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23 pages, 37037 KB  
Article
The Benthic Ecosystem of Mountain Top Bank, a New Mesophotic Coral Reef in the Northern Gulf of Mexico
by Bethany Pertain, Agno Rubim de Assis, Marco D’Emidio and Leonardo Macelloni
J. Mar. Sci. Eng. 2026, 14(13), 1160; https://doi.org/10.3390/jmse14131160 (registering DOI) - 23 Jun 2026
Abstract
The Gulf of Mexico, a geologically complex environment, supports mesophotic coral ecosystems, with reefs such as the Pinnacle Trend, Flower Garden Banks National Marine Sanctuary, the Florida Middle Ground reef system, and Pulley Ridge. Mountain Top Bank is a dome-shaped hardground feature located [...] Read more.
The Gulf of Mexico, a geologically complex environment, supports mesophotic coral ecosystems, with reefs such as the Pinnacle Trend, Flower Garden Banks National Marine Sanctuary, the Florida Middle Ground reef system, and Pulley Ridge. Mountain Top Bank is a dome-shaped hardground feature located 60–150 m below the sea surface along the Mississippi–Alabama shelf. It appears to prolong the Pinnacle Trend towards the southeast, bridging the gap between mesophotic coral reefs east and west of the Mississippi Canyon. Shipborne high-resolution multibeam data (bathymetry, backscatter, and water-column) and an AUV photomosaic were collected over the site during several oceanographic expeditions. Data were analyzed and compiled into an ArcGIS geodatabase to produce the first benthic habitat map of Mountain Top Bank. The site is characterized by a network of outcrops and boulders interspersed within a predominately sandy environment. Different seabed features were correlated with the presence and abundance of a diverse array of biota across the phyla of Cnidaria, Porifera, Mollusca, Chordata, Echinodermata, and Rhodophyta. We found the benthic assemblage to be similar to those found at the Pinnacle Trend, supporting the hypothesis that Mountain Top Bank is part of the same reef system and acts as a topographic bridge between ecosystems on the east and west of the Mississippi Canyon. Full article
(This article belongs to the Section Marine Ecology)
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12 pages, 585 KB  
Article
Risk of Menstrual Dysfunction, Low Energy Availability, Eating Disorders and Injury in the First All-Female UK Military Team Rowing 3000 Miles Across the Atlantic
by Solène Chaléat, David Baud, Helton De Sa Souza, Imogen O’Brien, Rebecca Glover, George Morris, Kelly Kaulback and Volker Scheer
Sports 2026, 14(6), 256; https://doi.org/10.3390/sports14060256 (registering DOI) - 22 Jun 2026
Abstract
Data on menstrual health, energy availability, and injury risk in women undertaking extreme ultra-endurance expeditions remain limited. We conducted a prospective cohort study of the first all-female UK military team competing in a 3000-mile transatlantic rowing race, aiming to characterize menstrual function, low [...] Read more.
Data on menstrual health, energy availability, and injury risk in women undertaking extreme ultra-endurance expeditions remain limited. We conducted a prospective cohort study of the first all-female UK military team competing in a 3000-mile transatlantic rowing race, aiming to characterize menstrual function, low energy availability (LEA) risk, eating disorder (ED) risk, and injury profiles. Four female British Army personnel completed the 46-day race. Menstrual symptoms, injuries, and illnesses were recorded daily, while reproductive, inflammatory, biochemical, and hematological markers were assessed before and after the race. LEA and ED risk were evaluated using the Low Energy Availability in Females Questionnaire and Brief Eating Disorder in Athletes Questionnaire, respectively. Analyses were primarily descriptive. Three athletes experienced amenorrhea during the expedition, including one with previously regular cycles. The fourth reported intermittent abnormal bleeding associated with injury and illness and screened positive for LEA risk before and after the race. Another athlete screened positive for ED risk at both time points. Most biomarkers remained stable post-race, whereas reproductive hormones showed consistent reductions in follicle-stimulating hormone and luteinizing hormone in all four participants, alongside increased oestradiol. These findings, based on a sample of four athletes, suggest that menstrual function may be sensitive to sustained physiological stress in extreme ultra-endurance settings, and support prospective monitoring in female ultra-endurance, military, and expeditionary populations. Full article
(This article belongs to the Special Issue Women's Special Issue Series: Sports)
15 pages, 20083 KB  
Article
An Environmentally Tolerant 5A Hydrogel with Photothermal Effect for Frostbite Treatment
by Jianmei Chen, Yifan Wu, Tiantian Zhu, Hongyu Wu, Meiling Su and Zongguang Liu
Gels 2026, 12(6), 554; https://doi.org/10.3390/gels12060554 (registering DOI) - 20 Jun 2026
Viewed by 138
Abstract
Rapid rewarming is the most conventional and primary treatment for frostbite, yet effective adjunctive strategies remain absent. Conventional wound dressings, such as therapeutic hydrogels, tend to freeze and lack the necessary rewarming ability, rendering them unsuitable for direct application. Herein, we engineered an [...] Read more.
