Search Results (929)

Over a decade of research on media for cultured meat and seafood production has resulted in multiple highly efficient serum-free and chemically defined formulations for some species, but it has also identified challenges yet to be solved—especially for aquatic cell lines. Depending on the product and cell type, the approach to develop highly efficient, sustainable, and low-priced media can diverge greatly. In this review, we provide an in-depth overview of this complex research area to facilitate strategic decision-making for stakeholders. We evaluate the advantages and limitations of utilizing hydrolysates, growth factor mutants, growth factor alternatives, and stabilizers in serum-free media formulations published for cultured meat production, as well as ongoing research efforts on developing adequate media for cultured seafood. We critically analyze strategies aimed at reducing medium costs and enhancing sustainability of cultured meat and seafood production, including their food-compatibility assessment. We summarize topics that require further exploration, such as identification of species-specific growth factors—particularly for aquatic species; exploration of hydrolysates as a substitute for basal medium; waste medium recycling strategies; and the potential application of artificial intelligence (AI) and machine learning (ML) technologies to enhance these areas. Additionally, we consider possible emerging regulatory issues and their impact on media formulation development. Finally, key performance indicators for media formulations are proposed to guide future strategic and operational improvements regarding an economical and sustainable production process. Full article

Existing anti-aging drugs are often limited by toxicity and resistance. In contrast, natural substances derived from food resources, edible plants, and agricultural by-products offer advantages such as low toxicity and suitability for dietary intake. Utilizing these resources aligns with sustainable development goals by promoting the valorization of food waste and functional food development; however, their complex composition makes traditional discovery inefficient and resource-intensive. Machine learning (ML) provides a powerful, sustainable in silico solution. By analyzing vast datasets, computational models can rapidly screen thousands of candidates, significantly reducing the chemical waste and time associated with traditional wet-lab screening. This review focuses on the current status of food-derived anti-aging bioactives and the emerging ML-based perspectives in this field. Key natural compounds and plant extracts are discussed, highlighting their dietary origins and mechanisms. Furthermore, we explore how advanced algorithms accelerate the identification of novel bioactives. Importantly, we address current translational gaps, including the need for explainable AI, ADME (Absorption, Distribution, Metabolism, and Excretion) prediction, and the standardization of complex mixtures. Overcoming these bottlenecks is essential for the sustainable development of effective, food-based anti-aging ingredients. Full article

Insect larvae are naturally part of the diet of farmed animals, for instance poultry, pigs, and fish. Thus, the black soldier fly, Hermetia illucens (Linnaeus, 1758) (Diptera: Stratiomyidae) has been grown for use as a source of protein in animal feed. Black soldier fly larvae (BSFL) feed on various organic materials and bioaccumulate the nutrients obtained from the organic materials. This results in BSFL with protein content ranging from 25 to 60% depending on the type of organic material fed. Feeding strategies customized for optimizing BSFL growth and protein deposition are essential for sustainably increasing the production of BSFL to meet the growing demand for their use in animal feed. Feeding strategies for sustainable BSFL production should: ensure nutrient utilization efficiency to optimize BSFL growth and protein deposition; use readily available local organic material of good nutritional quality, safe, and acceptable for use in the animal feed industry; ensure economic and environmental sustainability; and adhere to existing legislature. While substantial information on feeding BSFL is available in different data sources, the literature mainly focuses on increasing BSFL production without integrating sustainability issues, especially economic and environmental sustainability. The objective of this review was to synthesize and consolidate existing information on feeding strategies for BSFL production from different sources and point out sustainable feeding strategies, existing knowledge gaps, and aspects that require further research. The purpose of the review is to provide information on feeding practices for the sustainable production of BSFL to meet the growing demand for BSFL in animal feed. This will contribute to improved food security, environmental management, and job creation. BSFL can feed on mixed organic material food sources more efficiently, reducing the volume of the food by up to 72%, while bioaccumulating the nutrients better than when feeding on individual organic sources such as fruit or vegetable waste. Full article

