Search Results (190)

Camelina sativa (CS) is an emerging sustainable oilseed crop with interesting feed application potentialities. The research assessed the potentiality of Camelina sativa (CS) in the diet for free-range laying hens, aiming at reaching a nutritional claim. To this purpose, two feeding groups of hens (n = 100 Livorno hens, n = 25 hens/pen) were farmed outdoor and received either a Control diet, which was a commercial diet for laying hens, or a CS diet. The latter was formulated to include the 5% CS cake and 1% CS oil, replacing conventional feedstuffs. Diets were isoprotein and isoenergy and were available ad libitum throughout the laying period (February–September). At day 1, n = 12 eggs/diet were sampled to assess the initial proximate composition and FA profile. Every 7 days the sampling was repeated to analyze the eggs’ FA profile, up to day 35. At the end of the laying season, n = 6 hens/dietary treatment were slaughtered and subjected to meat quality evaluations. Results highlighted that a 28-day feeding was the period required to obtain 227 mg of C18:3 n-3 and 81 mg of C20:5 n-3 + C22:6 n-3/100 g egg, whereas a further 7 days of feeding trial were ineffective in further enhancing the omega-3 FA content of eggs. CS eggs were comparable to Control ones for overall physical traits, proximate composition, and shelf-life. In addition, at the end of the laying period, the meat of CS hens was also found to be healthier than that of Control ones, thanks to a higher omega-3 FA proportion (p < 0.01), which was, however, not sufficient to reach any nutritional claim. Instead, the proximate composition of CS meat was overall comparable to Control hens. In conclusion, the present research demonstrated that feeding Camelina sativa meal and oil to laying hens is feasible and allows to reach the nutritional claim in eggs “rich in omega-3 FA” after a feeding period of 28 days, without any negative effects on other eggs’ quality trials. Camelina sativa can thus be defined as a promising sustainable feedstuff for the poultry sector for diversification purposes and to enhance the nutritional quality of eggs. Full article

Background: Reliable and non-invasive methods for assessing welfare in poultry are essential for improving evidence-based welfare monitoring and advancing management practices in commercial production systems. The iris-to-pupil (IP) ratio, previously validated by our group in primates and cattle, reflects autonomic nervous system balance and may serve as a physiological indicator of stress in laying hens. This study evaluated the utility of the IP ratio under field conditions across diverse commercial layer housing systems. Materials and Methods: In total, 296 laying hens (Lohmann Brown, n = 269; White Leghorn, n = 27) were studied across four locations in Canada housed under different systems: Guelph (indoor; pen), Spring Island (outdoor and scratch; organic), Ottawa (outdoor, indoor and scratch; free-range), and Toronto (outdoor and hobby; free-range). High-resolution photographs of the eye were taken under ambient lighting. Light intensity was measured using the light meter app. The IP ratio was calculated using NIH ImageJ software (Version 1.54p). Statistical analysis included one-way ANOVA and linear regression using GraphPad Prism (Version 5). Results: Birds housed outdoors had the highest IP ratios, followed by those in scratch systems, while indoor and pen-housed birds had the lowest IP ratios (p < 0.001). Subgroup analyses of birds in Ottawa and Spring Island farms confirmed significantly higher IP ratios in outdoor environments compared to indoor and scratch systems (p < 0.001). The IP ratio correlated weakly with ambient light intensity (r² = 0.25) and age (r² = 0.05), indicating minimal influence of these variables. Although White Leghorn hens showed lower IP ratios than Lohmann Browns, this difference was confounded by housing type; all White Leghorns were housed in pens. Thus, housing system but not breed was the primary driver of IP variation. Conclusions: The IP ratio is a robust, non-invasive physiological marker of welfare assessment in laying hens, sensitive to housing environment but minimally influenced by light or age. Its potential for integration with digital imaging technologies supports its use in scalable welfare assessment protocols. Full article

