Search Results (9,147)

Poly(lactic acid) (PLA) films containing two different hemp-derived essential oils (EOs), Carmagnola CS (Carm) and Futura 75 (Fut), at 1, 5, and 10% wt were successfully produced via solvent casting for packaging applications. The influence of EO presence, type, and concentration on the chemical, morphological, and thermal properties of the PLA-based films was investigated. In addition, radical-scavenging activity, water transport properties, and antimicrobial performance were evaluated to assess the effect of EOs on the structural and functional characteristics of the resulting packaging materials. FTIR spectroscopy confirmed the successful incorporation of the hemp essential oils Carm and Fut into the polymer matrix, with a concentration-dependent effect that is more pronounced for Fut than for Carm. In the second heating run, evaluated by DSC measurements, both EOs lowered T_g from 60.3 °C (PLA) to 52.0 °C for PLA_10 Carm and 55.1 °C for PLA_10 Fut. The EOs act as plasticizers in the PLA matrix, improving the deformation at break. Gas barrier measurements showed that permeability decreased from 3027 ± 300 Barrer (PLA) to (2499 ± 44) Barrer in PLA_10 Carm and 2623 ± 130 Barrer in PLA_10 Fut, with a corresponding reduction in diffusivity. The barrier improvement factor reached 17% for Carm and 15% for Fut, confirming the enhanced barrier performance of PLA_EOs films. DPPH assays showed that PLA_EOs films retained most of the antioxidant activity of the free oils, with only a 10–15% reduction for PLA_Fut and no significant loss for PLA_Carm after one week. After one month, the activity of Carm in PLA film decreased by 18%, whereas the performance of its free form remained unchanged, confirming the superior and more stable radical scavenging capacity of Carm compared to Fut. Overall, the study demonstrates that hemp essential oils can be effectively integrated into PLA without compromising structural integrity, while preserving antioxidant performance and enhancing water barrier properties, supporting their potential as sustainable active packaging components. Full article

Bacillus subtilis is aerobic or facultatively anaerobic. After entering the gastrointestinal tract, its spores germinate and colonize the gut, inhibiting the growth of harmful aerobic bacteria (Escherichia coli, Streptococcus, Staphylococcus aureus). However, it remains unclear whether B. subtilis can inhibit Clostridium perfringens type A infection. In this study, B. subtilis PB6 was added to the diets of pregnant sows infected with Clostridium perfringens type A, which significantly improved the reproductive performance and reduced the incidence of bloat in sows and diarrhea in neonatal piglets. The treatment significantly increased the abundance of intestinal probiotics (B. subtilis, Lactobacillus, Limosilactobacillus reuteri, Lactobacillus johnsonii, Muribaculaceae, Lactobacillus amylovorus, and Lactobacillus reuteri) in sows and decreased the relative abundance of Clostridium perfringens type A after feeding B. subtilis administration. These probiotics can repair the intestinal tissue and improve intestinal histomorphology, and enhance the expression of MUC2 and sIgA in sows, thereby further strengthening the mucosal immune function. B. subtilis can also reduce the levels of inflammatory factors (CRP, IL-1β, and IFN-γ) and attenuate the inflammatory response in sows and neonatal piglets. Taken together, our results suggest that dietary supplementation with B. subtilis PB6 could reduce bloat in sows and diarrhea in piglets while improving intestinal barrier function and microbial balance in sows. Full article