Rapid rewarming is the most conventional and primary treatment for frostbite, yet effective adjunctive strategies remain absent. Conventional wound dressings, such as therapeutic hydrogels, tend to freeze and lack the necessary rewarming ability, rendering them unsuitable for direct application. Herein, we engineered an environmentally tolerant photothermal hydrogel, named 5A-Gel, featuring anti-swelling, anti-pressure, antioxidant, anti-freezing, and anti-drying capacities, for the treatment of frostbite. 5A-Gel was formed via dynamic crosslinking between gelatin and tea polyphenols in a glycerol/water solvent system. The incorporation of glycerol endowed the hydrogel with superior anti-swelling, anti-freezing, and anti-drying performance (remaining flexible at −20 °C and 37 °C for at least 60 days), along with concentration-dependent antioxidant activity due to tea polyphenols. Furthermore, 5A-Gel exhibited excellent photothermal effects, maintaining stable temperature and softness under 808 nm laser irradiation with robust cyclic durability. In addition, 5A-Gel showed slow degradability, excellent hemocompatibility, and favorable in vivo biosafety. Functionally, in a mouse frostbite wound model, photothermal rewarming therapy using 5A-Gel markedly expedited frostbite healing, promoting re-epithelialization, enhancing collagen deposition, alleviating inflammatory response, and stimulating neovascularization. Therefore, the as-prepared 5A-Gel serves as a competent therapeutic platform for in situ frostbite treatment and offers innovative principles for the rational engineering of high-performance hydrogel systems targeting frostbite tissue injuries. Full article
(This article belongs to the Special Issue Novel Hydrogels for Drug Delivery and Regenerative Medicine)
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28 pages, 19571 KB  
Article
Underway Shadowgraphic Imaging for Plankton Detection and Classification
by Rubens M. Lopes, Leandro T. De-La-Cruz, Luis F. Baldasso, Josiane Lima, Stelamari Y. Ito, Gelaysi Moreno and Paulo S. Polito
J. Mar. Sci. Eng. 2026, 14(12), 1129; https://doi.org/10.3390/jmse14121129 (registering DOI) - 19 Jun 2026
Viewed by 180
Abstract
Technological advances in hardware and software have enabled the development of novel in situ plankton imaging systems to investigate the spatial and temporal distribution of plankton communities. State-of-the-art machine learning approaches have been applied for automated image classification, effectively handling the complex and [...] Read more.
