Search Results (356)

Facial expression plays an important role in human–computer interaction and affective computing. However, existing expression recognition methods cannot effectively capture multi-scale structural details contained in facial expressions, leading to a decline in recognition accuracy. Inspired by the multi-scale processing mechanism of the biological visual system, this paper proposes a wavelet-guided global–local feature aggregation network (WGGLFA) for facial expression recognition (FER). Our WGGLFA network consists of three main modules: the scale-aware expansion (SAE) module, which combines dilated convolution and wavelet transform to capture multi-scale contextual features; the structured local feature aggregation (SLFA) module based on facial keypoints to extract structured local features; and the expression-guided region refinement (ExGR) module, which enhances features from high-response expression areas to improve the collaborative modeling between local details and key expression regions. All three modules utilize the spatial frequency locality of the wavelet transform to achieve high-/low-frequency feature separation, thereby enhancing fine-grained expression representation under frequency domain guidance. Experimental results show that our WGGLFA achieves accuracies of 90.32%, 91.24%, and 71.90% on the RAF-DB, FERPlus, and FED-RO datasets, respectively, demonstrating that our WGGLFA is effective and has more capability of robustness and generalization than state-of-the-art (SOTA) expression recognition methods. Full article

The salinization of agricultural soils is a serious threat to farming and ecological balance in arid and semi-arid regions. Accurate estimation of soil water-soluble ions (calcium, carbonate, magnesium, and sulfate) is necessary for correct monitoring of soil salinization and sustainable land management. Hyperspectral ground-based data are valuable in soil salinization monitoring, but the acquisition cost is high, and the coverage is small. Therefore, this study proposes a two-stage deep learning framework with multispectral remote-sensing images. First, the wavelet transform is used to enhance the Transformer and extract fine-grained spectral features to reconstruct the ground-based hyperspectral data. A comparison of ground-based hyperspectral data shows that the reconstructed spectra match the measured data in the 450–998 nm range, with R² up to 0.98 and MSE = 0.31. This high similarity compensates for the low spectral resolution and weak feature expression of multispectral remote-sensing data. Subsequently, this enhanced spectral information was integrated and fed into a novel multiscale self-attentive Transformer model (MSATransformer) to invert four water-soluble ions. Compared with BPANN, MLP, and the standard Transformer model, our model remains robust across different spectra, achieving an R² of up to 0.95 and reducing the average relative error by more than 30%. Among them, for the strongly responsive ions magnesium and sulfate, R² reaches 0.92 and 0.95 (with RMSE of 0.13 and 0.29 g/kg, respectively). For the weakly responsive ions calcium and carbonate, R² stays above 0.80 (RMSE is below 0.40 g/kg). The MSATransformer framework provides a low-cost and high-accuracy solution to monitor soil salinization at large scales and supports precision farmland management. Full article

Drought impacts agricultural production and regional sustainable development. Accordingly, timely and accurate drought monitoring is essential for ensuring food security in rain-fed agricultural regions. Alternating drought and flood events frequently occur in the Heilongjiang River Basin, the largest grain-producing area in Far East Asia. However, spatiotemporal variability in drought is not well understood, in part owing to the limitations of the traditional Temperature Vegetation Dryness Index (TVDI). In this study, an Improved Temperature Vegetation Dryness Index (ITVDI) was developed by incorporating Digital Elevation Model data to correct land surface temperatures and introducing a constraint line method to replace the traditional linear regression for fitting dry–wet boundaries. Based on MODIS (Moderate-resolution Imaging Spectroradiometer) normalized vegetation index and land surface temperature products, the Heilongjiang River Basin, a cross-border basin between China, Mongolia, and Russia, exhibited pronounced spatiotemporal variability in drought conditions of the growing season from 2001 to 2023. Drought severity demonstrated clear geographical zonation, with a higher intensity in the western region and lower intensity in the eastern region. The Mongolian Plateau and grasslands were identified as drought hotspots. The Far East Asia forest belt was relatively humid, with an overall lower drought risk. The central region exhibited variation in drought characteristics. From the perspective of cross-national differences, the drought severity distribution in Northeast China and Inner Mongolia exhibits marked spatial heterogeneity. In Mongolia, regional drought levels exhibited a notable trend toward homogenization, with a higher proportion of extreme drought than in other areas. The overall drought risk in the Russian part of the basin was relatively low. A trend analysis indicated a general pattern of drought alleviation in western regions and intensification in eastern areas. Most regions showed relatively stable patterns, with few areas exhibiting significant changes, mainly surrounding cities such as Qiqihar, Daqing, Harbin, Changchun, and Amur Oblast. Regions with aggravation accounted for 52.29% of the total study area, while regions showing slight alleviation account for 35.58%. This study provides a scientific basis and data infrastructure for drought monitoring in transboundary watersheds and for ensuring agricultural production security. Full article