It is shown that transient trapped surfaces form in a class of emerging globally hyperbolic spacetimes, within punctured Planck-scale neighbourhoods of the geon supported on intersecting singular supports whose intersection forms a characteristic core in a non-strongly causal setting. These neighbourhoods shrink towards the intersecting singular support in the distributional geometry. In particular, the trapped surfaces occur near the characteristic limit corresponding to the unstable equilibrium of the self-gravitating geon. They act as an effective classical barrier for descriptions formulated purely within smooth differential geometry. The area of these trapped-surface configurations, computed on Planck-referenced neighbourhoods, is shown to tend to zero both in the asymptotically flat limit of the emerging spacetime and in the geon limit. Thus, transient trapped surfaces evaporate in the sense that their area vanishes as classical and asymptotically flat spacetime emerges within the quantum foam framework. A state-counting generating function for the transient trapped surfaces is constructed from the coherent-state density operator. This generating function maps microscopic occupation-number sectors to macroscopic data and thereby allows a definition of Boltzmann entropy (not to be confused with the von Neumann entropy, which is zero for any pure coherent state). Since the coherent state is constructed to implement the correspondence principle, expectation values of the relevant quantised observables reproduce their classical values. In particular, the expectation value of the bosonic occupation-number operator serves as a microstate-counting variable in the coherent sector. The generating function takes the form of an exponential of this expectation value, leading to an entropy–area relation consistent with the Hawking–Bekenstein scaling. Full article

Rotary kiln-based activated carbon production combines high-temperature operation with flammable/reducing gases, carbonaceous dust, and downstream off-gas treatment and acid/base washing, creating complex escalation pathways. This study prioritizes safety improvements by applying classical failure modes and effects analysis (FMEA) and a transparent Fuzzy-FMEA framework to 18 representative failure modes (six each for kiln/activation, acid/base handling, and atmosphere/control). Five experts evaluated Severity, Occurrence, and Detection on a 10-point scale. The fuzzy model used triangular membership functions (L/M/H), a monotonic 27-rule base, Mamdani max–min inference, and centroid defuzzification to compute a continuous fuzzy risk priority number (FRPN, 0–10). Classical FMEA identified dust explosion (RPN = 405), temperature control failure (RPN = 378), and off-gas leakage (RPN = 324) as the highest-ranked risks. Fuzzy-FMEA preserved the top-risk group while more strongly highlighting barrier-related risks, placing off-gas leakage, instrumentation/interlock failure, and electrostatic ignition control alongside dust explosion (FRPN 9.221–9.332). The rankings were strongly correlated (Spearman ρ = 0.871; Kendall τ = 0.752), yet mid-risk items were rearranged (mean |Δrank| = 2.06; max = 5), improving discrimination within tied RPN clusters. The five highest-priority scenarios were reconstructed into actionable engineering packages, including dust and ignition control, off-gas integrity linked to shutdown logic, interlock proof testing and bypass management, and independent protection layers for kiln temperature control. Full article

Zearalenone (ZEN) is a widely distributed estrogenic mycotoxin that compromises intestinal health in pigs, but its spatial difference ZEN and niche-specific regulatory effects on the intestinal microbiota remain largely unelucidated. In this study, 12 healthy three-way crossbred weaned piglets (Duroc × Landrace × Yorkshire) were randomly divided into two treatments. The control group (CON) was fed with the basal diet, and the treatment group (ZEN) was supplemented with 1.5 mg ZEA/kg of the basal diet for 28 days. Chyme and mucosal microorganisms in the duodenum, jejunum, ileum, colon and cecum were profiled by using 16S rDNA sequencing. The results indicated that ZEN significantly reduced the α-diversity of ileal chyme, while the abnormal increase in α-diversity of ileal and cecal mucosa represented a pathological signature of intestinal mucosal barrier damage induced by ZEN, which was detrimental to intestinal health. β-Diversity analysis revealed ZEN altered the microbial community composition of the cecal chyme. LEfSe analysis revealed gut segment-specific and niche-specific biomarker taxa among the groups, and functional prediction further indicated that ZEN exposure significantly perturbed key metabolic pathways: it downregulated nicotinate and nicotinamide metabolism as well as the citrate cycle in ileal chyme and upregulated the pentose and glucuronate interconversions pathway in cecal chyme. Collectively, this study demonstrated the effects of ZEN on the intestinal microbiota across spatial difference and ecological niches in weaned piglets, providing a basis for elucidating the microecological mechanisms underlying ZEN-induced intestinal injury in pigs. Full article

