Search Results (78)

Climate change reshapes crop–weed interactions and challenges the cultivation of oilseed rape (Brassica napus L.). Common ragweed (Ambrosia artemisiifolia L.) strongly suppresses early crop development, increases stress sensitivity and leads to yield loss. The stress–physiological responses of oilseed rape to ragweed competition were investigated using a combination of conventional and non-invasive methods. A pot experiment was conducted with increasing ragweed densities (0, 1, 3, 5 and 10 plants). Plant height and biomass were evaluated via non-destructive indicators (SPAD, NDVI) and different stages (1–15 and 16–30 min) of delayed fluorescence (DF) alongside ferric reducing antioxidant power (FRAP). Increasing ragweed density caused changes in growth, altered DF magnitude and decay kinetics, indicating photosynthetic imbalance. Moderate weed competition (1–5) induced an adaptive, eustress-like response characterised by enhanced non-enzymatic antioxidant capacity, whereas higher ragweed densities overwhelmed this compensatory mechanism, resulting in oxidative stress-like responses. Among all measured traits, DF_1–15 proved to be the earliest and most sensitive indicator of the transition from adaptive to disruptive stress: T1: 0 ragweed: 213.07 ± 10.36 cps/mm² and 92.66 ± 6.67 cps/mm². These results demonstrate that delayed fluorescence, combined with conventional physiological and antioxidant-based parameters, enables the early detection of competitive stress in oilseed rape well before visible symptoms appear. Full article

The purpose of this study is to examine how employees’ artificial intelligence awareness (AIA) influences adaptive performance in the workplace through the mediating roles of eustress and task crafting within the Job Demands–Resources (JD-R) Theory. Data were collected from 372 full-time employees working in five-star hotels and analyzed using PLS-SEM with WarpPLS. The findings reveal that employees’ AI awareness significantly enhances adaptive performance both directly and indirectly. AI awareness also positively predicts eustress and task crafting, suggesting that informed employees experience motivating stress and actively reshape their tasks to optimize work processes. Moreover, both eustress and task crafting serve as significant mediators, amplifying the effect of AI awareness on adaptive performance. These results underscore the value of cultivating AI knowledge among employees to foster proactive behaviors and positive stress responses, ultimately supporting adaptability in dynamic work environments. The study contributes to JD-R Theory by integrating AI-related awareness as a personal resource driving employee adaptation. Full article

Wood vinegar (WV), a by-product of woody biomass pyrolysis, is increasingly used in agriculture as a sustainable biostimulant, although its effects on plant stress resistance and underlying mechanisms remain poorly understood. Recent studies propose that WV may act through a eustress-based mechanism, defined as a mild and controlled stress that activates adaptive physiological responses and enhances plant performance without causing structural or metabolic damage. This study investigated the physiological and biochemical effects of WV on strawberry plants grown under three water-deficit stress levels [no stress (NS), moderate stress (MS), and high stress (HS)] and treated with WV either via fertigation (0.5% v/v, WV₁) or foliar spray (0.2% v/v, WV₂). Gas exchange parameters (A, g_sw, E, Ci, WUE), total chlorophyll content, and nutrient balance ratios (Fe/Mn and K/Ca) were measured after a three-month growth period. PERMANOVA revealed significant effects of both WV and water-deficit stress, as well as their interaction, on most parameters. Under NS and MS conditions, WV reduced A, gsw, E, and Ci while increasing WUE, indicating enhanced water-use efficiency and improved physiological adjustment to water limitation. Chlorophyll content remained stable, demonstrating preserved photosynthetic integrity. Nutrient ratios further supported a controlled ion rebalancing associated with adaptive stress responses under NS and MS, whereas HS conditions indicated the onset of distress. Overall, the data demonstrate that WV enhances plant stress resistance primarily by inducing eustress-mediated physiological regulation rather than by directly stimulating growth. Full article

In recent years, the acceleration of climate change and the growing demand for higher-quality food to meet the needs of an expanding population have become pressing challenges. Nanotechnology has emerged as a promising tool in agriculture, particularly through the application of nanoparticles (NPs). Recent studies highlight their potential to enhance plant performance, improve resistance to environmental stresses, and act as eustressors—stimuli that activate beneficial adaptive responses. Nanoparticles have been shown to stimulate plant defense systems (elicitation), promote growth and productivity, and improve crop quality by modulating physiological and biochemical pathways. Their role in enhancing adaptive capacity under diverse stress conditions makes them valuable candidates for sustainable agricultural strategies. However, a critical knowledge gap remains: the definition of eustress dose intervals. Establishing these thresholds is essential for maximizing the positive effects of NPs while minimizing risks. Finally, the need to define safe eustress dose intervals is highlighted as a critical step toward maximizing agricultural benefits while minimizing ecological and health risks. Full article