Grazing is a free-range farming model commonly practiced in low-external-input agricultural systems. The widespread use of veterinary antibiotics in livestock farming has led to significant environmental accumulation of antibiotic residues and antibiotic resistance genes (ARGs), posing global health risks. This study investigated the antibiotic residues, bacterial community, ARG profiles, and mobile genetic elements (MGEs) in cattle feces from three provinces in western China (Ningxia, Xinjiang, and Inner Mongolia) under grazing modes. The HPLC-MS detection showed that the concentration of tetracycline antibiotics was the highest in all three provinces. Correlation analysis revealed a significant negative correlation between antibiotic residues and the diversity and population abundance of intestinal microbiota. However, the abundance of ARGs was directly proportional to antibiotic residues. Then, the Sankey analysis revealed that the ARGs in the cattle fecal samples were concentrated in 15 human pathogenic bacteria (HPB) species, with 9 of these species harboring multiple drug resistance genes. Metagenomic sequencing revealed that carbapenemase-resistant genes (bla_KPC and bla_VIM) were also present in considerable abundance, accounting for about 10% of the total ARGs detected in three provinces. Notably, Klebsiella pneumoniae strains carrying bla_CTX-M-55 were detected, which had a possibility of IncFII plasmids harboring transposons and IS19, indicating the risk of horizontal transfer of ARGs. This study significantly advances the understanding of the impact of antibiotic residues on the fecal microbiota composition and ARG profiles in grazing cattle from northwestern China. Furthermore, it provides critical insights for the development of rational antibiotic usage strategies and comprehensive public health risk assessments. Full article

Cage-free egg production is now the predominant system in Australia. However, the occurrence of floor eggs (FE), which are eggs laid outside designated nest boxes, presents a major challenge for these producers. To understand factors that may be associated with the laying of FE, a national scoping survey of cage-free egg-laying flocks was undertaken. Forty-three flocks across multiple farms were surveyed via a phone-based interview using predetermined questions. Floor egg levels ranged from 0.01–17%. There was no difference in floor egg levels between the breeds of brown-egg-laying hens. Age at peak lay did not alter the level of FE, but higher rate of peak lay had a weak association with fewer FE (r = −0.31, p = 0.049). Larger flocks had a lower percentage of FE (r = −0.5, p = 0.002), and farmers of larger sized flocks considered a lower level of floor eggs to be acceptable. Farms with tunnel-ventilated sheds reported fewer FE compared to those using other ventilation systems (p = 0.013). Higher floor egg levels were associated with increased labor costs (p = 0.023). These findings suggest that shed design and environmental management may be leveraged to reduce floor egg occurrence and improve operational efficiency in cage-free systems. Full article

Regarding the trout microbiota, most information is focused on lactic acid bacteria, which can show beneficial properties. However, in trout farming, mostly pathogenic Gram-positive species were reported, such as Staphylococcus aureus, Listeria monocytogenes, and/or Clostridium spp. In this study, free-living trout were analyzed for Gram-negative microbiota that can cause loss as disease-stimulating agents. Bacteriocin postbiotics should be one of the approaches used to eliminate these agents. In total, 21 strains of different species isolated from the intestinal tract of 50 trout in Slovakia (Salmo trutta and Salmo gairdnerii) were taxonomically allotted into 13 species and 9 genera. This method showed variability in microbiota identified using MALDI-TOF mass spectrometry with the following species: Acinetobacter calcoaceticus, Citrobacter gillenii, Citrobacter freundii, Escherichia coli, Hafnia alvei, Kluyvera cryocrescens, K. intermedia, Leclercia adecarboxylata, Raoultella ornithinolytica, Pseudomonas fragi, Ps. putida, Ps. lundensis, Ps. teatrolens, and Serratia fonticola. Most strains were susceptible to the antibiotics used, reaching inhibitory zones up to 29 mm. On the other hand, 3 out of 21 strains (14%) were susceptible to nine enterocins- postbiotics (Hafnia alvei Hal281, Pseudomonas putida Pp391, and Ps. fragi Pf 284), with inhibitory activity in the range of 100–6400 AU/mL. Full article

