intelligence (AI) methods, such as reinforcement learning (RL), coupled with a stochastic simulation of the production system, based on a geometric/physical model of the component’s tolerance chains, to support decision-makers in designing and assessing sustainable IQC strategies. Comparative simulations on the case study, including a benchmark against ISO 2859-1 sampling plans, confirm that this dynamic and risk-aware optimization paradigm can reduce overall cost, energy use, and environmental impact across various quality conditions, while preserving outgoing quality. Full article
25 pages, 2872 KB  
Article
Using Machine Learning Algorithms to Evaluate the TVPD Evapotranspiration Prediction Model for Use in Irrigation Management
by Ronnie J. Dunn, Hannah Kinmonth-Schultz and Michael P. Nattrass
Agriculture 2026, 16(12), 1307; https://doi.org/10.3390/agriculture16121307 (registering DOI) - 12 Jun 2026
Viewed by 206
Abstract
In the future, agriculture will need better irrigation management options to produce more food and decrease its air and water pollution contributions. Hydroponic systems conserve water over field production, but up to 50% of applied irrigation could be discharged from open-drain systems. TVPD [...] Read more.
In the future, agriculture will need better irrigation management options to produce more food and decrease its air and water pollution contributions. Hydroponic systems conserve water over field production, but up to 50% of applied irrigation could be discharged from open-drain systems. TVPD is an evapotranspiration model developed for greenhouse production, particularly for hydroponics. In this study, we calibrate and evaluate TVPD on environmental and evapotranspiration data from hydroponic tomato production and compare predictions to those of random forest (RF) and K-nearest neighbors (KNN). Using five time-ordered data splits, we sought to gauge prediction accuracy for data-limited settings, where the model needs to be implemented with the least calibration time possible, and we evaluated TVPD, RF, and KNN with a 10-fold cross-validation to assess overall model robustness. Across the five data splits, TVPD produced more accurate predictions (r2: 0.86 to 0.90; RMSE: 0.1739 to 0.5796 L tray−1) than RF (r2: 0.06 to 0.73; RMSE: 0.7354 to 2.0505 L tray−1) and KNN (r2: 0.06 to 0.59; RMSE: 0.7694 to 1.7090 L tray−1). With calibration on only the first five days of data, TVPD was able to produce acceptable predictions (r2 = 0.87, RMSE = 0.5796 L tray−1). The mean r2 for a 10-fold cross-validation was 0.81 for TVPD, 0.88 for RF and 0.81 for KNN, and mean RMSE values were slightly better for the cross-validation for RF (0.4970 L tray−1) and KNN (0.4968 L tray−1) than for TVPD (0.5922 L tray−1). Overall, TVPD could be a useful model to predict evapotranspiration for irrigation management and could decrease the volume of discharged hydroponic waste solution. Full article
(This article belongs to the Special Issue Precision Irrigation System: Challenges and Opportunities)
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16 pages, 2329 KB  
Article
Enhancing Virulence of an Entomopathogenic Fungus Against the Diamondback Moth, Plutella xylostella (Lepidoptera: Plutellidae), Through Integrated Formulation Strategies Under Laboratory and Greenhouse Conditions
by Muhammad Riaz, Tsui-Ying Chang, Lekhnath Kafle and Wen-Hua Chen
Insects 2026, 17(6), 622; https://doi.org/10.3390/insects17060622 (registering DOI) - 12 Jun 2026
Viewed by 108
Abstract
Plutella xylostella (Linnaeus) (Lepidoptera: Plutellidae) is a major cruciferous crop pest worldwide with resistance to multiple insecticide classes, highlighting the need for sustainable alternatives. Entomopathogenic fungi (EPF) are promising biocontrol agents, but their efficacy is limited by slow pathogenicity, environmental sensitivity, and low [...] Read more.