Fermented feed has considerable potential as a high-quality protein source in animal production. This research aimed to examine the effects of a low-protein (LP) diet, supplemented with fermented feed, on the meat quality and intestinal health of growing–fattening pigs. The pigs were randomly divided into three groups, and a total of 72 growing–fattening pigs were subjected to the experiment. They were fed the control (CON) diet, LP diet, and LP diet supplemented with fermented rapeseed meals and fermented distiller’s grains (FLP), respectively. The results indicated that the FLP diet altered the structure of the intestinal microbiota and regulated the composition of unsaturated fatty acids in the longissimus dorsi. Furthermore, the FLP diet upregulated the expression of genes associated with myosin heavy chain isoforms (p < 0.05) and modified the content of short-chain fatty acids in the intestines (p < 0.05). In summary, the addition of fermented distiller’s grains (FDGs) and fermented rapeseed meals (FRMs) to the LP diet enhanced fatty acid metabolism and intestinal barrier function in growing–fattening pigs. Full article

The objective of this work was to explore the effect of taxonomically and phytochemically rich swards, as opposed to ‘status quo’ monoculture of ryegrass and white clover swards, on animals and milk, by assessing the metabolomic profile of plant and milk samples. The results of this study suggest that metabolomic profiles and metabolism are altered by dietary diversity and grazing management. Several metabolites associated with enhanced consumer health were elevated in milk from cows that were grazed in functionally diverse swards as opposed to monoculture of ryegrass and white clover swards. The results show the value of metabolomics for the identification of metabolites associated with a ‘pasture-fed diet’, which is considered beneficial to human health compared with grain-fed milk products. Metabolomics also provide evidence that grazing management and the presence of key forages may be more beneficial for market differentiation of milk products that can enhance consumer health than maximisation of sward species diversification. Full article

One of the most effective methods of improving the properties of aluminium alloys is grain refining using Al-Ti-B master alloys. In contrast, zirconium is a key alloying element, used mainly in 2xxx and 7xxx series aluminium alloys, where it contributes to dispersion enhancement and reduces the rate of dynamic recrystallisation. However, even trace amounts of zirconium—just a few hundredths of ppm—significantly reduce the performance of Al-Ti-B grain refiners, a phenomenon known as ‘Zr poisoning’. This study investigates the impact of holding time and the level of Al-5Ti-1B addition on the microstructure and properties of an AlMgSi(Cu) alloy containing 0.15 wt.% Zr, cast as 7-inch DC billets. The structure and phase distribution were characterised using optical microscopy (OM), scanning electron microscopy (SEM) with energy-dispersive spectroscopy (EDS), and transmission electron microscopy (TEM). Grain size and morphology were evaluated through macrostructure analysis (etched cross-sections and polarised light microscopy), while chemical and elemental distributions were analysed via SEM-EDS and STEM-EDS mapping. Additionally, Brinell hardness measurements were conducted across the billet diameter to assess the correlation between grain size and mechanical properties. The results show that reducing holding time and increasing the Al-5Ti-1B addition improves grain refinement efficiency despite the presence of Zr. The finest grain structure (150–170 μm) and most homogeneous hardness distribution were achieved when the grain refiner was continuously fed during casting at 80 ppm B. These findings are supported by the literature and contribute to a deeper understanding of the Zr poisoning effect and its mitigation through optimized casting practice. Full article