Background: Short food supply chains are commonly perceived as more sustainable and safer alternatives to conventional production systems, often linked to organic, free-range livestock practices. Materials and methods: This study investigates, for the first time, the distribution of antimicrobial resistance genes (ARGs) and characterizes the microbial communities’ composition, using 16S rRNA sequencing and real-time PCR, respectively. Eleven fecal, 76 slaughterhouse surface, 11 cecal, and 11 carcass samples, from 11 poultry farms belonging to the same short food chain, were analyzed in the study. Results: While cleaning and disinfection procedures appeared to reduce the bacterial load on slaughterhouse surfaces, diverse and potentially resistant bacteria, including genera such as Staphylococcus and Streptococcus, persisted both before and after slaughter. ARGs conferring resistance to high-priority critically important antimicrobials (HPCIAs), such as fluoroquinolones and third-generation cephalosporins, were frequently detected on carcasses, with qnrS (76.15%, 95%CI 68.02-84.28%) and bla_CMY2 (57.8%, 95%CI 48.38-67.22%) being the most prevalent. The slaughtering process emerged as a critical step for ARG dissemination via intestinal bacteria, such as genus Lactobacillus. Additionally, the detection of mcr genes and bla_NDM on carcasses but not in the bird gut samples suggests possible anthropogenic contamination. Discussion: These findings highlight that the evisceration process, slaughterhouse environment, and personnel are all contributing factors in ARG spread and underscore the need for enhanced hygiene protocols and reduced gut ARG carriage in domestic birds to mitigate the risk for the consumer. Full article

A study was conducted in Sierra Leone to identify cassava plants that are asymptomatic and symptomatic to cassava mosaic disease (CMD) and collect planting materials for field trial establishment; determine the prevalence of CMD caused by African cassava mosaic virus (ACMV) and East African cassava mosaic virus (EACMV) using the Nuru App and virus indexing techniques; and assess selected agronomic traits in cassava. A total of 80 cassava farms spanning four provinces (Southern, Eastern, Northern, and North-West) were surveyed in April 2022. Findings showed that the cassava variants of the experiment and locations significantly (p < 0.001) affected CMD incidence, severity, growth, and fresh storage root yield traits. The CMD incidence (87.0%) and whitefly abundance (144.8) were highest, and the CMD severity was moderate (4.0) for the plants derived from cuttings obtained from symptomatic Cocoa mother plants, while plants derived from cuttings of improved mother plants exhibited no visible symptoms of the disease and the lowest population (45.1) of whiteflies. The Nuru app is inefficient for phenotypically detecting CMD at 3 months after planting (MAP), while at 6, 9 and 12 MAP, the app efficiently detected the disease using a molecular analysis technique. Resistant, non-diseased plants derived from cuttings obtained from SLICASS 4 mother plants produced the highest fresh storage root yield (54.9 t ha⁻¹). The highest storage root yield loss was recorded in the plants obtained from cuttings of symptomatic variety Cocoa mother plants harvested at Matotoka grassland ecology, Bombali District (90.2%), while those harvested from cuttings of asymptomatic variety Cocoa mother plants grown at the four test environments had a similar storage root yield loss ranging from 40.3 to 46.2%. Findings suggest the importance of genetic variability, environmental adaptation, utilization of diseased-free materials, and phytosanitation as disease management strategies for increased production. These findings provide important insights into the distribution, impact, and spread of CMD and whitefly abundance in the studied areas in Sierra Leone that could be exploited for cassava production, productivity, conservation, and population improvement. Full article