Technological advances in hardware and software have enabled the development of novel in situ plankton imaging systems to investigate the spatial and temporal distribution of plankton communities. State-of-the-art machine learning approaches have been applied for automated image classification, effectively handling the complex and highly variable morphology of plankton while maintaining high accuracy. Despite these advances, few instruments can acquire zooplankton images autonomously in a continuous underway mode, which is essential for large-scale oceanographic surveys conducted aboard research vessels or ships of opportunity. Here, we present SiMFlux, an underway shadowgraphic imaging system developed at the University of São Paulo, and report results from the Orient Expedition. Observations were conducted aboard an 80-foot sailing vessel navigating across the Indian and Atlantic Oceans. A total of 193 videos were analyzed from daily route segments, yielding over 1.2 million regions of interest (ROIs) containing organisms and detrital particles. Particles were automatically classified and subsequently validated by plankton experts. Full article
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5 pages, 176 KB  
Editorial
Ecotourism and the Hidden Ecology of Infection: The Andes Virus Cruise Outbreak
by Laura Scorzolini, Alessandra D’Abramo, Enrico Girardi and Emanuele Nicastri
Infect. Dis. Rep. 2026, 18(3), 59; https://doi.org/10.3390/idr18030059 - 16 Jun 2026
Viewed by 136
Abstract
Over the past decade, outdoor recreational activities—including ecotourism, wildlife observation, adventure travel, and expedition cruising—have expanded at an unprecedented pace [...] Full article
(This article belongs to the Section Viral Infections)
22 pages, 7796 KB  
Article
Sensorless Speed Control of PMSMs Based on an Improved Fast Power Reaching Law
by En Lu, Yufei Liu, Minghui Zhang and Jinyong Ju
Sensors 2026, 26(12), 3737; https://doi.org/10.3390/s26123737 - 11 Jun 2026
Viewed by 282
Abstract
Traditional permanent magnet synchronous motor (PMSM) control systems rely on mechanical position sensors for high-precision rotor position and speed information, which increases hardware complexity, raises system cost, reduces reliability, and limits adaptability to harsh environments. To overcome the above limitations, this paper proposes [...] Read more.
Traditional permanent magnet synchronous motor (PMSM) control systems rely on mechanical position sensors for high-precision rotor position and speed information, which increases hardware complexity, raises system cost, reduces reliability, and limits adaptability to harsh environments. To overcome the above limitations, this paper proposes a novel high-performance sensorless speed control strategy for PMSMs, which is constructed based on a non-singular terminal sliding mode observer (NTSMO) and a non-singular terminal sliding mode controller (NTSMC). First, an improved fast power reaching law (IFPRL) is proposed, which consists of a variable exponential reaching term and a power reaching term. Specifically, the gain of the exponential reaching term is dynamically adjusted by the absolute value of the sliding mode switching function, enabling the reaching law to operate in two different modes throughout the entire convergence process of the system state. Moreover, the introduction of scaling coefficient c compensates for the performance degradation caused by variations in the range of sliding mode surfaces (SMSs) in different systems. The proposed IFPRL not only effectively mitigates the inherent chattering issue, it also expedites the rate at which the system state converges to its SMS. On this basis, both the NTSMO for rotor position observation and the NTSMC for speed closed-loop control are designed by embedding the proposed IFPRL into the framework of non-singular terminal sliding mode control theory. Finally, the effectiveness of the proposed method is validated through numerical simulations and experimental tests. Experimental results demonstrate that the proposed IFPRL-based NTSMC + NTSMO scheme reduces the root mean square error (RMSE) of speed control by 2.7% relative to the traditional SMC + SMO method. The proposed method realizes reliable sensorless speed control for PMSMs and exhibits superior dynamic response, higher control accuracy, and stronger robustness against disturbances. Full article
(This article belongs to the Special Issue Novel Sensing Methods in Advanced Manufacturing Systems)
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49 pages, 4724 KB  
Article
A Modified Complex-Valued Encoding Greater Cane Rat Algorithm for Global Optimization and Constrained Engineering Applications
by Yubao Xu, Yuebo Wu and Jinzhong Zhang
Biomimetics 2026, 11(6), 413; https://doi.org/10.3390/biomimetics11060413 - 11 Jun 2026
Viewed by 307
Abstract
The greater cane rat algorithm (GCRA) draws inspiration from the seasonal behavioral patterns of the greater cane rats: extensive roaming during the non-breeding period for global exploration, and aggregative foraging during the reproductive period for local exploitation. The GCRA leverages independent movement and [...] Read more.
