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Search Results (1,349)

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Keywords = extensive farms

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23 pages, 857 KiB  
Article
Study of the Impact of Agricultural Insurance on the Livelihood Resilience of Farmers: A Case Study of Comprehensive Natural Rubber Insurance
by Jialin Wang, Yanglin Wu, Jiyao Liu and Desheng Zhang
Agriculture 2025, 15(15), 1683; https://doi.org/10.3390/agriculture15151683 - 4 Aug 2025
Viewed by 219
Abstract
Against the backdrop of increasingly frequent extreme weather events and heightened market price volatility, investigating the relationship between agricultural insurance and farmers’ livelihood resilience is crucial for ensuring rural socioeconomic stability. This study utilizes field survey data from 1196 households across twelve county-level [...] Read more.
Against the backdrop of increasingly frequent extreme weather events and heightened market price volatility, investigating the relationship between agricultural insurance and farmers’ livelihood resilience is crucial for ensuring rural socioeconomic stability. This study utilizes field survey data from 1196 households across twelve county-level divisions (three cities and nine counties) from China’s Hainan and Yunnan provinces, specifically in natural rubber-producing regions. Using propensity score matching (PSM), we empirically examine agricultural insurance’s impact on household livelihood resilience. The results demonstrate that agricultural insurance increased the effect on farmers’ livelihood resilience by 1%. This effect is particularly pronounced among recently poverty-alleviated households and large-scale farming operations. Furthermore, the analysis highlights the mediating roles of credit availability, adoption of agricultural production technologies, and production initiative in strengthening insurance’s positive impact. Therefore, policies should be refined and expanded, combining agricultural insurance with credit support and agricultural technology extension to leverage their value and ensure the sustainable development of farm households. Full article
(This article belongs to the Section Agricultural Economics, Policies and Rural Management)
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22 pages, 1620 KiB  
Article
Economic Resilience in Intensive and Extensive Pig Farming Systems
by Lorena Giglio, Tine Rousing, Dagmara Łodyga, Carolina Reyes-Palomo, Santos Sanz-Fernández, Chiara Serena Soffiantini and Paolo Ferrari
Sustainability 2025, 17(15), 7026; https://doi.org/10.3390/su17157026 - 2 Aug 2025
Viewed by 353
Abstract
European pig farmers are challenged by increasingly stringent EU regulations to protect the environment from pollution, to meet animal welfare standards and to make pig farming more sustainable. Economic sustainability is defined as the ability to achieve higher profits by respecting social and [...] Read more.
European pig farmers are challenged by increasingly stringent EU regulations to protect the environment from pollution, to meet animal welfare standards and to make pig farming more sustainable. Economic sustainability is defined as the ability to achieve higher profits by respecting social and natural resources. This study is focused on the analysis of the economic resilience of intensive and extensive farming systems, based on data collected from 56 farms located in Denmark, Poland, Italy and Spain. Productive and economic performances of these farms are analyzed, and economic resilience is assessed through a survey including a selection of indicators, belonging to different themes: [i] resilience of resources, [ii] entrepreneurship, [iii] propensity to extensification. The qualitative data from the questionnaire allow for an exploration of how production systems relate to the three dimensions of resilience. Different levels of resilience were found and discussed for intensive and extensive farms. The findings suggest that intensive farms benefit from high standards and greater bargaining power within the supply chain. Extensive systems can achieve profitability through value-added strategies and generally display good resilience. Policies that support investment and risk reduction are essential for enhancing farm resilience and robustness, while strengthening farmer networks can improve adaptability. Full article
(This article belongs to the Special Issue Advanced Agricultural Economy: Challenges and Opportunities)
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23 pages, 694 KiB  
Article
Resilience for Just Transitions of Agroecosystems Under Climate Change: Northern Midlands and Mountains, Vietnam
by Tung Song Nguyen, Leslie Mabon, Huong Thu Thi Doan, Ha Van Le, Thu Huyen Thi Nguyen, Duan Van Vu and Dap Dinh Nguyen
World 2025, 6(3), 102; https://doi.org/10.3390/world6030102 - 30 Jul 2025
Viewed by 579
Abstract
The aim of this research is to identify policy and practice interventions that support a just transition towards resilient practices for resource-dependent communities. We focus on Thai Nguyen and Phu Tho, two provinces in the Northern Midlands and Mountains of Vietnam. The region [...] Read more.