The growing demand for biofuels, especially ethanol produced from corn, has driven the production of co-products such as dried distillers grains with solubles (DDGS). With a high protein content (around 30%), fiber, and minerals, DDGS presents an economical alternative for animal nutrition, replacing traditional sources like soybean meal while maintaining productive performance and reducing costs. This study evaluated the total replacement of soybean meal with DDGS in the diet of confined Holstein cattle, focusing on weight gain, feed intake, digestibility, feed efficiency, animal health, meat quality, and economic viability. The 24 animals received diets with 80% concentrate, containing either DDGS or soybean meal, and no significant differences were observed in terms of body weight (p = 0.92), feed intake (p = 0.98), or feed efficiency (p = 0.97) between the two treatments. The average daily gain was 1.25 and 1.28 kg for cattle in the DDGS and soybean meal groups, respectively (p = 0.92). Regarding metabolic and digestive parameters, no relevant changes were found in blood levels, except for higher serum cholesterol (p = 0.03) levels in animals fed DDGS. The digestibility of neutral detergent fiber (NDF) (p = 0.03) and acid detergent fiber (ADF) (p = 0.05) was lower in the DDGS group, while the digestibility of ether extract was higher (p = 0.02). Rumen fluid analysis revealed an increase in the production of short-chain fatty acids (p = 0.01), such as acetic and butyric acids (p = 0.01), in the DDG-fed animals. In terms of meat quality, animals fed DDGS produced meat with lower levels of saturated fatty acids (SFA) (p = 0.05) and higher levels of unsaturated fatty acids (UFA) (p = 0.02), especially oleic acid (p = 0.05). This resulted in a healthier lipid profile, with a higher UFA/SFA ratio (p = 0.01). In terms of economic viability, DDGS-based diets were 10.5% cheaper, reducing the cost of production per animal by 7.67%. Profitability increased by 110% with DDGS compared to soybean meal, despite the high transportation costs. Therefore, replacing soybean meal with DDGS is an efficient and economical alternative for feeding confined cattle, maintaining zootechnical performance, increasing meat lipid content and improving fatty acid profile, and promoting higher profitability. This alternative is particularly advantageous in regions with easy access to the product. Full article

Real-time information on key state variables during fermentation is crucial for the effective optimization and control of bioprocesses. Specialized sensors for online or at-line monitoring of these variables are often associated with high costs, especially during early-stage process optimization. In this study, fed-batch processes of an L-phenylalanine (L-phe) production process were carried out using a recombinant Escherichia coli strain under varying inducer concentrations. The available online process variables from the L-phe production process were used to estimate the state variables biomass, glycerol, L-phe, acetate, and L-tyrosine (L-tyr) via partial least-squares regression (PLSR). These predictions were then incorporated as measurements into an unscented Kalman filter (UKF). The filter uses a coarse-grained model as a state estimator, which, in addition to extracellular variables, also provides information on intracellular states. The results of PLSR showed very good prediction accuracy for L-phe, moderate accuracy for glycerol, biomass, and L-tyr and poor performance for acetate concentrations. In combination with the UKF, the estimation of the L-phe concentrations was greatly improved compared to the CGM, whereas further improvement is still needed for the remaining state variables. Full article

