Search Results (17)

The search for sustainable, economically viable, and effective plant protection strategies against pathogenic bacteria, fungi, and viruses is a major challenge in modern agricultural practices. Chitosan (CS) is an abundant cationic natural biopolymer known for its biocompatibility, low toxicity, and antimicrobial properties. Its potential use in agriculture for pathogen control is a promising alternative to traditional chemical fertilisers and pesticides, which raise concerns regarding public health, environmental protection, and pesticide resistance. This study focused on the preparation of chitosan nanoparticles (CS-NPs) through cross-linking with organic molecules, such as tannic acid (TA). Various formulations were explored for the development of stable nanoscale particles having encapsulation capabilities towards low compounds of varying polarity and with potential agricultural applications relevant to plant health and growth. The solution properties of the NPs were assessed using dynamic and electrophoretic light scattering (DLS and ELS); their morphology was observed through atomic force microscopy (AFM), while analytical ultracentrifugation (AUC) measurements provided insights into their molar mass. Their properties proved to be primarily influenced by the concentration of CS, which significantly affected its intrinsic conformation. Additional structural insights were obtained via infrared and UV–Vis spectroscopic measurements, while detailed fluorescence analysis with the use of three different probes, as model cargo molecules, provided information regarding the hydrophobic and hydrophilic microdomains within the particles. Full article

Macroalgal extracts are widely recognised as biostimulants that enhance crop productivity and plant growth under both optimal and stressful conditions. They offer a sustainable approach to mitigating the adverse effects of abiotic stress on crop development. This study investigates the efficacy of macroalgal-based fertilisers in enhancing tomato (Solanum lycopersicum L.) growth, yield, and fruit quality, as sustainable alternatives to chemical fertilisers. Different seaweed species (Sargassum muticum, Ulva ohnoi, Furcellaria lumbricalis, Ascophyllum nodosum, and a commercial A. nodosum extract) were evaluated as foliar treatments. The results showed that while the leaf fresh weight and chlorophyll content were not significantly affected, the fruit morphology and biochemical composition exhibited notable variations. Sargassum muticum-treated fruits displayed the highest °Brix (6.57), indicating superior sugar accumulation, while Ulva ohnoi maintained near-neutral pH levels (avg. 3.94), suggesting balanced acidity. Ascophyllum nodosum extracts induced the highest proline concentrations (peak: 63.77 µmol/g), but also caused extreme acidity (pH 1.39–2.58). Furcellaria lumbricalis enhanced the fruit size (axial length up to 41.4 mm), but reduced the pH sharply (1.69–2.13). The commercial product underperformed in regard to sugar content and flavour complexity. The integrative analysis revealed species-specific flavour profiles: Sargassum yielded sweet, mildly acidic fruits; Ascophyllum produced intensely aromatic, acidic tomatoes; and Ulva resulted in bland flavours. These findings underscore the importance of algal species and extraction methods in tailoring biofertilisers for target fruit qualities. This study advocates for the use of macroalgal fertilisers in sustainable agriculture, but highlights the need for optimised formulations to balance crop yield, taste, and stress adaptation. Full article

Discourses on human embryo experimentation often refer to monozygotic twinning and individuation. A criterion to establish regulations that guide human embryo research proposes that individuation is achieved once the embryo ceases to have the potential for dividing into two or more viable entities at about 15 days of gestational age. This standard is based on an updated version of a developmental model initially proposed by George Corner. A fundamental problem with this approach is the model’s lack of sufficient evidence to explain adequately human embryo twinning and, consequently, to serve as a basis to establish appropriate ethical guidelines for embryo experimentation. In addition, subsequent formulations of Corner’s model added an extension of blastomere totipotency to different moments of gestation, without a proper scientific basis. The model is also challenged by monozygotic twinnings that result in placental and amniotic arrangements incompatible with Corner’s framework. Investigations into the physiology of fertilisation and of the zygote suggest that individuation may occur at a very early stage. An alternative description of monozygotic twinning may explain better sesquizygotic twinning events and serve to re-evaluate the individuation criterion. The study aims to investigate deficiencies in the embryology of this model and assess their ethical implications. Full article