The aim of this research is to identify policy and practice interventions that support a just transition towards resilient practices for resource-dependent communities. We focus on Thai Nguyen and Phu Tho, two provinces in the Northern Midlands and Mountains of Vietnam. The region is reliant on agriculture but is assessed as highly vulnerable to climate change. We surveyed 105 farming households. A Likert-type questionnaire asked respondents to self-assess their experiences of weather extremes and of changes they had made to their farming practices. Our results show that for both Thai Nguyen and Phu Tho, farmers see the effects of climate change on their crops. Respondents in Thai Nguyen were more likely to report technically driven adaptation and engagement with extension services. Respondents in Pho Tho were more likely to continue traditional practices. For both, use of traditional knowledge and practices was related to taking measures to adapt to climate change. Our main conclusion is that at least three actions could support a just transition to resilient livelihoods. First is incorporating natural science and traditional knowledge into decision-making for just transitions. Second is considering long-term implications of interventions that appear to support livelihoods in the short term. Third is tailoring messaging and engagement strategies to the requirements of the most vulnerable people. The main message of this study is that a just transition for resource-dependent communities will inevitably be context-specific. Even in centralized and authoritarian contexts, flexibility to adapt top-down policies to locals’ own experiences of changing climates is needed. Full article
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24 pages, 1000 KiB  
Article
Exploring Residents’ Perceptions of Offshore Wind Farms in Western Australia: A Qualitative Investigation
by Elena Turner and Michael Odei Erdiaw-Kwasie
Sustainability 2025, 17(15), 6880; https://doi.org/10.3390/su17156880 - 29 Jul 2025
Viewed by 297
Abstract
Residents’ attitudes towards offshore wind farms have been researched extensively over the past few decades. In this research, the precept that offshore wind farms influence residents’ well-being is implicit. Only a few studies have directly examined residents’ knowledge, perceived benefits, and acceptance. This [...] Read more.
Residents’ attitudes towards offshore wind farms have been researched extensively over the past few decades. In this research, the precept that offshore wind farms influence residents’ well-being is implicit. Only a few studies have directly examined residents’ knowledge, perceived benefits, and acceptance. This study attempts to go beyond attitude-based research and explicitly examines factors influencing acceptance decision-making. The data for this qualitative study was collected through face-to-face interviews at a proposed offshore wind farm site in Perth, Western Australia. Results from the study suggest that offshore wind farms are not perceived or responded to uniformly by residents. This study provides a more comprehensive understanding of the dynamics and complexities behind identifying and explaining how residents of designated communities perceive offshore wind farms in a nuanced manner. Therefore, this study proffers significant theoretical discussions and practical implications regarding developing sustainable renewable energy alternatives in cities across Australia. Full article
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18 pages, 1724 KiB  
Article
Ecological Product Value Realization in Agricultural Heritage System Sites: A Case Study of Wannian Rice Culture System in China
by Jingyi Li, Zhidong Li, Bojie Wang, Yan Mei, Youyu Luo and Qingwen Min
Sustainability 2025, 17(15), 6791; https://doi.org/10.3390/su17156791 - 25 Jul 2025
Viewed by 234
Abstract
The value realization of ecological products is an important part of rural and agricultural development. As a significant force for protecting traditional agricultural systems and promoting rural revitalization, agricultural heritage systems (AHSs) have formed diverse value realization paths of ecological products in the [...] Read more.
