Search Results (516)

This study examines the influence of different printing orientations and infill settings on the strength and flexibility of components produced using resin-based 3D printing, particularly with masked stereolithography (MSLA). Using a common photopolymer resin and a widely available desktop MSLA printer, we produced and tested a series of samples with varying tilt angles and internal structures. To understand their mechanical behavior, we applied a custom bending test combined with high-precision deformation tracking through the GOM ARAMIS digital image correlation system. The results obtained clearly show that both the angle of printing and the density of the internal infill structure play a significant role in how much strain the printed parts can handle before breaking. Notably, a 75° orientation provided the best deformation performance, and infill rates between 60% and 90% offered a good balance between strength and material efficiency. These findings highlight how adjusting print settings can lead to stronger parts while also saving time and resources—an important consideration for practical applications in engineering, design, and manufacturing. Full article

An in-depth investigation into the evolutionary characteristics, transmission mechanisms, and optimization pathways of electricity consumption linkages across China’s industrial sectors highlights their substantial theoretical and practical significance in achieving the “dual carbon” goals and advancing high-quality economic development. This study investigates the structural characteristics and developmental trends of electricity consumption linkages across China’s industrial sectors using an enhanced hypothetical extraction method. The analysis draws on national input–output tables and sector-specific electricity consumption data during the period from 2002 to 2020. Key transmission routes between industrial sectors are identified through path analysis and average path length calculations. The findings reveal that China’s industrial electricity consumption structure is marked by notable scale expansion and differentiation. The magnitude of inter-sectoral electricity flows continues to grow steadily. The evolution of these linkages exhibits clear phase-specific patterns, while the intensity of electricity consumption connections across sectors shows pronounced heterogeneity. Furthermore, the transmission path analysis revealed differentiated characteristics of electricity influence transmission, with generally shorter internal paths within sectors, significant cross-sectoral transmission differences, and manufacturing demonstrating good transmission accessibility with moderate path distances to major sectors. These insights provide a robust foundation for designing differentiated energy conservation policies, as well as for optimizing the overall structure of industrial electricity consumption. Full article

Organoids allow to model healthy and diseased human tissues. and have applications in developmental biology, drug discovery, and cell therapy. Traditionally cultured in immersion/suspension, organoids face issues like lack of standardization, fusion, hypoxia-induced necrosis, continuous agitation, and high media volume requirements. To address these issues, we developed an air–liquid interface (ALi) technology for culturing organoids, termed AirLiwell. It uses non-adhesive microwells for generating and maintaining individualized organoids on an air–liquid interface. This method ensures high standardization, prevents organoid fusion, eliminates the need for agitation, simplifies media changes, reduces media volume, and is compatible with Good Manufacturing Practices. We compared the ALi method to standard immersion culture for midbrain organoids, detailing the process from human pluripotent stem cell (hPSC) culture to organoid maturation and analysis. Air–liquid interface organoids (3D-ALi) showed optimized size and shape standardization. RNA sequencing and immunostaining confirmed neural/dopaminergic specification. Single-cell RNA sequencing revealed that immersion organoids (3D-i) contained 16% fibroblast-like, 23% myeloid-like, and 61% neural cells (49% neurons), whereas 3D-ALi organoids comprised 99% neural cells (86% neurons). Functionally, 3D-ALi organoids showed a striking electrophysiological synchronization, unlike the heterogeneous activity of 3D-i organoids. This standardized organoid platform improves reproducibility and scalability, demonstrated here with midbrain organoids. The use of midbrain organoids is particularly relevant for neuroscience and neurodegenerative diseases, such as Parkinson’s disease, due to their high incidence, opening new perspectives in disease modeling and cell therapy. In addition to hPSC-derived organoids, the method’s versatility extends to cancer organoids and 3D cultures from primary human cells. Full article

Packaging waste is a major environmental issue, making the transition to sustainable solutions imperative. This article proposes the concept of Shared Producer Responsibility (SPR) as a key approach to advancing sustainable packaging in the fast-moving consumer goods (FMCG) sector. The study explores how the United Nations Sustainable Development Goals (SDGs), environmental, social, and governance (ESG) reporting, and stakeholder engagement converge to support this transition. The research identifies current trends, challenges, and gaps in sustainable packaging practices through a systematic literature review (SLR) and analysis of sustainability and ESG reports from leading FMCG and packaging companies. The findings highlight the need for standardised reporting frameworks and improved stakeholder cooperation to enhance transparency and accountability in sustainability efforts. This study proposes a conceptual framework for accelerating sustainable packaging adoption through combining strategies like consumer education, regulatory incentives, and clear product labelling. The proposal to implement the concept of Shared Producer Responsibility emphasises the shared accountability of FMCG companies and packaging manufacturers in managing the full environmental lifecycle of packaging materials. This approach is crucial for achieving SDG 12 (responsible consumption and production) and SDG 13 (climate action) and driving more effective and sustainable packaging practices across the FMCG industry. Full article

