Search Results (1,816)

(1) Background: Subclinical neck pain is mild-to-moderate neck pain that has not yet been treated, and where individuals experience pain-free days. Alterations in sensorimotor integration, motor control, proprioception, and cerebellar inhibition have been observed in individuals with subclinical neck pain. Upregulation of the cervico-ocular reflex is documented in subclinical neck pain, with no difference in the gain of the vestibulo-ocular reflex. Vestibulo-ocular reflex gain adaptation and associated differences in visuo-motor control have not been successfully measured in this population. This study aims to investigate the vestibulo-ocular reflex gain adaptation and visuo-motor control in individuals with subclinical neck pain. (2) Methods: 30 right-hand-dominant participants (19 healthy controls: 10 male and 9 female; 16 subclinical neck pain: 6 male and 10 female) aged 18 to 35 performed an eye tracking task. Participants were seated 90cm away from a monitor and instructed to hold their gaze on a stationary or moving target projected onto a screen while performing active head rotations. Trials were divided into 12 blocks (pre-adaptation, 10 adaptation, and post-adaptation) for a total of 192 trials. During adaptation, the target would move at increasing speeds during each block, increasing by 10% of active head velocity up to a maximum of 100%. (3) Results: The subclinical neck pain group demonstrated significantly higher total saccades (p = 0.006, ƞ² = 0.240) and overt catch-up saccades (p = 0.041, ƞ² = 0.141) than the healthy control group. (4) Conclusion: Subclinical neck pain alters the visual–vestibular interaction. Full article

Background/Objectives: Conventional next-generation sequencing (NGS) workflows often require more than two weeks to complete, delaying treatment decisions and limiting access to precision oncology in community settings. This report aimed to demonstrate the feasibility of performing rapid, comprehensive cell-free DNA (cfDNA)-based genomic profiling by introducing a fully automated NGS workflow in a community hospital environment. Case Presentation: A postoperative patient with pancreatic ductal adenocarcinoma and liver metastasis underwent cfDNA-based liquid biopsy using plasma collected in PAXgene^® Blood ccfDNA Tubes. Gene analysis was performed using the Oncomine Precision Assay GX5 on the Ion Torrent Genexus™ System (Thermo Fisher Scientific). Three pathogenic hotspot mutations—KRAS G12R, TP53 M246I/M246K, and GNA11—and one copy number gain in PIK3CA were identified, whereas no variants were detected in a healthy volunteer control. The total turnaround time from plasma separation to report generation was approximately 27 h, requiring only 40 min of total hands-on time. Discussion: This rapid, automated workflow enabled comprehensive cfDNA analysis within a clinically practical timeframe, overcoming key limitations of conventional multi-step NGS workflows that typically require external sample shipment and specialized personnel. The results confirm the technical feasibility of conducting high-quality molecular testing in a regional hospital setting. Conclusions: This report demonstrates that fully automated cfDNA-based NGS can achieve clinically meaningful genomic profiling within 27 h in a community hospital. This advancement addresses the time and cost barriers of traditional NGS analysis and represents a significant step toward promoting precision medicine in community healthcare. Full article

Unmanned aerial vehicle (UAV) tracking faces significant challenges due to small targets and background interference. Traditional anchor-based tracking algorithms require designing numerous proposals to capture such tiny targets, which entails unacceptable computational overhead. On the other hand, anchor-free tracking methods struggle to adapt to target scale variations, resulting in suboptimal tracking accuracy in anti-UAV tracking scenarios. To address these limitations, we pioneer the integration of diffusion models into visual tracking, proposing SiamDiff—a scale-adaptive anti-UAV framework. We reformulate the tracking task as a bounding box prediction problem, where a diffusion model is leveraged to generate scale-adaptive proposals. Furthermore, we propose a Learnable Mask Module (LMM) and a Frequency Channel Fusion Module (FCFM) to enhance discriminative feature extraction for small targets. Additionally, we design a Scale-Aware Diffusion Strategy (SADA) to boost robustness to scale variations. Experimental results on the Anti-UAV and Anti-UAV410 benchmarks demonstrate the effectiveness of our approach, achieving a State Accuracy (SA) of 71.90% and 67.03%, respectively, outperforming the baseline and other trackers. Moreover, our method shows superior adaptability to scale variations, confirming its robustness in complex anti-UAV tracking scenarios. Full article