This study examines how employees’ psychological capital relates to workplace stress and mobbing (also known as workplace bullying) across three European countries. Stress has become an increasingly dominant issue globally since the second half of the 20th century, moving from clinical contexts into public awareness. It is now recognized as a significant health risk factor, particularly in work environments. While positive forms of stress (eustress) can enhance performance, chronic workplace stress is linked to serious mental and physical health problems. This study investigates the relationship between psychological capital (PsyCap), workplace stress, and mobbing among employees in Germany, Austria, and Hungary. Based on a cross-sectional survey (N = 89), the research applied validated instruments (PCQ, PSS-10, COPSOQ II) to measure PsyCap, perceived workplace stress, and experiences of mobbing. Results show a high average PsyCap level (M = 4.64, SD = 0.70) and a moderate perceived workplace stress level (M = 2.73, SD = 0.62) across the sample. A strong negative correlation was identified between PsyCap and workplace stress (r = −0.573, p < 0.001), while a moderate positive correlation was found between workplace stress and mobbing experiences (r = 0.323, p = 0.002). Although PsyCap moderated stress levels, it did not significantly moderate the relationship between mobbing and perceived stress. These findings emphasize the role of PsyCap in reducing workplace stress and underline the necessity of organizational interventions in promoting psychological resilience and mobbing prevention. The results also indicate a need to further examine the causal relationship between mobbing, stress, and PsyCap. Full article

The use of a wide variety of antioxidants has been advocated as a means to prevent, delay the progression of, or counteract the adverse consequences of sarcopenia, such as loss of muscle strength, muscle quantity/quality, and physical performance. However, these proposals do not always appear to be supported in the literature by a thorough understanding of the contribution of redox perturbations to the pathogenesis of sarcopenia, nor of the biochemical properties, mechanism of action, pharmacokinetics, and pharmacodynamics of different antioxidants. This review discusses these aspects, aiming to provide a rationale for the selection and use of antioxidants in sarcopenia. After providing a definition of sarcopenia in the context of frailty, we distinguish between oxidative eustress as a physiological response of muscle cells to mild stimulation, such as moderate exercise, mediating their capacity for adaptation and regeneration, and oxidative distress as a pathophysiological response to muscle cell damage and death. The role of oxidative damage to biological macromolecules, both direct and mediated by advanced lipid peroxidation end products and advanced glycation/glycoxidation end products, is examined in detail. Next, we discuss antioxidant defense mechanisms, both enzymatic and non-enzymatic, including redox-sensitive gene regulatory events presided over by nuclear factor erythroid 2-related factor 2, the master regulator of enzymatic antioxidants. The review then discusses criteria for a rational classification of non-enzymatic antioxidants. This is followed by a review of some of the main radical-trapping antioxidants, both phenolic and non-phenolic, whose characteristics are compared. Full article

Numerous scientific studies have confirmed the effectiveness of seed bioactivation using electromagnetic fields (EMFs) in agriculture. This article presents the results of the remote application of an EMF TOR device in the cultivation of barley Hordeum vulgare L. Laboratory studies and field tests were conducted, showing a positive effect on the growth and development of plants both when treating dry seeds before sowing and when treating sown seeds in the field. The optimal time period for EMF treatment was determined: treating air-dried seeds with EMFs before sowing for 10–15 min increased germination by 5–18% and the growth rate of seedlings by 2–3 times. The maximum observed effect occurred during the treatment period from 7:00 to 11:00. As a result of changing the balance of phytohormones, the further stimulation of the root system and the assimilation surface of plants was noted due to a 1.5-fold increase in the content of auxins. The density of productive stems, ear length, seed set, and 1000 seed weight increased, which ultimately led to an increase in yield by more than 10% and, in some varieties, to a decrease in the protein content in grains compared to the control variant (by 3–22%), bringing them closer to brewing conditions. Full article

This study examines how employees perceive technology-based demands during the digital transformation process and how these perceptions affect job performance. The research utilized data obtained from 388 experts in the accounting and financial reporting profession, a knowledge-intensive field that heavily employs new technologies (e.g., ERP systems, digital audit tools). The data collected through a convenience sampling method was analyzed using SPSS 27 and SmartPLS 4 software. The findings reveal that the direct effect of technostress on job performance is not significant; however, this stress indirectly contributes to performance through techno-eustress. In this study, techno-eustress refers to the cognitive appraisal of technology-related demands as development-enhancing challenges rather than threats. This concept is theoretically grounded in the broader eustress framework, which views stressors as potentially motivating and growth-promoting when positively interpreted. The model is based on Cognitive Evaluation Theory, the Job Demands–Resources Model, and Self-Determination Theory. This study demonstrates that digital transformation can promote not only operational improvements but also organizational resilience by enhancing employees’ psychological resources and adaptive capacities. By highlighting the mediating role of techno-eustress, this research offers a nuanced perspective on how human-centered cognitive mechanisms can strategically support performance and sustainability in the face of technological disruption—an increasingly relevant area for organizations striving to thrive amid uncertainty. Full article