This study investigates the effects of organic salts, including sodium citrate (SC), calcium citrate (CC), and calcium lactate (CL), on the structure–property–function relationships of thermoplastic starch/poly(butylene adipate-co-terephthalate) (TPS/PBAT) films for active packaging applications. TPS incorporated with organic salts was prepared via twin-screw extrusion, blended with PBAT, and further processed into blown films. The films were systematically characterized using ¹H NMR, FTIR, and SEM, together with optical, mechanical, water vapor permeability, and antimicrobial evaluations against Staphylococcus aureus. The results revealed that SC primarily modulated hydrogen-bonding interactions within the starch matrix, resulting in improved structural homogeneity, balanced mechanical properties, and the highest antimicrobial activity among all formulations. In contrast, CL and CC promoted ionic crosslinking through Ca²⁺–starch interactions, leading to increased stiffness and Young’s modulus but reduced polymer chain mobility and limited release of active species, particularly in CC-containing systems. These differences in molecular interactions were consistent with variations in film microstructure, where SC-containing films exhibited more uniform morphologies, while calcium-based systems showed denser but less permeable structures. Furthermore, films containing SC and CL at appropriate concentrations achieved a favorable balance between transparency, water vapor barrier properties, and antimicrobial performance. Overall, this study provides new mechanistic insights into how monovalent and divalent organic salts govern intermolecular interactions, microstructure, and functional performance in TPS/PBAT systems. The findings highlight the critical role of additive type and concentration in designing biodegradable active packaging materials with tunable mechanical, barrier, and antimicrobial properties. Full article

Chryseobacterium indologenes MA9 is a causative agent of root rot disease in Panax notoginseng (P. notoginseng), with its high incidence being a major manifestation of continuous cropping barriers, severely hindering the sustainable development of the P. notoginseng industry. In this study, a novel lytic bacteriophage, MA9V-3, was isolated from wastewater, targeting C. indologenes MA9. The phage produced clear plaques, ranging from 1 to 3 mm in diameter, with a surrounding halo. Phage MA9V-3 achieved an adsorption rate of up to 80% after 30 min of contact with C. indologenes MA9, a latent period of approximately 40 min, and an average burst-size if 160 PFU/cell. Transmission electron microscopy revealed that phage MA9V-3 possesses an icosahedral head and a contractile tail, exhibiting a typical myovirus-like morphology. According to the latest ICTV taxonomy, MA9V-3 belongs to the class Caudoviricetes, and the phage’s biocontrol efficacy and inhibitory capacity were evaluated at different multiplicity of infection (MOI s). The results showed that the highest titer recorded at 1.6 × 10¹⁰ PFU/mL. Whole-genome sequencing revealed that MA9V-3 is a double-stranded circular DNA virus, with a genome length of 103,203 bp, GC content of 34.29%, and 150 open reading frames (ORFs), one of which is related to tRNA. Only 13 of these ORFs encode known functional sequences, likely due to the limited available gene data for such phages in the database, with additional details on hypothetical proteins yet to be uncovered. Comparative database analysis confirmed that the phage genome contains no antibiotic resistance or toxin-related genes. Phage therapy experiments were performed using MA9V-3 and two other phages screened in our laboratory. The experimental results showed that phage MA9V-3 may be a potential candidate for effectively controlling the infection of Panax notoginseng by C. indologenes MA9, and offering valuable insights into the potential application of phage therapy for managing bacterial plant diseases. Full article