The greater cane rat algorithm (GCRA) draws inspiration from the seasonal behavioral patterns of the greater cane rats: extensive roaming during the non-breeding period for global exploration, and aggregative foraging during the reproductive period for local exploitation. The GCRA leverages independent movement and population aggregation to iteratively update positions in pursuit of the optimal solution, which exhibits inherent structural deficiencies: precipitous population diversity collapse, lethargic convergence dynamics, suboptimal computational precision, high susceptibility to local optima, and severe dimensional scalability. This paper proposes a modified complex-valued encoding GCRA (CGCRA) that exploits the mathematical structure of complex numbers to construct a two-dimensional search domain on the complex plane and facilitate collaborative optimization. The CGCRA maps the decision variables onto the complex domain, the real part executes the native foraging mechanism for local fine-grained exploitation, and the imaginary part exploits phase rotation to generate global exploratory perturbations. The CGCRA leverages a dual-encoding redundancy mechanism with inherent error tolerance to attenuate result volatility, augment information capacity and population heterogeneity, elevate search adaptability and disturbance rejection, accelerate parallel computation and exploration efficiency, and facilitate spatial transformation and multi-dimensional data manipulation. Twenty-three benchmark functions and twelve real-world engineering designs are employed to assess the CGCRA’s stability and practical feasibility rigorously. The CGCRA delivers comprehensive spatial mapping and adaptive coordination to facilitate population collaboration and bolster resilience, expedite exhaustive research, and advance optimization efficiency. The experimental results demonstrate that the CGCRA emphasizes instructive superiority and practical utility to regulate exploration and exploitation, reduce result dispersion, mitigate search stagnation, accelerate convergence efficiency, elevate solution precision, and fortify stability and robustness. Full article
(This article belongs to the Section Biological Optimisation and Management)
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39 pages, 8016 KB  
Article
An Implementation Strategy for VOC Emissions Eco-Labeling in Wood Products for the Chilean Market with International Projection
by Gabriel Cereceda-Balic, Marcela Vidal-Vega, Mario Núñez-Decap and Cecilia Fuentealba-Becerra
Sustainability 2026, 18(12), 5901; https://doi.org/10.3390/su18125901 - 9 Jun 2026
Viewed by 164
Abstract
This study examines the global wood products market to propose an eco-label for the Chilean sector with an international orientation. A national prior certification framework and governance structure were established, aligned with international regulations to ensure compliance with target market standards. By doing [...] Read more.
This study examines the global wood products market to propose an eco-label for the Chilean sector with an international orientation. A national prior certification framework and governance structure were established, aligned with international regulations to ensure compliance with target market standards. By doing so, the initiative aims to expedite the entry of Chilean products into foreign markets while incentivizing the supply and demand for sustainable goods locally. Through a historical review of eco-labels and an assessment of environmental declarations in the timber industry, this research identified critical regulations and designed a comprehensive management model. The findings reveal that existing certifications predominantly focus on indoor air quality (IAQ), specifically regarding volatile organic compound (VOC) and formaldehyde (HCHO) emissions in wood products for the construction sector (CLT, GLULAM, panels, etc.) using fossil-based adhesives. This study concluded that governance should be spearheaded by the Chilean Environment Ministry in collaboration with a specialized technical committee. Consequently, as a result, a hybrid (quantitative and qualitative) eco-label is proposed to communicate emissions limit compliance, enabling Chilean firms to report it emissions, prior to initiating formal international certification processes. Full article
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21 pages, 2296 KB  
Article
Formulation, Physicochemical Optimization, and Forensic Evaluation of Zinc Oxide- and Curcumin-Loaded Solid Lipid Nanoparticles for Safe Fingerprint Detection in Forensic Medicine
by Ahmed A. Katamesh, Rehab Abdelmonem, Sarah A. Khater, Hadel A. Abo El-Enin, Abdullah A. Alshehri, Noran Khaled, Khadiga A. Fattah and Inas Essam Ibrahim Al-Samadi
Pharmaceuticals 2026, 19(6), 904; https://doi.org/10.3390/ph19060904 - 6 Jun 2026
Viewed by 385
Abstract
Purpose: Nano-forensics is the latest application of nano-based technology for the purpose of fingerprint detection to improve precision, expedite investigations, and enhance safety. Solid lipid nanoparticles (SLNs) represent a promising pharmaceutical nanocarrier system for different applications. This study focused on applying ZnO [...] Read more.