While Zanthoxylum bungeanum Maxim. (Z. bungeanum) pericarps are a globally prized spice, their leaves are frequently discarded as agricultural waste. This study systematically characterizes the aromatic potential of leaf by-products compared with traditional pericarps under diverse extraction strategies, utilizing an integrated flavoromics and sensomics approach. Qualitative GC-MS-O analysis revealed that leaf-derived fractions possess superior aromatic diversity: leaf essential oil and volatile solvent extract yielded 71 and 68 odorants, respectively, significantly surpassing pericarp counterparts (65 and 43 compounds). Concurrently, HS-GC-IMS profiling confirmed that targeted extraction allows leaf-derived flavors to replicate and exceed traditional spice complexity. Specifically, the leaf solvent extract achieved aromatic parity with pericarps by effectively mirroring the core spicy–citrus profile through cuminaldehyde and limonene retention. Conversely, distilled leaf essential oil unlocked a distinctive herbal–woody sensory innovation, driven by eucalyptol and a broader variety of aldehydes and ketones. Sensomics validation, incorporating aroma recombination, omission experiments, and partial least-squares regression modeling, conclusively identified β-myrcene, limonene, caryophyllene, and humulene as core molecular markers dictating these perceptual shifts. Ultimately, this research provides a robust theoretical foundation for upcycling Z. bungeanum leaves into valuable flavoring resources, facilitating circular bio-economy practices by delivering functional equivalence and entirely novel sensory experiences for the global food industry. Full article

Recirculating organic byproducts like food waste, wastewater and manure efficiently and at scale in a circular bioeconomy will be critical to ensuring future food security, energy security, climate resilience, water security and environmental health. Ultimately, we will not be able to live within the safe operating space of our planetary boundaries if we do not stop our wasteful and inefficient habits. Our food, waste, energy and water sectors are starting to transform towards circularity, driven by a diverse range of drivers, from net zero emissions targets, to food waste policies, and to rising fertiliser prices and geopolitical risks. However, these sectors are often not transforming in a coordinated manner, risking unintended consequences like competition between end-uses, technology lock-in, the prevention of scalability, or failure to achieve key sustainability targets, causing rebound effects. For example, society’s organic waste is being earmarked for the production of bioenergy, sustainable aviation fuels, biomaterials, and biofertilisers; however, it is not clear if there will be a sufficient supply of organic waste to meet these diverse demands. Phosphorus flow analyses indicate that we will need to secure almost all of the nutrients in organic waste as fertiliser raw material to produce food. There are some existing pockets of innovation within sectors related to food waste, water and wastewater, fertilisers and agriculture, and bioenergy. However, many initiatives are being driven by short-term challenges, are not operating at scale, or are not sufficiently integrated across sectors. In this paper, we provide examples of innovations and challenges from around the world, including Italy, Australia, Sri Lanka, the UK, Japan, and Malawi. This paper identifies a pathway to navigate tensions to achieve co-existing sustainability goals, including key enablers and barriers, ranging from overcoming regulatory fragmentation to a lack of capital investments. Creating a truly viable circular economy for organic byproducts requires the integration of policies, markets, technologies and people. This means engaging diverse stakeholders, from local councils and private waste contractors, farmers, and fertiliser companies to energy retailers and wastewater utilities, NGOs, informal collectors, and environmental regulators and policy-makers. Full article

The main sources of biodegradable waste come from agriculture and municipal waste, with animal manure and food waste (FW) being the most representative respectively. Most of this waste remains still unexploited, while there is skepticism regarding the environmental footprint of various methods of their utilization. This work provides a reliable comparative environmental evaluation using life cycle assessment (LCA). In the present work, LCA applied to compare two alternative scenarios regarding the management of (a) sheep and goat manure and (b) FW. Alternative scenarios for sheep and goat manure include composting for fertilizer and energy production via anaerobic digestion (AD), while FW scenarios include incineration and energy production through AD. In both case studies, the AD scenario generates environmental benefits (expressed as negative damage) across all three damage categories namely resource scarcity, human health and ecosystem quality. Regarding sheep and goat manure, the most significant effect of AD is on human health (−0.016 Pt) while in the scenarios of FW the superior performance of AD is particularly evident in the ecosystem quality (−0.21 Pt). Both case studies reached the same conclusion pointing out that the use of sustainable technologies for managing agricultural and municipal waste mitigates the environmental impacts. Full article

