You are currently on the new version of our website. Access the old version .

2,016 Results Found

  • Communication
  • Open Access
997 Views
7 Pages

A Prospective Cohort Study Comparing Microscopy and Culture in the Diagnosis of Superficial Fungal Skin Infections

  • Amelia Yuting Monteiro,
  • Hui Mei Cheng,
  • Larissa Lim,
  • Jiun Yit Pan,
  • Kun Liang and
  • Hong Liang Tey

29 October 2025

Superficial fungal skin infections are common but often misdiagnosed, which may result in inappropriate treatment and the worsening of symptoms. An accurate and timely diagnosis is essential to differentiate these infections from similar conditions s...

  • Article
  • Open Access
10 Citations
4,580 Views
11 Pages

Purpose: To investigate the characteristics of in vitro culture and in vivo confocal microscopy (IVCM) in patients with fungal keratitis (FK) presented in a tertiary referral hospital in central China. Methods: In this noncomparative retrospective st...

  • Article
  • Open Access
3 Citations
2,046 Views
18 Pages

We examined fungal communities in soil profiles of 0–10 cm depth along the altitudinal gradient of 250–530–990 m.a.s.l. at the Central Negev Desert, Israel, which benefit from similar annual precipitation (95 mm). In the soil sample...

  • Article
  • Open Access
10 Citations
4,513 Views
12 Pages

Diagnosis of Fungal Keratitis in Low-Income Countries: Evaluation of Smear Microscopy, Culture, and In Vivo Confocal Microscopy in Nepal

  • Jeremy J. Hoffman,
  • Reena Yadav,
  • Sandip Das Sanyam,
  • Pankaj Chaudhary,
  • Abhishek Roshan,
  • Sanjay Kumar Singh,
  • Simon Arunga,
  • Victor H. Hu,
  • David Macleod and
  • Matthew J. Burton

13 September 2022

Clinically diagnosing fungal keratitis (FK) is challenging; diagnosis can be assisted by investigations including in vivo confocal microscopy (IVCM), smear microscopy, and culture. The aim of this study was to estimate the sensitivity in detecting fu...

  • Feature Paper
  • Article
  • Open Access
2,586 Views
12 Pages

Comparison of Fungal and Non-Fungal Rhinosinusitis by Culture-Based Analysis

  • Chih-Hung Cha,
  • Wei-Chih Chen,
  • Yu-Ming Wang,
  • Shao-Chun Wu,
  • Tai-Jan Chiu,
  • Ching-Nung Wu,
  • Yinshen Wee,
  • Ching-Shuen Wang,
  • Yao-Hsu Yang and
  • Sheng-Dean Luo

11 September 2023

Background: Incidence of fungal rhinosinusitis has increased in recent few years. We investigated the differences in microbiological findings between patients with fungal and non-fungal rhinosinusitis by growing microbiological cultures from samples...

  • Article
  • Open Access
6 Citations
2,352 Views
17 Pages

31 October 2022

Pinosylvin stilbenes are natural phenolic compounds found in the Pinaceae family and act as phytoalexins. Some pinosylvin stilbenes have strong nematicidal activity against pine wood nematodes (PWNs: Bursaphelenchus xylophilus). Here, we established...

  • Article
  • Open Access
12 Citations
4,255 Views
14 Pages

23 November 2019

Studies of fungi in caves have become increasingly important with the advent of white-nose syndrome (WNS), a disease caused by the invasive fungus Pseudogymnoascus destructans (Pd) that has killed an estimated 6.5 million North American bats. We swab...

  • Feature Paper
  • Article
  • Open Access
1 Citations
2,152 Views
13 Pages

9 June 2023

This study aimed to select the most effective culture extracts for controlling honeybee nosemosis using 342 entomopathogenic fungi of 24 species from 18 genera. The germination inhibitory activity of the fungal culture extract on Nosema ceranae spore...

  • Review
  • Open Access
29 Citations
8,406 Views
16 Pages

The Potential Use of Fungal Co-Culture Strategy for Discovery of New Secondary Metabolites

  • Shuang Xu,
  • Mengshi Li,
  • Zhe Hu,
  • Yilan Shao,
  • Jialiang Ying and
  • Huawei Zhang

Fungi are an important and prolific source of secondary metabolites (SMs) with diverse chemical structures and a wide array of biological properties. In the past two decades, however, the number of new fungal SMs by traditional monoculture method had...