Purpose: Nano-forensics is the latest application of nano-based technology for the purpose of fingerprint detection to improve precision, expedite investigations, and enhance safety. Solid lipid nanoparticles (SLNs) represent a promising pharmaceutical nanocarrier system for different applications. This study focused on applying ZnO and/or curcumin nanoparticles (NPs) to SLNs for the purpose of fingerprint detection to improve their sensitivity, safety and selectivity. Methods: A factorial design was utilized to select the optimized Cur-SLNs and ZnO-SLNs on the basis of the smallest particle size (PS), the lowest polydispersity index (PDI) and the highest zeta potential (ZP) value. To select the safe SLN-NPs, a cytotoxicity test was applied and they were compared to the most commonly applied product in fingerprint detection. The optimized formula was investigated according to the morphological structure; confocal spectroscopy and a stability study at different storage conditions were applied. Then the SLN-NPs were evaluated for their sensitivity, efficacy and selectivity in fingerprint detection. Results: The obtained optimal Cur-SLNs and ZnO-SLNs showed a nano PS of 221.55 ± 1.34 nm and 313.950 ± 1.87 nm, respectively, a PDI value < 0.7 and a ZP > 20 mV. The cytotoxicity data demonstrate that Cur-SLNs have low toxicity, so they will be the chosen formula. TEM and Raman spectroscopy analysis of the optimized Cur-SLN formulation validated the encapsulation efficiency and structural integrity of the pharmaceutical nanosystem. Furthermore, the powder showed stability and good results with higher adherence but smudged the prints on surfaces due to the slightest moisture. Conclusions: Overall, the results confirmed that Cur-SLN nanopowders can be developed as a suggested alternative to the current toxic powders used for latent fingerprint detection in forensic science, but only after further research on various surfaces and in different conditions. Full article
(This article belongs to the Special Issue Pharmaceutical Formulation Characterization Design, 2nd Edition)
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18 pages, 2044 KB  
Article
Density and Abundance of Green Turtles in the Saudi Arabian Red Sea
by Nicolas J. Pilcher, Cambria Davies, Eleanor Bowen, Sultan Abdullah Alturki, Tariq Alqahtani, Khalid Imam, Modar Al Sulaimani, Collin T. Williams, Carlos M. Duarte and Mohammed Ali Qurban
Ecologies 2026, 7(2), 50; https://doi.org/10.3390/ecologies7020050 - 5 Jun 2026
Viewed by 338
Abstract
Effective management and conservation of sea turtles is often constrained by a lack of knowledge of at-sea distribution and abundance. While abundance estimates of nesting females are typically well-documented on nesting beaches, counting sea turtles at sea presents challenges due to their widespread [...] Read more.
Effective management and conservation of sea turtles is often constrained by a lack of knowledge of at-sea distribution and abundance. While abundance estimates of nesting females are typically well-documented on nesting beaches, counting sea turtles at sea presents challenges due to their widespread distribution and cryptic habits. Given nesting beaches only document adult females, at-sea data are also more informative of greater population demographics. To estimate the abundance and density of green sea turtles (Chelonia mydas) in the Red Sea waters of Saudi Arabia we conducted strip transect aerial surveys in four survey zones that spanned ~66% of shallow water habitats (<200 m depth), within which we counted sea turtles, and also other species such as dugongs and other marine mammals, sharks, and rays. Corresponding abundance estimates were modelled to account for perception bias (whether a surveyor saw a turtle that was available) and detection bias (whether a turtle was available to be seen). Our results suggest an abundance of ~201,427 green sea turtles potentially present between the 200 m bathymetric contour and the Saudi Arabian shore. However, there was a statistically significant relationship between turtle location and proximity to coral reefs, with over 90% of turtles found within 3500 m of coral reef structures (whether coastal fringing reefs, barrier reefs or atolls), and therefore it would be inappropriate to use an estimate assuming equal distribution. Adjusting for this buffer area we estimated ~95,000 turtles (95% CI: 64,000–142,000) within the proximity of reef structures. These findings represent the first abundance estimates of green turtles in the Red Sea. Repeated over time, surveys such as these can identify changes in population structure, distribution and abundance, and inform conservation and management agencies. Full article
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15 pages, 2805 KB  
Review
Anatomy and Pathology of Anterior and Lateral Hip Compartments Bursae: An Ultrasound Structured Approach in Sports Medicine
by Antonio Corvino, Corrado Tagliati, Vincenzo Ricci, Fabio Corvino, Domenico Tafuri, Orlando Catalano and Giulio Cocco
Diagnostics 2026, 16(11), 1731; https://doi.org/10.3390/diagnostics16111731 - 4 Jun 2026
Viewed by 712
Abstract
When assessing hip pain in athletes, it is important to focus on the extra-articular soft tissues that may clinically mimic joint pathology. One such extra-articular structure is the synovial bursa. Bursitis can clinically be misdiagnosed as joint-, tendon- or muscle-related pain. These pathological [...] Read more.