Hot work die steel is an alloy steel with good high-temperature performance, which is widely used in mechanical manufacturing, aerospace, and other fields. During the working process of hot working mold steel, it is subjected to high temperature, wear, and other effects, which can lead to a decrease in the surface hardness of the mold, accelerate surface damage, shorten the service life, and reduce the quality of the workpiece. In order to improve the wear resistance of the mold, this paper conducts two surface treatments, chrome plating and nitriding, on the surface of hot work mold steel, and compares the high-temperature wear behavior of the materials after the two surface treatments. The results indicate that the hot work die steel obtained higher surface hardness and wear resistance after nitriding surface modification. After nitriding treatment, the surface of hot work die steel contains ε phase (Fe_2–3N), which improves its surface hardness and wear resistance, thus exhibiting better surface hardness and wear resistance than the chrome-plated sample. In this study, the high-temperature wear behavior of hot work die steel after two kinds of surface strengthening treatments was deeply discussed, and the high-temperature wear mechanism of steel after surface strengthening was revealed. It provides a theoretical basis and experimental basis for the surface modification of hot working die steel, and also provides new ideas and methods for improving the service life and workpiece quality of hot working die steel in industrial production. In this study, the advantages and disadvantages of high-temperature wear resistance of hot working die steel after chromium plating and nitriding were systematically compared for the first time, which provided a scientific basis for the selection of surface strengthening technology of hot working die steel and had important academic value and practical application significance. Full article

The cryopreservation of three-dimensional (3D) biofabricated constructs is a key enabler for their clinical application in regenerative medicine. Unlike two-dimensional (2D) cultures, 3D systems such as encapsulated cell spheroids, molded hydrogels, and bioprinted tissues present specific challenges related to cryoprotectant (CPA) diffusion, thermal gradients, and ice formation during freezing and thawing. This review examines the current strategies for preserving 3D constructs, focusing on the role of biomaterials as cryoprotective matrices. Natural polymers (e.g., hyaluronic acid, alginate, chitosan), protein-based scaffolds (e.g., silk fibroin, sericin), and synthetic polymers (e.g., polyethylene glycol (PEG), polyvinyl alcohol (PVA)) are evaluated for their ability to support cell viability, structural integrity, and CPA transport. Special attention is given to cryoprotectant systems that are free of dimethyl sulfoxide (DMSO), and to the influence of hydrogel architecture on freezing outcomes. We have compared the efficacy and limitations of slow freezing and vitrification protocols and review innovative approaches such as temperature-controlled cryoprinting, nano-warming, and hybrid scaffolds with improved cryocompatibility. Additionally, we address the regulatory and manufacturing challenges associated with developing Good Manufacturing Practice (GMP)-compliant cryopreservation workflows. Overall, this review provides an integrated perspective on material-based strategies for 3D cryopreservation and identifies future directions to enable the long-term storage and clinical translation of engineered tissues. Full article

This study investigates a stochastic production planning problem with regime-switching parameters, inspired by economic cycles impacting production and inventory costs. The model considers types of goods and employs a Markov chain to capture probabilistic regime transitions, coupled with a multidimensional Brownian motion representing stochastic demand dynamics. The production and inventory cost optimization problem is formulated as a quadratic cost functional, with the solution characterized by a regime-dependent system of elliptic partial differential equations (PDEs). Numerical solutions to the PDE system are computed using a monotone iteration algorithm, enabling quantitative analysis. Sensitivity analysis and model risk evaluation illustrate the effects of regime-dependent volatility, holding costs, and discount factors, revealing the conservative bias of regime-switching models when compared to static alternatives. Practical implications include optimizing production strategies under fluctuating economic conditions and exploring future extensions such as correlated Brownian dynamics, non-quadratic cost functions, and geometric inventory frameworks. In contrast to earlier studies that imposed static or overly simplified regime-switching assumptions, our work presents a fully integrated framework—combining optimal control theory, a regime-dependent system of elliptic PDEs, and comprehensive numerical and sensitivity analyses—to more accurately capture the complex stochastic dynamics of production planning and thereby deliver enhanced, actionable insights for modern manufacturing environments. Full article