The value realization of ecological products is an important part of rural and agricultural development. As a significant force for protecting traditional agricultural systems and promoting rural revitalization, agricultural heritage systems (AHSs) have formed diverse value realization paths of ecological products in the process of dynamic protection and adaptive management. Through theoretical research, this article analyzed the characteristics of ecological products in AHS sites (EPAHSSs) and summarized the framework of value realization paths of EPAHSSs. Then, the Wannian Rice Culture System in China was selected as a case for conducting empirical research. The results showed that EPAHSSs exhibit obvious uniqueness in terms of climate environment, germplasm resources, farming and breeding models, and cultural heritage. The value realization paths of EPAHSSs mainly include industrial development support, such as the extension of agricultural industrial chains and the development of tourism, as well as fiscal transfer payments. The case analysis results indicated that Wannian County contains a rich variety of ecological products and developed a value realization pathway mainly based on the integration of industries and supplemented by fiscal transfer payments during the process of protection and development. However, further optimization is needed to promote the development of tourism and other paths. This study not only contributes to the sustainable development of the Wannian Rice Culture System, but the proposed framework is also applicable to other heritage systems and similar regions. Full article
(This article belongs to the Section Social Ecology and Sustainability)
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14 pages, 1632 KiB  
Article
Is the Mineral Content of Muscle Tissue (Longissimus Lumborum) in Cattle Finished During the Rainy Season in the Eastern Amazon Influenced by Different Farming Systems?
by Ana Paula Damasceno Ferreira, Jamile Andréa Rodrigues da Silva, Miguel Pedro Mourato, José António Mestre Prates, Thomaz Cyro Guimarães de Carvalho Rodrigues, André Guimarães Maciel e Silva, Andrea Viana da Cruz, Adriny dos Santos Miranda Lobato, Welligton Conceição da Silva, Elton Alex Corrêa da Silva, Antônio Marcos Quadros Cunha, Vanessa Vieira Lourenço-Costa, Éder Bruno Rebelo da Silva, Tatiane Silva Belo and José de Brito Lourenço-Júnior
Animals 2025, 15(15), 2186; https://doi.org/10.3390/ani15152186 - 25 Jul 2025
Viewed by 282
Abstract
The scientific literature currently lacks studies that evaluate the nutritional composition of the tissues of cattle raised in different systems, so that the nutritional effects can be known and used to enhance consumption and use in the diet. The aim was therefore to [...] Read more.
The scientific literature currently lacks studies that evaluate the nutritional composition of the tissues of cattle raised in different systems, so that the nutritional effects can be known and used to enhance consumption and use in the diet. The aim was therefore to assess whether the mineral content of muscle tissue (longissimus lumborum) in cattle finished during the rainy season in the Eastern Amazon is influenced by different farming systems. The treatments consisted of four systems (three pasture production systems and one feedlot system). 1. native wetland pasture in Santa Cruz do Arari (Mesoregion of Marajó); 2. native wetland pasture in Monte Alegre (Mesoregion of Baixo Amazonas); 3. cultivated dryland pasture in São Miguel do Guamá (Mesoregion of Nordeste Paraense); and 4. Confinement in Santa Izabel do Pará (Metropolitan Region of Belém). The analyses were carried out on samples of the longissimus lumborum muscle tissue of 48 male, castrated, crossbred Nelore cattle, twelve per breeding system, from commercial farms, destined for meat production, finished during the rainiest period of the year (between January and June). In systems 1 and 2, the animals were slaughtered in licensed slaughterhouses; the animals in systems 3 and 4 were slaughtered in commercial slaughterhouses. Food sampling and chemical analysis, soil sample collection and analysis, longissimus lumborum muscle tissue collection, sample preparation and digestion, and inductively coupled plasma optical emission spectrometry were evaluated. The experimental