Sustainability in higher education plays a crucial role in shaping future professionals with an eco-conscious mindset. This study focuses on developing and simulating a portable solar food dehydrator as a practical application of sustainability principles in technology education. By integrating sustainability into the curriculum, this research enhances students’ technical skills while promoting the use of renewable energy and effective food preservation methods. Furthermore, the project aligns with green campus initiatives by encouraging energy-efficient practices and reducing food waste. This study emphasizes the significance of education for sustainable development by offering learners hands-on experience in designing eco-friendly solutions, promoting innovation, and equipping them to contribute to a more sustainable future. A food dehydrator is a device that removes moisture from food to aid in its preservation, utilizing a heat source and airflow to reduce its water content. The researchers used two methods to dehydrate food: direct sunlight (sun drying) and indirect sunlight (solar drying). The study used a developmental research design. Simulations revealed that, with solar-powered electricity, the longer the drying time, the greater the reduction in the moisture content. This was evident in the eighth experiment, which was conducted on fruits and vegetables. While drying with direct sunlight, the same trends, albeit to a lesser extent, were observed in the reduction in the moisture content of the fruits and vegetables. These insights can inform future design improvements, making the products more visually appealing and distinctive, thereby enhancing their attractiveness and novelty. Full article

Waste cooking oils (WCO) are large-scale residual streams from domestic and industrial food processing. Their improper disposal poses severe environmental risks, yet their integration into the oleochemical sector offers a strategic opportunity for the green transition by substituting fossil-based feedstocks. This systematic review provides a comprehensive assessment of WCO valorization as a sustainable precursor for high-value products, specifically biopolymers, bioplasticizers, and biolubricants. The study followed the PRISMA 2020 guidelines, searching PubMed, Scopus, and MDPI databases (up to September 2025). The search strategy utilized combinations of keywords present in the title. Inclusion criteria focused on peer-reviewed chemical and biotechnological conversion pathways published in English within the last decade. Studies addressing biofuel production, patents, and review were excluded. Screening, data extraction, and qualitative risk of bias assessment, centered on experimental reproducibility and reporting transparency, were performed independently by multiple reviewers. From an initial pool of 2637 records, 87 studies met the eligibility criteria. The analysis reveals that polyhydroxyalkanoates (PHAs) represent the most extensively researched pathway, followed by WCO-derived epoxides and innovative biolubricant formulations. While several studies report high conversion yields under optimized conditions, the transition from bench-scale to industrial implementation remains hindered by the heterogeneous composition of WCO and a lack of standardized pre-treatment protocols. WCO valorization shows transformative potential for the circular economy, offering a dual benefit of waste mitigation and sustainable material synthesis. However, future research must address scalability challenges and feedstock variability. This review identifies emerging trends and provides a roadmap for the industrial adoption of WCO-based processes in the framework of clean technologies. Full article

Paracentrotus lividus (Lamarck, 1816) is a commercially valuable species in the Mediterranean Sea, and the development of suitable feeds for juvenile rearing remains an important challenge for full-cycle aquaculture. In this study, we evaluated two experimental formulated diets containing anchovy-processing by-products and carrot peels as main ingredients, using a commercial diet as control. The diets were tested for 84 days on three size classes of P. lividus under controlled recirculating aquaculture conditions. Growth, feed intake, somatic growth rate, feed conversion ratio, gross apparent digestibility, and nitrogen assimilation were assessed. Our results demonstrated that both experimental diets could sustain the growth of sea urchins. However, growth varied significantly across diets and size classes and the two diets showed different results in terms of somatic growth rate and feed conversion ratio. Our findings suggest that eco-friendly feeds derived from industrial waste by-products can be used for sea urchin aquaculture. However, further research is needed to optimize feeding protocols and understand the underlying mechanisms affecting growth performance and feed assimilation efficiency. The utilization of food waste in aquaculture can contribute to the conservation of overexploited sea urchin stocks and promote sustainable practices in marine resource management. Full article