Maize, regarded as a staple economic crop, attracts special global attention with the aim to enhance its production. Foliar fertilisation offers a complementary method to traditional soil fertilisation amongst resource-limited agricultural systems, providing a more efficient solution to nutrient deficiencies, especially in suboptimal soil conditions. This study aimed to analyse foliar fertiliser formulation research directions and their application in maize production. A literature search was conducted in the Web of Science (WoS) database. Bibliometric analyses were performed using the VOSviewer software (version 1.6.17). The changes in the publication trends of documents were tested using the Mann–Kendall test. The production effects of foliar fertilisation were independently synthesised. The results showed a strong positive increase in publication trends regarding maize foliar fertilisation (R² = 0.7842). The predominant nutrients that affected maize production were nitrogen, phosphorous, potassium, zinc, iron, and manganese. The timely foliar application of nutrients corrected deficiencies and/or sustained nutrient supply under several abiotic stresses. Foliar application at critical growth stages like flowering and grain filling boosted carbohydrate and protein content, lipid levels, kernel size, mineral content, and the weight of the maize grain. This review identified important research gaps, namely genotype-specific responses, interactions with other agronomic practices, and long-term environmental effects. Full article

In a scenario of accelerated global climate change, the continuous growth of the world population, and the excessive use of chemical fertiliser, the search for sustainable alternatives for agricultural production is crucial. The present study was conducted to evaluate the plant growth-promoting (PGP) characteristics of two yeast strains, Candida guilliermondii and Rhodotorula mucilaginosa, and the physicochemical characteristics of nanometric capsules and iron oxide nanoparticles (Fe₂O₃-NPs) for the formulation of nanobiofertilisers. The physiological and productive effects were evaluated in a greenhouse assay using lettuce plants. The results showed that C. guilliermondii exhibited higher tricalcium phosphate solubilisation capacity, and R. mucilaginosa had a greater indole-3-acetic acid (IAA) content. The encapsulation of C. guilliermondii in sodium alginate capsules significantly improved the growth, stomatal conductance, and photosynthetic rate of the lettuce plants. Physicochemical characterisation of the Fe₂O₃-NPs revealed a particle size of 304.1 nm and a negative Z-potential, which indicated their stability and suitability for agricultural applications. The incorporation of Fe₂O₃-NPs into the capsules was confirmed by SEM-EDX analysis, which showed the presence of Fe as the main element. In summary, this study highlights the potential of nanobiofertilisers containing yeast strains encapsulated in sodium alginate with Fe₂O₃-NPs to improve plant growth and photosynthetic efficiency as a path toward more sustainable agriculture. Full article

Objective: In this study, we aimed to investigate the effects of facile combinations of biochar and N, P, and K fertilisers on the growth and physiological characteristics of Lithocarpus litseifolius seedlings, and to optimise the biochar/NPK ratio of Lithocarpus litseifolius. Method: A four-factor three-level orthogonal method was used to conduct a field experiment using 2-year-old Lithocarpus litseifolius. Nine fertiliser treatments and one control treatment (CK, i.e., no fertiliser) were established in a completely randomised block group with six replications of ten treatments. The plants were planted in October 2020 and harvested in November 2021, and fertiliser was applied once in April 2021 and once in July 2021. Results: Rational application could effectively promote the growth of Lithocarpus litseifolius, and T4(C₂N₁P₂K₃) produced the highest increase in plant height growth (17.03 cm), diameter growth (5.47 mm), soluble sugar (94.60 mg/g), soluble protein (4.59 mg/g), and phlorizin (old leaf: 16.00%; tender leaf: 15.13%); T8(C₃N₂P₁K₃) resulted in the highest chlorophyll a content (1.46 mg/g), chlorophyll b content (0.62 mg/g), and total chlorophyll content (2.08 mg/g), and T1(C₁N₁P₁K₁) resulted in the highest contents of starch (11.60 mg/g) and trilobatin (old leaf: 0.29%; tender leaf: 2.28%). The indicators corresponding to the above three treatments were significantly higher than those under the other treatments (p < 0.05). The results as analysed by the affiliation function method show that the highest mean value of the affiliation function was 0.645 after T4(C₂N₁P₂K₃) treatment. Conclusions: The combination of biochar and nitrogen, phosphorus, and potassium fertilisers can effectively increase the biomass and active components of Lithocarpus litseifolius while reducing the amount of chemical fertiliser applied. A comprehensive analysis of the results showed that the T4 treatment (biochar: 20 g/plant; urea: 10 g/plant; superphosphate: 9 g/plant; potassium chloride: 12 g/plant) resulted in the highest comprehensive score, with the highest increase in plant height growth, ground diameter growth, root–crown ratio, soluble sugar, soluble protein, and phlorizin, as well as other indicators. Full article