This paper introduces a lightweight and accessible interaction system for treadmill-based video games, relying solely on facial tracking via a smartphone’s front camera. The system enables real-time estimation of running cadence and directional control through natural head movements, providing an immersive and hands-free gaming experience. A key contribution is the implementation of a FFT-based cadence estimation method that achieves accuracy errors below 5% using only 128 frames, enabling real-time feedback. Preliminary evaluations with 11 participants demonstrate that the FFT-based approach outperforms traditional peak detection in both accuracy and robustness across multiple running speeds. These results position the system as a practical, efficient, and scalable solution for fitness-oriented human–computer interaction, with promising implications for digital health and exergaming. Full article

TEMPO derivatives are well known as scorch retardants due to their ability to effectively quench free alkyl radicals during peroxide crosslinking of polymer compositions. However, in practice this leads to the loss of crosslinking density due to a irreversible decrease in the number of alkyl radicals involved in the crosslinking process. One approach to solving this problem is the use of TEMPO-based biradical molecules, which, on the one hand, are able to effectively quench alkyl radicals, and on the other hand, can couple macroradicals, partially compensating for the loss of crosslinking density. The aim of this work was to reveal the effect of bis(1-oxyl-2,2,6,6-tetramethylpiperidin-4-yl) sebacate (bis-TEMPO) in the concentration range of 0.11–0.44 phr on the delay in the onset of dynamic crosslinking of polyolefin composites initiated by peroxide, as well as the oxidative stability of the resulting crosslinked composites. The obtained data show that using bis-TEMPO at a concentration of less than 0.27 phr increases the crosslinking density of the polyolefin composite, with a crosslinking onset delay of up to 36 s achieved. Simultaneously, antioxidant functionality of bis-TEMPO in crosslinked composites is considered moderate and leads to an increase in the OIT values by 1.7–2.8 times. The crosslinking onset delay time under dynamic conditions is well described by a first-order kinetic model at a constant temperature. The obtained data confirm the efficiency and predictability of bis-TEMPO as a scorch retardant for polyolefin composites. Full article

Background: Covering the defects around the calcaneus is still a largely debatable subject. In the classical view, the defects at the level of the foot can be treated only by a free flap. In a modern approach, it has been observed that for small or moderate foot defects, a local flap can be used. Methodology: In this case series, we have retrospectively selected the patients who were admitted to the orthopedic department for a calcaneal fracture and who presented soft-tissue complications during the treatment. The patients have been selected from the past five years if they have undergone reconstructive surgery with a local or regional flap. Results: By applying the inclusion and exclusion criteria, we found that out of 79 patients who have been admitted to the orthopedic department, only two patients met the criteria. Two flaps have been used to treat the defects that developed at the level of the calcaneus after traumatic injury of the foot. The reverse-flow sural flap, as a tunneled flap, had a good evolution, without vascular suffering of the flap. On the other hand, for defects at the medial level of the calcaneus, we have used the dorsalis pedis flap. The healing was fast, and the patient presented no complications at the level of the donor site. Conclusions: Both flaps presented a good evolution. We try to emphasize through this article that soft tissue defects around the non-weight-bearing area of the heel can also be treated through a non-microsurgical option. These two options can help the ortho-plastic team to manage difficult cases by avoiding a free flap or a split-thickness skin graft. Full article