When assessing hip pain in athletes, it is important to focus on the extra-articular soft tissues that may clinically mimic joint pathology. One such extra-articular structure is the synovial bursa. Bursitis can clinically be misdiagnosed as joint-, tendon- or muscle-related pain. These pathological processes are a result of inflammation that is often secondary to acute trauma, overuse injuries, arthritis, or infection. Traumatic and overuse hip bursitis occur frequently in amateur and professional athletes and clinical presentation is often nonspecific. After clinical assessment, imaging plays an important role in diagnosis and in work-up of these lesions. Ultrasonography (US) is being increasingly used in the assessment of the hip because of the wide availability of US machines, the lower cost, and the unique real-time imaging capability which allows both static and dynamic evaluation as well as guidance of interventional procedures such as fluid aspiration and steroid injection. In order to obtain a correct diagnosis, an efficient US examination requires a thorough understanding of hip anatomy. In this setting, a structured approach based on identification of important US landmarks and compartmentalization of hip anatomy can significantly simplify the task. Sports physicians must be aware about the diagnostic and therapeutic possibilities offered by US in order to expedite rapid referral to a musculoskeletal specialist who can perform a point-of-care US examination of the hip by using a structured diagnostic approach. The purpose of this narrative review is to illustrate both the normal and pathological features of anterior and lateral hip compartments bursae using schematic diagrams and corresponding US images. We will concentrate on sport-related bursitis, of which we will discuss the etiology, clinic and principles of care management. Our aim is to promote the acquisition of these concepts in sports medicine in order to improve patient outcomes. Full article
(This article belongs to the Section Medical Imaging and Theranostics)
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30 pages, 17700 KB  
Article
Cross-Expedition Domain Adaptation for Polymetallic Nodule Detection: A Multi-Model Pseudo-Labelling Approach
by Gabriel Loureiro, André Dias and Eduardo Silva
J. Mar. Sci. Eng. 2026, 14(11), 1048; https://doi.org/10.3390/jmse14111048 - 3 Jun 2026
Viewed by 260
Abstract
The automated detection of deep-sea polymetallic nodules is critical for processing large volumes of benthic imagery. However, its scalability faces challenges from cross-expedition covariate shifts, such as changes in lighting, altitude, and camera payloads, which lower zero-shot model performance. While semi-supervised pseudo-labelling presents [...] Read more.