design was completely randomized in a linear model with four rearing systems and one period (rainy). The data was compared using the Statistical Analysis Systems (SAS) program. All analyses were carried out considering a significance level of 0.05. Samples of the diets offered (pasture and concentrate) were also collected. The Amazon systems influenced the macro- and micromineral content in the muscles of cattle (p < 0.05). The interaction between pasture systems vs. confinement showed differences in the minerals calcium (Ca), magnesium (Mg), phosphorus (P), copper (Cu), zinc (Zn), iron (Fe), and manganese (Mn) (p < 0.05). However, there was no difference in the values of sodium (Na), potassium (K), and sulfur (S) between the rearing systems (p > 0.05). By contrast, the cultivated pasture system vs. extensive pasture showed differences in all the elements evaluated (p < 0.05). The rearing systems of the Eastern Amazon influenced the mineral content of beef, which continues to be an excellent source of macro- and microminerals and can compose the human diet. Full article
(This article belongs to the Section Cattle)
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30 pages, 5720 KiB  
Review
Small-Scale Farming in the United States: Challenges and Pathways to Enhanced Productivity and Profitability
by Bonface O. Manono
Sustainability 2025, 17(15), 6752; https://doi.org/10.3390/su17156752 - 24 Jul 2025
Viewed by 1114
Abstract
Small-scale farms deserve attention and support because they play crucial and important roles. Apart from ensuring provision of food security, they also provide other economic, environmental, and social–cultural benefits. In the United States of America, these farms are agriculturally, culturally, and geographically different. [...] Read more.
Small-scale farms deserve attention and support because they play crucial and important roles. Apart from ensuring provision of food security, they also provide other economic, environmental, and social–cultural benefits. In the United States of America, these farms are agriculturally, culturally, and geographically different. They have varied needs that trigger an array of distinct biophysical, socioeconomic, and institutional challenges. The effects of these challenges are exacerbated by economic uncertainty, technological advancements, climate change, and other environmental concerns. To provide ideal services to the small-scale farm audience, it is necessary to understand these challenges and opportunities that can be leveraged to enhance their productivity and profitability. This article reviews the challenges faced by small-scale farming in the United States of America. It then reviews possible pathways to enhance their productivity and profitability. The review revealed that U.S. small-scale farms face several challenges. They include accessing farmland, credit and capital, lack of knowledge and skills, and technology adoption. Others are difficulties to insure, competition from corporations, and environmental uncertainties associated with climate change. The paper then reviews key pathways to enhance small-scale farmers’ capacities and resilience with a positive impact on their productivity and profitability. They are enhanced cooperative extension services, incentivization, strategic marketing, annexing technology, and government support, among others. Based on the diversity of farms and their needs, responses should be targeted towards individual needs. Since small-scale farm products have an effect on human health and dietary patterns, strategies to increase productivity should be linked to nutrition and health. Full article
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12 pages, 2171 KiB  
Article
Use of Foliar Biostimulants in Durum Wheat: Understanding Its Potential in Improving Agronomic and Quality Responses Under Mediterranean Field Conditions
by Angelo Rossini, Roberto Ruggeri and Francesco Rossini
Plants 2025, 14(15), 2276; https://doi.org/10.3390/plants14152276 - 24 Jul 2025
Viewed by 298
Abstract
Foliar application of biostimulants can be a valid option to reach the goal of sustainable intensification in agriculture, especially in extensive crops such as durum wheat. However, due to the wide range of active ingredients and their mixtures available in the market, the [...] Read more.