Starting Active Materials for Synthesis (SAMS) represents a critical stage in drug manufacturing, directly influencing the microbiological quality and safety of the final product. The introduction of SAMS marks the point where Good Manufacturing Practices (GMP) begin to apply, which are essential for ensuring sterility and preventing microbial contamination during the synthesis process. However, defining the exact point in the process that qualifies as the SAMS is subject to uncertainties, as earlier stages are not always governed by stringent GMP standards. The regulatory differences between various countries further contribute to this issue. This study explores the implications of SAMS selection and use in relation to sterility and infection control, analyzing the guidelines of major Regulatory Authorities and comparing their approaches to GMP. Regulations from several international regulatory agencies were examined, with a particular focus on microbiological control measures and infection protection in the SAMS manufacturing process. The analysis focused on the microbiological control requirements and safety measures applicable to the stages preceding the introduction of SAMS into the production of the final Active Pharmaceutical Ingredients (APIs). Documents published between 2015 and 2025 were included based on predefined criteria regarding relevance, accessibility, and regulatory authority. The analysis revealed significant discrepancies between regulations regarding the definition and management of SAMS. In particular, the regulations in Mexico and India have notable gaps, failing to provide clear guidelines on SAMS sterility and protection against infectious contamination. Conversely, China has introduced risk-based approaches and early-stage microbiological controls, especially for sterile products, aligning with international standards. The European Medicines Agency (EMA), the U.S. Food and Drug Administration (FDA), the Pharmaceutical Inspection Co-operation Scheme (PIC/S), and the World Health Organization (WHO) have well-established systems for microbiological quality control of SAMS, including rigorous measures for the validation of suppliers and risk management to ensure that SAMS does not compromise the microbiological safety of the final product. The regulations in Brazil and Canada introduce additional measures to protect the microbiological quality of SAMS, with specifications for contamination control and certification of critical stages. The lack of a harmonized language for the definition of SAMS, coupled with a fragmented regulatory framework, presents a challenge for infection protection in pharmaceutical manufacturing. Key issues include the absence of specific regulations for stages prior to the introduction of SAMS and the lack of standards for inspections related to these stages. A desirable solution would be the mandatory extension of GMPs to the stages before SAMS introduction, with centralized control to ensure sterility and protection against infection throughout the entire manufacturing process. Full article

Sustainability and the circular economy are increasingly recognized as global priorities, particularly in industrial waste management. This study explores the development of a sustainable composite material using wood waste and natural fibers, contributing to circular economy practices. Sandwich panels were manufactured with a green epoxy resin matrix, incorporating wood waste in the core and flax fibers in the outer layers. Mechanical tests on the sandwich panel revealed a facing bending stress of 92.79 MPa and a core shear stress of 2.43 MPa. The panel demonstrated good compressive performance, with an edgewise compressive strength of 61.39 MPa and a flatwise compressive strength of 96.66 MPa. The material’s viscoelastic behavior was also characterized. In stress relaxation tests (from an initial 21 MPa), the panel’s stress decreased by 20.2% after three hours. The experimental relaxation data were successfully fitted by the Kohlrausch–Williams–Watts (KWW) model for both short- and long-term predictions. In creep tests, the panel showed a 21.30% increase in displacement after three hours under a 21 MPa load. For creep behavior, the KWW model was preferable for short-term predictions, while the Findley model provided a better fit for long-term predictions. Full article

With the increasing demand for food quality and the need for green and sustainable development of food packaging materials in the environment, the preparation and optimization of multifunctional natural and renewable antibacterial packaging materials have become an important trend. This article aims to explore the development of chitosan–cellulose composite materials with good antibacterial properties and promote the widespread application of chitosan and cellulose in food packaging materials. Combining various natural polysaccharide polymers, we discuss the application of chitosan cellulose in meat, dairy products, fruits and vegetables, and fishery products. Meanwhile, we explore their antibacterial and antioxidant behaviors during their use as food packaging materials. This provides a reference for effectively improving the performance of modified chitosan and cellulose food packaging materials in the future. Based on the above explanation, we analyzed the advantages and disadvantages of modified chitosan and cellulose and looked forward to the future development trends of chitosan and cellulose blend films in food preservation. Chitosan–cellulose blends not only have important prospects in food packaging and preservation applications, but can also be combined with intelligent manufacturing to enhance their food preservation performance. The aim of this review is to provide valuable references for basic research on the antimicrobial properties of these composites and their practical application in smart food packaging. Full article

Steak tartare is a ready-to-eat (RTE) meat product, prepared with finely chopped or ground raw beef, with a rich culinary history and increasing consumption trend in the last years. Yet, its microbiological safety and technological challenges remain largely under-investigated. This review analyses the regulations, the safety, and technological advances in steak tartare manufacturing, focusing on microbiological risks due to potential contamination by pathogens like Salmonella spp., Listeria monocytogenes, and Escherichia coli O157:H7. From this perspective, the outbreaks associated with the consumption of raw meat products have confirmed the importance of good hygiene practice and process control, currently based on the presence of nitrite in the formulation and accurate cold chain management. Recently, the EU regulations have set stricter limits for the use of nitrites and nitrates in meat products, and this evolution has increased the interest in natural alternatives. The scientific literature indicates that plant-based antimicrobials, high-pressure processing (HPP), and novel starter cultures can be promising tools to improve raw meat safety and shelf life. This review analyses the possible options for nitrite replacement, which might involve combined interventions with natural antimicrobials, starter cultures, and packaging solutions. Future studies need to address the microbial behaviour and dynamics in nitrite-free formulations, including safety validation by challenge testing with foodborne pathogens. In this respect, steak tartare could be a model for innovation in the meat industry. However, considering the challenges that must be faced, collaboration across disciplines will be essential to meet regulatory constraints and consumer expectations while ensuring product quality and safety. Full article