Hans Selye’s stress concept, first introduced in the 1930s, has undergone substantial evolution, extending beyond biology and medicine to influence diverse academic disciplines. Initially, Selye’s General Adaptation Syndrome (GAS) described nonspecific physiological responses to stressors exclusively in mammals, without addressing other biological systems. Consequently, the concept of stress developed independently in biology and medicine, shaped by distinct physiological contexts. This review provides a historical overview of stress research, highlights both parallels and divergences between the stress responses of plants and animals, and integrates insights from traditional Eastern philosophies. We propose an updated GAS framework that incorporates the dynamic balance among reactive oxygen species (ROS), reactive nitrogen species (RNS), and reactive sulfur species (RSS) within the broader context of oxidative stress. We highlight the ionotropic glutamate receptor (iGluR) family and the transient receptor potential (TRP) channel superfamily as minimal molecular architectures for achieving GAS. This perspective expands the classical stress paradigm, providing new insights into redox biology, interspecies stress adaptation, and evolutionary physiology. Full article

α-ketoglutarate dehydrogenase complex (KGDHc) is a crucial enzyme in the tricarboxylic acid (TCA) cycle that intersects monosaccharides, amino acids, and fatty acid catabolism with oxidative phosphorylation (OxPhos). A key feature of KGDHc is its ability to sense changes in the redox environment through the reversible oxidation of the vicinal lipoic acid thiols of its dihydrolipoamide succinyltransferase (DLST; E2) subunit, which controls its activity and, by extension, OxPhos. This characteristic inculcates KGDHc with redox regulatory properties for the modulation of metabolism and mediating of intra- and intercellular signals. The innate capacity of KGDHc to participate in the regulation of cell redox homeodynamics also occurs through the production of mitochondrial hydrogen peroxide (mtH₂O₂), which is generated by the dihydrolipoamide dehydrogenase (DLD; E3) downstream from the E2 subunit. Reversible covalent redox modification of the E2 subunit controls this mtH₂O₂ production by KGDHc, which not only protects from oxidative distress but also modulates oxidative eustress pathways. The importance of KGDHc in modulating redox homeodynamics is underscored by the pathogenesis of neurological and metabolic disorders that occur due to the hyper-generation of mtH₂O₂ by this enzyme complex. This also implies that the targeted redox modification of the E2 subunit could be a potential therapeutic strategy for limiting the oxidative distress triggered by KGDHc mtH₂O₂ hyper-generation. In this short article, I will discuss recent findings demonstrating KGDHc is a potent mtH₂O₂ source that can trigger the manifestation of several neurological and metabolic diseases, including non-alcoholic fatty liver disease (NAFLD), inflammation, and cancer, and the targeted redox modification of the E2 subunit could alleviate these syndromes. Full article

Chronic oxidative distress results in cellular damage, necessitating adaptive mechanisms for redox balance. The transcription factor nuclear factor erythroid 2-related factor 2 (Nrf2) is pivotal in the regulation of key antioxidant and cytoprotective genes. Under normal conditions, Nrf2 undergoes rapid degradation through polyubiquitination. However, it can be activated during oxidative eustress and distress via modifications of its inhibitor Kelch-like ECH-associated protein 1 (KEAP1). Activation of the Nrf2-Keap1 signaling pathway may decelerate aging-related muscle degeneration, such as sarcopenia and cachexia. In this study, we investigated the efficacy of two muscle-active endogenous factors, creatine and L-β-aminoisobutyric acid (L-BAIBA), as well as two natural phytochemicals, luteolin and silibinin, to induce Nrf2 in the murine myoblast cell line C2C12. Our results revealed that only luteolin significantly enhances Nrf2 activity in both proliferating and differentiated C2C12 cells, leading to increased expression of Nrf2 target genes in proliferating C2C12 cells. In contrast, the other three compounds had either no or only minor effects on Nrf2 activity or target gene expression. Our results underscore the distinct responses of C2C12 cells to different Nrf2 activators, emphasizing the significance of cellular context in their biological effects and highlight luteolin as a potential future treatment option to counteract muscle wasting associated with sarcopenia and cachexia. Full article