The increasing prevalence of Alzheimer’s disease has created a substantial and urgent need for brain-healthy functional foods. The processing of Pacific white shrimp (Litopenaeus vannamei) generates considerable amounts of head waste, which is rich in bioactive compounds, including lipids and peptides, holding great promise for the development of nutraceuticals to support human brain health. However, traditional extraction methods are time-consuming and inefficient in fully utilizing these compounds. This study aimed to explore the functional properties of these shrimp head-derived ingredients using “one-step” three-phase partitioning (TPP) followed by successive proteolysis. The extracted polar lipid (PL-SH), protein (P-SH) and proteolytic peptidic product (Pep-SH) from shrimp heads were screened for their antioxidant, neuroprotective, and anti-neuroinflammatory activities. Antioxidant activities were evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS⁺), and hydroxyl free radical scavenging assays, all of which revealed strong antioxidant potential for all three products. Neuroprotective activities were assessed using HT-22 mouse hippocampal neuronal cells challenged with Aβ₂₅₋₃₅, and anti-neuroinflammatory activities were evaluated using BV-2 microglial cells stimulated with lipopolysaccharide (LPS). The results suggested that both PL-SH and Pep-SH exerted protective effects against Aβ₂₅₋₃₅-induced cell damage under the tested conditions, and PL-SH also reduced nitric oxide (NO) production induced by LPS, indicating potential anti-neuroinflammatory activity. However, further studies with additional biomarkers (e.g., ROS, apoptosis markers, and cytokines) are required to confirm these effects. The lipid composition of PL-SH was further characterized by thin-layer chromatography and LC-MS/MS-based lipidomics, revealing various classes of phospholipids. Furthermore, analysis of the molecular weight distribution and sequences of peptides in Pep-SH revealed peptide sizes ranging from 70 to 1700 Da and a high degree of homology to known antioxidant and neuroprotective peptide sequences. These findings suggest that lipids and peptides from Pacific white shrimp heads possess valuable functional properties, supporting their potential use in the development of functional foods for neuroprotection and anti-neuroinflammation. Full article

This study proposes an integrated and more circular management approach, grounded in the principles of sustainable and green chemical processes, for the food waste leachates management, combining the assessment of biomethane production potential via anaerobic digestion with the evaluation of value-added compound recovery through extraction processes. The food waste leachates were characterized, while total carotenoid profile and total phenolic content were quantified using liquid–liquid extraction with mixed organic solvents. An HS-SPME coupled with GC–MS was employed to identify volatile organic compounds present in the leachates. Prior to the extraction procedure, D-limonene exhibited the highest abundance among identified volatiles. Crucially, the subsequent solvent extraction is highly likely to have effectively removed this inhibitory terpene from the liquid matrix. Extracted leachates exhibited a total carotenoid content of 0.64 mg/100 g and a total phenolic content of 127.0 μg/g, acting as preliminary indicators of significant potential for recovery and utilization in pharmaceutical and cosmetic applications. Biomethane potential tests were conducted in laboratory-scale anaerobic bioreactors using both raw food waste leachate and extracted food waste leachate. Comparable biomethane yields were obtained for both substrates, with FWL yielding 442.5 NmL/g VS_added and FWL_extr yielding 452.2 NmL/g VS_added. These results demonstrate that the liquid–liquid extraction of value-added compounds does not adversely affect biomethane production from food waste leachates enabling the recovery of valuable by-products. Full article

The present work investigates the chemical composition of black cumin (Nigella sativa L.) cake obtained as a by-product of cold-pressed oil extraction. The aim of the study is to assess its potential for further utilization and secondary applications. By applying a combination of analytical techniques, including chemical analysis, Soxhlet extraction, ICP-OES, ICP-MS, FTIR, and SEM-EDS, the material was characterized as a rich organic matrix with a significant residual fat content (approximately 20%), proteins, and essential mineral elements such as K, Ca, Fe, Cu, Zn, and P, while containing low levels of toxic elements. Since cold pressing preserves residual bioactive compounds, and considering the high content of essential elements, black cumin cake represents a promising ingredient for food supplements. In addition, its porous surface structure observed by SEM-EDS, together with the functional groups identified by FTIR analysis, suggests potential sorption properties. These findings position black cumin cake as a promising resource within the framework of sustainable agro-industrial waste valorization strategies. Full article