  • Article
  • Open Access
17 Citations
4,566 Views
16 Pages

11 August 2021

Fungi associated with macroalgae are less known when compared with those on wood in the marine environment. In this study, we assessed the diversity of fungi associated with the red alga Pterocladiella capillacea at Chao-Jin Park, Keelung, Taiwan. Al...

  • Article
  • Open Access
1 Citations
3,599 Views
18 Pages

27 January 2024

Co-cultivation, coupled with the OSMAC approach, is considered an efficient method for expanding microbial chemical diversity through the activation of cryptic biosynthetic gene clusters (BGCs). As part of our project aiming to discover new fungal me...

  • Article
  • Open Access
11 Citations
2,529 Views
11 Pages

21 October 2021

Isolated boxwood endophytes have been demonstrated to effectively protect boxwood plants from infection by Calonectria pseudonaviculata (Cps). However, the roles of endophytes as communities in plant defense are not clear. Here, we demonstrated diffe...

  • Article
  • Open Access
15 Citations
4,199 Views
11 Pages

Evaluation of a New Culture Protocol for Enhancing Fungal Detection Rates in Respiratory Samples of Cystic Fibrosis Patients

  • Tobias G.P. Engel,
  • Marlou Tehupeiory-Kooreman,
  • Willem J.G. Melchers,
  • Monique H. Reijers,
  • Peter Merkus and
  • Paul E. Verweij

9 June 2020

Cystic fibrosis (CF) can be complicated by fungal infection of the respiratory tract. Fungal detection rates in CF sputa are highly dependent on the culture protocol and incubation conditions and thus may lead to an underestimation of the true preval...

  • Article
  • Open Access
4 Citations
7,926 Views
10 Pages

28 February 2023

Rapid diagnosis of dermatophytosis is essential for early treatment induction and prevention of spreading to other animals and humans. No single diagnostic test is identified as the “gold standard”. The purpose of the study was to evaluat...

  • Article
  • Open Access
77 Citations
12,221 Views
13 Pages

10 July 2014

Microalgae harvesting is a labor- and energy-intensive process and new approaches to harvesting microalgae need to be developed in order to decrease the costs. In this study; co-cultivatation of filamentous fungus (Aspergillus niger) and microalgae...

  • Article
  • Open Access
681 Views
16 Pages

Culture Collections for Conservation Ex Situ: Characterization and Biotechnological Application Potential of Saprotrophic Fungal Strains from Brazil

  • Simone Buratti,
  • Carolina Elena Girometta,
  • Lorenzo Goppa,
  • Claudia Picozzi,
  • Paola Rossi,
  • Elena Savino,
  • Maria Letizia Gargano,
  • Giuseppe Venturella,
  • Adriana de Mello Gugliotta and
  • Manuela Rollini

Saprotrophic and lignocellulolytic fungi from tropical areas especially represent a promising yet relatively underexplored frontier for both taxonomy and applied research. This makes ex situ conservation through culture collections of paramount impor...

  • Article
  • Open Access
5 Citations
3,958 Views
12 Pages

With blood stream infections (BSIs) representing a major cause of mortality and morbidity worldwide, blood cultures play a crucial role in diagnosis, but their clinical application is dampened by the long turn-around time and the detection of only cu...

  • Article
  • Open Access
1 Citations
1,542 Views
24 Pages

Cultivation of Fungal Endophytes with Tissue Culture Grapevine Seedlings Reprograms Metabolism by Triggering Defence Responses

  • Xiaoxia Pan,
  • Huizhi Liu,
  • Yiqian Li,
  • Lirong Guo,
  • Yunuo Zhang,
  • Youyong Zhu and
  • Mingzhi Yang

In this study, the transcriptome profiles of tissue–cultured grapevine (Vitis vinifera L. × Vitis labrusca L.: Rose Honey) seedlings inoculated with fungal endophytes Epicoccum layuense R2-21 (Epi R2-21) and Alternaria alternata XHYN2 (Al...