The automated detection of deep-sea polymetallic nodules is critical for processing large volumes of benthic imagery. However, its scalability faces challenges from cross-expedition covariate shifts, such as changes in lighting, altitude, and camera payloads, which lower zero-shot model performance. While semi-supervised pseudo-labelling presents a potential alternative to time-consuming re-annotation, simple implementations can quickly lead to confirmation bias. This study identifies two primary sources of this degradation: spatial noise from tiling fragmentation at tile borders and an architecture-agnostic interior false positive floor caused by semantic domain shift. This work proposes using a multi-model ensemble for pseudo-labelling to reduce the noise impact. Using a spatial border filter and confidence stratification, three architecturally distinct teacher models (YOLOv8, Faster R-CNN, and DINO) are employed to determine a reliable and domain-invariant subspace. Under a strict anti-leakage Leave-One-Partition-Out protocol, the proposed approach surpasses the supervised fine-tuning baseline at 100-tile pseudo-label budget across four random seeds (macro mAP50:95 of 0.4745±0.0042 versus 0.4467±0.0079), with gains concentrated in the most domain-shifted fold. Beyond this budget, our findings highlight two important adaptation trends: a pool-size degradation trend where excessive pseudo-label volume actively degrades generalisation, and the observation that the fine-tuned models reduce pseudo-label fidelity despite higher precision, providing evidence for the advantage of using frozen source checkpoints for cross-domain adaptation. Full article
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28 pages, 1721 KB  
Article
Environmental Investigations of Travel-Associated Legionnaires’ Disease Cases: Timeliness, Microbiological Findings, and Public Health Response
by Antonios Papadakis, Eleftherios Koufakis, Dimosthenis Chochlakis and Anna Psaroulaki
Microorganisms 2026, 14(6), 1253; https://doi.org/10.3390/microorganisms14061253 - 2 Jun 2026
Viewed by 539
Abstract
In Europe, travel-associated Legionnaires’ disease (TALD) cases require timely environmental investigations to support risk assessment, rapid control measures, and prompt reporting of investigation findings to the European Legionnaires’ Disease Surveillance Network (ELDSNet). This study evaluated TALD-related environmental investigations conducted during 2025 and early [...] Read more.
In Europe, travel-associated Legionnaires’ disease (TALD) cases require timely environmental investigations to support risk assessment, rapid control measures, and prompt reporting of investigation findings to the European Legionnaires’ Disease Surveillance Network (ELDSNet). This study evaluated TALD-related environmental investigations conducted during 2025 and early 2026 in Crete, Greece, following notifications through ELDSNet. Overall, 30 notifications corresponded to 24 unique confirmed TALD cases with illness onset in 2025 and 24 implicated hotels, with some cases involving stays in multiple hotels and Regional Units and clusters identified in some implicated hotels. The investigation framework combined microbiological, physicochemical, and operational data, focusing on delays from symptom onset, notification, sampling, and laboratory reporting. Overall, 516 environmental samples were collected, of which 503 yielded valid analytical results. Among the 503 samples analyzed, Legionella spp. were detected at ≥50 colony-forming units per liter (CFU/L) in 127 samples (25.25%). This included 123 samples positive for L. pneumophila (24.45%), of which 31 were serogroup 1 (6.16%). Concentrations exceeding the 1000 CFU/L threshold were recorded in 53 samples (10.54%). Operational indicators varied, with median values of 31.0 days for reporting delay (RD), 14.5 days from notification to first sampling (TTF), 47.5 days from symptom onset to first sampling (TDS), and 67.0 days from symptom onset to first laboratory result (OELR). The findings underscore the necessity to document response delays, enhance inspector capacity and cross-regional coordination, and integrate microbiological results with operational indicators. This integration is crucial for facilitating earlier environmental risk assessments, expediting reporting, and implementing more effective TALD public health interventions. Full article
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12 pages, 1979 KB  
Proceeding Paper
Evaluation of Optimization Methods for EV and REDG Integration into the Power System Under Various Operational Scenarios
by Mlungisi Ntombela and Musasa Kabeya
Eng. Proc. 2026, 140(1), 39; https://doi.org/10.3390/engproc2026140039 - 28 May 2026
Viewed by 303
Abstract
The exhaustion of fossil fuels, environmental concerns, and difficulties in deploying smart grids have expedited the development of renewable energy distributed generators (REDGs) and electric vehicles (EVs). In recent decades, there has been a notable rise in the production and marketing of EVs. [...] Read more.