This study evaluated the effects of capsaicin on the growth and development and the nutritional composition of black soldier fly larvae (BSFL) under high-fat substrate conditions. The results showed that, under a high-fat background, low levels of capsaicin (0.01–0.05%) had a relatively small adverse effect on the growth of larvae, while 0.1–0.2% treatment led to a decrease in the accumulation of larval weight. The pupation age did not show a monotonous change consistent with the addition amount of capsaicin. The conversion efficiency of digested feed in the high-fat control group was lower than that in the basal diet control group. It slightly increased with 0.01–0.05% capsaicin treatment and decreased again with 0.2% treatment. The amino acid composition of the insect body is relatively stable overall, but the contents of glutamic acid, tyrosine, etc., decrease under high doses of capsaicin. The changes in fatty acid composition are more sensitive, mainly manifested as fluctuations in the ratios of C12:0, C18:1n-9, and C18:2n-6. The results indicate that black soldier flies have a certain tolerance to spicy and high-fat substrates, but when the addition of capsaicin increases, their growth and nutrient deposition will be adversely affected. Full article

This document proposes integrative principles that articulate fully established and validated approaches—such as ethnobotany, One Health, circular bioeconomy, TRL, and Access and Benefit-Sharing—into an integrative model that shifts bioprospecting from a purely extractive model centered on the pharmaceutical industry to a more human-centered, circular, and biocultural paradigm. Through a historical analysis of the generations of bioprospecting (from 0.0 to 4.0), this work theorizes that technological advancement has paradoxically contributed to epistemic injustice. The proposed 5.0 framework integrates social validation tools and introduces the Epistemic Gap Score (EGS) as an exploratory heuristic tool that allows for visualizing the divergence or cohesion of bioprospecting a plant resource and the recognition of ancestral knowledge in a more humanistic manner. Through preliminary proof-of-concept, this study demonstrates the practical utility of the EGS for prioritizing plant species for bioprospecting, ensuring that the valorization of underutilized plant resources produces a tangible “epistemic return” and thereby promotes community autonomy and multisectoral benefits across the food, energy, and health systems. By focusing on zero-waste cycles and social reciprocity, Bioprospecting 5.0 reestablishes the role of plant resources as essential components of sustainable global development and ethical scientific practice. Full article

Research on edible and biodegradable film packaging (EBFP) has increased significantly to explore sustainable alternatives to synthetic packaging and mitigate its environmental impacts. Biomaterials extracted from agricultural food waste (AFW) may be utilized for the fabrication of EBFP as an alternative packaging for meat and meat products. The focal point of this review is to explore the potential AFW biomaterials and bioactive compounds available in industry, and their utilization techniques for fabricating EBFP with ideal mechanical parameters suitable for use as a packaging material. Moreover, research studies have been summarized related to EBFP’s efficacy on meat shelf life, physicochemical, oxidative, and microbial qualities during storage experiments. EBFP fabricated with AFW biomaterials, such as proteins, carbohydrates, essential oils, and bioactive compounds, exhibits favorable film-forming capacity, mechanical properties, barrier properties, biodegradability, and synergy with meat. Latest advances in the application of AFW biomaterials and bioactive compounds based on EBFP for meat packaging are directed toward novel fabrication processes such as electrospinning, solvent casting, and combination of both to produce a hybrid film, which markedly improves the mechanical and barrier properties. Moreover, including bioactive materials from AFW enhances the antioxidant and antimicrobial properties of EBFP to combat the oxidative rancidity and bacteria, fungi, and molds in meat to prolong shelf life. Incorporation of AFW biomaterials and bioactive compounds has improved the intelligent properties of EBFP, which has been effectively used in meat packaging to detect freshness and spoilage of meat through color and pH changes. Full article