Plutella xylostella (Linnaeus) (Lepidoptera: Plutellidae) is a major cruciferous crop pest worldwide with resistance to multiple insecticide classes, highlighting the need for sustainable alternatives. Entomopathogenic fungi (EPF) are promising biocontrol agents, but their efficacy is limited by slow pathogenicity, environmental sensitivity, and low persistence on insect cuticles. This study evaluated integrated formulation strategies to enhance the virulence of Beauveria namnaoensis PM-02 against P. xylostella under laboratory and greenhouse conditions. Putative copper and zinc nanoparticle preparations were generated using fungal biomass extracts, with nanoparticle formation inferred from visual changes in the reaction mixtures. Oil-emulsified fungal formulations and combinations with emamectin benzoate were also evaluated. Larval mortality increased significantly with concentration, indicating a clear dose-dependent response. The combined treatment of oil-emulsified fungus and emamectin benzoate, along with emamectin benzoate alone, resulted in the highest larval mortality (100%), whereas fungus alone caused the lowest mortality (43.3%). Lethal concentration (LC50) analysis indicated high toxicity of the combined treatment, while lethal time (LT50) values demonstrated more rapid mortality for emamectin benzoate (0.176 days) and the combined treatment (0.830 days) compared with fungus alone (6.25 days). Under greenhouse conditions, the combined treatment showed the highest efficacy, reducing larval survival to 30% and demonstrating enhanced insecticidal activity. Overall, integrated formulation strategies significantly improved fungal efficacy against P. xylostella. Full article
(This article belongs to the Section Insect Pest and Vector Management)
16 pages, 2956 KB  
Article
Post-Harvest and Frying Quality of Potato Grown Using Different Planting Methods and Crop Conditions
by Javier Giovanni Álvarez-Herrera, Marilcen Jaime-Guerrero and Juan Diego Becerra-Lagos
Crops 2026, 6(3), 55; https://doi.org/10.3390/crops6030055 - 12 Jun 2026
Viewed by 69
Abstract
Potatoes are a crop of great importance for global food security, and their industrialization requires certain postharvest quality characteristics that are affected by cultivation practices. Unlike previous studies that focused on single agronomic factors or genotype effects, to increase knowledge, this work evaluates [...] Read more.
Potatoes are a crop of great importance for global food security, and their industrialization requires certain postharvest quality characteristics that are affected by cultivation practices. Unlike previous studies that focused on single agronomic factors or genotype effects, to increase knowledge, this work evaluates the interaction between planting method (bag vs. soil) and cultivation condition (greenhouse vs. open field) on postharvest and frying quality of the high-altitude variety ‘Diacol Capiro’. A completely randomized design was used with four treatments arranged in a 2 × 2 factorial layout, where the first factor was the planting method (in bags or in soil) and the second factor was the cultivation conditions (in a greenhouse or in an open field). Tubers grown in a greenhouse, especially with planting in bags, showed greater starch retention, higher firmness, lower soluble solids content, and less mass loss during storage. The starch content varied significantly among treatments, reaching a maximum of 6.9% after 35 days of storage. The specific gravity of the fried potatoes was higher in greenhouse-grown tubers (1.080) than in those planted in the open field (1.070), with values close to the industrial standard (>1.080). The skin luminosity decreased by 16.2% during storage, while the b* parameter of the flesh (yellow color) was higher in tubers from greenhouse planting. Overall, ‘Diacol Capiro’ tubers grown in a greenhouse with planting in bags showed better postharvest attributes and greater potential for frying quality. Full article
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Article
Cultivation Mode Reshapes Root Fungal Endophyte Communities in Dendrobium officinale (Orchidaceae)
by Junxi Cheng, Yong Chen, Jiayu Dong, Jianhao Jiang, Chuyan Fu, Xingze Huang, Jianhong Zhou, Chao Jiang, Xiaofeng Wang and Li Liang
Diversity 2026, 18(6), 359; https://doi.org/10.3390/d18060359 - 12 Jun 2026
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Abstract
Background: Symbiotic fungi play essential roles throughout the entire cycle of orchid plants, including seed germination, seedling development, and maturation. Dendrobium officinale Kimura & Migo (Orchidaceae) (D. officinale) is a rare and highly valued traditional Chinese medicinal herb. Currently, artificial breeding [...] Read more.
Background: Symbiotic fungi play essential roles throughout the entire cycle of orchid plants, including seed germination, seedling development, and maturation. Dendrobium officinale Kimura & Migo (Orchidaceae) (D. officinale) is a rare and highly valued traditional Chinese medicinal herb. Currently, artificial breeding using tissue culture technology is widely adopted and essential in the Dendrobium industry; however, this approach may impair or disrupt the plant’s ability to establish and maintain symbiotic relationships with mycorrhizal fungi. Methods: In this study, the fungal endophyte community (FEC) in the roots of D. officinale cultivated under four different modes was analyzed using high-throughput sequencing. Correlation analyses were also carried out to examine the relationships between bioactive compounds and the FEC. Results: (1) The FEC in D. officinale roots was dominated by Ascomycota and Basidiomycota, with significant differences in abundance, diversity, and community structure among cultivation modes; (2) the FEC under greenhouse cultivation differed significantly from those under tree epiphytic cultivation in terms of fungal nutritional types and dominant taxa; (3) six major mycorrhizal fungal taxa were identified in Dendrobium roots, although non-mycorrhizal fungi accounted for approximately 97% of the community; and (4) polysaccharide content in Dendrobium stems was positively correlated with certain root fugal endophytes (Exophiala, alaromyces, Pseudodactylaria, and Fellomyces). Conclusions: This study provides a foundation for understating the growth of D. officinale under different cultivation modes and highlights the relationship between bioactive compound accumulation and fungal endophyte communities. Full article
(This article belongs to the Section Microbial Diversity and Culture Collections)
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