Search Results (185)

To assess the risk of cross-species transmission of canine parvovirus (CPV) from free-roaming dogs to wild giant pandas in the Foping National Nature Reserve, we collected rectal swabs and serum samples from dogs and fecal samples from giant pandas seasonally (August 2024–August 2025), combined with population surveys and GPS home-range tracking. Vaccination coverage declined from 54.2% to 36.4%, while the proportion of susceptible dogs rose from 8.7% to 29.5%. The CPV nucleic acid positive rate in dogs was 3.9% (4/102, all sub-adults, three deaths), whereas all giant panda samples were negative. Based on individual–seasonal exposure, the serum exposure rate was 30.4%, with immature dogs at significantly higher risk than adults (OR = 5.37). Home-range overlap between dogs and giant pandas (95% KDE: 19.17% in cold seasons vs. 2.61% in warm seasons) and encounter probability were markedly higher in winter and spring. Canine-derived CPV strains possessed the molecular potential to infect giant pandas. In summary, CPV persists long-term in free-roaming dog populations, summer vaccination has not established an effective immune barrier, and winter–spring is a high-risk window for cross-species transmission. We recommend enhanced winter–spring immunization, spatial control measures, and quarantine protocols for incoming dogs. Full article

Free-living unowned cats are prevalent across the UK and may experience numerous welfare challenges. However, there is limited research around this. Cat shelter workers were interviewed on the complexities of unowned cat welfare and management, current barriers and recommended solutions. A total of 25 participants partook in semi-structured one-to-one interviews, which were thematically analysed. Some of the main welfare concerns described were overpopulation, health issues, the absence of preventative medical care, cruelty, and neglect. Key barriers included limited resources, funding, personnel and infrastructure, alongside inappropriate management of cats not suited to domestic lifestyles. External barriers included insufficient government support, limited public awareness around cat welfare issues, and fragmented care provision of cats within the community. Other overlapping barriers include inconsistent terminology use and management practices within shelters and communities. Participants recommended more strategic use of shelter resources towards population management programmes including TNR, professional staff training, and the standardisation of terms such as “stray” and “feral”. They advocated for a shared responsibility framework for unowned cats involving animal welfare organisations, the veterinary sector, communities, and the government. This would include stronger collaboration between charities to share knowledge and establish industry best practice, alongside greater government support through the introduction and enforcement of legislation. Full article

This study investigated the prevalence and molecular characteristics of canine vector-borne pathogens (CVBPs) circulating in diverse dog populations in Thailand by using molecular diagnostic methods. A total of 400 blood samples were collected from four groups (n = 100 each): stray dogs (Group A), vector-borne disease–suspected companion dogs (Group B), healthy companion dogs presenting for routine examination (Group C), and companion dogs presenting with non-vector-borne illnesses (Group D). The overall infection rate was 46.25%. Ehrlichia spp. were the most frequently detected pathogens (23.5%), followed by Babesia spp. (16.5%), Rickettsia spp. (15.0%), and Anaplasma spp. (11.5%). The prevalence differed markedly among groups, including group A (88.0%), group B (54.0%), group C (27.0%) and group D (16.0%) (p < 0.05). DNA sequence analysis showed 100% identity with GenBank™ reference sequences, confirming the presence of Ehrlichia canis, Rickettsia asembonensis, Babesia vogeli, and Anaplasma platys. The detection of CVBPs across all groups demonstrates free-roaming and owned dogs serve as reservoirs for substantial ongoing infections and pose potential zoonotic implications to humans. Overall, these findings emphasize the importance of sustained molecular surveillance, improved vector control strategies, and proactive monitoring of high-risk dog populations to reduce the burden of CVBPs in Thailand. Full article