Background: The use of antibiotics in beekeeping has potential implications for honeybee health and environmental contamination. Recent research indicates that extensive antibiotic use in beekeeping, especially oxytetracycline, promotes antimicrobial resistance in bee-related bacteria. Honeybees can transport oxytetracycline-resistance genes during foraging, potentially establishing reservoirs of resistance in the colony and facilitating intergeneric gene transfer among various gut bacteria as well as in the microbiome of the flowers and the wider environment, where honeybees can spread antibiotic-resistance genes over a large distance. This study investigates the effects of oxytetracycline hydrochloride (OTC) treatment on honeybees from a One Health perspective, examining antibiotic residues in honey, environmental spread, and the presence of tetracycline-resistance genes (TET-RGs). Methods: In the spring of 2022, two groups of four honeybee hives were placed near an almond grove in Central Italy. One group was treated with 1.68 g of OTC, while the other remained untreated. Samples were collected from bees, honey, hive entrances, and flowers before treatment and at 3 as well as 9 days post-treatment. OTC residues and TET-RGs were analyzed to assess contamination and resistance gene dissemination. Results: OTC residues were detected in honey from both treated (day 3: 263,250.0 ± 100,854.3 µg/kg; day 9: 132,600 ± 146,753.9 µg/kg) and untreated hives (day 3: 20.5 ± 8.2 µg/kg; day 9: 135.8 ± 198.6 µg/kg), suggesting cross-contamination. Residues were also found in almond tree flowers (0.7 ± 0.1 µg/kg), with TET-RGs (tet(K), tet(L), tet(M), tet(B), tet(O), tet(D)) detected pre- and post-treatment. In honeybee gut bacteria, resistance genes (tet(M), tet(A), tet(D), tet(B)) appeared post-treatment in both groups. No significant correlation was observed between hive distance and resistance gene presence in flowers, although the presence of other farms located within the bees’ flight range, in which OTC might have been used in the past, could have influenced the results. Conclusions: These findings highlight the risk of OTC-induced antibiotic cross-contamination and the spread of TET-RG, raising concerns for bee health and environmental safety. Given honeybees’ social nature and the negative effects of antibiotics on their health, an antibiotic-free management approach is recommended for sustainable apiculture. Full article

Free-range egg production plays a key role in the global food system, and current market trends suggest that consumer demand for free-range eggs will continue to rise. Free-range egg production is susceptible to a wide range of factors, including climatic conditions, management practices, and disease presence. These factors can cause variability in the laying rate of a flock over time, leading to fluctuations in egg production. The main purpose of this study was to investigate the risk of short-term free-range egg production losses using data derived from a combination of sensing technologies and management activities. Production and environmental data were collected from a commercial farm comprising seven flocks of laying hens. The variables studied included laying rate, feed intake, water intake, solar radiation, humidity, precipitation, and indoor/outdoor temperature. These were processed into a set of aggregate features calculated across a 14-day moving window. Generalized estimating equations were used to analyze the association between the derived production and environmental features and the probability of a short-term drop in egg production, expressed through deviations in the laying rate on the day immediately following the data window. Odds ratios were used to express the relative risk of a production drop by comparing the features for window periods where production drops occur to the window periods where production drops did not occur. The results demonstrated that a range of data features based on the laying rate, feed intake, water intake, and indoor/outdoor temperatures all had significant associations with the odds of a production drop. Key findings from the study show that an increase in feed intake and laying rate measured across the 14-day data window were correlated with a lower risk of a sudden drop in egg production. Conversely, a low mean indoor temperature (x < 16.1 °C group), measured through environmental sensing data, was correlated with a higher risk of a sudden drop in egg production. This study quantifies the link between data features derived from production and environmental monitoring and egg production issues, thereby providing useful insights on the most important data items captured through day-to-day monitoring, which can be used for proactive management. Further research should be carried out to investigate how technologies such as machine learning and analytics platforms can be applied for the task of forecasting production interruptions using the data features explored in this study. Full article

In commercial poultry farming, automated behavioral monitoring systems hold significant potential for optimizing production efficiency and improving welfare outcomes at scale. The behavioral detection of free-range broilers matters for precision farming and animal welfare. Current research often focuses on either behavior detection or individual tracking, with few studies exploring their connection. To continuously track broiler behaviors, the Only Detect Broilers Once (ODBO) method is proposed by linking behaviors with identity information. This method has a behavior detector, an individual Tracker, and a Connector. First, by integrating SimAM, WIOU, and DIOU-NMS into YOLOv8m, the high-performance YOLOv8-BeCS detector is created. It boosts P by 6.3% and AP by 3.4% compared to the original detector. Second, the designed Connector, based on the tracking-by-detection structure, transforms the tracking task, combining broiler tracking and behavior recognition. Tests on sort-series trackers show HOTA, MOTA, and IDF1 increase by 27.66%, 28%, and 27.96%, respectively, after adding the Connector. Fine-tuning experiments verify the model’s generalization. The results show this method outperforms others in accuracy, generalization, and convergence speed, providing an effective method for monitoring individual broiler behaviors. In addition, the system’s ability to simultaneously monitor individual bird welfare indicators and group dynamics could enable data-driven decisions in commercial poultry farming management. Full article