Altered levels of reactive oxygen species (ROS) are recognized as one of the key factors in mediating tumor cell survival in the tissue microenvironment, where they play a role in the initiation, progression and recurrence/relapse of colorectal cancer (CRC). Tumor cells can adapt to oxidative stress (OS) using genetic or metabolic reprogramming in the long or short term. In addition, tumor cells defend themselves through positive regulation of antioxidant molecules, enhancing ROS-driven proliferation. Balanced oxidative eustress levels can influence chemotherapy resistance, allowing tumor cells to survive treatment. Secondary effects of chemotherapy include increased ROS production and redox stress, which can kill cancer cells and eliminate drug resistance. Anticancer treatments based on manipulating ROS levels could represent the gold standard in CRC therapy. Therefore, exploring the modulation of the response to OS in deregulated signaling pathways may lead to the development of new personalized CRC treatments to overcome therapy resistance. In this review, we explore the role of ROS in the initiation and progression of CRC and their diagnostic implications as biomarkers of disease. Furthermore, we focused on the involvement of ROS in different CRC therapeutic options, such as surgery, radiotherapy, theranostic imaging, chemotherapy and immunotherapy and other precision medicine approaches. Full article

Stress management during examinations is an issue perpetually concerning students at all levels of education. This research endeavor focuses not merely on the plethora of factors associated with stress during examinations but rather focuses primarily on specific emotions developed during written and oral exams, introducing an original method, based on electroencephalograph (EEG) data. In an attempt to address the role of emotions in stress management, we organized an experiment on a sample of 30 postgraduate students, administering the Trier research protocol. The validated Emotiv Epoc+ device was used, while acquired the data were processed using a combined methodology of network and time series analysis applied in three (3) steps. Initially a descriptive analysis was performed based on the extracted frequencies, while in the second phase a network analysis was conducted to understand the extend of coordination between measured emotions (stress, engagement, interest, relaxation, excitement, and focus), using the tools of centrality. Finally, time series analysis was employed to indicate the level of auto- and cross-correlation between stress and other emotions. Our findings show that (eu)stress plays an imperative role in examination success, as long as it normally fluctuates within middle-range values (on a scale 0–100), and is being restrained by other feelings, such as interest, excitement, and focus Full article

Oxidative stress is universal to all cell types, including cancer. It is elicited by a surplus of reactive oxygen species (ROS) or a reduced cellular ability to defend against those. At low levels (oxidative eustress), this induces altered cellular signaling, while at higher levels (oxidative distress), cellular toxicity and non-specific redox signaling become apparent. While oxidation-induced cell death is a hallmark of many cancer therapies, including ROS-producing radiotherapy, some chemotherapies and targeted therapies, photodynamic therapy, and recently emerging physical modalities such as medical gas plasma (a multi-ROS generating technology), less is known about the transcriptional profiles predisposing cancer cells to oxidative demise. In particular, which genes are associated with resistance or sensitivity to ROS overload and subsequent toxicity has not been systematically investigated. Moreover, it is unclear if there are differences between oxidant types, such as hydrogen peroxide and hypochlorous acid. To this end, we here employed 35 cell lines of various origins (e.g., adenocarcinoma, melanoma, leukemia, squamous cell carcinoma, and neuroblastoma). We first performed in-house transcriptomic analysis to assess baseline transcriptional profiles. Second, all cell lines were exposed to four different ROS concentrations of either hydrogen peroxide, hypochlorous, or gas plasma exposure. Third, correlation analysis was performed to identify genes associated with (i) oxidative stress sensitivity, (ii) oxidative stress resistance, and (iii) similarities and/or differences between the different oxidative stress inducers. Intriguingly, distinct gene sets were found for all treatments, and there was a striking difference between hydrogen peroxide and hypochlorous acid, suggesting different modes of action of both oxidants. Full article

The functionality of redox metabolism is frequently named as an important contributor to the processes of aging and anti-aging. Excessive activation of free radical reactions accompanied by the inability of the antioxidant defense (AOD) mechanisms to control the flow of the reactive oxygen species (ROS) leads to the persistence of oxidative stress, hypoxia, impaired mitochondrial energy function and reduced ATP potential. From a long-term perspective, such changes contribute to the development of chronic diseases and facilitate aging. In turn, preconditioning of a biosystem with small doses of stressful stimuli might cause mobilization of the mechanisms of AOD and control an excessive flow of ROS, which supports optimal functioning of the redox reactions. Those mechanisms are of crucial importance for anti-aging and are also known as a eustress or hormetic response. To ensure continuous support of mild pro-oxidant activity in a metabolic system, close monitoring and timely corrections preventing the development of excessive ROS production are required. The paper introduces the potential of heart rate variability (HRV) as a biomarker of functional and metabolic reserves and a tool to measure stress resilience during aging. The practical approaches to interpretation of HRV are provided based on total power, changes in total power in response to an orthostatic test and activities of all spectral components. It is suggested that the complex of those parameters can reflect the depth of oxidative stress and may be used to guide lifestyle interventions and promote active longevity. Full article