Feral donkeys (Equus asinus) are well adapted to arid ecosystems and are found in large populations in the deserts of Australia and the Americas. We assessed resource selection and seasonal home range size of female donkeys in southern California between 2020 and 2022 based on telemetry data. We also examined whether dyads with greater encounter rates were more likely to test positive for asinine herpesvirus 5 (AHV-5) and/or Streptococcus equi zooepidemicus (SEZ). Donkey home ranges were non-significantly larger in the cool/wet season (November through March; mean 318.37 ± sd 417.65 km²) than in the hot/dry season (April through October; mean 159.35 ± 212.43 km²). Donkeys selected flatter areas closer to water year-round but selected greater herbaceous cover during the cool/wet season and lower heat loads during the hot/dry season. Individuals testing positive for SEZ selected lower elevations during the wet season and closer distances to water during the dry season; donkeys testing positive for AHV-5 selected areas farther from water during the wet season and steeper slopes during the dry season. The dyad encounter rate was unrelated to presence of either disease. Our results contribute to the understanding of donkey ecology, allowing feral populations to be better controlled by specific and focused management. Full article

Background: Rabies remains a major zoonotic disease worldwide, particularly in regions with large populations of free-roaming animals. In urban settings, animal-related injuries constitute a substantial healthcare burden and frequently result in the administration of rabies post-exposure prophylaxis (PEP). This study aimed to evaluate the epidemiological characteristics of animal exposures and real-world PEP practices in a metropolitan emergency department. Methods: This retrospective descriptive study included 1960 patients presenting to a tertiary metropolitan emergency department between 1 March and 1 September 2025 with suspected animal exposure. Demographic data, animal species involved, exposure mechanisms, animal ownership and vaccination status, time to presentation, and PEP practices were analyzed using descriptive statistics. Results: Most exposures were cat-related (86.3%) and were caused by scratching (81.5%). Nearly all injuries were superficial (99.8%), while deep injuries were rare (0.2%). The majority of animals were classified as strays (90.1%), and vaccination status was unknown in 81.2% of cases. Rabies immunoglobulin was administered to only 0.6% of patients; however, rabies vaccination was initiated in 98.8% of patients. Approximately 74.5% of patients presented within 24 h. Post-exposure animal observation was documented in only 20.2% of cases. Conclusions: Urban animal exposures in this metropolitan setting were predominantly superficial and cat-related, yet nearly all patients received rabies vaccination. Limited animal observation and incomplete vaccination documentation appear to constrain risk stratification and may contribute to the use of precautionary PEP. Strengthening surveillance systems, improving documentation, and implementing evidence-based risk-stratification strategies are essential for optimizing rabies prophylaxis practices in urban environments. Full article

Background: Free-roaming cats in Australian cities contribute to wildlife impacts, community concerns, and high shelter intake. We used an observational pre–post evaluation study design of a targeted cat desexing program (“StrayCare Metro”) delivered with councils and community partners in four local government areas (LGAs) of Greater Sydney (2022–2024). Methods: Program records documented cat enrolments and services; council and state databases supplied annual shelter intake, euthanasia, and cat-related complaints; and transect drives in two LGAs (2021 and 2024) estimated cat encounter rates and population density. The analysis did not include control LGAs. Results: The program desexed 1225 cats; among enrolled cats not already microchipped, 72% received a microchip and 28% declined despite this being offered for free. Compared with pre-program baselines, annual council shelter intake decreased by 49–73% within LGAs (61% overall), with concurrent reductions in euthanasia. Cat-related complaints declined in three LGAs (47–64%) but increased in one. Transect drives indicated substantial declines in cat encounter rates in Blue Mountains (51%) and Campbelltown (35%) and lower density estimates in both surveyed LGAs. Conclusions: A collaborative targeted desexing approach was associated with large reductions in council pound intake, euthanasia, and, in most areas, nuisance complaints, alongside independent indications of reduced free-roaming cat density. Full article