The objective of this study was to identify surface geometric deviations that may adversely affect the operational properties of bearings, including vibration, noise, and service life. A comprehensive prediction model is presented that combines a fundamental trend expressed by a power function with periodic oscillations, whose influence gradually diminishes with exponential decay. The model was calibrated using the experimental data obtained from 17 industrial RA-608-338 rolling bearing rings manufactured from high-carbon, low-alloy 100Cr6 steel. An excellent goodness-of-fit (R² exceeding 0.98) and minimal root-mean-square error (RMSE) were achieved. The proposed procedure provides a clear physical interpretation of the model’s subcomponents, while facilitating straightforward implementation in real production processes for continuous quality control and predictive maintenance purposes. This paper also includes a detailed description of the methodology, data processing, experimental results, comparison of multiple model variants, interactive visualization of the results on a logarithmic scale, and recommendations for practical application. Full article

Accurate profiling of thread-milling cutters is one of the main prerequisites of high-precision internal thread production. Despite the accuracy of analytical methods, their complexity in most instances makes practical application an issue. The current research addresses a graphical profiling method, the Tangent Circles Method, introduced by the authors for the facilitation of thread-milling tool geometric design. Several thread-milling cutters were designed employing this method, and their working surface profiles were compared with those obtained analytically. The comparison showed a good match, with profile deviations within acceptable manufacturing limits. CAD-based simulations also demonstrated that rake angle and relief height parameters significantly influence the resulting tool geometry. The results validate the Tangent Circles Method as a mathematically sound and industrially viable tool-profiling method for industry use. Its easy application and precision make it a reliable choice over analytical methods in internal thread-milling cutters’ industrial design. Full article

Medicinal products available on the market should be characterised by therapeutic efficacy, high quality, and safety for patients. They must either be sterile or comply with the appropriate pharmacopoeial microbiological purity requirements. Pharmacopoeial monographs related to microbiological tests of drug quality were also referenced. Despite stringent regulations governing pharmaceutical production, irregularities in the microbiological quality of drugs still occur. These are monitored by relevant agencies, which may order the recall of defective product batches from the market. However, in recent years, numerous cases of microbiological contamination in drugs and drug-related infections have been reported. Both isolated incidents and larger outbreaks or epidemics linked to contaminated medicines have been documented. Various microorganisms, including Gram-negative and Gram-positive bacteria, anaerobes, and yeast-like and mould fungi, have been identified in medicinal products or in patients affected by contaminated drugs. Ensuring the appropriate purity or sterility of pharmaceutical raw materials; maintaining cleanliness in the manufacturing environment, facilities, and equipment; and adhering to hygiene protocols and Good Manufacturing Practice regulations are essential for the production of safe and high-quality medicinal products. The aim of this study is to collect and compile information on the microbiological quality of drugs available on the market, with particular attention to identified irregularities, objectionable microorganisms isolated from medicinal products, and drug-related infections. Full article

Probabilistic selling is a retail strategy in which consumers purchase products without knowing their exact identities until after purchase, with various applications like gaming and retail; a real-world practice involves retailers may unsealing and reselling goods to meet consumer demand for transparency. This disrupts manufacturers’ strategies designed to adopt the uncertainty for segmentation and pricing. Using a vertically differentiated supply chain model structured as a Stackelberg game framework, this study examines how transparency from retailer unsealing affects profitability, consumer surplus, and market dynamics. Key findings include the following: (1) Unsealing increases retailer profits by aligning pricing with heterogeneous consumer willingness to pay. (2) Introducing a manufacturer’s direct channel reduces unsealing profits via price competition. (3) Unsealing creates conflicts between manufacturers’ design goals and retailers’ profit-driven incentives. By applying a Stackelberg game framework to model unsealing as a downstream transparency decision, this work advances the probabilistic selling literature by offering a structured approach to analyzing how downstream transparency and retailer strategies reshape probabilistic selling and supply chain dynamics. It highlights the need for manufacturers to balance segmentation, pricing, and channel control, offering insights into mitigating conflicts between design intentions and downstream market behaviors. Full article