In recent years, the world has witnessed one of the most severe raw material crises ever recorded, with serious repercussions for maintaining its agri-food supply chain. This crisis risks dramatically impacting the poorest areas of the planet and poses profound reflections on global food security. In this complex geopolitical context, the recovery and recycling of renewable resources have become an obligatory path and, today, more than ever, essential in the fertiliser industry. To achieve these objectives, TIMAC AGRO Italia S.p.A. has undertaken a research activity to review the formulation of fertilisers by diversifying the raw materials used and introducing recycled raw materials. This article carried out a life cycle assessment (LCA) on four fertilisers to identify and quantify whether the changes influenced the environmental impacts, highlighting how applying the circular economy within industrial processes can reduce the pressure on natural resources. The results demonstrate that the global warming potential (GWP) impacts of the different reformulated fertilisers show a considerable variation of 4.4–9.2% due to the various raw materials used, the nitrogen content, and related emissions deriving from environmental dispersion. This study shows the importance of the LCA methodology to analyse and quantify the impact categories generated on the life cycle of fertiliser production and to identify the optimal by-products and end-of-waste for the fertiliser industry to find a synergy between environmental and agronomic performance. It also highlights the relevance of the transition to circular production and consumption systems to reduce environmental pressures and their effects on communities and ecosystems without compromising yields. Finally, the positive results encourage accelerating the circular transition and finding alternatives to virgin-mined raw materials. Full article

Micronutrients are essential to plants, and enhancing their availability is one of the agronomic challenges to improving crop quality and yield. This study, under controlled greenhouse conditions, compares tomato plants’ responses to two different micronutrient EDTA-chelated formulations, one of them including a newly developed fulvate–lignosulfonate coating. Growth, yield, and several physiological parameters, including photosynthetic gas exchange, water-use efficiency, leaf nutrient content, leaf greenness and the effective quantum yield of photosystem II, were measured to compare their efficiency. The results showed that the new coated formulation significantly improved growth and most of the determined physiological parameters. At the end of the experiment, higher foliar levels of Fe (2.4-fold) and Mn (2.9-fold) were measured, revealing increased availability of lignofulfonate-complexed micronutrients compared to the traditional fertiliser. Moreover, the photosynthesis rate and stomatal conductance were 9- and 20-fold higher, respectively, than when using the standard fertiliser. In conclusion, the new coated fulvate–lignosulfonated fertiliser provided a more suitable source of micronutrients for tomato plant fertilisation, allowing for higher yields, which correlated with a generally improved physiological response. Full article

Seaweeds represent a diverse and valuable source of cosmetic compounds such as vitamins, minerals, trace elements, amino acids, antioxidants, etc., with moisturizing, anti-inflammatory, and regenerative effects. The so-called “blue cosmetics” represent a line of products related to the use of natural active ingredients and an important market share in major international cosmetic brands. To be recognised as environmentally sustainable, it is essential to ensure that algae-derived products comply with environmentally sound harvesting, production, and extraction practices. In this work, Life Cycle Assessment (LCA) methodology was used to carry out an environmental impact assessment of the processing of the brown algae extract from Fucus vesiculosus and its comparative profile with the most used antioxidants in cosmetics: vitamin C and green tea extracts. Considering an equivalent formulation in antioxidant content, the results showed that seaweed has the lowest environmental load while green tea extracts have the highest environmental impact. Furthermore, to further reduce emissions from seaweed processing, the use of renewable energy sources and the valorisation of biomass residues as fertilisers in a circular economy approach are proposed. Full article

The meat industry generates a large amount of animal by-products not only derived from the slaughter process but also due to the losses and waste of meat products along the supply chain, contributing to the world’s food loss and waste problem. Yearly, 1.7 Mt of meat in the European retail sector and 20% of meat for consumption is wasted in this sector of the supply chain. Therefore, the aim of this paper was to find and evaluate alternatives for the valorisation of agri-food residues, more specifically the meat waste from the food retail sector, through a technological perspective. Thus, we delve into the industrial processes already implemented and the emerging procedures that use muscle, bones and fats by-products from poultry, cattle and pork as the main raw materials in order to identify and characterise them. The results indicate that in addition to the current destinations—landfill, incineration and the rendering process—these animal by-products can be incorporated in the production of biodiesel, food formulations, pharmaceuticals, fertilisers and biogas through an industrial symbiosis approach. Consequently, the several valorisation processes and procedures identified not only suggest an increase in concern about the impacts of the disposal of these materials, but also highlight the potential associated with the use of animal by-products as raw material to obtain added-value products. Full article