Skin aging is a complex biological process triggered by intrinsic and extrinsic factors, causing structural and functional deterioration, and its mitigation is a priority in cosmetology and functional food science. Skin fibroblasts, which mediate skin repair, wound healing and inflammation, are closely associated with aging. Sea cucumber collagen peptides exhibit prominent anti-aging, immunomodulatory and antioxidant properties, yet their mechanisms in ameliorating skin aging remain elusive, necessitating further exploration. This study verified the anti-skin aging efficacy of sea cucumber collagen peptides in D-galactose-induced aging mice, and explored whether the mechanism involves regulating endoplasmic reticulum (ER) stress in skin fibroblasts. Aging mice were gavaged with sea cucumber collagen peptides; skin moisture, barrier function and hydroxyproline content were measured, and skin morphology was observed. Immunofluorescence and Western Blot were used to detect ER stress-related proteins. Results showed that sea cucumber collagen peptides significantly improved aging mouse skin barrier function, elevated water and collagen fiber contents, and ameliorated the status of fibroblasts and prickle cells. The underlying mechanism may involve inhibiting ER stress in skin fibroblasts and enhancing prickle cell function. These findings confirm the peptides’ high bioavailability and potential as anti-aging functional food ingredients, providing insights for skin aging prevention. Full article

The use of marginal sedimentary aggregates in pavement construction remains a major challenge in mountainous regions due to their high porosity, weak lamination planes, and susceptibility to moisture-induced deterioration. This study investigates the potential of low-density polyethylene (LDPE) plastic waste to enhance the engineering performance of laminated Miocene soft rock aggregates used in bituminous concrete. Aggregates sourced from the Surma Group (Bhuban Formation) in Mizoram, India, were characterized through physico-mechanical, geochemical, and mineralogical analyses to evaluate their durability and moisture sensitivity. X-ray fluorescence (XRF) analysis revealed elevated feldspar and total alkali contents (≈5.15%), indicating a mineralogical composition prone to hydrophilic behavior and stripping within bituminous mixtures. To mitigate these limitations, aggregates were coated with varying proportions of LDPE plastic using the dry process. An optimum LDPE content of 9% by weight of aggregate produced significant improvements in aggregate performance, resulting in a 70.03% reduction in Aggregate Impact Value (from 17.72% to 5.31%), a decrease in Los Angeles Abrasion Value from 42.93% to 31.45%, and an 89.82% reduction in water absorption (from 4.52% to 0.46%). The polymer coating effectively sealed lamination planes and reduced moisture ingress within the sedimentary structure. Bituminous concrete mixtures incorporating LDPE were further evaluated using Marshall stability and indirect tensile strength tests. The addition of 1.1% LDPE by weight of mix significantly enhanced moisture resistance. For mixtures with nominal maximum aggregate sizes (NMASs) of 13 mm and 19 mm, the Tensile Strength Ratio (TSR) increased from 52.59% and 58.58% in the control mixtures to 82.81% and 87.10%, respectively, thereby satisfying the minimum requirement of 80% specified by MoRTH. The results indicate that LDPE functions as a hydrophobic barrier and structural sealant that improves binder–aggregate adhesion and prevents stripping along weak lamination planes. The findings demonstrate that LDPE-modified bituminous concrete provides a sustainable and technically viable strategy for upgrading marginal sedimentary aggregates into durable pavement materials while simultaneously promoting the beneficial reuse of plastic waste. Full article

The food packaging industry requires sustainable solutions to reduce plastic waste and replace synthetic additives. This study addresses the need for scalable methods to transform conventional polyethylene terephthalate (PET) packaging into active food preservation systems using natural biocides. Commercial PET packaging was surface-activated using industrial-scale corona treatment, followed by coating with natural biocides—carvacrol (CV) and trans-cinnamaldehyde (tCN). The resulting active packaging materials (PET-CV and PET-tCN) were characterized using XRD, FTIR, SEM, AFM, and desorption kinetics. Packaging properties including mechanical strength, oxygen barrier, antioxidant (DPPH), and antibacterial activity (against S. aureus and E. coli) were evaluated. Real-food preservation tests were conducted using fresh minced pork (4 °C, 6 days) and table olives (23 °C, 21 days), monitoring microbiological (TVC), colorimetric (CIE L*a*b*), and pH changes. Corona treatment successfully anchored both biocides through physical adsorption, with tCN exhibiting stronger surface interaction (desorption energy: 128.0 kJ/mol). Both coatings significantly improved oxygen barrier properties (61% reduction for PET-CV, 80% for PET-tCN). PET-tCN demonstrated superior antibacterial activity (inhibition zones: 15.0 mm against E. coli). In pork preservation, PET-tCN achieved a 2-log reduction in TVC, maintained meat redness (a*: 12.80 vs. 5.10 for control), and stabilized pH. For olives, PET-tCN reduced TVC by 2.35 log cycles and preserved green color. This corona-assisted coating approach, demonstrated here at laboratory scale, successfully transforms inert PET into multi-functional active packaging with potent antimicrobial, antioxidant, and barrier properties, significantly extending food shelf-life and offering a sustainable solution for reducing food waste. Full article