Dietary modification plays a crucial role in managing and preventing hyperlipidemia. This study examined the combination of highland barley, tartary buckwheat, mung beans, Ormosia hosiei, black rice, and corn germ oil in multi-grain biscuit form. This formulation leverages the synergistic interactions among bioactive compounds, which exert preventive and therapeutic effects against lipid disorders. C57BL/6N mice were fed a high-fat diet for 12 weeks to establish a hyperlipidemia model, followed by feeding with highland barley tartary buckwheat coarse-grain biscuits for 4 weeks. The experimental outcomes revealed that the highland barley tartary buckwheat coarse-grain biscuits effectively controlled body weight and reduced fasting blood sugar levels: body weight was restored to approximately 29 g, and the fasting blood sugar level returned to the normal range of 6 mmol/L. We also observed improved organ indices and regulated blood lipids in hyperlipidemic mice. The total cholesterol of high-fat mice was reduced to 5 mmol/L and the triglyceride level to 1 mmol/L. A significant reduction in inflammatory markers and histopathological improvement in hepatic and adipose tissues were also observed. The intervention enhanced leptin and adiponectin secretion while elevating concentrations of acetic, propionic, butyric, valeric, and caproic acids. Microbiome analysis demonstrated favorable shifts in bacterial populations, characterized by increased Bacteroidetes and Verrucomicrobia abundance and a decreased Firmicutes-to-Proteobacteria ratio, promoting beneficial genera while suppressing potentially pathogenic taxa. These findings suggest that the developed highland barley tartary buckwheat coarse-grain biscuits are a promising dietary intervention for hyperlipidemia management. The effects were potentially mediated through gut microbiota modulation and enhanced short-chain fatty acid production. This research provides novel insights into functional food development for hyperlipidemia. Full article

Disbudding is a husbandry practice that causes pain and discomfort to calves. As a prominent welfare concern, it is now standard practice for calves to be given analgesic treatment such as a nonsteroidal anti-inflammatory drug (NSAID) injection. Meloxicam is a commonly used NSAID as it provides pain relief for up to 44 h following disbudding. However, since symptoms can persist for up to two weeks, it was hypothesised that more prolonged analgesic treatment would promote better welfare outcomes than the conventional injection. This study tested a novel treatment whereby disbudded calves were fed grain-based pellets medicated with meloxicam over a 7-day period. Lower levels of horn site inflammation were observed for the pellet treatment across the 7-day feeding period in comparison to the conventional injection. The pellet treatment calves also exhibited less pain-specific and more positive social-specific behaviours during and beyond the feeding period. Together, these results suggest that lower levels of inflammation enacted by prolonged meloxicam administration have an active role in reducing pain and maintaining the affectivity of disbudded calves. With the goal of establishing sustained disbudding treatment as a new industry standard, future research will focus on larger-scale results reproducibility and maximising treatment practicality. Full article

Brewer’s spent grain (BSG), the primary byproduct generated by the brewing industry, holds significant potential as an ingredient in aquafeeds. However, its high content of non-starch polysaccharides (NSP) restricts the amount that can be incorporated into fish diets. To address these limitations, various pretreatment methods (physical, chemical, or enzymatic) can be applied prior to its inclusion in feed formulations. The objectives of the present study were (i) to optimize the conditions for enzymatic or microwave pretreatments to enhance the nutritional and functional profile of BSG, (ii) to determine the bioaccessibility and availability of specific nutrients and antioxidant compounds in diets including a high amount of BSG (pretreated or not) using an in vitro assay simulating the digestion of the gilthead seabream (Sparus aurata), and (iii) to evaluate the effect of such diet pretreatments on growth, metabolism, intestinal microbiota, and oxidative status in live fish. For this study, three experimental diets were formulated: one containing 20% untreated BSG (C) and two containing the same amount of BSG pretreated either enzymatically (H) or with microwave heating (MW). Each diet was administered ad libitum to triplicate groups of 22 juvenile sea bream (mean weight 60 g) over a period of three months. The results indicated that the microwave-treated BSG led to improved growth (0.69 ± 0.02%) and feed efficiency (0.80 ± 0.02 weight gain/total feed intake) and enhanced immune status (alkaline phosphatase activity = 11,811.68 ± 3426.92 U/mg SP), compared to the control diet (SGR = 0.59 ± 0.06%; FE: 0.68 ± 0.03 weight gain/total feed intake; alkaline phosphatase activity = 8590.29 ± 3663.44 U/mg SP). Moreover, fish fed on both pretreated BSG diets exhibited significant differences in metabolic parameters and functional profile of their intestinal microbiota when compared to the control group. Consequently, the findings suggest that the pretreatment of BSG, whether by enzymatic or microwave methods, results in notable differences in its nutritional value and the bioavailability of functional components, which, in turn, have a substantial impact on the growth and metabolism of gilthead seabream. Full article