  • Article
  • Open Access
6 Citations
2,240 Views
12 Pages

Sod Culture with Vicia villosa Alters the Diversity of Fungal Communities in Walnut Orchards for Sustainability Development

  • Wan-Xia He,
  • Qiao-Feng Sun,
  • Abeer Hashem,
  • Elsayed Fathi Abd_Allah,
  • Qiang-Sheng Wu and
  • Yong-Jie Xu

7 July 2023

Monoculture frequently causes loss of soil nutrients and the emergence of soil-borne diseases in walnut orchards, whereas it is unknown whether sod culture with Vicia villosa (a popular agroforestry system) in walnut orchards impacts the structural c...

  • Article
  • Open Access
36 Citations
3,215 Views
11 Pages

Targeted High-Throughput Sequencing Identifies Predominantly Fungal Pathogens in Patients with Clinically Infectious, Culture-Negative Endophthalmitis in South India

  • Jaishree Gandhi,
  • Rajagopalaboopathi Jayasudha,
  • Poonam Naik,
  • Savitri Sharma,
  • Vivek Pravin Dave and
  • Joveeta Joseph

To evaluate the clinical utility of high-throughput sequencing (HTS) approach-based analysis of the bacterial and fungal genome in vitreous fluids from patients clinically diagnosed as endophthalmitis, we subjected 75 vitreous fluids from clinically...

  • Article
  • Open Access
2,665 Views
17 Pages

Optimization of Aspergillus versicolor Culture and Aerosolization in a Murine Model of Inhalational Fungal Exposure

  • Catherine B. Blackwood,
  • Tara L. Croston,
  • Mark A. Barnes,
  • Angela R. Lemons,
  • Rachael E. Rush,
  • Travis Goldsmith,
  • Walter G. McKinney,
  • Stacey Anderson,
  • Kelly L. Weaver and
  • Brett Green
  • + 3 authors

8 November 2023

Aspergillus versicolor is ubiquitous in the environment and is particularly abundant in damp indoor spaces. Exposure to Aspergillus species, as well as other environmental fungi, has been linked to respiratory health outcomes, including asthma, aller...

  • Article
  • Open Access
5 Citations
4,519 Views
14 Pages

Fungal F8-Culture Filtrate Induces Tomato Resistance against Tomato Yellow Leaf Curl Thailand Virus

  • Yi-Shu Chiu,
  • Yuh Tzean,
  • Yi-Hui Chen,
  • Chi-Wei Tsai and
  • Hsin-Hung Yeh

23 July 2021

Tomato (Solanum lycopersicum) is an important economic crop worldwide. However, tomato production is jeopardized by the devastating tomato yellow leaf curl disease caused by whitefly-transmitted begomoviruses (WTBs). In this study, we evaluated the e...

  • Article
  • Open Access
30 Citations
4,359 Views
25 Pages

21 October 2022

Fungal organisms are considered one of the most relevant stone colonizers, and biodeteriogens. They are ubiquitous heterotrophs, metabolically versatile, ranging from generalist to extremophiles. Limestone, a sedimentary rock characterized by high le...

  • Article
  • Open Access
1,650 Views
18 Pages

Biocide-Containing Facades Alter Culture-Based Bacterial and Fungal Community Composition and Resistance Patterns to Octylisothiazolinone

  • Michał Ciok,
  • Julia Diener,
  • Franziska Otte,
  • Julie Feimer,
  • Moritz Nichterlein,
  • Stefan Kalkhof and
  • Matthias Noll

Microbial communities are known to colonize biocide-free (BFFs) and even biocide-containing façades (BCFs) under various environmental conditions, leading to loss of value of façades due to biologically caused aging and discoloration. T...

  • Article
  • Open Access
664 Views
11 Pages

16 November 2025

Background: Coccidioidomycosis is an endemic fungal infection in the southwestern United States that can present as solitary lung nodules, mimicking malignancy on imaging. Molecular testing, such as PCR, offers rapid diagnosis but its performance in...