Introduction: Olives have been used in traditional Mediterranean medicine for thousands of years to address the causes of inflammation, ageing and cognitive health. Traditional preparations of olive include olive oil and olive leaf extract, which are major components of diets that contribute to maintaining cognitive function and reducing neurodegenerative disease risk. Aims of the study: This systematic review aimed to synthesise experimental and limited human evidence on olive biophenols in neurodegenerative disease models, identify the most studied compounds, characterise their mechanisms of action, and evaluate key translational barriers. Materials and methods: Following PRISMA 2020 guidelines and registered with PROSPERO (CRD420251252252), primary studies investigating the effects of well-characterised olive biophenols in neurodegenerative relevant in vitro, in vivo, or human models were systematically reviewed. Each study was assessed for its design, experimental model, mechanistic outcomes and reported limitations. Risk of bias was evaluated using validated tools (SYRCLE/OHAT/ToxR) appropriate for preclinical and experimental study designs. Results: Among the 25 studies, 7 (28.0%) examined oleuropein or oleuropein aglycone, 10 (40.0%) focused on hydroxytyrosol or its derivatives, and 9 (36.0%) investigated oleocanthal. Most studies employed in vivo animal models (57.7%), predominantly transgenic mouse models of AD and toxin-induced PD models. Oleuropein-based studies reported inhibition of amyloid-β and α-synuclein aggregation with behavioural improvements. Hydroxytyrosol primarily exerted antioxidant and anti-inflammatory effects with modest cognitive benefits. Oleocanthal showed the most consistent anti-amyloid and anti-tau activity, including enhanced amyloid-β clearance across the blood–brain barrier. Most studies show a moderate risk of bias due to incomplete reporting, randomisation and blinding. Conclusions: Olive biophenols demonstrate consistent neuroprotective effects in preclinical models; however, translation to clinical application remains limited by pharmacokinetic constraints, methodological heterogeneity, and insufficient human evidence. Full article

Semen parameters, including sperm counts, have rapidly declined in men across the globe over the last five decades. Although this decline remains unexplained, lifestyle factors may affect male fertility. Recently, several studies highlighted a potential link between non-steroidal anti-inflammatory drug (NSAID) usage, such as naproxen and ibuprofen, and declining male fertility. However, the mechanisms by which these common analgesics affect male fertility, including their effects on the blood–testis barrier (BTB), remain poorly characterized. Utilizing an in vitro rhesus macaque non-human primate (NHP) BTB model, we demonstrate that serum levels of naproxen and ibuprofen alter the function of BTB. Following short-term naproxen and ibuprofen treatment of NHP primary Sertoli cells, we show that these NSAIDs increase the transepithelial electrical resistance, indicating an overall strengthening of the Sertoli cell junctions. Furthermore, naproxen and ibuprofen treatment alter the expression of genes involved in maintaining the BTB. Specifically, the genes that were significantly expressed in response to ibuprofen exposure were enriched for human phenotypic abnormalities linked to male factor infertility. Together, these results suggest that short-term naproxen and ibuprofen treatment disrupt the function of the BTB by altering the integrity of the Sertoli cell junctions, proposing a potential role of NSAIDs in male factor infertility. Full article