This study explored the impact of licorice stem and leaf forage in the diet of meat sheep on their growth performance, physiological parameters, immunity, and gut microbiota. Conducted in the Hotan region of Xinjiang, the experiment involved 40 healthy local Hotan sheep (5–6 months old) that were randomly assigned to four groups, with both corn stalks and corn grain partially substituted at varying levels (30%, 40%, and 50%) by licorice stems and leaves for 80 days. Daily feed intake and refusal were recorded to assess feeding behavior and efficiency, while body weight was measured every 20 days to monitor growth performance. On day 80, blood samples were collected via venipuncture for biochemical and immune function analysis, followed by slaughter to obtain meat samples for quality assessment. Results showed that dietary licorice supplementation significantly improved growth performance. Sheep in the 40% replacement group had the highest final body weight and average daily gain (p < 0.05), indicating better feed efficiency. These findings suggest that replacing corn stalks and grain with licorice stems and leaves at a 40% ratio can significantly improve growth performance. Biochemical results showed improved protein metabolism and lipid profiles, with increased TP and ALB and decreased TC and TG levels. Immune indicators, including IgG, IgM, IgA, and IL-6, were also elevated in licorice-fed groups. In summary, licorice stems and leaves demonstrated clear nutritional and immunological benefits, especially at the 40% inclusion level. Gut microbiota analysis showed that licorice stems and leaves increased the relative abundance of Firmicutes while reducing Bacteroidota. At the genus level, UCG_005 and Bacteroides increased with higher licorice concentrations, whereas Treponema decreased. Pearson correlation analysis indicated that UCG_005 was positively correlated with TP, ALB, IgM, and IL-6 and negatively correlated with TG and TNF-α. These findings suggest that using licorice stems and leaves as a replacement for both corn stalks and corn grain modulates gut microbiota composition and is closely associated with immune and biochemical responses in sheep. Full article

The Hetao Plain Irrigation District of Inner Mongolia faces critical agricultural sustainability challenges due to its arid climate, exacerbated by tightening Yellow River water allocations and pervasive water inefficiencies in the current wheat cultivation practices. This study addresses water scarcity by evaluating the impact of regulated deficit irrigation strategies on spring wheat production, with the dual objectives of enhancing water conservation and optimizing yield–quality synergies. Through a two-year field experiment (2020~2021), four irrigation regimes were implemented: rain-fed control (W0), single irrigation at the tillering–jointing stage (W1), dual irrigation at the tillering–jointing and heading–flowering stages (W2), and triple irrigation incorporating the grain-filling stage (W3). A comprehensive analysis revealed that an incremental irrigation frequency progressively enhanced plant morphological traits (height, upper three-leaf area), population dynamics (leaf area index, dry matter accumulation), and physiological performance (flag leaf SPAD, net photosynthetic rate), all peaking under the W2 and W3 treatments. While yield components and total water consumption exhibited linear increases with irrigation inputs, grain yield demonstrated a parabolic response, reaching maxima under W2 (29.3% increase over W0) and W3 (29.1%), whereas water use efficiency (WUE) displayed a distinct inverse trend, with W2 achieving the optimal balance (4.6% reduction vs. W0). The grain quality parameters exhibited divergent responses: the starch content increased proportionally with irrigation, while protein-associated indices (wet gluten, sedimentation value) and dough rheological properties (stability time, extensibility) peaked under W2. Notably, protein content and its subcomponents followed a unimodal pattern, with the W0, W1, and W2 treatments surpassing W3 by 3.4, 11.6, and 11.3%, respectively. Strong correlations emerged between protein composition and processing quality, while regression modeling identified an optimal water consumption threshold (3250~3500 m³ ha⁻¹) that concurrently maximized grain yield, protein output, and WUE. The W2 regime achieved the synchronization of water conservation, yield preservation, and quality enhancement through strategic irrigation timing during critical growth phases. These findings establish a scientifically validated framework for sustainable, intensive wheat production in arid irrigation districts, resolving the tripartite challenge of water scarcity mitigation, food security assurance, and processing quality optimization through precision water management. Full article