  • Article
  • Open Access
1,470 Views
19 Pages

Airborne Fungal Spore Diversity Assessment Using Culture-Dependent and Metabarcoding Approaches in Bat-Inhabited Natural and Anthropogenic Roosts in Portugal

  • Jaqueline T. Bento,
  • Guilherme Moreira,
  • Eugénia Pinto,
  • Priscilla Gomes da Silva,
  • Hugo Rebelo,
  • Joana Mourão,
  • Sofia I. V. Sousa and
  • João R. Mesquita

6 May 2025

Cave environments represent extreme and underexplored ecosystems wherein fungi play a crucial role in nutrient cycling and ecological dynamics. This study provides the first comprehensive assessment of fungal diversity in air samples from caves acros...

  • Article
  • Open Access
4 Citations
3,896 Views
19 Pages

Metabolomic Analysis of The Chemical Diversity of South Africa Leaf Litter Fungal Species Using an Epigenetic Culture-Based Approach

  • Rachel Serrano,
  • Víctor González-Menéndez,
  • Germán Martínez,
  • Clara Toro,
  • Jesús Martín,
  • Olga Genilloud and
  •  José R. Tormo

14 July 2021

Microbial natural products are an invaluable resource for the biotechnological industry. Genome mining studies have highlighted the huge biosynthetic potential of fungi, which is underexploited by standard fermentation conditions. Epigenetic effector...

  • Article
  • Open Access
8 Citations
3,109 Views
22 Pages

Diversity of Mycorrhizal Fungi in Temperate Orchid Species: Comparison of Culture-Dependent and Culture-Independent Methods

  • Sophie Mennicken,
  • Caio César Pires de Paula,
  • Hélène Vogt-Schilb and
  • Jana Jersáková

23 January 2024

Many orchid species are endangered due to anthropogenic pressures such as habitat destruction and overharvesting, meanwhile, all orchids rely on orchid mycorrhizal fungi (OMF) for seed germination and seedling growth. Therefore, a better understandin...

  • Article
  • Open Access
8 Citations
3,113 Views
14 Pages

14 April 2022

Fungal–bacterial co-culturing is a potential technique for the production of secondary metabolites with antibacterial activity. Twenty-nine fungal species were screened in a co-culture with carbapenem-resistant Klebsiella pneumoniae at differen...

  • Review
  • Open Access
63 Citations
9,899 Views
25 Pages

Recent Perspectives in the Management of Fungal Keratitis

  • Nimmy Raj,
  • Murugesan Vanathi,
  • Nishat Hussain Ahmed,
  • Noopur Gupta,
  • Neiwete Lomi and
  • Radhika Tandon

26 October 2021

Mycotic keratitis is common in warm, humid regions with a varying profile of pathogenic fungi according to geographical origin, socioeconomic status, and climatic condition. Clinical diagnosis can be challenging in difficult cases and those refractor...

  • Article
  • Open Access
16 Citations
6,071 Views
13 Pages

Clinical Diagnosis and Laboratory Testing of Abnormal Appearing Toenails: A Retrospective Assessment of Confirmatory Testing for Onychomycosis in the United States, 2022–2023

  • Aditya K. Gupta,
  • Tong Wang,
  • Elizabeth A. Cooper,
  • Sara A. Lincoln,
  • Hui-Chen Foreman,
  • William P. Scherer and
  • Wayne L. Bakotic

13 February 2024

Onychomycosis is an under-recognized healthcare burden. Despite the risk of misdiagnosis, confirmatory laboratory testing is under-utilized. Histopathologic examination with polymerase chain reaction (PCR) is currently the most effective diagnostic m...

  • Review
  • Open Access
33 Citations
6,358 Views
31 Pages

15 March 2022

Wall paintings have been a cultural expression of human creativity throughout history. Their degradation or destruction represents a loss to the world’s cultural heritage, and fungi have been identified as a major contributor to their decay. We...

  • Article
  • Open Access
9 Citations
3,119 Views
13 Pages

15 December 2022

Mycorrhizal specificity, i.e., the range of fungi allowing mycorrhizal partnerships, differs among orchid species, but that at early developmental stages is unclear. We investigated whether mycorrhizal specificity during seed germination and seedling...