Foliar application of biostimulants can be a valid option to reach the goal of sustainable intensification in agriculture, especially in extensive crops such as durum wheat. However, due to the wide range of active ingredients and their mixtures available in the market, the need to select the most efficient product in a specific growing environment is of dramatic importance to achieve remarkable results in yield and grain quality. To analyze the potential of different active ingredients, a field trial was performed in two consecutive growing seasons (2023 and 2024) under Mediterranean climatic conditions. A randomized block design with three replicates was used. Durum wheat cultivar “Iride” was treated with the following five foliar biostimulants in comparison with the untreated control (T0): seaweed and plant extracts (T1); micronized vaterite (T2); culture broth of Pseudomonas protegens (T3); humic and fulvic acids (T4); organic nitrogen fertilizer (N 5%) containing glycine betaine (T5). Biostimulant treatment was applied at the end of tillering and at heading. Root length, chlorophyll content, grain yield, yield components and grain quality were measured and subjected to a one-way analysis of variance. As compared to the control, seaweed and plant extracts as well as micronized vaterite showed the best results in terms of grain yield (29% and 24% increase, respectively), root length (120% and 77% increase, respectively) and grain protein content (one percentage point increase, from approx. 12% to 13%). The results from this study can help Mediterranean farmers and researchers to develop new fertilization protocols to reach the goals of the “Farm to Fork” European strategy. Full article
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14 pages, 1170 KiB  
Article
Egg Quality and Nutritional Profile of Three Sicilian Autochthonous Chicken Breeds: Siciliana, Cornuta di Caltanissetta, and Valplatani
by Vittorio Lo Presti, Francesca Accetta, Maria Elena Furfaro, Antonino Nazareno Virga and Ambra Rita Di Rosa
Foods 2025, 14(15), 2571; https://doi.org/10.3390/foods14152571 - 22 Jul 2025
Viewed by 262
Abstract
The conservation of poultry biodiversity is a growing global priority, yet it necessarily relies on the scientific valorization of specific local breeds. This study aimed to characterize the lipid composition and cholesterol content of eggs from three native Sicilian chicken breeds (Cornuta, Valplatani, [...] Read more.
The conservation of poultry biodiversity is a growing global priority, yet it necessarily relies on the scientific valorization of specific local breeds. This study aimed to characterize the lipid composition and cholesterol content of eggs from three native Sicilian chicken breeds (Cornuta, Valplatani, and Siciliana) reared under semi-extensive conditions, in order to evaluate their nutritional potential and support biodiversity preservation strategies. A total of 170 eggs from 11 farms were analyzed. Fatty acid composition and nutritional indices (atherogenic index, thrombogenic index, n-6/n-3 ratio, HH index) were determined according to ISO and AOAC standards. Results showed that Cornuta eggs exhibited the most favorable lipid profile, with the lowest saturated fatty acid (SFA) content (38.55%), the lowest n-6/n-3 ratio (7.35), and the best values for AI (0.52), TI (1.22), and HH (2.02), compared to Valplatani and Siciliana. Conversely, the lowest cholesterol content was found in Siciliana eggs (1463.58 mg/kg), significantly lower than Cornuta (1789 mg/kg; p < 0.05). Although no commercial hybrids were included, the literature data were used for contextual comparison. These findings suggest that native breeds may produce eggs with functional nutritional properties, supporting both healthier food choices and local genetic conservation. Moreover, this study provides a replicable framework for the nutritional valorization of underutilized poultry breeds, reinforcing the role of biodiversity in sustainable food systems. Full article
(This article belongs to the Special Issue Eggs and Egg Products: Production, Processing, and Safety)
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12 pages, 793 KiB  
Article
Large Game as a Key Factor in the Maintenance of Tuberculosis in a Multi-Species Scenario in Southern Portugal: A Preliminary Statistical Study
by Maria Pureza Ferreira, Madalena Vieira-Pinto, Yolanda Vaz and Ana Carolina Abrantes
Zoonotic Dis. 2025, 5(3), 21; https://doi.org/10.3390/zoonoticdis5030021 - 22 Jul 2025
Viewed by 233
Abstract
Knowing the specific characteristics and animal tuberculosis risk factors present and applying good practices are crucial points in combating tuberculosis (TB) in a Mediterranean multi-species scenario. The objective of this work is to statistically analyze the association between the existence of TB in [...] Read more.