Cats are among the most beloved and affectionate companion animals to humans. Historically, they have been utilised to manage pests or offer comfort and companionship, a practice that continues today. Due to human malpractice, unowned free-roaming cats (as stray pets or feral cats) are now considered amongst the 100 worst invasive species, and are responsible for the decline and even the disappearance of many wild species worldwide. Free-roaming cats maintain their hunting instincts, causing problems for native species, which is recognised as a major issue in island biodiversity. Despite their impact, limited studies have been conducted to understand the spatial activity of free-roaming cats in the Mediterranean when they are away from their caregivers (owners who feed and care for their cats while allowing unrestricted outdoor roaming). To investigate this, we used GPS tracking collars to monitor 15 free-roaming cats on the island of Cyprus, during spring–autumn 2022. The monitored cats were active in a spectrum of different habitats, from forests and farmland to shrublands and the suburbs. We monitored cats for 5.6 days, on average, to investigate their home range sizes (KDE 95%; median: males = 55,678 m²; females = 11,377 m²), daily distance travelled (median: males = 1233 m; females = 538 m), and daily/nocturnal activity, and the factors that influence these patterns. The animals’ sex, shelter availability, and the type of coverage in an area show statistically significant differences in relation to their home range, while activity peaked during the afternoon hours, a finding that is also statistically confirmed. Although the sample size of the study is relatively small, the influence of environmental and anthropogenic factors on the home range of free-roaming cats in Cyprus is revealed. These findings offer quantitative evidence and can contribute to wildlife conservation and free-roaming cat management. Full article

Cats are among the most popular pets globa lly, yet little is known about how the home environment influences their behaviour. Most studies have focused on cats in shelters or research facilities, potentially limiting applicability to pet cats. This study combined behavioural data from cats in three housing conditions: indoor pet (n = 10), free-roaming pet (n = 18), and research (n = 8), collected in summer and winter. Eight behaviours were classified from collar-mounted accelerometer data using a validated machine learning model and analysed using generalised linear mixed models. Free-roaming pet cats were more active in summer than winter (3.9 ± 0.39% vs. 2.7 ± 0.33%; p < 0.001) and more active than both research (2.0 ± 0.36%; p = 0.004) and indoor pet cats (2.0 ± 0.36%; p < 0.001) in summer. Research cats spent more time lying (52.9 ± 2.03% vs. 36.9 ± 2.89%; p = 0.009) and eating (7.8 ± 0.41% vs. 2.4 ± 0.39%; p = 0.003) in winter than summer, whereas no seasonal differences in these behaviours were observed for pet cats. A bimodal daily activity pattern, with peaks around sunrise and sunset, was observed across housing conditions and seasons. These findings demonstrate that both housing and seasonal conditions influence domestic cat behaviour and should be considered when interpreting behavioural studies. Full article

Dogs, especially as pets but also an increasing number of stray dogs, share environments with humans, facilitating the transfer of antibiotic resistance genes (ARGs) between genetic compartments, with zoonotic and public health implications that must be addressed within One Health. In this cross-sectional comparative study, we explored the distribution of seven selected clinically relevant ARGs in both genomic DNA (gDNA) and plasmid DNA (pDNA), and the phenotypic resistance profile of the cultivable microbiota, between pet dogs (PeDs, n = 12) and free-roaming dogs (FRDs, n = 10) in Mexico. Tetracycline resistance genes (tetQ, tetW, and tetM) predominated in both compartments (40% to 100%), suggesting the presence of a core tetracycline-associated resistome. In contrast, plasmid-associated differences were group-specific: in pDNA cfxA was enriched in FRDs (90%) and tetK in PeDs (42%), whereas blaTEM-1 and ermC were absent in two dog populations. Cultivable bacteria from both groups exhibited phenotypic multidrug resistance, particularly by β-lactams, macrolides, lincosamides, and tetracyclines. FRDs also harbored pathogenic–zoonotic bacteria such as Yersinia enterocolitica, Campylobacter jejuni, and Enterococcus faecalis. Our findings indicated that FRDs and PeDs harbor substantial resistomes, with differences in plasmid-associated ARGs, revealing a transfer potential related to environmental exposure. Full article