The metabolism of polyunsaturated fatty acids (PUFAs) is a broad research field, and the products identified so far offer potential medical and industrial applications. Epoxy fatty acids (EpFAs) act as lipid mediators that modulate renal function, angiogenesis, vascular dilatation and inflammation; moreover, they regulate monocyte aggregation and are involved in cardiovascular and metabolic diseases. On the other hand, EpFAs are precursors of environmentally friendly products for the plastics industry, in which the grade of epoxidation of the compounds gives the polymeric material different advantageous characteristics. The controlled chemical synthesis of poly epoxidized PUFAs is challenging as the reactions are non-selective. In contrast, the biosynthetic route based on cytochrome P450 monooxygenases and lipoxygenases is highly selective but ineffective due to the instability of the enzymes in cell-free systems. Fungal unspecific peroxygenases (UPOs, EC 1.11.2.1) with P450-like activity offer a suitable alternative for the selective synthesis of EpFAs from PUFAs. Here we demonstrate that a recombinant unspecific peroxygenase from Aspergillus niger (rAniUPO) is able to sequentially epoxidize eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) to 14,15-17,18 diepoxyeicosatrienoic acid (14,15-17,18 diEpETrE) and 16,17-19,20-diepoxydocosatetraenoic acid (16,17-19,20 diEpDTE), respectively, while arachidonic acid is transformed into 13-hydroxy-14,15-epoxyeicosatrienoic acid (14,15-hepoxilin B₃). Optimal production for these oxygenated derivatives (up to 15 mg) was achieved using 2 mM hydrogen peroxide as the co-substrate. The obtained molecules were identified using high-resolution mass spectrometry and their structure was verified by NMR. Our results demonstrate the suitability of UPOs for the synthesis of EpFAs that can be used in medical research and industrial applications. Full article

Accurate and real-time detection of polyps in colonoscopy is a critical task for the early prevention of colorectal cancer. The primary difficulties include insufficient extraction of multi-scale contextual cues for polyps of different sizes, inefficient fusion of multi-level features, and a reliance on hand-crafted anchor priors that require extensive tuning and compromise generalization performance. Therefore, we introduce a one-stage anchor-free detector that achieves state-of-the-art accuracy whilst running in real-time on a GTX 1080-Ti GPU workstation. Specifically, to enrich contextual information across a wide spectrum, our Cross-Stage Pyramid Pooling module efficiently aggregates multi-scale contexts through cascaded pooling and cross-stage partial connections. Subsequently, to achieve a robust equilibrium between low-level spatial details and high-level semantics, our Weighted Bidirectional Feature Pyramid Network adaptively integrates features across all scales using learnable channel-wise weights. Furthermore, by reconceptualizing detection as a direct point-to-boundary regression task, our anchor-free head obviates the dependency on hand-tuned priors. This regression is supervised by a Scale-invariant Distance with Aspect-ratio IoU loss, substantially improving localization accuracy for polyps of diverse morphologies. Comprehensive experiments on a large dataset comprising 103,469 colonoscopy frames substantiate the superiority of our method, achieving 98.8% mAP@0.5 and 82.5% mAP@0.5:0.95 at 35.8 FPS. Our method outperforms widely used CNN-based models (e.g., EfficientDet, YOLO series) and recent Transformer-based competitors (e.g., Adamixer, HDETR), demonstrating its potential for clinical application. Full article

Background/Objectives: The stability of the gingival margin after crown lengthening is a key determinant of esthetic success in anterior rehabilitation. Digital workflows have been proposed to improve surgical precision, but their long-term impact on marginal stability remains insufficiently substantiated. Methods: A total of 87 patients (622 maxillary anterior and premolar teeth) who underwent esthetic crown lengthening were retrospectively evaluated. Patients were allocated to either a digitally guided workflow (G1, n = 62) or a freehand approach (G2, n = 25). Gingival margin stability was assessed using standardized intraoral photography and calibrated digital measurements at baseline, 1–2 months, and 12 months. Recurrence, periodontal parameters, complications, and patient satisfaction (VAS) were recorded. Mixed-effects models accounted for the clustering of teeth within patients. Results: Both workflows achieved predictable crown lengthening outcomes. At 12 months, median gingival margin recurrence was significantly lower in the guided group (G1: 0.14 mm [IQR 0.10–0.19]) compared with the freehand group (G2: 0.27 mm [IQR 0.20–0.34]) (p < 0.001). Secondary surgical revision was required in 1.6% of G1 patients versus 16.0% of G2 patients (p < 0.001). Patient satisfaction was high overall but greater in G1 (mean VAS 9.65 ± 0.52) than in G2 (8.96 ± 0.73). No major biological complications occurred. Conclusions: Digitally guided crown lengthening resulted in improved gingival margin stability and reduced the need for secondary correction compared with the freehand approach. Precise control of the bone crest position relative to the planned gingival margin is critical for long-term esthetic success. Full article