Knowing the specific characteristics and animal tuberculosis risk factors present and applying good practices are crucial points in combating tuberculosis (TB) in a Mediterranean multi-species scenario. The objective of this work is to statistically analyze the association between the existence of TB in areas with a marked game–livestock interface, with various complementary factors found in 30 extensive farms in southern Portugal, such as the number of animals of each large game species present in the territory and the frequency of their sightings. Collecting this information, an inferential statistical analysis was conducted to obtain information on the association type between TB occurrence in the farms and the presence of highlighted factors. The main statistical results show an association between the presence of large game species and TB occurrence in the analyzed areas. Thus, in a multi-species scenario, large game species are a crucial component in TB maintenance, namely when stricter contact occurs. This could be one of the reasons why TB continues to circulate and why the eradication process is so difficult; the risk of zoonotic transmission is evident. It is crucial to apply biosecurity tools to improve the alignment and structure of natural resource management strategies. Full article
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14 pages, 360 KiB  
Article
Efficiency Analysis of Sheep Farms in Cyprus
by Sokratis Sokratous, Athanasios Ragkos, Georgios Arsenos and Alexandros Theodoridis
Agriculture 2025, 15(14), 1555; https://doi.org/10.3390/agriculture15141555 - 20 Jul 2025
Viewed by 454
Abstract
In this study, an empirical analysis was applied to measure the efficiency level of dairy farms in Cyprus and estimate the capacity of sheep farmers to support the increasing demand for halloumi cheese. Data Envelopment Analysis was used on data from 50 dairy [...] Read more.
In this study, an empirical analysis was applied to measure the efficiency level of dairy farms in Cyprus and estimate the capacity of sheep farmers to support the increasing demand for halloumi cheese. Data Envelopment Analysis was used on data from 50 dairy sheep farms in Cyprus, which operate under extensive, semi-intensive, and intensive systems. The main features of the most efficient farms are presented, and a comparative financial analysis is implemented between the efficient and less efficient farms. The results indicate room for improvement in extensive and semi-intensive dairy sheep farming and verify that the transition that takes place in sheep farming towards more intensive systems constitutes the optimal approach. The most efficient farms operate under semi-intensive and intensive dairy sheep farming and achieve higher milk yields than the farms operating under extensive systems. Feeding constitutes the main cost driver, exceeding 60% in both efficient and inefficient farms, while labor wages and fixed capital cost varies between 25% and 30% of the total production cost for both efficiency groups. The findings indicate that the farms should utilize economies of scale to reduce production costs and utilize fixed capital endowments at full capacity. Full article
(This article belongs to the Special Issue Productivity and Efficiency of Agricultural and Livestock Systems)
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12 pages, 1625 KiB  
Article
Rift Valley Fever Outbreak Investigation Associated with a Dairy Farm Abortion Storm, Mbarara District, Western Uganda, 2023
by Luke Nyakarahuka, Shannon Whitmer, Sophia Mulei, Joanita Mutesi, Jimmy Baluku, Jackson Kyondo, Amy Whitesell, Carson Telford, Alex Tumusiime, Calvin Richie Torach, Dianah Namanya, Mariam Nambuya, Dominic Muhereza, Zainah Kabami, Annet Nankya, David Muwanguzi, Francis Mugabi, Nelson Wandera, Rose Muhindo, Joel M. Montgomery, Julius J. Lutwama, Stephen Karabyo Balinandi, John D. Klena and Trevor R. Shoemakeradd Show full author list remove Hide full author list
Viruses 2025, 17(7), 1015; https://doi.org/10.3390/v17071015 - 19 Jul 2025
Viewed by 506
Abstract
In Africa, Rift Valley Fever poses a substantial risk to animal health, and human cases occur after contact with infected animals or their tissues. RVF has re-emerged in Uganda after nearly five decades, with multiple outbreaks recorded since 2016. We investigated a unique [...] Read more.