Background: Microplastics (MPs) and nanoplastics (NPs) are now common in land and water ecosystems. Their spread is an increasing issue from a One Health perspective. These particles end up in soils, water, air, and farm inputs. This poses direct risks to animal health and indirect risks to people who eat animal-derived food. There are also risks from plastic additives and pesticides migrating with these particles in animal-based food. Scope and Approach: This review summarizes how MPs and NPs move in agroecosystems and livestock production. It covers their main sources, such as agricultural plastics, sludge-amended soils, plastic-lined storage, and environmental fallout. It explains how farm animals are exposed, including through feed, water, soil contact, and inhalation. Evidence is condensed for occurrence in manure, tissues, and animal products. The review also highlights key analysis challenges, especially those limiting the assessment of nanoplastic exposure. Key Findings: Field surveys show very different contamination levels in the environment. Agricultural soils range from 0.36 to 42,960 particles/kg. Livestock indicators, like contaminated feed and manure, range from 10² to 10⁵ particles/kg. In free-roaming systems, chicken feces have very high loads, showing trophic transfer in land food chains. A pilot study found plastic particles in pig and cow blood, suggesting some particles cross the gut into the blood. Experimental models link MPs/NPs to oxidative stress, inflammation, mitochondrial dysfunction, metabolic disturbance, and potential reproductive toxicity in livestock and poultry. Conclusions and outlook: Animal-based foods provide a major source of human exposure. MPs and NPs have been observed in milk and poultry products, such as packaged meat and eggs (mean 11.67 ± 3.98 particles/egg). There is still a research gap on raw milk taken directly from the teat and on raw eggs that have not been handled or packaged. This gap makes it hard to identify real contamination sources and control strategies. The review stresses the need for harmonized detection methods (especially for NPs), monitoring from farm to fork, and practical ways to reduce plastic use on farms and minimize contamination during processing, feed handling, and packaging. Full article

This article examines how Turkey’s 2024 amendment to the Animal Protection Law reshapes volunteer caregiving for free-roaming dogs in Istanbul by reconfiguring the practical conditions under which care is sought, coordinated, and sustained. Drawing on 43 in-depth interviews and five months of fieldwork (1 July–30 November 2025), this study combines constructivist grounded theory with reflexive thematic analysis to trace how legal change is encountered through everyday governance interfaces and how these encounters reorganize caregivers’ routines, capacities, and moral worlds. The analysis yields four interlocking findings. First, caregivers describe a temporality of “living in pre-loss,” in which anticipated removal, disappearance, and uncertain outcomes generate chronic vigilance, anticipatory grief, and ambiguous loss without closure. Second, caregiving is increasingly recalibrated as risk management: commitments persist, but intervention narrows through heightened exposure to complaints, reputational scrutiny, and fears that help-seeking may backfire. Third, institutional pathways—hotlines, shelter intake, and municipal responses—are experienced as discretionary and opaque, producing a fluctuating threshold between assistance and harm that conditions whether caregivers engage official systems at all. Fourth, this study identifies a recurring veterinary bottleneck at the street–clinic–recovery handover, where limited short-term holding capacity stalls treatment trajectories and displaces recovery labor into precarious domestic and informal spaces. Together, these findings argue that caregiver well-being is not ancillary to animal welfare governance but constitutive of it. It shapes the continuity of monitoring, the timeliness of intervention, and the everyday mediation through which coexistence is maintained under intensified legal and political pressure. Full article

Free-roaming cats may contribute to zoonotic risk via parasites and other eukaryotic taxa, yet surveillance in protected island settings is limited and conventional coprology can miss low-intensity or degraded signals. We conducted a cross-sectional 18S rRNA metabarcoding survey to establish a baseline profile of potentially pathogenic eukaryotes in community cats from La Graciosa (Natura 2000, Canary Islands, Spain) prior to large-scale antiparasitic interventions. We analysed 152 faecal samples, including fresh samples collected during a high-throughput TNR campaign (n = 37) and dry environmental deposits (n = 115). Host amplification was reduced using a feline 18S blocking primer; libraries were sequenced with Oxford Nanopore technology; and taxonomy was assigned using SILVA-based classifiers with downstream filtering for veterinary/zoonotic relevance. After quality control, 72 eukaryotic taxa were retained and DNA from at least 24 potentially pathogenic taxa was detected. Dipylidium caninum was most frequent (74.3%; 113/152), and opportunistic fungi/yeasts were common (e.g., Pichia kudriavzevii 42.4%, Diutina catenulata 31.5%). Zoonotic protozoa showed low-to-moderate detection frequency (Acanthamoeba castellanii 13.3%, Toxoplasma gondii 7.9%, Balamuthia mandrillaris 4.6%). Overall richness did not differ between fresh and dry samples (p > 0.05), but fresh samples contained higher richness of potentially pathogenic taxa (p < 0.01). Full article