Glyphosate-based herbicides (GBH) are the most used herbicides in the world, carrying potentially adverse consequences to the environment and non-target species due to their massive and inadequate use. This study aimed to evaluate the effects of acute exposure to a commercial formulation of glyphosate, Roundup^® Flex (RF), at environmentally relevant and higher concentrations in zebrafish larvae through the assessment of the inflammatory, oxidative stress and cell death response. Transgenic Tg(mpxGFP)i114 and wild-type (WT) zebrafish larvae (72 h post-fertilisation) were exposed to 1, 5, and 10 µg mL⁻¹ of RF (based on the active ingredient concentration) for 4 h 30 min. A concentration of 2.5 µg mL⁻¹ CuSO₄ was used as a positive control. Copper sulphate exposure showed effectiveness in enhancing the inflammatory profile by increasing the number of neutrophils, nitric oxide (NO) levels, reactive oxygen species (ROS), and cell death. None of the RF concentrations tested showed changes in the number of neutrophils and NO. However, the concentration of 10 µg a.i. mL⁻¹ was able to induce an increase in ROS levels and cell death. The activity of antioxidant enzymes (superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx)), the biotransformation activity, the levels of reduced (GSH) and oxidised (GSSG) glutathione, lipid peroxidation (LPO), lactate dehydrogenase (LDH), and acetylcholinesterase (AChE) were similar among groups. Overall, the evidence may suggest toxicological effects are dependent on the concentration of RF, although at concentrations that are not routinely detected in the environment. Additional studies are needed to better understand the underlying molecular mechanisms of this formulation. Full article

Common wheat grains are characterised by low concentrations of Ca, K, and Mg, which can be partially removed with the bran during milling processes. This preliminary study investigated the effects of foliar fertilisation at the earing stage with nitrates of Ca, Mg, and K contemporarily, together with a small amount of urea and protein hydrolysate as potential carriers, in two contrasting common wheat varieties, i.e., Solehio (medium proteins content) and Vivendo (high proteins content). Based on the preliminary grain-to-straw concentration ratio of these minerals, two biofortification targets were applied in order to increase their grain contents by +20% and +40%, in comparison with untreated controls. Here, we demonstrate that the highest fertilisation dose was effective in increasing grain K by 13% and Mg by 16% in Vivendo, and Ca by 7% in Solehio, with no boosting effects of the co-formulants urea and protein hydrolysate. In addition to some qualitative benefits due to nitrates supply, negligible phytotoxicity symptoms were observed, as revealed by the NDVI vegetational index dynamics. Although the biofortification target was not fully achieved, this study firstly reports the possibility to increase at the same time Mg and K, and to a lower extent Ca in wheat grains. It is concluded that efficient multiple biofortification should consider a variety-depend response, while further studies are necessary to investigate the effects of different fertilisation timings and doses for improving the poor mineral translocation to the grains. Full article

Nitrogen fertiliser application represents the largest anthropogenic source of nitrous oxide (N₂O) emissions, and the magnitude of these emissions is dependent on the type of fertilisers applied in the agroecosystems. Despite N-P-K compound fertilisers being commonly used in agricultural soils, a lack of information exists regarding their effects on N₂O emissions. This study aims at examining the effects of different commonly used N-P-K compound fertiliser formulations with contrasting nitrate to ammonium ratios (0.05 to 0.88) on N₂O emissions, yield, and nitrogen use efficiency (NUE) in temperate grassland and to compare these variables with common straight N fertilisers. Compound fertilisers with varying NPK inclusion rates (18-6-12, 10-10-20, 24-2.2-4.5, and 27-2.5-5), and calcium ammonium nitrate (CAN) and urea + N-(n-butyl) thiophosphoric triamide (NBPT) were applied at 80 kg N ha⁻¹ to experimental plots in managed grassland on two occasions in a growing season. Fluxes of N₂O during the experiment period, yield and NUE following two harvests were measured. The cumulative N₂O emission from urea + NBPT, 18-6-12, 10-10-20, and 24-2.2-4.5 treatments were significantly reduced by 44%, 43%, 37%, and 31% compared with CAN treatment under conducive soil moisture condition. Under the same soil condition, 18-6-12 and 10-10-20 treatments showed higher yield, N uptake, and NUE although did not significantly differ from the other fertiliser treatments. Our results suggest that ammonium-based compound fertilisers have a potential to reduce N₂O emissions while maintaining yields. Further long-term study is needed to capture the full magnitude of variations in N₂O emissions, including ammonia (NH₃) volatilization from nitrate and ammonium-based compound fertiliser applications from multiple soil types and under different climatic conditions. Full article