In Africa, Rift Valley Fever poses a substantial risk to animal health, and human cases occur after contact with infected animals or their tissues. RVF has re-emerged in Uganda after nearly five decades, with multiple outbreaks recorded since 2016. We investigated a unique RVF outbreak associated with an animal abortion storm of 30 events and human cases on a dairy farm in Mbarara District, Western Uganda, in February 2023. Genomic analysis was performed, comparing animal and human RVF viruses (RVFV) circulating in the region. A cluster of thirteen human RVF cases and nine PCR-positive animals could directly be linked with the abortion storm. Overall, during the year 2023, we confirmed 61 human RVFV cases across Uganda, 88.5% of which were reported to have had direct contact with livestock, and a high case fatality rate of 31%. We recommend implementing extensive health education programs in affected communities and using sustainable mosquito control strategies to limit transmission in livestock, coupled with initiating animal vaccination trials in Uganda. Full article
(This article belongs to the Special Issue Emerging Highlights in the Study of Rift Valley Fever Virus)
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19 pages, 2160 KiB  
Article
Genetic Diversity and Phylogenetic Analysis Among Multidrug-Resistant Pseudomonas spp. Isolated from Solid Waste Dump Sites and Dairy Farms
by Tuhina Das, Arkaprava Das, Neha Das, Rittika Mukherjee, Mousumi Saha, Dipanwita Das and Agniswar Sarkar
Acta Microbiol. Hell. 2025, 70(3), 30; https://doi.org/10.3390/amh70030030 - 16 Jul 2025
Viewed by 375
Abstract
The excessive use of antimicrobials drives the emergence of multidrug resistance (MDR) in bacterial strains, which harbor resistance genes to survive under diverse drug pressures. Such resistance can result in life-threatening infections. The predominance of MDR Pseudomonas spp. poses significant challenges to public [...] Read more.
The excessive use of antimicrobials drives the emergence of multidrug resistance (MDR) in bacterial strains, which harbor resistance genes to survive under diverse drug pressures. Such resistance can result in life-threatening infections. The predominance of MDR Pseudomonas spp. poses significant challenges to public health and environmental sustainability, particularly in ecosystems affected by human activities. Characterizing MDR Pseudomonas spp. is crucial for developing effective diagnostic tools and biosecurity protocols, with broader implications for managing other pathogenic bacteria. Strains were diagnosed through 16S rRNA PCR and sequencing, complemented by phylogenetic analysis to evaluate local and global evolutionary connections. Antibiotic susceptibility tests revealed extensive resistance across multiple classes, with MIC values surpassing clinical breakpoints. This study examined the genetic diversity, resistance potential, and phylogenetic relationships among Pseudomonas aeruginosa strain DG2 and Pseudomonas fluorescens strain FM3, which were isolated from solid waste dump sites (n = 30) and dairy farms (n = 22) in West Bengal, India. Phylogenetic analysis reveals distinct clusters that highlight significant geographic linkages and genetic variability among the strains. Significant biofilm production under antibiotic exposure markedly increased resistance levels. RAPD-PCR profiling revealed substantial genetic diversity among the isolates, indicating variations in their genetic makeup. In contrast, SDS-PAGE analysis provided insights into the protein expression patterns that are activated by stress, which are closely linked to MDR. This dual approach offers a clearer perspective on their adaptive responses to environmental stressors. This study underscores the need for vigilant monitoring of MDR Pseudomonas spp. in anthropogenically impacted environments to mitigate risks to human and animal health. Surveillance strategies combining phenotypic and molecular approaches are essential to assess the risks posed by resilient pathogens. Solid waste and dairy farm ecosystems emerge as critical reservoirs for the evolution and dissemination of MDR Pseudomonas spp. Full article
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17 pages, 2482 KiB  
Article
Assessment of Milk Quality in Skopelos Goats Under Low- and High-Input Farming Systems
by Zoitsa Basdagianni, Ioannis-Emmanouil Stavropoulos, Georgios Manessis, Georgios Arsenos and Ioannis Bossis
Appl. Sci. 2025, 15(14), 7906; https://doi.org/10.3390/app15147906 - 15 Jul 2025
Viewed by 376
Abstract
This study investigated the effect of different farming systems and lactation stages on the physicochemical characteristics, somatic cell count (SCC), and total bacterial count (TBC) of milk from Skopelos goats. This study was conducted over two consecutive lactation periods on two commercial farms [...] Read more.