A series of dimethylpyridine-3-carboxamide derivatives was designed as potential, selective, non-zinc chelating inhibitors of matrix metalloproteinase 13 (MMP-13), and subsequently synthesized. The identity of the obtained compounds was confirmed by FT-IR, ¹H/¹³C NMR, and HR-MS methods. Fluorescence spectroscopy was applied to study the interaction of synthesized compounds with human serum albumin, providing insight into their potential transport properties in plasma. In parallel, the electronic properties and reactivity parameters relevant to enzyme binding of the designed molecules were analyzed using density functional theory. Molecular docking and molecular dynamics simulations revealed the compounds to interact preferentially and stably within the S₁ pocket of MMP-13 via hydrogen bonds and π-stacking interactions. The calculated binding free energy confirmed the stability and persistence of the complexes during simulation, indicating a strong and specific recognition pattern. On the other hand, their affinity towards MMP-8 was considerably weaker, which is consistent with the predicted selectivity profile. In addition, the biological evaluation confirmed MMP-13 inhibition. Finally, in vitro tests revealed their cytotoxic activity against cancer cell lines. Full article

Current upper-limb rehabilitation robots primarily focus on training tasks involving free movements or static interactions with rigid objects. These paradigms lack simulation of complex object manipulation tasks encountered in daily life, thereby limiting the training of patients’ high-level sensorimotor integration capabilities. To address this gap, this study proposes an innovative robotic rehabilitation training system designed for functional occupational therapy. Specifically, the task of transporting a water cup was abstracted into a cup–ball system integrated with a robotic arm. The ball was modeled as a point mass, and kinematic and dynamic analyses of the system were conducted. A visual tracking method was employed to monitor the ball’s motion and calculate its slosh angle. Owing to the impaired fine motor control in stroke patients, a sloshing suppression control strategy integrating exponential filtering, feedforward force compensation, and impedance control was proposed to prevent the ball from spilling. Experiments validated the effectiveness of the proposed method. The results indicated that with full compensation, the oscillation rate of the ball was significantly reduced, and the smoothness of the hand force was markedly improved. This study presents an effective method for addressing dynamic uncertainty in rehabilitation robot training, thus significantly improving the functional relevance of the training. Full article

Taraxacum mongolicum Hand.-Mazz. and Sonchus oleraceus L. are perennial herbaceous species with strong antioxidant capacities; however, these species have not been widely utilized as medicinal materials because their natural populations are limited, and manual harvesting is labor-intensive. In this study, we compared the yield, bioactive components (in leaves and roots), and antioxidant capacity of domesticated and wild populations of both species. Domesticated cultivation significantly increased the contents of ascorbic acid, total phenols, and flavonoids in T. mongolicum leaves, total phenols in T. mongolicum roots, and total phenols and flavonoids in S. oleraceus leaves. Domestication markedly enhanced antioxidant capacity (with the exception of S. oleraceus roots) and the free radical scavenging capacity in the leaves and roots of both wild vegetable species (with the exception of S. oleraceus roots). Domesticated cultivation also substantially improved yield, with T. mongolicum yield increasing by 12,850 kg/ha (fresh weight) and S. oleraceus yield increasing by 18,600 kg/ha (fresh weight). Correlation analysis showed that the free radical scavenging capacity of leaves in both species was significantly negatively correlated with the soluble sugar content, whereas the soluble sugar content in roots was significantly positively correlated with the soluble protein content. Overall, our findings will aid further studies of bioactive substances and antioxidant-related genes in T. mongolicum and S. oleraceus. Full article