Nutrient-rich organic wastes and soil ameliorants can benefit crop performance and soil health but can also prevent crop nutrient sufficiency or increase greenhouse gas emissions. We hypothesised that nitrogen (N)-rich agricultural waste (poultry litter) amended with sorbents (bentonite clay or biochar) or compost (high C/N ratio) attenuates the concentration of inorganic nitrogen (N) in soil and reduces emissions of nitrous oxide (N₂O). We tested this hypothesis with a field experiment conducted on a commercial sugarcane farm, using in vitro incubations. Treatments received 160 kg N ha⁻¹, either from mineral fertiliser or poultry litter, with additional N (2–60 kg N ha⁻¹) supplied by the sorbents and compost. Crop yield was similar in all N treatments, indicating N sufficiency, with the poultry litter + biochar treatment statistically matching the yield of the no-N control. Confirming our hypothesis, mineral N fertiliser resulted in the highest concentrations of soil inorganic N, followed by poultry litter and the amended poultry formulations. Reflecting the soil inorganic N concentrations, the average N₂O emission factors ranked as per the following: mineral fertiliser 8.02% > poultry litter 6.77% > poultry litter + compost 6.75% > poultry litter + bentonite 5.5% > poultry litter + biochar 3.4%. All emission factors exceeded the IPCC Tier 1 default for managed soils (1%) and the Australian Government default for sugarcane soil (1.25%). Our findings reinforce concerns that current default emissions factors underestimate N₂O emissions. The laboratory incubations broadly matched the field N₂O emissions, indicating that in vitro testing is a cost-effective first step to guide the blending of organic wastes in a way that ensures N sufficiency for crops but minimises N losses. We conclude that suitable sorbent-waste formulations that attenuate N release will advance N efficiency and the circular nutrient economy. Full article

The agricultural sector faces looming challenges including dwindling fertiliser reserves, environmental impacts of conventional soil inputs, and increasingly difficult growing conditions wrought by climate change. Naturally-occurring rocks and minerals may help address these challenges. In this case, we explore opportunities through which the geosphere could support viable agricultural systems, primarily via a literature review supplemented by data analysis and preliminary-scale experimentation. Our objective is to focus on opportunities specifically relating to emerging agricultural challenges. Our findings reveal that a spectrum of common geological materials can assist across four key agricultural challenges: 1. Providing environmentally-sustainable fertiliser deposits especially for the two key elements in food production, nitrogen (via use of slow release N-rich clays), and phosphorus (via recovery of the biomineral struvite) as well as through development of formulations to tap into mineral nutrient reserves underlying croplands. 2. Reducing contamination from farms—using clays, zeolites, and hydroxides to intercept, and potentially recycle nutrients discharged from paddocks. 3. Embedding drought resilience into agricultural landscapes by increasing soil moisture retention (using high surface area minerals including zeolite and smectite), boosting plant availability of drought protective elements (using basalts, smectites, and zeolites), and decreasing soil surface temperature (using reflective smectites, zeolites, and pumices), and 4. mitigating emissions of all three major greenhouse gases—carbon dioxide (using fast-weathering basalts), methane (using iron oxides), and nitrous oxide (using nitrogen-sorbing clays). Drawbacks of increased geological inputs into agricultural systems include an increased mining footprint, potential increased loads of suspended sediments in high-rainfall catchments, changes to geo-ecological balances, and possible harmful health effects to practitioners extracting and land-applying the geological materials. Our review highlights potential for ‘geo-agriculture’ approaches to not only help meet several key emerging challenges that threaten sustainable food and fiber production, but also to contribute to achieving some of the United Nations Sustainable Development Goals—‘Zero Hunger,’ ‘Life on Land,’ and ‘Climate Action.’ Full article