This study investigated the effect of different farming systems and lactation stages on the physicochemical characteristics, somatic cell count (SCC), and total bacterial count (TBC) of milk from Skopelos goats. This study was conducted over two consecutive lactation periods on two commercial farms in Greece, an extensive system on Skopelos Island and an intensive system in the Attica region, involving 237 goats of shared genetic background, thereby minimizing genetic variability and strengthening the validity of the comparisons between the production systems. Higher milk yields were observed in the extensive system (0.98 vs. 0.85 kg/day), while milk from this system also had a higher protein (3.57% vs. 3.47%; p < 0.001) and casein content (2.72% vs. 2.57%; p < 0.001), which are traits favorable for cheese production. Fat content peaked during mid-lactation (4.83%; p < 0.05) and remained unaffected by the farming system. Lactose declined from early (4.74%) to late lactation (4.42%; p < 0.001). Both SCC and TBC were significantly elevated in the extensive system (p < 0.001), possibly due to hand milking, environmental exposure, and less-controlled hygiene conditions. These findings highlight a trade-off between the nutritional advantages of extensive systems and challenges related to milk hygiene. A balanced approach, optimizing both quality and sustainability, is recommended. Full article
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16 pages, 1356 KiB  
Article
Impact of Light Spectrum on Tadpole Physiology and Gut Microbiota in the Dybowski’s Frog (Rana dybowskii)
by Haoyu Ji, Baolong Shan, Nan Hu, Mingchao Zhang and Yingdong Li
Animals 2025, 15(14), 2066; https://doi.org/10.3390/ani15142066 - 13 Jul 2025
Viewed by 446
Abstract
Rana dybowskii, widely distributed and extensively farmed in northeast China, holds significant economic value, particularly for its fallopian tubes, which are used as a traditional Chinese medicinal tonic known as “Oviductus Ranae.” As the light spectrum is a cost-effective regulatory factor in [...] Read more.
Rana dybowskii, widely distributed and extensively farmed in northeast China, holds significant economic value, particularly for its fallopian tubes, which are used as a traditional Chinese medicinal tonic known as “Oviductus Ranae.” As the light spectrum is a cost-effective regulatory factor in aquaculture, understanding its effects on the tadpole stage of R. dybowskii is critical for optimizing cultivation practices. This study investigated the effects of five light colors (white, red, yellow, blue, and green) on steroid hormone levels and gut microbiota composition in R. dybowskii tadpoles. Steroid hormone levels were measured on days 15, 30, 45, and 60 using high-performance liquid chromatography (HPLC), while gut microbial communities were analyzed through high-throughput 16S rRNA sequencing. Results showed that the testosterone (T) level of frogs in green light (group G) peaked on day 60 (2.62 ± 3.70 ng/g). The estradiol (E2) level in blue light (group B) also peaked on day 60 (2.87 ± 0.71 ng/g). Importantly, sex ratio analysis revealed that the proportion of females was highest under blue light, reaching 61.11%. Meanwhile, the richness and diversity of the gut bacterial community of the tadpoles was highest under yellow light, followed by blue light. These data suggest that hormone levels fluctuated and the composition of the gut flora of R. dybowskii changed under different light colors. Our results advance R. dybowskii physiological knowledge and support aquaculture practices. Full article
(This article belongs to the Section Herpetology)
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