The exhaustion of fossil fuels, environmental concerns, and difficulties in deploying smart grids have expedited the development of renewable energy distributed generators (REDGs) and electric vehicles (EVs). In recent decades, there has been a notable rise in the production and marketing of EVs. Previous research has proposed reactive power control solutions, including the use of power electronic converters associated with distributed generators (DGs) to alleviate voltage fluctuations. This research presents a strategy for the best integration of electric vehicles through bidirectional charging and renewable energy distributed generators inside power systems, with the objective of efficiently managing voltage, active power, and reactive power flows at interconnection points. Furthermore, it entails determining appropriate locations and dimensions for electric car charging stations through a comparative examination of computing time and iterations between the Hybrid Genetic Algorithm Improved Particle Swarm Optimization (HGAIPSO) and several other optimization methods, including Genetic Algorithm (GA), Particle Swarm Optimization (PSO), and Improved Particle Swarm Optimization (IPSO). This analysis was performed on the IEEE-118 bus system, incorporating Vehicle-to-Grid (V2G), Grid-to-Vehicle (G2V), and REDG allocations. The simulation results indicated that the suggested HGAIPSO approach is more rapid and effective regarding calculation time for complex networks, attaining optimal solutions with greater efficiency. Full article
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25 pages, 17133 KB  
Article
A Gene Feature Based on Histone Modifications Can Predict the Prognosis of Prostate Cancer
by Jialin Gao, Xuee Zhou, Zetao Zuo, Jiahong Hong, Yan Tan, Xiaoxiang Rong, Rui Zhou and Zhenhua Huang
Biomedicines 2026, 14(6), 1219; https://doi.org/10.3390/biomedicines14061219 - 28 May 2026
Viewed by 199
Abstract
Background/Objectives: Prostate cancer (PCa) remains a prevalent malignancy among men, often complicated by recurrence and unfavorable clinical outcomes. Consequently, precise risk stratification and timely clinical intervention are paramount. Initially, we delineated distinct expression profiles of histone modification regulators via unsupervised clustering, identifying [...] Read more.
Background/Objectives: Prostate cancer (PCa) remains a prevalent malignancy among men, often complicated by recurrence and unfavorable clinical outcomes. Consequently, precise risk stratification and timely clinical intervention are paramount. Initially, we delineated distinct expression profiles of histone modification regulators via unsupervised clustering, identifying PCa subtypes with divergent survival probabilities and biological phenotypes. Subsequently, we sought to develop a prognostic gene signature, derived from the transcriptomic variations among these regulator-defined subtypes, to predict outcomes in PCa patients following radical prostatectomy (RP). Methods: Clinical and transcriptomic data from PCa cohorts were retrieved from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) repositories for comprehensive analysis. Subtypes driven by histone modification regulators were established using unsupervised consensus clustering, followed by in-depth characterization of their molecular features and associated pathways. A risk-scoring model was then developed to evaluate its prognostic efficacy in this patient population. Results: Stratification based on histone modification regulators yielded four distinct PCa subtypes exhibiting heterogeneous survival outcomes, functional pathways, and genomic mutational landscapes. Following rigorous feature selection, a 21-gene risk signature (HIS_score)—comprising MXD3, CCDC28B, COL11A2, SLC39A5, GPT, DNASE1L2, PIF1, KRTAP5-9, TTLL10, KRTAP5-1, KRTAP5-10, HAGHL, MSLNL, AMH, NKAIN4, CCDC114, SLC9A3, SULT1E1, ALB, SLC6A14, and RPE65—was constructed. Survival analyses demonstrated that patients assigned to the high HIS_score cohort experienced significantly worse clinical outcomes compared to their low-score counterparts. Furthermore, we integrated this signature into a novel clinical nomogram to facilitate individualized prognostic assessments. Conclusions: Derived from transcriptomic disparities between extreme epigenetic subtypes, the HIS_score and its associated nomogram serve as robust prognostic instruments. These tools effectively encapsulate the downstream transcriptional sequelae of histone modification dysregulation, offering clinicians a valuable framework to accurately predict post-RP outcomes and expedite the formulation of personalized therapeutic strategies. Full article
(This article belongs to the Section Cancer Biology and Oncology)
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