  • Article
  • Open Access
8 Citations
2,848 Views
26 Pages

10 October 2023

Over the last decade, fungal conservation has become an increasingly important topic, especially for species tied to forest ecosystems. Among these, wood decay fungi are a group of interesting species from ecological and applicative points of view. C...

  • Article
  • Open Access
21 Citations
5,038 Views
11 Pages

Identification of Dermatophyte and Non-Dermatophyte Agents in Onychomycosis by PCR and DNA Sequencing—A Retrospective Comparison of Diagnostic Tools

  • Isabella Pospischil,
  • Charlotte Reinhardt,
  • Olympia Bontems,
  • Karine Salamin,
  • Marina Fratti,
  • Gabriela Blanchard,
  • Yun-Tsan Chang,
  • Helga Wagner,
  • Philipp Hermann and
  • Emmanuella Guenova
  • + 1 author

27 September 2022

Rapid and reliable fungal identification is crucial to delineate infectious diseases, and to establish appropriate treatment for onychomycosis. Compared to conventional diagnostic methods, molecular techniques are faster and feature higher accuracy i...

  • Article
  • Open Access
859 Views
10 Pages

Conventional Diagnostic Approaches to Dermatophytosis: Insights from a Three-Year Survey at a Public Dermatology Institute in Italy (2019–2021)

  • Eugenia Giuliani,
  • Maria Gabriella Donà,
  • Amalia Giglio,
  • Elva Abril,
  • Francesca Sperati,
  • Fulvia Pimpinelli and
  • Alessandra Latini

4 September 2025

Background/Objectives: Dermatophytosis is a widespread superficial fungal infection affecting skin, hair, and nails. Its diagnosis is often based on conventional methods such as microscopy and fungal culture. Laboratory confirmation is essential for...

  • Article
  • Open Access
6 Citations
6,479 Views
10 Pages

Blood culture systems are a potential alternative to classical cultivation of fungi on mycological media, but there are limited data on the suitability of these systems for culturing other sample types (e.g., sterile body fluids). We conducted a pros...

  • Review
  • Open Access
4 Citations
6,331 Views
18 Pages

A perforated peptic ulcer (PPU) is a surgical emergency with a high mortality rate. PPUs cause secondary peritonitis due to bacterial and fungal peritoneal contamination. Surgery is the main treatment modality and patient’s comorbidites impacts...

  • Article
  • Open Access
12 Citations
3,887 Views
13 Pages

22 August 2019

Artefact conditions need to be continuously monitored to avoid degradation effects naturally caused by time and public exploitation in order to increase the value of cultural assets. In this way, the atmospheric analysis of both biological and chemic...

  • Communication
  • Open Access
2 Citations
1,908 Views
8 Pages

Fungal Communities in Re-Emerging Fraxinus excelsior Sites in Lithuania and Their Antagonistic Potential against Hymenoscyphus fraxineus

  • Remigijus Bakys,
  • Gintarė Bajerkevičienė,
  • Alfas Pliūra,
  • Adas Marčiulynas,
  • Diana Marčiulynienė,
  • Jūratė Lynikienė,
  • Valeriia Mishcherikova and
  • Audrius Menkis

Fifty-nine fungal taxa, isolated from re-emerging Fraxinus excelsior sites in Lithuania, were in vitro tested against three strains of Hymenoscyphus fraxineus on agar media to establish their biocontrol properties. All tested fungi were isolated from...

  • Feature Paper
  • Article
  • Open Access
8 Citations
2,822 Views
13 Pages

Fungal Metabolites with Antagonistic Activity against Fungi of Lithic Substrata

  • Marco Masi,
  • Mariagioia Petraretti,
  • Antonino De Natale,
  • Antonino Pollio and
  • Antonio Evidente

16 February 2021

Fungi are among the biotic agents that can cause deterioration of building stones and cultural heritage. The most common methods used to control fungal spread and growth are based on chemical pesticides. However, the massive use of these synthetic ch...