Municipal animal shelters play a crucial role in rescuing abandoned animals, managing free-roaming populations, and promoting animal welfare. This seven-year retrospective study analyzed 2291 animals (1132 dogs, 1159 cats) admitted to a Portuguese municipal shelter (CVM Feira) between 2018 and 2024 to identify predictors of live outcomes. Descriptive and regression analyses were performed on 1325 adoptable animals. Adoption occurred for 58.9% of these animals, while 41.1% remained in long-term housing, reflecting growing shelter capacity pressures. Length of Stay (LOS) was the strongest predictor of adoption, with extended stays markedly reducing adoption odds and contributing to overcrowding. For dogs, female sex and owner or animal protection association (APA) surrender increased adoption likelihood. Microchipped animals were 71 times more likely to be returned to their owners, although only a small proportion entered the shelter with identification. The overall Live Release Rate (LRR) was 79.7% and Save Rate (SR) 84.7%, highlighting effective life-saving efforts, while the Population Balance Calculation (PBC) of 75.6% revealed challenges in population flow-through. These findings provide evidence that data-driven strategies, including reducing LOS, enforcing microchipping, and applying species-specific management, are essential to optimize municipal shelter operations and improve animal welfare, with insights applicable to broader European and international contexts. Full article

Managing community cats on islands requires reconciling animal-welfare mandates with biodiversity protection under real operational constraints. In the Canary Islands (Spain), national Law 7/2023 endorses ethical, non-lethal colony management, while subsequent regional resolutions restrict TNR in and around protected areas, narrowing municipal room for action. We combine a multilevel governance assessment with stochastic demographic simulations parameterized from official records to compare three sterilization regimes over 20 years. The intensive regime (≈60–70%/year) reflects the coverage threshold previously identified by Spain-based modelling and field evaluations and adopted in national program guidance; the 20%/year regime represents the pre-resolution baseline widely observed across the archipelago up to December 2024; and the 4%/year regime reflects the post-resolution reality, with abrupt declines in sterilizations, operations largely confined to urban cores, and program suspensions in multiple municipalities. Minimal (4%) and low (20%) efforts produce rapid population growth, bringing numbers close to the assumed carrying capacity under our deliberately high-K configuration and sustaining high densities and associated welfare and ecological risks; only sustained high-coverage TNR prevents saturation and produces progressive declines across island contexts. Under insular constraints, outcomes are determined by achievable coverage rather than regulatory intent; aligning policy and implementation to secure continuous, high-coverage TNR—particularly in risk-sensitive areas with appropriate safeguards—offers a feasible pathway to meet animal-welfare obligations while limiting ecological pressure. Full article

Free-roaming dogs are a neglected population that poses public health concerns in tourist areas. This study, conducted during both the high and low tourist seasons, aimed to (i) investigate the movement patterns of free-roaming dogs on the Lipe tourist destination island, southern Thailand, focusing on their daily distance, and (ii) identify habitat land type preferences. Between September and December 2024, eight owned and seven unowned dogs were fitted with Catlog GPS devices. Tracking data were collected for the dog’s coordinates every 15 min for at least four consecutive days. Data analysis and visualization were performed using R software. On average, dogs traveled 5300 m daily. Unowned dogs exhibited their highest speed on beaches (median = 70, IQR = 44–79 m per 15 min). Free-roaming dogs spent most of their time in human habitats. The time of day influenced dogs’ appearance across different land types. In conclusion, free-roaming dogs on the island traveled longer distances than in most previous studies. The dogs mostly preferred being around human habitats. Land types influenced dogs’ speed. Dogs appeared on different land types at different times of day. Guided by these data, we recommend (i) better dog registration and other measures to reduce free-roaming activities of dogs and (ii) regular blanket coverage of dog rabies immunization to make the island safer for tourists and residents. Full article