Cantaloupe melon seeds are a byproduct that can be upcycled for their nutritional value, generating added value, reducing food waste, and supporting food sustainability. This study evaluated the effects of melon seed flour on selected physicochemical and consumer acceptance of gluten-free cookies. Melon seeds were dehydrated at 60 °C for 12 h and ground. Then gluten-free cookies containing varying melon seed flour (20, 40, 60, 80, and 100%) were prepared by mixing the ingredients and baked at 177 °C for 18 min. Color, water activity, proximate composition, and mineral contents of the melon seed flour were measured. Color, water activity, spread factor, and hardness of the five cookie formulations were evaluated. Finally, a randomized block design was used for the consumer test with 90 consumers. Appearance, aroma, flavor, texture, grittiness, and overall liking were evaluated using a 9-point hedonic scale. Also, purchase intent was asked for before and after a sustainability claim. Data were analyzed using an ANOVA and the post hoc Tukey test (p < 0.05). The McNemar test was used to test whether there were significant differences in purchase intent before and after a sustainability claim. Melon seed flour had 21.4% protein, 34.93% crude fiber, 3% ash, 4% moisture, and 26.9% fat. Spread factor and a* (color redness) values increased with increasing melon seed flour. On the other hand, the more melon seed flour in cookies, the lower the L* value and water activity. The treatment with 40% melon seed flour had the highest liking score, 6.25. Finally, the sustainability claim significantly increased the positive purchase intent of the cookies. This study demonstrates the potential of cantaloupe melon seed flour as an ingredient in food, such as gluten-free cookies. This practice in the food industry can help increase value and reduce waste in cantaloupe processing. Full article

Background/Objectives: Guided endodontic microsurgery is a novel approach designed to improve safety and precision compared with conventional freehand techniques. The present study aimed to evaluate the accuracy, stability, and operative time of trephinations performed using stereolithographic surgical guides designed with Blue Sky Plan and Exoplan software compared with the conventional technique. Methods: A comparative in vitro study was conducted on 72 roots from 12 stereolithographic mandibles. Cone-beam computed tomography (CBCT) and intraoral scans were superimposed to design 16 surgical guides with verification windows and metallic sleeves. Trephinations were performed using a control freehand group, Blue Sky Plan, and Exoplan. Stability, accuracy, and operative time were assessed. Statistical analysis included ANOVA, Kruskal–Wallis, and chi-square tests. Results: Exoplan demonstrated superior accuracy (0.17 mm deviation), stability (12.5% failures vs. 50% in Blue Sky Plan), and shortest operative time (106 s vs. 127 s and 155 s). Differences were statistically significant (p < 0.05). Operative accuracy was independent of surgical duration. Conclusions: Exoplan outperformed Blue Sky Plan and freehand trephinations in stability, accuracy, and efficiency. These findings highlight the potential of digital guides for safer and more predictable endodontic microsurgery. Further clinical validation is required. Full article

Background/Objectives: Screw placement accuracy with robotic guidance shows better results than freehand techniques, yet there is scant data for region-specific outcomes, fusion rates, and complication profiles in different populations. The purpose of this study is to assess and describe screw-rod accuracy, fusion rates, and complications of robot-assisted pedicle screw placement in a Spanish cohort. Methods: Robotic-guided technique for thoracolumbar fusion in 115 patients (July 2020–January 2025) using ExcelsiusGPS^® platform (Globus Medical Inc., Audubon, PA, USA). Inclusion criteria: adults (≥18 years) with postoperative CT to assess accuracy. Primary outcomes were screw accuracy (assessed on the Gertzbein–Robbins scale), fusion rates, and complications (infection, osteolysis, and subsidence). Jamovi 2.6 was employed for statistical analysis. Results: Of a total of 726 screws implanted, 590 screws (95 patients) were studied: 584 (99%) Grade A, 5 (0.8%) Grade B, and 1 (0.2%) Grade C. Fusion was achieved in 85 out of 95 patients (89.5%). Complications included superficial infections (3/115 patients, 2.6%) and asymptomatic osteolysis (8/95 patients, 8.4%, mostly at S1). There was no screw subsidence or removal. Conclusions: Robotic-guided pedicle screw placement demonstrated exceptional accuracy (99% Grade A), high fusion success (89.5%), and minimal complications in this Spanish cohort, supporting its clinical utility for spinal instrumentation. Full article