Chronic inflammatory skin diseases, including atopic dermatitis (AD) and psoriasis, are systemic immune-mediated disorders driven by dysregulated immune responses. The gut–skin axis is a bidirectional network linking intestinal microorganisms, their metabolites, and host immunity. It connects microbiome composition and function with systemic inflammation and cutaneous pathology, shaping disease-specific mechanisms such as Th2/IL-4/IL-13-mediated barrier dysfunction in AD and Th17/IL-23/IL-17-driven hyperproliferation in psoriasis. Microbiota-derived metabolites, including short-chain fatty acids, tryptophan-derived aryl hydrocarbon receptor ligands, and bile acid-dependent FXR/TGR5 signaling, modulate immune homeostasis and epithelial integrity. Gut dysbiosis, impaired metabolite production, and barrier dysfunction disrupt regulatory networks, amplifying inflammation. Microbiota-targeted interventions, including probiotics, synbiotics, postbiotics, and precision nutrition, may serve as adjunctive therapies, although further well-controlled clinical studies are needed. Integrating multi-omics, metabolomics, and functional microbial profiling, alongside investigations of the gut mycobiome and virome, will be critical to identify predictive biomarkers and optimize therapeutic strategies. These concepts remain mechanistically compelling but largely theoretical, requiring validation in longitudinal and interventional studies. Full article

Aiming at the problems of difficult hard-constraint enforcement and weak environmental generalization ability in the safe trajectory planning of manipulators in complex environments, a Policy-Guided Model Predictive Path Integral (PG-MPPI) planning framework is proposed. This framework integrates the advantages of reinforcement learning and model predictive control to construct a global prior guidance, local real-time optimization and hard-constraint safety assurance: a Constraint-Discounted Soft Actor–Critic (CD-SAC) offline learning policy is designed, which incorporates the configuration-space distance field as a safety guidance term to realize the learning of obstacle avoidance behavior; the offline policy is used to guide the online sampling and optimization of MPPI, improving sampling efficiency and planning quality; and a Control Barrier Function (CBF) safety filter is introduced to revise control commands in real time, ensuring the strict satisfaction of constraints. Taking the SIASUN T12B manipulator as the research object, simulation comparison experiments are carried out in multi-obstacle scenarios. The results show that the PG-MPPI algorithm outperforms the comparison algorithms in the success rate of collision-free target reaching, ensures the smoothness and feasibility of the trajectory, and has a good adaptive capacity to complex environments with unknown obstacle configurations, thus providing an efficient solution for the autonomous and safe operation of manipulators. Full article

In an era of rapid urbanisation and climate challenges, understanding how urban land patterns contribute to resilience is crucial for sustainable development. This theoretical review introduces a novel framework positing that greater heterogeneity in plot sizes and land uses enhances urban resilience by promoting the long-term preservation of built environments, multifunctional spaces, and socio-cultural adaptability. Drawing on urban morphology, assemblage theory, and resilience science, we argue that fragmented ownership in small-plot fabrics acts as a barrier to large-scale redevelopment, fostering diversity that buffers against shocks. Through comparative case studies of Venice (Italy), Tokyo (Japan), Hong Kong, Mexico City (Mexico), and York (UK), we illustrate how historical small-plot subdivisions have endured centuries, supporting ecological, economic, and social sustainability. The analysis reveals common patterns: ownership fragmentation preserves fine-grained urban forms, enabling adaptive reuse (exaptation) and inclusivity. The five case studies serve an illustrative function, demonstrating how the theoretical linkages between plot heterogeneity, institutional friction, incremental transformation, and long-term resilience outcomes can plausibly operate in real-world historic urban fabrics. This paper addresses a gap in the literature by synthesising plot-level heterogeneity with broader resilience outcomes, offering policy implications for protecting such fabrics amid global urbanisation pressures. The findings align with land system science, emphasising multifunctionality for regenerative urbanism. Full article