  • Article
  • Open Access
31 Citations
7,573 Views
18 Pages

Dual RNA-Seq Analysis of Trichophyton rubrum and HaCat Keratinocyte Co-Culture Highlights Important Genes for Fungal-Host Interaction

  • Monise Fazolin Petrucelli,
  • Kamila Peronni,
  • Pablo Rodrigo Sanches,
  • Tatiana Takahasi Komoto,
  • Josie Budag Matsuda,
  • Wilson Araújo da Silva,
  • Rene Oliveira Beleboni,
  • Nilce Maria Martinez-Rossi,
  • Mozart Marins and
  • Ana Lúcia Fachin

19 July 2018

The dermatophyte Trichophyton rubrum is the major fungal pathogen of skin, hair, and nails that uses keratinized substrates as the primary nutrients during infection. Few strategies are available that permit a better understanding of the molecular me...

  • Article
  • Open Access
9 Citations
3,616 Views
25 Pages

10 September 2023

With an impressive ability to survive in harsh environments, black fungi are an ecological group of melanized fungi that are widely recognized as a major contributor to the biodeterioration of stone cultural heritage materials. As part of the ongoing...

  • Feature Paper
  • Article
  • Open Access
25 Citations
4,165 Views
16 Pages

Arthrobotrys musiformis (Orbiliales) Kills Haemonchus contortus Infective Larvae (Trichostronylidae) through Its Predatory Activity and Its Fungal Culture Filtrates

  • Gustavo Pérez-Anzúrez,
  • Agustín Olmedo-Juárez,
  • Elke von-Son de Fernex,
  • Miguel Ángel Alonso-Díaz,
  • Edgar Jesús Delgado-Núñez,
  • María Eugenia López-Arellano,
  • Manasés González-Cortázar,
  • Alejandro Zamilpa,
  • Ana Yuridia Ocampo-Gutierrez and
  • Pedro Mendoza-de Gives

20 September 2022

Haemonchus contortus (Hc) is a parasite affecting small ruminants worldwide. Arthrobotrys musiformis (Am) is a nematode-trapping fungi that captures, destroys and feeds on nematodes. This study assessed the predatory activity (PA) and nematocidal act...

  • Article
  • Open Access
3 Citations
4,627 Views
31 Pages

14 December 2023

Culture collections (CCs) play an important role in the ex situ conservation of biological material and maintaining species and strains, which can be used for scientific and practical purposes. The Komarov Botanical Institute Basidiomycetes Culture C...

  • Technical Note
  • Open Access
5 Citations
2,665 Views
6 Pages

Concentrations of atmospheric culturable bioaerosols at mountain and seashore sites were measured in field investigations by using a bio-culture sampler. The eastern Korean peninsula was selected for the measurements because of the short distance bet...

  • Article
  • Open Access
16 Citations
4,093 Views
24 Pages

Plant–Fungal Interactions: A Case Study of Epicoccoum nigrum Link

  • Rafał Ogórek,
  • Katarzyna Przywara,
  • Agata Piecuch,
  • Magdalena Cal,
  • Agnieszka Lejman and
  • Krzysztof Matkowski

1 December 2020

Epicoccum nigrum Link is a cosmopolitan species, and it has been described as both an in vitro and in vivo antagonist of many fungal pathogens of plants. However, there are no clear reports about the interactions between E. nigrum and various plant s...

  • Article
  • Open Access
9 Citations
5,132 Views
20 Pages

The main purpose of this study was to degrade total petroleum hydrocarbons (TPHs) from contaminated soil in batch microcosm reactors. Native soil fungi isolated from the same petroleum-polluted soil and ligninolytic fungal strains were screened and a...

  • Article
  • Open Access
4 Citations
4,422 Views
23 Pages

Survey of Fungal Foliar and Panicle Diseases in Smallholder Sorghum Cropping Systems in Different Agro-Ecologies of Lower Eastern Kenya

  • Irene Njeri Koima,
  • Dora Chao Kilalo,
  • Charles O. Orek,
  • John Maina Wagacha and
  • Evans N. Nyaboga

Sorghum is a staple food crop and plays a critical role in subsistence farming in Kenya due to its adaptability to marginal agro-ecological zones. However, fungal diseases are among the major biotic constraints of sorghum production, causing over 70%...

of 41