Hepatitis E viruses (HEV) cause hepatitis E in humans. In industrialized countries, sporadic HEV infections, typically caused by HEV genotypes 3 or 4, can become chronic and progress to liver cirrhosis in immunocompromised individuals. Pigs are a significant animal reservoir, implicating raw or undercooked pork products as potential sources of human infection. To better understand HEV dissemination in the Belgian pig population, potential risk factors were investigated by linking farm-level HEV serological status to biosecurity questionnaire data. Farrow-to-finish herd type, free-range systems, and poor boot hygiene were significantly associated with higher within-herd prevalences. This enabled an initial risk profiling of various farming types and the development of predictions for all Belgian pig farms. When combined with the census of the Belgian wild boar population, the predicted HEV status of all professional Belgian pig farms (based on these associations) does not suggest that the proximity of wild boars is a main source of HEV in free-ranging herds. Identifying risk factors for increased circulation of HEV between and within pig farms is critical to controlling its spread and reducing human infection. Full article

After milking, dairy cows seek the nearest water trough, but access depends on competitive success. This study descriptively characterized individual drinking behavior after milking in association with physiological traits and competitive success in a herd of 42 lactating Brown Swiss cows held in a free-range barn. Cows were milked and fed twice a day, and the drinking behavior was video recorded after leaving the milking parlor during 22 milkings in July 2022. Water consumption and 33 behavioral parameters were recorded. Competitive success was defined by an index calculation. Milk production traits correlated positively with the number of trough visits, the total duration of drinking, the duration of water intake and drinking breaks, and the water volume consumed. High-yielding dairy cows exhibited twice as many agonistic interactions as low-yielding ones, suggesting that performance-based grouping increases aggressive interactions. Further, subtle dominance interactions, such as “staring”, comprise a major part of confrontations at the trough. Cows with low competitive success more frequently pursued other activities before they drank and were more frequently interrupted by agonistic interactions. Considering body and performance traits in association with individual drinking patterns in evaluating on-farm water management seemed valuable, therefore, a review of current recommendations for water supply is suggested. Full article

Sheep-associated malignant catarrhal fever (SA-MCF) is a severe lymphoproliferative vascular disease of cattle that is caused by ovine gammaherpesvirus 2 (OvGHV2), which is a Macavirus within the Gammaherpesvirinae subfamily. SA-MCF occurs worldwide in several mammalian hosts. Alternatively, alcelaphine gammaherpesvirus 1 (AlGHV1) is a Macavirus that causes wildebeest-associated malignant catarrhal fever (MCF), which principally occurs in cattle from Africa. Previous serological assays to evaluate the presence of MCF in mammals used a competitive inhibition enzyme-linked immunosorbent assay (CI-ELISA). This CI-ELISA is based on the 15A antigenic epitope that is common to all Macavirus associated with the development of MCF in their respective hosts. This study evaluated an indirect MCF-specific ELISA assay based on the AlGHV1 C500 strain to detect antibodies against OvGHV2 in 43 closed dairy cattle farms from Southern Brazil. These farms are located in a region where subclinical infections by OvGHV2 have been detected in free-ranging wild boars (Sus scrofa). Sheep or goats were not reared at these farms or within the proximity of these farms. Risk factors associated with seropositivity to OvGHV2 were evaluated, while the possible participation of subclinically infected wild boars in the dissemination of OvGHV2 was estimated using spatial analysis. Sera from 29 dairy cows from 16 farms demonstrated sample/positive (S/P) values considered positive with this MCF-specific ELISA (cutoff S/P, 0.063). The S/P values for the positive dairy cows varied between 0.0633 and 0.2510 (mean, 0.0998; standard deviation, 0.0476). At least one cow was seropositive in 16/43 (37.2%) of these farms, with seropositivity identified in 29/367 (7.9%) of dairy cows maintained at these farms. Additionally, dairy cows raised within the intensive system had a more than threefold higher chance of being seropositive to OvGHV2 relative to those reared within the semi-intensive system. Furthermore, the spatial evaluation revealed that cows on dairy farms within a 50 km radius of the home range of subclinically infected wild boars had an increased risk of being seropositive to this assay. These findings demonstrated that the AlGHV1 C500-specific MCF ELISA can be efficiently used to monitor the occurrence of OvGHV2 in cattle. In addition, the occurrence of subclinically infected free-ranging wild boars within a radius of 50 km from susceptible cattle may be a possible risk factor for the occurrence of OvGHV2-related infections in these animals from Southern Brazil. These initial results are fundamental to understanding the epidemiology of OvGHV2-associated infections and clinical SA-MCF in mammals in Brazil. Full article