A study was conducted using 144 Hy-Line Brown laying hens (22 weeks old) to assess the impact of exogenous enzymes on energy utilisation and ileal nutrient digestibility in diets containing 300 g/kg wheat distillers’ dried grains with solubles (DDGS). A basal diet was prepared and divided into eight treatments: a control (C) and diets supplemented with 2000 units/kg xylanase (XYL), 500 units/kg phytase (PHY), and 4000 units/kg protease (PRO), individually and in combination. The diets were fed for nine days to six coops, each housing three birds. Feed intake, weight gain, and feed conversion ratio were recorded. The feed and excreta were analysed for gross energy, and the apparent metabolisable energy (AME) was calculated. On the final day, the birds were euthanised, and ileal digesta were collected, freeze-dried, and analysed for the digestibility coefficients of dry matter (DM), nitrogen, fat, and neutral detergent fibres (NDFs). XYL supplementation improved (p = 0.035) dietary AME but did not affect (p > 0.05) DM, nitrogen, fat, or NDF digestibility. No significant effects (p > 0.05) were observed for PHY or PRO, and no interactions (p > 0.05) were found between enzyme combinations. Substrates in experimental diets involving various enzyme combinations should be given careful consideration. Full article

The objective of this study was to compare the effects of applying a 50% wheat grain diet with those of a diet with 3% additional dietary fibre from various sources on the development of broiler chickens’ gastrointestinal tract and its related organs and to model this phenomenon based on data obtained from 35-day-old chickens using multiple regression equations. The use of various structural components, including oat hull (OH), sunflower hull (SH), sugar beet pulp (SBP), and wheat bran (WB), in proportions of 3% of the diet not only affects digestive processes in broiler chickens’ gastrointestinal tract but also causes a change in the length of their intestinal sections or the weight of related organs. These effects can be taken into account when creating an experimental model, the results of which can at least be partially applicable to human studies. The use of OH and SH (3%) in the birds’ diets resulted in a significantly higher body weight (p < 0.05) compared with the use of SBP and WB. OH in the diet significantly increased (p < 0.01) the weight of the chicken’s gizzards compared with the other dietary fibre sources, apart from SH. On the other hand, the weight of the proventriculus in chickens fed the diet containing OH was significantly lower than that of the chickens fed the diet containing SBP (p < 0.05). The use of SH in the diet caused a significant decrease (p < 0.01) in the weight of the chickens’ heart. Compared with other additional sources of dietary fibre, OH in the diet also significantly increased (p < 0.05) the lengths of the small and large intestines, as well as the total length of the intestines. A correlation analysis showed a significant, average, positive relationship (p < 0.05) between the content of TDF in the diet and the weight of the gizzard and indicated a significant positive correlation between the lengths of the jejunum and the remaining sections of the intestines. Additionally, the regression equation models indicated a significant effect (p < 0.01) of all the independent variables on the jejunal, ileal, and caecal lengths and the liver weight. The application of the regression model confirmed significant changes in the small intestine and liver weight depending on the type of dietary fibre and other independent variables, which can also be taken into account when assessing diseases in people with thin intestines. However, further studies with separate models still need to be conducted using experiments including both soluble and insoluble fibre. Full article