Giant freshwater prawn (Macrobrachium rosenbergii) farming in Bangladesh began in the 1970s and has become a significant export industry. Despite its potential, there are concerns about the environmental sustainability of prawn farming due to its high greenhouse gas (GHG) footprint, but implementation of integrated multi-trophic aquaculture (IMTA) may help minimize the GHG emission. A key element in IMTA is using plants to take up inorganic nutrients released by the prawns, producing valuable plant products and cleaning the water. Using a quadrat sampling method, we conducted a field study in combined prawn and shrimp ponds, aquaculture fishponds, and non-aquaculture waters in south- west Bangladesh to characterize plant diversity and identify suitable species for IMTA in prawn farms. A total of 38 plant species were identified with densities ranging from 4.5–6.1 plants/m² in the aquaculture ponds to 11.6–17.1 plants/m² in the prawn/shrimp and the non-aquaculture ponds. Free-floating plants were the most abundant, followed by emergent, floating anchored, and submerged plants. Most plants have commercial values as food, fodder, fish feed, fertilizer, or medicines to local people. Our results suggest that species within the Oxalis, Ipomoea, Azolla, and Lemna genera are suitable extractive aquatic plants for the implementation of IMTA in prawn farms and may improve the sustainability of prawn production. Full article

With the aim to characterise the situation of the subsector, 25 poultry farms of the endangered native Utrerana chicken egg-laying-oriented breed (Gallus gallus Linnaeus, 1758) were surveyed in Andalusia (southern Spain) from 2021 to 2023 to investigate the structure of the farms, number of birds, health status, feeding management, and marketing of their products. It was found that the pace of foundation of Utrerana chicken farms accelerated from 2009, and most of the farms were concentrated in the province of Seville. Only 40% of the farms were legally registered. This breed is raised on small farms mainly dedicated to self-supply and raising birds for conformation and their aesthetic values, with limited marketing of eggs and meat, with facilities consisting often of one poultry house and one outdoor enclosure, and with a low number of laying hens per farm (53.5 on average). Current productivity is low, with an average of 139 eggs per hen per year, but hens have a long reproductive lifespan, averaging 4 years. The Partridge variety of the breed was the most common, followed by the Black-barred and Black varieties, while the White variety was very scarce, with only 31 specimens in total. Two-thirds of the farmers also raised other breeds of chickens, in addition to the Utrerana. The management and production system was free-range or backyard, with a certain proximity to the requirements of European Union-certified organic production with regard to stocking densities and generally with a good sanitary status, respect for welfare, and implementation of biosecurity measures on the farms. The feeding of the chickens was based on grazing in outdoor enclosures and on the provision of grains and compound feed, with 40.0% of farmers using commercial compound feed and half preparing feed themselves. Two-thirds of the farmers were affiliated with the National Association of Utrerana Chicken Breeders (“Asociación Nacional de Criadores de Gallinas Utreranas”, ANCGU, in Spanish); almost half of them took part with their birds in fairs and conformation shows, such as the Utrerana Chicken Fair held in Utrera (province of Seville) and more than a quarter of them have sold live birds of the breed at these fairs. The farmers carry out few promotional and advertising activities for their farms and products, predominantly by using social networks, and the market area reached is mainly local. In conclusion, this research has shown that the current alternative production system for Utrerana chicken is similar to that of the hens that originated the breed and consists of a few amateur farms, with a small number of birds, distributed mainly throughout the Andalusian countryside. It has been highlighted that there is a need for the livestock authorities to implement programmes for supporting farmers and promoting the breeding of this endangered avian breed, native to the Spanish zoogenetic heritage. Full article